Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0009077

Physical Location & Seq

Genomic Coordinates Reverse (-)

23401242 .. 23405988

Gene Structure 4747 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g30280.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1887 bp

ATGTCGTCGTCGTTCCTAACGGCGTCGCCGCTGCCTCTGCTGTTCCGAAGGCGGTCGAGCGGCGAGATCAGGAACTTGGCCTCGGTTTCGAGCAGCATATTGCCCGCTTTTGGAACCGTCGTCGATGATGGCCACTTGCAAACCAAGGGATTTGTTGTTGCTCCCTACGATAGGAGATACCGGTGGTGGCAGACGTTCTTGGTGGTGCTGGTGGTGTACTCAGCATGGGCGTCGCCGTTCGAGTTGGCTTTCAAAAAGGTGGCGACGGGATCTCTCATGCCGGTGGATTTGGTGGTCGATGCCTTCTTCGCCGTCGATATAGCACTCACTTTCTTTGTGGCTTACTTGGACAAGTCAACCTACCTCTTGGTGCTTGATCATAAGAAAATTGCTTCCAGGTACATTACAAAATATTACTTCGCAATGGACGTAGCCTCGACTGTACCGTTTCAAGCCCTTCACAGACTCTTCACTGGCCATACTCGAGAGGGCCTCGTCTTTGGCATCCTCAATCTGCTTCGTCTCTGGCGTCTCCGCCGCGTCAGTGAACTCTTTAAAAGACTAGAGAAAGACACTCGCTTTAGTTACTTTTGGGTCAGATACTTCAAACTCATTTGTGTTACACTATTTGCAGTGCACTCGGCAGGTTGTTTCTACTACTGGATGGCCACCCATCACAAAGCCTCAGCTGATACATGGATTGGAACTGAGGTCAATAATTTTGAACACAGAAGCATCTGGCTAGGTTACACATATGCCATATACTGGGCCACTGTCACCCTCACCACTGTTGGCTATGGTGACTTGCACGCAGTTAACTTTGGGGAGAAGATTTTCAGCATCTTCTATATGCTTTTCAACATTGGACTCTTTGCTTATTTGGTTGGTAACATGACCAATCTTGTTGTCCACAGTGCTTTCCGGACATTGTTCATGAGGGACTCTATCAATGAGATATTCAGATATGCAAGCAAGAACAGACTTCCAGAAGGCTTGAAAGAGCAAATGCTTGCCCACATGCAACTCAAGTTCAAGACAGCAGAGTTACAGCAGGAAGAAGTTCTACAAGACCTTCCGAAAGCAATAAGATCCAGCATTGCGCACCATCTTTTCCGGAAAACCATTGATAAAACCTACCTATTTAAAGGAATTTCTGAAGAGCTTACCGTTCAATTGGTCTCAGAGATGAAAGCAGAATATTTTCCTCCCAAAGTTGACATTATCTTGCAAAATGAAATTCCAACAGACTTTTACATTCTAGTAACCGGAGCTTTGGACATGCTAGTATACAAGAACGGCACAGAACAGTTCTTGTCAACTCTAGGATCTGCAGACATGGTAGGAGAGTTAGGGGTGTTTTTCAATATCCCACAACCATTTACAGTGAGAACAAAAAGGCTTTCTCAGGTTATACGCATCAGTCATCAGCATTTCAAGCAAATGATTCAGCCACAGAATGAAGACTCAAAGATAATCATCACAAACTTTACTCAGCACTTGAAGGACTTAAAACAGGAGGTGTTACAAGAAATTCCATTTCTACCAGAATTGCTGGAAGAACTTAATACAGAGCAGCTGGGTATGGAATCAAATGAAGATGCACGAAATCATGAACCAGTGGATAACCATGGAGATCCAATTGTGGAAGGAACAAACAAAACTAGTGCTCCGGCGCCAATTACCATGAGAGTGATAATTCATGGCCATCATCCAAATGAAGGTGACTCCAAAGGAAAACTAATTCATCTGCCTGATTCTATAGACAACCTTCTGAGATTAGCAGAGAAAAAGTTGGAAAAAAGAGGAAGCACAATTGTCATGGCTGACGGCTCAGTGGTTGAAGAACTGAATACTTTGAGAGATAACGATCACTTGTTTATCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009605 GO:0009607 GO:0009624 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ion_trans KHA cNMP_binding

Protein Analysis

629

Amino Acids

72.13

Weight (kDa)

7.12

Isoelectric Point (pI)

39.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	60 - 303	1.8e-38	Ion transport protein
Ion_trans_2	PF07885	222 - 302	5.4e-13	Ion channel
cNMP_binding	PF00027	400 - 483	1.1e-14	Cyclic nucleotide-binding domain
KHA	PF11834	563 - 627	3.3e-21	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009077)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32650 AT4G32650 AT4G32650
fragaria_vesca	FvH4_2g30280 FvH4_2g30280
malus_domestica	MD08G1134700.v1.1 MD15G1113600.v1.1
prunus_persica	Prupe.1G464600_v2.0.a1 Prupe.1G464600_v2.0.a1
pyrus_communis	pycom08g11370
rosa_chinensis	RchiOBHm_Chr6g0312591
rosa_laevigata	RLG00000010313
rosa_multiflora	Rmu_co8350831.1_g000001 Rmu_sc0003094.1_g000003 Rmu_sc0008363.1_g000002
rosa_roxburghii	Rroxscaffold_7G00156670
rosa_rugosa	Rorug06G0396700
rosa_samantha	Rh6AG514600 Rh6BG525400 Rh6CG530600 Rh6DG516700
rosa_wichuraiana	Rw6G044710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1101
Acc36I	ACCTGC	1 cut(s)	635
AccB1I	GGYRCC	1 cut(s)	1672
AccB7I	CCANNNNNTGG	1 cut(s)	765
AccBSI	CCGCTC	1 cut(s)	60
AccI	GTMKAC	1 cut(s)	1287
AccII	CGCG	1 cut(s)	540
AccIII	TCCGGA	2 cut(s)	921, 1113
AciI	CCGC	6 cut(s)	29, 52, 60, 105, 535, 538
AclWI	GGATC	4 cut(s)	277, 1083, 1333, 1628
AcoI	YGGCCR	4 cut(s)	130, 475, 666, 1702
AcsI	RAATTY	3 cut(s)	1149, 1236, 1530
AcuI	CTGAAG	1 cut(s)	1176
AcyI	GRCGYC	4 cut(s)	23, 230, 529, 1673
AfaI	GTAC	3 cut(s)	218, 401, 444
AfiI	CCNNNNNNNGG	4 cut(s)	110, 171, 765, 1718
AgeI	ACCGGT	1 cut(s)	180
AhlI	ACTAGT	1 cut(s)	1661
AjnI	CCWGG	1 cut(s)	395
AluBI	AGCT	4 cut(s)	689, 1162, 1271, 1576
AluI	AGCT	4 cut(s)	689, 1162, 1271, 1576
Alw21I	GWGCWC	2 cut(s)	639, 1669
Alw26I	GTCTC	3 cut(s)	527, 536, 1183
Alw44I	GTGCAC	1 cut(s)	635
AlwI	GGATC	4 cut(s)	277, 1083, 1333, 1628
Ama87I	CYCGRG	1 cut(s)	483
Aor13HI	TCCGGA	2 cut(s)	921, 1113
AoxI	GGCC	7 cut(s)	78, 130, 475, 490, 666, 768, 1702
ApaLI	GTGCAC	1 cut(s)	635
ApeKI	GCWGC	3 cut(s)	31, 93, 1573
ApoI	RAATTY	3 cut(s)	1149, 1236, 1530
AsiGI	ACCGGT	1 cut(s)	180
Asp700I	GAANNNNTTC	3 cut(s)	833, 1059, 1200
AspLEI	GCGC	2 cut(s)	1102, 1675
AspS9I	GGNCC	2 cut(s)	490, 768
AsuHPI	GGTGA	4 cut(s)	769, 775, 812, 1733
AvaI	CYCGRG	1 cut(s)	483
BaeGI	GKGCMC	1 cut(s)	639
BalI	TGGCCA	4 cut(s)	132, 477, 668, 1704
BanI	GGYRCC	1 cut(s)	1672
BarI	GAAGNNNNNNTAC	2 cut(s)	1047, 1079
BbsI	GAAGAC	1 cut(s)	1467
Bbv12I	GWGCWC	2 cut(s)	639, 1669
BbvCI	CCTCAGC	1 cut(s)	685
BbvI	GCAGC	3 cut(s)	18, 105, 1585
BccI	CCATC	5 cut(s)	122, 658, 681, 1113, 1713
BceAI	ACGGC	5 cut(s)	36, 220, 296, 1312, 1843
BcgI	CGANNNNNNTGC	4 cut(s)	213, 247, 621, 655
BciT130I	CCWGG	1 cut(s)	397
BclI	TGATCA	1 cut(s)	376
BcoDI	GTCTC	3 cut(s)	527, 536, 1183
BcuI	ACTAGT	1 cut(s)	1661
BfaI	CTAG	6 cut(s)	563, 743, 1259, 1283, 1322, 1662
BfmI	CTRYAG	2 cut(s)	1329, 1758
BfoI	RGCGCY	1 cut(s)	1676
BfuAI	ACCTGC	1 cut(s)	635
BisI	GCNGC	6 cut(s)	29, 32, 61, 94, 538, 1574
BlsI	GCNGC	6 cut(s)	30, 33, 62, 95, 539, 1575
Bme1390I	CCNGG	1 cut(s)	397
BmeT110I	CYCGRG	1 cut(s)	483
BmgT120I	GGNCC	2 cut(s)	490, 768
BmiI	GGNNCC	2 cut(s)	115, 1674
BmrFI	CCNGG	1 cut(s)	397
BmrI	ACTGGG	1 cut(s)	775
BmsI	GCATC	6 cut(s)	289, 513, 744, 849, 1425, 1588
BmuI	ACTGGG	1 cut(s)	775
BpiI	GAAGAC	1 cut(s)	1467
BplI	GAGNNNNNCTC	2 cut(s)	477, 509
Bpu10I	CCTNAGC	1 cut(s)	685
BpuEI	CTTGAG	1 cut(s)	1010
BsaBI	GATNNNNATC	2 cut(s)	1475, 1866
BsaHI	GRCGYC	4 cut(s)	23, 230, 529, 1673
BsaI	GGTCTC	1 cut(s)	1183
BsaJI	CCNNGG	3 cut(s)	81, 144, 1627
BsaWI	WCCGGW	4 cut(s)	180, 921, 1113, 1265
BsaXI	ACNNNNNCTCC	2 cut(s)	1651, 1681
Bsc4I	CCNNNNNNNGG	4 cut(s)	110, 171, 765, 1718
Bse118I	RCCGGY	2 cut(s)	180, 280
Bse1I	ACTGG	4 cut(s)	478, 665, 770, 1616
Bse3DI	GCAATG	2 cut(s)	429, 1095
Bse8I	GATNNNNATC	2 cut(s)	1475, 1866
BseAI	TCCGGA	2 cut(s)	921, 1113
BseBI	CCWGG	1 cut(s)	397
BseDI	CCNNGG	3 cut(s)	81, 144, 1627
BseGI	GGATG	3 cut(s)	504, 669, 1708
BseJI	GATNNNNATC	2 cut(s)	1475, 1866
BseLI	CCNNNNNNNGG	4 cut(s)	110, 171, 765, 1718
BseMI	GCAATG	2 cut(s)	429, 1095
BseMII	CTCAG	8 cut(s)	234, 699, 699, 1194, 1418, 1505, 1763, 1845
BseNI	ACTGG	4 cut(s)	478, 665, 770, 1616
BseSI	GKGCMC	1 cut(s)	639
BseXI	GCAGC	3 cut(s)	18, 105, 1585
BseYI	CCCAGC	1 cut(s)	1576
Bsh1236I	CGCG	1 cut(s)	540
Bsh1285I	CGRYCG	1 cut(s)	56
BshFI	GGCC	7 cut(s)	80, 132, 477, 492, 668, 770, 1704
BshNI	GGYRCC	1 cut(s)	1672
BshTI	ACCGGT	1 cut(s)	180
BsiEI	CGRYCG	1 cut(s)	56
BsiHKAI	GWGCWC	2 cut(s)	639, 1669
BsiHKCI	CYCGRG	1 cut(s)	483
BsiSI	CCGG	6 cut(s)	181, 281, 922, 1114, 1266, 1670
BslFI	GGGAC	1 cut(s)	953
BslI	CCNNNNNNNGG	4 cut(s)	110, 171, 765, 1718
BsmAI	GTCTC	3 cut(s)	527, 536, 1183
BsmBI	CGTCTC	2 cut(s)	527, 536
BsmFI	GGGAC	1 cut(s)	953
BsnI	GGCC	7 cut(s)	80, 132, 477, 492, 668, 770, 1704
Bso31I	GGTCTC	1 cut(s)	1183
BsoBI	CYCGRG	1 cut(s)	483
Bsp1286I	GDGCHC	2 cut(s)	639, 1669
Bsp13I	TCCGGA	2 cut(s)	921, 1113
Bsp143I	GATC	7 cut(s)	66, 269, 376, 1088, 1325, 1633, 1867
Bsp19I	CCATGG	1 cut(s)	1627
BspACI	CCGC	6 cut(s)	29, 52, 60, 105, 535, 538
BspANI	GGCC	7 cut(s)	80, 132, 477, 492, 668, 770, 1704
BspCNI	CTCAG	8 cut(s)	233, 698, 700, 1193, 1417, 1504, 1764, 1844
BspEI	TCCGGA	2 cut(s)	921, 1113
BspFNI	CGCG	1 cut(s)	540
BspHI	TCATGA	2 cut(s)	933, 1609
BspLI	GGNNCC	2 cut(s)	115, 1674
BspMAI	CTGCAG	1 cut(s)	1333
BspMI	ACCTGC	1 cut(s)	635
BspPI	GGATC	4 cut(s)	277, 1083, 1333, 1628
BspQI	GCTCTTC	1 cut(s)	1152
BspT107I	GGYRCC	1 cut(s)	1672
BspTNI	GGTCTC	1 cut(s)	1183
BsrBI	CCGCTC	1 cut(s)	60
BsrDI	GCAATG	2 cut(s)	429, 1095
BsrFI	RCCGGY	2 cut(s)	180, 280
BsrI	ACTGG	4 cut(s)	478, 665, 770, 1616
BssAI	RCCGGY	2 cut(s)	180, 280
BssECI	CCNNGG	3 cut(s)	81, 144, 1627
BssMI	GATC	7 cut(s)	66, 269, 376, 1088, 1325, 1633, 1867
BssNAI	GTATAC	1 cut(s)	1288
BssNI	GRCGYC	4 cut(s)	23, 230, 529, 1673
BssT1I	CCWWGG	2 cut(s)	144, 1627
Bst1107I	GTATAC	1 cut(s)	1288
Bst2UI	CCWGG	1 cut(s)	397
Bst4CI	ACNGT	9 cut(s)	118, 442, 447, 775, 790, 914, 1168, 1308, 1384
Bst6I	CTCTTC	2 cut(s)	473, 1152
BstACI	GRCGYC	4 cut(s)	23, 230, 529, 1673
BstC8I	GCNNGC	4 cut(s)	105, 810, 970, 1011
BstDEI	CTNAG	8 cut(s)	220, 685, 708, 1180, 1404, 1491, 1772, 1831
BstDSI	CCRYGG	1 cut(s)	1627
BstENI	CCTNNNNNAGG	1 cut(s)	169
BstF5I	GGATG	3 cut(s)	504, 669, 1708
BstFNI	CGCG	1 cut(s)	540
BstH2I	RGCGCY	1 cut(s)	1676
BstHHI	GCGC	2 cut(s)	1102, 1675
BstKTI	GATC	7 cut(s)	69, 272, 379, 1091, 1328, 1636, 1870
BstMAI	GTCTC	3 cut(s)	527, 536, 1183
BstMBI	GATC	7 cut(s)	66, 269, 376, 1088, 1325, 1633, 1867
BstMCI	CGRYCG	1 cut(s)	56
BstMWI	GCNNNNNNNGC	3 cut(s)	37, 308, 1435
BstNI	CCWGG	1 cut(s)	397
BstNSI	RCATGY	2 cut(s)	1021, 1282
BstSCI	CCNGG	1 cut(s)	395
BstSFI	CTRYAG	2 cut(s)	1329, 1758
BstSLI	GKGCMC	1 cut(s)	639
BstUI	CGCG	1 cut(s)	540
BstV1I	GCAGC	3 cut(s)	18, 105, 1585
BstV2I	GAAGAC	1 cut(s)	1467
BstX2I	RGATCY	4 cut(s)	269, 1088, 1325, 1633
BstYI	RGATCY	4 cut(s)	269, 1088, 1325, 1633
BstZ17I	GTATAC	1 cut(s)	1288
BsuRI	GGCC	7 cut(s)	80, 132, 477, 492, 668, 770, 1704
BtgI	CCRYGG	1 cut(s)	1627
BtsCI	GGATG	3 cut(s)	504, 669, 1708
BtsI	GCAGTG	1 cut(s)	639
BtsIMutI	CAGTG	9 cut(s)	471, 550, 639, 771, 786, 919, 1389, 1623, 1839
BveI	ACCTGC	1 cut(s)	635
Cac8I	GCNNGC	4 cut(s)	105, 810, 970, 1011
CciI	TCATGA	2 cut(s)	933, 1609
CfoI	GCGC	2 cut(s)	1102, 1675
Cfr10I	RCCGGY	2 cut(s)	180, 280
Cfr13I	GGNCC	2 cut(s)	490, 768
CseI	GACGC	4 cut(s)	12, 219, 518, 529
Csp6I	GTAC	3 cut(s)	217, 400, 443
CspAI	ACCGGT	1 cut(s)	180
CviQI	GTAC	3 cut(s)	217, 400, 443
DdeI	CTNAG	8 cut(s)	220, 685, 708, 1180, 1404, 1491, 1772, 1831
DinI	GGCGCC	1 cut(s)	1674
DpnI	GATC	7 cut(s)	68, 271, 378, 1090, 1327, 1635, 1869
DpnII	GATC	7 cut(s)	66, 269, 376, 1088, 1325, 1633, 1867
DraI	TTTAAA	2 cut(s)	556, 1144
EaeI	YGGCCR	4 cut(s)	130, 475, 666, 1702
Eam1104I	CTCTTC	2 cut(s)	473, 1152
EarI	CTCTTC	2 cut(s)	473, 1152
EciI	GGCGGA	1 cut(s)	524
Eco130I	CCWWGG	2 cut(s)	144, 1627
Eco31I	GGTCTC	1 cut(s)	1183
Eco57I	CTGAAG	1 cut(s)	1176
Eco88I	CYCGRG	1 cut(s)	483
EcoNI	CCTNNNNNAGG	1 cut(s)	169
EcoO109I	RGGNCCY	1 cut(s)	490
EcoRII	CCWGG	1 cut(s)	395
EcoT14I	CCWWGG	2 cut(s)	144, 1627
EgeI	GGCGCC	1 cut(s)	1674
EheI	GGCGCC	1 cut(s)	1674
ErhI	CCWWGG	2 cut(s)	144, 1627
Esp3I	CGTCTC	2 cut(s)	527, 536
FaqI	GGGAC	1 cut(s)	953
FauI	CCCGC	1 cut(s)	112
FauNDI	CATATG	1 cut(s)	754
FbaI	TGATCA	1 cut(s)	376
FblI	GTMKAC	1 cut(s)	1287
Fnu4HI	GCNGC	6 cut(s)	29, 32, 61, 94, 538, 1574
FokI	GGATG	3 cut(s)	491, 676, 1695
Fsp4HI	GCNGC	6 cut(s)	29, 32, 61, 94, 538, 1574
FspBI	CTAG	6 cut(s)	563, 743, 1259, 1283, 1322, 1662
FspI	TGCGCA	1 cut(s)	1101
GlaI	GCGC	2 cut(s)	1101, 1674
GluI	GCNGC	6 cut(s)	29, 32, 61, 94, 538, 1574
GsaI	CCCAGC	1 cut(s)	1580
HaeII	RGCGCY	1 cut(s)	1676
HaeIII	GGCC	7 cut(s)	80, 132, 477, 492, 668, 770, 1704
HapII	CCGG	6 cut(s)	181, 281, 922, 1114, 1266, 1670
HgaI	GACGC	4 cut(s)	12, 219, 518, 529
HhaI	GCGC	2 cut(s)	1102, 1675
Hin1I	GRCGYC	4 cut(s)	23, 230, 529, 1673
Hin6I	GCGC	2 cut(s)	1100, 1673
HinP1I	GCGC	2 cut(s)	1100, 1673
HincII	GTYRAC	4 cut(s)	357, 817, 1216, 1317
HindII	GTYRAC	4 cut(s)	357, 817, 1216, 1317
HinfI	GANTC	8 cut(s)	465, 867, 941, 1444, 1463, 1586, 1724, 1754
HpaI	GTTAAC	1 cut(s)	817
HpaII	CCGG	6 cut(s)	181, 281, 922, 1114, 1266, 1670
HphI	GGTGA	4 cut(s)	769, 775, 812, 1733
Hpy166II	GTNNAC	9 cut(s)	217, 357, 548, 637, 817, 910, 1216, 1288, 1317
Hpy188I	TCNGA	7 cut(s)	47, 599, 962, 1077, 1156, 1183, 1773
Hpy188III	TCNNGA	8 cut(s)	70, 485, 922, 934, 986, 1033, 1114, 1610
Hpy8I	GTNNAC	9 cut(s)	217, 357, 548, 637, 817, 910, 1216, 1288, 1317
Hpy99I	CGWCG	8 cut(s)	10, 13, 28, 122, 125, 235, 268, 317
HpyAV	CCTTC	9 cut(s)	42, 313, 467, 983, 1082, 1495, 1640, 1712, 1778
HpyCH4III	ACNGT	9 cut(s)	118, 442, 447, 775, 790, 914, 1168, 1308, 1384
HpyCH4IV	ACGT	2 cut(s)	194, 429
HpyCH4V	TGCA	9 cut(s)	139, 632, 637, 808, 968, 1021, 1228, 1331, 1601
HpyF10VI	GCNNNNNNNGC	3 cut(s)	37, 308, 1435
HpyF3I	CTNAG	8 cut(s)	220, 685, 708, 1180, 1404, 1491, 1772, 1831
HpySE526I	ACGT	2 cut(s)	194, 429
Hsp92I	GRCGYC	4 cut(s)	23, 230, 529, 1673
HspAI	GCGC	2 cut(s)	1100, 1673
KasI	GGCGCC	1 cut(s)	1672
Kpn2I	TCCGGA	2 cut(s)	921, 1113
Ksp22I	TGATCA	1 cut(s)	376
KspAI	GTTAAC	1 cut(s)	817
Kzo9I	GATC	7 cut(s)	66, 269, 376, 1088, 1325, 1633, 1867
LguI	GCTCTTC	1 cut(s)	1152
LmnI	GCTCC	3 cut(s)	166, 1268, 1672
Lsp1109I	GCAGC	3 cut(s)	18, 105, 1585
LweI	GCATC	6 cut(s)	289, 513, 744, 849, 1425, 1588
MaeI	CTAG	6 cut(s)	563, 743, 1259, 1283, 1322, 1662
MaeII	ACGT	2 cut(s)	194, 429
MalI	GATC	7 cut(s)	68, 271, 378, 1090, 1327, 1635, 1869
MbiI	CCGCTC	1 cut(s)	60
MboI	GATC	7 cut(s)	66, 269, 376, 1088, 1325, 1633, 1867
MfeI	CAATTG	3 cut(s)	1172, 1638, 1812
MflI	RGATCY	4 cut(s)	269, 1088, 1325, 1633
MhlI	GDGCHC	2 cut(s)	639, 1669
MlsI	TGGCCA	4 cut(s)	132, 477, 668, 1704
MluNI	TGGCCA	4 cut(s)	132, 477, 668, 1704
Mly113I	GGCGCC	1 cut(s)	1673
MlyI	GAGTC	5 cut(s)	459, 861, 935, 1457, 1718
MmeI	TCCRAC	2 cut(s)	1265, 1773
Mox20I	TGGCCA	4 cut(s)	132, 477, 668, 1704
MroI	TCCGGA	2 cut(s)	921, 1113
MroXI	GAANNNNTTC	3 cut(s)	833, 1059, 1200
MscI	TGGCCA	4 cut(s)	132, 477, 668, 1704
MseI	TTAA	5 cut(s)	555, 816, 1143, 1508, 1563
MslI	CAYNNNNRTG	3 cut(s)	281, 1688, 1713
Msp20I	TGGCCA	4 cut(s)	132, 477, 668, 1704
MspA1I	CMGCKG	3 cut(s)	31, 689, 1576
MspI	CCGG	6 cut(s)	181, 281, 922, 1114, 1266, 1670
MspR9I	CCNGG	1 cut(s)	397
MunI	CAATTG	3 cut(s)	1172, 1638, 1812
MvaI	CCWGG	1 cut(s)	397
MvnI	CGCG	1 cut(s)	540
MwoI	GCNNNNNNNGC	3 cut(s)	37, 308, 1435
NarI	GGCGCC	1 cut(s)	1673
NcoI	CCATGG	1 cut(s)	1627
NdeI	CATATG	1 cut(s)	754
NdeII	GATC	7 cut(s)	66, 269, 376, 1088, 1325, 1633, 1867
NlaIV	GGNNCC	2 cut(s)	115, 1674
NmeAIII	GCCGAG	1 cut(s)	620
NmuCI	GTSAC	3 cut(s)	775, 800, 1721
NsbI	TGCGCA	1 cut(s)	1101
NspI	RCATGY	2 cut(s)	1021, 1282
PaeR7I	CTCGAG	1 cut(s)	483
PagI	TCATGA	2 cut(s)	933, 1609
PciSI	GCTCTTC	1 cut(s)	1152
PcsI	WCGNNNNNNNCGW	3 cut(s)	426, 443, 526
PdmI	GAANNNNTTC	3 cut(s)	833, 1059, 1200
PfeI	GAWTC	3 cut(s)	1444, 1586, 1754
PflMI	CCANNNNNTGG	1 cut(s)	765
PinAI	ACCGGT	1 cut(s)	180
PkrI	GCNGC	6 cut(s)	30, 33, 62, 95, 539, 1575
PleI	GAGTC	5 cut(s)	459, 861, 935, 1457, 1718
PluTI	GGCGCC	1 cut(s)	1676
PpsI	GAGTC	5 cut(s)	459, 861, 935, 1457, 1718
Psp6I	CCWGG	1 cut(s)	395
PspFI	CCCAGC	1 cut(s)	1576
PspGI	CCWGG	1 cut(s)	395
PspN4I	GGNNCC	2 cut(s)	115, 1674
PspPI	GGNCC	2 cut(s)	490, 768
PstI	CTGCAG	1 cut(s)	1333
PsuI	RGATCY	4 cut(s)	269, 1088, 1325, 1633
PvuII	CAGCTG	2 cut(s)	689, 1576
RsaI	GTAC	3 cut(s)	218, 401, 444
RsaNI	GTAC	3 cut(s)	217, 400, 443
RseI	CAYNNNNRTG	3 cut(s)	281, 1688, 1713
SapI	GCTCTTC	1 cut(s)	1152
SaqAI	TTAA	5 cut(s)	555, 816, 1143, 1508, 1563
SatI	GCNGC	6 cut(s)	29, 32, 61, 94, 538, 1574
Sau3AI	GATC	7 cut(s)	66, 269, 376, 1088, 1325, 1633, 1867
Sau96I	GGNCC	2 cut(s)	490, 768
SchI	GAGTC	5 cut(s)	459, 861, 935, 1457, 1718
ScrFI	CCNGG	1 cut(s)	397
SduI	GDGCHC	2 cut(s)	639, 1669
SfaNI	GCATC	6 cut(s)	289, 513, 744, 849, 1425, 1588
SfcI	CTRYAG	2 cut(s)	1329, 1758
SfoI	GGCGCC	1 cut(s)	1674
Sfr274I	CTCGAG	1 cut(s)	483
SlaI	CTCGAG	1 cut(s)	483
SmiMI	CAYNNNNRTG	3 cut(s)	281, 1688, 1713
SmlI	CTYRAG	2 cut(s)	483, 1025
SmoI	CTYRAG	2 cut(s)	483, 1025
SpeI	ACTAGT	1 cut(s)	1661
SsiI	CCGC	6 cut(s)	29, 52, 60, 105, 535, 538
SspDI	GGCGCC	1 cut(s)	1672
SspI	AATATT	2 cut(s)	413, 1199
SspMI	CTAG	6 cut(s)	563, 743, 1259, 1283, 1322, 1662
StyD4I	CCNGG	1 cut(s)	395
StyI	CCWWGG	2 cut(s)	144, 1627
TaaI	ACNGT	9 cut(s)	118, 442, 447, 775, 790, 914, 1168, 1308, 1384
TaiI	ACGT	2 cut(s)	197, 432
TaqI	TCGA	8 cut(s)	56, 89, 123, 240, 297, 315, 437, 484
TatI	WGTACW	1 cut(s)	216
TauI	GCSGC	3 cut(s)	31, 63, 540
TfiI	GAWTC	3 cut(s)	1444, 1586, 1754
Tru1I	TTAA	5 cut(s)	555, 816, 1143, 1508, 1563
Tru9I	TTAA	5 cut(s)	555, 816, 1143, 1508, 1563
TscAI	CASTG	9 cut(s)	478, 550, 639, 778, 793, 919, 1389, 1623, 1839
TseFI	GTSAC	3 cut(s)	775, 800, 1721
TseI	GCWGC	3 cut(s)	31, 93, 1573
Tsp45I	GTSAC	3 cut(s)	775, 800, 1721
TspDTI	ATGAA	9 cut(s)	922, 1202, 1248, 1473, 1608, 1626, 1688, 1731, 1733
TspRI	CASTG	9 cut(s)	478, 550, 639, 778, 793, 919, 1389, 1623, 1839
Van91I	CCANNNNNTGG	1 cut(s)	765
VneI	GTGCAC	1 cut(s)	635
XagI	CCTNNNNNAGG	1 cut(s)	169
XapI	RAATTY	3 cut(s)	1149, 1236, 1530
XceI	RCATGY	2 cut(s)	1021, 1282
XhoI	CTCGAG	1 cut(s)	483
XmiI	GTMKAC	1 cut(s)	1287
XmnI	GAANNNNTTC	3 cut(s)	833, 1059, 1200
XspI	CTAG	6 cut(s)	563, 743, 1259, 1283, 1322, 1662

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.