AT4G32295

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010191
Physical Location & Seq
Forward (+)
15592888 .. 15594555
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32295.2

Sequence Viewer

Length: 717 bp
ATGCCGAGGCCAGGGCCAAGACCGTACGACTGTATCAGAAGAGCTTGGCATAGTGACAGACACCAACCCATGAGGGGTTTGCTCATCCAAGAGATTTTCAGGATTGTTTGTGAGATTCACAGCCAATCCACCAGGAAAAACACAGAATGGCAAGAGAAGCTCCCTGTTGTTGTCTTGAGAGCTGAAGAAATCATGTATTCCAAAGCCAATTCTGAGGCTGAGTATATGGACATGAAGACCCTTTTAGACCGTACAAACGACGCCATCAACACAATCATACGACTTGATGAGACCACCGAAACAGGGGAATTTCTTCAGCCTTGTATTGAAGCTGCATTGCATTTGGGGTGCACACCAAGGAGAGCTTCAAGGAGCCAACGGAACATTAACCCGAGATGTTATCTTAGCCAAGACTCAACTAACTTGGACAACATGTTGTCCCAAGTATTCATGAAACCAAACAACTTTGCTCCAAAGAATCTTGCAGTAGCACAAGAGAATTGCCCTGTCTCCAAGTACTCTGCCTACCCTTTATGCTATTCATTCCGGCCAATCAGTGACTCGTGTAAGTCCAAGAACAGTAGACCGGCGAGTCTCATTGACGCAACAAATGGCATTACCTTTGGTGGGTGTGAATGTGATCTATCTCTGCGCTTAGGTCCTCTTGGACCTCCTACTCAAAAACGAAGTAAGATAAGCAGCAACAATAACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

27.0

Weight (kDa)

8.81

Isoelectric Point (pI)

60.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32295 AT4G32295 AT4G32295 AT4G32295
fragaria_vesca FvH4_2g29010 FvH4_2g29010
malus_domestica MD00G1144400.v1.1
prunus_persica Prupe.1G481300_v2.0.a1
pyrus_communis pycom15g11330
rosa_chinensis RchiOBHm_Chr6g0297891
rosa_laevigata RLG00000011553
rosa_multiflora Rmu_sc0011097.1_g000012
rosa_roxburghii Rroxscaffold_7G00170110 Rroxscaffold_7G00178980
rosa_rugosa Rorug06G0276600 Rorug06G0276700
rosa_samantha Rh6AG387200 Rh6BG395600 Rh6CG401400 Rh6DG387900
rosa_wichuraiana Rw6G033730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 591
AccI GTMKAC 1 cut(s) 583
AcoI YGGCCR 1 cut(s) 548
AcsI RAATTY 1 cut(s) 308
AcuI CTGAAG 2 cut(s) 204, 299
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 3 cut(s) 26, 253, 518
AfiI CCNNNNNNNGG 4 cut(s) 11, 74, 303, 627
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 2 cut(s) 329, 369
AjnI CCWGG 2 cut(s) 10, 131
AjuI GAANNNNNNNTTGG 2 cut(s) 349, 381
AluBI AGCT 5 cut(s) 44, 160, 182, 332, 365
AluI AGCT 5 cut(s) 44, 160, 182, 332, 365
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 3 cut(s) 284, 514, 599
Alw44I GTGCAC 1 cut(s) 349
Ama87I CYCGRG 1 cut(s) 391
AoxI GGCC 3 cut(s) 8, 14, 548
ApaLI GTGCAC 1 cut(s) 349
ApeKI GCWGC 2 cut(s) 332, 699
ApoI RAATTY 1 cut(s) 308
Asp700I GAANNNNTTC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 654
AspS9I GGNCC 3 cut(s) 14, 659, 668
AvaI CYCGRG 1 cut(s) 391
AvaII GGWCC 2 cut(s) 659, 668
BaeGI GKGCMC 1 cut(s) 353
BaeI ACNNNNGTAYC 2 cut(s) 16, 49
BauI CACGAG 1 cut(s) 562
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 2 cut(s) 319, 711
BccI CCATC 1 cut(s) 272
BciT130I CCWGG 2 cut(s) 12, 133
BcoDI GTCTC 3 cut(s) 284, 514, 599
BisI GCNGC 2 cut(s) 333, 700
BlsI GCNGC 2 cut(s) 334, 701
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 2 cut(s) 12, 133
Bme18I GGWCC 2 cut(s) 659, 668
BmeT110I CYCGRG 1 cut(s) 391
BmgT120I GGNCC 3 cut(s) 14, 659, 668
BmiI GGNNCC 1 cut(s) 374
BmrFI CCNGG 2 cut(s) 12, 133
BpiI GAAGAC 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 655
BpuEI CTTGAG 1 cut(s) 196
BsaHI GRCGYC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 3 cut(s) 5, 11, 356
Bsc4I CCNNNNNNNGG 4 cut(s) 11, 74, 303, 627
Bse118I RCCGGY 1 cut(s) 586
Bse3DI GCAATG 1 cut(s) 335
BseBI CCWGG 2 cut(s) 12, 133
BseDI CCNNGG 3 cut(s) 5, 11, 356
BseGI GGATG 1 cut(s) 84
BseLI CCNNNNNNNGG 4 cut(s) 11, 74, 303, 627
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 2 cut(s) 204, 210
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 2 cut(s) 319, 711
BshFI GGCC 3 cut(s) 10, 16, 550
BsiHKAI GWGCWC 1 cut(s) 353
BsiHKCI CYCGRG 1 cut(s) 391
BsiSI CCGG 2 cut(s) 547, 587
BsiWI CGTACG 1 cut(s) 24
BslFI GGGAC 1 cut(s) 424
BslI CCNNNNNNNGG 4 cut(s) 11, 74, 303, 627
BsmAI GTCTC 3 cut(s) 284, 514, 599
BsmFI GGGAC 1 cut(s) 424
BsnI GGCC 3 cut(s) 10, 16, 550
Bso31I GGTCTC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 1 cut(s) 640
BspANI GGCC 3 cut(s) 10, 16, 550
BspCNI CTCAG 2 cut(s) 205, 211
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 1 cut(s) 374
BspQI GCTCTTC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 335
BsrFI RCCGGY 1 cut(s) 586
BssAI RCCGGY 1 cut(s) 586
BssECI CCNNGG 3 cut(s) 5, 11, 356
BssMI GATC 1 cut(s) 640
BssNI GRCGYC 1 cut(s) 261
BssSI CACGAG 1 cut(s) 562
BssT1I CCWWGG 1 cut(s) 356
Bst2BI CACGAG 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 12, 133
Bst4CI ACNGT 4 cut(s) 24, 32, 251, 581
Bst6I CTCTTC 1 cut(s) 34
BstACI GRCGYC 1 cut(s) 261
BstDEI CTNAG 4 cut(s) 213, 219, 404, 655
BstF5I GGATG 1 cut(s) 84
BstHHI GCGC 1 cut(s) 654
BstKTI GATC 1 cut(s) 643
BstMAI GTCTC 3 cut(s) 284, 514, 599
BstMBI GATC 1 cut(s) 640
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstNI CCWGG 2 cut(s) 12, 133
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 2 cut(s) 10, 131
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 319, 711
BstV2I GAAGAC 1 cut(s) 242
BsuRI GGCC 3 cut(s) 10, 16, 550
BtsCI GGATG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 562
CciI TCATGA 1 cut(s) 450
CfoI GCGC 1 cut(s) 654
Cfr10I RCCGGY 1 cut(s) 586
Cfr13I GGNCC 3 cut(s) 14, 659, 668
CseI GACGC 2 cut(s) 269, 611
Csp6I GTAC 3 cut(s) 25, 252, 517
CviAII CATG 5 cut(s) 70, 193, 232, 433, 451
CviQI GTAC 3 cut(s) 25, 252, 517
DdeI CTNAG 4 cut(s) 213, 219, 404, 655
DpnI GATC 1 cut(s) 642
DpnII GATC 1 cut(s) 640
DrdI GACNNNNNNGTC 1 cut(s) 591
DseDI GACNNNNNNGTC 1 cut(s) 591
EaeI YGGCCR 1 cut(s) 548
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 2 cut(s) 659, 668
Eco57I CTGAAG 2 cut(s) 204, 299
Eco88I CYCGRG 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 659
EcoRII CCWGG 2 cut(s) 10, 131
EcoT14I CCWWGG 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 5 cut(s) 73, 196, 235, 436, 454
FalI AAGNNNNNCTT 2 cut(s) 349, 381
FaqI GGGAC 1 cut(s) 424
FatI CATG 5 cut(s) 69, 192, 231, 432, 450
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 2 cut(s) 333, 700
FokI GGATG 1 cut(s) 71
Fsp4HI GCNGC 2 cut(s) 333, 700
GlaI GCGC 1 cut(s) 653
GluI GCNGC 2 cut(s) 333, 700
HaeIII GGCC 3 cut(s) 10, 16, 550
HapII CCGG 2 cut(s) 547, 587
HgaI GACGC 2 cut(s) 269, 611
HhaI GCGC 1 cut(s) 654
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 5 cut(s) 73, 196, 235, 436, 454
Hin6I GCGC 1 cut(s) 652
HinP1I GCGC 1 cut(s) 652
HinfI GANTC 5 cut(s) 115, 413, 478, 560, 592
HpaII CCGG 2 cut(s) 547, 587
Hpy166II GTNNAC 2 cut(s) 351, 584
Hpy188I TCNGA 2 cut(s) 38, 214
Hpy188III TCNNGA 3 cut(s) 100, 175, 451
Hpy8I GTNNAC 2 cut(s) 351, 584
Hpy99I CGWCG 1 cut(s) 263
HpyCH4III ACNGT 4 cut(s) 24, 32, 251, 581
HpyCH4V TGCA 4 cut(s) 335, 340, 351, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 4 cut(s) 213, 219, 404, 655
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 5 cut(s) 73, 196, 235, 436, 454
HspAI GCGC 1 cut(s) 652
Kzo9I GATC 1 cut(s) 640
LguI GCTCTTC 1 cut(s) 34
LmnI GCTCC 3 cut(s) 165, 372, 475
LpnPI CCDG 9 cut(s) 24, 85, 118, 145, 177, 288, 519, 560, 600
Lsp1109I GCAGC 2 cut(s) 319, 711
MaeIII GTNAC 2 cut(s) 53, 557
MalI GATC 1 cut(s) 642
MboI GATC 1 cut(s) 640
MboII GAAGA 4 cut(s) 51, 197, 247, 305
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 3 cut(s) 208, 308, 499
MlyI GAGTC 3 cut(s) 407, 554, 601
MnlI CCTC 4 cut(s) 66, 208, 672, 681
MroXI GAANNNNTTC 1 cut(s) 312
MseI TTAA 1 cut(s) 387
MspI CCGG 2 cut(s) 547, 587
MspR9I CCNGG 2 cut(s) 12, 133
MvaI CCWGG 2 cut(s) 12, 133
MwoI GCNNNNNNNGC 1 cut(s) 157
NdeII GATC 1 cut(s) 640
NlaIII CATG 5 cut(s) 73, 196, 235, 436, 454
NlaIV GGNNCC 1 cut(s) 374
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 53, 557
NspI RCATGY 1 cut(s) 436
PagI TCATGA 1 cut(s) 450
PciI ACATGT 1 cut(s) 432
PciSI GCTCTTC 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 2 cut(s) 115, 478
Pfl23II CGTACG 1 cut(s) 24
PkrI GCNGC 2 cut(s) 334, 701
PleI GAGTC 3 cut(s) 407, 554, 600
PpsI GAGTC 3 cut(s) 407, 554, 600
PpuMI RGGWCCY 1 cut(s) 659
PscI ACATGT 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 659
Psp6I CCWGG 2 cut(s) 10, 131
PspGI CCWGG 2 cut(s) 10, 131
PspLI CGTACG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 374
PspPI GGNCC 3 cut(s) 14, 659, 668
PspPPI RGGWCCY 1 cut(s) 659
RsaI GTAC 3 cut(s) 26, 253, 518
RsaNI GTAC 3 cut(s) 25, 252, 517
SapI GCTCTTC 1 cut(s) 34
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 2 cut(s) 333, 700
Sau3AI GATC 1 cut(s) 640
Sau96I GGNCC 3 cut(s) 14, 659, 668
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 3 cut(s) 407, 554, 601
ScrFI CCNGG 2 cut(s) 12, 133
SduI GDGCHC 1 cut(s) 353
SetI ASST 8 cut(s) 46, 162, 184, 334, 367, 623, 661, 673
SinI GGWCC 2 cut(s) 659, 668
SmlI CTYRAG 1 cut(s) 175
SmoI CTYRAG 1 cut(s) 175
Sse9I AATT 3 cut(s) 208, 308, 499
StyD4I CCNGG 2 cut(s) 10, 131
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 4 cut(s) 24, 32, 251, 581
TasI AATT 3 cut(s) 208, 308, 499
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 2 cut(s) 115, 478
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 1 cut(s) 562
TseFI GTSAC 2 cut(s) 53, 557
TseI GCWGC 2 cut(s) 332, 699
Tsp45I GTSAC 2 cut(s) 53, 557
TspDTI ATGAA 4 cut(s) 248, 439, 467, 531
TspGWI ACGGA 1 cut(s) 394
TspRI CASTG 1 cut(s) 562
VneI GTGCAC 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 659, 668
XapI RAATTY 1 cut(s) 308
XceI RCATGY 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 583
XmnI GAANNNNTTC 1 cut(s) 312
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.