Rh6AG465100

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0015435
Physical Location & Seq
Forward (+)
64719299 .. 64720437
1139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG465100.1

Sequence Viewer

Length: 444 bp
ATGGGGAGCAGAGCTTTGCTTCTGGTGAAGGAGGTGTCTTCTTCTTTGTTACCCAAGAACCAGTTTTTACCCAACTTTAACTCCCACCGAAAATTTGCGCAAGTGCTTCACCGGCGGCGAAGTTTTTCAGCTGCAACAACTGCTGGGTCTCCTCCTCTGGAAGCTCTGGATGTGCCACGTCTGGCTGAGACTGCTCGAATTTCTCTGACCCCTCAAGAGGTTGAAGAATTTGCTCCCAAAATTGGACAAGTCATGGAATGGTTTGGACAGCTTCAAGCAGTTGATCTTGAAAGCGTGGAGCCCTCTATAAGAGCAGATACTGAAGGTGATAATTTCCGTGCTGATGTCCCCGAAACATTTGAGAATAGGAATGCCATAATTGCTGCTATTCCAAATTATGAGGAGCCATACATCAAGGTTCCCAAGGTCTTGAACAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
Glu-tRNAGln

Protein Analysis

147

Amino Acids

16.45

Weight (kDa)

5.51

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GatC PF02686 69 - 140 7.9e-14 Glu-tRNAGln amidotransferase C subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32915
fragaria_vesca FvH4_2g35131
malus_domestica MD08G1108800.v1.1
prunus_persica Prupe.1G443400_v2.0.a1 Prupe.1G443400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306821
rosa_laevigata RLG00000010763
rosa_multiflora Rmu_sc0016250.1_g000001 Rmu_sc0027973.1_g000001
rosa_roxburghii Rroxscaffold_7G00161440
rosa_rugosa Rorug06G0353400
rosa_samantha Rh6AG465100 Rh6BG435700 Rh6CG479500 Rh6DG466400
rosa_wichuraiana Rw6G040530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 99
AciI CCGC 1 cut(s) 115
AcsI RAATTY 3 cut(s) 92, 198, 227
AcuI CTGAAG 1 cut(s) 342
AfiI CCNNNNNNNGG 2 cut(s) 217, 242
AgsI TTSAA 4 cut(s) 224, 275, 290, 433
AjiI CACGTC 1 cut(s) 179
AluBI AGCT 4 cut(s) 14, 131, 164, 271
AluI AGCT 4 cut(s) 14, 131, 164, 271
Alw26I GTCTC 2 cut(s) 153, 182
AlwNI CAGNNNCTG 1 cut(s) 320
ApeKI GCWGC 2 cut(s) 131, 383
ApoI RAATTY 3 cut(s) 92, 198, 227
Asp700I GAANNNNTTC 1 cut(s) 124
AspLEI GCGC 1 cut(s) 100
AsuHPI GGTGA 3 cut(s) 37, 101, 338
BanII GRGCYC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 118, 370
BcoDI GTCTC 2 cut(s) 153, 182
BisI GCNGC 3 cut(s) 116, 132, 384
BlsI GCNGC 3 cut(s) 117, 133, 385
BmgBI CACGTC 1 cut(s) 179
BmiI GGNNCC 3 cut(s) 300, 405, 420
BpiI GAAGAC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 423
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 242
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 217, 242
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 3 cut(s) 141, 144, 416
BseXI GCAGC 2 cut(s) 118, 370
BseYI CCCAGC 1 cut(s) 143
BsiSI CCGG 1 cut(s) 112
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 217, 242
BsmAI GTCTC 2 cut(s) 153, 182
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 1 cut(s) 376
Bso31I GGTCTC 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 1 cut(s) 115
BspCNI CTCAG 1 cut(s) 178
BspLI GGNNCC 3 cut(s) 300, 405, 420
BspTNI GGTCTC 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 111
BsrI ACTGG 1 cut(s) 61
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 1 cut(s) 283
BssT1I CCWWGG 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 140
BstDEI CTNAG 1 cut(s) 186
BstF5I GGATG 1 cut(s) 175
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 1 cut(s) 286
BstMAI GTCTC 2 cut(s) 153, 182
BstMBI GATC 1 cut(s) 283
BstMWI GCNNNNNNNGC 4 cut(s) 112, 140, 191, 380
BstV1I GCAGC 2 cut(s) 118, 370
BstV2I GAAGAC 1 cut(s) 30
BtrI CACGTC 1 cut(s) 179
BtsCI GGATG 1 cut(s) 175
CaiI CAGNNNCTG 1 cut(s) 320
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 1 cut(s) 111
CviAII CATG 1 cut(s) 253
CviJI RGCY 7 cut(s) 14, 131, 164, 185, 271, 301, 406
CviKI_1 RGCY 7 cut(s) 14, 131, 164, 185, 271, 301, 406
DdeI CTNAG 1 cut(s) 186
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 1 cut(s) 256
FaiI YATR 5 cut(s) 254, 308, 377, 399, 409
FaqI GGGAC 1 cut(s) 332
FatI CATG 1 cut(s) 252
Fnu4HI GCNGC 3 cut(s) 116, 132, 384
FokI GGATG 1 cut(s) 182
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 3 cut(s) 116, 132, 384
FspI TGCGCA 1 cut(s) 99
GlaI GCGC 1 cut(s) 99
GluI GCNGC 3 cut(s) 116, 132, 384
GsaI CCCAGC 1 cut(s) 147
HapII CCGG 1 cut(s) 112
HhaI GCGC 1 cut(s) 100
Hin1II CATG 1 cut(s) 256
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HpaII CCGG 1 cut(s) 112
HphI GGTGA 3 cut(s) 37, 101, 338
Hpy188I TCNGA 1 cut(s) 207
Hpy188III TCNNGA 5 cut(s) 158, 167, 215, 287, 430
HpyAV CCTTC 2 cut(s) 22, 317
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 140, 191, 380
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 1 cut(s) 256
HspAI GCGC 1 cut(s) 98
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 4 cut(s) 6, 238, 298, 403
LpnPI CCDG 7 cut(s) 8, 74, 125, 129, 143, 152, 167
Lsp1109I GCAGC 2 cut(s) 118, 370
MaeII ACGT 1 cut(s) 178
MaeIII GTNAC 1 cut(s) 48
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 3 cut(s) 30, 33, 236
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 7 cut(s) 92, 198, 227, 240, 331, 378, 394
MnlI CCTC 7 cut(s) 25, 162, 165, 211, 222, 313, 394
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 376
MwoI GCNNNNNNNGC 4 cut(s) 112, 140, 191, 380
NdeII GATC 1 cut(s) 283
NlaIII CATG 1 cut(s) 256
NlaIV GGNNCC 3 cut(s) 300, 405, 420
NsbI TGCGCA 1 cut(s) 99
PctI GAATGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 124
PkrI GCNGC 3 cut(s) 117, 133, 385
PspFI CCCAGC 1 cut(s) 143
PspN4I GGNNCC 3 cut(s) 300, 405, 420
PstNI CAGNNNCTG 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 131
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 3 cut(s) 116, 132, 384
Sau3AI GATC 1 cut(s) 283
SduI GDGCHC 1 cut(s) 303
SgrAI CRCCGGYG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 213
SmoI CTYRAG 1 cut(s) 213
Sse9I AATT 7 cut(s) 92, 198, 227, 240, 331, 378, 394
SsiI CCGC 1 cut(s) 115
StyI CCWWGG 1 cut(s) 423
TaiI ACGT 1 cut(s) 181
TaqI TCGA 1 cut(s) 196
TasI AATT 7 cut(s) 92, 198, 227, 240, 331, 378, 394
TauI GCSGC 1 cut(s) 118
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseI GCWGC 2 cut(s) 131, 383
TspGWI ACGGA 1 cut(s) 326
XapI RAATTY 3 cut(s) 92, 198, 227
XmnI GAANNNNTTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.