Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009795

Physical Location & Seq

Genomic Coordinates Reverse (-)

14166404 .. 14171610

Gene Structure 5207 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1142800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2673 bp

ATGATGAGGAAGGGGTCAATGGCAATGTCGATGTCAATGTGCGGGCAGGGGTGTGGTGGAGGAAAACAGGAGAAGACGGAAGAAATGTCGAGGGACGGCAGCCATGCCAGCCAGTTTAACCTCATCGATGGCATTTTGCCGTCTCTTGGCGCCACTGCTCCAAACCGTCCCAAACACAAGTTCCGCCGTTTCATCATCTCCCCATTCAATTATTATTACAAACTTTGGCAGTCATTTCTTATACTCCTGGTTTTCTACACTGCTTGGGTGTGTCCCTTTGAATTTGGATTCTTGGACAGAGCAAAAGGACTTATTGCCATAGCTGATAATGTTGTCAATGCACTTTTCGCTATCGACATTGTATTGACATTCTTTGTGGCCTACCTTGATAAAACTACTTATCTCCTCATTGACAACCACAAGCTCATCGCTCTGAGATATGCCAAAACATGGCTGGCCTTTGACATCATCTCCACAATCCCTTCGGAACTTGCTCAGCGTGTTCTGCCTCCCCCTCTCGAGACTTATGGATACTTCAATATTCTCCGCCTTTGGCGTCTCCGAAGAGTCAGTGCAATGATTTCCAGATTGGAAAAGGACAGGAATTATAGCTACTTTTGGGTTCGATACTGCAAGCTTATTTTCGTTACACTCCTCACAGTTCATTGCGCAGGCTGCTTCTTCTATTTCCTTGCTGCGCATTATCGTAACCCAAGAAGGACATGGTTTGGGCTTATCGAAGAAAACTTTCAACAATTACCTTTATGGGACCGGTATGTGAGCTCAATGTACTGGTCCATAATCACGCTCACAACCACCGGCTATGGGGATTTTCATCCTGTAAATTCACAGGAGATGACATTCGATATCTTCTATATGCTCTTCAATCTTGGGCTGCAAGCTTACCTGATCGGTAATATGACCAACTTGATTGTCCATGGAACTGCTCGAACCAGACAATTTAGAGACACTATTCAAGCTGCAACAAGTTTTGCGCGTAGGAACCAAATACCTGATCACCTGCACGAGCAGATGCTTGCTCATTTGTGTTTGAAGTACAGAACTAATTCAGAAGGGCTGCAGCAGCAAGAGGCTCTCGATGCGCTCCCTAAGGCCATTCGATCTAGCATCTCTCACTATCTCTTCTATCCACTTCTTGATAGTGTCTACTTGTTCCAAGGTGTATCAAGAGATCTACTTTTCCAATTGGTCTCTGAGATGAAGGCTGAATATTTTCCTCCAAAAGAAGTTGTAATCTTGCAGAATGAAGCACCCACTGACATGTATATCGTGGTTACCGGCTCTGTGGAACTTATTATGCATAGGAATGGAGCAACACCGGTTGTTCGTGAAGCCAAGACAGGAGACGTTGTTGGTGAAATAGGTGTGCTTTGTTACAGGCCTCAAATGGTCACAGTTAGGACGAAAGGACTCTCCCAGCTTCTGCGTTTGAACCGTACAGCTTTCTTAAACCTTGTTCAAGCAAATGTTGGAGATGGAACGATAATCATGAACAATTTTCTTAAGCATTTGAAAGAAATGAAGAATCCGCTGGCAGAAGGAATTTTGAGGGACATAGAGCATGTGCTGGCTAGCGGTAGAATGGATCTGCCTCTTAGTTTATGCTTTGCTGCACAGAGGGGTGATGATATGTTGCTGCATCAATTGCTCAGGCGGGGTTCAGATCCAAATGAGATGGATGATAATGGACGAACAGCTATGCACATTGCAGCATCTAATGGAAATGAACATTGTGCCCTTTTACTTCTAGAGTATGAAGCCGATCCCAATATACAAGATTCGGAAGGAAATGTTCCTCTATGGGAAGCAATATCAGGGAAGCATGAGTCTTTGATTAAACTTCTCTTAGACAATGGAGCGAAGATAAATTCTGGCAATGTGGGAAATTTTGCTTGTTCTGCTGTTGAGCAAAACAACTTGGAGCTGCTTAAATACATTGTCAAGTATAACGGTGATGTAACACTGCCCAAGAACAATGGGACTACAGCACTTCACACGGCAGTATGCGAAGAAAACGTAGAGATAGCTAAGTTCCTCTTGGAACAAGGAGCTGAGGCTGACAAGCCAGATAGCTACGGATGGACTCCTAGGGCTTTGGCAGAGCATCAGGGACATGACAAAATAATGGAACTGTTTCAGCAAAAATCGGAGAGTAAGAAACCAAGCAAGAAACAAACTGTTGTTCCTATGCCAGAGGATCCGATACTATCACGTCCTGGGAAGTTCAGAAGTGAGCCTACATTACCTCCCTACGCACGGGATGGCAGGCCATTCAACTCAGATATGACATCTGAAAATACTCTTAGAATGGATAAAAGCTTTCGCAATTCACTGTTCGGTTTCATGTCAACCGCCAATACTAATACCGGTGAGAAAGATAGAGACAATGTCACATCTACAGGCAGTTTGACAAGGAGCACAAGTAGGAACAGCTATCCAGCCAGAGTGACACTTAGCTGCCCGGAAAAAGGCAAAACTGCTGCAAAGCTTGTGCTTCTGCCTGACTCCCTTCAGCAACTACTTGACCTTGGTGCCAAGAAATTCCAGTTTTCAGCCACAAAAGTTTTAACCAAAGACAGAGCTGAAATTGAAGATATAGAGCTTGTTAGAGACGGCGATCATCTTGTTCTTGTTGGTGAGTCTGAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

891

Amino Acids

100.76

Weight (kDa)

7.86

Isoelectric Point (pI)

41.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	73 - 318	3.7e-40	Ion transport protein
Ion_trans_2	PF07885	219 - 311	1.6e-11	Ion channel
cNMP_binding	PF00027	410 - 496	2.9e-16	Cyclic nucleotide-binding domain
Ank_2	PF12796	541 - 631	8.7e-19	Ankyrin repeats (3 copies)
Ank_4	PF13637	541 - 590	2.8e-09	Ankyrin repeats (many copies)
Ank_5	PF13857	556 - 607	3.2e-06	Ankyrin repeats (many copies)
Ank	PF00023	569 - 600	2.6e-07	Ankyrin repeat
Ank_2	PF12796	641 - 721	1.8e-12	Ankyrin repeats (3 copies)
KHA	PF11834	822 - 884	1.2e-22	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0009795)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25600 AT4G32500
fragaria_vesca	FvH4_2g29700 FvH4_2g29700 FvH4_2g29700
malus_domestica	MD08G1142800.v1.1 MD15G1119300.v1.1
prunus_persica	Prupe.1G472600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0298701
rosa_laevigata	RLG00000011492
rosa_multiflora	Rmu_co8298943.1_g000001 Rmu_sc0008099.1_g000002 Rmu_sc0008142.1_g000002 Rmu_sc0032030.1_g000001
rosa_roxburghii	Rroxscaffold_7G00169400
rosa_rugosa	Rorug06G0282700
rosa_samantha	Rh6AG393800 Rh6BG401600 Rh6CG407500 Rh6DG394100
rosa_wichuraiana	Rw6G034430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1029
Acc16I	TGCGCA	2 cut(s)	670, 699
Acc36I	ACCTGC	1 cut(s)	1029
AccB1I	GGYRCC	2 cut(s)	149, 2552
AccB7I	CCANNNNNTGG	1 cut(s)	450
AccI	GTMKAC	1 cut(s)	1167
AccII	CGCG	1 cut(s)	997
AciI	CCGC	7 cut(s)	42, 184, 547, 1550, 1596, 1675, 2373
AclWI	GGATC	5 cut(s)	1614, 1679, 1778, 2213, 2226
AcsI	RAATTY	6 cut(s)	281, 844, 1563, 1888, 1906, 2561
AcuI	CTGAAG	1 cut(s)	2516
AcyI	GRCGYC	2 cut(s)	150, 556
AfaI	GTAC	3 cut(s)	791, 1058, 1459
AfiI	CCNNNNNNNGG	5 cut(s)	146, 450, 825, 2277, 2489
AflII	CTTAAG	1 cut(s)	1523
AflIII	ACRYGT	1 cut(s)	1281
AgeI	ACCGGT	3 cut(s)	771, 1339, 2387
AjiI	CACGTC	1 cut(s)	2234
AjnI	CCWGG	2 cut(s)	246, 2236
AjuI	GAANNNNNNNTTGG	2 cut(s)	164, 196
Alw21I	GWGCWC	2 cut(s)	785, 2441
Alw26I	GTCTC	8 cut(s)	147, 515, 563, 960, 1216, 1359, 2397, 2625
AlwI	GGATC	5 cut(s)	1614, 1679, 1778, 2213, 2226
AlwNI	CAGNNNCTG	1 cut(s)	1444
Ama87I	CYCGRG	1 cut(s)	518
AoxI	GGCC	5 cut(s)	378, 456, 1113, 1400, 2288
ApoI	RAATTY	6 cut(s)	281, 844, 1563, 1888, 1906, 2561
AsiGI	ACCGGT	3 cut(s)	771, 1339, 2387
Asp700I	GAANNNNTTC	4 cut(s)	747, 1066, 1233, 2154
AspA2I	CCTAGG	1 cut(s)	2108
AspLEI	GCGC	5 cut(s)	152, 671, 700, 997, 1105
AspS9I	GGNCC	2 cut(s)	769, 795
AsuC2I	CCSGG	1 cut(s)	2483
AsuHPI	GGTGA	6 cut(s)	1010, 1388, 1655, 1985, 2402, 2669
AsuNHI	GCTAGC	1 cut(s)	1592
AvaI	CYCGRG	1 cut(s)	518
AvaII	GGWCC	2 cut(s)	769, 795
AvrII	CCTAGG	1 cut(s)	2108
AxyI	CCTNAGG	1 cut(s)	1110
BaeGI	GKGCMC	1 cut(s)	1759
BamHI	GGATCC	1 cut(s)	2218
BanI	GGYRCC	2 cut(s)	149, 2552
BanII	GRGCYC	1 cut(s)	785
BarI	GAAGNNNNNNTAC	2 cut(s)	2242, 2274
BauI	CACGAG	1 cut(s)	1025
BbsI	GAAGAC	1 cut(s)	80
Bbv12I	GWGCWC	2 cut(s)	785, 2441
BbvCI	CCTCAGC	1 cut(s)	2073
BccI	CCATC	5 cut(s)	122, 1490, 1690, 2094, 2276
BceAI	ACGGC	5 cut(s)	112, 124, 171, 2034, 2650
BciT130I	CCWGG	2 cut(s)	248, 2238
BciVI	GTATCC	1 cut(s)	524
BclI	TGATCA	1 cut(s)	1015
BcnI	CCSGG	1 cut(s)	2483
BcoDI	GTCTC	8 cut(s)	147, 515, 563, 960, 1216, 1359, 2397, 2625
BfaI	CTAG	4 cut(s)	1125, 1593, 1769, 2109
BfmI	CTRYAG	3 cut(s)	1079, 2004, 2418
BfoI	RGCGCY	1 cut(s)	153
BfrI	CTTAAG	1 cut(s)	1523
BfuAI	ACCTGC	1 cut(s)	1029
BfuI	GTATCC	1 cut(s)	524
BglII	AGATCT	1 cut(s)	1192
BlnI	CCTAGG	1 cut(s)	2108
BlpI	GCTNAGC	1 cut(s)	495
Bme1390I	CCNGG	3 cut(s)	248, 2238, 2483
Bme18I	GGWCC	2 cut(s)	769, 795
BmeT110I	CYCGRG	1 cut(s)	518
BmgBI	CACGTC	1 cut(s)	2234
BmgT120I	GGNCC	2 cut(s)	769, 795
BmiI	GGNNCC	5 cut(s)	151, 770, 1004, 2220, 2554
BmrFI	CCNGG	3 cut(s)	248, 2238, 2483
BmsI	GCATC	6 cut(s)	1023, 1090, 1137, 1669, 1742, 2134
BmtI	GCTAGC	1 cut(s)	1596
BpiI	GAAGAC	1 cut(s)	80
Bpu10I	CCTNAGC	2 cut(s)	1670, 2073
Bpu1102I	GCTNAGC	1 cut(s)	495
BpuMI	CCSGG	1 cut(s)	2483
Bsa29I	ATCGAT	1 cut(s)	126
BsaBI	GATNNNNATC	1 cut(s)	834
BsaHI	GRCGYC	2 cut(s)	150, 556
BsaI	GGTCTC	1 cut(s)	1216
BsaJI	CCNNGG	5 cut(s)	937, 1177, 2108, 2237, 2548
BsaWI	WCCGGW	3 cut(s)	771, 1339, 2387
BsaXI	ACNNNNNCTCC	8 cut(s)	455, 485, 1356, 1386, 2251, 2281, 2428, 2458
Bsc4I	CCNNNNNNNGG	5 cut(s)	146, 450, 825, 2277, 2489
Bse118I	RCCGGY	5 cut(s)	771, 818, 1298, 1339, 2387
Bse1I	ACTGG	3 cut(s)	112, 797, 2566
Bse21I	CCTNAGG	1 cut(s)	1110
Bse3DI	GCAATG	5 cut(s)	30, 582, 664, 1725, 1903
Bse8I	GATNNNNATC	1 cut(s)	834
BseBI	CCWGG	2 cut(s)	248, 2238
BseCI	ATCGAT	1 cut(s)	126
BseDI	CCNNGG	5 cut(s)	937, 1177, 2108, 2237, 2548
BseGI	GGATG	4 cut(s)	835, 1705, 2105, 2287
BseJI	GATNNNNATC	1 cut(s)	834
BseLI	CCNNNNNNNGG	5 cut(s)	146, 450, 825, 2277, 2489
BseMI	GCAATG	5 cut(s)	30, 582, 664, 1725, 1903
BseMII	CTCAG	7 cut(s)	425, 509, 1206, 1684, 2064, 2313, 2655
BseNI	ACTGG	3 cut(s)	112, 797, 2566
BseRI	GAGGAG	2 cut(s)	395, 644
BseSI	GKGCMC	1 cut(s)	1759
BseYI	CCCAGC	1 cut(s)	1437
BsgI	GTGCAG	2 cut(s)	1007, 1617
Bsh1236I	CGCG	1 cut(s)	997
BshFI	GGCC	5 cut(s)	380, 458, 1115, 1402, 2290
BshNI	GGYRCC	2 cut(s)	149, 2552
BshTI	ACCGGT	3 cut(s)	771, 1339, 2387
BshVI	ATCGAT	1 cut(s)	126
BsiHKAI	GWGCWC	2 cut(s)	785, 2441
BsiHKCI	CYCGRG	1 cut(s)	518
BsiSI	CCGG	6 cut(s)	772, 819, 1299, 1340, 2388, 2483
BslFI	GGGAC	7 cut(s)	107, 153, 258, 782, 1586, 2014, 2145
BslI	CCNNNNNNNGG	5 cut(s)	146, 450, 825, 2277, 2489
BsmAI	GTCTC	8 cut(s)	147, 515, 563, 960, 1216, 1359, 2397, 2625
BsmBI	CGTCTC	4 cut(s)	147, 563, 1359, 2625
BsmFI	GGGAC	7 cut(s)	107, 153, 258, 782, 1586, 2014, 2145
BsnI	GGCC	5 cut(s)	380, 458, 1115, 1402, 2290
Bso31I	GGTCTC	1 cut(s)	1216
BsoBI	CYCGRG	1 cut(s)	518
Bsp1286I	GDGCHC	3 cut(s)	785, 1759, 2441
Bsp143I	GATC	9 cut(s)	909, 1015, 1121, 1192, 1606, 1684, 1783, 2218, 2638
Bsp1720I	GCTNAGC	1 cut(s)	495
Bsp19I	CCATGG	1 cut(s)	937
BspACI	CCGC	7 cut(s)	42, 184, 547, 1550, 1596, 1675, 2373
BspANI	GGCC	5 cut(s)	380, 458, 1115, 1402, 2290
BspCNI	CTCAG	7 cut(s)	426, 508, 1207, 1683, 2065, 2312, 2656
BspDI	ATCGAT	1 cut(s)	126
BspFNI	CGCG	1 cut(s)	997
BspHI	TCATGA	1 cut(s)	1509
BspLI	GGNNCC	5 cut(s)	151, 770, 1004, 2220, 2554
BspMAI	CTGCAG	1 cut(s)	1083
BspMI	ACCTGC	1 cut(s)	1029
BspOI	GCTAGC	1 cut(s)	1596
BspPI	GGATC	5 cut(s)	1614, 1679, 1778, 2213, 2226
BspQI	GCTCTTC	1 cut(s)	887
BspT107I	GGYRCC	2 cut(s)	149, 2552
BspTI	CTTAAG	1 cut(s)	1523
BspTNI	GGTCTC	1 cut(s)	1216
BsrDI	GCAATG	5 cut(s)	30, 582, 664, 1725, 1903
BsrFI	RCCGGY	5 cut(s)	771, 818, 1298, 1339, 2387
BsrI	ACTGG	3 cut(s)	112, 797, 2566
BssAI	RCCGGY	5 cut(s)	771, 818, 1298, 1339, 2387
BssECI	CCNNGG	5 cut(s)	937, 1177, 2108, 2237, 2548
BssMI	GATC	9 cut(s)	909, 1015, 1121, 1192, 1606, 1684, 1783, 2218, 2638
BssNI	GRCGYC	2 cut(s)	150, 556
BssSI	CACGAG	1 cut(s)	1025
BssT1I	CCWWGG	4 cut(s)	937, 1177, 2108, 2548
Bst2BI	CACGAG	1 cut(s)	1025
Bst2UI	CCWGG	2 cut(s)	248, 2238
Bst4CI	ACNGT	8 cut(s)	167, 661, 1417, 1457, 1973, 2154, 2200, 2355
Bst6I	CTCTTC	3 cut(s)	559, 887, 1148
BstACI	GRCGYC	2 cut(s)	150, 556
BstAFI	CTTAAG	1 cut(s)	1523
BstAPI	GCANNNNNTGC	1 cut(s)	1666
BstDSI	CCRYGG	1 cut(s)	937
BstEII	GGTNACC	1 cut(s)	1294
BstF5I	GGATG	4 cut(s)	835, 1705, 2105, 2287
BstFNI	CGCG	1 cut(s)	997
BstH2I	RGCGCY	1 cut(s)	153
BstHHI	GCGC	5 cut(s)	152, 671, 700, 997, 1105
BstKTI	GATC	9 cut(s)	912, 1018, 1124, 1195, 1609, 1687, 1786, 2221, 2641
BstMAI	GTCTC	8 cut(s)	147, 515, 563, 960, 1216, 1359, 2397, 2625
BstMBI	GATC	9 cut(s)	909, 1015, 1121, 1192, 1606, 1684, 1783, 2218, 2638
BstMWI	GCNNNNNNNGC	8 cut(s)	108, 347, 505, 675, 1084, 1100, 1666, 1919
BstNI	CCWGG	2 cut(s)	248, 2238
BstNSI	RCATGY	2 cut(s)	1285, 1586
BstPI	GGTNACC	1 cut(s)	1294
BstSCI	CCNGG	3 cut(s)	246, 2236, 2481
BstSFI	CTRYAG	3 cut(s)	1079, 2004, 2418
BstSLI	GKGCMC	1 cut(s)	1759
BstUI	CGCG	1 cut(s)	997
BstV2I	GAAGAC	1 cut(s)	80
BstX2I	RGATCY	4 cut(s)	1192, 1606, 1684, 2218
BstYI	RGATCY	4 cut(s)	1192, 1606, 1684, 2218
Bsu15I	ATCGAT	1 cut(s)	126
Bsu36I	CCTNAGG	1 cut(s)	1110
BsuI	GTATCC	1 cut(s)	524
BsuRI	GGCC	5 cut(s)	380, 458, 1115, 1402, 2290
BsuTUI	ATCGAT	1 cut(s)	126
BtgI	CCRYGG	1 cut(s)	937
BtgZI	GCGATG	1 cut(s)	412
BtrI	CACGTC	1 cut(s)	2234
BtsCI	GGATG	4 cut(s)	835, 1705, 2105, 2287
BtsI	GCAGTG	3 cut(s)	153, 258, 1982
BtsIMutI	CAGTG	6 cut(s)	153, 258, 577, 1275, 1982, 2351
BveI	ACCTGC	1 cut(s)	1029
CaiI	CAGNNNCTG	1 cut(s)	1444
CciI	TCATGA	1 cut(s)	1509
CfoI	GCGC	5 cut(s)	152, 671, 700, 997, 1105
Cfr10I	RCCGGY	5 cut(s)	771, 818, 1298, 1339, 2387
Cfr13I	GGNCC	2 cut(s)	769, 795
ClaI	ATCGAT	1 cut(s)	126
CseI	GACGC	1 cut(s)	545
Csp6I	GTAC	3 cut(s)	790, 1057, 1458
CspAI	ACCGGT	3 cut(s)	771, 1339, 2387
CviQI	GTAC	3 cut(s)	790, 1057, 1458
DinI	GGCGCC	1 cut(s)	151
DpnI	GATC	9 cut(s)	911, 1017, 1123, 1194, 1608, 1686, 1785, 2220, 2640
DpnII	GATC	9 cut(s)	909, 1015, 1121, 1192, 1606, 1684, 1783, 2218, 2638
Eam1104I	CTCTTC	3 cut(s)	559, 887, 1148
EarI	CTCTTC	3 cut(s)	559, 887, 1148
EciI	GGCGGA	2 cut(s)	173, 536
Ecl136II	GAGCTC	1 cut(s)	783
Eco130I	CCWWGG	4 cut(s)	937, 1177, 2108, 2548
Eco147I	AGGCCT	1 cut(s)	1402
Eco24I	GRGCYC	1 cut(s)	785
Eco31I	GGTCTC	1 cut(s)	1216
Eco32I	GATATC	1 cut(s)	868
Eco47I	GGWCC	2 cut(s)	769, 795
Eco53kI	GAGCTC	1 cut(s)	783
Eco57I	CTGAAG	1 cut(s)	2516
Eco81I	CCTNAGG	1 cut(s)	1110
Eco88I	CYCGRG	1 cut(s)	518
Eco91I	GGTNACC	1 cut(s)	1294
EcoICRI	GAGCTC	1 cut(s)	783
EcoO65I	GGTNACC	1 cut(s)	1294
EcoRII	CCWGG	2 cut(s)	246, 2236
EcoRV	GATATC	1 cut(s)	868
EcoT14I	CCWWGG	4 cut(s)	937, 1177, 2108, 2548
EcoT22I	ATGCAT	1 cut(s)	1323
EcoT38I	GRGCYC	1 cut(s)	785
EgeI	GGCGCC	1 cut(s)	151
EheI	GGCGCC	1 cut(s)	151
ErhI	CCWWGG	4 cut(s)	937, 1177, 2108, 2548
Esp3I	CGTCTC	4 cut(s)	147, 563, 1359, 2625
FalI	AAGNNNNNCTT	2 cut(s)	2042, 2074
FaqI	GGGAC	7 cut(s)	107, 153, 258, 782, 1586, 2014, 2145
FauI	CCCGC	2 cut(s)	35, 1668
FbaI	TGATCA	1 cut(s)	1015
FblI	GTMKAC	1 cut(s)	1167
FokI	GGATG	4 cut(s)	822, 1712, 2112, 2294
FriOI	GRGCYC	1 cut(s)	785
FspBI	CTAG	4 cut(s)	1125, 1593, 1769, 2109
FspI	TGCGCA	2 cut(s)	670, 699
GlaI	GCGC	5 cut(s)	151, 670, 699, 996, 1104
GsaI	CCCAGC	1 cut(s)	1441
HaeII	RGCGCY	1 cut(s)	153
HaeIII	GGCC	5 cut(s)	380, 458, 1115, 1402, 2290
HapII	CCGG	6 cut(s)	772, 819, 1299, 1340, 2388, 2483
HgaI	GACGC	1 cut(s)	545
HhaI	GCGC	5 cut(s)	152, 671, 700, 997, 1105
Hin1I	GRCGYC	2 cut(s)	150, 556
Hin6I	GCGC	5 cut(s)	150, 669, 698, 995, 1103
HinP1I	GCGC	5 cut(s)	150, 669, 698, 995, 1103
HincII	GTYRAC	1 cut(s)	2370
HindII	GTYRAC	1 cut(s)	2370
HindIII	AAGCTT	4 cut(s)	635, 900, 2338, 2507
HinfI	GANTC	9 cut(s)	288, 567, 1431, 1546, 1799, 1847, 2104, 2525, 2660
HpaII	CCGG	6 cut(s)	772, 819, 1299, 1340, 2388, 2483
HphI	GGTGA	6 cut(s)	1010, 1388, 1655, 1985, 2402, 2669
Hpy166II	GTNNAC	2 cut(s)	1168, 2370
Hpy188III	TCNNGA	9 cut(s)	518, 520, 585, 1097, 1157, 1188, 1349, 1510, 1769
Hpy8I	GTNNAC	2 cut(s)	1168, 2370
HpyAV	CCTTC	8 cut(s)	4, 492, 711, 1067, 1216, 1553, 1799, 2540
HpyCH4III	ACNGT	8 cut(s)	167, 661, 1417, 1457, 1973, 2154, 2200, 2355
HpyCH4IV	ACGT	3 cut(s)	1368, 2037, 2233
HpyF10VI	GCNNNNNNNGC	8 cut(s)	108, 347, 505, 675, 1084, 1100, 1666, 1919
HpySE526I	ACGT	3 cut(s)	1368, 2037, 2233
Hsp92I	GRCGYC	2 cut(s)	150, 556
HspAI	GCGC	5 cut(s)	150, 669, 698, 995, 1103
KasI	GGCGCC	1 cut(s)	149
Ksp22I	TGATCA	1 cut(s)	1015
Kzo9I	GATC	9 cut(s)	909, 1015, 1121, 1192, 1606, 1684, 1783, 2218, 2638
LguI	GCTCTTC	1 cut(s)	887
LmnI	GCTCC	7 cut(s)	163, 1110, 1331, 1877, 1942, 2069, 2436
LweI	GCATC	6 cut(s)	1023, 1090, 1137, 1669, 1742, 2134
MaeI	CTAG	4 cut(s)	1125, 1593, 1769, 2109
MaeII	ACGT	3 cut(s)	1368, 2037, 2233
MaeIII	GTNAC	8 cut(s)	646, 707, 1294, 1394, 1411, 1978, 2410, 2467
MalI	GATC	9 cut(s)	911, 1017, 1123, 1194, 1608, 1686, 1785, 2220, 2640
MboI	GATC	9 cut(s)	909, 1015, 1121, 1192, 1606, 1684, 1783, 2218, 2638
MfeI	CAATTG	2 cut(s)	1205, 1664
MflI	RGATCY	4 cut(s)	1192, 1606, 1684, 2218
MhlI	GDGCHC	3 cut(s)	785, 1759, 2441
Mly113I	GGCGCC	1 cut(s)	150
MlyI	GAGTC	6 cut(s)	576, 1425, 1856, 2098, 2519, 2669
MmeI	TCCRAC	1 cut(s)	1471
Mph1103I	ATGCAT	1 cut(s)	1323
MroXI	GAANNNNTTC	4 cut(s)	747, 1066, 1233, 2154
MseI	TTAA	6 cut(s)	117, 1469, 1524, 1857, 1950, 2588
MslI	CAYNNNNRTG	2 cut(s)	1280, 1326
MspA1I	CMGCKG	1 cut(s)	1552
MspCI	CTTAAG	1 cut(s)	1523
MspI	CCGG	6 cut(s)	772, 819, 1299, 1340, 2388, 2483
MspR9I	CCNGG	3 cut(s)	248, 2238, 2483
MunI	CAATTG	2 cut(s)	1205, 1664
MvaI	CCWGG	2 cut(s)	248, 2238
MvnI	CGCG	1 cut(s)	997
MwoI	GCNNNNNNNGC	8 cut(s)	108, 347, 505, 675, 1084, 1100, 1666, 1919
NarI	GGCGCC	1 cut(s)	150
NciI	CCSGG	1 cut(s)	2483
NcoI	CCATGG	1 cut(s)	937
NdeII	GATC	9 cut(s)	909, 1015, 1121, 1192, 1606, 1684, 1783, 2218, 2638
NheI	GCTAGC	1 cut(s)	1592
NlaIV	GGNNCC	5 cut(s)	151, 770, 1004, 2220, 2554
NmuCI	GTSAC	3 cut(s)	1411, 2410, 2467
NsbI	TGCGCA	2 cut(s)	670, 699
NsiI	ATGCAT	1 cut(s)	1323
NspI	RCATGY	2 cut(s)	1285, 1586
PaeR7I	CTCGAG	1 cut(s)	518
PagI	TCATGA	1 cut(s)	1509
PaqCI	CACCTGC	1 cut(s)	1029
PceI	AGGCCT	1 cut(s)	1402
PciI	ACATGT	1 cut(s)	1281
PciSI	GCTCTTC	1 cut(s)	887
PdmI	GAANNNNTTC	4 cut(s)	747, 1066, 1233, 2154
PfeI	GAWTC	3 cut(s)	288, 1546, 1799
PflFI	GACNNNGTC	1 cut(s)	2408
PflMI	CCANNNNNTGG	1 cut(s)	450
PinAI	ACCGGT	3 cut(s)	771, 1339, 2387
PleI	GAGTC	6 cut(s)	575, 1425, 1855, 2098, 2519, 2668
PluTI	GGCGCC	1 cut(s)	153
PpsI	GAGTC	6 cut(s)	575, 1425, 1855, 2098, 2519, 2668
PscI	ACATGT	1 cut(s)	1281
Psp124BI	GAGCTC	1 cut(s)	785
Psp6I	CCWGG	2 cut(s)	246, 2236
PspEI	GGTNACC	1 cut(s)	1294
PspFI	CCCAGC	1 cut(s)	1437
PspGI	CCWGG	2 cut(s)	246, 2236
PspN4I	GGNNCC	5 cut(s)	151, 770, 1004, 2220, 2554
PspPI	GGNCC	2 cut(s)	769, 795
PstI	CTGCAG	1 cut(s)	1083
PstNI	CAGNNNCTG	1 cut(s)	1444
PsuI	RGATCY	4 cut(s)	1192, 1606, 1684, 2218
PsyI	GACNNNGTC	1 cut(s)	2408
RsaI	GTAC	3 cut(s)	791, 1058, 1459
RsaNI	GTAC	3 cut(s)	790, 1057, 1458
RseI	CAYNNNNRTG	2 cut(s)	1280, 1326
SacI	GAGCTC	1 cut(s)	785
SapI	GCTCTTC	1 cut(s)	887
SaqAI	TTAA	6 cut(s)	117, 1469, 1524, 1857, 1950, 2588
Sau3AI	GATC	9 cut(s)	909, 1015, 1121, 1192, 1606, 1684, 1783, 2218, 2638
Sau96I	GGNCC	2 cut(s)	769, 795
SchI	GAGTC	6 cut(s)	576, 1425, 1856, 2098, 2519, 2669
ScrFI	CCNGG	3 cut(s)	248, 2238, 2483
SduI	GDGCHC	3 cut(s)	785, 1759, 2441
SfaNI	GCATC	6 cut(s)	1023, 1090, 1137, 1669, 1742, 2134
SfcI	CTRYAG	3 cut(s)	1079, 2004, 2418
SfoI	GGCGCC	1 cut(s)	151
Sfr274I	CTCGAG	1 cut(s)	518
SinI	GGWCC	2 cut(s)	769, 795
SlaI	CTCGAG	1 cut(s)	518
SmiMI	CAYNNNNRTG	2 cut(s)	1280, 1326
SmlI	CTYRAG	2 cut(s)	518, 1523
SmoI	CTYRAG	2 cut(s)	518, 1523
SseBI	AGGCCT	1 cut(s)	1402
SsiI	CCGC	7 cut(s)	42, 184, 547, 1550, 1596, 1675, 2373
SspDI	GGCGCC	1 cut(s)	149
SspI	AATATT	2 cut(s)	541, 1232
SspMI	CTAG	4 cut(s)	1125, 1593, 1769, 2109
SstI	GAGCTC	1 cut(s)	785
StuI	AGGCCT	1 cut(s)	1402
StyD4I	CCNGG	3 cut(s)	246, 2236, 2481
StyI	CCWWGG	4 cut(s)	937, 1177, 2108, 2548
TaaI	ACNGT	8 cut(s)	167, 661, 1417, 1457, 1973, 2154, 2200, 2355
TaiI	ACGT	3 cut(s)	1371, 2040, 2236
TatI	WGTACW	2 cut(s)	789, 1056
TfiI	GAWTC	3 cut(s)	288, 1546, 1799
Tru1I	TTAA	6 cut(s)	117, 1469, 1524, 1857, 1950, 2588
Tru9I	TTAA	6 cut(s)	117, 1469, 1524, 1857, 1950, 2588
TscAI	CASTG	6 cut(s)	160, 265, 577, 1282, 1989, 2358
TseFI	GTSAC	3 cut(s)	1411, 2410, 2467
Tsp45I	GTSAC	3 cut(s)	1411, 2410, 2467
TspGWI	ACGGA	2 cut(s)	92, 2112
TspRI	CASTG	6 cut(s)	160, 265, 577, 1282, 1989, 2358
Tth111I	GACNNNGTC	1 cut(s)	2408
Van91I	CCANNNNNTGG	1 cut(s)	450
Vha464I	CTTAAG	1 cut(s)	1523
VpaK11BI	GGWCC	2 cut(s)	769, 795
XapI	RAATTY	6 cut(s)	281, 844, 1563, 1888, 1906, 2561
XbaI	TCTAGA	1 cut(s)	1768
XceI	RCATGY	2 cut(s)	1285, 1586
XcmI	CCANNNNNNNNNTGG	2 cut(s)	451, 720
XhoI	CTCGAG	1 cut(s)	518
XmaJI	CCTAGG	1 cut(s)	2108
XmiI	GTMKAC	1 cut(s)	1167
XmnI	GAANNNNTTC	4 cut(s)	747, 1066, 1233, 2154
XspI	CTAG	4 cut(s)	1125, 1593, 1769, 2109
Zsp2I	ATGCAT	1 cut(s)	1323

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.