Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0009795

Physical Location & Seq

Genomic Coordinates Forward (+)

59021645 .. 59026050

Gene Structure 4406 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G034430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2619 bp

ATGTTGGGAGGTTCAATGGCAATGTGTGGCCAAGAGCTGGAGCAAATGTCCAGTGTCGATCAGGGCAGTCAGTATAGCCTCTCCGATGGCATTTTGCCTTCTCTTGGTGCTGCCTCTCGTAACCACCGCAGACCGAAACTTCGTCGTTTCATCATCTCCCCCTTCAATCCCCATTACAAGTTTTGGCAGTCGTTTCTCATATTGTTGGTGTTCTACACGGCATGGGTGTGTCCCTTTGAATTTGGGTTCCTGGACAGTCCAAAAGGGCCTCTGGCTATTACTGATAATGTTGTCAATGCCTTTTTTGGCATTGATATTATCTTGACGTTCTTTGTGGCTTTCCTTGATAAAACTACTTATTTGCTCATTGATGCCCCAAAGCTAATTGCTCTGAGATATGCCAAGACCTGGTTGGCCTTTGACATCATCTCCATTATCCCTTCAGAATTTGCTCAAGCGGTTTTGCCTCATCCTCTTGCGAATTATGGATACTTCAATATTCTTCGGCTATGGCGTCTCCGAAGAGTCAGTGGAATGTTTGCCAGATTGGAAAAAGATCGGAATTATAGCTACTTCTGGGTTCGATGCTGCAAGCTTATCTTTGTTACTCTGTTTACAGTTCATTGTGGGGGCTGCATCTTCTACTTCCTTGCTGCGAATTATCGTGATCCTAAAAGGACATGGCTTGGGCTTATCTCAGATAACTTTAACGATATAAGTTTGTGGAACCGGTATGTGACATCAATGTACTGGTCCATAATCACGCTCACGACCACCGGCTATGGTGATTTGCATCCTGTAAATTCTCAAGAAATGATTTTCGATATCTTCTATATGCTCTTTAATGTTGGGCTGCAAGCTTACCTGATTGGAAACATGACCAACTTAATCGTCCATGGGACTGCTCGAACTAGACAATTTAGAGATACCATTCACGCTGCGTCGAGTTTTGCTTTAAGGAACCAAATACCTGATCGTCTGCACGAACAGATGCTTGCGCATTTGTGTTTGAAGTACAGAACTAATTCAGAAGGACTGCAGCAGCAAGAGACTCTTGATGCACTCCCTCACGCCATCAGATCAAGTATCTCACACTATCTCTTCAATTCACTTGTTGACAGTGTTTACCTTTTTCAGGGGGTATCGAGAGACATGCTTTTCCAACTGGTCGCAGAGATGAAGGCCGAGTATTTTCCTCCGAAAGAAGATGTCATCTTGCAGAATGAAGCGCCCACTGACATGTATATAGTGGTTACTGGTTCTCTGGAACTTATATTCCAAAAGAATGGAGTAGAACTGGTATTTAGTGAAGCAAAGACAGGAGATGTTTTTGGTGCCATAGGTGTGCTGTGTTACAGGCCACAACTGTTCACAGTTCGGACGAAGGGACTCTGTCAGCTGCTGCGTTTGAATAGGACAGCTTTCTTGAATATTGTTCAGGCAAATGTTGGAGATGGGACAATTATCATGAGCAATTTTCTTCAGCATTTGAGGGAAATGAAGGATCCCATAATGGAAGGAATTCTAACAGATGTAGAACATATGTTGGCTAGGGGAAAGATGGACATGCCTCTTAGTTTAGGGTTTGCTGCAAAGAGGAGTGATGATTTGTTGCTACATCAATTGCTAAGACGGGGTGCAGATCCGAATGAGACAGATCATACAGGCCGGACAGCTATGCACATTGCAGCATCAAATGGAAGTCAGCATTGTGCGACTTTACTTCTAGAGTATGGAGGTGATCCTAACATCAAAGATTGTGAAGAAAATGTTCCTCTATGGGAAGCAATAATGGGCAAACATGAATCTTTGATTAAACTTTTCTTAGAAAATGGTGCTGAGATTAGTTCTGGCAATGTGGGCCATTATGCTTGTGCTGCTGTTGAGCAAAACAACTTAGAGCTGCTCAAAGACATTGTCCGATATGGTGGTGATGTTACATTGCCCAAGACCGATGGGACTACAGCACTCCATAAAGCAGTGAGTGAAGGAAATGCTGAGATAGTTAAGTTCCTCTTAGAACAAGGAGCTGATGCTGACAATAGGGATAGCTATGGATGGAGTCCGAGGGCTTTGGCAGAGCATCAGGGACATGAAGAAATAATGGAGCTGTTTCAGCACAAATCTGGAGTTAAGAAACCAACTACTGTTCCTCTGCCAAACGATCCTCAGCTACCACGCCTTGTGAAGTTCCAGAGTGAGCCCATTATGCCACCCTATGTCCGGGATGGCAGGCCAAACTCAGAAACACGGTGGTTGGATGATACTCGGATAAGAAGGTTCAACAACTTTCCCAATTCGCTTTTTGGGATCATGTCAGCTGCCAATACCGGTGAGAGAGACAAAGACCTCATCAAACCTGCAGCCAATTTTGTCCGTGGTATCCGTAGTAAGAGCAAATATCCAGCCAGAGTTACTCTTAGTTGCCCAGAAAAAAGTGGAACTGCTGCAAAGCTTGTGGTTGTGCCAGAGTCCCTTCAACAATTACTTGACATCGGTGCCAAAAAATTTCAGTTTTGTCCCACTAAAGTTTTAACCACAGAAGGAGCTGAAGTTGAAGATATAGAGCTTGTTAGGGATGGTGATCATCTTGTTCTTGTTGGTGATGGTGAGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

872

Amino Acids

98.31

Weight (kDa)

6.33

Isoelectric Point (pI)

42.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	59 - 304	1e-42	Ion transport protein
Ion_trans_2	PF07885	206 - 297	8.8e-12	Ion channel
cNMP_binding	PF00027	397 - 482	6.9e-14	Cyclic nucleotide-binding domain
Ank_2	PF12796	528 - 617	8.5e-15	Ankyrin repeats (3 copies)
Ank_4	PF13637	530 - 576	2.1e-06	Ankyrin repeats (many copies)
Ank	PF00023	556 - 586	1.7e-06	Ankyrin repeat
Ank_4	PF13637	625 - 673	3.9e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	626 - 708	7.9e-14	Ankyrin repeats (3 copies)
Ank_5	PF13857	645 - 693	5.6e-09	Ankyrin repeats (many copies)
Ank	PF00023	652 - 683	1.8e-07	Ankyrin repeat
KHA	PF11834	804 - 866	3.3e-21	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009795)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25600 AT4G32500
fragaria_vesca	FvH4_2g29700 FvH4_2g29700 FvH4_2g29700
malus_domestica	MD08G1142800.v1.1 MD15G1119300.v1.1
prunus_persica	Prupe.1G472600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0298701
rosa_laevigata	RLG00000011492
rosa_multiflora	Rmu_co8298943.1_g000001 Rmu_sc0008099.1_g000002 Rmu_sc0008142.1_g000002 Rmu_sc0032030.1_g000001
rosa_roxburghii	Rroxscaffold_7G00169400
rosa_rugosa	Rorug06G0282700
rosa_samantha	Rh6AG393800 Rh6BG401600 Rh6CG407500 Rh6DG394100
rosa_wichuraiana	Rw6G034430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	999
Acc36I	ACCTGC	1 cut(s)	2368
AccB1I	GGYRCC	2 cut(s)	1334, 2498
AccB7I	CCANNNNNTGG	2 cut(s)	37, 408
AciI	CCGC	2 cut(s)	127, 458
AclWI	GGATC	7 cut(s)	662, 1499, 1512, 1637, 1736, 2159, 2318
AcoI	YGGCCR	1 cut(s)	28
AcsI	RAATTY	5 cut(s)	239, 446, 802, 1521, 2507
AcuI	CTGAAG	3 cut(s)	426, 1466, 2571
AcyI	GRCGYC	1 cut(s)	514
AfaI	GTAC	2 cut(s)	749, 1016
AfiI	CCNNNNNNNGG	5 cut(s)	37, 104, 408, 1135, 2223
AflIII	ACRYGT	1 cut(s)	1239
AgeI	ACCGGT	2 cut(s)	729, 2330
AjnI	CCWGG	2 cut(s)	249, 407
AloI	GAACNNNNNNTCC	2 cut(s)	1260, 1292
Alw26I	GTCTC	5 cut(s)	521, 1043, 1143, 1646, 2334
AlwI	GGATC	7 cut(s)	662, 1499, 1512, 1637, 1736, 2159, 2318
AlwNI	CAGNNNCTG	1 cut(s)	1402
AoxI	GGCC	8 cut(s)	28, 266, 414, 1182, 1358, 1666, 1861, 2234
ApoI	RAATTY	5 cut(s)	239, 446, 802, 1521, 2507
ArsI	GACNNNNNNTTYG	4 cut(s)	123, 155, 1902, 1934
AsiGI	ACCGGT	2 cut(s)	729, 2330
Asp700I	GAANNNNTTC	3 cut(s)	1024, 1521, 1770
AspLEI	GCGC	2 cut(s)	1000, 1231
AspS9I	GGNCC	3 cut(s)	266, 753, 1861
AsuC2I	CCSGG	1 cut(s)	2225
AsuHPI	GGTGA	6 cut(s)	797, 1751, 1943, 2345, 2594, 2615
AvaII	GGWCC	1 cut(s)	753
BalI	TGGCCA	1 cut(s)	30
BamHI	GGATCC	1 cut(s)	1504
BanI	GGYRCC	2 cut(s)	1334, 2498
BanII	GRGCYC	1 cut(s)	2205
BbvCI	CCTCAGC	1 cut(s)	2169
BccI	CCATC	9 cut(s)	80, 1082, 1448, 1555, 1949, 2052, 2222, 2573, 2600
BceAI	ACGGC	1 cut(s)	234
BcgI	CGANNNNNNTGC	2 cut(s)	1135, 1169
BciT130I	CCWGG	2 cut(s)	251, 409
BciVI	GTATCC	2 cut(s)	482, 2393
BclI	TGATCA	1 cut(s)	2584
BcnI	CCSGG	1 cut(s)	2225
BcoDI	GTCTC	5 cut(s)	521, 1043, 1143, 1646, 2334
BfaI	CTAG	3 cut(s)	912, 1551, 1727
BfmI	CTRYAG	3 cut(s)	1037, 1962, 2361
BfoI	RGCGCY	1 cut(s)	1232
BfuAI	ACCTGC	1 cut(s)	2368
BfuI	GTATCC	2 cut(s)	482, 2393
Bme1390I	CCNGG	3 cut(s)	251, 409, 2225
Bme18I	GGWCC	1 cut(s)	753
BmgT120I	GGNCC	3 cut(s)	266, 753, 1861
BmiI	GGNNCC	6 cut(s)	248, 728, 962, 1336, 1506, 2500
BmrFI	CCNGG	3 cut(s)	251, 409, 2225
BmsI	GCATC	9 cut(s)	361, 575, 645, 802, 981, 1048, 1700, 2023, 2092
BpmI	CTGGAG	2 cut(s)	59, 2148
Bpu10I	CCTNAGC	1 cut(s)	2169
BpuEI	CTTGAG	2 cut(s)	438, 792
BpuMI	CCSGG	1 cut(s)	2225
BsaBI	GATNNNNATC	2 cut(s)	792, 2583
BsaHI	GRCGYC	1 cut(s)	514
BsaJI	CCNNGG	3 cut(s)	895, 2066, 2377
BsaWI	WCCGGW	2 cut(s)	729, 2330
BsaXI	ACNNNNNCTCC	6 cut(s)	32, 62, 413, 443, 2538, 2568
Bsc4I	CCNNNNNNNGG	5 cut(s)	37, 104, 408, 1135, 2223
Bse118I	RCCGGY	3 cut(s)	729, 776, 2330
Bse1I	ACTGG	5 cut(s)	51, 755, 1170, 1261, 1302
Bse3DI	GCAATG	4 cut(s)	27, 1683, 1861, 1940
Bse8I	GATNNNNATC	2 cut(s)	792, 2583
BseBI	CCWGG	2 cut(s)	251, 409
BseDI	CCNNGG	3 cut(s)	895, 2066, 2377
BseGI	GGATG	6 cut(s)	469, 793, 2063, 2233, 2266, 2584
BseJI	GATNNNNATC	2 cut(s)	792, 2583
BseLI	CCNNNNNNNGG	5 cut(s)	37, 104, 408, 1135, 2223
BseMI	GCAATG	4 cut(s)	27, 1683, 1861, 1940
BseMII	CTCAG	6 cut(s)	383, 711, 1830, 1989, 2183, 2256
BseNI	ACTGG	5 cut(s)	51, 755, 1170, 1261, 1302
BseRI	GAGGAG	1 cut(s)	1612
BsgI	GTGCAG	2 cut(s)	965, 1659
BshFI	GGCC	8 cut(s)	30, 268, 416, 1184, 1360, 1668, 1863, 2236
BshNI	GGYRCC	2 cut(s)	1334, 2498
BshTI	ACCGGT	2 cut(s)	729, 2330
BsiSI	CCGG	5 cut(s)	730, 777, 1669, 2224, 2331
BslFI	GGGAC	8 cut(s)	216, 913, 1401, 1471, 1972, 2103, 2458, 2505
BslI	CCNNNNNNNGG	5 cut(s)	37, 104, 408, 1135, 2223
BsmAI	GTCTC	5 cut(s)	521, 1043, 1143, 1646, 2334
BsmBI	CGTCTC	1 cut(s)	521
BsmFI	GGGAC	8 cut(s)	216, 913, 1401, 1471, 1972, 2103, 2458, 2505
BsnI	GGCC	8 cut(s)	30, 268, 416, 1184, 1360, 1668, 1863, 2236
Bsp1286I	GDGCHC	1 cut(s)	2205
Bsp19I	CCATGG	1 cut(s)	895
BspACI	CCGC	2 cut(s)	127, 458
BspANI	GGCC	8 cut(s)	30, 268, 416, 1184, 1360, 1668, 1863, 2236
BspCNI	CTCAG	6 cut(s)	384, 710, 1831, 1990, 2182, 2255
BspHI	TCATGA	1 cut(s)	1467
BspLI	GGNNCC	6 cut(s)	248, 728, 962, 1336, 1506, 2500
BspMAI	CTGCAG	2 cut(s)	1041, 2365
BspMI	ACCTGC	1 cut(s)	2368
BspPI	GGATC	7 cut(s)	662, 1499, 1512, 1637, 1736, 2159, 2318
BspT107I	GGYRCC	2 cut(s)	1334, 2498
BsrDI	GCAATG	4 cut(s)	27, 1683, 1861, 1940
BsrFI	RCCGGY	3 cut(s)	729, 776, 2330
BsrI	ACTGG	5 cut(s)	51, 755, 1170, 1261, 1302
BssAI	RCCGGY	3 cut(s)	729, 776, 2330
BssECI	CCNNGG	3 cut(s)	895, 2066, 2377
BssNI	GRCGYC	1 cut(s)	514
BssT1I	CCWWGG	1 cut(s)	895
Bst2UI	CCWGG	2 cut(s)	251, 409
Bst4CI	ACNGT	7 cut(s)	257, 619, 1121, 1368, 1375, 2149, 2253
Bst6I	CTCTTC	2 cut(s)	517, 1106
BstACI	GRCGYC	1 cut(s)	514
BstC8I	GCNNGC	4 cut(s)	593, 858, 996, 2234
BstDSI	CCRYGG	2 cut(s)	895, 2377
BstENI	CCTNNNNNAGG	1 cut(s)	1133
BstF5I	GGATG	6 cut(s)	469, 793, 2063, 2233, 2266, 2584
BstH2I	RGCGCY	1 cut(s)	1232
BstHHI	GCGC	2 cut(s)	1000, 1231
BstMAI	GTCTC	5 cut(s)	521, 1043, 1143, 1646, 2334
BstMWI	GCNNNNNNNGC	4 cut(s)	1860, 1877, 2116, 2209
BstNI	CCWGG	2 cut(s)	251, 409
BstNSI	RCATGY	3 cut(s)	1156, 1243, 1570
BstSCI	CCNGG	3 cut(s)	249, 407, 2223
BstSFI	CTRYAG	3 cut(s)	1037, 1962, 2361
BstX2I	RGATCY	2 cut(s)	1504, 1642
BstXI	CCANNNNNNTGG	1 cut(s)	1286
BstYI	RGATCY	2 cut(s)	1504, 1642
BsuI	GTATCC	2 cut(s)	482, 2393
BsuRI	GGCC	8 cut(s)	30, 268, 416, 1184, 1360, 1668, 1863, 2236
BtgI	CCRYGG	2 cut(s)	895, 2377
BtsCI	GGATG	6 cut(s)	469, 793, 2063, 2233, 2266, 2584
BtsI	GCAGTG	1 cut(s)	1986
BtsIMutI	CAGTG	5 cut(s)	58, 535, 1126, 1233, 1986
BveI	ACCTGC	1 cut(s)	2368
Cac8I	GCNNGC	4 cut(s)	593, 858, 996, 2234
CaiI	CAGNNNCTG	1 cut(s)	1402
CciI	TCATGA	1 cut(s)	1467
CfoI	GCGC	2 cut(s)	1000, 1231
Cfr10I	RCCGGY	3 cut(s)	729, 776, 2330
Cfr13I	GGNCC	3 cut(s)	266, 753, 1861
CseI	GACGC	2 cut(s)	503, 930
CsiI	ACCWGGT	1 cut(s)	407
Csp6I	GTAC	2 cut(s)	748, 1015
CspAI	ACCGGT	2 cut(s)	729, 2330
CspCI	CAANNNNNGTGG	2 cut(s)	2439, 2474
CviQI	GTAC	2 cut(s)	748, 1015
EaeI	YGGCCR	1 cut(s)	28
Eam1104I	CTCTTC	2 cut(s)	517, 1106
EarI	CTCTTC	2 cut(s)	517, 1106
Eco130I	CCWWGG	1 cut(s)	895
Eco24I	GRGCYC	1 cut(s)	2205
Eco32I	GATATC	1 cut(s)	826
Eco47I	GGWCC	1 cut(s)	753
Eco57I	CTGAAG	3 cut(s)	426, 1466, 2571
EcoNI	CCTNNNNNAGG	1 cut(s)	1133
EcoO109I	RGGNCCY	1 cut(s)	266
EcoRI	GAATTC	1 cut(s)	1521
EcoRII	CCWGG	2 cut(s)	249, 407
EcoRV	GATATC	1 cut(s)	826
EcoT14I	CCWWGG	1 cut(s)	895
EcoT38I	GRGCYC	1 cut(s)	2205
ErhI	CCWWGG	1 cut(s)	895
Esp3I	CGTCTC	1 cut(s)	521
FalI	AAGNNNNNCTT	6 cut(s)	584, 616, 1038, 1070, 2000, 2032
FaqI	GGGAC	8 cut(s)	216, 913, 1401, 1471, 1972, 2103, 2458, 2505
FauNDI	CATATG	1 cut(s)	1542
FbaI	TGATCA	1 cut(s)	2584
FokI	GGATG	6 cut(s)	456, 780, 2070, 2240, 2273, 2591
FriOI	GRGCYC	1 cut(s)	2205
FspBI	CTAG	3 cut(s)	912, 1551, 1727
FspI	TGCGCA	1 cut(s)	999
GlaI	GCGC	2 cut(s)	999, 1230
GsuI	CTGGAG	2 cut(s)	59, 2148
HaeII	RGCGCY	1 cut(s)	1232
HaeIII	GGCC	8 cut(s)	30, 268, 416, 1184, 1360, 1668, 1863, 2236
HapII	CCGG	5 cut(s)	730, 777, 1669, 2224, 2331
HgaI	GACGC	2 cut(s)	503, 930
HhaI	GCGC	2 cut(s)	1000, 1231
Hin1I	GRCGYC	1 cut(s)	514
Hin6I	GCGC	2 cut(s)	998, 1229
HinP1I	GCGC	2 cut(s)	998, 1229
HincII	GTYRAC	1 cut(s)	1117
HindII	GTYRAC	1 cut(s)	1117
HindIII	AAGCTT	3 cut(s)	593, 858, 2453
HinfI	GANTC	6 cut(s)	525, 1051, 1389, 1805, 2062, 2471
HpaII	CCGG	5 cut(s)	730, 777, 1669, 2224, 2331
HphI	GGTGA	6 cut(s)	797, 1751, 1943, 2345, 2594, 2615
Hpy166II	GTNNAC	4 cut(s)	615, 1117, 1126, 1371
Hpy8I	GTNNAC	4 cut(s)	615, 1117, 1126, 1371
Hpy99I	CGWCG	2 cut(s)	147, 946
HpyCH4III	ACNGT	7 cut(s)	257, 619, 1121, 1368, 1375, 2149, 2253
HpyCH4IV	ACGT	1 cut(s)	326
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1860, 1877, 2116, 2209
HpySE526I	ACGT	1 cut(s)	326
Hsp92I	GRCGYC	1 cut(s)	514
HspAI	GCGC	2 cut(s)	998, 1229
Ksp22I	TGATCA	1 cut(s)	2584
LmnI	GCTCC	4 cut(s)	40, 2027, 2107, 2546
LweI	GCATC	9 cut(s)	361, 575, 645, 802, 981, 1048, 1700, 2023, 2092
MabI	ACCWGGT	1 cut(s)	407
MaeI	CTAG	3 cut(s)	912, 1551, 1727
MaeII	ACGT	1 cut(s)	326
MaeIII	GTNAC	7 cut(s)	119, 604, 736, 1252, 1352, 1936, 2413
MfeI	CAATTG	1 cut(s)	1622
MflI	RGATCY	2 cut(s)	1504, 1642
MhlI	GDGCHC	1 cut(s)	2205
MlsI	TGGCCA	1 cut(s)	30
MluNI	TGGCCA	1 cut(s)	30
MlyI	GAGTC	5 cut(s)	534, 1045, 1383, 2071, 2480
MmeI	TCCRAC	3 cut(s)	1186, 1429, 2238
Mox20I	TGGCCA	1 cut(s)	30
MroXI	GAANNNNTTC	3 cut(s)	1024, 1521, 1770
MscI	TGGCCA	1 cut(s)	30
MseI	TTAA	8 cut(s)	708, 843, 887, 956, 1815, 2007, 2133, 2534
MslI	CAYNNNNRTG	3 cut(s)	226, 1238, 1343
Msp20I	TGGCCA	1 cut(s)	30
MspA1I	CMGCKG	2 cut(s)	1399, 2321
MspI	CCGG	5 cut(s)	730, 777, 1669, 2224, 2331
MspR9I	CCNGG	3 cut(s)	251, 409, 2225
MunI	CAATTG	1 cut(s)	1622
MvaI	CCWGG	2 cut(s)	251, 409
MwoI	GCNNNNNNNGC	4 cut(s)	1860, 1877, 2116, 2209
NciI	CCSGG	1 cut(s)	2225
NcoI	CCATGG	1 cut(s)	895
NdeI	CATATG	1 cut(s)	1542
NlaIV	GGNNCC	6 cut(s)	248, 728, 962, 1336, 1506, 2500
NmeAIII	GCCGAG	1 cut(s)	1210
NmuCI	GTSAC	1 cut(s)	736
NsbI	TGCGCA	1 cut(s)	999
NspI	RCATGY	3 cut(s)	1156, 1243, 1570
PagI	TCATGA	1 cut(s)	1467
PciI	ACATGT	1 cut(s)	1239
PcsI	WCGNNNNNNNCGW	1 cut(s)	511
PdmI	GAANNNNTTC	3 cut(s)	1024, 1521, 1770
PfeI	GAWTC	1 cut(s)	1805
PflFI	GACNNNGTC	2 cut(s)	1392, 1916
PflMI	CCANNNNNTGG	2 cut(s)	37, 408
PfoI	TCCNGGA	2 cut(s)	249, 2223
PinAI	ACCGGT	2 cut(s)	729, 2330
PleI	GAGTC	5 cut(s)	533, 1045, 1383, 2070, 2479
PpsI	GAGTC	5 cut(s)	533, 1045, 1383, 2070, 2479
PscI	ACATGT	1 cut(s)	1239
Psp6I	CCWGG	2 cut(s)	249, 407
PspGI	CCWGG	2 cut(s)	249, 407
PspN4I	GGNNCC	6 cut(s)	248, 728, 962, 1336, 1506, 2500
PspPI	GGNCC	3 cut(s)	266, 753, 1861
PstI	CTGCAG	2 cut(s)	1041, 2365
PstNI	CAGNNNCTG	1 cut(s)	1402
PsuI	RGATCY	2 cut(s)	1504, 1642
PsyI	GACNNNGTC	2 cut(s)	1392, 1916
PvuII	CAGCTG	2 cut(s)	1399, 2321
RsaI	GTAC	2 cut(s)	749, 1016
RsaNI	GTAC	2 cut(s)	748, 1015
RseI	CAYNNNNRTG	3 cut(s)	226, 1238, 1343
SaqAI	TTAA	8 cut(s)	708, 843, 887, 956, 1815, 2007, 2133, 2534
Sau96I	GGNCC	3 cut(s)	266, 753, 1861
SchI	GAGTC	5 cut(s)	534, 1045, 1383, 2071, 2480
ScrFI	CCNGG	3 cut(s)	251, 409, 2225
SduI	GDGCHC	1 cut(s)	2205
SexAI	ACCWGGT	1 cut(s)	407
SfaNI	GCATC	9 cut(s)	361, 575, 645, 802, 981, 1048, 1700, 2023, 2092
SfcI	CTRYAG	3 cut(s)	1037, 1962, 2361
SinI	GGWCC	1 cut(s)	753
SmiMI	CAYNNNNRTG	3 cut(s)	226, 1238, 1343
SmlI	CTYRAG	2 cut(s)	453, 807
SmoI	CTYRAG	2 cut(s)	453, 807
SsiI	CCGC	2 cut(s)	127, 458
SspI	AATATT	2 cut(s)	499, 1432
SspMI	CTAG	3 cut(s)	912, 1551, 1727
StyD4I	CCNGG	3 cut(s)	249, 407, 2223
StyI	CCWWGG	1 cut(s)	895
TaaI	ACNGT	7 cut(s)	257, 619, 1121, 1368, 1375, 2149, 2253
TaiI	ACGT	1 cut(s)	329
TaqI	TCGA	6 cut(s)	57, 583, 822, 907, 944, 1145
TaqII	GACCGA	2 cut(s)	148, 1967
TatI	WGTACW	2 cut(s)	747, 1014
TfiI	GAWTC	1 cut(s)	1805
Tru1I	TTAA	8 cut(s)	708, 843, 887, 956, 1815, 2007, 2133, 2534
Tru9I	TTAA	8 cut(s)	708, 843, 887, 956, 1815, 2007, 2133, 2534
TscAI	CASTG	5 cut(s)	58, 535, 1126, 1240, 1986
TseFI	GTSAC	1 cut(s)	736
Tsp45I	GTSAC	1 cut(s)	736
TspDTI	ATGAA	7 cut(s)	139, 611, 1193, 1239, 1514, 1818, 2109
TspGWI	ACGGA	2 cut(s)	2366, 2375
TspRI	CASTG	5 cut(s)	58, 535, 1126, 1240, 1986
Tth111I	GACNNNGTC	2 cut(s)	1392, 1916
Van91I	CCANNNNNTGG	2 cut(s)	37, 408
VpaK11BI	GGWCC	1 cut(s)	753
XagI	CCTNNNNNAGG	1 cut(s)	1133
XapI	RAATTY	5 cut(s)	239, 446, 802, 1521, 2507
XbaI	TCTAGA	1 cut(s)	1726
XceI	RCATGY	3 cut(s)	1156, 1243, 1570
XcmI	CCANNNNNNNNNTGG	1 cut(s)	409
XmnI	GAANNNNTTC	3 cut(s)	1024, 1521, 1770
XspI	CTAG	3 cut(s)	912, 1551, 1727

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.