Rh6BG435700

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015435
Physical Location & Seq
Reverse (-)
65559058 .. 65560252
1195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG435700.1

Sequence Viewer

Length: 693 bp
ATGCAGCCAAAATTCTATACTCCTTGTGTTCCTTCTTTTTGGCCTAAAGAACACTTCTTCTTTGTTAGTATTTATTATTGCCTAATATTTAAGAGGAGGAGTAAGGATAAAACTGTCATTTCATTCTCAACTGGAACGGGTCGGGTGAAGAGAGAAACGGGTTATGCTTTTGAAAATTCAATCCTAGCTAACCCAACCCATATAGATAGGAAGTTCACAACAGAACTGAGAATCAGAAAAATGGGGAGCAGAGCTTTGCTTCTGGTCAAGGAGGTGTCTTCTTGTTTGTTACCCAAGAACCAGTTTTTACCCAACTTTAACTGCCACCGAAAATTTCCACAACTTCTTCACCGGCGGCGAAGTTTCTCAACTGCAACAACTGCTGGGTCTTCTTCTTCTTCTCCTCTGGAAGCTCTGGATGTGCCACGTCTGGCTGAGACTGCTCGAATTTCTCTGACCCCTCAAGAGGTTGAAGAATTTGCTCCCAAAATTGGACAAGTCATGGAATGGTTTGGACAGCTTCAAGCAGTTGATCTTGAAAGCGTGGAGCCCTCTATAAGAGCAGATACTGAAGGTGATAATTTCCGTGCTGATGTCCCCGAAACATTTGAGAATAGGAATGCCATAATTGCTGCTATTCCAAATTATGAGGAGCCATACATCAAGGTTCCCAAGGTCTTGAACAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
Glu-tRNAGln

Protein Analysis

230

Amino Acids

26.34

Weight (kDa)

9.16

Isoelectric Point (pI)

53.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GatC PF02686 152 - 223 2.2e-13 Glu-tRNAGln amidotransferase C subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32915
fragaria_vesca FvH4_2g35131
malus_domestica MD08G1108800.v1.1
prunus_persica Prupe.1G443400_v2.0.a1 Prupe.1G443400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306821
rosa_laevigata RLG00000010763
rosa_multiflora Rmu_sc0016250.1_g000001 Rmu_sc0027973.1_g000001
rosa_roxburghii Rroxscaffold_7G00161440
rosa_rugosa Rorug06G0353400
rosa_samantha Rh6AG465100 Rh6BG435700 Rh6CG479500 Rh6DG466400
rosa_wichuraiana Rw6G040530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 355
AcsI RAATTY 5 cut(s) 11, 175, 332, 447, 476
AcuI CTGAAG 1 cut(s) 591
AfiI CCNNNNNNNGG 2 cut(s) 466, 491
AgsI TTSAA 6 cut(s) 173, 180, 473, 524, 539, 682
AjiI CACGTC 1 cut(s) 428
AluBI AGCT 4 cut(s) 188, 254, 413, 520
AluI AGCT 4 cut(s) 188, 254, 413, 520
Alw26I GTCTC 1 cut(s) 431
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 1 cut(s) 41
ApeKI GCWGC 2 cut(s) 4, 632
ApoI RAATTY 5 cut(s) 11, 175, 332, 447, 476
AsuHPI GGTGA 3 cut(s) 157, 341, 587
BanII GRGCYC 1 cut(s) 552
BbsI GAAGAC 2 cut(s) 270, 381
BbvI GCAGC 2 cut(s) 16, 619
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 1 cut(s) 185
BisI GCNGC 3 cut(s) 5, 356, 633
BlsI GCNGC 3 cut(s) 6, 357, 634
BmgBI CACGTC 1 cut(s) 428
BmiI GGNNCC 3 cut(s) 549, 654, 669
BpiI GAAGAC 2 cut(s) 270, 381
BpuEI CTTGAG 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 672
Bsc4I CCNNNNNNNGG 2 cut(s) 466, 491
Bse118I RCCGGY 1 cut(s) 351
Bse1I ACTGG 2 cut(s) 136, 301
BseDI CCNNGG 1 cut(s) 672
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 2 cut(s) 466, 491
BseMII CTCAG 2 cut(s) 218, 426
BseNI ACTGG 2 cut(s) 136, 301
BseRI GAGGAG 4 cut(s) 109, 112, 393, 665
BseXI GCAGC 2 cut(s) 16, 619
BseYI CCCAGC 1 cut(s) 383
BshFI GGCC 1 cut(s) 43
BsiSI CCGG 1 cut(s) 352
BslFI GGGAC 1 cut(s) 581
BslI CCNNNNNNNGG 2 cut(s) 466, 491
BsmAI GTCTC 1 cut(s) 431
BsmFI GGGAC 1 cut(s) 581
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 552
Bsp143I GATC 1 cut(s) 532
BspACI CCGC 1 cut(s) 355
BspANI GGCC 1 cut(s) 43
BspCNI CTCAG 2 cut(s) 219, 427
BspLI GGNNCC 3 cut(s) 549, 654, 669
BsrFI RCCGGY 1 cut(s) 351
BsrI ACTGG 2 cut(s) 136, 301
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 672
BssMI GATC 1 cut(s) 532
BssT1I CCWWGG 1 cut(s) 672
Bst4CI ACNGT 1 cut(s) 115
Bst6I CTCTTC 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 380
BstDEI CTNAG 2 cut(s) 227, 435
BstF5I GGATG 1 cut(s) 424
BstKTI GATC 1 cut(s) 535
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 1 cut(s) 532
BstMWI GCNNNNNNNGC 3 cut(s) 380, 440, 629
BstV1I GCAGC 2 cut(s) 16, 619
BstV2I GAAGAC 2 cut(s) 270, 381
BsuRI GGCC 1 cut(s) 43
BtrI CACGTC 1 cut(s) 428
BtsCI GGATG 1 cut(s) 424
CaiI CAGNNNCTG 1 cut(s) 569
Cfr10I RCCGGY 1 cut(s) 351
CviAII CATG 1 cut(s) 502
CviJI RGCY 9 cut(s) 7, 43, 188, 254, 413, 434, 520, 550, 655
CviKI_1 RGCY 9 cut(s) 7, 43, 188, 254, 413, 434, 520, 550, 655
DdeI CTNAG 2 cut(s) 227, 435
DpnI GATC 1 cut(s) 534
DpnII GATC 1 cut(s) 532
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 672
Eco24I GRGCYC 1 cut(s) 552
Eco57I CTGAAG 1 cut(s) 591
EcoT14I CCWWGG 1 cut(s) 672
EcoT38I GRGCYC 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 672
FaeI CATG 1 cut(s) 505
FaiI YATR 9 cut(s) 18, 165, 201, 203, 503, 557, 626, 648, 658
FaqI GGGAC 1 cut(s) 581
FatI CATG 1 cut(s) 501
Fnu4HI GCNGC 3 cut(s) 5, 356, 633
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 3 cut(s) 5, 356, 633
FspBI CTAG 1 cut(s) 185
GluI GCNGC 3 cut(s) 5, 356, 633
GsaI CCCAGC 1 cut(s) 387
HaeIII GGCC 1 cut(s) 43
HapII CCGG 1 cut(s) 352
Hin1II CATG 1 cut(s) 505
HinfI GANTC 1 cut(s) 231
HpaII CCGG 1 cut(s) 352
HphI GGTGA 3 cut(s) 157, 341, 587
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 2 cut(s) 236, 456
Hpy188III TCNNGA 5 cut(s) 407, 416, 464, 536, 679
Hpy8I GTNNAC 1 cut(s) 216
HpyAV CCTTC 2 cut(s) 42, 566
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 2 cut(s) 4, 374
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 440, 629
HpyF3I CTNAG 2 cut(s) 227, 435
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 1 cut(s) 505
Kzo9I GATC 1 cut(s) 532
LmnI GCTCC 4 cut(s) 246, 487, 547, 652
LpnPI CCDG 8 cut(s) 117, 248, 314, 365, 369, 392, 401, 416
Lsp1109I GCAGC 2 cut(s) 16, 619
MaeI CTAG 1 cut(s) 185
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 1 cut(s) 288
MalI GATC 1 cut(s) 534
MboI GATC 1 cut(s) 532
MboII GAAGA 9 cut(s) 49, 160, 270, 338, 381, 384, 387, 390, 485
MhlI GDGCHC 1 cut(s) 552
MluCI AATT 9 cut(s) 11, 175, 332, 447, 476, 489, 580, 627, 643
MnlI CCTC 8 cut(s) 87, 90, 265, 414, 460, 471, 562, 643
MseI TTAA 2 cut(s) 90, 318
MspI CCGG 1 cut(s) 352
Mva1269I GAATGC 1 cut(s) 625
MwoI GCNNNNNNNGC 3 cut(s) 380, 440, 629
NdeII GATC 1 cut(s) 532
NlaIII CATG 1 cut(s) 505
NlaIV GGNNCC 3 cut(s) 549, 654, 669
PctI GAATGC 1 cut(s) 625
PfeI GAWTC 1 cut(s) 231
PkrI GCNGC 3 cut(s) 6, 357, 634
PspFI CCCAGC 1 cut(s) 383
PspN4I GGNNCC 3 cut(s) 549, 654, 669
PstNI CAGNNNCTG 1 cut(s) 569
SaqAI TTAA 2 cut(s) 90, 318
SatI GCNGC 3 cut(s) 5, 356, 633
Sau3AI GATC 1 cut(s) 532
SduI GDGCHC 1 cut(s) 552
SgrAI CRCCGGYG 1 cut(s) 351
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
Sse9I AATT 9 cut(s) 11, 175, 332, 447, 476, 489, 580, 627, 643
SsiI CCGC 1 cut(s) 355
SspI AATATT 1 cut(s) 87
SspMI CTAG 1 cut(s) 185
StyI CCWWGG 1 cut(s) 672
TaaI ACNGT 1 cut(s) 115
TaiI ACGT 1 cut(s) 430
TaqI TCGA 1 cut(s) 445
TasI AATT 9 cut(s) 11, 175, 332, 447, 476, 489, 580, 627, 643
TauI GCSGC 1 cut(s) 358
TfiI GAWTC 1 cut(s) 231
Tru1I TTAA 2 cut(s) 90, 318
Tru9I TTAA 2 cut(s) 90, 318
TseI GCWGC 2 cut(s) 4, 632
TspDTI ATGAA 1 cut(s) 111
TspGWI ACGGA 1 cut(s) 575
XapI RAATTY 5 cut(s) 11, 175, 332, 447, 476
XspI CTAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.