MD08G1108800.v1.1

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015435
Physical Location & Seq
Reverse (-)
9578277 .. 9580291
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1108800.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGGAAGCAGAGCTTTGCTTCTACTGAAGGGATTATCTTGTTCCTCGCTACCCAAGAAGCTGCTTTTCCCAAAAGTCCTTCCTCCTCGTAACATCAATTGGGTTCGAACAAATTGCTCGACACCCAAAAGCAGCGGCCTTCCTTTGGAGCCTCCGGACTTGCCACGCTTGGCCGAGACTGCTCGAATTACTCTGACCCCTCATGAGGTAGAAGAATTTGCTCCCAAAATTGGGCAAGTCATAGATTGGTTTGGACAGCTTCAAGCTGTTGATCTTGAAACTGTGGAGCCCTCAATCAGAGCAGATACTGAAGGCGATAGTTTCCGTGAAGATATTCCTGAAACGTTTGAGAACAGGGAGGCCATGATTGCTGCCGTTCCAAACTATGAGGAGCAGTATATTAAAGTTCCCAAAGTCTTGAACAAGGAGTGTGGAGTGCGGAAAGGCAACCGAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
Glu-tRNAGln

Protein Analysis

153

Amino Acids

17.01

Weight (kDa)

5.93

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GatC PF02686 65 - 136 8.2e-15 Glu-tRNAGln amidotransferase C subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32915
fragaria_vesca FvH4_2g35131
malus_domestica MD08G1108800.v1.1
prunus_persica Prupe.1G443400_v2.0.a1 Prupe.1G443400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306821
rosa_laevigata RLG00000010763
rosa_multiflora Rmu_sc0016250.1_g000001 Rmu_sc0027973.1_g000001
rosa_roxburghii Rroxscaffold_7G00161440
rosa_rugosa Rorug06G0353400
rosa_samantha Rh6AG465100 Rh6BG435700 Rh6CG479500 Rh6DG466400
rosa_wichuraiana Rw6G040530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 154
AciI CCGC 2 cut(s) 135, 439
AclI AACGTT 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 215
AcuI CTGAAG 2 cut(s) 47, 330
AfiI CCNNNNNNNGG 4 cut(s) 145, 205, 230, 231
AgsI TTSAA 3 cut(s) 263, 278, 421
AluBI AGCT 4 cut(s) 14, 61, 259, 266
AluI AGCT 4 cut(s) 14, 61, 259, 266
Alw26I GTCTC 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 308
Aor13HI TCCGGA 1 cut(s) 154
AoxI GGCC 3 cut(s) 136, 171, 360
ApeKI GCWGC 3 cut(s) 61, 132, 371
ApoI RAATTY 1 cut(s) 215
Asp700I GAANNNNTTC 1 cut(s) 333
AsuII TTCGAA 1 cut(s) 106
BanII GRGCYC 1 cut(s) 291
BbvI GCAGC 3 cut(s) 48, 144, 358
BceAI ACGGC 1 cut(s) 359
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BcoDI GTCTC 1 cut(s) 170
BisI GCNGC 4 cut(s) 62, 133, 136, 372
BlsI GCNGC 4 cut(s) 63, 134, 137, 373
BmiI GGNNCC 2 cut(s) 150, 288
Bpu14I TTCGAA 1 cut(s) 106
BsaWI WCCGGW 1 cut(s) 154
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 205, 230, 231
BseAI TCCGGA 1 cut(s) 154
BseLI CCNNNNNNNGG 4 cut(s) 145, 205, 230, 231
BseRI GAGGAG 2 cut(s) 75, 404
BseXI GCAGC 3 cut(s) 48, 144, 358
BshFI GGCC 3 cut(s) 138, 173, 362
BsiSI CCGG 1 cut(s) 155
BslI CCNNNNNNNGG 4 cut(s) 145, 205, 230, 231
BsmAI GTCTC 1 cut(s) 170
BsnI GGCC 3 cut(s) 138, 173, 362
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 1 cut(s) 291
Bsp13I TCCGGA 1 cut(s) 154
Bsp143I GATC 1 cut(s) 271
BspACI CCGC 2 cut(s) 135, 439
BspANI GGCC 3 cut(s) 138, 173, 362
BspEI TCCGGA 1 cut(s) 154
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 2 cut(s) 150, 288
BspT104I TTCGAA 1 cut(s) 106
BssMI GATC 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 283
BstBI TTCGAA 1 cut(s) 106
BstDEI CTNAG 1 cut(s) 456
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 1 cut(s) 271
BstMWI GCNNNNNNNGC 2 cut(s) 179, 368
BstV1I GCAGC 3 cut(s) 48, 144, 358
BsuRI GGCC 3 cut(s) 138, 173, 362
CaiI CAGNNNCTG 1 cut(s) 308
CciI TCATGA 1 cut(s) 202
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 2 cut(s) 203, 364
CviJI RGCY 9 cut(s) 14, 61, 138, 151, 173, 259, 266, 289, 362
CviKI_1 RGCY 9 cut(s) 14, 61, 138, 151, 173, 259, 266, 289, 362
DdeI CTNAG 1 cut(s) 456
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 291
Eco57I CTGAAG 2 cut(s) 47, 330
EcoT38I GRGCYC 1 cut(s) 291
FaeI CATG 2 cut(s) 206, 367
FaiI YATR 5 cut(s) 204, 242, 365, 387, 399
FalI AAGNNNNNCTT 1 cut(s) 30
FatI CATG 2 cut(s) 202, 363
Fnu4HI GCNGC 4 cut(s) 62, 133, 136, 372
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 4 cut(s) 62, 133, 136, 372
GluI GCNGC 4 cut(s) 62, 133, 136, 372
HaeIII GGCC 3 cut(s) 138, 173, 362
HapII CCGG 1 cut(s) 155
Hin1II CATG 2 cut(s) 206, 367
HpaII CCGG 1 cut(s) 155
Hpy188I TCNGA 2 cut(s) 195, 299
Hpy188III TCNNGA 5 cut(s) 155, 203, 275, 338, 418
HpyAV CCTTC 4 cut(s) 22, 89, 149, 305
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 368
HpyF3I CTNAG 1 cut(s) 456
HpySE526I ACGT 1 cut(s) 344
Hsp92II CATG 2 cut(s) 206, 367
Kpn2I TCCGGA 1 cut(s) 154
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 4 cut(s) 148, 226, 286, 391
LpnPI CCDG 3 cut(s) 168, 340, 351
Lsp1109I GCAGC 3 cut(s) 48, 144, 358
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 1 cut(s) 89
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 2 cut(s) 224, 341
MfeI CAATTG 1 cut(s) 97
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 5 cut(s) 97, 112, 186, 215, 228
MnlI CCTC 9 cut(s) 55, 93, 96, 162, 199, 210, 301, 352, 382
MroI TCCGGA 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 1 cut(s) 402
MspA1I CMGCKG 1 cut(s) 135
MspI CCGG 1 cut(s) 155
MunI CAATTG 1 cut(s) 97
MwoI GCNNNNNNNGC 2 cut(s) 179, 368
NdeII GATC 1 cut(s) 271
NlaIII CATG 2 cut(s) 206, 367
NlaIV GGNNCC 2 cut(s) 150, 288
NmeAIII GCCGAG 1 cut(s) 199
NspV TTCGAA 1 cut(s) 106
PagI TCATGA 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 333
PkrI GCNGC 4 cut(s) 63, 134, 137, 373
Psp1406I AACGTT 1 cut(s) 344
PspN4I GGNNCC 2 cut(s) 150, 288
PstNI CAGNNNCTG 1 cut(s) 308
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 4 cut(s) 62, 133, 136, 372
Sau3AI GATC 1 cut(s) 271
SduI GDGCHC 1 cut(s) 291
SetI ASST 6 cut(s) 16, 63, 210, 261, 268, 347
SfuI TTCGAA 1 cut(s) 106
Sse9I AATT 5 cut(s) 97, 112, 186, 215, 228
SsiI CCGC 2 cut(s) 135, 439
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 1 cut(s) 347
TaqI TCGA 3 cut(s) 106, 119, 184
TasI AATT 5 cut(s) 97, 112, 186, 215, 228
TauI GCSGC 1 cut(s) 138
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TseI GCWGC 3 cut(s) 61, 132, 371
TspGWI ACGGA 1 cut(s) 314
XapI RAATTY 1 cut(s) 215
XmnI GAANNNNTTC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.