Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0009795

Physical Location & Seq

Genomic Coordinates Forward (+)

22984420 .. 22989647

Gene Structure 5228 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g29700.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2619 bp

ATGTTGCGAGATTCAGTGGCAATGTGTGGCCAAGAGCTGGAGCAAATGTCCAGTGTTGATCAGGGCAGTCAGTATAGCCTCTCCGATGGCATTCTACCTTCTCTTGGTGCTGCCTCTCGCAACCACCGCAGACCTAAACTACGTCGTTTCATCATCTCCCCTTTTAATCCTCGTTACAAGCTTTGGCAGTCGTTTCTGATATTGTGGGTGTTCTACACGGCATGGGTGTGTCCCTTTGAGTTTGGGTTCCTGGACAGCCCTAGAGGACCTCTGGCTATTACTGATAATGTTGTGAATGGCCTATTTGGCATTGATATTATATTGACGTTCTTTGTGGCTTACCTTGATAAAACTACTTATTTGCTCATTGATTCCCCAAAGCTTATTGCTCTGAGATATGCCAAGACCTGGTTGGCCTTTGATATCATCTCCATCATCCCTTCAGAATTTGCTCAGACGGTTTTGCCTCATCCTCTTGCCAATTATGGATACTTCAATATTCTTCGGCTATGGCGTCTCCGAAGAGTCAGTGCAATGTTTGCCAGATTGGAGAAAGACCGGAATTTTAGCTACTTCTGGGTTCGATGCAGCAAGCTTGTTTTTGTTACTCTGTTTACCGTTCATTGTGGAGGCTGCATCTTCTACTTCATTGCTGCGAATTATCATGATCCTAAAAGGACATGGCTTGGGCTTCTGACAGACAACTTCAAGGATATAAGTGTGTGGAACCGGTACGTTACATCAATGTACTGGTCCATAATCACGCTCACCACCACCGGCTATGGTGATTTGCATCCTGTAAATTCTCAAGAAATGATTTTCGACATCTTCTATATGCTCTTTAATGTTGGACTGCAAGCTTACCTGATTGGAAACATGACCAACTTAATCGTCCATGGGACTGCTCGAACTAGACAGTTTAGAGATAGTATTCACGCTGCCTCGAGTTTTGCAGTAAGGAACCAAATACCTGATCGCCTTCACGAACAGATGCTTGCACATTTGTGTTTGAAGTACAGGACTAATTCAGAAGGGCTGCAGCAGCAAGAGACTCTTGATGCACTCCCTCACGCTATTAGATCAAGTATCTCACACTATCTCTTCAATTCACTAGTTGACAATGTTTACTTATTCCAGGGGGTATCAAGAGACATGCTTTTCCAACTGGTCGCAGAGATGAAGGCCGAGTATTTTCCTCCAAAAGAAGATGTCATTTTGCAGAATGAAGCACCTACTGATATGTATATCGTGGTAACTGGTTCTGTGGAACTTATATTGCAAAAGAATGGAGTAGAAATGGTGTTTCGTGAAGCAAAGACAGGAGATGTTTTTGGTGCCATAGGTGTTCTGTGTTACAGGCCCCAACTGTTCACAGTTCGGACTAAAGGACTTTGTCAGCTGTTGCGTTTGAACAGGACAAATTTCTTGAATATTGTTCAGGCAAATGTTGGAGATGGGACAATTATTATGAGCAATTTTCTTCACCATTTGAGAGATGAGAAGGATCCCATAATGGAAGGAATTTTAACGGATGTAGAACATATGCTGGCTCGGGGAAAATCGGGTATGCCTCTTAGTTTAGGGTTTGCTGCAAAGAGGAGTGATGATCTGTTGCTACATCAACTGCTAAGACGGGGTGCAGATCCGAATGAGACAGACCATAATGGCCGGACAGCTATGCACATTGCAGCATCAATTGGAAGCAAGCATTGTGTAACTTTACTTCTAGAGTACGGAGGTGATCCTAACATCAAAGATTGTGAAGAAAATGTTCCTCTATGGGAAGCAATAATAGGGAAGCATGAATCTTTGATTAAACTTCTCTTAGAAAATGGAGCTGAGATTAGTGCTGGCGGTGTGGGACATTATGCTTGTACTGCGGTTGAGCAAAACAGCTTAGAGCTGCTCAAAGACATTGTCCAATATGGTGGTGATGTTACACTTCCCAAGAGCGATGGGACTACAGCACTCCATACAGCAATAAGCGATGGAAATGCTGAGATAGTTAACTTCCTCTTAGAACAAGGAGCTGATCCTGATGAGAGGGATAGCTATGGATGGAGTCCGAGGGCTTTGGCAGAACATCAGGGACATGAAGAAATAATTGAGCTATTTCAGAACAAATCTGGAGTTAAGAAACCGACTACTGCTTCTCAGCCTAACGATCCACACCAACCACGCCTTGTGAAGTTCCACAGTGAGCCCACAATACCACCCTATGTCCGGGATGGCAGGCCAAACTCACAAACACAGTGGTTTGATGATACTCGGATAAGAAGGGTCAACAACTTTCCCAATTCGCTATTTGGGATCGTGTCAGCTGCCAATAACGGTGAGAGAGACAAAGACCCTATCAAACCTGCAGCCAATTTTGTTCGTGGTGGCCGTAGTAAGAGCAAATATCCAGCCAGAGTTACTATTAGCTGTCAAGAAAAAAGTGGAACTTCTTCAAAGCTTGTGGTTTTGCCAGAGTCCCTTGAACAACTGCTTGAAATTGGTGCCAAAAAATTTCAGTTTTGTCCCACTAAAGTTTTAACCACAGAAGGTGCTGAAGTTGAAGATATAGAGCTTGTTAGGGATGGTGATCATCTTGTTCTTGTCGGTGATGGTGAGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

873

Amino Acids

98.24

Weight (kDa)

6.25

Isoelectric Point (pI)

39.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	3 - 247	1.7e-41	Ion transport protein
Ion_trans_2	PF07885	149 - 240	1.1e-11	Ion channel
cNMP_binding	PF00027	340 - 425	6.2e-15	Cyclic nucleotide-binding domain
Ank_2	PF12796	471 - 559	8e-16	Ankyrin repeats (3 copies)
Ank_4	PF13637	473 - 519	2.7e-08	Ankyrin repeats (many copies)
Ank	PF00023	498 - 529	8.1e-07	Ankyrin repeat
Ank_2	PF12796	569 - 650	1.7e-12	Ankyrin repeats (3 copies)
Ank_5	PF13857	586 - 636	1.2e-09	Ankyrin repeats (many copies)
Ank	PF00023	595 - 626	1.7e-07	Ankyrin repeat
KHA	PF11834	747 - 809	1.9e-22	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0009795)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25600 AT4G32500
fragaria_vesca	FvH4_2g29700 FvH4_2g29700 FvH4_2g29700
malus_domestica	MD08G1142800.v1.1 MD15G1119300.v1.1
prunus_persica	Prupe.1G472600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0298701
rosa_laevigata	RLG00000011492
rosa_multiflora	Rmu_co8298943.1_g000001 Rmu_sc0008099.1_g000002 Rmu_sc0008142.1_g000002 Rmu_sc0032030.1_g000001
rosa_roxburghii	Rroxscaffold_7G00169400
rosa_rugosa	Rorug06G0282700
rosa_samantha	Rh6AG393800 Rh6BG401600 Rh6CG407500 Rh6DG394100
rosa_wichuraiana	Rw6G034430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2368
AccB1I	GGYRCC	2 cut(s)	1334, 2498
AccB7I	CCANNNNNTGG	2 cut(s)	37, 408
AciI	CCGC	3 cut(s)	127, 1854, 1880
AclWI	GGATC	8 cut(s)	662, 1499, 1512, 1637, 1736, 2027, 2159, 2318
AcoI	YGGCCR	3 cut(s)	28, 1666, 2383
AcsI	RAATTY	6 cut(s)	446, 562, 802, 1420, 1521, 2507
AcuI	CTGAAG	2 cut(s)	426, 2571
AcyI	GRCGYC	1 cut(s)	514
AfaI	GTAC	5 cut(s)	734, 749, 1016, 1733, 1876
AfiI	CCNNNNNNNGG	4 cut(s)	37, 104, 408, 2223
AgeI	ACCGGT	1 cut(s)	729
AhlI	ACTAGT	1 cut(s)	1111
AjnI	CCWGG	3 cut(s)	249, 407, 1134
AleI	CACNNNNGTG	1 cut(s)	1003
Alw26I	GTCTC	5 cut(s)	521, 1043, 1143, 1646, 2334
AlwI	GGATC	8 cut(s)	662, 1499, 1512, 1637, 1736, 2027, 2159, 2318
Ama87I	CYCGRG	2 cut(s)	943, 1551
AoxI	GGCC	8 cut(s)	28, 298, 414, 1182, 1358, 1666, 2234, 2383
ApoI	RAATTY	6 cut(s)	446, 562, 802, 1420, 1521, 2507
ArsI	GACNNNNNNTTYG	2 cut(s)	1902, 1934
AsiGI	ACCGGT	1 cut(s)	729
Asp700I	GAANNNNTTC	2 cut(s)	1770, 2446
AspS9I	GGNCC	3 cut(s)	266, 753, 1359
AsuC2I	CCSGG	1 cut(s)	2225
AsuHPI	GGTGA	8 cut(s)	760, 797, 1475, 1751, 1943, 2345, 2594, 2615
AvaI	CYCGRG	2 cut(s)	943, 1551
AvaII	GGWCC	2 cut(s)	266, 753
BalI	TGGCCA	1 cut(s)	30
BamHI	GGATCC	1 cut(s)	1504
BanI	GGYRCC	2 cut(s)	1334, 2498
BanII	GRGCYC	1 cut(s)	2205
BarI	GAAGNNNNNNTAC	2 cut(s)	1707, 1739
BccI	CCATC	9 cut(s)	80, 440, 1448, 1949, 1982, 2052, 2222, 2573, 2600
BceAI	ACGGC	2 cut(s)	234, 2370
BcgI	CGANNNNNNTGC	2 cut(s)	1976, 2010
BciT130I	CCWGG	3 cut(s)	251, 409, 1136
BciVI	GTATCC	1 cut(s)	482
BclI	TGATCA	2 cut(s)	58, 2584
BcnI	CCSGG	1 cut(s)	2225
BcoDI	GTCTC	5 cut(s)	521, 1043, 1143, 1646, 2334
BcuI	ACTAGT	1 cut(s)	1111
BfaI	CTAG	4 cut(s)	261, 912, 1112, 1727
BfmI	CTRYAG	3 cut(s)	1037, 1962, 2361
BfuAI	ACCTGC	1 cut(s)	2368
BfuI	GTATCC	1 cut(s)	482
BglI	GCCNNNNNGGC	1 cut(s)	306
Bme1390I	CCNGG	4 cut(s)	251, 409, 1136, 2225
Bme18I	GGWCC	2 cut(s)	266, 753
BmeT110I	CYCGRG	2 cut(s)	943, 1551
BmgT120I	GGNCC	3 cut(s)	266, 753, 1359
BmiI	GGNNCC	7 cut(s)	248, 728, 962, 1336, 1361, 1506, 2500
BmrFI	CCNGG	4 cut(s)	251, 409, 1136, 2225
BmsI	GCATC	6 cut(s)	575, 645, 802, 981, 1048, 1700
BpmI	CTGGAG	2 cut(s)	59, 2148
BpuEI	CTTGAG	1 cut(s)	792
BpuMI	CCSGG	1 cut(s)	2225
BsaBI	GATNNNNATC	2 cut(s)	792, 2583
BsaHI	GRCGYC	1 cut(s)	514
BsaJI	CCNNGG	3 cut(s)	895, 1135, 2066
BsaWI	WCCGGW	2 cut(s)	558, 729
BsaXI	ACNNNNNCTCC	2 cut(s)	32, 62
Bsc4I	CCNNNNNNNGG	4 cut(s)	37, 104, 408, 2223
Bse118I	RCCGGY	2 cut(s)	729, 776
Bse1I	ACTGG	4 cut(s)	51, 755, 1170, 1261
Bse3DI	GCAATG	4 cut(s)	27, 540, 648, 1683
Bse8I	GATNNNNATC	2 cut(s)	792, 2583
BseBI	CCWGG	3 cut(s)	251, 409, 1136
BseDI	CCNNGG	3 cut(s)	895, 1135, 2066
BseGI	GGATG	7 cut(s)	435, 469, 793, 1537, 2063, 2233, 2584
BseJI	GATNNNNATC	2 cut(s)	792, 2583
BseLI	CCNNNNNNNGG	4 cut(s)	37, 104, 408, 2223
BseMI	GCAATG	4 cut(s)	27, 540, 648, 1683
BseMII	CTCAG	5 cut(s)	383, 467, 1830, 1989, 2168
BseNI	ACTGG	4 cut(s)	51, 755, 1170, 1261
BseRI	GAGGAG	1 cut(s)	1612
BsgI	GTGCAG	1 cut(s)	1659
BshFI	GGCC	8 cut(s)	30, 300, 416, 1184, 1360, 1668, 2236, 2385
BshNI	GGYRCC	2 cut(s)	1334, 2498
BshTI	ACCGGT	1 cut(s)	729
BsiHKCI	CYCGRG	2 cut(s)	943, 1551
BsiSI	CCGG	5 cut(s)	559, 730, 777, 1669, 2224
BslFI	GGGAC	8 cut(s)	216, 913, 1471, 1875, 1972, 2103, 2458, 2505
BslI	CCNNNNNNNGG	4 cut(s)	37, 104, 408, 2223
BsmAI	GTCTC	5 cut(s)	521, 1043, 1143, 1646, 2334
BsmBI	CGTCTC	1 cut(s)	521
BsmFI	GGGAC	8 cut(s)	216, 913, 1471, 1875, 1972, 2103, 2458, 2505
BsmI	GAATGC	1 cut(s)	90
BsnI	GGCC	8 cut(s)	30, 300, 416, 1184, 1360, 1668, 2236, 2385
BsoBI	CYCGRG	2 cut(s)	943, 1551
Bsp1286I	GDGCHC	1 cut(s)	2205
Bsp19I	CCATGG	1 cut(s)	895
BspACI	CCGC	3 cut(s)	127, 1854, 1880
BspANI	GGCC	8 cut(s)	30, 300, 416, 1184, 1360, 1668, 2236, 2385
BspCNI	CTCAG	5 cut(s)	384, 466, 1831, 1990, 2167
BspHI	TCATGA	1 cut(s)	664
BspLI	GGNNCC	7 cut(s)	248, 728, 962, 1336, 1361, 1506, 2500
BspMAI	CTGCAG	2 cut(s)	1041, 2365
BspMI	ACCTGC	1 cut(s)	2368
BspPI	GGATC	8 cut(s)	662, 1499, 1512, 1637, 1736, 2027, 2159, 2318
BspT107I	GGYRCC	2 cut(s)	1334, 2498
BsrDI	GCAATG	4 cut(s)	27, 540, 648, 1683
BsrFI	RCCGGY	2 cut(s)	729, 776
BsrI	ACTGG	4 cut(s)	51, 755, 1170, 1261
BssAI	RCCGGY	2 cut(s)	729, 776
BssECI	CCNNGG	3 cut(s)	895, 1135, 2066
BssNI	GRCGYC	1 cut(s)	514
BssT1I	CCWWGG	1 cut(s)	895
Bst2UI	CCWGG	3 cut(s)	251, 409, 1136
Bst4CI	ACNGT	8 cut(s)	460, 619, 918, 1368, 1375, 2198, 2253, 2333
Bst6I	CTCTTC	2 cut(s)	517, 1106
BstACI	GRCGYC	1 cut(s)	514
BstAPI	GCANNNNNTGC	1 cut(s)	539
BstC8I	GCNNGC	7 cut(s)	593, 858, 996, 1548, 1706, 1852, 2234
BstDSI	CCRYGG	1 cut(s)	895
BstF5I	GGATG	7 cut(s)	435, 469, 793, 1537, 2063, 2233, 2584
BstMAI	GTCTC	5 cut(s)	521, 1043, 1143, 1646, 2334
BstMWI	GCNNNNNNNGC	5 cut(s)	126, 306, 539, 1042, 1877
BstNI	CCWGG	3 cut(s)	251, 409, 1136
BstNSI	RCATGY	1 cut(s)	1156
BstSCI	CCNGG	4 cut(s)	249, 407, 1134, 2223
BstSFI	CTRYAG	3 cut(s)	1037, 1962, 2361
BstX2I	RGATCY	2 cut(s)	1504, 1642
BstXI	CCANNNNNNTGG	1 cut(s)	1928
BstYI	RGATCY	2 cut(s)	1504, 1642
BsuI	GTATCC	1 cut(s)	482
BsuRI	GGCC	8 cut(s)	30, 300, 416, 1184, 1360, 1668, 2236, 2385
BtgI	CCRYGG	1 cut(s)	895
BtgZI	GCGATG	2 cut(s)	1968, 2001
BtsCI	GGATG	7 cut(s)	435, 469, 793, 1537, 2063, 2233, 2584
BtsIMutI	CAGTG	5 cut(s)	21, 58, 535, 2203, 2258
BveI	ACCTGC	1 cut(s)	2368
Cac8I	GCNNGC	7 cut(s)	593, 858, 996, 1548, 1706, 1852, 2234
CciI	TCATGA	1 cut(s)	664
Cfr10I	RCCGGY	2 cut(s)	729, 776
Cfr13I	GGNCC	3 cut(s)	266, 753, 1359
CseI	GACGC	1 cut(s)	503
CsiI	ACCWGGT	1 cut(s)	407
Csp6I	GTAC	5 cut(s)	733, 748, 1015, 1732, 1875
CspAI	ACCGGT	1 cut(s)	729
CspCI	CAANNNNNGTGG	4 cut(s)	2234, 2269, 2439, 2474
CviAII	CATG	8 cut(s)	222, 665, 681, 877, 896, 1153, 1802, 2093
CviQI	GTAC	5 cut(s)	733, 748, 1015, 1732, 1875
EaeI	YGGCCR	3 cut(s)	28, 1666, 2383
Eam1104I	CTCTTC	2 cut(s)	517, 1106
EarI	CTCTTC	2 cut(s)	517, 1106
Eco130I	CCWWGG	1 cut(s)	895
Eco24I	GRGCYC	1 cut(s)	2205
Eco32I	GATATC	1 cut(s)	424
Eco47I	GGWCC	2 cut(s)	266, 753
Eco57I	CTGAAG	2 cut(s)	426, 2571
Eco88I	CYCGRG	2 cut(s)	943, 1551
EcoO109I	RGGNCCY	2 cut(s)	266, 1359
EcoRII	CCWGG	3 cut(s)	249, 407, 1134
EcoRV	GATATC	1 cut(s)	424
EcoT14I	CCWWGG	1 cut(s)	895
EcoT38I	GRGCYC	1 cut(s)	2205
ErhI	CCWWGG	1 cut(s)	895
Esp3I	CGTCTC	1 cut(s)	521
FaeI	CATG	8 cut(s)	225, 668, 684, 880, 899, 1156, 1805, 2096
FalI	AAGNNNNNCTT	4 cut(s)	1038, 1070, 2428, 2460
FaqI	GGGAC	8 cut(s)	216, 913, 1471, 1875, 1972, 2103, 2458, 2505
FatI	CATG	8 cut(s)	221, 664, 680, 876, 895, 1152, 1801, 2092
FauNDI	CATATG	1 cut(s)	1542
FbaI	TGATCA	2 cut(s)	58, 2584
FokI	GGATG	7 cut(s)	422, 456, 780, 1544, 2070, 2240, 2591
FriOI	GRGCYC	1 cut(s)	2205
FspBI	CTAG	4 cut(s)	261, 912, 1112, 1727
GsuI	CTGGAG	2 cut(s)	59, 2148
HaeIII	GGCC	8 cut(s)	30, 300, 416, 1184, 1360, 1668, 2236, 2385
HapII	CCGG	5 cut(s)	559, 730, 777, 1669, 2224
HgaI	GACGC	1 cut(s)	503
Hin1I	GRCGYC	1 cut(s)	514
Hin1II	CATG	8 cut(s)	225, 668, 684, 880, 899, 1156, 1805, 2096
HincII	GTYRAC	3 cut(s)	1117, 2008, 2284
HindII	GTYRAC	3 cut(s)	1117, 2008, 2284
HindIII	AAGCTT	5 cut(s)	179, 380, 593, 858, 2453
HinfI	GANTC	7 cut(s)	11, 371, 525, 1051, 1805, 2062, 2471
HpaI	GTTAAC	1 cut(s)	2008
HpaII	CCGG	5 cut(s)	559, 730, 777, 1669, 2224
HphI	GGTGA	8 cut(s)	760, 797, 1475, 1751, 1943, 2345, 2594, 2615
Hpy166II	GTNNAC	6 cut(s)	615, 1117, 1126, 1371, 2008, 2284
Hpy8I	GTNNAC	6 cut(s)	615, 1117, 1126, 1371, 2008, 2284
Hpy99I	CGWCG	1 cut(s)	147
HpyAV	CCTTC	9 cut(s)	108, 450, 989, 1025, 1174, 1495, 1511, 2271, 2537
HpyCH4III	ACNGT	8 cut(s)	460, 619, 918, 1368, 1375, 2198, 2253, 2333
HpyCH4IV	ACGT	3 cut(s)	142, 326, 735
HpyF10VI	GCNNNNNNNGC	5 cut(s)	126, 306, 539, 1042, 1877
HpySE526I	ACGT	3 cut(s)	142, 326, 735
Hsp92I	GRCGYC	1 cut(s)	514
Hsp92II	CATG	8 cut(s)	225, 668, 684, 880, 899, 1156, 1805, 2096
Ksp22I	TGATCA	2 cut(s)	58, 2584
KspAI	GTTAAC	1 cut(s)	2008
LmnI	GCTCC	3 cut(s)	40, 1835, 2027
LweI	GCATC	6 cut(s)	575, 645, 802, 981, 1048, 1700
MabI	ACCWGGT	1 cut(s)	407
MaeI	CTAG	4 cut(s)	261, 912, 1112, 1727
MaeII	ACGT	3 cut(s)	142, 326, 735
MaeIII	GTNAC	8 cut(s)	173, 604, 736, 1252, 1352, 1714, 1936, 2413
MfeI	CAATTG	1 cut(s)	1695
MflI	RGATCY	2 cut(s)	1504, 1642
MhlI	GDGCHC	1 cut(s)	2205
MlsI	TGGCCA	1 cut(s)	30
MluNI	TGGCCA	1 cut(s)	30
MlyI	GAGTC	4 cut(s)	534, 1045, 2071, 2480
MmeI	TCCRAC	3 cut(s)	829, 1186, 1429
Mox20I	TGGCCA	1 cut(s)	30
MroXI	GAANNNNTTC	2 cut(s)	1770, 2446
MscI	TGGCCA	1 cut(s)	30
MseI	TTAA	8 cut(s)	165, 843, 887, 1526, 1815, 2007, 2133, 2534
MslI	CAYNNNNRTG	2 cut(s)	226, 1003
Msp20I	TGGCCA	1 cut(s)	30
MspA1I	CMGCKG	2 cut(s)	1399, 2321
MspI	CCGG	5 cut(s)	559, 730, 777, 1669, 2224
MspR9I	CCNGG	4 cut(s)	251, 409, 1136, 2225
MunI	CAATTG	1 cut(s)	1695
Mva1269I	GAATGC	1 cut(s)	90
MvaI	CCWGG	3 cut(s)	251, 409, 1136
MwoI	GCNNNNNNNGC	5 cut(s)	126, 306, 539, 1042, 1877
NciI	CCSGG	1 cut(s)	2225
NcoI	CCATGG	1 cut(s)	895
NdeI	CATATG	1 cut(s)	1542
NlaIII	CATG	8 cut(s)	225, 668, 684, 880, 899, 1156, 1805, 2096
NlaIV	GGNNCC	7 cut(s)	248, 728, 962, 1336, 1361, 1506, 2500
NmeAIII	GCCGAG	1 cut(s)	1210
NspI	RCATGY	1 cut(s)	1156
OliI	CACNNNNGTG	1 cut(s)	1003
PaeR7I	CTCGAG	1 cut(s)	943
PagI	TCATGA	1 cut(s)	664
PcsI	WCGNNNNNNNCGW	2 cut(s)	511, 2383
PctI	GAATGC	1 cut(s)	90
PdmI	GAANNNNTTC	2 cut(s)	1770, 2446
PfeI	GAWTC	3 cut(s)	11, 371, 1805
PflFI	GACNNNGTC	2 cut(s)	1392, 1916
PflMI	CCANNNNNTGG	2 cut(s)	37, 408
PfoI	TCCNGGA	2 cut(s)	249, 2223
PinAI	ACCGGT	1 cut(s)	729
PleI	GAGTC	4 cut(s)	533, 1045, 2070, 2479
PpsI	GAGTC	4 cut(s)	533, 1045, 2070, 2479
PpuMI	RGGWCCY	1 cut(s)	266
Psp5II	RGGWCCY	1 cut(s)	266
Psp6I	CCWGG	3 cut(s)	249, 407, 1134
PspGI	CCWGG	3 cut(s)	249, 407, 1134
PspN4I	GGNNCC	7 cut(s)	248, 728, 962, 1336, 1361, 1506, 2500
PspPI	GGNCC	3 cut(s)	266, 753, 1359
PspPPI	RGGWCCY	1 cut(s)	266
PspXI	VCTCGAGB	1 cut(s)	943
PstI	CTGCAG	2 cut(s)	1041, 2365
PsuI	RGATCY	2 cut(s)	1504, 1642
PsyI	GACNNNGTC	2 cut(s)	1392, 1916
PvuII	CAGCTG	2 cut(s)	1399, 2321
RsaI	GTAC	5 cut(s)	734, 749, 1016, 1733, 1876
RsaNI	GTAC	5 cut(s)	733, 748, 1015, 1732, 1875
RseI	CAYNNNNRTG	2 cut(s)	226, 1003
SaqAI	TTAA	8 cut(s)	165, 843, 887, 1526, 1815, 2007, 2133, 2534
Sau96I	GGNCC	3 cut(s)	266, 753, 1359
SchI	GAGTC	4 cut(s)	534, 1045, 2071, 2480
ScrFI	CCNGG	4 cut(s)	251, 409, 1136, 2225
SduI	GDGCHC	1 cut(s)	2205
SexAI	ACCWGGT	1 cut(s)	407
SfaNI	GCATC	6 cut(s)	575, 645, 802, 981, 1048, 1700
SfcI	CTRYAG	3 cut(s)	1037, 1962, 2361
Sfr274I	CTCGAG	1 cut(s)	943
SinI	GGWCC	2 cut(s)	266, 753
SlaI	CTCGAG	1 cut(s)	943
SmiMI	CAYNNNNRTG	2 cut(s)	226, 1003
SmlI	CTYRAG	2 cut(s)	807, 943
SmoI	CTYRAG	2 cut(s)	807, 943
SpeI	ACTAGT	1 cut(s)	1111
SsiI	CCGC	3 cut(s)	127, 1854, 1880
SspI	AATATT	2 cut(s)	499, 1432
SspMI	CTAG	4 cut(s)	261, 912, 1112, 1727
StyD4I	CCNGG	4 cut(s)	249, 407, 1134, 2223
StyI	CCWWGG	1 cut(s)	895
TaaI	ACNGT	8 cut(s)	460, 619, 918, 1368, 1375, 2198, 2253, 2333
TaiI	ACGT	3 cut(s)	145, 329, 738
TaqI	TCGA	4 cut(s)	583, 822, 907, 944
TatI	WGTACW	3 cut(s)	747, 1014, 1874
TfiI	GAWTC	3 cut(s)	11, 371, 1805
Tru1I	TTAA	8 cut(s)	165, 843, 887, 1526, 1815, 2007, 2133, 2534
Tru9I	TTAA	8 cut(s)	165, 843, 887, 1526, 1815, 2007, 2133, 2534
TscAI	CASTG	5 cut(s)	21, 58, 535, 2203, 2258
TspDTI	ATGAA	7 cut(s)	139, 611, 637, 1193, 1239, 1818, 2109
TspGWI	ACGGA	2 cut(s)	1544, 1749
TspRI	CASTG	5 cut(s)	21, 58, 535, 2203, 2258
Tth111I	GACNNNGTC	2 cut(s)	1392, 1916
Van91I	CCANNNNNTGG	2 cut(s)	37, 408
VpaK11BI	GGWCC	2 cut(s)	266, 753
XapI	RAATTY	6 cut(s)	446, 562, 802, 1420, 1521, 2507
XbaI	TCTAGA	1 cut(s)	1726
XceI	RCATGY	1 cut(s)	1156
XcmI	CCANNNNNNNNNTGG	1 cut(s)	409
XhoI	CTCGAG	1 cut(s)	943
XmnI	GAANNNNTTC	2 cut(s)	1770, 2446
XspI	CTAG	4 cut(s)	261, 912, 1112, 1727

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.