AT4G35590

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009509
Physical Location & Seq
Forward (+)
16892909 .. 16895105
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35590.1

Sequence Viewer

Length: 1113 bp
ATGGTGGACCAAGGATTTTTCACACTAAAAAAGGAAAAAAAGAAAAATATATTAATAAAACTTTTTTATGTTAAAATCTTGGGCTTCTGCTTTTGCGACTCTTGGTCTTCTTCGGACATGGCACATTCCTTAACCTCACTCGCCGTTTTCCAGAGCGTCATCCGCAAAGAGATGGTGAGGAGTTTGCATGTCTATGAATCGGTGGAGATTGAGAGAGAGTTCTGGTTCAAGAGCAAAAGCTGTTATGTAGAGAAGAAAGCGAAGCCTCTGTTTCGTTCGGAAGATTTCCGGCGACCGGAGATCTCGGAAGGGTCGGTTTTTGGCACGTGGCGTTGTATCTTTGTGTTCCGGTTTAATCACTCGCTTCCTCGGTTTCCTACTCTTCTCTGTCTTTCCAGAAATCCCAAACTGGAGGACATCCCTAATTTAGCCAACGAGCTCAAGTTTATCTCCGAGTTAAAACCATCAAAGATTTATGAAGAAGAACAATGCAGTAGCAGTACAGAGGGATATTATAACTCTGATCTGCCTAAACCACGAAAGCTCGTTCTGAAACAAGATCTTAACTGCCTTCCTGATTCAGAAACCGAATCCGAGGAATCTGTAAACGAAAAAACCGAACATTCGGAATTTGAAAACGATAAAACTGAACAGTCGGAATCAGATGCTAAGACTGAGATTTTGAAGAAGAAGAAGAGGACACCATCGAGACATGTTGCTGAACTATCCTTAGAAGAGCTTTCAAAATACTTTGACCTCACTATCGTGGAAGCTTCTCGGAATCTCAAGGTCGGTCTCACTGTTTTGAAAAAGAAATGCAGAGAGTTTGGGATTCCACGGTGGCCTCATAGGAAGATCAAATCTCTCGACTGTCTCATCCACGATCTTCAGAGGGAAGCAGAGAAGCAGCAGGAAAAGAATGAAGCAGCAGCAATGGCGGTAGCTAAGAAACAGGAGAAACTGGAGACAGAGAAGAGAAATATAGTGAAGAGACCATTCATGGAGATAGGGATAGAAACCAAAAAATTCAGACAAGAAAACTTCAAGAAAAGACACAGGGCTTCTAGAGCCAAGAAGAATCAAGAATCTCTTGTCACTTCCTCTTCCACTTAA

Protein Analysis

370

Amino Acids

43.36

Weight (kDa)

9.2

Isoelectric Point (pI)

58.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 242 - 289 9.4e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 516
AciI CCGC 2 cut(s) 163, 938
AcsI RAATTY 2 cut(s) 629, 1025
AcuI CTGAAG 1 cut(s) 872
AcvI CACGTG 1 cut(s) 327
AfaI GTAC 1 cut(s) 502
AfiI CCNNNNNNNGG 1 cut(s) 295
AflIII ACRYGT 1 cut(s) 712
AgsI TTSAA 6 cut(s) 229, 635, 685, 744, 808, 1045
AhdI GACNNNNNGTC 1 cut(s) 103
AleI CACNNNNGTG 1 cut(s) 764
AluBI AGCT 6 cut(s) 240, 439, 544, 739, 773, 944
AluI AGCT 6 cut(s) 240, 439, 544, 739, 773, 944
Alw21I GWGCWC 1 cut(s) 441
Alw26I GTCTC 5 cut(s) 703, 800, 878, 959, 985
AoxI GGCC 1 cut(s) 842
ApeKI GCWGC 3 cut(s) 907, 926, 929
ApoI RAATTY 2 cut(s) 629, 1025
ArsI GACNNNNNNTTYG 2 cut(s) 664, 696
AseI ATTAAT 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 1 cut(s) 187
AvaII GGWCC 1 cut(s) 7
BanII GRGCYC 1 cut(s) 441
BbrPI CACGTG 1 cut(s) 327
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 3 cut(s) 919, 938, 941
BccI CCATC 3 cut(s) 166, 472, 712
BceAI ACGGC 1 cut(s) 128
BcoDI GTCTC 5 cut(s) 703, 800, 878, 959, 985
BfaI CTAG 1 cut(s) 1065
BglII AGATCT 2 cut(s) 300, 559
BisI GCNGC 3 cut(s) 908, 927, 930
BlsI GCNGC 3 cut(s) 909, 928, 931
Bme18I GGWCC 1 cut(s) 7
BmeRI GACNNNNNGTC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 7
BmsI GCATC 1 cut(s) 655
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 2 cut(s) 431, 983
BpuEI CTTGAG 2 cut(s) 425, 770
BsaAI YACGTR 1 cut(s) 327
BsaI GGTCTC 2 cut(s) 800, 985
BsaJI CCNNGG 4 cut(s) 10, 368, 594, 836
BsaWI WCCGGW 2 cut(s) 295, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse1I ACTGG 2 cut(s) 414, 966
Bse3DI GCAATG 1 cut(s) 939
BseDI CCNNGG 4 cut(s) 10, 368, 594, 836
BseGI GGATG 3 cut(s) 159, 417, 876
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMI GCAATG 1 cut(s) 939
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 2 cut(s) 414, 966
BseRI GAGGAG 1 cut(s) 193
BseXI GCAGC 3 cut(s) 919, 938, 941
Bsh1285I CGRYCG 1 cut(s) 296
BshFI GGCC 1 cut(s) 844
BsiEI CGRYCG 1 cut(s) 296
BsiHKAI GWGCWC 1 cut(s) 441
BsiSI CCGG 3 cut(s) 289, 296, 349
BslI CCNNNNNNNGG 1 cut(s) 295
BsmAI GTCTC 5 cut(s) 703, 800, 878, 959, 985
BsnI GGCC 1 cut(s) 844
Bso31I GGTCTC 2 cut(s) 800, 985
Bsp1286I GDGCHC 1 cut(s) 441
Bsp143I GATC 5 cut(s) 300, 523, 559, 855, 883
BspACI CCGC 2 cut(s) 163, 938
BspANI GGCC 1 cut(s) 844
BspCNI CTCAG 1 cut(s) 667
BspQI GCTCTTC 1 cut(s) 729
BspTNI GGTCTC 2 cut(s) 800, 985
BsrDI GCAATG 1 cut(s) 939
BsrI ACTGG 2 cut(s) 414, 966
BssECI CCNNGG 4 cut(s) 10, 368, 594, 836
BssMI GATC 5 cut(s) 300, 523, 559, 855, 883
BssT1I CCWWGG 1 cut(s) 10
Bst4CI ACNGT 4 cut(s) 654, 802, 840, 872
Bst6I CTCTTC 6 cut(s) 387, 689, 729, 968, 983, 1108
BstBAI YACGTR 1 cut(s) 327
BstDEI CTNAG 4 cut(s) 669, 675, 730, 945
BstDSI CCRYGG 1 cut(s) 836
BstF5I GGATG 3 cut(s) 159, 417, 876
BstKTI GATC 5 cut(s) 303, 526, 562, 858, 886
BstMAI GTCTC 5 cut(s) 703, 800, 878, 959, 985
BstMBI GATC 5 cut(s) 300, 523, 559, 855, 883
BstMCI CGRYCG 1 cut(s) 296
BstMWI GCNNNNNNNGC 3 cut(s) 162, 935, 1067
BstNSI RCATGY 2 cut(s) 191, 716
BstV1I GCAGC 3 cut(s) 919, 938, 941
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 2 cut(s) 300, 559
BstYI RGATCY 2 cut(s) 300, 559
BsuRI GGCC 1 cut(s) 844
BtgI CCRYGG 1 cut(s) 836
BtsCI GGATG 3 cut(s) 159, 417, 876
BtsIMutI CAGTG 1 cut(s) 798
Cfr13I GGNCC 1 cut(s) 7
CseI GACGC 1 cut(s) 145
Csp6I GTAC 1 cut(s) 501
CviAII CATG 4 cut(s) 118, 188, 713, 1000
CviQI GTAC 1 cut(s) 501
DdeI CTNAG 4 cut(s) 669, 675, 730, 945
DpnI GATC 5 cut(s) 302, 525, 561, 857, 885
DpnII GATC 5 cut(s) 300, 523, 559, 855, 883
DriI GACNNNNNGTC 1 cut(s) 103
Eam1104I CTCTTC 6 cut(s) 387, 689, 729, 968, 983, 1108
Eam1105I GACNNNNNGTC 1 cut(s) 103
EarI CTCTTC 6 cut(s) 387, 689, 729, 968, 983, 1108
Ecl136II GAGCTC 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 441
Eco31I GGTCTC 2 cut(s) 800, 985
Eco47I GGWCC 1 cut(s) 7
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 872
Eco72I CACGTG 1 cut(s) 327
EcoICRI GAGCTC 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 10
EcoT38I GRGCYC 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 4 cut(s) 121, 191, 716, 1003
FalI AAGNNNNNCTT 2 cut(s) 1074, 1106
FatI CATG 4 cut(s) 117, 187, 712, 999
Fnu4HI GCNGC 3 cut(s) 908, 927, 930
FokI GGATG 3 cut(s) 146, 404, 863
FriOI GRGCYC 1 cut(s) 441
Fsp4HI GCNGC 3 cut(s) 908, 927, 930
FspBI CTAG 1 cut(s) 1065
GluI GCNGC 3 cut(s) 908, 927, 930
GsuI CTGGAG 2 cut(s) 431, 983
HaeIII GGCC 1 cut(s) 844
HapII CCGG 3 cut(s) 289, 296, 349
HgaI GACGC 1 cut(s) 145
Hin1II CATG 4 cut(s) 121, 191, 716, 1003
HindIII AAGCTT 1 cut(s) 771
HpaII CCGG 3 cut(s) 289, 296, 349
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 2 cut(s) 7, 607
Hpy188III TCNNGA 9 cut(s) 151, 229, 396, 575, 708, 866, 1045, 1065, 1082
Hpy8I GTNNAC 2 cut(s) 7, 607
HpyAV CCTTC 2 cut(s) 302, 581
HpyCH4III ACNGT 4 cut(s) 654, 802, 840, 872
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 3 cut(s) 187, 492, 819
HpyF10VI GCNNNNNNNGC 3 cut(s) 162, 935, 1067
HpyF3I CTNAG 4 cut(s) 669, 675, 730, 945
HpySE526I ACGT 1 cut(s) 326
Hsp92II CATG 4 cut(s) 121, 191, 716, 1003
Kzo9I GATC 5 cut(s) 300, 523, 559, 855, 883
LguI GCTCTTC 1 cut(s) 729
Lsp1109I GCAGC 3 cut(s) 919, 938, 941
LweI GCATC 1 cut(s) 655
MaeI CTAG 1 cut(s) 1065
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 1 cut(s) 1093
MalI GATC 5 cut(s) 302, 525, 561, 857, 885
MboI GATC 5 cut(s) 300, 523, 559, 855, 883
MflI RGATCY 2 cut(s) 300, 559
MhlI GDGCHC 1 cut(s) 441
MluCI AATT 3 cut(s) 424, 629, 1025
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 636
MseI TTAA 7 cut(s) 53, 72, 131, 354, 458, 564, 1111
MslI CAYNNNNRTG 2 cut(s) 192, 764
MspI CCGG 3 cut(s) 289, 296, 349
MwoI GCNNNNNNNGC 3 cut(s) 162, 935, 1067
NdeII GATC 5 cut(s) 300, 523, 559, 855, 883
NlaIII CATG 4 cut(s) 121, 191, 716, 1003
NmuCI GTSAC 1 cut(s) 1093
NspI RCATGY 2 cut(s) 191, 716
OliI CACNNNNGTG 1 cut(s) 764
PciI ACATGT 1 cut(s) 712
PciSI GCTCTTC 1 cut(s) 729
PcsI WCGNNNNNNNCGW 1 cut(s) 615
PfeI GAWTC 9 cut(s) 197, 578, 590, 599, 659, 781, 832, 1078, 1085
PkrI GCNGC 3 cut(s) 909, 928, 931
PleI GAGTC 1 cut(s) 92
PmaCI CACGTG 1 cut(s) 327
PmlI CACGTG 1 cut(s) 327
PpsI GAGTC 1 cut(s) 92
Ppu21I YACGTR 1 cut(s) 327
PscI ACATGT 1 cut(s) 712
PshBI ATTAAT 1 cut(s) 53
PsiI TTATAA 1 cut(s) 516
Psp124BI GAGCTC 1 cut(s) 441
PspCI CACGTG 1 cut(s) 327
PspPI GGNCC 1 cut(s) 7
PsuI RGATCY 2 cut(s) 300, 559
RsaI GTAC 1 cut(s) 502
RsaNI GTAC 1 cut(s) 501
RseI CAYNNNNRTG 2 cut(s) 192, 764
SacI GAGCTC 1 cut(s) 441
SapI GCTCTTC 1 cut(s) 729
SaqAI TTAA 7 cut(s) 53, 72, 131, 354, 458, 564, 1111
SatI GCNGC 3 cut(s) 908, 927, 930
Sau3AI GATC 5 cut(s) 300, 523, 559, 855, 883
Sau96I GGNCC 1 cut(s) 7
SchI GAGTC 1 cut(s) 92
SduI GDGCHC 1 cut(s) 441
SfaNI GCATC 1 cut(s) 655
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 2 cut(s) 192, 764
SmlI CTYRAG 2 cut(s) 440, 785
SmoI CTYRAG 2 cut(s) 440, 785
Sse9I AATT 3 cut(s) 424, 629, 1025
SsiI CCGC 2 cut(s) 163, 938
SspMI CTAG 1 cut(s) 1065
SstI GAGCTC 1 cut(s) 441
StyI CCWWGG 1 cut(s) 10
TaaI ACNGT 4 cut(s) 654, 802, 840, 872
TaiI ACGT 1 cut(s) 329
TaqI TCGA 2 cut(s) 707, 867
TaqII GACCGA 1 cut(s) 782
TasI AATT 3 cut(s) 424, 629, 1025
TatI WGTACW 1 cut(s) 500
TfiI GAWTC 9 cut(s) 197, 578, 590, 599, 659, 781, 832, 1078, 1085
Tru1I TTAA 7 cut(s) 53, 72, 131, 354, 458, 564, 1111
Tru9I TTAA 7 cut(s) 53, 72, 131, 354, 458, 564, 1111
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 1 cut(s) 1093
TseI GCWGC 3 cut(s) 907, 926, 929
Tsp45I GTSAC 1 cut(s) 1093
TspDTI ATGAA 4 cut(s) 210, 492, 936, 988
TspRI CASTG 1 cut(s) 805
VpaK11BI GGWCC 1 cut(s) 7
VspI ATTAAT 1 cut(s) 53
XapI RAATTY 2 cut(s) 629, 1025
XbaI TCTAGA 1 cut(s) 1064
XceI RCATGY 2 cut(s) 191, 716
XspI CTAG 1 cut(s) 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.