Rh6DG466400

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0015435
Physical Location & Seq
Forward (+)
63387160 .. 63388278
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG466400.1

Sequence Viewer

Length: 441 bp
ATGGGGAGCAGGGCTTTGCTTCTGGTGAAGGAGGTGTCTTCTTCTTTGTTACCCAAGAACCAGTTTTTACCCAACATTAACTCCCACCGAAAATTTCCGCAAGTGCTTCACCGGCGGCGAAGTTTTTCAGCTGCAACAACTGCTGGGTCTTCTCTGGAACCTCTGGATGTGCCACGTCTGGCTGAGACTGCTCGAATTTCTCTGACCCTTCAAGAGGTTGAAGAATTTGCTCCCAAAATTGGACAAGTCATGGAATGGTTTGGACAGCTTCAAGCAGTTGATCTTGAAAGCGTGGAGCCCTCTATAAGAGCAGATACTGAAGGTGATAATTTCCGTGCTGATGTCCCCGAAACATTTGAGAATAGGAATGCCATAATTGCTGCTATTCCAAATTATGAGGAGCCATACATCAAGGTTCCCAAGGTCTTGAACAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
Glu-tRNAGln

Protein Analysis

146

Amino Acids

16.37

Weight (kDa)

5.51

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GatC PF02686 69 - 139 6.8e-13 Glu-tRNAGln amidotransferase C subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32915
fragaria_vesca FvH4_2g35131
malus_domestica MD08G1108800.v1.1
prunus_persica Prupe.1G443400_v2.0.a1 Prupe.1G443400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306821
rosa_laevigata RLG00000010763
rosa_multiflora Rmu_sc0016250.1_g000001 Rmu_sc0027973.1_g000001
rosa_roxburghii Rroxscaffold_7G00161440
rosa_rugosa Rorug06G0353400
rosa_samantha Rh6AG465100 Rh6BG435700 Rh6CG479500 Rh6DG466400
rosa_wichuraiana Rw6G040530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 98, 115
AcsI RAATTY 3 cut(s) 92, 195, 224
AcuI CTGAAG 1 cut(s) 339
AfiI CCNNNNNNNGG 2 cut(s) 214, 239
AgsI TTSAA 5 cut(s) 212, 221, 272, 287, 430
AjiI CACGTC 1 cut(s) 176
AluBI AGCT 2 cut(s) 131, 268
AluI AGCT 2 cut(s) 131, 268
Alw26I GTCTC 1 cut(s) 179
AlwNI CAGNNNCTG 1 cut(s) 317
ApeKI GCWGC 2 cut(s) 131, 380
ApoI RAATTY 3 cut(s) 92, 195, 224
Asp700I GAANNNNTTC 1 cut(s) 124
AsuHPI GGTGA 3 cut(s) 37, 101, 335
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 2 cut(s) 30, 141
BbvI GCAGC 2 cut(s) 118, 367
BcoDI GTCTC 1 cut(s) 179
BisI GCNGC 3 cut(s) 116, 132, 381
BlsI GCNGC 3 cut(s) 117, 133, 382
BmgBI CACGTC 1 cut(s) 176
BmiI GGNNCC 4 cut(s) 159, 297, 402, 417
BpiI GAAGAC 2 cut(s) 30, 141
BsaJI CCNNGG 1 cut(s) 420
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 239
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 420
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 2 cut(s) 214, 239
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 2 cut(s) 118, 367
BseYI CCCAGC 1 cut(s) 143
BsiSI CCGG 1 cut(s) 112
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 2 cut(s) 214, 239
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 1 cut(s) 280
BspACI CCGC 2 cut(s) 98, 115
BspCNI CTCAG 1 cut(s) 175
BspLI GGNNCC 4 cut(s) 159, 297, 402, 417
BsrFI RCCGGY 1 cut(s) 111
BsrI ACTGG 1 cut(s) 61
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 420
BssMI GATC 1 cut(s) 280
BssT1I CCWWGG 1 cut(s) 420
BstAPI GCANNNNNTGC 1 cut(s) 140
BstDEI CTNAG 1 cut(s) 183
BstENI CCTNNNNNAGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 172
BstKTI GATC 1 cut(s) 283
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 112, 140, 188, 377
BstV1I GCAGC 2 cut(s) 118, 367
BstV2I GAAGAC 2 cut(s) 30, 141
BtrI CACGTC 1 cut(s) 176
BtsCI GGATG 1 cut(s) 172
CaiI CAGNNNCTG 1 cut(s) 317
Cfr10I RCCGGY 1 cut(s) 111
CviAII CATG 1 cut(s) 250
CviJI RGCY 6 cut(s) 14, 131, 182, 268, 298, 403
CviKI_1 RGCY 6 cut(s) 14, 131, 182, 268, 298, 403
DdeI CTNAG 1 cut(s) 183
DpnI GATC 1 cut(s) 282
DpnII GATC 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 300
Eco57I CTGAAG 1 cut(s) 339
EcoNI CCTNNNNNAGG 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 1 cut(s) 253
FaiI YATR 5 cut(s) 251, 305, 374, 396, 406
FaqI GGGAC 1 cut(s) 329
FatI CATG 1 cut(s) 249
Fnu4HI GCNGC 3 cut(s) 116, 132, 381
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 3 cut(s) 116, 132, 381
GluI GCNGC 3 cut(s) 116, 132, 381
GsaI CCCAGC 1 cut(s) 147
HapII CCGG 1 cut(s) 112
Hin1II CATG 1 cut(s) 253
HpaII CCGG 1 cut(s) 112
HphI GGTGA 3 cut(s) 37, 101, 335
Hpy188I TCNGA 1 cut(s) 204
Hpy188III TCNNGA 5 cut(s) 155, 164, 212, 284, 427
HpyAV CCTTC 3 cut(s) 22, 218, 314
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 140, 188, 377
HpyF3I CTNAG 1 cut(s) 183
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 1 cut(s) 253
Kzo9I GATC 1 cut(s) 280
LmnI GCTCC 4 cut(s) 6, 235, 295, 400
LpnPI CCDG 7 cut(s) 8, 74, 125, 129, 140, 149, 164
Lsp1109I GCAGC 2 cut(s) 118, 367
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 48
MalI GATC 1 cut(s) 282
MboI GATC 1 cut(s) 280
MboII GAAGA 4 cut(s) 30, 33, 141, 233
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 7 cut(s) 92, 195, 224, 237, 328, 375, 391
MnlI CCTC 5 cut(s) 25, 171, 208, 310, 391
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 373
MwoI GCNNNNNNNGC 4 cut(s) 112, 140, 188, 377
NdeII GATC 1 cut(s) 280
NlaIII CATG 1 cut(s) 253
NlaIV GGNNCC 4 cut(s) 159, 297, 402, 417
PctI GAATGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 124
PkrI GCNGC 3 cut(s) 117, 133, 382
PspFI CCCAGC 1 cut(s) 143
PspN4I GGNNCC 4 cut(s) 159, 297, 402, 417
PstNI CAGNNNCTG 1 cut(s) 317
PvuII CAGCTG 1 cut(s) 131
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 3 cut(s) 116, 132, 381
Sau3AI GATC 1 cut(s) 280
SduI GDGCHC 1 cut(s) 300
SetI ASST 9 cut(s) 36, 133, 163, 178, 219, 270, 325, 417, 426
SgrAI CRCCGGYG 1 cut(s) 111
Sse9I AATT 7 cut(s) 92, 195, 224, 237, 328, 375, 391
SsiI CCGC 2 cut(s) 98, 115
StyI CCWWGG 1 cut(s) 420
TaiI ACGT 1 cut(s) 178
TaqI TCGA 1 cut(s) 193
TasI AATT 7 cut(s) 92, 195, 224, 237, 328, 375, 391
TauI GCSGC 1 cut(s) 118
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseI GCWGC 2 cut(s) 131, 380
TspGWI ACGGA 1 cut(s) 323
XagI CCTNNNNNAGG 1 cut(s) 212
XapI RAATTY 3 cut(s) 92, 195, 224
XmnI GAANNNNTTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.