FvH4_2g35131

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015435
Physical Location & Seq
Reverse (-)
25963502 .. 25964403
902 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35131.t1

Sequence Viewer

Length: 444 bp
ATGGGAAGTAGAGCTTTGCTTCTGGTGAAGGATGTGTCTTGTTGTCTGTTACCCAAGAACCAGCTTCTACCCAACTTCAACTGCAACCGGAAACTTCCACAAGTGCTTCACCGGCGGCGAAGTTTATCAACTGCCATAACTGCTGGGTCTTCTTCTCTGGAACCTCTGGATGTGCCACGTCTGGCTGAGACTGCTCGAATCTCTCTGACCCCACAAGAGGTTGAAGAATTTGCTCCCAAAATTGGACAAGTCATGGAATGGTTTGGACAGCTTCAAGATGTTGATCTTGAAAGTGTGGAGCCCTCTATAAGAGCAGATACTGAAGGTGATAATTTCCGCGCTGATGTCCCGGAAACATTTGAGAATAGGAAGGCCATAATTGATGCTATTCCAACTTATGATGAGCCATACATCAAAGTTCCCAAGGTCTTGAACAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
Glu-tRNAGln

Protein Analysis

148

Amino Acids

16.5

Weight (kDa)

5.14

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GatC PF02686 69 - 140 7.2e-13 Glu-tRNAGln amidotransferase C subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32915
fragaria_vesca FvH4_2g35131
malus_domestica MD08G1108800.v1.1
prunus_persica Prupe.1G443400_v2.0.a1 Prupe.1G443400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306821
rosa_laevigata RLG00000010763
rosa_multiflora Rmu_sc0016250.1_g000001 Rmu_sc0027973.1_g000001
rosa_roxburghii Rroxscaffold_7G00161440
rosa_rugosa Rorug06G0353400
rosa_samantha Rh6AG465100 Rh6BG435700 Rh6CG479500 Rh6DG466400
rosa_wichuraiana Rw6G040530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 339
AciI CCGC 2 cut(s) 115, 337
AcsI RAATTY 1 cut(s) 227
AcuI CTGAAG 1 cut(s) 342
AfiI CCNNNNNNNGG 2 cut(s) 217, 242
AgsI TTSAA 5 cut(s) 79, 224, 275, 290, 433
AjiI CACGTC 1 cut(s) 179
AluBI AGCT 3 cut(s) 14, 64, 271
AluI AGCT 3 cut(s) 14, 64, 271
Alw26I GTCTC 1 cut(s) 182
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 1 cut(s) 372
ApoI RAATTY 1 cut(s) 227
AspLEI GCGC 1 cut(s) 341
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 3 cut(s) 37, 101, 338
BanII GRGCYC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 141
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 1 cut(s) 182
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
Bme1390I CCNGG 1 cut(s) 350
BmgBI CACGTC 1 cut(s) 179
BmiI GGNNCC 2 cut(s) 162, 300
BmrFI CCNGG 1 cut(s) 350
BmsI GCATC 1 cut(s) 373
BpiI GAAGAC 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 350
BsaBI GATNNNNATC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 423
BsaWI WCCGGW 1 cut(s) 87
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 242
Bse118I RCCGGY 1 cut(s) 111
Bse8I GATNNNNATC 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 2 cut(s) 37, 175
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 2 cut(s) 217, 242
BseMII CTCAG 1 cut(s) 177
BseYI CCCAGC 1 cut(s) 143
Bsh1236I CGCG 1 cut(s) 339
BshFI GGCC 1 cut(s) 374
BsiSI CCGG 3 cut(s) 88, 112, 350
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 217, 242
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 2 cut(s) 115, 337
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 1 cut(s) 178
BspFNI CGCG 1 cut(s) 339
BspLI GGNNCC 2 cut(s) 162, 300
BsrFI RCCGGY 1 cut(s) 111
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 1 cut(s) 283
BssT1I CCWWGG 1 cut(s) 423
BstDEI CTNAG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 37, 175
BstFNI CGCG 1 cut(s) 339
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 1 cut(s) 286
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 1 cut(s) 283
BstMWI GCNNNNNNNGC 3 cut(s) 112, 140, 191
BstSCI CCNGG 1 cut(s) 348
BstUI CGCG 1 cut(s) 339
BstV2I GAAGAC 1 cut(s) 141
BsuRI GGCC 1 cut(s) 374
BtrI CACGTC 1 cut(s) 179
BtsCI GGATG 2 cut(s) 37, 175
CaiI CAGNNNCTG 1 cut(s) 320
CfoI GCGC 1 cut(s) 341
Cfr10I RCCGGY 1 cut(s) 111
CviAII CATG 1 cut(s) 253
CviJI RGCY 7 cut(s) 14, 64, 185, 271, 301, 374, 406
CviKI_1 RGCY 7 cut(s) 14, 64, 185, 271, 301, 374, 406
DdeI CTNAG 1 cut(s) 186
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 303
Eco57I CTGAAG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 1 cut(s) 256
FaiI YATR 6 cut(s) 137, 254, 308, 377, 399, 409
FalI AAGNNNNNCTT 1 cut(s) 30
FaqI GGGAC 1 cut(s) 332
FatI CATG 1 cut(s) 252
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 2 cut(s) 44, 182
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 1 cut(s) 116
GlaI GCGC 1 cut(s) 340
GluI GCNGC 1 cut(s) 116
GsaI CCCAGC 1 cut(s) 147
HaeIII GGCC 1 cut(s) 374
HapII CCGG 3 cut(s) 88, 112, 350
HhaI GCGC 1 cut(s) 341
Hin1II CATG 1 cut(s) 256
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HinfI GANTC 1 cut(s) 198
HpaII CCGG 3 cut(s) 88, 112, 350
HphI GGTGA 3 cut(s) 37, 101, 338
Hpy188I TCNGA 1 cut(s) 207
Hpy188III TCNNGA 5 cut(s) 158, 167, 275, 287, 430
HpyAV CCTTC 3 cut(s) 22, 317, 364
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 140, 191
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 1 cut(s) 256
HspAI GCGC 1 cut(s) 339
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 2 cut(s) 238, 298
LpnPI CCDG 9 cut(s) 8, 74, 101, 125, 129, 143, 152, 167, 363
LweI GCATC 1 cut(s) 373
MaeII ACGT 1 cut(s) 178
MaeIII GTNAC 1 cut(s) 48
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 3 cut(s) 141, 144, 236
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 4 cut(s) 227, 240, 331, 378
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 3 cut(s) 174, 211, 313
MspI CCGG 3 cut(s) 88, 112, 350
MspR9I CCNGG 1 cut(s) 350
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 3 cut(s) 112, 140, 191
NciI CCSGG 1 cut(s) 350
NdeII GATC 1 cut(s) 283
NlaIII CATG 1 cut(s) 256
NlaIV GGNNCC 2 cut(s) 162, 300
PfeI GAWTC 1 cut(s) 198
PfoI TCCNGGA 1 cut(s) 348
PkrI GCNGC 1 cut(s) 117
PspFI CCCAGC 1 cut(s) 143
PspN4I GGNNCC 2 cut(s) 162, 300
PstNI CAGNNNCTG 1 cut(s) 320
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 1 cut(s) 283
ScrFI CCNGG 1 cut(s) 350
SduI GDGCHC 1 cut(s) 303
SetI ASST 8 cut(s) 16, 66, 166, 181, 222, 273, 328, 429
SfaNI GCATC 1 cut(s) 373
SgrAI CRCCGGYG 1 cut(s) 111
Sse9I AATT 4 cut(s) 227, 240, 331, 378
SsiI CCGC 2 cut(s) 115, 337
StyD4I CCNGG 1 cut(s) 348
StyI CCWWGG 1 cut(s) 423
TaiI ACGT 1 cut(s) 181
TaqI TCGA 1 cut(s) 196
TasI AATT 4 cut(s) 227, 240, 331, 378
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 1 cut(s) 198
XapI RAATTY 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.