Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0009077

Physical Location & Seq

Genomic Coordinates Forward (+)

67844705 .. 67849943

Gene Structure 5239 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G044710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGTCGTCGTCATTCATAACGGCGTCGCCCCTGCCTTTTCTGCTTCGAAGGCGCTCGAGTGGCGAAATGAGGAATTTGGCCTCGGTTTCGAGCAGCATATTGCCCGCTTTTGGAACCGTCATCGATGACGGCCACTTGCAGACCAAGAAATTTGTTGTTGCTCCCTACGATAGGAGATATCGGTGGTGGCAGACGTTCTTGGTGGTGCTGGTGGTGTACTCAGCATGGGCGTCGCCGTTCGAGTTGGCCTTCAAAAAGGTGGCAACAGGGTCTCTCATGCCGGTTGATTTGGTGGTCGATGCCTTCTTCGCCGTTGATATAGTTCTCACTTTCTTTGTGGCTTACTTGGATAAGTCAACCTACCTCCTAGTACTTGATCACAAAAAAATTGCATCCAGGTATGTTACGAGATATTACTTCGCAATGGACGTAGCCTCAACTGTACCGTTTCAATCCCTTCACAGGCTCTTCACTGGGCATACGCACAATGGCCTGGTCTTTGGCATCCTCAATCTGCTTCGTCTCTGGCGTCTCCGGCGCGTCAGTGAACTCTTTAAAAGGCTAGAGAAAGACACTCGCTTTAGTTACTTTTGGATCAGATACGTCAAACTCATATGTGTTACACTATTTGCTGTGCACTCGGCGGGTTGCTTCTACTACTGGATGGCCACCCATCACAAAACCTCAGATGATACATGGATTGGAACTGAGGTCCATGATTTTGAGCACAGAAGCATCTGGTTAGGTTACACATATGCCATATACTGGGCAACTGTCACCCTCACAACTGTTGGCTATGGTGACTTGCACGCAGTTAACTTTGGGGAGAAGGTTTTCAGCATATTCTATATGCTTTTCAACATTGGACTCTTTGCTTATTTGGTTGGAAACATGACCAATCTCGTTGTCCACAGTGCTGTCCGGACATTGTTCATGAGGGACTCCATCAATGAGATATTCAGATATGCAAGCAAGAACAGACTTCCAGAAGGCTTGAAAGAGCAAATGCTTGCCCACATGCAGCTCAAGTTCAAGACAGCAGAGTTACAGCAGGAAGAAGTTCTACAGGACCTTCCGAAAGCAATAAGATCCAGCATTGCCCACCATCTTTTCCGTAAAACCATTGATAAAACATACCTATTCAAAGGAATCTCTGAAGAGCTTACTGTCCAATTGGTCTCAGAGATGAAAGCAGAATATTTTCCACCCAAAGTCGATATTATCTTGCAAAACGAAATTCCAACAGACTTTTACATTCTAGTAGCTGGAGCTTTGGACGTGCTAATATACAAGAACGGCACGGAACAGTTCTTGTCAAATCTAGGATCTGCAGACATGGTAGGAGAGTTTGGCGTATTTTTCAATATCCCACAACCTTTTACAGTGAGAACAAAAAGGCTTTCTCAGGTTATCCGCATCAGTCATCAGTATTTCAAGCAAATGATCCAGCCACAGAATGAAGACGCAAAGATAATCATCACAAACTTTACTCAGCACTTGAAGGACTTAAAACAGGAGGTGTTACAAGAAATTCCATTTCTACCAGAATTGCTGGAAGAACTGAATACAGAGCAGAGTACACATAATCATGAACCAGTGAATTACCATGGAAATCCAACTGTGGAAGGAACAAACAAAACTTGTGCTCCAGGGCCTATCACCATGCGAGTCATAATTCATGGCCATCATCCAAATGAAGGCGACTCGAAAGGAAAACTTATTCATCTGCCGGATTCAATAGACGACCTTCTGAGATTAGCAGAGAAAAAGTTGGAAAAAAGAGGAAGCACAATTGTCATGGCCGACAGCTCAGCGGTTGAAGAACTGAATGCTTTGAGAGACAATGATCACTTGTTTATCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009605 GO:0009607 GO:0009624 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ion_trans KHA cNMP_binding

Protein Analysis

621

Amino Acids

71.5

Weight (kDa)

7.79

Isoelectric Point (pI)

42.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	60 - 304	2.4e-38	Ion transport protein
Ion_trans_2	PF07885	234 - 302	1e-13	Ion channel
cNMP_binding	PF00027	400 - 483	2e-13	Cyclic nucleotide-binding domain
KHA	PF11834	556 - 620	8.3e-21	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009077)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32650 AT4G32650 AT4G32650
fragaria_vesca	FvH4_2g30280 FvH4_2g30280
malus_domestica	MD08G1134700.v1.1 MD15G1113600.v1.1
prunus_persica	Prupe.1G464600_v2.0.a1 Prupe.1G464600_v2.0.a1
pyrus_communis	pycom08g11370
rosa_chinensis	RchiOBHm_Chr6g0312591
rosa_laevigata	RLG00000010313
rosa_multiflora	Rmu_co8350831.1_g000001 Rmu_sc0003094.1_g000003 Rmu_sc0008363.1_g000002
rosa_roxburghii	Rroxscaffold_7G00156670
rosa_rugosa	Rorug06G0396700
rosa_samantha	Rh6AG514600 Rh6BG525400 Rh6CG530600 Rh6DG516700
rosa_wichuraiana	Rw6G044710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	765
AccII	CGCG	1 cut(s)	540
AccIII	TCCGGA	1 cut(s)	921
AciI	CCGC	4 cut(s)	105, 644, 1414, 1814
AclWI	GGATC	4 cut(s)	602, 1083, 1333, 1438
AcoI	YGGCCR	4 cut(s)	130, 666, 1681, 1800
AcsI	RAATTY	4 cut(s)	73, 149, 1236, 1530
AcuI	CTGAAG	1 cut(s)	1176
AcyI	GRCGYC	3 cut(s)	23, 230, 529
AfaI	GTAC	4 cut(s)	218, 372, 444, 1579
AfiI	CCNNNNNNNGG	5 cut(s)	110, 171, 462, 765, 1697
AjiI	CACGTC	1 cut(s)	1279
AjnI	CCWGG	3 cut(s)	395, 492, 1648
AluBI	AGCT	5 cut(s)	1024, 1162, 1265, 1271, 1809
AluI	AGCT	5 cut(s)	1024, 1162, 1265, 1271, 1809
Alw21I	GWGCWC	3 cut(s)	639, 729, 1648
Alw26I	GTCTC	5 cut(s)	276, 527, 536, 1183, 1833
Alw44I	GTGCAC	1 cut(s)	635
AlwI	GGATC	4 cut(s)	602, 1083, 1333, 1438
Ama87I	CYCGRG	1 cut(s)	55
Aor13HI	TCCGGA	1 cut(s)	921
AoxI	GGCC	8 cut(s)	78, 130, 246, 490, 666, 1652, 1681, 1800
ApaLI	GTGCAC	1 cut(s)	635
ApeKI	GCWGC	2 cut(s)	93, 1021
ApoI	RAATTY	4 cut(s)	73, 149, 1236, 1530
Asp700I	GAANNNNTTC	3 cut(s)	833, 1059, 1200
AspLEI	GCGC	2 cut(s)	54, 540
AspS9I	GGNCC	3 cut(s)	712, 1069, 1652
AsuHPI	GGTGA	3 cut(s)	769, 812, 1651
AsuII	TTCGAA	1 cut(s)	46
AvaI	CYCGRG	1 cut(s)	55
AvaII	GGWCC	2 cut(s)	712, 1069
BaeGI	GKGCMC	1 cut(s)	639
BalI	TGGCCA	2 cut(s)	668, 1683
BarI	GAAGNNNNNNTAC	2 cut(s)	1047, 1079
BbsI	GAAGAC	1 cut(s)	1467
Bbv12I	GWGCWC	3 cut(s)	639, 729, 1648
BbvI	GCAGC	2 cut(s)	105, 1033
BccI	CCATC	5 cut(s)	658, 681, 953, 1113, 1692
BceAI	ACGGC	5 cut(s)	36, 145, 220, 296, 1312
BcgI	CGANNNNNNTGC	2 cut(s)	213, 247
BciT130I	CCWGG	3 cut(s)	397, 494, 1650
BclI	TGATCA	2 cut(s)	376, 1846
BcoDI	GTCTC	5 cut(s)	276, 527, 536, 1183, 1833
BfaI	CTAG	4 cut(s)	368, 563, 1259, 1322
BfmI	CTRYAG	2 cut(s)	1064, 1329
BfoI	RGCGCY	1 cut(s)	55
BisI	GCNGC	2 cut(s)	94, 1022
BlpI	GCTNAGC	1 cut(s)	1810
BlsI	GCNGC	2 cut(s)	95, 1023
BmcAI	AGTACT	1 cut(s)	372
Bme1390I	CCNGG	3 cut(s)	397, 494, 1650
Bme18I	GGWCC	2 cut(s)	712, 1069
BmeT110I	CYCGRG	1 cut(s)	55
BmgBI	CACGTC	1 cut(s)	1279
BmgT120I	GGNCC	3 cut(s)	712, 1069, 1652
BmiI	GGNNCC	1 cut(s)	115
BmrFI	CCNGG	3 cut(s)	397, 494, 1650
BmrI	ACTGGG	2 cut(s)	483, 775
BmsI	GCATC	5 cut(s)	289, 401, 513, 744, 1425
BmuI	ACTGGG	2 cut(s)	483, 775
BpiI	GAAGAC	1 cut(s)	1467
BpmI	CTGGAG	2 cut(s)	1287, 1632
Bpu1102I	GCTNAGC	1 cut(s)	1810
Bpu14I	TTCGAA	1 cut(s)	46
BpuEI	CTTGAG	1 cut(s)	1010
Bsa29I	ATCGAT	1 cut(s)	123
BsaBI	GATNNNNATC	1 cut(s)	1475
BsaHI	GRCGYC	3 cut(s)	23, 230, 529
BsaI	GGTCTC	2 cut(s)	276, 1183
BsaJI	CCNNGG	3 cut(s)	81, 1606, 1649
BsaWI	WCCGGW	1 cut(s)	921
BsaXI	ACNNNNNCTCC	2 cut(s)	1630, 1660
Bsc4I	CCNNNNNNNGG	5 cut(s)	110, 171, 462, 765, 1697
Bse118I	RCCGGY	1 cut(s)	280
Bse1I	ACTGG	4 cut(s)	478, 665, 770, 1595
Bse3DI	GCAATG	2 cut(s)	429, 1095
Bse8I	GATNNNNATC	1 cut(s)	1475
BseAI	TCCGGA	1 cut(s)	921
BseBI	CCWGG	3 cut(s)	397, 494, 1650
BseCI	ATCGAT	1 cut(s)	123
BseDI	CCNNGG	3 cut(s)	81, 1606, 1649
BseGI	GGATG	4 cut(s)	392, 504, 669, 1687
BseJI	GATNNNNATC	1 cut(s)	1475
BseLI	CCNNNNNNNGG	5 cut(s)	110, 171, 462, 765, 1697
BseMI	GCAATG	2 cut(s)	429, 1095
BseMII	CTCAG	8 cut(s)	234, 699, 699, 1194, 1418, 1505, 1742, 1824
BseNI	ACTGG	4 cut(s)	478, 665, 770, 1595
BseSI	GKGCMC	1 cut(s)	639
BseXI	GCAGC	2 cut(s)	105, 1033
Bsh1236I	CGCG	1 cut(s)	540
BshFI	GGCC	8 cut(s)	80, 132, 248, 492, 668, 1654, 1683, 1802
BshVI	ATCGAT	1 cut(s)	123
BsiHKAI	GWGCWC	3 cut(s)	639, 729, 1648
BsiHKCI	CYCGRG	1 cut(s)	55
BsiSI	CCGG	4 cut(s)	281, 535, 922, 1730
BslFI	GGGAC	1 cut(s)	953
BslI	CCNNNNNNNGG	5 cut(s)	110, 171, 462, 765, 1697
BsmAI	GTCTC	5 cut(s)	276, 527, 536, 1183, 1833
BsmBI	CGTCTC	2 cut(s)	527, 536
BsmFI	GGGAC	1 cut(s)	953
BsmI	GAATGC	1 cut(s)	1834
BsnI	GGCC	8 cut(s)	80, 132, 248, 492, 668, 1654, 1683, 1802
Bso31I	GGTCTC	2 cut(s)	276, 1183
BsoBI	CYCGRG	1 cut(s)	55
Bsp119I	TTCGAA	1 cut(s)	46
Bsp1286I	GDGCHC	3 cut(s)	639, 729, 1648
Bsp13I	TCCGGA	1 cut(s)	921
Bsp143I	GATC	6 cut(s)	376, 594, 1088, 1325, 1443, 1846
Bsp1720I	GCTNAGC	1 cut(s)	1810
Bsp19I	CCATGG	1 cut(s)	1606
BspACI	CCGC	4 cut(s)	105, 644, 1414, 1814
BspANI	GGCC	8 cut(s)	80, 132, 248, 492, 668, 1654, 1683, 1802
BspCNI	CTCAG	8 cut(s)	233, 698, 700, 1193, 1417, 1504, 1743, 1823
BspDI	ATCGAT	1 cut(s)	123
BspEI	TCCGGA	1 cut(s)	921
BspFNI	CGCG	1 cut(s)	540
BspHI	TCATGA	2 cut(s)	933, 1588
BspLI	GGNNCC	1 cut(s)	115
BspMAI	CTGCAG	1 cut(s)	1333
BspPI	GGATC	4 cut(s)	602, 1083, 1333, 1438
BspQI	GCTCTTC	2 cut(s)	473, 1152
BspT104I	TTCGAA	1 cut(s)	46
BspTNI	GGTCTC	2 cut(s)	276, 1183
BsrDI	GCAATG	2 cut(s)	429, 1095
BsrFI	RCCGGY	1 cut(s)	280
BsrI	ACTGG	4 cut(s)	478, 665, 770, 1595
BssAI	RCCGGY	1 cut(s)	280
BssECI	CCNNGG	3 cut(s)	81, 1606, 1649
BssMI	GATC	6 cut(s)	376, 594, 1088, 1325, 1443, 1846
BssNI	GRCGYC	3 cut(s)	23, 230, 529
BssT1I	CCWWGG	1 cut(s)	1606
Bst2UI	CCWGG	3 cut(s)	397, 494, 1650
Bst6I	CTCTTC	2 cut(s)	473, 1152
BstACI	GRCGYC	3 cut(s)	23, 230, 529
BstBI	TTCGAA	1 cut(s)	46
BstC8I	GCNNGC	4 cut(s)	105, 810, 970, 1011
BstDEI	CTNAG	8 cut(s)	220, 685, 708, 1180, 1404, 1491, 1751, 1810
BstDSI	CCRYGG	1 cut(s)	1606
BstENI	CCTNNNNNAGG	1 cut(s)	169
BstF5I	GGATG	4 cut(s)	392, 504, 669, 1687
BstFNI	CGCG	1 cut(s)	540
BstH2I	RGCGCY	1 cut(s)	55
BstHHI	GCGC	2 cut(s)	54, 540
BstKTI	GATC	6 cut(s)	379, 597, 1091, 1328, 1446, 1849
BstMAI	GTCTC	5 cut(s)	276, 527, 536, 1183, 1833
BstMBI	GATC	6 cut(s)	376, 594, 1088, 1325, 1443, 1846
BstMWI	GCNNNNNNNGC	5 cut(s)	40, 49, 60, 308, 535
BstNI	CCWGG	3 cut(s)	397, 494, 1650
BstNSI	RCATGY	1 cut(s)	1021
BstSCI	CCNGG	3 cut(s)	395, 492, 1648
BstSFI	CTRYAG	2 cut(s)	1064, 1329
BstSLI	GKGCMC	1 cut(s)	639
BstUI	CGCG	1 cut(s)	540
BstV1I	GCAGC	2 cut(s)	105, 1033
BstV2I	GAAGAC	1 cut(s)	1467
BstX2I	RGATCY	2 cut(s)	1088, 1325
BstYI	RGATCY	2 cut(s)	1088, 1325
Bsu15I	ATCGAT	1 cut(s)	123
BsuRI	GGCC	8 cut(s)	80, 132, 248, 492, 668, 1654, 1683, 1802
BsuTUI	ATCGAT	1 cut(s)	123
BtgI	CCRYGG	1 cut(s)	1606
BtrI	CACGTC	1 cut(s)	1279
BtsCI	GGATG	4 cut(s)	392, 504, 669, 1687
BtsIMutI	CAGTG	5 cut(s)	471, 550, 919, 1389, 1602
Cac8I	GCNNGC	4 cut(s)	105, 810, 970, 1011
CciI	TCATGA	2 cut(s)	933, 1588
CfoI	GCGC	2 cut(s)	54, 540
Cfr10I	RCCGGY	1 cut(s)	280
Cfr13I	GGNCC	3 cut(s)	712, 1069, 1652
ClaI	ATCGAT	1 cut(s)	123
CseI	GACGC	5 cut(s)	12, 219, 518, 529, 1472
Csp6I	GTAC	4 cut(s)	217, 371, 443, 1578
CviQI	GTAC	4 cut(s)	217, 371, 443, 1578
DdeI	CTNAG	8 cut(s)	220, 685, 708, 1180, 1404, 1491, 1751, 1810
DpnI	GATC	6 cut(s)	378, 596, 1090, 1327, 1445, 1848
DpnII	GATC	6 cut(s)	376, 594, 1088, 1325, 1443, 1846
DraI	TTTAAA	1 cut(s)	556
EaeI	YGGCCR	4 cut(s)	130, 666, 1681, 1800
Eam1104I	CTCTTC	2 cut(s)	473, 1152
EarI	CTCTTC	2 cut(s)	473, 1152
Eco130I	CCWWGG	1 cut(s)	1606
Eco31I	GGTCTC	2 cut(s)	276, 1183
Eco32I	GATATC	1 cut(s)	179
Eco47I	GGWCC	2 cut(s)	712, 1069
Eco57I	CTGAAG	1 cut(s)	1176
Eco88I	CYCGRG	1 cut(s)	55
EcoNI	CCTNNNNNAGG	1 cut(s)	169
EcoO109I	RGGNCCY	2 cut(s)	1069, 1652
EcoRII	CCWGG	3 cut(s)	395, 492, 1648
EcoRV	GATATC	1 cut(s)	179
EcoT14I	CCWWGG	1 cut(s)	1606
ErhI	CCWWGG	1 cut(s)	1606
Esp3I	CGTCTC	2 cut(s)	527, 536
FalI	AAGNNNNNCTT	2 cut(s)	1701, 1733
FaqI	GGGAC	1 cut(s)	953
FauI	CCCGC	2 cut(s)	112, 637
FauNDI	CATATG	2 cut(s)	614, 754
FbaI	TGATCA	2 cut(s)	376, 1846
Fnu4HI	GCNGC	2 cut(s)	94, 1022
FokI	GGATG	4 cut(s)	379, 491, 676, 1674
Fsp4HI	GCNGC	2 cut(s)	94, 1022
FspBI	CTAG	4 cut(s)	368, 563, 1259, 1322
GlaI	GCGC	2 cut(s)	53, 539
GluI	GCNGC	2 cut(s)	94, 1022
GsuI	CTGGAG	2 cut(s)	1287, 1632
HaeII	RGCGCY	1 cut(s)	55
HaeIII	GGCC	8 cut(s)	80, 132, 248, 492, 668, 1654, 1683, 1802
HapII	CCGG	4 cut(s)	281, 535, 922, 1730
HgaI	GACGC	5 cut(s)	12, 219, 518, 529, 1472
HhaI	GCGC	2 cut(s)	54, 540
Hin1I	GRCGYC	3 cut(s)	23, 230, 529
Hin6I	GCGC	2 cut(s)	52, 538
HinP1I	GCGC	2 cut(s)	52, 538
HincII	GTYRAC	2 cut(s)	357, 817
HindII	GTYRAC	2 cut(s)	357, 817
HinfI	GANTC	6 cut(s)	867, 941, 1149, 1668, 1703, 1733
HpaI	GTTAAC	1 cut(s)	817
HpaII	CCGG	4 cut(s)	281, 535, 922, 1730
HphI	GGTGA	3 cut(s)	769, 812, 1651
Hpy166II	GTNNAC	7 cut(s)	217, 357, 548, 637, 817, 910, 1580
Hpy188I	TCNGA	7 cut(s)	599, 688, 962, 1077, 1156, 1183, 1752
Hpy188III	TCNNGA	5 cut(s)	922, 934, 986, 1033, 1589
Hpy8I	GTNNAC	7 cut(s)	217, 357, 548, 637, 817, 910, 1580
Hpy99I	CGWCG	3 cut(s)	10, 28, 235
HpyCH4IV	ACGT	4 cut(s)	194, 429, 603, 1278
HpyCH4V	TGCA	8 cut(s)	139, 392, 637, 808, 968, 1021, 1228, 1331
HpyF10VI	GCNNNNNNNGC	5 cut(s)	40, 49, 60, 308, 535
HpyF3I	CTNAG	8 cut(s)	220, 685, 708, 1180, 1404, 1491, 1751, 1810
HpySE526I	ACGT	4 cut(s)	194, 429, 603, 1278
Hsp92I	GRCGYC	3 cut(s)	23, 230, 529
HspAI	GCGC	2 cut(s)	52, 538
Kpn2I	TCCGGA	1 cut(s)	921
Ksp22I	TGATCA	2 cut(s)	376, 1846
KspAI	GTTAAC	1 cut(s)	817
Kzo9I	GATC	6 cut(s)	376, 594, 1088, 1325, 1443, 1846
LguI	GCTCTTC	2 cut(s)	473, 1152
LmnI	GCTCC	3 cut(s)	166, 1268, 1651
Lsp1109I	GCAGC	2 cut(s)	105, 1033
LweI	GCATC	5 cut(s)	289, 401, 513, 744, 1425
MaeI	CTAG	4 cut(s)	368, 563, 1259, 1322
MaeII	ACGT	4 cut(s)	194, 429, 603, 1278
MaeIII	GTNAC	8 cut(s)	403, 584, 619, 746, 775, 800, 1044, 1521
MalI	GATC	6 cut(s)	378, 596, 1090, 1327, 1445, 1848
MboI	GATC	6 cut(s)	376, 594, 1088, 1325, 1443, 1846
MboII	GAAGA	7 cut(s)	298, 460, 1067, 1169, 1472, 1568, 1832
MfeI	CAATTG	2 cut(s)	1172, 1791
MflI	RGATCY	2 cut(s)	1088, 1325
MhlI	GDGCHC	3 cut(s)	639, 729, 1648
MlsI	TGGCCA	2 cut(s)	668, 1683
MluNI	TGGCCA	2 cut(s)	668, 1683
MlyI	GAGTC	4 cut(s)	861, 935, 1677, 1697
MmeI	TCCRAC	4 cut(s)	865, 1265, 1640, 1752
Mox20I	TGGCCA	2 cut(s)	668, 1683
MroI	TCCGGA	1 cut(s)	921
MroXI	GAANNNNTTC	3 cut(s)	833, 1059, 1200
MscI	TGGCCA	2 cut(s)	668, 1683
MseI	TTAA	3 cut(s)	555, 816, 1508
MslI	CAYNNNNRTG	2 cut(s)	1587, 1692
Msp20I	TGGCCA	2 cut(s)	668, 1683
MspA1I	CMGCKG	1 cut(s)	1814
MspI	CCGG	4 cut(s)	281, 535, 922, 1730
MspR9I	CCNGG	3 cut(s)	397, 494, 1650
MunI	CAATTG	2 cut(s)	1172, 1791
Mva1269I	GAATGC	1 cut(s)	1834
MvaI	CCWGG	3 cut(s)	397, 494, 1650
MvnI	CGCG	1 cut(s)	540
MwoI	GCNNNNNNNGC	5 cut(s)	40, 49, 60, 308, 535
NcoI	CCATGG	1 cut(s)	1606
NdeI	CATATG	2 cut(s)	614, 754
NdeII	GATC	6 cut(s)	376, 594, 1088, 1325, 1443, 1846
NlaIV	GGNNCC	1 cut(s)	115
NmeAIII	GCCGAG	1 cut(s)	620
NmuCI	GTSAC	2 cut(s)	775, 800
NspI	RCATGY	1 cut(s)	1021
NspV	TTCGAA	1 cut(s)	46
PaeR7I	CTCGAG	1 cut(s)	55
PagI	TCATGA	2 cut(s)	933, 1588
PciSI	GCTCTTC	2 cut(s)	473, 1152
PcsI	WCGNNNNNNNCGW	2 cut(s)	426, 526
PctI	GAATGC	1 cut(s)	1834
PdmI	GAANNNNTTC	3 cut(s)	833, 1059, 1200
PfeI	GAWTC	2 cut(s)	1149, 1733
PflMI	CCANNNNNTGG	1 cut(s)	765
PkrI	GCNGC	2 cut(s)	95, 1023
PleI	GAGTC	4 cut(s)	861, 935, 1676, 1697
PpsI	GAGTC	4 cut(s)	861, 935, 1676, 1697
PpuMI	RGGWCCY	1 cut(s)	1069
Psp5II	RGGWCCY	1 cut(s)	1069
Psp6I	CCWGG	3 cut(s)	395, 492, 1648
PspGI	CCWGG	3 cut(s)	395, 492, 1648
PspN4I	GGNNCC	1 cut(s)	115
PspPI	GGNCC	3 cut(s)	712, 1069, 1652
PspPPI	RGGWCCY	1 cut(s)	1069
PspXI	VCTCGAGB	1 cut(s)	55
PstI	CTGCAG	1 cut(s)	1333
PsuI	RGATCY	2 cut(s)	1088, 1325
RsaI	GTAC	4 cut(s)	218, 372, 444, 1579
RsaNI	GTAC	4 cut(s)	217, 371, 443, 1578
RseI	CAYNNNNRTG	2 cut(s)	1587, 1692
SapI	GCTCTTC	2 cut(s)	473, 1152
SaqAI	TTAA	3 cut(s)	555, 816, 1508
SatI	GCNGC	2 cut(s)	94, 1022
Sau3AI	GATC	6 cut(s)	376, 594, 1088, 1325, 1443, 1846
Sau96I	GGNCC	3 cut(s)	712, 1069, 1652
ScaI	AGTACT	1 cut(s)	372
SchI	GAGTC	4 cut(s)	861, 935, 1677, 1697
ScrFI	CCNGG	3 cut(s)	397, 494, 1650
SduI	GDGCHC	3 cut(s)	639, 729, 1648
SfaNI	GCATC	5 cut(s)	289, 401, 513, 744, 1425
SfcI	CTRYAG	2 cut(s)	1064, 1329
Sfr274I	CTCGAG	1 cut(s)	55
SfuI	TTCGAA	1 cut(s)	46
SinI	GGWCC	2 cut(s)	712, 1069
SlaI	CTCGAG	1 cut(s)	55
SmiMI	CAYNNNNRTG	2 cut(s)	1587, 1692
SmlI	CTYRAG	2 cut(s)	55, 1025
SmoI	CTYRAG	2 cut(s)	55, 1025
SsiI	CCGC	4 cut(s)	105, 644, 1414, 1814
SspI	AATATT	1 cut(s)	1199
SspMI	CTAG	4 cut(s)	368, 563, 1259, 1322
StyD4I	CCNGG	3 cut(s)	395, 492, 1648
StyI	CCWWGG	1 cut(s)	1606
TaiI	ACGT	4 cut(s)	197, 432, 606, 1281
TaqI	TCGA	8 cut(s)	46, 56, 89, 123, 240, 297, 1215, 1706
TatI	WGTACW	3 cut(s)	216, 370, 1577
TfiI	GAWTC	2 cut(s)	1149, 1733
Tru1I	TTAA	3 cut(s)	555, 816, 1508
Tru9I	TTAA	3 cut(s)	555, 816, 1508
TscAI	CASTG	5 cut(s)	478, 550, 919, 1389, 1602
TseFI	GTSAC	2 cut(s)	775, 800
TseI	GCWGC	2 cut(s)	93, 1021
Tsp45I	GTSAC	2 cut(s)	775, 800
TspDTI	ATGAA	8 cut(s)	4, 922, 1202, 1473, 1605, 1667, 1710, 1712
TspGWI	ACGGA	2 cut(s)	1103, 1316
TspRI	CASTG	5 cut(s)	478, 550, 919, 1389, 1602
Van91I	CCANNNNNTGG	1 cut(s)	765
VneI	GTGCAC	1 cut(s)	635
VpaK11BI	GGWCC	2 cut(s)	712, 1069
XagI	CCTNNNNNAGG	1 cut(s)	169
XapI	RAATTY	4 cut(s)	73, 149, 1236, 1530
XceI	RCATGY	1 cut(s)	1021
XhoI	CTCGAG	1 cut(s)	55
XmnI	GAANNNNTTC	3 cut(s)	833, 1059, 1200
XspI	CTAG	4 cut(s)	368, 563, 1259, 1322
ZrmI	AGTACT	1 cut(s)	372

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.