Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009795

Physical Location & Seq

Genomic Coordinates Forward (+)

15681017 .. 15685339

Gene Structure 4323 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G32500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2643 bp

ATGGGTATTGAGAAGAGGAAGAAGATGGTGTGGTTTTGGCCGGAGAAGCACGAGGGTGGAGTGATTAAGGAGGCGGAGGATGTGGCGGCGGAACATATATCGAGAGAAGGTACAATGAGTCATTACAGTTTTAGCAAAGGTCTTCTTCCGCCGCTTGGAGTCGGAGCCACCGCTCGCTCTAGCCGTCACATTAAGCTCCGCTGCTTCATCGTCTCTCCTTTCGATCCTCGCTACAGAGCTTGGGATTGGTTTCTAGTGATTTTGGTGCTATACACAGCGTGGGCATCTCCTTTTGAGTTCGGGTTCCTACAAACACCAAGAGCACCTCTCAGTATTCTTGATAACGTGGTGAATGGATTTTTCGCTGTCGACATTGTTCTGACATTCTTTGTTGCCTTCTTGGACAAGGCAACTTATCTCCTAGTCGATGATCCCAAGAGAATAGCTTGGAGATATACTTCGACTTGGCTGATTTTTGATGTGGTATCTACAGTTCCTTATGAACTTTTTGGCAGCTTATTGCATAACACTATTCAAGGATATGGAATTTTCAGTATGTTGCGTTTATGGCGTCTGCACAGGGTCAGTAAATGTTTTGCCAGATTGGAGAAAGATCGAAAATACAACTACTTCTGGATTCGATGCACGAAGCTTCTCCTCGTTTCTCTCTTTGTGGTTCATTGTGGCGCCTGCTTCTGCTATTCTATTGCTGCACATTATCCAGACCCTTCAATGACATTTATGGCACTTGCGGAGGCAAACTGGAAACAGAAATCTTTGCTTATCCGGTATGTTACAGCAATGTATTGGTCCATAACAACCTTCTCCACAACGGGTTATGGTGATATACATGGTAATAATGCAGAAGAGAGGGCCTTCATTCTCTTCTACATGATCTTTAATCTTGGATTGCTAGCTTATATAATTGGTAACATGACAAACTTGGTGGTTCACGTGACCAGTCGCACCAGAAACTTTAGAGATACCATTCAAGCTGCCTCAGCGTTTGCTCAGAGGAACAATCTTCCACTGGGCTTGCAAGAACAGATGGTAGCTCATTTGTCTTTGAGGTACAGAACCGATTCAGAAGGCCTGCAGCAGCAAGAAATTATTGATTCCCTCCCCAAGGCTATTCGTTCCAGCATATCGCATTATCTGTTCTACGAAGTTGTTGACAAAACCTACTTATTCCATGGAATATCCAATGATCTTCTTTTCCAGTTGGTCTCTGAGATGAAGGCAGAGTATTTTCCTCCTAAAGAAGATGTGATTTTGCGGAATGAAGCACCGTCAGACTTTTACATAATGGTCACAGGAGCTGTTGACATCATTGCGCGTGTGAATGGAGTGGATCAGGTAGTTGGTGAAGCACAGACCGGCCATGTTTTTGGTGAAGTTGGGGTTCTATGTTACAGGCCACAGTTGTTCACAGTTCGAACCAAACGATTGAGTCAATTGCTTCGTCTGAACCGTACAGCATTCTTAAATCTTGTTCAGGCAAACGTTGGTGATGGGGCAATAATCATGAATAATCTTCTTCAGCATCTGAAAGACTCAACAGATCCAGTGATGAAAGGCATTTTGGCTGAGACAGAACTCATGTTGGCTCAGGGGAAAATGGATTTACCTCTCAGCTTATGTTTTGCAGCAGCCAGAGGAGACGATTTACTGCTGCATCAGTTGCTGAAACGAGGCTCAAACCCTAACGAAACCGATAAGAACGGTCGAACCGCGTTGCATATAGCAGCATCAAAAGGAAGCCAATATTGTGTTGTATTACTTCTCGAGCATGGAGCAGATCCTAACATTAGAGATTCAGAAGGTAGTGTACCATTATGGGAAGCAATCATTGGGAGACATGAAGAAAATGCTAAACTCTTATCAGAAAATGGCGCAACGCTAAGCTTCGACACAGTAGGCTATTTCTCGTGCTTAGCCGTTGGACAAAACAACTTAAATGCGCTCAAAGACATTGTAAAATACGGAGGAGATATCTCACTCTCAGATGTCAACGGAACCACAGCTCTGCACAGAGCAGTCTCAGAAGGAAACTTGGAGATTGTACAATTCTTGTTAGAGAAAGGAGCTGATATGGACAAGCCAGATGTTTATGGTTGGACGGCTCGTGCTCTTGCTGAGCATCAAGGACATGAGGATATCAAGGCTTTGTTTTATAATCAGAGACCAGTGGAGAGGAAAACGATACTTGTTTCTGGCACACCAGAGATTAAACCATTGATGAAACATTCCAGTGAGCCTGTAATGACTCACCATCATTCAAGGGAAGCAATGCCGCCTCTGGCCAGAGCCGTTTCTCAGAGACGGAAGCTCAGTAATTTCAAGAACTCGCTGTTTGGTATCATGTCAGCTGCTAAAACTGGTGATGAAGGAGGAGCATCTACCAGGACTGGAATAAGTGAAGGAGTTGGTGGGGTTTATCCGGCGAGAGTGACAATCAGTGGTGAGGCTTCCTCCTCCGGTAAAGTAGTAAAGTTACCGGATTCTCTTGAAGAATTAATTGAAATTGGTGAGAAGAAGCTGGGATTCGTAGCCACAAAGATTCTGAGCAGAGAAGGAGCTGAAATCGACGACATCAGGATTATAAGAGATGGCGATTTTCTCCTTCTTCTTAAGGTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008324 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010035 GO:0010053 GO:0010054 GO:0010107 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016049 GO:0021700 GO:0022622 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0034220 GO:0040007 GO:0042221 GO:0042391 GO:0042802 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071804 GO:0071805 GO:0071944 GO:0080147 GO:0090333 GO:0090558 GO:0090627 GO:0098655 GO:0098660 GO:0098662 GO:0099094 GO:0099402 GO:1901700 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

880

Amino Acids

98.5

Weight (kDa)

7.37

Isoelectric Point (pI)

33.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	78 - 323	7.8e-43	Ion transport protein
Ion_trans_2	PF07885	260 - 316	1.1e-11	Ion channel
cNMP_binding	PF00027	416 - 501	8.1e-13	Cyclic nucleotide-binding domain
Ank_2	PF12796	546 - 633	4.1e-16	Ankyrin repeats (3 copies)
Ank_4	PF13637	546 - 586	1.8e-07	Ankyrin repeats (many copies)
Ank_5	PF13857	561 - 612	5.2e-09	Ankyrin repeats (many copies)
Ank	PF00023	574 - 605	1.8e-09	Ankyrin repeat
Ank_2	PF12796	645 - 724	1.7e-12	Ankyrin repeats (3 copies)
Ank_4	PF13637	646 - 692	1.3e-07	Ankyrin repeats (many copies)
Ank_5	PF13857	660 - 712	1.1e-06	Ankyrin repeats (many copies)
Ank	PF00023	671 - 702	6.3e-08	Ankyrin repeat
KHA	PF11834	816 - 876	1.1e-20	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009795)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25600 AT4G32500
fragaria_vesca	FvH4_2g29700 FvH4_2g29700 FvH4_2g29700
malus_domestica	MD08G1142800.v1.1 MD15G1119300.v1.1
prunus_persica	Prupe.1G472600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0298701
rosa_laevigata	RLG00000011492
rosa_multiflora	Rmu_co8298943.1_g000001 Rmu_sc0008099.1_g000002 Rmu_sc0008142.1_g000002 Rmu_sc0032030.1_g000001
rosa_roxburghii	Rroxscaffold_7G00169400
rosa_rugosa	Rorug06G0282700
rosa_samantha	Rh6AG393800 Rh6BG401600 Rh6CG407500 Rh6DG394100
rosa_wichuraiana	Rw6G034430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2175, 2603
AccB1I	GGYRCC	1 cut(s)	686
AccBSI	CCGCTC	1 cut(s)	173
AccI	GTMKAC	1 cut(s)	369
AccII	CGCG	2 cut(s)	1336, 1733
AclI	AACGTT	1 cut(s)	1503
AclWI	GGATC	5 cut(s)	218, 425, 1359, 1556, 1793
AcoI	YGGCCR	3 cut(s)	38, 1378, 2301
AcsI	RAATTY	1 cut(s)	546
AcuI	CTGAAG	1 cut(s)	1523
AcvI	CACGTG	1 cut(s)	955
AcyI	GRCGYC	2 cut(s)	571, 687
AdeI	CACNNNGTG	1 cut(s)	279
AfaI	GTAC	5 cut(s)	112, 1073, 1474, 1830, 2064
AfiI	CCNNNNNNNGG	2 cut(s)	155, 1126
AflII	CTTAAG	1 cut(s)	2630
AgsI	TTSAA	7 cut(s)	536, 732, 992, 2280, 2341, 2510, 2522
AjnI	CCWGG	1 cut(s)	2402
AjuI	GAANNNNNNNTTGG	4 cut(s)	1565, 1597, 1833, 1865
AleI	CACNNNNGTG	1 cut(s)	54
Alw21I	GWGCWC	2 cut(s)	325, 2131
Alw26I	GTCTC	8 cut(s)	217, 1231, 1583, 1653, 1849, 2044, 2176, 2314
AlwI	GGATC	5 cut(s)	218, 425, 1359, 1556, 1793
AlwNI	CAGNNNCTG	3 cut(s)	1319, 1546, 1684
Ama87I	CYCGRG	1 cut(s)	1784
AoxI	GGCC	6 cut(s)	38, 873, 1090, 1378, 1415, 2301
ApoI	RAATTY	1 cut(s)	546
AseI	ATTAAT	1 cut(s)	2516
Asp700I	GAANNNNTTC	1 cut(s)	1081
AspLEI	GCGC	4 cut(s)	689, 1336, 1895, 1963
AspS9I	GGNCC	2 cut(s)	810, 873
AsuHPI	GGTGA	9 cut(s)	361, 854, 1376, 1403, 1520, 2261, 2393, 2474, 2540
AsuII	TTCGAA	1 cut(s)	1435
AsuNHI	GCTAGC	1 cut(s)	913
AvaI	CYCGRG	1 cut(s)	1784
AvaII	GGWCC	1 cut(s)	810
BalI	TGGCCA	1 cut(s)	2303
BanI	GGYRCC	1 cut(s)	686
BarI	GAAGNNNNNNTAC	2 cut(s)	1812, 1844
BauI	CACGAG	3 cut(s)	50, 1927, 2124
BbrPI	CACGTG	1 cut(s)	955
BbsI	GAAGAC	1 cut(s)	134
Bbv12I	GWGCWC	2 cut(s)	325, 2131
BbvCI	CCTCAGC	1 cut(s)	1000
BccI	CCATC	5 cut(s)	19, 1042, 1505, 2280, 2603
BceAI	ACGGC	4 cut(s)	168, 1922, 2136, 2294
BciT130I	CCWGG	1 cut(s)	2404
BcoDI	GTCTC	8 cut(s)	217, 1231, 1583, 1653, 1849, 2044, 2176, 2314
BfaI	CTAG	4 cut(s)	180, 254, 422, 914
BfmI	CTRYAG	3 cut(s)	232, 489, 1094
BfoI	RGCGCY	1 cut(s)	690
BfrI	CTTAAG	1 cut(s)	2630
BlpI	GCTNAGC	3 cut(s)	1901, 1933, 2136
Bme1390I	CCNGG	1 cut(s)	2404
Bme18I	GGWCC	1 cut(s)	810
BmeT110I	CYCGRG	1 cut(s)	1784
BmgT120I	GGNCC	2 cut(s)	810, 873
BmiI	GGNNCC	4 cut(s)	166, 305, 688, 2017
BmrFI	CCNGG	1 cut(s)	2404
BmrI	ACTGGG	1 cut(s)	1040
BmsI	GCATC	7 cut(s)	293, 632, 1552, 1684, 1757, 2149, 2405
BmtI	GCTAGC	1 cut(s)	917
BmuI	ACTGGG	1 cut(s)	1040
BpiI	GAAGAC	1 cut(s)	134
BplI	GAGNNNNNCTC	4 cut(s)	312, 344, 2456, 2488
Bpu10I	CCTNAGC	2 cut(s)	1000, 1608
Bpu1102I	GCTNAGC	3 cut(s)	1901, 1933, 2136
Bpu14I	TTCGAA	1 cut(s)	1435
BsaAI	YACGTR	1 cut(s)	955
BsaHI	GRCGYC	2 cut(s)	571, 687
BsaI	GGTCTC	2 cut(s)	1231, 2176
BsaJI	CCNNGG	2 cut(s)	1125, 1192
BsaWI	WCCGGW	3 cut(s)	786, 2477, 2497
Bsc4I	CCNNNNNNNGG	2 cut(s)	155, 1126
Bse118I	RCCGGY	1 cut(s)	1376
Bse1I	ACTGG	9 cut(s)	767, 960, 1035, 1219, 1565, 2186, 2250, 2383, 2413
Bse3DI	GCAATG	3 cut(s)	807, 1329, 2295
BseBI	CCWGG	1 cut(s)	2404
BseDI	CCNNGG	2 cut(s)	1125, 1192
BseGI	GGATG	1 cut(s)	85
BseLI	CCNNNNNNNGG	2 cut(s)	155, 1126
BseMI	GCAATG	3 cut(s)	807, 1329, 2295
BseNI	ACTGG	9 cut(s)	767, 960, 1035, 1219, 1565, 2186, 2250, 2383, 2413
BseRI	GAGGAG	5 cut(s)	647, 1671, 2001, 2406, 2464
BseYI	CCCAGC	1 cut(s)	2539
BsgI	GTGCAG	3 cut(s)	560, 696, 2012
Bsh1236I	CGCG	2 cut(s)	1336, 1733
Bsh1285I	CGRYCG	1 cut(s)	1726
BshFI	GGCC	6 cut(s)	40, 875, 1092, 1380, 1417, 2303
BshNI	GGYRCC	1 cut(s)	686
BsiEI	CGRYCG	1 cut(s)	1726
BsiHKAI	GWGCWC	2 cut(s)	325, 2131
BsiHKCI	CYCGRG	1 cut(s)	1784
BsiSI	CCGG	6 cut(s)	41, 787, 1377, 2441, 2478, 2498
BslI	CCNNNNNNNGG	2 cut(s)	155, 1126
BsmAI	GTCTC	8 cut(s)	217, 1231, 1583, 1653, 1849, 2044, 2176, 2314
BsmBI	CGTCTC	3 cut(s)	217, 1653, 2314
BsmI	GAATGC	1 cut(s)	1478
BsnI	GGCC	6 cut(s)	40, 875, 1092, 1380, 1417, 2303
Bso31I	GGTCTC	2 cut(s)	1231, 2176
BsoBI	CYCGRG	1 cut(s)	1784
Bsp119I	TTCGAA	1 cut(s)	1435
Bsp1286I	GDGCHC	2 cut(s)	325, 2131
Bsp1407I	TGTACA	1 cut(s)	2062
Bsp143I	GATC	8 cut(s)	223, 430, 613, 894, 1207, 1351, 1561, 1798
Bsp1720I	GCTNAGC	3 cut(s)	1901, 1933, 2136
Bsp19I	CCATGG	1 cut(s)	1192
BspANI	GGCC	6 cut(s)	40, 875, 1092, 1380, 1417, 2303
BspFNI	CGCG	2 cut(s)	1336, 1733
BspHI	TCATGA	1 cut(s)	1524
BspLI	GGNNCC	4 cut(s)	166, 305, 688, 2017
BspMAI	CTGCAG	1 cut(s)	1098
BspOI	GCTAGC	1 cut(s)	917
BspPI	GGATC	5 cut(s)	218, 425, 1359, 1556, 1793
BspT104I	TTCGAA	1 cut(s)	1435
BspT107I	GGYRCC	1 cut(s)	686
BspTI	CTTAAG	1 cut(s)	2630
BspTNI	GGTCTC	2 cut(s)	1231, 2176
BsrBI	CCGCTC	1 cut(s)	173
BsrDI	GCAATG	3 cut(s)	807, 1329, 2295
BsrFI	RCCGGY	1 cut(s)	1376
BsrGI	TGTACA	1 cut(s)	2062
BsrI	ACTGG	9 cut(s)	767, 960, 1035, 1219, 1565, 2186, 2250, 2383, 2413
BssAI	RCCGGY	1 cut(s)	1376
BssECI	CCNNGG	2 cut(s)	1125, 1192
BssMI	GATC	8 cut(s)	223, 430, 613, 894, 1207, 1351, 1561, 1798
BssNI	GRCGYC	2 cut(s)	571, 687
BssSI	CACGAG	3 cut(s)	50, 1927, 2124
BssT1I	CCWWGG	2 cut(s)	1125, 1192
Bst2BI	CACGAG	3 cut(s)	50, 1927, 2124
Bst2UI	CCWGG	1 cut(s)	2404
Bst4CI	ACNGT	8 cut(s)	128, 493, 1290, 1422, 1432, 1472, 1724, 1915
Bst6I	CTCTTC	3 cut(s)	8, 861, 890
BstACI	GRCGYC	2 cut(s)	571, 687
BstAFI	CTTAAG	1 cut(s)	2630
BstAPI	GCANNNNNTGC	1 cut(s)	1681
BstAUI	TGTACA	1 cut(s)	2062
BstBAI	YACGTR	1 cut(s)	955
BstBI	TTCGAA	1 cut(s)	1435
BstC8I	GCNNGC	5 cut(s)	175, 691, 915, 1037, 1094
BstDSI	CCRYGG	1 cut(s)	1192
BstENI	CCTNNNNNAGG	1 cut(s)	1124
BstF5I	GGATG	1 cut(s)	85
BstFNI	CGCG	2 cut(s)	1336, 1733
BstH2I	RGCGCY	1 cut(s)	690
BstHHI	GCGC	4 cut(s)	689, 1336, 1895, 1963
BstKTI	GATC	8 cut(s)	226, 433, 616, 897, 1210, 1354, 1564, 1801
BstMAI	GTCTC	8 cut(s)	217, 1231, 1583, 1653, 1849, 2044, 2176, 2314
BstMBI	GATC	8 cut(s)	223, 430, 613, 894, 1207, 1351, 1561, 1798
BstMCI	CGRYCG	1 cut(s)	1726
BstMWI	GCNNNNNNNGC	4 cut(s)	46, 568, 1001, 1681
BstNI	CCWGG	1 cut(s)	2404
BstSCI	CCNGG	1 cut(s)	2402
BstSFI	CTRYAG	3 cut(s)	232, 489, 1094
BstUI	CGCG	2 cut(s)	1336, 1733
BstV2I	GAAGAC	1 cut(s)	134
BstX2I	RGATCY	2 cut(s)	1561, 1798
BstXI	CCANNNNNNTGG	1 cut(s)	1388
BstYI	RGATCY	2 cut(s)	1561, 1798
BsuRI	GGCC	6 cut(s)	40, 875, 1092, 1380, 1417, 2303
BtgI	CCRYGG	1 cut(s)	1192
BtsCI	GGATG	1 cut(s)	85
BtsIMutI	CAGTG	5 cut(s)	1028, 1572, 2193, 2257, 2464
Cac8I	GCNNGC	5 cut(s)	175, 691, 915, 1037, 1094
CaiI	CAGNNNCTG	3 cut(s)	1319, 1546, 1684
CciI	TCATGA	1 cut(s)	1524
CfoI	GCGC	4 cut(s)	689, 1336, 1895, 1963
Cfr10I	RCCGGY	1 cut(s)	1376
Cfr13I	GGNCC	2 cut(s)	810, 873
CseI	GACGC	1 cut(s)	560
Csp6I	GTAC	5 cut(s)	111, 1072, 1473, 1829, 2063
CspCI	CAANNNNNGTGG	4 cut(s)	927, 962, 1017, 1052
CviQI	GTAC	5 cut(s)	111, 1072, 1473, 1829, 2063
DinI	GGCGCC	1 cut(s)	688
DpnI	GATC	8 cut(s)	225, 432, 615, 896, 1209, 1353, 1563, 1800
DpnII	GATC	8 cut(s)	223, 430, 613, 894, 1207, 1351, 1561, 1798
DraIII	CACNNNGTG	1 cut(s)	279
EaeI	YGGCCR	3 cut(s)	38, 1378, 2301
Eam1104I	CTCTTC	3 cut(s)	8, 861, 890
EarI	CTCTTC	3 cut(s)	8, 861, 890
EciI	GGCGGA	3 cut(s)	89, 104, 138
Eco130I	CCWWGG	2 cut(s)	1125, 1192
Eco147I	AGGCCT	1 cut(s)	1092
Eco31I	GGTCTC	2 cut(s)	1231, 2176
Eco32I	GATATC	2 cut(s)	1993, 2158
Eco47I	GGWCC	1 cut(s)	810
Eco57I	CTGAAG	1 cut(s)	1523
Eco72I	CACGTG	1 cut(s)	955
Eco88I	CYCGRG	1 cut(s)	1784
EcoNI	CCTNNNNNAGG	1 cut(s)	1124
EcoO109I	RGGNCCY	1 cut(s)	873
EcoRII	CCWGG	1 cut(s)	2402
EcoRV	GATATC	2 cut(s)	1993, 2158
EcoT14I	CCWWGG	2 cut(s)	1125, 1192
EgeI	GGCGCC	1 cut(s)	688
EheI	GGCGCC	1 cut(s)	688
ErhI	CCWWGG	2 cut(s)	1125, 1192
Esp3I	CGTCTC	3 cut(s)	217, 1653, 2314
FalI	AAGNNNNNCTT	2 cut(s)	129, 161
FblI	GTMKAC	1 cut(s)	369
FokI	GGATG	1 cut(s)	92
FspBI	CTAG	4 cut(s)	180, 254, 422, 914
GlaI	GCGC	4 cut(s)	688, 1335, 1894, 1962
GsaI	CCCAGC	1 cut(s)	2543
HaeII	RGCGCY	1 cut(s)	690
HaeIII	GGCC	6 cut(s)	40, 875, 1092, 1380, 1417, 2303
HapII	CCGG	6 cut(s)	41, 787, 1377, 2441, 2478, 2498
HgaI	GACGC	1 cut(s)	560
HhaI	GCGC	4 cut(s)	689, 1336, 1895, 1963
Hin1I	GRCGYC	2 cut(s)	571, 687
Hin6I	GCGC	4 cut(s)	687, 1334, 1893, 1961
HinP1I	GCGC	4 cut(s)	687, 1334, 1893, 1961
HincII	GTYRAC	4 cut(s)	370, 1174, 1324, 2011
HindII	GTYRAC	4 cut(s)	370, 1174, 1324, 2011
HindIII	AAGCTT	2 cut(s)	650, 1903
HpaII	CCGG	6 cut(s)	41, 787, 1377, 2441, 2478, 2498
HphI	GGTGA	9 cut(s)	361, 854, 1376, 1403, 1520, 2261, 2393, 2474, 2540
Hpy166II	GTNNAC	7 cut(s)	370, 952, 1174, 1324, 1428, 1829, 2011
Hpy8I	GTNNAC	7 cut(s)	370, 952, 1174, 1324, 1428, 1829, 2011
Hpy99I	CGWCG	1 cut(s)	2591
HpyCH4III	ACNGT	8 cut(s)	128, 493, 1290, 1422, 1432, 1472, 1724, 1915
HpyCH4IV	ACGT	3 cut(s)	345, 954, 1503
HpyF10VI	GCNNNNNNNGC	4 cut(s)	46, 568, 1001, 1681
HpySE526I	ACGT	3 cut(s)	345, 954, 1503
Hsp92I	GRCGYC	2 cut(s)	571, 687
HspAI	GCGC	4 cut(s)	687, 1334, 1893, 1961
KasI	GGCGCC	1 cut(s)	686
Kzo9I	GATC	8 cut(s)	223, 430, 613, 894, 1207, 1351, 1561, 1798
LmnI	GCTCC	7 cut(s)	164, 201, 1316, 1793, 2084, 2393, 2576
LweI	GCATC	7 cut(s)	293, 632, 1552, 1684, 1757, 2149, 2405
MaeI	CTAG	4 cut(s)	180, 254, 422, 914
MaeII	ACGT	3 cut(s)	345, 954, 1503
MaeIII	GTNAC	8 cut(s)	185, 793, 929, 955, 1309, 1409, 2449, 2493
MalI	GATC	8 cut(s)	225, 432, 615, 896, 1209, 1353, 1563, 1800
MbiI	CCGCTC	1 cut(s)	173
MboI	GATC	8 cut(s)	223, 430, 613, 894, 1207, 1351, 1561, 1798
MfeI	CAATTG	1 cut(s)	1454
MflI	RGATCY	2 cut(s)	1561, 1798
MhlI	GDGCHC	2 cut(s)	325, 2131
MlsI	TGGCCA	1 cut(s)	2303
MluCI	AATT	9 cut(s)	546, 924, 1107, 1454, 2066, 2335, 2513, 2517, 2523
MluNI	TGGCCA	1 cut(s)	2303
Mly113I	GGCGCC	1 cut(s)	687
MlyI	GAGTC	5 cut(s)	127, 168, 1459, 1547, 2260
MmeI	TCCRAC	3 cut(s)	142, 1921, 2096
Mox20I	TGGCCA	1 cut(s)	2303
MroXI	GAANNNNTTC	1 cut(s)	1081
MscI	TGGCCA	1 cut(s)	2303
MseI	TTAA	8 cut(s)	66, 192, 900, 1484, 1955, 2229, 2516, 2631
MslI	CAYNNNNRTG	2 cut(s)	54, 2250
Msp20I	TGGCCA	1 cut(s)	2303
MspA1I	CMGCKG	2 cut(s)	201, 2369
MspCI	CTTAAG	1 cut(s)	2630
MspI	CCGG	6 cut(s)	41, 787, 1377, 2441, 2478, 2498
MspR9I	CCNGG	1 cut(s)	2404
MunI	CAATTG	1 cut(s)	1454
Mva1269I	GAATGC	1 cut(s)	1478
MvaI	CCWGG	1 cut(s)	2404
MvnI	CGCG	2 cut(s)	1336, 1733
MwoI	GCNNNNNNNGC	4 cut(s)	46, 568, 1001, 1681
NarI	GGCGCC	1 cut(s)	687
NcoI	CCATGG	1 cut(s)	1192
NdeII	GATC	8 cut(s)	223, 430, 613, 894, 1207, 1351, 1561, 1798
NheI	GCTAGC	1 cut(s)	913
NlaIV	GGNNCC	4 cut(s)	166, 305, 688, 2017
NmuCI	GTSAC	4 cut(s)	185, 955, 1309, 2449
NspV	TTCGAA	1 cut(s)	1435
OliI	CACNNNNGTG	1 cut(s)	54
PaeR7I	CTCGAG	1 cut(s)	1784
PagI	TCATGA	1 cut(s)	1524
PceI	AGGCCT	1 cut(s)	1092
PcsI	WCGNNNNNNNCGW	3 cut(s)	181, 1441, 1468
PctI	GAATGC	1 cut(s)	1478
PdmI	GAANNNNTTC	1 cut(s)	1081
PfeI	GAWTC	7 cut(s)	637, 1082, 1115, 1814, 2501, 2544, 2560
PleI	GAGTC	5 cut(s)	126, 167, 1458, 1547, 2260
PluTI	GGCGCC	1 cut(s)	690
PmaCI	CACGTG	1 cut(s)	955
PmlI	CACGTG	1 cut(s)	955
PpsI	GAGTC	5 cut(s)	126, 167, 1458, 1547, 2260
Ppu21I	YACGTR	1 cut(s)	955
PshBI	ATTAAT	1 cut(s)	2516
PsiI	TTATAA	2 cut(s)	2175, 2603
Psp1406I	AACGTT	1 cut(s)	1503
Psp6I	CCWGG	1 cut(s)	2402
PspCI	CACGTG	1 cut(s)	955
PspFI	CCCAGC	1 cut(s)	2539
PspGI	CCWGG	1 cut(s)	2402
PspN4I	GGNNCC	4 cut(s)	166, 305, 688, 2017
PspPI	GGNCC	2 cut(s)	810, 873
PsrI	GAACNNNNNNTAC	2 cut(s)	477, 509
PstI	CTGCAG	1 cut(s)	1098
PstNI	CAGNNNCTG	3 cut(s)	1319, 1546, 1684
PsuI	RGATCY	2 cut(s)	1561, 1798
PvuII	CAGCTG	1 cut(s)	2369
RsaI	GTAC	5 cut(s)	112, 1073, 1474, 1830, 2064
RsaNI	GTAC	5 cut(s)	111, 1072, 1473, 1829, 2063
RseI	CAYNNNNRTG	2 cut(s)	54, 2250
SalI	GTCGAC	1 cut(s)	368
SaqAI	TTAA	8 cut(s)	66, 192, 900, 1484, 1955, 2229, 2516, 2631
Sau3AI	GATC	8 cut(s)	223, 430, 613, 894, 1207, 1351, 1561, 1798
Sau96I	GGNCC	2 cut(s)	810, 873
SchI	GAGTC	5 cut(s)	127, 168, 1459, 1547, 2260
ScrFI	CCNGG	1 cut(s)	2404
SduI	GDGCHC	2 cut(s)	325, 2131
SfaNI	GCATC	7 cut(s)	293, 632, 1552, 1684, 1757, 2149, 2405
SfcI	CTRYAG	3 cut(s)	232, 489, 1094
SfoI	GGCGCC	1 cut(s)	688
Sfr274I	CTCGAG	1 cut(s)	1784
SfuI	TTCGAA	1 cut(s)	1435
SinI	GGWCC	1 cut(s)	810
SlaI	CTCGAG	1 cut(s)	1784
SmiMI	CAYNNNNRTG	2 cut(s)	54, 2250
SmlI	CTYRAG	2 cut(s)	1784, 2630
SmoI	CTYRAG	2 cut(s)	1784, 2630
Sse9I	AATT	9 cut(s)	546, 924, 1107, 1454, 2066, 2335, 2513, 2517, 2523
SseBI	AGGCCT	1 cut(s)	1092
SspDI	GGCGCC	1 cut(s)	686
SspI	AATATT	1 cut(s)	1766
SspMI	CTAG	4 cut(s)	180, 254, 422, 914
StuI	AGGCCT	1 cut(s)	1092
StyD4I	CCNGG	1 cut(s)	2402
StyI	CCWWGG	2 cut(s)	1125, 1192
TaaI	ACNGT	8 cut(s)	128, 493, 1290, 1422, 1432, 1472, 1724, 1915
TaiI	ACGT	3 cut(s)	348, 957, 1506
TasI	AATT	9 cut(s)	546, 924, 1107, 1454, 2066, 2335, 2513, 2517, 2523
TatI	WGTACW	1 cut(s)	2062
TauI	GCSGC	3 cut(s)	89, 154, 2296
TfiI	GAWTC	7 cut(s)	637, 1082, 1115, 1814, 2501, 2544, 2560
Tru1I	TTAA	8 cut(s)	66, 192, 900, 1484, 1955, 2229, 2516, 2631
Tru9I	TTAA	8 cut(s)	66, 192, 900, 1484, 1955, 2229, 2516, 2631
TscAI	CASTG	5 cut(s)	1035, 1572, 2193, 2257, 2464
TseFI	GTSAC	4 cut(s)	185, 955, 1309, 2449
Tsp45I	GTSAC	4 cut(s)	185, 955, 1309, 2449
TspGWI	ACGGA	3 cut(s)	1998, 2028, 2338
TspRI	CASTG	5 cut(s)	1035, 1572, 2193, 2257, 2464
Vha464I	CTTAAG	1 cut(s)	2630
VpaK11BI	GGWCC	1 cut(s)	810
VspI	ATTAAT	1 cut(s)	2516
XagI	CCTNNNNNAGG	1 cut(s)	1124
XapI	RAATTY	1 cut(s)	546
XhoI	CTCGAG	1 cut(s)	1784
XmiI	GTMKAC	1 cut(s)	369
XmnI	GAANNNNTTC	1 cut(s)	1081
XspI	CTAG	4 cut(s)	180, 254, 422, 914

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.