AT4G32650

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009077
Physical Location & Seq
Reverse (-)
15751271 .. 15755047
3777 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32650.3

Sequence Viewer

Length: 1989 bp
ATGTCTACGACGACTACTGAGGCGAGATCACCGTTGCCGTTGTTGTTGAGAAGAGGAAGGTCTTCGACGGCGTTATCGGCGTCGACGGCGGAAGCAAGATCGCCGTTATCGATACTGCAGTTCAGAAGAAGATCGAGCAAAGATGTGAGGAACATAACGTCAGTTTCGAGCAGTCTCTTGCCTGCTTTTGGGACTTTCATCGAAGATGATAATCCTTCTTCCAAACCTTTCATCGTTCTTCACTTTGATCGTCGTTACAGGTTGTGGGAATTGTTTTTGGTGATATTGGTTGGGTATTCGGCGTGGGCATCTTTGTTCGAGTTGGCTTTCGAGAAAGCTGCCGAAGGAGCGTTATTGACCATTGATCTCGTCGTTGACTTCTTCTTCGCCGTTGATATCATCCTCACCTTTTTTGTTTCCTACTTGGATAATACTACTTACCTCAATGTCACCGACCACAAGCTCATCGCCAAACGGTACTTGAAGAGCGTGGCTTTTGTGATGGACGTAGCATCAACGTTACCCATTCAATTCATTTATAAAACTATTACCGGAGATGTCGGACGAGGCCAAGCTTTCGGCTTCCTTAATTTACTCCGCCTCTGGCGTCTCCGTCGTGTAGCCGAACTCTTTAAAAGACTAGAGAAAGACGCACATTTCAACTATTTCGTGATCCGAGTCATCAAACTTCTATGTGTAACGATATTTTGGATACATTTGGCGGGTTGCATTTTATACTGGATAGCCTACCATTATCCAAGGCCTACGGATACATGGATAGGATCGCAAGTTGAGGATTTTAAGGAAAGAAGTGTATGGTTAGGGTACACTTACTCAATGTACTGGTCCATTGTCACACTCACTACCGTGGGTTACGGTGATTTGCATGCAGTTAATAGCCGTGAGAAGACATTCAACATGTTCTACATGCTTTTCAACATTGGCCTCACTTCTTATATCATCGGTATCATGACCAATCTAGTTGTCCATGGCGCTCTTCGTACATTCGCCATGAGGAGTGCGATCAATGATATATTGCGATACACAAGCAAGAACAGGTTACCGGATACAATGAGGGAACAGATGCTTGCACATATGCAGCTCAAGTTCAAGACCGCGGAGTTAAGGCAAGAAGAGGTTCTTCAAGACTTACCTAAGGCCATAAGATCAAGCATTAACCAACATCTATTCCGCTCCATCATCGAAGAAGCTTATCTTTTTAAAGGATTCCCCGAAGGCCTCCTCGTCCAGCTGGTTTCGCAAATACAAGCAGAATATTTTCCGCCGAAAATGGAGATAATCTTGCAGAATGAGATTCCAACGGATTTCTACGTAATTGTATCTGGAGGAGTGGATATAATTGCTTCCAAGGGTGTGAGTGAACAGGTATTAGCGAAGTTAGGTCCCGGAAGTATGGCAGGAGAGATAGGAGTAGTGTTCAACATTCCTCAGCCTTTCACAGTGAGGACACGTCGACTTTCACAAGTTATCAGAATTGGTCATCATAAGTTCAAAGAAATGGTGCAGTCTGATAATGACGTCGACGCCAAAATGATCATTGCCAATTTCATGACATATCTTAAGGGATTGAATGATGAGTTAAAAAAAGAAATTCCTTTTCTTAGAGATTTATTAGATGACGCAGATGCTCAGGTTCAGGAAACAGTTCAGTCAGAGGAAACACCACAAAGTAACGATGAGGAAATAGTTACGGTCTCAAGACATGAAAATGGACAGATAGAAGAGAGAAGAAGAGAGGGAGTTCCAAAAAGAGTGATAATTCATGGACAAGCTCCTCCTAATCAAGATAACAAAAACAATGGTGATTCCAACGGTAGACTTATCATTTTACCTGACTCTATCCAACTTCTATTCGACTTAGCTGAGAAGAAGTTGGGGAAACGAGGAAGCACGATTGCAATGGCAGATGGTGCACATGTTGAACAAATTGATGCTCTTCGAGAAAACGATCATTTATATATTTTCTAA

Protein Analysis

662

Amino Acids

75.6

Weight (kDa)

7.28

Isoelectric Point (pI)

50.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 86 - 330 1.4e-41 Ion transport protein
Ion_trans_2 PF07885 273 - 328 9.1e-12 Ion channel
cNMP_binding PF00027 426 - 511 5.1e-13 Cyclic nucleotide-binding domain
KHA PF11834 589 - 660 1.4e-20 KHA, dimerisation domain of potassium ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
AatII GACGTC 1 cut(s) 1542
AccBSI CCGCTC 1 cut(s) 1194
AccI GTMKAC 5 cut(s) 5, 83, 1474, 1542, 1837
AccII CGCG 1 cut(s) 1118
AciI CCGC 7 cut(s) 89, 598, 722, 1116, 1118, 1192, 1283
AclI AACGTT 1 cut(s) 518
AclWI GGATC 2 cut(s) 667, 790
AcsI RAATTY 1 cut(s) 1611
AcyI GRCGYC 4 cut(s) 80, 607, 1539, 1545
AfaI GTAC 4 cut(s) 479, 827, 842, 1003
AfiI CCNNNNNNNGG 1 cut(s) 188
AflII CTTAAG 1 cut(s) 1580
AflIII ACRYGT 3 cut(s) 918, 1469, 1936
AjiI CACGTC 1 cut(s) 1472
AleI CACNNNNGTG 1 cut(s) 866
AluBI AGCT 8 cut(s) 338, 463, 575, 1102, 1211, 1252, 1793, 1883
AluI AGCT 8 cut(s) 338, 463, 575, 1102, 1211, 1252, 1793, 1883
Alw21I GWGCWC 1 cut(s) 1936
Alw26I GTCTC 3 cut(s) 179, 614, 1720
Alw44I GTGCAC 1 cut(s) 1932
AlwI GGATC 2 cut(s) 667, 790
AoxI GGCC 5 cut(s) 568, 761, 943, 1158, 1237
ApaLI GTGCAC 1 cut(s) 1932
ApeKI GCWGC 2 cut(s) 338, 1099
ApoI RAATTY 1 cut(s) 1611
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
Asp700I GAANNNNTTC 5 cut(s) 61, 911, 1137, 1278, 1665
AspLEI GCGC 1 cut(s) 995
AspS9I GGNCC 2 cut(s) 846, 1403
AsuC2I CCSGG 1 cut(s) 1407
AsuHPI GGTGA 6 cut(s) 21, 292, 397, 442, 890, 1835
AvaII GGWCC 2 cut(s) 846, 1403
AxyI CCTNAGG 1 cut(s) 1155
BaeGI GKGCMC 1 cut(s) 1936
BaeI ACNNNNGTAYC 2 cut(s) 104, 137
BbsI GAAGAC 2 cut(s) 54, 914
Bbv12I GWGCWC 1 cut(s) 1936
BbvCI CCTCAGC 1 cut(s) 1449
BbvI GCAGC 2 cut(s) 325, 1111
BccI CCATC 3 cut(s) 496, 1205, 1922
BceAI ACGGC 6 cut(s) 22, 84, 88, 102, 374, 885
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BciVI GTATCC 3 cut(s) 705, 763, 1060
BclI TGATCA 1 cut(s) 1554
BcnI CCSGG 1 cut(s) 1407
BcoDI GTCTC 3 cut(s) 179, 614, 1720
BfaI CTAG 2 cut(s) 641, 980
BfmI CTRYAG 1 cut(s) 116
BfoI RGCGCY 1 cut(s) 996
BfrI CTTAAG 1 cut(s) 1580
BfuI GTATCC 3 cut(s) 705, 763, 1060
BisI GCNGC 2 cut(s) 339, 1100
BlsI GCNGC 2 cut(s) 340, 1101
Bme1390I CCNGG 1 cut(s) 1407
Bme18I GGWCC 2 cut(s) 846, 1403
BmgBI CACGTC 1 cut(s) 1472
BmgT120I GGNCC 2 cut(s) 846, 1403
BmiI GGNNCC 1 cut(s) 1405
BmrFI CCNGG 1 cut(s) 1407
BmsI GCATC 5 cut(s) 317, 521, 1074, 1636, 1942
BpiI GAAGAC 2 cut(s) 54, 914
BpmI CTGGAG 1 cut(s) 1365
Bpu10I CCTNAGC 2 cut(s) 1449, 1650
BpuEI CTTGAG 2 cut(s) 1088, 1702
BpuMI CCSGG 1 cut(s) 1407
Bsa29I ATCGAT 1 cut(s) 110
BsaAI YACGTR 1 cut(s) 1333
BsaBI GATNNNNATC 1 cut(s) 210
BsaHI GRCGYC 4 cut(s) 80, 607, 1539, 1545
BsaI GGTCTC 1 cut(s) 1720
BsaJI CCNNGG 5 cut(s) 758, 867, 988, 1116, 1368
BsaWI WCCGGW 2 cut(s) 551, 1063
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse1I ACTGG 2 cut(s) 743, 848
Bse21I CCTNAGG 1 cut(s) 1155
Bse3DI GCAATG 2 cut(s) 1557, 1926
Bse8I GATNNNNATC 1 cut(s) 210
BseCI ATCGAT 1 cut(s) 110
BseDI CCNNGG 5 cut(s) 758, 867, 988, 1116, 1368
BseGI GGATG 1 cut(s) 399
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMI GCAATG 2 cut(s) 1557, 1926
BseMII CTCAG 4 cut(s) 9, 1463, 1664, 1875
BseNI ACTGG 2 cut(s) 743, 848
BseRI GAGGAG 4 cut(s) 1030, 1232, 1362, 1785
BseSI GKGCMC 1 cut(s) 1936
BseXI GCAGC 2 cut(s) 325, 1111
BsgI GTGCAG 1 cut(s) 1544
Bsh1236I CGCG 1 cut(s) 1118
BshFI GGCC 5 cut(s) 570, 763, 945, 1160, 1239
BshVI ATCGAT 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 1936
BsiSI CCGG 3 cut(s) 552, 1064, 1407
BslFI GGGAC 2 cut(s) 205, 1389
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 3 cut(s) 179, 614, 1720
BsmBI CGTCTC 1 cut(s) 614
BsmFI GGGAC 2 cut(s) 205, 1389
BsnI GGCC 5 cut(s) 570, 763, 945, 1160, 1239
Bso31I GGTCTC 1 cut(s) 1720
Bsp1286I GDGCHC 1 cut(s) 1936
Bsp19I CCATGG 1 cut(s) 988
BspACI CCGC 7 cut(s) 89, 598, 722, 1116, 1118, 1192, 1283
BspANI GGCC 5 cut(s) 570, 763, 945, 1160, 1239
BspCNI CTCAG 4 cut(s) 10, 1462, 1663, 1876
BspDI ATCGAT 1 cut(s) 110
BspFNI CGCG 1 cut(s) 1118
BspHI TCATGA 2 cut(s) 969, 1569
BspLI GGNNCC 1 cut(s) 1405
BspMAI CTGCAG 1 cut(s) 120
BspPI GGATC 2 cut(s) 667, 790
BspQI GCTCTTC 3 cut(s) 479, 1002, 1962
BspTI CTTAAG 1 cut(s) 1580
BspTNI GGTCTC 1 cut(s) 1720
BsrBI CCGCTC 1 cut(s) 1194
BsrDI GCAATG 2 cut(s) 1557, 1926
BsrI ACTGG 2 cut(s) 743, 848
BssECI CCNNGG 5 cut(s) 758, 867, 988, 1116, 1368
BssNI GRCGYC 4 cut(s) 80, 607, 1539, 1545
BssT1I CCWWGG 3 cut(s) 758, 988, 1368
Bst4CI ACNGT 8 cut(s) 33, 477, 868, 878, 1462, 1666, 1714, 1835
Bst6I CTCTTC 7 cut(s) 46, 479, 1002, 1128, 1737, 1747, 1962
BstACI GRCGYC 4 cut(s) 80, 607, 1539, 1545
BstAFI CTTAAG 1 cut(s) 1580
BstAPI GCANNNNNTGC 1 cut(s) 1931
BstBAI YACGTR 1 cut(s) 1333
BstC8I GCNNGC 3 cut(s) 183, 888, 1089
BstDEI CTNAG 7 cut(s) 18, 1155, 1449, 1622, 1650, 1879, 1884
BstDSI CCRYGG 3 cut(s) 867, 988, 1116
BstEII GGTNACC 1 cut(s) 1059
BstF5I GGATG 1 cut(s) 399
BstFNI CGCG 1 cut(s) 1118
BstH2I RGCGCY 1 cut(s) 996
BstHHI GCGC 1 cut(s) 995
BstMAI GTCTC 3 cut(s) 179, 614, 1720
BstMWI GCNNNNNNNGC 5 cut(s) 77, 86, 347, 1258, 1931
BstNSI RCATGY 4 cut(s) 890, 922, 931, 1940
BstPI GGTNACC 1 cut(s) 1059
BstSCI CCNGG 1 cut(s) 1405
BstSFI CTRYAG 1 cut(s) 116
BstSLI GKGCMC 1 cut(s) 1936
BstSNI TACGTA 1 cut(s) 1333
BstUI CGCG 1 cut(s) 1118
BstV1I GCAGC 2 cut(s) 325, 1111
BstV2I GAAGAC 2 cut(s) 54, 914
Bsu15I ATCGAT 1 cut(s) 110
Bsu36I CCTNAGG 1 cut(s) 1155
BsuI GTATCC 3 cut(s) 705, 763, 1060
BsuRI GGCC 5 cut(s) 570, 763, 945, 1160, 1239
BsuTUI ATCGAT 1 cut(s) 110
BtgI CCRYGG 3 cut(s) 867, 988, 1116
BtgZI GCGATG 1 cut(s) 451
BtrI CACGTC 1 cut(s) 1472
BtsCI GGATG 1 cut(s) 399
BtsIMutI CAGTG 1 cut(s) 1467
Cac8I GCNNGC 3 cut(s) 183, 888, 1089
CciI TCATGA 2 cut(s) 969, 1569
CfoI GCGC 1 cut(s) 995
Cfr13I GGNCC 2 cut(s) 846, 1403
Cfr42I CCGCGG 1 cut(s) 1119
ClaI ATCGAT 1 cut(s) 110
CseI GACGC 5 cut(s) 69, 596, 659, 1553, 1649
Csp6I GTAC 4 cut(s) 478, 826, 841, 1002
CviQI GTAC 4 cut(s) 478, 826, 841, 1002
DdeI CTNAG 7 cut(s) 18, 1155, 1449, 1622, 1650, 1879, 1884
DraI TTTAAA 2 cut(s) 634, 1222
Eam1104I CTCTTC 7 cut(s) 46, 479, 1002, 1128, 1737, 1747, 1962
EarI CTCTTC 7 cut(s) 46, 479, 1002, 1128, 1737, 1747, 1962
EciI GGCGGA 3 cut(s) 104, 587, 1272
Eco105I TACGTA 1 cut(s) 1333
Eco130I CCWWGG 3 cut(s) 758, 988, 1368
Eco147I AGGCCT 2 cut(s) 763, 1239
Eco31I GGTCTC 1 cut(s) 1720
Eco32I GATATC 1 cut(s) 397
Eco47I GGWCC 2 cut(s) 846, 1403
Eco81I CCTNAGG 1 cut(s) 1155
Eco91I GGTNACC 1 cut(s) 1059
EcoO109I RGGNCCY 1 cut(s) 1403
EcoO65I GGTNACC 1 cut(s) 1059
EcoRV GATATC 1 cut(s) 397
EcoT14I CCWWGG 3 cut(s) 758, 988, 1368
ErhI CCWWGG 3 cut(s) 758, 988, 1368
Esp3I CGTCTC 1 cut(s) 614
FalI AAGNNNNNCTT 4 cut(s) 1125, 1157, 1200, 1232
FaqI GGGAC 2 cut(s) 205, 1389
FauI CCCGC 1 cut(s) 715
FauNDI CATATG 1 cut(s) 1095
FbaI TGATCA 1 cut(s) 1554
FblI GTMKAC 5 cut(s) 5, 83, 1474, 1542, 1837
Fnu4HI GCNGC 2 cut(s) 339, 1100
FokI GGATG 1 cut(s) 386
Fsp4HI GCNGC 2 cut(s) 339, 1100
FspBI CTAG 2 cut(s) 641, 980
GlaI GCGC 1 cut(s) 994
GluI GCNGC 2 cut(s) 339, 1100
GsuI CTGGAG 1 cut(s) 1365
HaeII RGCGCY 1 cut(s) 996
HaeIII GGCC 5 cut(s) 570, 763, 945, 1160, 1239
HapII CCGG 3 cut(s) 552, 1064, 1407
HgaI GACGC 5 cut(s) 69, 596, 659, 1553, 1649
HhaI GCGC 1 cut(s) 995
Hin1I GRCGYC 4 cut(s) 80, 607, 1539, 1545
Hin6I GCGC 1 cut(s) 993
HinP1I GCGC 1 cut(s) 993
HincII GTYRAC 4 cut(s) 84, 376, 1475, 1543
HindII GTYRAC 4 cut(s) 84, 376, 1475, 1543
HindIII AAGCTT 2 cut(s) 573, 1209
HinfI GANTC 5 cut(s) 678, 1227, 1315, 1826, 1856
HpaII CCGG 3 cut(s) 552, 1064, 1407
HphI GGTGA 6 cut(s) 21, 292, 397, 442, 890, 1835
Hpy166II GTNNAC 9 cut(s) 6, 84, 376, 828, 1382, 1475, 1543, 1838, 1934
Hpy188I TCNGA 6 cut(s) 125, 563, 677, 1493, 1531, 1675
Hpy8I GTNNAC 9 cut(s) 6, 84, 376, 828, 1382, 1475, 1543, 1838, 1934
HpyAV CCTTC 4 cut(s) 51, 225, 338, 1229
HpyCH4III ACNGT 8 cut(s) 33, 477, 868, 878, 1462, 1666, 1714, 1835
HpyCH4IV ACGT 6 cut(s) 158, 507, 518, 1332, 1471, 1539
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 86, 347, 1258, 1931
HpyF3I CTNAG 7 cut(s) 18, 1155, 1449, 1622, 1650, 1879, 1884
HpySE526I ACGT 6 cut(s) 158, 507, 518, 1332, 1471, 1539
Hsp92I GRCGYC 4 cut(s) 80, 607, 1539, 1545
HspAI GCGC 1 cut(s) 993
Ksp22I TGATCA 1 cut(s) 1554
KspI CCGCGG 1 cut(s) 1119
LguI GCTCTTC 3 cut(s) 479, 1002, 1962
LmnI GCTCC 3 cut(s) 347, 1199, 1798
Lsp1109I GCAGC 2 cut(s) 325, 1111
LweI GCATC 5 cut(s) 317, 521, 1074, 1636, 1942
MaeI CTAG 2 cut(s) 641, 980
MaeII ACGT 6 cut(s) 158, 507, 518, 1332, 1471, 1539
MaeIII GTNAC 9 cut(s) 254, 448, 519, 697, 853, 872, 1059, 1691, 1708
MbiI CCGCTC 1 cut(s) 1194
MhlI GDGCHC 1 cut(s) 1936
MlyI GAGTC 2 cut(s) 687, 1850
MmeI TCCRAC 4 cut(s) 541, 1343, 1854, 1888
MroXI GAANNNNTTC 5 cut(s) 61, 911, 1137, 1278, 1665
MseI TTAA 9 cut(s) 588, 633, 801, 894, 1124, 1176, 1221, 1581, 1601
MslI CAYNNNNRTG 2 cut(s) 866, 1728
MspA1I CMGCKG 2 cut(s) 1118, 1252
MspCI CTTAAG 1 cut(s) 1580
MspI CCGG 3 cut(s) 552, 1064, 1407
MspR9I CCNGG 1 cut(s) 1407
MvnI CGCG 1 cut(s) 1118
MwoI GCNNNNNNNGC 5 cut(s) 77, 86, 347, 1258, 1931
NciI CCSGG 1 cut(s) 1407
NcoI CCATGG 1 cut(s) 988
NdeI CATATG 1 cut(s) 1095
NlaIV GGNNCC 1 cut(s) 1405
NmuCI GTSAC 2 cut(s) 448, 853
NspI RCATGY 4 cut(s) 890, 922, 931, 1940
OliI CACNNNNGTG 1 cut(s) 866
PaeI GCATGC 1 cut(s) 890
PagI TCATGA 2 cut(s) 969, 1569
PceI AGGCCT 2 cut(s) 763, 1239
PciI ACATGT 2 cut(s) 918, 1936
PciSI GCTCTTC 3 cut(s) 479, 1002, 1962
PcsI WCGNNNNNNNCGW 2 cut(s) 107, 164
PdmI GAANNNNTTC 5 cut(s) 61, 911, 1137, 1278, 1665
PfeI GAWTC 3 cut(s) 1227, 1315, 1826
PfoI TCCNGGA 1 cut(s) 1405
PkrI GCNGC 2 cut(s) 340, 1101
PleI GAGTC 2 cut(s) 686, 1850
PpsI GAGTC 2 cut(s) 686, 1850
Ppu21I YACGTR 1 cut(s) 1333
PpuMI RGGWCCY 1 cut(s) 1403
PscI ACATGT 2 cut(s) 918, 1936
PsiI TTATAA 1 cut(s) 540
Psp1406I AACGTT 1 cut(s) 518
Psp5II RGGWCCY 1 cut(s) 1403
PspEI GGTNACC 1 cut(s) 1059
PspN4I GGNNCC 1 cut(s) 1405
PspPI GGNCC 2 cut(s) 846, 1403
PspPPI RGGWCCY 1 cut(s) 1403
PstI CTGCAG 1 cut(s) 120
PvuII CAGCTG 1 cut(s) 1252
RsaI GTAC 4 cut(s) 479, 827, 842, 1003
RsaNI GTAC 4 cut(s) 478, 826, 841, 1002
RseI CAYNNNNRTG 2 cut(s) 866, 1728
SacII CCGCGG 1 cut(s) 1119
SalI GTCGAC 3 cut(s) 82, 1473, 1541
SapI GCTCTTC 3 cut(s) 479, 1002, 1962
SaqAI TTAA 9 cut(s) 588, 633, 801, 894, 1124, 1176, 1221, 1581, 1601
SatI GCNGC 2 cut(s) 339, 1100
Sau96I GGNCC 2 cut(s) 846, 1403
SchI GAGTC 2 cut(s) 687, 1850
ScrFI CCNGG 1 cut(s) 1407
SduI GDGCHC 1 cut(s) 1936
SfaNI GCATC 5 cut(s) 317, 521, 1074, 1636, 1942
SfcI CTRYAG 1 cut(s) 116
Sfr303I CCGCGG 1 cut(s) 1119
SgrBI CCGCGG 1 cut(s) 1119
SgrDI CGTCGACG 2 cut(s) 82, 1541
SinI GGWCC 2 cut(s) 846, 1403
SmiMI CAYNNNNRTG 2 cut(s) 866, 1728
SmlI CTYRAG 3 cut(s) 1103, 1580, 1717
SmoI CTYRAG 3 cut(s) 1103, 1580, 1717
SnaBI TACGTA 1 cut(s) 1333
SphI GCATGC 1 cut(s) 890
SseBI AGGCCT 2 cut(s) 763, 1239
SsiI CCGC 7 cut(s) 89, 598, 722, 1116, 1118, 1192, 1283
SspI AATATT 1 cut(s) 1277
SspMI CTAG 2 cut(s) 641, 980
StuI AGGCCT 2 cut(s) 763, 1239
StyD4I CCNGG 1 cut(s) 1405
StyI CCWWGG 3 cut(s) 758, 988, 1368
TaaI ACNGT 8 cut(s) 33, 477, 868, 878, 1462, 1666, 1714, 1835
TaiI ACGT 6 cut(s) 161, 510, 521, 1335, 1474, 1542
TatI WGTACW 1 cut(s) 840
TfiI GAWTC 3 cut(s) 1227, 1315, 1826
Tru1I TTAA 9 cut(s) 588, 633, 801, 894, 1124, 1176, 1221, 1581, 1601
Tru9I TTAA 9 cut(s) 588, 633, 801, 894, 1124, 1176, 1221, 1581, 1601
TscAI CASTG 1 cut(s) 1467
TseFI GTSAC 2 cut(s) 448, 853
TseI GCWGC 2 cut(s) 338, 1099
Tsp45I GTSAC 2 cut(s) 448, 853
TspDTI ATGAA 6 cut(s) 187, 220, 523, 1558, 1740, 1772
TspGWI ACGGA 3 cut(s) 602, 782, 1337
TspRI CASTG 1 cut(s) 1467
Vha464I CTTAAG 1 cut(s) 1580
VneI GTGCAC 1 cut(s) 1932
VpaK11BI GGWCC 2 cut(s) 846, 1403
XapI RAATTY 1 cut(s) 1611
XceI RCATGY 4 cut(s) 890, 922, 931, 1940
XmiI GTMKAC 5 cut(s) 5, 83, 1474, 1542, 1837
XmnI GAANNNNTTC 5 cut(s) 61, 911, 1137, 1278, 1665
XspI CTAG 2 cut(s) 641, 980
ZraI GACGTC 1 cut(s) 1540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.