Rroxscaffold_7G00161440

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0015435
Physical Location & Seq
Reverse (-)
4204343 .. 4205633
1291 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161440.1

Sequence Viewer

Length: 450 bp
ATGGGGAGCAGAGCTTTGCTTCTGGTGAAGGAGGTGTCTTCTTCTTTATTACCCAGGAACCAGTTTTTACCCAACTTTAACTCCCACCGAAAATTTCCGCAAGTGCTTCACCGGCGGCGAAGTTTTTCAACTGCAACAACTGCTGGGTCTTCTCCTCCTCCTCTGGAAGCTCTGGATGTGCCACGTCTGGCTGAGACTGCTCGAATTTCTCTGACCCCGCAAGAGGTTGAAGAATTTGCTCCCAAAATTGGACAAGTCATGGAATGGTTTGGACAGCTTCAAGCAGTTGATCTTGAAAGCGTGGAGCCCTCTATAAGAGCAGATACGGAAGGTGATAATTTCCGGGCTGATGTCCCCGAAACATTTGAGAATAGGAATGCCATAATTGCTGCTATCCCAAATTATGAGGAGCCATACATCAAAGTTCCCAAGGTCTTGAACAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
Glu-tRNAGln

Protein Analysis

149

Amino Acids

16.71

Weight (kDa)

5.51

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GatC PF02686 71 - 142 8.2e-14 Glu-tRNAGln amidotransferase C subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32915
fragaria_vesca FvH4_2g35131
malus_domestica MD08G1108800.v1.1
prunus_persica Prupe.1G443400_v2.0.a1 Prupe.1G443400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306821
rosa_laevigata RLG00000010763
rosa_multiflora Rmu_sc0016250.1_g000001 Rmu_sc0027973.1_g000001
rosa_roxburghii Rroxscaffold_7G00161440
rosa_rugosa Rorug06G0353400
rosa_samantha Rh6AG465100 Rh6BG435700 Rh6CG479500 Rh6DG466400
rosa_wichuraiana Rw6G040530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 98, 115, 218
AcsI RAATTY 3 cut(s) 92, 204, 233
AfiI CCNNNNNNNGG 2 cut(s) 223, 248
AgsI TTSAA 5 cut(s) 129, 230, 281, 296, 439
AjiI CACGTC 1 cut(s) 185
AjnI CCWGG 1 cut(s) 53
AluBI AGCT 3 cut(s) 14, 170, 277
AluI AGCT 3 cut(s) 14, 170, 277
Alw26I GTCTC 1 cut(s) 188
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 3 cut(s) 92, 204, 233
Asp700I GAANNNNTTC 1 cut(s) 124
AsuC2I CCSGG 1 cut(s) 344
AsuHPI GGTGA 3 cut(s) 37, 101, 344
BanII GRGCYC 1 cut(s) 309
BbsI GAAGAC 2 cut(s) 30, 141
BbvI GCAGC 1 cut(s) 376
BciT130I CCWGG 1 cut(s) 55
BcnI CCSGG 1 cut(s) 344
BcoDI GTCTC 1 cut(s) 188
BisI GCNGC 2 cut(s) 116, 390
BlsI GCNGC 2 cut(s) 117, 391
Bme1390I CCNGG 2 cut(s) 55, 344
BmgBI CACGTC 1 cut(s) 185
BmiI GGNNCC 3 cut(s) 59, 306, 411
BmrFI CCNGG 2 cut(s) 55, 344
BpiI GAAGAC 2 cut(s) 30, 141
BpuMI CCSGG 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 53, 429
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 248
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 61
BseBI CCWGG 1 cut(s) 55
BseDI CCNNGG 2 cut(s) 53, 429
BseGI GGATG 1 cut(s) 181
BseLI CCNNNNNNNGG 2 cut(s) 223, 248
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 4 cut(s) 144, 147, 150, 422
BseXI GCAGC 1 cut(s) 376
BseYI CCCAGC 1 cut(s) 143
BsiSI CCGG 2 cut(s) 112, 343
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 2 cut(s) 223, 248
BsmAI GTCTC 1 cut(s) 188
BsmFI GGGAC 1 cut(s) 338
BsmI GAATGC 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 1 cut(s) 289
BspACI CCGC 3 cut(s) 98, 115, 218
BspCNI CTCAG 1 cut(s) 184
BspLI GGNNCC 3 cut(s) 59, 306, 411
BsrFI RCCGGY 1 cut(s) 111
BsrI ACTGG 1 cut(s) 61
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 53, 429
BssMI GATC 1 cut(s) 289
BssT1I CCWWGG 1 cut(s) 429
Bst2UI CCWGG 1 cut(s) 55
BstAPI GCANNNNNTGC 1 cut(s) 140
BstDEI CTNAG 1 cut(s) 192
BstF5I GGATG 1 cut(s) 181
BstKTI GATC 1 cut(s) 292
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 1 cut(s) 289
BstMWI GCNNNNNNNGC 4 cut(s) 112, 140, 197, 386
BstNI CCWGG 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 53, 342
BstV1I GCAGC 1 cut(s) 376
BstV2I GAAGAC 2 cut(s) 30, 141
BtrI CACGTC 1 cut(s) 185
BtsCI GGATG 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 111
CviAII CATG 1 cut(s) 259
CviJI RGCY 7 cut(s) 14, 170, 191, 277, 307, 347, 412
CviKI_1 RGCY 7 cut(s) 14, 170, 191, 277, 307, 347, 412
DdeI CTNAG 1 cut(s) 192
DpnI GATC 1 cut(s) 291
DpnII GATC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 53
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 1 cut(s) 262
FaiI YATR 5 cut(s) 260, 314, 383, 405, 415
FaqI GGGAC 1 cut(s) 338
FatI CATG 1 cut(s) 258
FauI CCCGC 1 cut(s) 225
Fnu4HI GCNGC 2 cut(s) 116, 390
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 2 cut(s) 116, 390
GluI GCNGC 2 cut(s) 116, 390
GsaI CCCAGC 1 cut(s) 147
HapII CCGG 2 cut(s) 112, 343
Hin1II CATG 1 cut(s) 262
HpaII CCGG 2 cut(s) 112, 343
HphI GGTGA 3 cut(s) 37, 101, 344
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 4 cut(s) 164, 173, 293, 436
HpyAV CCTTC 2 cut(s) 22, 323
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 140, 197, 386
HpyF3I CTNAG 1 cut(s) 192
HpySE526I ACGT 1 cut(s) 184
Hsp92II CATG 1 cut(s) 262
Kzo9I GATC 1 cut(s) 289
LmnI GCTCC 4 cut(s) 6, 244, 304, 409
Lsp1109I GCAGC 1 cut(s) 376
MaeII ACGT 1 cut(s) 184
MalI GATC 1 cut(s) 291
MboI GATC 1 cut(s) 289
MboII GAAGA 4 cut(s) 30, 33, 141, 242
MhlI GDGCHC 1 cut(s) 309
MluCI AATT 7 cut(s) 92, 204, 233, 246, 337, 384, 400
MnlI CCTC 7 cut(s) 25, 165, 168, 171, 217, 319, 400
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 1 cut(s) 78
MspI CCGG 2 cut(s) 112, 343
MspR9I CCNGG 2 cut(s) 55, 344
Mva1269I GAATGC 1 cut(s) 382
MvaI CCWGG 1 cut(s) 55
MwoI GCNNNNNNNGC 4 cut(s) 112, 140, 197, 386
NciI CCSGG 1 cut(s) 344
NdeII GATC 1 cut(s) 289
NlaIII CATG 1 cut(s) 262
NlaIV GGNNCC 3 cut(s) 59, 306, 411
PctI GAATGC 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 124
PkrI GCNGC 2 cut(s) 117, 391
Psp6I CCWGG 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 1 cut(s) 53
PspN4I GGNNCC 3 cut(s) 59, 306, 411
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 2 cut(s) 116, 390
Sau3AI GATC 1 cut(s) 289
ScrFI CCNGG 2 cut(s) 55, 344
SduI GDGCHC 1 cut(s) 309
SetI ASST 8 cut(s) 16, 36, 172, 187, 228, 279, 334, 435
SgrAI CRCCGGYG 1 cut(s) 111
Sse9I AATT 7 cut(s) 92, 204, 233, 246, 337, 384, 400
SsiI CCGC 3 cut(s) 98, 115, 218
StyD4I CCNGG 2 cut(s) 53, 342
StyI CCWWGG 1 cut(s) 429
TaiI ACGT 1 cut(s) 187
TaqI TCGA 1 cut(s) 202
TasI AATT 7 cut(s) 92, 204, 233, 246, 337, 384, 400
TauI GCSGC 1 cut(s) 118
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseI GCWGC 1 cut(s) 389
TspGWI ACGGA 1 cut(s) 341
XapI RAATTY 3 cut(s) 92, 204, 233
XmnI GAANNNNTTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.