Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009077

Physical Location & Seq

Genomic Coordinates Forward (+)

12804180 .. 12811702

Gene Structure 7523 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1134700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1830 bp

ATGTCGTCGTGGTCGGCAACTGCATCGCCGCTGCCTTTGCTGTTCCGGAGGCGTTCGGGCGGGGAGATTAGGAATTTGGCCTCTGTGTCAAGCAGCATCTTACCGGCTTTTGGAACCATTGTCGATGAAGCCCACTTGCAGCTCAAGAAATTTGTTATTGCTCCTTATGACAGGAGATATAGGTGGTGGCAGGCGTTTTTGGTGGTGCTGGTGATATACTCAGCATGGGCGTCGCCGTTCGAACTGGCCTTCAAAAAGGTGGCAACTGGATCACTCCTGCCCGTAGATTTGGCTGTCGATGCATTCTTCGCCGTTGATATCGTCCTAACTTTCTTCGTGGCGTACTTGGACAAGACAACCTACCTCTTAGTACTTGATCACAAGAAAATCGCTTTGAGGTATGTTACAAGAATATATTTTCCATTTGACGTAGCATCTACTCTACCATTTCATGTGATATATAGAATCCTTACCGGCAATATGCACCATCAACAAGTCTTTCGCTTCATCAATATGCTTCGACTATGGCGTCTCAGGCGTGTTAGTGAATTCTTCAAAAGGCTAGAGAAAGACACACGCTTCAGCTACTTTTGGGTTCGATACATCAAACTAATTTGTGTTACACTATTCGTAGTGCACTCATCAGGTTGCTTCTTCTTCTGGATGGCAACCCGTCAGAAAGAAGCAGCGGACACATGGATCGGGATCCAAATCAACGACTTCGAGACCAGAAGCATCTGGTTGTGTTACACGTATTCCATCTACTGGGCCACCGTCACTCTCACCACCGTTGGATATGGCGACATACATGCGGTCAACTTCAAGGAGAAGATTTTTACCATCTTCTATATGCTTTTCAACATTGGCTTCGTTGCTTACATAATTGGAAACATGACCAATCTCATCGTCCATAGCGCTGTCCGAACTTTATTCATGAGGAATGCAATCAATGAGATACTGAGATATGCAAGTAAGAACAGACTTCCTCAAGGGTTGAAGGAGCAAATGCTGGCTCATATGCAGCTCAAGTTCAAGACTGCAGAGTTGCAGCAAGAAGTGGTGCTTCAAGACCTTCCCAAGGCAATCAGATCCAGCATTGCCCACCATCTTTTCCAAAAAACCGTTGAGAAAACCTACCTGTTCAAAGGAGTCTCTGAACACCTTACTGTCCAATTGATTTCAGAGATGAAAGCAGAATATTTTCCACCTAAAGTTGACATCATCTTACAAAACGAAATCCCAACAGATTTCTACATTCTAGTAACTGGAGCATTGTTTTTGTCAAACCTAGGATCTGCAGATTTGGTAGGAGAAATTGGGATATGTTTCAACATCCCCCAACCTTTTACAGTGAGAACAAAAAAGCTTTCTCAGGTTATTCGGATCAGTCATCAATCATTCAAGCAAATGGTACAGTCACAGAATGAAGACAGCAAGATAATATTCAAAAACTTAATCGAGCACTTGAGAGACTTGCAAACAGAGGTGCGAGAAGAACTACCGTTTCTAACAGAATTGTTGGATGGCCTAAATATAGAGCATATACAACCAAACGAACCAGTGAATTACCAAGGAGAAGCGAACATGGAAGAAACAAGCGAAACTTCCCCTCCGGGGCCAATTACTATGAGAGTCATAATTCACGGACATCATCCAGATGAAGGTGACTCGAATGGAAAACTCATTCATTTGCCCGAGTCAGTGGAAGACCTTCTGAAATTAGCAGAGAAAAAGTTGGGAAAAAGAGGCAGCACGGTTGTGATGGCGGACGGCTCACAGGTCGAAGAGCTAAATGCATTAAGAGACAACGACCGCTTGTTTATCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009605 GO:0009607 GO:0009624 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ion_trans KHA cNMP_binding

Protein Analysis

610

Amino Acids

70.32

Weight (kDa)

8.72

Isoelectric Point (pI)

43.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	60 - 305	8.1e-37	Ion transport protein
Ion_trans_2	PF07885	247 - 302	1.2e-12	Ion channel
cNMP_binding	PF00027	400 - 473	2.1e-06	Cyclic nucleotide-binding domain
KHA	PF11834	544 - 608	1.3e-21	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009077)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32650 AT4G32650 AT4G32650
fragaria_vesca	FvH4_2g30280 FvH4_2g30280
malus_domestica	MD08G1134700.v1.1 MD15G1113600.v1.1
prunus_persica	Prupe.1G464600_v2.0.a1 Prupe.1G464600_v2.0.a1
pyrus_communis	pycom08g11370
rosa_chinensis	RchiOBHm_Chr6g0312591
rosa_laevigata	RLG00000010313
rosa_multiflora	Rmu_co8350831.1_g000001 Rmu_sc0003094.1_g000003 Rmu_sc0008363.1_g000002
rosa_roxburghii	Rroxscaffold_7G00156670
rosa_rugosa	Rorug06G0396700
rosa_samantha	Rh6AG514600 Rh6BG525400 Rh6CG530600 Rh6DG516700
rosa_wichuraiana	Rw6G044710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	528
AccB7I	CCANNNNNTGG	1 cut(s)	765
AccIII	TCCGGA	1 cut(s)	45
AciI	CCGC	6 cut(s)	29, 60, 689, 812, 1766, 1813
AclWI	GGATC	7 cut(s)	277, 700, 707, 713, 1083, 1300, 1391
AcsI	RAATTY	3 cut(s)	73, 149, 548
AcuI	CTGAAG	1 cut(s)	565
AcyI	GRCGYC	2 cut(s)	230, 529
AfaI	GTAC	3 cut(s)	344, 372, 1413
AfeI	AGCGCT	1 cut(s)	916
AfiI	CCNNNNNNNGG	5 cut(s)	110, 765, 1078, 1614, 1661
AflIII	ACRYGT	1 cut(s)	750
AleI	CACNNNNGTG	1 cut(s)	1757
AluBI	AGCT	5 cut(s)	142, 585, 1024, 1366, 1789
AluI	AGCT	5 cut(s)	142, 585, 1024, 1366, 1789
Alw21I	GWGCWC	2 cut(s)	639, 1464
Alw26I	GTCTC	5 cut(s)	536, 719, 1156, 1464, 1797
Alw44I	GTGCAC	1 cut(s)	635
AlwI	GGATC	7 cut(s)	277, 700, 707, 713, 1083, 1300, 1391
Ama87I	CYCGRG	1 cut(s)	1694
Aor13HI	TCCGGA	1 cut(s)	45
Aor51HI	AGCGCT	1 cut(s)	916
AoxI	GGCC	5 cut(s)	78, 246, 768, 1525, 1616
ApaLI	GTGCAC	1 cut(s)	635
ApeKI	GCWGC	7 cut(s)	31, 93, 139, 686, 1021, 1048, 1749
ApoI	RAATTY	3 cut(s)	73, 149, 548
Asp700I	GAANNNNTTC	2 cut(s)	1200, 1710
AspA2I	CCTAGG	1 cut(s)	1288
AspLEI	GCGC	1 cut(s)	917
AspS9I	GGNCC	2 cut(s)	768, 1616
AsuC2I	CCSGG	1 cut(s)	1614
AsuHPI	GGTGA	3 cut(s)	223, 775, 1676
AsuII	TTCGAA	1 cut(s)	240
AvaI	CYCGRG	1 cut(s)	1694
AvrII	CCTAGG	1 cut(s)	1288
BaeGI	GKGCMC	1 cut(s)	639
BamHI	GGATCC	1 cut(s)	705
BbsI	GAAGAC	2 cut(s)	1434, 1713
Bbv12I	GWGCWC	2 cut(s)	639, 1464
BbvI	GCAGC	7 cut(s)	18, 105, 151, 698, 1033, 1060, 1761
BccI	CCATC	7 cut(s)	495, 658, 767, 848, 1113, 1517, 1756
BceAI	ACGGC	3 cut(s)	220, 296, 1786
BcgI	CGANNNNNNTGC	6 cut(s)	6, 40, 213, 247, 791, 825
BclI	TGATCA	1 cut(s)	376
BcnI	CCSGG	1 cut(s)	1614
BcoDI	GTCTC	5 cut(s)	536, 719, 1156, 1464, 1797
BfaI	CTAG	3 cut(s)	563, 1259, 1289
BfmI	CTRYAG	2 cut(s)	1038, 1296
BfoI	RGCGCY	1 cut(s)	918
BisI	GCNGC	8 cut(s)	29, 32, 94, 140, 687, 1022, 1049, 1750
BlnI	CCTAGG	1 cut(s)	1288
BlsI	GCNGC	8 cut(s)	30, 33, 95, 141, 688, 1023, 1050, 1751
BmcAI	AGTACT	1 cut(s)	372
Bme1390I	CCNGG	1 cut(s)	1614
BmeT110I	CYCGRG	1 cut(s)	1694
BmgT120I	GGNCC	2 cut(s)	768, 1616
BmiI	GGNNCC	3 cut(s)	115, 707, 1617
BmrFI	CCNGG	1 cut(s)	1614
BmrI	ACTGGG	1 cut(s)	775
BmsI	GCATC	5 cut(s)	32, 105, 289, 443, 744
BmuI	ACTGGG	1 cut(s)	775
BpiI	GAAGAC	2 cut(s)	1434, 1713
BpmI	CTGGAG	1 cut(s)	1287
Bpu14I	TTCGAA	1 cut(s)	240
BpuEI	CTTGAG	4 cut(s)	128, 972, 1010, 1486
BpuMI	CCSGG	1 cut(s)	1614
BsaAI	YACGTR	1 cut(s)	753
BsaBI	GATNNNNATC	2 cut(s)	704, 710
BsaHI	GRCGYC	2 cut(s)	230, 529
BsaI	GGTCTC	1 cut(s)	719
BsaJI	CCNNGG	4 cut(s)	1077, 1288, 1570, 1613
BsaWI	WCCGGW	1 cut(s)	45
BsaXI	ACNNNNNCTCC	2 cut(s)	1594, 1624
Bsc4I	CCNNNNNNNGG	5 cut(s)	110, 765, 1078, 1614, 1661
Bse118I	RCCGGY	2 cut(s)	103, 473
Bse1I	ACTGG	5 cut(s)	249, 271, 770, 1270, 1559
Bse3DI	GCAATG	1 cut(s)	1095
Bse8I	GATNNNNATC	2 cut(s)	704, 710
BseAI	TCCGGA	1 cut(s)	45
BseDI	CCNNGG	4 cut(s)	1077, 1288, 1570, 1613
BseGI	GGATG	4 cut(s)	669, 1332, 1528, 1651
BseJI	GATNNNNATC	2 cut(s)	704, 710
BseLI	CCNNNNNNNGG	5 cut(s)	110, 765, 1078, 1614, 1661
BseMI	GCAATG	1 cut(s)	1095
BseMII	CTCAG	4 cut(s)	234, 547, 950, 1385
BseNI	ACTGG	5 cut(s)	249, 271, 770, 1270, 1559
BseSI	GKGCMC	1 cut(s)	639
BseXI	GCAGC	7 cut(s)	18, 105, 151, 698, 1033, 1060, 1761
Bsh1285I	CGRYCG	1 cut(s)	1813
BshFI	GGCC	5 cut(s)	80, 248, 770, 1527, 1618
BsiEI	CGRYCG	1 cut(s)	1813
BsiHKAI	GWGCWC	2 cut(s)	639, 1464
BsiHKCI	CYCGRG	1 cut(s)	1694
BsiSI	CCGG	4 cut(s)	46, 104, 474, 1613
BslI	CCNNNNNNNGG	5 cut(s)	110, 765, 1078, 1614, 1661
BsmAI	GTCTC	5 cut(s)	536, 719, 1156, 1464, 1797
BsmBI	CGTCTC	1 cut(s)	536
BsmI	GAATGC	2 cut(s)	302, 946
BsnI	GGCC	5 cut(s)	80, 248, 770, 1527, 1618
Bso31I	GGTCTC	1 cut(s)	719
BsoBI	CYCGRG	1 cut(s)	1694
Bsp119I	TTCGAA	1 cut(s)	240
Bsp1286I	GDGCHC	2 cut(s)	639, 1464
Bsp13I	TCCGGA	1 cut(s)	45
Bsp143I	GATC	7 cut(s)	269, 376, 699, 705, 1088, 1292, 1383
BspACI	CCGC	6 cut(s)	29, 60, 689, 812, 1766, 1813
BspANI	GGCC	5 cut(s)	80, 248, 770, 1527, 1618
BspCNI	CTCAG	4 cut(s)	233, 546, 951, 1384
BspEI	TCCGGA	1 cut(s)	45
BspHI	TCATGA	1 cut(s)	933
BspLI	GGNNCC	3 cut(s)	115, 707, 1617
BspMAI	CTGCAG	2 cut(s)	1042, 1300
BspPI	GGATC	7 cut(s)	277, 700, 707, 713, 1083, 1300, 1391
BspQI	GCTCTTC	1 cut(s)	1779
BspT104I	TTCGAA	1 cut(s)	240
BspTNI	GGTCTC	1 cut(s)	719
BsrDI	GCAATG	1 cut(s)	1095
BsrFI	RCCGGY	2 cut(s)	103, 473
BsrI	ACTGG	5 cut(s)	249, 271, 770, 1270, 1559
BssAI	RCCGGY	2 cut(s)	103, 473
BssECI	CCNNGG	4 cut(s)	1077, 1288, 1570, 1613
BssMI	GATC	7 cut(s)	269, 376, 699, 705, 1088, 1292, 1383
BssNI	GRCGYC	2 cut(s)	230, 529
BssT1I	CCWWGG	3 cut(s)	1077, 1288, 1570
Bst4CI	ACNGT	8 cut(s)	775, 790, 1123, 1168, 1351, 1416, 1503, 1756
Bst6I	CTCTTC	1 cut(s)	1779
BstACI	GRCGYC	2 cut(s)	230, 529
BstBAI	YACGTR	1 cut(s)	753
BstBI	TTCGAA	1 cut(s)	240
BstC8I	GCNNGC	2 cut(s)	192, 1011
BstDEI	CTNAG	5 cut(s)	220, 367, 533, 959, 1371
BstENI	CCTNNNNNAGG	1 cut(s)	1076
BstF5I	GGATG	4 cut(s)	669, 1332, 1528, 1651
BstH2I	RGCGCY	1 cut(s)	918
BstHHI	GCGC	1 cut(s)	917
BstKTI	GATC	7 cut(s)	272, 379, 702, 708, 1091, 1295, 1386
BstMAI	GTCTC	5 cut(s)	536, 719, 1156, 1464, 1797
BstMBI	GATC	7 cut(s)	269, 376, 699, 705, 1088, 1292, 1383
BstMCI	CGRYCG	1 cut(s)	1813
BstMWI	GCNNNNNNNGC	4 cut(s)	37, 299, 308, 535
BstNSI	RCATGY	1 cut(s)	812
BstSCI	CCNGG	1 cut(s)	1612
BstSFI	CTRYAG	2 cut(s)	1038, 1296
BstSLI	GKGCMC	1 cut(s)	639
BstV1I	GCAGC	7 cut(s)	18, 105, 151, 698, 1033, 1060, 1761
BstV2I	GAAGAC	2 cut(s)	1434, 1713
BstX2I	RGATCY	3 cut(s)	705, 1088, 1292
BstYI	RGATCY	3 cut(s)	705, 1088, 1292
BsuRI	GGCC	5 cut(s)	80, 248, 770, 1527, 1618
BtgZI	GCGATG	1 cut(s)	9
BtsCI	GGATG	4 cut(s)	669, 1332, 1528, 1651
BtsIMutI	CAGTG	3 cut(s)	1356, 1566, 1707
Cac8I	GCNNGC	2 cut(s)	192, 1011
CciI	TCATGA	1 cut(s)	933
CfoI	GCGC	1 cut(s)	917
Cfr10I	RCCGGY	2 cut(s)	103, 473
Cfr13I	GGNCC	2 cut(s)	768, 1616
CseI	GACGC	2 cut(s)	219, 518
Csp6I	GTAC	3 cut(s)	343, 371, 1412
CviAII	CATG	7 cut(s)	225, 452, 696, 809, 892, 934, 1585
CviQI	GTAC	3 cut(s)	343, 371, 1412
DdeI	CTNAG	5 cut(s)	220, 367, 533, 959, 1371
DpnI	GATC	7 cut(s)	271, 378, 701, 707, 1090, 1294, 1385
DpnII	GATC	7 cut(s)	269, 376, 699, 705, 1088, 1292, 1383
DrdI	GACNNNNNNGTC	1 cut(s)	528
DseDI	GACNNNNNNGTC	1 cut(s)	528
Eam1104I	CTCTTC	1 cut(s)	1779
EarI	CTCTTC	1 cut(s)	1779
EciI	GGCGGA	1 cut(s)	1781
Eco130I	CCWWGG	3 cut(s)	1077, 1288, 1570
Eco31I	GGTCTC	1 cut(s)	719
Eco32I	GATATC	1 cut(s)	319
Eco47III	AGCGCT	1 cut(s)	916
Eco57I	CTGAAG	1 cut(s)	565
Eco88I	CYCGRG	1 cut(s)	1694
EcoNI	CCTNNNNNAGG	1 cut(s)	1076
EcoRI	GAATTC	1 cut(s)	548
EcoRV	GATATC	1 cut(s)	319
EcoT14I	CCWWGG	3 cut(s)	1077, 1288, 1570
EcoT22I	ATGCAT	2 cut(s)	304, 1798
ErhI	CCWWGG	3 cut(s)	1077, 1288, 1570
Esp3I	CGTCTC	1 cut(s)	536
FaeI	CATG	7 cut(s)	228, 455, 699, 812, 895, 937, 1588
FalI	AAGNNNNNCTT	4 cut(s)	1047, 1079, 1588, 1620
FatI	CATG	7 cut(s)	224, 451, 695, 808, 891, 933, 1584
FauI	CCCGC	1 cut(s)	53
FauNDI	CATATG	1 cut(s)	1017
FbaI	TGATCA	1 cut(s)	376
Fnu4HI	GCNGC	8 cut(s)	29, 32, 94, 140, 687, 1022, 1049, 1750
FokI	GGATG	4 cut(s)	676, 1319, 1535, 1638
Fsp4HI	GCNGC	8 cut(s)	29, 32, 94, 140, 687, 1022, 1049, 1750
FspBI	CTAG	3 cut(s)	563, 1259, 1289
GlaI	GCGC	1 cut(s)	916
GluI	GCNGC	8 cut(s)	29, 32, 94, 140, 687, 1022, 1049, 1750
GsuI	CTGGAG	1 cut(s)	1287
HaeII	RGCGCY	1 cut(s)	918
HaeIII	GGCC	5 cut(s)	80, 248, 770, 1527, 1618
HapII	CCGG	4 cut(s)	46, 104, 474, 1613
HgaI	GACGC	2 cut(s)	219, 518
HhaI	GCGC	1 cut(s)	917
Hin1I	GRCGYC	2 cut(s)	230, 529
Hin1II	CATG	7 cut(s)	228, 455, 699, 812, 895, 937, 1588
Hin6I	GCGC	1 cut(s)	915
HinP1I	GCGC	1 cut(s)	915
HincII	GTYRAC	2 cut(s)	817, 1216
HindII	GTYRAC	2 cut(s)	817, 1216
HindIII	AAGCTT	1 cut(s)	1364
HinfI	GANTC	5 cut(s)	465, 1149, 1632, 1667, 1697
HpaII	CCGG	4 cut(s)	46, 104, 474, 1613
HphI	GGTGA	3 cut(s)	223, 775, 1676
Hpy166II	GTNNAC	3 cut(s)	637, 817, 1216
Hpy188I	TCNGA	7 cut(s)	678, 923, 1088, 1156, 1183, 1383, 1716
Hpy188III	TCNNGA	9 cut(s)	46, 145, 661, 703, 724, 934, 1033, 1067, 1655
Hpy8I	GTNNAC	3 cut(s)	637, 817, 1216
Hpy99I	CGWCG	2 cut(s)	10, 235
HpyAV	CCTTC	5 cut(s)	259, 991, 1082, 1655, 1721
HpyCH4III	ACNGT	8 cut(s)	775, 790, 1123, 1168, 1351, 1416, 1503, 1756
HpyCH4IV	ACGT	2 cut(s)	429, 752
HpyF10VI	GCNNNNNNNGC	4 cut(s)	37, 299, 308, 535
HpyF3I	CTNAG	5 cut(s)	220, 367, 533, 959, 1371
HpySE526I	ACGT	2 cut(s)	429, 752
Hsp92I	GRCGYC	2 cut(s)	230, 529
Hsp92II	CATG	7 cut(s)	228, 455, 699, 812, 895, 937, 1588
HspAI	GCGC	1 cut(s)	915
Kpn2I	TCCGGA	1 cut(s)	45
Ksp22I	TGATCA	1 cut(s)	376
Kzo9I	GATC	7 cut(s)	269, 376, 699, 705, 1088, 1292, 1383
LguI	GCTCTTC	1 cut(s)	1779
LmnI	GCTCC	3 cut(s)	166, 1000, 1268
Lsp1109I	GCAGC	7 cut(s)	18, 105, 151, 698, 1033, 1060, 1761
LweI	GCATC	5 cut(s)	32, 105, 289, 443, 744
MaeI	CTAG	3 cut(s)	563, 1259, 1289
MaeII	ACGT	2 cut(s)	429, 752
MaeIII	GTNAC	7 cut(s)	403, 619, 746, 775, 1261, 1416, 1664
MalI	GATC	7 cut(s)	271, 378, 701, 707, 1090, 1294, 1385
MboI	GATC	7 cut(s)	269, 376, 699, 705, 1088, 1292, 1383
MfeI	CAATTG	1 cut(s)	1172
MflI	RGATCY	3 cut(s)	705, 1088, 1292
MhlI	GDGCHC	2 cut(s)	639, 1464
MlyI	GAGTC	4 cut(s)	1158, 1641, 1661, 1706
MmeI	TCCRAC	2 cut(s)	772, 1500
MnlI	CCTC	9 cut(s)	42, 91, 374, 390, 930, 996, 1477, 1620, 1739
Mph1103I	ATGCAT	2 cut(s)	304, 1798
MroI	TCCGGA	1 cut(s)	45
MroXI	GAANNNNTTC	2 cut(s)	1200, 1710
MseI	TTAA	2 cut(s)	1454, 1799
MslI	CAYNNNNRTG	3 cut(s)	512, 1656, 1757
MspA1I	CMGCKG	2 cut(s)	31, 689
MspI	CCGG	4 cut(s)	46, 104, 474, 1613
MspR9I	CCNGG	1 cut(s)	1614
MunI	CAATTG	1 cut(s)	1172
Mva1269I	GAATGC	2 cut(s)	302, 946
MwoI	GCNNNNNNNGC	4 cut(s)	37, 299, 308, 535
NciI	CCSGG	1 cut(s)	1614
NdeI	CATATG	1 cut(s)	1017
NdeII	GATC	7 cut(s)	269, 376, 699, 705, 1088, 1292, 1383
NlaIII	CATG	7 cut(s)	228, 455, 699, 812, 895, 937, 1588
NlaIV	GGNNCC	3 cut(s)	115, 707, 1617
NmuCI	GTSAC	3 cut(s)	775, 1416, 1664
NsiI	ATGCAT	2 cut(s)	304, 1798
NspI	RCATGY	1 cut(s)	812
NspV	TTCGAA	1 cut(s)	240
OliI	CACNNNNGTG	1 cut(s)	1757
PagI	TCATGA	1 cut(s)	933
PciSI	GCTCTTC	1 cut(s)	1779
PcsI	WCGNNNNNNNCGW	1 cut(s)	526
PctI	GAATGC	2 cut(s)	302, 946
PdmI	GAANNNNTTC	2 cut(s)	1200, 1710
PfeI	GAWTC	1 cut(s)	465
PflMI	CCANNNNNTGG	1 cut(s)	765
PkrI	GCNGC	8 cut(s)	30, 33, 95, 141, 688, 1023, 1050, 1751
PleI	GAGTC	4 cut(s)	1157, 1640, 1661, 1705
PpsI	GAGTC	4 cut(s)	1157, 1640, 1661, 1705
Ppu21I	YACGTR	1 cut(s)	753
PspN4I	GGNNCC	3 cut(s)	115, 707, 1617
PspPI	GGNCC	2 cut(s)	768, 1616
PstI	CTGCAG	2 cut(s)	1042, 1300
PsuI	RGATCY	3 cut(s)	705, 1088, 1292
RsaI	GTAC	3 cut(s)	344, 372, 1413
RsaNI	GTAC	3 cut(s)	343, 371, 1412
RseI	CAYNNNNRTG	3 cut(s)	512, 1656, 1757
SapI	GCTCTTC	1 cut(s)	1779
SaqAI	TTAA	2 cut(s)	1454, 1799
SatI	GCNGC	8 cut(s)	29, 32, 94, 140, 687, 1022, 1049, 1750
Sau3AI	GATC	7 cut(s)	269, 376, 699, 705, 1088, 1292, 1383
Sau96I	GGNCC	2 cut(s)	768, 1616
ScaI	AGTACT	1 cut(s)	372
SchI	GAGTC	4 cut(s)	1158, 1641, 1661, 1706
ScrFI	CCNGG	1 cut(s)	1614
SduI	GDGCHC	2 cut(s)	639, 1464
SfaNI	GCATC	5 cut(s)	32, 105, 289, 443, 744
SfcI	CTRYAG	2 cut(s)	1038, 1296
SfuI	TTCGAA	1 cut(s)	240
SmiMI	CAYNNNNRTG	3 cut(s)	512, 1656, 1757
SmlI	CTYRAG	4 cut(s)	143, 987, 1025, 1465
SmoI	CTYRAG	4 cut(s)	143, 987, 1025, 1465
SsiI	CCGC	6 cut(s)	29, 60, 689, 812, 1766, 1813
SspI	AATATT	2 cut(s)	1199, 1443
SspMI	CTAG	3 cut(s)	563, 1259, 1289
StyD4I	CCNGG	1 cut(s)	1612
StyI	CCWWGG	3 cut(s)	1077, 1288, 1570
TaaI	ACNGT	8 cut(s)	775, 790, 1123, 1168, 1351, 1416, 1503, 1756
TaiI	ACGT	2 cut(s)	432, 755
TaqI	TCGA	9 cut(s)	123, 240, 297, 520, 598, 723, 1458, 1670, 1782
TatI	WGTACW	1 cut(s)	370
TauI	GCSGC	1 cut(s)	31
TfiI	GAWTC	1 cut(s)	465
Tru1I	TTAA	2 cut(s)	1454, 1799
Tru9I	TTAA	2 cut(s)	1454, 1799
TscAI	CASTG	3 cut(s)	1356, 1566, 1707
TseFI	GTSAC	3 cut(s)	775, 1416, 1664
TseI	GCWGC	7 cut(s)	31, 93, 139, 686, 1021, 1048, 1749
Tsp45I	GTSAC	3 cut(s)	775, 1416, 1664
TspDTI	ATGAA	8 cut(s)	141, 440, 496, 922, 1202, 1440, 1674, 1676
TspGWI	ACGGA	1 cut(s)	1659
TspRI	CASTG	3 cut(s)	1356, 1566, 1707
Van91I	CCANNNNNTGG	1 cut(s)	765
VneI	GTGCAC	1 cut(s)	635
XagI	CCTNNNNNAGG	1 cut(s)	1076
XapI	RAATTY	3 cut(s)	73, 149, 548
XceI	RCATGY	1 cut(s)	812
XmaJI	CCTAGG	1 cut(s)	1288
XmnI	GAANNNNTTC	2 cut(s)	1200, 1710
XspI	CTAG	3 cut(s)	563, 1259, 1289
ZrmI	AGTACT	1 cut(s)	372
Zsp2I	ATGCAT	2 cut(s)	304, 1798

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.