Rw6G040530

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015435
Physical Location & Seq
Forward (+)
64298378 .. 64299330
953 bp
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UTR
Exon/CDS
Intron
Rw6G040530.1

Sequence Viewer

Length: 444 bp
ATGGGGAGCAGAGCTTTGCTTCTGGTGAAGGAGGTGTCTTCTTCTTTGTTACCCAAGAACCAGTTTTTACCCAACTTTAACTCCCACCGAAAATTTCCGAAAGTGCTTCACCGGCGGCGAAGTTTTTCGGCTGCAACAACTGCTGGGTCTCCTCCTCTGGAAGCTCTGGATGTGCCACGTCTGGCTGAGACTGCTCGAATTTCTCTGACCCCTCAAGAGGTTGAAGAATTTGCTCCCAAAATTGGACAAGTCATGGAATGGTTTGGACAGCTTCAAGCAGTTGATCTTGAAAGCGTGGAGCCCTCTATAAGAGCAGGTGCTTCAGAACCCCTATCATACATGCTGATATGGAATTTGTTGTCCAACAGGAATGCCATAATTGCTGCTATTCCAAATTATGAGGAGCCATACATCAAGGTTCCCAAGGTCTTGAACAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
Glu-tRNAGln

Protein Analysis

147

Amino Acids

16.42

Weight (kDa)

9.0

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GatC PF02686 69 - 140 5.1e-09 Glu-tRNAGln amidotransferase C subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32915
fragaria_vesca FvH4_2g35131
malus_domestica MD08G1108800.v1.1
prunus_persica Prupe.1G443400_v2.0.a1 Prupe.1G443400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306821
rosa_laevigata RLG00000010763
rosa_multiflora Rmu_sc0016250.1_g000001 Rmu_sc0027973.1_g000001
rosa_roxburghii Rroxscaffold_7G00161440
rosa_rugosa Rorug06G0353400
rosa_samantha Rh6AG465100 Rh6BG435700 Rh6CG479500 Rh6DG466400
rosa_wichuraiana Rw6G040530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 305
Acc36I ACCTGC 1 cut(s) 305
AciI CCGC 1 cut(s) 115
AcsI RAATTY 4 cut(s) 92, 198, 227, 352
AcuI CTGAAG 1 cut(s) 306
AfiI CCNNNNNNNGG 2 cut(s) 217, 242
AgsI TTSAA 4 cut(s) 224, 275, 290, 433
AjiI CACGTC 1 cut(s) 179
AluBI AGCT 3 cut(s) 14, 164, 271
AluI AGCT 3 cut(s) 14, 164, 271
Alw26I GTCTC 2 cut(s) 153, 182
ApeKI GCWGC 2 cut(s) 131, 383
ApoI RAATTY 4 cut(s) 92, 198, 227, 352
Asp700I GAANNNNTTC 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 37, 101
BanII GRGCYC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 118, 370
BcoDI GTCTC 2 cut(s) 153, 182
BfuAI ACCTGC 1 cut(s) 305
BisI GCNGC 3 cut(s) 116, 132, 384
BlsI GCNGC 3 cut(s) 117, 133, 385
BmgBI CACGTC 1 cut(s) 179
BmiI GGNNCC 3 cut(s) 300, 405, 420
BpiI GAAGAC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 423
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 242
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 217, 242
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 3 cut(s) 141, 144, 416
BseXI GCAGC 2 cut(s) 118, 370
BseYI CCCAGC 1 cut(s) 143
BsiSI CCGG 1 cut(s) 112
BslI CCNNNNNNNGG 2 cut(s) 217, 242
BsmAI GTCTC 2 cut(s) 153, 182
BsmI GAATGC 1 cut(s) 376
Bso31I GGTCTC 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 1 cut(s) 115
BspCNI CTCAG 1 cut(s) 178
BspLI GGNNCC 3 cut(s) 300, 405, 420
BspMI ACCTGC 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 111
BsrI ACTGG 1 cut(s) 61
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 1 cut(s) 283
BssT1I CCWWGG 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 140
BstDEI CTNAG 1 cut(s) 186
BstF5I GGATG 1 cut(s) 175
BstKTI GATC 1 cut(s) 286
BstMAI GTCTC 2 cut(s) 153, 182
BstMBI GATC 1 cut(s) 283
BstMWI GCNNNNNNNGC 4 cut(s) 112, 140, 191, 380
BstNSI RCATGY 1 cut(s) 343
BstV1I GCAGC 2 cut(s) 118, 370
BstV2I GAAGAC 1 cut(s) 30
BtrI CACGTC 1 cut(s) 179
BtsCI GGATG 1 cut(s) 175
BveI ACCTGC 1 cut(s) 305
Cfr10I RCCGGY 1 cut(s) 111
CviAII CATG 2 cut(s) 253, 340
CviJI RGCY 7 cut(s) 14, 131, 164, 185, 271, 301, 406
CviKI_1 RGCY 7 cut(s) 14, 131, 164, 185, 271, 301, 406
DdeI CTNAG 1 cut(s) 186
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 2 cut(s) 256, 343
FaiI YATR 8 cut(s) 254, 308, 337, 341, 349, 377, 399, 409
FatI CATG 2 cut(s) 252, 339
Fnu4HI GCNGC 3 cut(s) 116, 132, 384
FokI GGATG 1 cut(s) 182
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 3 cut(s) 116, 132, 384
GluI GCNGC 3 cut(s) 116, 132, 384
GsaI CCCAGC 1 cut(s) 147
HapII CCGG 1 cut(s) 112
Hin1II CATG 2 cut(s) 256, 343
HpaII CCGG 1 cut(s) 112
HphI GGTGA 2 cut(s) 37, 101
Hpy188I TCNGA 3 cut(s) 99, 207, 325
Hpy188III TCNNGA 5 cut(s) 158, 167, 215, 287, 430
HpyAV CCTTC 1 cut(s) 22
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 140, 191, 380
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 2 cut(s) 256, 343
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 4 cut(s) 6, 238, 298, 403
LpnPI CCDG 9 cut(s) 8, 74, 125, 129, 143, 152, 167, 300, 352
Lsp1109I GCAGC 2 cut(s) 118, 370
MaeII ACGT 1 cut(s) 178
MaeIII GTNAC 1 cut(s) 48
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 3 cut(s) 30, 33, 236
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 7 cut(s) 92, 198, 227, 240, 352, 378, 394
MmeI TCCRAC 1 cut(s) 387
MnlI CCTC 7 cut(s) 25, 162, 165, 211, 222, 313, 394
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 1 cut(s) 78
MspI CCGG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 376
MwoI GCNNNNNNNGC 4 cut(s) 112, 140, 191, 380
NdeII GATC 1 cut(s) 283
NlaIII CATG 2 cut(s) 256, 343
NlaIV GGNNCC 3 cut(s) 300, 405, 420
NspI RCATGY 1 cut(s) 343
PaqCI CACCTGC 1 cut(s) 305
PctI GAATGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 124
PkrI GCNGC 3 cut(s) 117, 133, 385
PspFI CCCAGC 1 cut(s) 143
PspN4I GGNNCC 3 cut(s) 300, 405, 420
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 3 cut(s) 116, 132, 384
Sau3AI GATC 1 cut(s) 283
SduI GDGCHC 1 cut(s) 303
SetI ASST 9 cut(s) 16, 36, 166, 181, 222, 273, 319, 420, 429
SgrAI CRCCGGYG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 213
SmoI CTYRAG 1 cut(s) 213
Sse9I AATT 7 cut(s) 92, 198, 227, 240, 352, 378, 394
SsiI CCGC 1 cut(s) 115
StyI CCWWGG 1 cut(s) 423
TaiI ACGT 1 cut(s) 181
TaqI TCGA 1 cut(s) 196
TasI AATT 7 cut(s) 92, 198, 227, 240, 352, 378, 394
TauI GCSGC 1 cut(s) 118
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseI GCWGC 2 cut(s) 131, 383
XapI RAATTY 4 cut(s) 92, 198, 227, 352
XceI RCATGY 1 cut(s) 343
XmnI GAANNNNTTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.