AT4G33020

transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004683
Physical Location & Seq
Reverse (-)
15932420 .. 15934762
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33020.1

Sequence Viewer

Length: 1035 bp
ATGGCGTCGATCCTTATCTCCGGAGCTGCCGGAGTCTCGATTCCTCTAGTTGGAACCCTACTTCCGTTAAACGGTGGCTTAATGAGGGGAGCCAAAGCGTTCGCAGCAGGAGTTATCCTCGCAACAGGGTTCGTTCACATGCTCTCGGGCGGATCCAAAGCTTTGTCCGACCCATGTCTGCCGGAGTTTCCATGGAAAATGTTCCCTTTTCCAGAATTCTTCGCCATGGTCGCGGCGCTTCTTACTCTGCTCGCGGATTTCATGATCACGGGATACTACGAGAGGAAACAGGAGAAGATGATGAACCAGTCCGTCGAGTCGCTTGGTACGCAAGTTTCGGTTATGTCTGACCCAGGTCTTGAATCGGGTTTTCTGAGAGATCAAGAAGATGGAGGGGCGTTGCATATCGTGGGGATGCGGGCTCACGCGGAGCATCACCGTCATAGCCTCTCCATGGGTGCCGAAGGTTTTGAGGCTCTCTCTAAGAGAAGTGGTGTTTCCGGTCATGGACACGGCCACAGCCACGGCCACGGGGACGTAGGCTTGGACAGTGGTGTGAGGCACGTTGTTGTTTCTCAGATACTTGAAATGGGAATAGTGTCACATTCAATAATCATAGGAATATCGCTTGGAGTATCACATAGCCCATGCACCATACGTCCTCTCCTTCTCGCTCTTTCCTTCCACCAATTCTTCGAAGGTTTTGCCCTCGGTGGCTGCGTCGCAGAGGCCAGACTAACCCCGCGTGGATCTGCCATGATGGCTTTCTTCTTCGCCATCACTACTCCGATCGGTGTAGCGGTGGGAACGGCCATTGCCTCATCTTACAACTCTTATAGCGTTGCAGCATTGGTGGCTGAAGGAGTGCTCGATTCTCTCTCTGCGGGGATACTTGTGTACATGGCGCTTGTGGATTTGATCGCTGCGGATTTCTTGAGCAAGAAGATGAGTGTTGATTTCAGAGTTCAAGTTGTTTCTTATTGCTTTTTGTTTCTTGGAGCTGGGATGATGTCTGCTCTTGCAATTTGGGCTTGA

Protein Analysis

344

Amino Acids

36.15

Weight (kDa)

6.18

Isoelectric Point (pI)

29.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 1 - 341 8.2e-65 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 458
AccII CGCG 4 cut(s) 233, 254, 428, 745
AccIII TCCGGA 1 cut(s) 20
AciI CCGC 9 cut(s) 150, 233, 254, 418, 428, 743, 800, 884, 926
AclWI GGATC 4 cut(s) 4, 147, 160, 757
AcoI YGGCCR 3 cut(s) 514, 526, 810
AcsI RAATTY 1 cut(s) 215
AcuI CTGAAG 1 cut(s) 879
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 328, 899
AfiI CCNNNNNNNGG 3 cut(s) 50, 71, 454
AgsI TTSAA 4 cut(s) 362, 587, 609, 968
AjnI CCWGG 1 cut(s) 352
AloI GAACNNNNNNTCC 2 cut(s) 46, 78
AluBI AGCT 3 cut(s) 26, 161, 1001
AluI AGCT 3 cut(s) 26, 161, 1001
Alw21I GWGCWC 1 cut(s) 870
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 4 cut(s) 4, 147, 160, 757
Ama87I CYCGRG 1 cut(s) 145
Aor13HI TCCGGA 1 cut(s) 20
AoxI GGCC 4 cut(s) 514, 526, 729, 810
ApeKI GCWGC 5 cut(s) 26, 104, 717, 845, 923
ApoI RAATTY 1 cut(s) 215
Asp700I GAANNNNTTC 1 cut(s) 200
AspLEI GCGC 2 cut(s) 238, 907
AsuHPI GGTGA 1 cut(s) 428
AsuII TTCGAA 1 cut(s) 696
AvaI CYCGRG 1 cut(s) 145
BaeI ACNNNNGTAYC 4 cut(s) 318, 351, 881, 914
BamHI GGATCC 1 cut(s) 152
BanI GGYRCC 1 cut(s) 458
BanII GRGCYC 1 cut(s) 424
Bbv12I GWGCWC 1 cut(s) 870
BbvI GCAGC 5 cut(s) 13, 116, 704, 857, 910
BccI CCATC 3 cut(s) 383, 754, 785
BceAI ACGGC 3 cut(s) 529, 541, 825
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 1 cut(s) 354
BciVI GTATCC 2 cut(s) 266, 882
BclI TGATCA 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 47
BfoI RGCGCY 2 cut(s) 239, 908
BfuI GTATCC 2 cut(s) 266, 882
BglI GCCNNNNNGGC 1 cut(s) 761
BisI GCNGC 6 cut(s) 27, 105, 234, 718, 846, 924
BlsI GCNGC 6 cut(s) 28, 106, 235, 719, 847, 925
Bme1390I CCNGG 1 cut(s) 354
BmeT110I CYCGRG 1 cut(s) 145
BmiI GGNNCC 4 cut(s) 55, 91, 154, 460
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 2 cut(s) 405, 442
BoxI GACNNNNGTC 2 cut(s) 174, 354
BplI GAGNNNNNCTC 4 cut(s) 102, 134, 464, 496
Bpu14I TTCGAA 1 cut(s) 696
BpuEI CTTGAG 1 cut(s) 955
BsaBI GATNNNNATC 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 7 cut(s) 191, 225, 352, 453, 523, 529, 709
BsaWI WCCGGW 2 cut(s) 20, 500
BsaXI ACNNNNNCTCC 2 cut(s) 648, 678
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 71, 454
Bse1I ACTGG 1 cut(s) 307
Bse3DI GCAATG 1 cut(s) 813
Bse8I GATNNNNATC 1 cut(s) 14
BseAI TCCGGA 1 cut(s) 20
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 7 cut(s) 191, 225, 352, 453, 523, 529, 709
BseGI GGATG 2 cut(s) 420, 1011
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 3 cut(s) 50, 71, 454
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 2 cut(s) 365, 590
BseNI ACTGG 1 cut(s) 307
BseXI GCAGC 5 cut(s) 13, 116, 704, 857, 910
BseYI CCCAGC 1 cut(s) 1001
Bsh1236I CGCG 4 cut(s) 233, 254, 428, 745
Bsh1285I CGRYCG 1 cut(s) 792
BshFI GGCC 4 cut(s) 516, 528, 731, 812
BshNI GGYRCC 1 cut(s) 458
BsiEI CGRYCG 1 cut(s) 792
BsiHKAI GWGCWC 1 cut(s) 870
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 4 cut(s) 21, 30, 182, 501
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 3 cut(s) 50, 71, 454
BsmAI GTCTC 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 548
BsnI GGCC 4 cut(s) 516, 528, 731, 812
BsoBI CYCGRG 1 cut(s) 145
Bsp119I TTCGAA 1 cut(s) 696
Bsp1286I GDGCHC 2 cut(s) 424, 870
Bsp13I TCCGGA 1 cut(s) 20
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 7 cut(s) 9, 152, 264, 379, 749, 789, 918
Bsp19I CCATGG 3 cut(s) 191, 225, 453
BspACI CCGC 9 cut(s) 150, 233, 254, 418, 428, 743, 800, 884, 926
BspANI GGCC 4 cut(s) 516, 528, 731, 812
BspCNI CTCAG 2 cut(s) 366, 589
BspEI TCCGGA 1 cut(s) 20
BspFNI CGCG 4 cut(s) 233, 254, 428, 745
BspHI TCATGA 1 cut(s) 261
BspLI GGNNCC 4 cut(s) 55, 91, 154, 460
BspPI GGATC 4 cut(s) 4, 147, 160, 757
BspT104I TTCGAA 1 cut(s) 696
BspT107I GGYRCC 1 cut(s) 458
BsrDI GCAATG 1 cut(s) 813
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 1 cut(s) 307
BssECI CCNNGG 7 cut(s) 191, 225, 352, 453, 523, 529, 709
BssMI GATC 7 cut(s) 9, 152, 264, 379, 749, 789, 918
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 191, 225, 453
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 3 cut(s) 74, 440, 551
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 897
BstBI TTCGAA 1 cut(s) 696
BstC8I GCNNGC 2 cut(s) 252, 420
BstDEI CTNAG 3 cut(s) 374, 483, 576
BstDSI CCRYGG 5 cut(s) 191, 225, 453, 523, 529
BstF5I GGATG 2 cut(s) 420, 1011
BstFNI CGCG 4 cut(s) 233, 254, 428, 745
BstH2I RGCGCY 2 cut(s) 239, 908
BstHHI GCGC 2 cut(s) 238, 907
BstKTI GATC 7 cut(s) 12, 155, 267, 382, 752, 792, 921
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 7 cut(s) 9, 152, 264, 379, 749, 789, 918
BstMCI CGRYCG 1 cut(s) 792
BstMWI GCNNNNNNNGC 6 cut(s) 104, 230, 328, 761, 854, 1028
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 142
BstPAI GACNNNNGTC 2 cut(s) 174, 354
BstSCI CCNGG 1 cut(s) 352
BstUI CGCG 4 cut(s) 233, 254, 428, 745
BstV1I GCAGC 5 cut(s) 13, 116, 704, 857, 910
BstX2I RGATCY 2 cut(s) 152, 749
BstYI RGATCY 2 cut(s) 152, 749
BsuI GTATCC 2 cut(s) 266, 882
BsuRI GGCC 4 cut(s) 516, 528, 731, 812
BtgI CCRYGG 5 cut(s) 191, 225, 453, 523, 529
BtsCI GGATG 2 cut(s) 420, 1011
BtsIMutI CAGTG 1 cut(s) 556
Cac8I GCNNGC 2 cut(s) 252, 420
CciI TCATGA 1 cut(s) 261
CfoI GCGC 2 cut(s) 238, 907
CseI GACGC 1 cut(s) 709
Csp6I GTAC 2 cut(s) 327, 898
CviQI GTAC 2 cut(s) 327, 898
DdeI CTNAG 3 cut(s) 374, 483, 576
DpnI GATC 7 cut(s) 11, 154, 266, 381, 751, 791, 920
DpnII GATC 7 cut(s) 9, 152, 264, 379, 749, 789, 918
EaeI YGGCCR 3 cut(s) 514, 526, 810
EciI GGCGGA 1 cut(s) 165
Eco130I CCWWGG 3 cut(s) 191, 225, 453
Eco24I GRGCYC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 879
Eco88I CYCGRG 1 cut(s) 145
EcoRI GAATTC 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 352
EcoT14I CCWWGG 3 cut(s) 191, 225, 453
EcoT38I GRGCYC 1 cut(s) 424
ErhI CCWWGG 3 cut(s) 191, 225, 453
FaqI GGGAC 1 cut(s) 548
FauI CCCGC 3 cut(s) 411, 750, 877
FbaI TGATCA 1 cut(s) 264
Fnu4HI GCNGC 6 cut(s) 27, 105, 234, 718, 846, 924
FokI GGATG 2 cut(s) 427, 1018
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 6 cut(s) 27, 105, 234, 718, 846, 924
FspBI CTAG 1 cut(s) 47
GlaI GCGC 2 cut(s) 237, 906
GluI GCNGC 6 cut(s) 27, 105, 234, 718, 846, 924
GsaI CCCAGC 1 cut(s) 1005
HaeII RGCGCY 2 cut(s) 239, 908
HaeIII GGCC 4 cut(s) 516, 528, 731, 812
HapII CCGG 4 cut(s) 21, 30, 182, 501
HgaI GACGC 1 cut(s) 709
HhaI GCGC 2 cut(s) 238, 907
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 236, 905
HinP1I GCGC 2 cut(s) 236, 905
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 5 cut(s) 33, 40, 317, 362, 872
HpaII CCGG 4 cut(s) 21, 30, 182, 501
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 2 cut(s) 136, 898
Hpy188I TCNGA 6 cut(s) 169, 349, 375, 579, 789, 962
Hpy188III TCNNGA 7 cut(s) 21, 37, 212, 262, 359, 383, 934
Hpy8I GTNNAC 2 cut(s) 136, 898
Hpy99I CGWCG 3 cut(s) 10, 317, 725
HpyAV CCTTC 5 cut(s) 458, 677, 691, 692, 854
HpyCH4III ACNGT 3 cut(s) 74, 440, 551
HpyCH4IV ACGT 3 cut(s) 537, 564, 658
HpyCH4V TGCA 4 cut(s) 403, 651, 845, 1022
HpyF10VI GCNNNNNNNGC 6 cut(s) 104, 230, 328, 761, 854, 1028
HpyF3I CTNAG 3 cut(s) 374, 483, 576
HpySE526I ACGT 3 cut(s) 537, 564, 658
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 236, 905
Kpn2I TCCGGA 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 264
Kzo9I GATC 7 cut(s) 9, 152, 264, 379, 749, 789, 918
LmnI GCTCC 4 cut(s) 23, 89, 430, 998
Lsp1109I GCAGC 5 cut(s) 13, 116, 704, 857, 910
LweI GCATC 2 cut(s) 405, 442
MaeI CTAG 1 cut(s) 47
MaeII ACGT 3 cut(s) 537, 564, 658
MaeIII GTNAC 1 cut(s) 600
MalI GATC 7 cut(s) 11, 154, 266, 381, 751, 791, 920
MboI GATC 7 cut(s) 9, 152, 264, 379, 749, 789, 918
MboII GAAGA 7 cut(s) 211, 307, 398, 685, 760, 763, 955
MflI RGATCY 2 cut(s) 152, 749
MhlI GDGCHC 2 cut(s) 424, 870
MluCI AATT 3 cut(s) 215, 689, 1023
MlyI GAGTC 2 cut(s) 42, 326
MmeI TCCRAC 2 cut(s) 31, 192
MroI TCCGGA 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 200
MseI TTAA 2 cut(s) 68, 80
MspI CCGG 4 cut(s) 21, 30, 182, 501
MspR9I CCNGG 1 cut(s) 354
MvaI CCWGG 1 cut(s) 354
MvnI CGCG 4 cut(s) 233, 254, 428, 745
MwoI GCNNNNNNNGC 6 cut(s) 104, 230, 328, 761, 854, 1028
NcoI CCATGG 3 cut(s) 191, 225, 453
NdeII GATC 7 cut(s) 9, 152, 264, 379, 749, 789, 918
NlaIV GGNNCC 4 cut(s) 55, 91, 154, 460
NmuCI GTSAC 1 cut(s) 600
NspI RCATGY 1 cut(s) 142
NspV TTCGAA 1 cut(s) 696
PagI TCATGA 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 717
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 3 cut(s) 40, 362, 872
PkrI GCNGC 6 cut(s) 28, 106, 235, 719, 847, 925
Ple19I CGATCG 1 cut(s) 792
PleI GAGTC 2 cut(s) 41, 325
PpsI GAGTC 2 cut(s) 41, 325
PshAI GACNNNNGTC 2 cut(s) 174, 354
Psp6I CCWGG 1 cut(s) 352
PspFI CCCAGC 1 cut(s) 1001
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 4 cut(s) 55, 91, 154, 460
PsuI RGATCY 2 cut(s) 152, 749
PvuI CGATCG 1 cut(s) 792
RsaI GTAC 2 cut(s) 328, 899
RsaNI GTAC 2 cut(s) 327, 898
SaqAI TTAA 2 cut(s) 68, 80
SatI GCNGC 6 cut(s) 27, 105, 234, 718, 846, 924
Sau3AI GATC 7 cut(s) 9, 152, 264, 379, 749, 789, 918
SchI GAGTC 2 cut(s) 42, 326
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 2 cut(s) 424, 870
SetI ASST 9 cut(s) 28, 163, 358, 469, 540, 567, 661, 703, 1003
SfaNI GCATC 2 cut(s) 405, 442
SfuI TTCGAA 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 934
SmoI CTYRAG 1 cut(s) 934
Sse9I AATT 3 cut(s) 215, 689, 1023
SsiI CCGC 9 cut(s) 150, 233, 254, 418, 428, 743, 800, 884, 926
SspMI CTAG 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 3 cut(s) 191, 225, 453
TaaI ACNGT 3 cut(s) 74, 440, 551
TaiI ACGT 3 cut(s) 540, 567, 661
TaqI TCGA 5 cut(s) 8, 38, 315, 696, 870
TasI AATT 3 cut(s) 215, 689, 1023
TatI WGTACW 1 cut(s) 897
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 3 cut(s) 40, 362, 872
Tru1I TTAA 2 cut(s) 68, 80
Tru9I TTAA 2 cut(s) 68, 80
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 1 cut(s) 600
TseI GCWGC 5 cut(s) 26, 104, 717, 845, 923
Tsp45I GTSAC 1 cut(s) 600
TspDTI ATGAA 2 cut(s) 250, 317
TspGWI ACGGA 2 cut(s) 54, 301
TspRI CASTG 1 cut(s) 556
XapI RAATTY 1 cut(s) 215
XceI RCATGY 1 cut(s) 142
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.