Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009795

Physical Location & Seq

Genomic Coordinates Reverse (-)

8581968 .. 8586721

Gene Structure 4754 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1119300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2676 bp

ATGATGAGGAAGGGATCAATGTCAATGTCGATGTTAATATGCGGGCAGGGGTATGGCGGAGTAGAACAGGAGAAGGCGGAACAAATGTCGAGGGAAGACAGCAGCCATGCCAGCCAGTTTAGCCTCAGTAATGGCATTTTGCCGTCTCTTGGTGCCACTGCTCGAAACCGTCCCAAACACAAGCTCCACCGCTTTATCATCTCCCCATTTAATTATTATTACAAACTTTGGCAGTCATTTCTTATATTGCTGGTTTTCTACACTGCTTGGGTGTGTCCCTTTGAATTTGGATTCTTGGACAGAGCAAAAGGACCTCTTGCCATAGCTGATAACGTTGTCAATGCACTTTTCGCTATCGACATTGTATTGACATTCTTTATAGCCTACCTTGATAAAACTACTTATCTCCTCATTGACAACCACAAGATCATTGCTCTGAGATATGCCAAAACCTGGCTGGCCTTTGACATCATCTCCACAATCCCTTGGGAACTTGCTCAGCGTGTTCTGCCTCCTCCTCTGGAGACTTATGGATACTTCAATATCCTCCGCTTTTGGCGTCTCCGAAGATTCAGTGCAATGATTTCCAGATTGGAGAAGGACAGGAACTATAGCTACTTTTGGGTTCGATACTGCAAACTTATTTTCGTTACTCTCCTCACAGTTCATTGCGCCGGCTGCTTCTTCTATTTCCTTGCTGTGCATTATCCTAACCCAAGAAGGACATGGTTTGGGCTTATCGTAGAAAACTTTCAACAATTGAGTTTATGGGACCGGTACGTGACCTCAATGTACTGGTCCATAATCACCCTAACAACCACCGGCTATGGTGATTTTCATCCTGTAAATTCACAGGAGATGACATTCAATATCTTCTATATGCTCTTCAATGTTGGGTTGCAAGCTTACCTGATCGGAAACATGACCAACTTGATTGTCCATGGAACTGCTCGAACTAGACAATTTAGAGATAGTATTCAAGCTGCGACAAGTTTTGCACATAGGAACCAAATACCTGATCACCTGCACGAGCAGATGCTTGCTCATTTGTGTTTGAAGTACAGAACGAATTCAGAAGGGCTGCAGCAGCAAGAGGCTCTCGATGCGCTCCCTAAAGCCATTCGATGTAGCATCTCTCACTATCTCTTCTATCCACTCGTTGATAGTGTCTACTTGTTCCAGGGTGTATCAAGAGACCTCCTTTTCCAATTGGTCTCCGAGATGAAGGCTGAATATTTTCCTCCAAAAGAAGATGTAATCTTGCAGAATGAAGCACCCACTGACATGTATATCGTGGCTACTGGCTCTGTGGAACTTATTATGCATAGGAATGGAGCAACACCGGTTGTTCATGAAGCAAAGACAGGAGACGTTGTTGGTGAAATAGGTGTGCTTTGTTACAGGCCTCAAATGGTCACAGTTAGGACGAAAGGACTCTCCCAGCTTCTGCGTTTGAACCGTACAGCTTTCTTAAATCTTGTTCAAGCAAATGTTGGAGATGGAACGATAATCATGAACAATTTTCTTAAGCATTTGAAAGAAATGAAGAATCCGCTGGCAGAAGGAATTTTGAGGGACATAGAGCATGTGCTGGCTAGCGGTAAAATGGATCTGCCTCTTAGTTTATGCTTTGCTGCACAGAGAGGTGATGATTTGTTGCTGCATCGGTTGCTCAGGAGGGGTTCAGATCCAAACGAGATGGACAATAATGGACGAACAGCTATGCACATTGCAGCATCTAATGGAAATGAACACTGCGCCCTTTTACTCCTAGAGTATGAAGCAGATCCCAATATACAAGATTCGGAAGGAAATGTTCCTATATGGGAAGCAATATCAGGGAAGCATGAGTCTTTGATTAAACTTCTCTTAGACAACGGAGCGAAGATAAATTCTGGCAATGTGGGAAATTTTGCTTGTGCTGCTGTTGAGCAAAACAACTTGGAGCTGCTTAAAGACATTGTCACGTATAACGGTGATGTAACACTGCCCAAGAGCAATGGGACTGCAGCGCTTCACACGGCGGTATGTGAAGGAAATGTAGAGATGGTTAAGTTCCTCTTGGAACAAGGAGCTGAGGCTGACAAGCCGGATGGCTATGGATGGACTCCTAGGGCTTTGGCAGAGCATCAGGGACATGAAGAAATAATGGAACTGTTTCAGCACAAATGGGAGAGTAATAAACCAACCAAGAAACCAACTGTTGTTCCTATGCCAAAGGATCTGATACCATCACGCCCTGGGAAGTTCAGGAGTGAGCCTACATTACCTCCCTACGCACGGGATGGCAGGCCATCTTACTCAGATATGACATCAGAAAATACTCGTAGAATGGATAAAATCTTTCGCAATTCACTGTTTGGTTTCATGTCAGCTGCCAATACTAATACTGGTGAGAAAGATAGAGACATTGTCGCATCTACAGGCAGTTTGGCAAGGAGCACGAGTAGGAACAGCTATCCGGCCAGAGTGACACTTAGCTGCCCGAAAAAAGGCCAAACTGCTGCAAAGCTTGTGCTTCTGCCTGACTCCCTTCAACAACTACTTGACCTTGGTGCCAAGAAATTCCAGTTTACAGCCACCAAAGTTTTAACCGAAGACGGAGCTGAAGTAGAAGATGTAGAGCTTGTTAGAGACGGTGATCATCTTGTTCTTGCTGGTGATTCTGAGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ank_4 Ion_trans KHA cNMP_binding

Protein Analysis

892

Amino Acids

100.69

Weight (kDa)

6.96

Isoelectric Point (pI)

36.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	74 - 319	8.5e-40	Ion transport protein
Ion_trans_2	PF07885	220 - 312	4.4e-12	Ion channel
cNMP_binding	PF00027	412 - 497	9.6e-15	Cyclic nucleotide-binding domain
Ank_2	PF12796	542 - 632	8.8e-18	Ankyrin repeats (3 copies)
Ank_4	PF13637	542 - 591	3.4e-10	Ankyrin repeats (many copies)
Ank_5	PF13857	557 - 607	3.1e-06	Ankyrin repeats (many copies)
Ank	PF00023	570 - 601	2.6e-07	Ankyrin repeat
Ank_2	PF12796	641 - 721	8.3e-12	Ankyrin repeats (3 copies)
KHA	PF11834	823 - 885	3e-22	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Image

Tree File

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Orthologous Genes (Group: OG0009795)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25600 AT4G32500
fragaria_vesca	FvH4_2g29700 FvH4_2g29700 FvH4_2g29700
malus_domestica	MD08G1142800.v1.1 MD15G1119300.v1.1
prunus_persica	Prupe.1G472600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0298701
rosa_laevigata	RLG00000011492
rosa_multiflora	Rmu_co8298943.1_g000001 Rmu_sc0008099.1_g000002 Rmu_sc0008142.1_g000002 Rmu_sc0032030.1_g000001
rosa_roxburghii	Rroxscaffold_7G00169400
rosa_rugosa	Rorug06G0282700
rosa_samantha	Rh6AG393800 Rh6BG401600 Rh6CG407500 Rh6DG394100
rosa_wichuraiana	Rw6G034430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1032
Acc36I	ACCTGC	1 cut(s)	1032
AccB1I	GGYRCC	2 cut(s)	152, 2555
AccB7I	CCANNNNNTGG	1 cut(s)	453
AccI	GTMKAC	1 cut(s)	1170
AciI	CCGC	8 cut(s)	42, 57, 77, 190, 550, 1553, 1599, 2024
AclI	AACGTT	1 cut(s)	333
AclWI	GGATC	5 cut(s)	22, 1617, 1682, 1781, 2229
AcoI	YGGCCR	1 cut(s)	2463
AcsI	RAATTY	7 cut(s)	284, 847, 1069, 1566, 1891, 1909, 2564
AcuI	CTGAAG	1 cut(s)	2628
AcyI	GRCGYC	1 cut(s)	559
AfaI	GTAC	4 cut(s)	779, 794, 1061, 1462
AfeI	AGCGCT	1 cut(s)	2013
AfiI	CCNNNNNNNGG	4 cut(s)	149, 453, 2280, 2492
AflII	CTTAAG	1 cut(s)	1526
AflIII	ACRYGT	1 cut(s)	1284
AgeI	ACCGGT	2 cut(s)	774, 1342
AjnI	CCWGG	3 cut(s)	452, 1179, 2239
Alw21I	GWGCWC	1 cut(s)	2444
Alw26I	GTCTC	8 cut(s)	150, 518, 566, 1188, 1219, 1362, 2400, 2628
AlwI	GGATC	5 cut(s)	22, 1617, 1682, 1781, 2229
AlwNI	CAGNNNCTG	1 cut(s)	1447
Aor51HI	AGCGCT	1 cut(s)	2013
AoxI	GGCC	5 cut(s)	459, 1403, 2291, 2463, 2494
ApoI	RAATTY	7 cut(s)	284, 847, 1069, 1566, 1891, 1909, 2564
AsiGI	ACCGGT	2 cut(s)	774, 1342
Asp700I	GAANNNNTTC	4 cut(s)	750, 1069, 1236, 2157
AspA2I	CCTAGG	1 cut(s)	2111
AspLEI	GCGC	4 cut(s)	674, 1108, 1760, 2014
AspS9I	GGNCC	3 cut(s)	311, 772, 798
AsuHPI	GGTGA	9 cut(s)	799, 842, 1013, 1391, 1658, 1988, 2405, 2651, 2672
AsuNHI	GCTAGC	1 cut(s)	1595
AvaII	GGWCC	3 cut(s)	311, 772, 798
AvrII	CCTAGG	1 cut(s)	2111
BanI	GGYRCC	2 cut(s)	152, 2555
BauI	CACGAG	2 cut(s)	1028, 2443
BbsI	GAAGAC	2 cut(s)	102, 2604
Bbv12I	GWGCWC	1 cut(s)	2444
BbvCI	CCTCAGC	1 cut(s)	2076
BccI	CCATC	8 cut(s)	1493, 1693, 2041, 2087, 2097, 2239, 2279, 2302
BceAI	ACGGC	2 cut(s)	127, 2037
BciT130I	CCWGG	3 cut(s)	454, 1181, 2241
BciVI	GTATCC	1 cut(s)	527
BclI	TGATCA	2 cut(s)	1018, 2641
BcoDI	GTCTC	8 cut(s)	150, 518, 566, 1188, 1219, 1362, 2400, 2628
BfaI	CTAG	4 cut(s)	957, 1596, 1772, 2112
BfmI	CTRYAG	4 cut(s)	610, 1082, 2007, 2421
BfoI	RGCGCY	1 cut(s)	2015
BfrI	CTTAAG	1 cut(s)	1526
BfuAI	ACCTGC	1 cut(s)	1032
BfuI	GTATCC	1 cut(s)	527
BlnI	CCTAGG	1 cut(s)	2111
BlpI	GCTNAGC	1 cut(s)	498
Bme1390I	CCNGG	3 cut(s)	454, 1181, 2241
Bme18I	GGWCC	3 cut(s)	311, 772, 798
BmgT120I	GGNCC	3 cut(s)	311, 772, 798
BmiI	GGNNCC	4 cut(s)	154, 773, 1007, 2557
BmrFI	CCNGG	3 cut(s)	454, 1181, 2241
BmsI	GCATC	7 cut(s)	1026, 1093, 1140, 1672, 1745, 2137, 2426
BmtI	GCTAGC	1 cut(s)	1599
BpiI	GAAGAC	2 cut(s)	102, 2604
BpmI	CTGGAG	1 cut(s)	542
Bpu10I	CCTNAGC	2 cut(s)	1673, 2076
Bpu1102I	GCTNAGC	1 cut(s)	498
BsaAI	YACGTR	2 cut(s)	781, 1968
BsaBI	GATNNNNATC	1 cut(s)	837
BsaHI	GRCGYC	1 cut(s)	559
BsaI	GGTCTC	2 cut(s)	1188, 1219
BsaJI	CCNNGG	7 cut(s)	485, 940, 1180, 2111, 2239, 2240, 2551
BsaWI	WCCGGW	2 cut(s)	774, 1342
Bsc4I	CCNNNNNNNGG	4 cut(s)	149, 453, 2280, 2492
Bse118I	RCCGGY	4 cut(s)	674, 774, 821, 1342
Bse1I	ACTGG	5 cut(s)	115, 800, 1306, 2395, 2569
Bse3DI	GCAATG	6 cut(s)	429, 585, 667, 1728, 1906, 2005
Bse8I	GATNNNNATC	1 cut(s)	837
BseBI	CCWGG	3 cut(s)	454, 1181, 2241
BseDI	CCNNGG	7 cut(s)	485, 940, 1180, 2111, 2239, 2240, 2551
BseGI	GGATG	4 cut(s)	838, 2098, 2108, 2290
BseJI	GATNNNNATC	1 cut(s)	837
BseLI	CCNNNNNNNGG	4 cut(s)	149, 453, 2280, 2492
BseMI	GCAATG	6 cut(s)	429, 585, 667, 1728, 1906, 2005
BseMII	CTCAG	7 cut(s)	139, 428, 512, 1687, 2067, 2316, 2658
BseNI	ACTGG	5 cut(s)	115, 800, 1306, 2395, 2569
BseRI	GAGGAG	4 cut(s)	398, 504, 507, 647
BseYI	CCCAGC	1 cut(s)	1440
BsgI	GTGCAG	2 cut(s)	1010, 1620
BshFI	GGCC	5 cut(s)	461, 1405, 2293, 2465, 2496
BshNI	GGYRCC	2 cut(s)	152, 2555
BshTI	ACCGGT	2 cut(s)	774, 1342
BsiHKAI	GWGCWC	1 cut(s)	2444
BsiSI	CCGG	6 cut(s)	675, 775, 822, 1343, 2090, 2462
BslFI	GGGAC	6 cut(s)	156, 261, 785, 1589, 2017, 2148
BslI	CCNNNNNNNGG	4 cut(s)	149, 453, 2280, 2492
BsmAI	GTCTC	8 cut(s)	150, 518, 566, 1188, 1219, 1362, 2400, 2628
BsmBI	CGTCTC	4 cut(s)	150, 566, 1362, 2628
BsmFI	GGGAC	6 cut(s)	156, 261, 785, 1589, 2017, 2148
BsnI	GGCC	5 cut(s)	461, 1405, 2293, 2465, 2496
Bso31I	GGTCTC	2 cut(s)	1188, 1219
Bsp1286I	GDGCHC	1 cut(s)	2444
Bsp143I	GATC	9 cut(s)	14, 426, 912, 1018, 1609, 1687, 1786, 2221, 2641
Bsp1720I	GCTNAGC	1 cut(s)	498
Bsp19I	CCATGG	1 cut(s)	940
BspACI	CCGC	8 cut(s)	42, 57, 77, 190, 550, 1553, 1599, 2024
BspANI	GGCC	5 cut(s)	461, 1405, 2293, 2465, 2496
BspCNI	CTCAG	7 cut(s)	138, 429, 511, 1686, 2068, 2315, 2659
BspHI	TCATGA	2 cut(s)	1351, 1512
BspLI	GGNNCC	4 cut(s)	154, 773, 1007, 2557
BspMAI	CTGCAG	2 cut(s)	1086, 2011
BspMI	ACCTGC	1 cut(s)	1032
BspOI	GCTAGC	1 cut(s)	1599
BspPI	GGATC	5 cut(s)	22, 1617, 1682, 1781, 2229
BspQI	GCTCTTC	1 cut(s)	890
BspT107I	GGYRCC	2 cut(s)	152, 2555
BspTI	CTTAAG	1 cut(s)	1526
BspTNI	GGTCTC	2 cut(s)	1188, 1219
BsrDI	GCAATG	6 cut(s)	429, 585, 667, 1728, 1906, 2005
BsrFI	RCCGGY	4 cut(s)	674, 774, 821, 1342
BsrI	ACTGG	5 cut(s)	115, 800, 1306, 2395, 2569
BssAI	RCCGGY	4 cut(s)	674, 774, 821, 1342
BssECI	CCNNGG	7 cut(s)	485, 940, 1180, 2111, 2239, 2240, 2551
BssMI	GATC	9 cut(s)	14, 426, 912, 1018, 1609, 1687, 1786, 2221, 2641
BssNI	GRCGYC	1 cut(s)	559
BssSI	CACGAG	2 cut(s)	1028, 2443
BssT1I	CCWWGG	4 cut(s)	485, 940, 2111, 2551
Bst2BI	CACGAG	2 cut(s)	1028, 2443
Bst2UI	CCWGG	3 cut(s)	454, 1181, 2241
Bst4CI	ACNGT	9 cut(s)	170, 664, 1420, 1460, 1976, 2157, 2203, 2358, 2639
Bst6I	CTCTTC	2 cut(s)	890, 1151
BstACI	GRCGYC	1 cut(s)	559
BstAFI	CTTAAG	1 cut(s)	1526
BstAPI	GCANNNNNTGC	1 cut(s)	1669
BstBAI	YACGTR	2 cut(s)	781, 1968
BstDSI	CCRYGG	1 cut(s)	940
BstF5I	GGATG	4 cut(s)	838, 2098, 2108, 2290
BstH2I	RGCGCY	1 cut(s)	2015
BstHHI	GCGC	4 cut(s)	674, 1108, 1760, 2014
BstKTI	GATC	9 cut(s)	17, 429, 915, 1021, 1612, 1690, 1789, 2224, 2644
BstMAI	GTCTC	8 cut(s)	150, 518, 566, 1188, 1219, 1362, 2400, 2628
BstMBI	GATC	9 cut(s)	14, 426, 912, 1018, 1609, 1687, 1786, 2221, 2641
BstMWI	GCNNNNNNNGC	9 cut(s)	111, 120, 350, 508, 678, 1087, 1103, 1669, 1922
BstNI	CCWGG	3 cut(s)	454, 1181, 2241
BstNSI	RCATGY	2 cut(s)	1288, 1589
BstSCI	CCNGG	3 cut(s)	452, 1179, 2239
BstSFI	CTRYAG	4 cut(s)	610, 1082, 2007, 2421
BstV2I	GAAGAC	2 cut(s)	102, 2604
BstX2I	RGATCY	4 cut(s)	1609, 1687, 1786, 2221
BstYI	RGATCY	4 cut(s)	1609, 1687, 1786, 2221
BsuI	GTATCC	1 cut(s)	527
BsuRI	GGCC	5 cut(s)	461, 1405, 2293, 2465, 2496
BtgI	CCRYGG	1 cut(s)	940
BtsCI	GGATG	4 cut(s)	838, 2098, 2108, 2290
BtsI	GCAGTG	4 cut(s)	156, 261, 1753, 1985
BtsIMutI	CAGTG	7 cut(s)	156, 261, 580, 1278, 1753, 1985, 2354
BveI	ACCTGC	1 cut(s)	1032
CaiI	CAGNNNCTG	1 cut(s)	1447
CciI	TCATGA	2 cut(s)	1351, 1512
CfoI	GCGC	4 cut(s)	674, 1108, 1760, 2014
Cfr10I	RCCGGY	4 cut(s)	674, 774, 821, 1342
Cfr13I	GGNCC	3 cut(s)	311, 772, 798
CseI	GACGC	1 cut(s)	548
Csp6I	GTAC	4 cut(s)	778, 793, 1060, 1461
CspAI	ACCGGT	2 cut(s)	774, 1342
CviQI	GTAC	4 cut(s)	778, 793, 1060, 1461
DpnI	GATC	9 cut(s)	16, 428, 914, 1020, 1611, 1689, 1788, 2223, 2643
DpnII	GATC	9 cut(s)	14, 426, 912, 1018, 1609, 1687, 1786, 2221, 2641
EaeI	YGGCCR	1 cut(s)	2463
Eam1104I	CTCTTC	2 cut(s)	890, 1151
EarI	CTCTTC	2 cut(s)	890, 1151
EciI	GGCGGA	2 cut(s)	72, 92
Eco130I	CCWWGG	4 cut(s)	485, 940, 2111, 2551
Eco147I	AGGCCT	1 cut(s)	1405
Eco31I	GGTCTC	2 cut(s)	1188, 1219
Eco47I	GGWCC	3 cut(s)	311, 772, 798
Eco47III	AGCGCT	1 cut(s)	2013
Eco57I	CTGAAG	1 cut(s)	2628
EcoO109I	RGGNCCY	1 cut(s)	311
EcoRI	GAATTC	1 cut(s)	1069
EcoRII	CCWGG	3 cut(s)	452, 1179, 2239
EcoT14I	CCWWGG	4 cut(s)	485, 940, 2111, 2551
EcoT22I	ATGCAT	1 cut(s)	1326
ErhI	CCWWGG	4 cut(s)	485, 940, 2111, 2551
Esp3I	CGTCTC	4 cut(s)	150, 566, 1362, 2628
FalI	AAGNNNNNCTT	4 cut(s)	300, 332, 2045, 2077
FaqI	GGGAC	6 cut(s)	156, 261, 785, 1589, 2017, 2148
FauI	CCCGC	1 cut(s)	35
FbaI	TGATCA	2 cut(s)	1018, 2641
FblI	GTMKAC	1 cut(s)	1170
FokI	GGATG	4 cut(s)	825, 2105, 2115, 2297
FspBI	CTAG	4 cut(s)	957, 1596, 1772, 2112
GlaI	GCGC	4 cut(s)	673, 1107, 1759, 2013
GsaI	CCCAGC	1 cut(s)	1444
GsuI	CTGGAG	1 cut(s)	542
HaeII	RGCGCY	1 cut(s)	2015
HaeIII	GGCC	5 cut(s)	461, 1405, 2293, 2465, 2496
HapII	CCGG	6 cut(s)	675, 775, 822, 1343, 2090, 2462
HgaI	GACGC	1 cut(s)	548
HhaI	GCGC	4 cut(s)	674, 1108, 1760, 2014
Hin1I	GRCGYC	1 cut(s)	559
Hin6I	GCGC	4 cut(s)	672, 1106, 1758, 2012
HinP1I	GCGC	4 cut(s)	672, 1106, 1758, 2012
HindIII	AAGCTT	2 cut(s)	903, 2510
HinfI	GANTC	9 cut(s)	291, 570, 1434, 1549, 1802, 1850, 2107, 2528, 2663
HpaII	CCGG	6 cut(s)	675, 775, 822, 1343, 2090, 2462
HphI	GGTGA	9 cut(s)	799, 842, 1013, 1391, 1658, 1988, 2405, 2651, 2672
Hpy166II	GTNNAC	2 cut(s)	1171, 2574
Hpy188III	TCNNGA	8 cut(s)	521, 588, 1100, 1191, 1352, 1513, 1675, 2251
Hpy8I	GTNNAC	2 cut(s)	1171, 2574
HpyCH4III	ACNGT	9 cut(s)	170, 664, 1420, 1460, 1976, 2157, 2203, 2358, 2639
HpyCH4IV	ACGT	4 cut(s)	333, 780, 1371, 1967
HpyF10VI	GCNNNNNNNGC	9 cut(s)	111, 120, 350, 508, 678, 1087, 1103, 1669, 1922
HpySE526I	ACGT	4 cut(s)	333, 780, 1371, 1967
Hsp92I	GRCGYC	1 cut(s)	559
HspAI	GCGC	4 cut(s)	672, 1106, 1758, 2012
KroI	GCCGGC	1 cut(s)	674
KroNI	GCCGGC	1 cut(s)	676
Ksp22I	TGATCA	2 cut(s)	1018, 2641
Kzo9I	GATC	9 cut(s)	14, 426, 912, 1018, 1609, 1687, 1786, 2221, 2641
LguI	GCTCTTC	1 cut(s)	890
LmnI	GCTCC	8 cut(s)	189, 1113, 1334, 1880, 1945, 2072, 2439, 2603
LweI	GCATC	7 cut(s)	1026, 1093, 1140, 1672, 1745, 2137, 2426
MaeI	CTAG	4 cut(s)	957, 1596, 1772, 2112
MaeII	ACGT	4 cut(s)	333, 780, 1371, 1967
MaeIII	GTNAC	7 cut(s)	649, 781, 1397, 1414, 1963, 1981, 2470
MalI	GATC	9 cut(s)	16, 428, 914, 1020, 1611, 1689, 1788, 2223, 2643
MboI	GATC	9 cut(s)	14, 426, 912, 1018, 1609, 1687, 1786, 2221, 2641
MfeI	CAATTG	2 cut(s)	758, 1208
MflI	RGATCY	4 cut(s)	1609, 1687, 1786, 2221
MhlI	GDGCHC	1 cut(s)	2444
MlyI	GAGTC	4 cut(s)	1428, 1859, 2101, 2522
MmeI	TCCRAC	1 cut(s)	1474
Mph1103I	ATGCAT	1 cut(s)	1326
MroNI	GCCGGC	1 cut(s)	674
MroXI	GAANNNNTTC	4 cut(s)	750, 1069, 1236, 2157
MseI	TTAA	8 cut(s)	35, 210, 1472, 1527, 1860, 1953, 2052, 2591
MslI	CAYNNNNRTG	2 cut(s)	1283, 1329
MspA1I	CMGCKG	2 cut(s)	1555, 2375
MspCI	CTTAAG	1 cut(s)	1526
MspI	CCGG	6 cut(s)	675, 775, 822, 1343, 2090, 2462
MspR9I	CCNGG	3 cut(s)	454, 1181, 2241
MunI	CAATTG	2 cut(s)	758, 1208
MvaI	CCWGG	3 cut(s)	454, 1181, 2241
MwoI	GCNNNNNNNGC	9 cut(s)	111, 120, 350, 508, 678, 1087, 1103, 1669, 1922
NaeI	GCCGGC	1 cut(s)	676
NcoI	CCATGG	1 cut(s)	940
NdeII	GATC	9 cut(s)	14, 426, 912, 1018, 1609, 1687, 1786, 2221, 2641
NgoMIV	GCCGGC	1 cut(s)	674
NheI	GCTAGC	1 cut(s)	1595
NlaIV	GGNNCC	4 cut(s)	154, 773, 1007, 2557
NmuCI	GTSAC	4 cut(s)	781, 1414, 1963, 2470
NsiI	ATGCAT	1 cut(s)	1326
NspI	RCATGY	2 cut(s)	1288, 1589
PagI	TCATGA	2 cut(s)	1351, 1512
PaqCI	CACCTGC	1 cut(s)	1032
PasI	CCCWGGG	1 cut(s)	2240
PceI	AGGCCT	1 cut(s)	1405
PciI	ACATGT	1 cut(s)	1284
PciSI	GCTCTTC	1 cut(s)	890
PdiI	GCCGGC	1 cut(s)	676
PdmI	GAANNNNTTC	4 cut(s)	750, 1069, 1236, 2157
PfeI	GAWTC	5 cut(s)	291, 570, 1549, 1802, 2663
PflFI	GACNNNGTC	2 cut(s)	1961, 2411
PflMI	CCANNNNNTGG	1 cut(s)	453
PinAI	ACCGGT	2 cut(s)	774, 1342
PleI	GAGTC	4 cut(s)	1428, 1858, 2101, 2522
PpsI	GAGTC	4 cut(s)	1428, 1858, 2101, 2522
Ppu21I	YACGTR	2 cut(s)	781, 1968
PpuMI	RGGWCCY	1 cut(s)	311
PscI	ACATGT	1 cut(s)	1284
Psp1406I	AACGTT	1 cut(s)	333
Psp5II	RGGWCCY	1 cut(s)	311
Psp6I	CCWGG	3 cut(s)	452, 1179, 2239
PspFI	CCCAGC	1 cut(s)	1440
PspGI	CCWGG	3 cut(s)	452, 1179, 2239
PspN4I	GGNNCC	4 cut(s)	154, 773, 1007, 2557
PspPI	GGNCC	3 cut(s)	311, 772, 798
PspPPI	RGGWCCY	1 cut(s)	311
PsrI	GAACNNNNNNTAC	2 cut(s)	599, 631
PstI	CTGCAG	2 cut(s)	1086, 2011
PstNI	CAGNNNCTG	1 cut(s)	1447
PsuI	RGATCY	4 cut(s)	1609, 1687, 1786, 2221
PsyI	GACNNNGTC	2 cut(s)	1961, 2411
PvuII	CAGCTG	1 cut(s)	2375
RsaI	GTAC	4 cut(s)	779, 794, 1061, 1462
RsaNI	GTAC	4 cut(s)	778, 793, 1060, 1461
RseI	CAYNNNNRTG	2 cut(s)	1283, 1329
SapI	GCTCTTC	1 cut(s)	890
SaqAI	TTAA	8 cut(s)	35, 210, 1472, 1527, 1860, 1953, 2052, 2591
Sau3AI	GATC	9 cut(s)	14, 426, 912, 1018, 1609, 1687, 1786, 2221, 2641
Sau96I	GGNCC	3 cut(s)	311, 772, 798
SchI	GAGTC	4 cut(s)	1428, 1859, 2101, 2522
ScrFI	CCNGG	3 cut(s)	454, 1181, 2241
SduI	GDGCHC	1 cut(s)	2444
SfaNI	GCATC	7 cut(s)	1026, 1093, 1140, 1672, 1745, 2137, 2426
SfcI	CTRYAG	4 cut(s)	610, 1082, 2007, 2421
SinI	GGWCC	3 cut(s)	311, 772, 798
SmiMI	CAYNNNNRTG	2 cut(s)	1283, 1329
SmlI	CTYRAG	1 cut(s)	1526
SmoI	CTYRAG	1 cut(s)	1526
SseBI	AGGCCT	1 cut(s)	1405
SsiI	CCGC	8 cut(s)	42, 57, 77, 190, 550, 1553, 1599, 2024
SspI	AATATT	1 cut(s)	1235
SspMI	CTAG	4 cut(s)	957, 1596, 1772, 2112
StuI	AGGCCT	1 cut(s)	1405
StyD4I	CCNGG	3 cut(s)	452, 1179, 2239
StyI	CCWWGG	4 cut(s)	485, 940, 2111, 2551
TaaI	ACNGT	9 cut(s)	170, 664, 1420, 1460, 1976, 2157, 2203, 2358, 2639
TaiI	ACGT	4 cut(s)	336, 783, 1374, 1970
TaqI	TCGA	8 cut(s)	29, 89, 163, 357, 628, 952, 1101, 1123
TatI	WGTACW	2 cut(s)	792, 1059
TfiI	GAWTC	5 cut(s)	291, 570, 1549, 1802, 2663
Tru1I	TTAA	8 cut(s)	35, 210, 1472, 1527, 1860, 1953, 2052, 2591
Tru9I	TTAA	8 cut(s)	35, 210, 1472, 1527, 1860, 1953, 2052, 2591
TscAI	CASTG	7 cut(s)	163, 268, 580, 1285, 1760, 1992, 2361
TseFI	GTSAC	4 cut(s)	781, 1414, 1963, 2470
Tsp45I	GTSAC	4 cut(s)	781, 1414, 1963, 2470
TspGWI	ACGGA	2 cut(s)	1893, 2616
TspRI	CASTG	7 cut(s)	163, 268, 580, 1285, 1760, 1992, 2361
Tth111I	GACNNNGTC	2 cut(s)	1961, 2411
Van91I	CCANNNNNTGG	1 cut(s)	453
Vha464I	CTTAAG	1 cut(s)	1526
VpaK11BI	GGWCC	3 cut(s)	311, 772, 798
XapI	RAATTY	7 cut(s)	284, 847, 1069, 1566, 1891, 1909, 2564
XceI	RCATGY	2 cut(s)	1288, 1589
XcmI	CCANNNNNNNNNTGG	2 cut(s)	454, 723
XmaJI	CCTAGG	1 cut(s)	2111
XmiI	GTMKAC	1 cut(s)	1170
XmnI	GAANNNNTTC	4 cut(s)	750, 1069, 1236, 2157
XspI	CTAG	4 cut(s)	957, 1596, 1772, 2112
Zsp2I	ATGCAT	1 cut(s)	1326

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.