AT4G32915

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015435
Physical Location & Seq
Forward (+)
15885520 .. 15887044
1525 bp
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UTR
Exon/CDS
Intron
AT4G32915.1

Sequence Viewer

Length: 468 bp
ATGGCGACCAGAGCTTTGCTCGCCGTTATATATGCGTCTCCGAATCGCTGCTACATCTCTCCGAGCAGGATCAAAATCCAATCTTTAACTTGTTCTTCTTCAAGTCATTATTACCAAAGACAGAGTAGAAAAAACCACAGAATAGCTCGAAGTTACTCGTCTGACTCGGATTCCTCCGTTCTTCAGCCGCCGGATGTGGCCCGTCTTGCTCAGACAGCTCGAATCTCTCTAACTCCGGCGGAGATAGAGGAATGTGAAACTAAAATTCGTCGAGTCATCGACTGGTTTGGGCAGCTTCAACAAGTTGATGTTAACAGTGTGGAGCCAGCTATTAGAGCAGAAATGGATGGTGGAAATTTGCGAGAGGATGCTCCAGAAACATTTGACAACAGGGACAGTATAAGAGCTTCCATTCCAAGCTTTGAGGACGCATATCTAAAAGTCCCTAAAATACTAAACAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
Glu-tRNAGln

Protein Analysis

155

Amino Acids

17.52

Weight (kDa)

7.71

Isoelectric Point (pI)

77.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GatC PF02686 77 - 148 3.2e-12 Glu-tRNAGln amidotransferase C subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32915
fragaria_vesca FvH4_2g35131
malus_domestica MD08G1108800.v1.1
prunus_persica Prupe.1G443400_v2.0.a1 Prupe.1G443400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306821
rosa_laevigata RLG00000010763
rosa_multiflora Rmu_sc0016250.1_g000001 Rmu_sc0027973.1_g000001
rosa_roxburghii Rroxscaffold_7G00161440
rosa_rugosa Rorug06G0353400
rosa_samantha Rh6AG465100 Rh6BG435700 Rh6CG479500 Rh6DG466400
rosa_wichuraiana Rw6G040530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 188, 239
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 2 cut(s) 264, 355
AcuI CTGAAG 1 cut(s) 167
AgsI TTSAA 2 cut(s) 102, 299
AluBI AGCT 7 cut(s) 14, 146, 218, 295, 329, 407, 420
AluI AGCT 7 cut(s) 14, 146, 218, 295, 329, 407, 420
Alw26I GTCTC 1 cut(s) 42
AlwI GGATC 1 cut(s) 77
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 2 cut(s) 48, 292
ApoI RAATTY 2 cut(s) 264, 355
ArsI GACNNNNNNTTYG 1 cut(s) 30
AspS9I GGNCC 1 cut(s) 199
BarI GAAGNNNNNNTAC 2 cut(s) 391, 423
BbvI GCAGC 2 cut(s) 35, 304
BccI CCATC 1 cut(s) 341
BceAI ACGGC 1 cut(s) 8
BcoDI GTCTC 1 cut(s) 42
BisI GCNGC 3 cut(s) 49, 188, 293
BlsI GCNGC 3 cut(s) 50, 189, 294
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 1 cut(s) 324
BmsI GCATC 1 cut(s) 358
BplI GAGNNNNNCTC 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 357
BsaBI GATNNNNATC 1 cut(s) 74
Bse1I ACTGG 1 cut(s) 287
Bse8I GATNNNNATC 1 cut(s) 74
BseGI GGATG 3 cut(s) 199, 352, 373
BseJI GATNNNNATC 1 cut(s) 74
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 1 cut(s) 287
BseXI GCAGC 2 cut(s) 35, 304
BshFI GGCC 1 cut(s) 200
BsiSI CCGG 2 cut(s) 191, 236
BslFI GGGAC 2 cut(s) 407, 428
BsmAI GTCTC 1 cut(s) 42
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 2 cut(s) 407, 428
BsnI GGCC 1 cut(s) 200
Bsp143I GATC 1 cut(s) 69
BspACI CCGC 2 cut(s) 188, 239
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 324
BspPI GGATC 1 cut(s) 77
BsrI ACTGG 1 cut(s) 287
BssMI GATC 1 cut(s) 69
Bst4CI ACNGT 2 cut(s) 317, 398
BstC8I GCNNGC 2 cut(s) 21, 327
BstDEI CTNAG 1 cut(s) 210
BstF5I GGATG 3 cut(s) 199, 352, 373
BstKTI GATC 1 cut(s) 72
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 206, 215, 335
BstV1I GCAGC 2 cut(s) 35, 304
BsuRI GGCC 1 cut(s) 200
BtsCI GGATG 3 cut(s) 199, 352, 373
BtsIMutI CAGTG 1 cut(s) 322
Cac8I GCNNGC 2 cut(s) 21, 327
Cfr13I GGNCC 1 cut(s) 199
CseI GACGC 2 cut(s) 24, 437
DdeI CTNAG 1 cut(s) 210
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 254
Eco57I CTGAAG 1 cut(s) 167
Esp3I CGTCTC 1 cut(s) 42
FaiI YATR 5 cut(s) 29, 31, 33, 401, 433
FaqI GGGAC 2 cut(s) 407, 428
Fnu4HI GCNGC 3 cut(s) 49, 188, 293
FokI GGATG 3 cut(s) 206, 359, 380
Fsp4HI GCNGC 3 cut(s) 49, 188, 293
GluI GCNGC 3 cut(s) 49, 188, 293
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 1 cut(s) 200
HapII CCGG 2 cut(s) 191, 236
HgaI GACGC 2 cut(s) 24, 437
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 418
HinfI GANTC 5 cut(s) 43, 164, 170, 222, 273
HpaI GTTAAC 1 cut(s) 313
HpaII CCGG 2 cut(s) 191, 236
Hpy166II GTNNAC 1 cut(s) 313
Hpy188I TCNGA 5 cut(s) 42, 63, 163, 169, 213
Hpy188III TCNNGA 1 cut(s) 374
Hpy8I GTNNAC 1 cut(s) 313
Hpy99I CGWCG 1 cut(s) 273
HpyCH4III ACNGT 2 cut(s) 317, 398
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 206, 215, 335
HpyF3I CTNAG 1 cut(s) 210
KspAI GTTAAC 1 cut(s) 313
Kzo9I GATC 1 cut(s) 69
LmnI GCTCC 2 cut(s) 322, 376
LpnPI CCDG 8 cut(s) 22, 52, 204, 249, 268, 339, 376, 387
Lsp1109I GCAGC 2 cut(s) 35, 304
LweI GCATC 1 cut(s) 358
MaeIII GTNAC 1 cut(s) 152
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 3 cut(s) 87, 90, 173
MluCI AATT 2 cut(s) 264, 355
MlyI GAGTC 2 cut(s) 158, 282
MnlI CCTC 4 cut(s) 184, 241, 358, 418
MseI TTAA 2 cut(s) 86, 312
MspI CCGG 2 cut(s) 191, 236
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 206, 215, 335
NdeII GATC 1 cut(s) 69
NlaIV GGNNCC 1 cut(s) 324
PfeI GAWTC 3 cut(s) 43, 170, 222
PkrI GCNGC 3 cut(s) 50, 189, 294
PleI GAGTC 2 cut(s) 158, 281
PpsI GAGTC 2 cut(s) 158, 281
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 1 cut(s) 199
SaqAI TTAA 2 cut(s) 86, 312
SatI GCNGC 3 cut(s) 49, 188, 293
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 2 cut(s) 158, 282
SetI ASST 7 cut(s) 16, 148, 220, 297, 331, 409, 422
SfaNI GCATC 1 cut(s) 358
Sse9I AATT 2 cut(s) 264, 355
SsiI CCGC 2 cut(s) 188, 239
TaaI ACNGT 2 cut(s) 317, 398
TaqI TCGA 4 cut(s) 148, 220, 271, 279
TasI AATT 2 cut(s) 264, 355
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 3 cut(s) 43, 170, 222
Tru1I TTAA 2 cut(s) 86, 312
Tru9I TTAA 2 cut(s) 86, 312
TscAI CASTG 1 cut(s) 322
TseI GCWGC 2 cut(s) 48, 292
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 1 cut(s) 322
XapI RAATTY 2 cut(s) 264, 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.