MD15G1232500.v1.1

Rab proteins geranylgeranyltransferase component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009509
Physical Location & Seq
Forward (+)
19001116 .. 19004487
3372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1232500.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGATTCCTCTGACTGTGACCCCCATTTGCTGAGATCTCTTCTGGTCTTCAAGAACACCATCAACCAGGAGTTGATCAGGAGCTTGCATGTGTACGGATTGGATGAGGGGAAGAGAAATGAGGTGGAGAGGGAGTTTGTGTTCAGAGAAAATGGGTTGTATGTGGAGCTGTCAGCTGAGCCGGTTCTGAGACTGATGAAATTTCCAGCTTCCCAAGTGCTCCAAGGGCATGTCAATGGCTGCTGGGTCTGCATTTTAGCATTTCATGAAAATTCACCTCCTGATTTTACATGCATTCCCTCTGTTCTTTCAGTCTCCAGAAATCCAAAGTTGAAGACAATTCCAAGGTTGTCTAATGATCTTCAGATGATTTTCAAGTTGAGTTGCAAAACAGATGACAAAAAATCATTGCAAAGGTCACGGGAGGAAACAAGCCAAGGGAGTAATCATAGTCACCACAAATCAAGGAGGTGGCCGATACTTGATCTGGATCTGAACTCTCTTCCTAGCCCCGTCACAATGTCTGAATCATCTGAGGATCAAGAAAGTGGAAGAAGTTCACCAGGTATTGCAGAGAAGAAAAGAAGGGCACCAAGTGGACGCATAGCAAGTATCGCCTTGTCAGATCTGGCAAAGTATTTTGATCTACCAATTGTGGAAGCTTCGAGAAATCTGAATGTTGGACTCACAGTACTTAAGAAGAAATGCAGAGAATTTGGTATTCCTCGGTGGCCACATAGGAAGATTAAATCGCTTGACAGTCTCATTCGTGATCTTCAGGAAGAGACGGAGGTCCAACAGAAGGAGAACAAGGCCGCAGCCTTGGCAGTTCTAAAGAGGCAAAGAATGCTGGAGAGGGAAAGAGAAAGTATAGAGAGGAGACCCTTCTTGGAGATGAAGACCGAGACCAAAAGATTCCGGCAAGATGTTTTCAAGAGACGGCACAGAGCTAGAGCTCTTAGAAGCCAGGGCCTGTCTACCTCTGCCAATTAA

Protein Analysis

331

Amino Acids

37.95

Weight (kDa)

9.69

Isoelectric Point (pI)

68.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 204 - 252 4.1e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 589
AccI GTMKAC 1 cut(s) 977
AciI CCGC 1 cut(s) 816
AclWI GGATC 2 cut(s) 498, 546
AcoI YGGCCR 2 cut(s) 473, 731
AcsI RAATTY 3 cut(s) 200, 271, 713
AcuI CTGAAG 2 cut(s) 347, 761
AdeI CACNNNGTG 1 cut(s) 596
AfaI GTAC 2 cut(s) 95, 693
AfiI CCNNNNNNNGG 1 cut(s) 802
AflII CTTAAG 1 cut(s) 695
AgsI TTSAA 4 cut(s) 52, 334, 376, 934
AjnI CCWGG 3 cut(s) 66, 562, 966
AloI GAACNNNNNNTCC 2 cut(s) 125, 157
AluBI AGCT 7 cut(s) 84, 169, 176, 209, 662, 950, 956
AluI AGCT 7 cut(s) 84, 169, 176, 209, 662, 950, 956
Alw21I GWGCWC 2 cut(s) 222, 958
Alw26I GTCTC 7 cut(s) 184, 319, 767, 779, 874, 899, 931
AlwI GGATC 2 cut(s) 498, 546
AlwNI CAGNNNCTG 1 cut(s) 973
AoxI GGCC 4 cut(s) 473, 731, 813, 970
ApeKI GCWGC 2 cut(s) 240, 818
ApoI RAATTY 3 cut(s) 200, 271, 713
Asp700I GAANNNNTTC 1 cut(s) 556
AspS9I GGNCC 2 cut(s) 793, 970
AsuHPI GGTGA 3 cut(s) 267, 446, 552
AvaII GGWCC 1 cut(s) 793
BaeGI GKGCMC 1 cut(s) 592
BalI TGGCCA 1 cut(s) 733
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 1 cut(s) 958
BbsI GAAGAC 3 cut(s) 40, 341, 905
Bbv12I GWGCWC 2 cut(s) 222, 958
BbvI GCAGC 2 cut(s) 227, 830
BccI CCATC 1 cut(s) 68
BceAI ACGGC 1 cut(s) 956
BciT130I CCWGG 3 cut(s) 68, 564, 968
BclI TGATCA 1 cut(s) 75
BcoDI GTCTC 7 cut(s) 184, 319, 767, 779, 874, 899, 931
BfaI CTAG 2 cut(s) 507, 951
BfrI CTTAAG 1 cut(s) 695
BglII AGATCT 2 cut(s) 35, 625
BisI GCNGC 3 cut(s) 241, 816, 819
BlpI GCTNAGC 1 cut(s) 177
BlsI GCNGC 3 cut(s) 242, 817, 820
BmcAI AGTACT 1 cut(s) 693
Bme1390I CCNGG 3 cut(s) 68, 564, 968
Bme18I GGWCC 1 cut(s) 793
BmgT120I GGNCC 2 cut(s) 793, 970
BmiI GGNNCC 1 cut(s) 591
BmrFI CCNGG 3 cut(s) 68, 564, 968
BoxI GACNNNNGTC 1 cut(s) 791
BpiI GAAGAC 3 cut(s) 40, 341, 905
BpmI CTGGAG 2 cut(s) 301, 872
Bpu1102I GCTNAGC 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 489
BsaI GGTCTC 2 cut(s) 874, 899
BsaJI CCNNGG 6 cut(s) 223, 344, 436, 725, 822, 967
BsaXI ACNNNNNCTCC 4 cut(s) 119, 125, 149, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 802
Bse118I RCCGGY 1 cut(s) 181
Bse3DI GCAATG 1 cut(s) 407
Bse8I GATNNNNATC 1 cut(s) 489
BseBI CCWGG 3 cut(s) 68, 564, 968
BseDI CCNNGG 6 cut(s) 223, 344, 436, 725, 822, 967
BseGI GGATG 1 cut(s) 109
BseJI GATNNNNATC 1 cut(s) 489
BseLI CCNNNNNNNGG 1 cut(s) 802
BseMI GCAATG 1 cut(s) 407
BseMII CTCAG 4 cut(s) 23, 168, 179, 525
BseRI GAGGAG 1 cut(s) 892
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 2 cut(s) 227, 830
BseYI CCCAGC 1 cut(s) 243
BshFI GGCC 4 cut(s) 475, 733, 815, 972
BshNI GGYRCC 1 cut(s) 589
BsiHKAI GWGCWC 2 cut(s) 222, 958
BsiSI CCGG 2 cut(s) 182, 919
BslI CCNNNNNNNGG 1 cut(s) 802
BsmAI GTCTC 7 cut(s) 184, 319, 767, 779, 874, 899, 931
BsmBI CGTCTC 2 cut(s) 779, 931
BsmI GAATGC 2 cut(s) 294, 852
BsnI GGCC 4 cut(s) 475, 733, 815, 972
Bso31I GGTCTC 2 cut(s) 874, 899
Bsp1286I GDGCHC 3 cut(s) 222, 592, 958
Bsp143I GATC 9 cut(s) 35, 75, 358, 484, 490, 538, 625, 643, 772
Bsp1720I GCTNAGC 1 cut(s) 177
BspACI CCGC 1 cut(s) 816
BspANI GGCC 4 cut(s) 475, 733, 815, 972
BspCNI CTCAG 4 cut(s) 24, 169, 180, 526
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 591
BspPI GGATC 2 cut(s) 498, 546
BspT107I GGYRCC 1 cut(s) 589
BspTI CTTAAG 1 cut(s) 695
BspTNI GGTCTC 2 cut(s) 874, 899
BsrDI GCAATG 1 cut(s) 407
BsrFI RCCGGY 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 6 cut(s) 223, 344, 436, 725, 822, 967
BssMI GATC 9 cut(s) 35, 75, 358, 484, 490, 538, 625, 643, 772
BssT1I CCWWGG 4 cut(s) 223, 344, 436, 822
Bst2UI CCWGG 3 cut(s) 68, 564, 968
Bst4CI ACNGT 3 cut(s) 17, 691, 761
Bst6I CTCTTC 4 cut(s) 45, 107, 507, 777
BstAFI CTTAAG 1 cut(s) 695
BstAPI GCANNNNNTGC 1 cut(s) 847
BstC8I GCNNGC 1 cut(s) 86
BstDEI CTNAG 5 cut(s) 32, 177, 188, 534, 959
BstF5I GGATG 1 cut(s) 109
BstKTI GATC 9 cut(s) 38, 78, 361, 487, 493, 541, 628, 646, 775
BstMAI GTCTC 7 cut(s) 184, 319, 767, 779, 874, 899, 931
BstMBI GATC 9 cut(s) 35, 75, 358, 484, 490, 538, 625, 643, 772
BstMWI GCNNNNNNNGC 5 cut(s) 226, 249, 614, 824, 847
BstNI CCWGG 3 cut(s) 68, 564, 968
BstNSI RCATGY 3 cut(s) 92, 233, 294
BstPAI GACNNNNGTC 1 cut(s) 791
BstSCI CCNGG 3 cut(s) 66, 562, 966
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 2 cut(s) 227, 830
BstV2I GAAGAC 3 cut(s) 40, 341, 905
BstX2I RGATCY 3 cut(s) 35, 490, 625
BstYI RGATCY 3 cut(s) 35, 490, 625
BsuRI GGCC 4 cut(s) 475, 733, 815, 972
BtsCI GGATG 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 86
CaiI CAGNNNCTG 1 cut(s) 973
CciI TCATGA 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 2 cut(s) 793, 970
CseI GACGC 1 cut(s) 609
CsiI ACCWGGT 1 cut(s) 562
Csp6I GTAC 2 cut(s) 94, 692
CviAII CATG 4 cut(s) 89, 230, 266, 291
CviQI GTAC 2 cut(s) 94, 692
DdeI CTNAG 5 cut(s) 32, 177, 188, 534, 959
DpnI GATC 9 cut(s) 37, 77, 360, 486, 492, 540, 627, 645, 774
DpnII GATC 9 cut(s) 35, 75, 358, 484, 490, 538, 625, 643, 772
DraIII CACNNNGTG 1 cut(s) 596
EaeI YGGCCR 2 cut(s) 473, 731
Eam1104I CTCTTC 4 cut(s) 45, 107, 507, 777
EarI CTCTTC 4 cut(s) 45, 107, 507, 777
Ecl136II GAGCTC 1 cut(s) 956
Eco130I CCWWGG 4 cut(s) 223, 344, 436, 822
Eco24I GRGCYC 1 cut(s) 958
Eco31I GGTCTC 2 cut(s) 874, 899
Eco47I GGWCC 1 cut(s) 793
Eco53kI GAGCTC 1 cut(s) 956
Eco57I CTGAAG 2 cut(s) 347, 761
EcoICRI GAGCTC 1 cut(s) 956
EcoO109I RGGNCCY 1 cut(s) 970
EcoRII CCWGG 3 cut(s) 66, 562, 966
EcoT14I CCWWGG 4 cut(s) 223, 344, 436, 822
EcoT22I ATGCAT 1 cut(s) 296
EcoT38I GRGCYC 1 cut(s) 958
ErhI CCWWGG 4 cut(s) 223, 344, 436, 822
Esp3I CGTCTC 2 cut(s) 779, 931
FaeI CATG 4 cut(s) 92, 233, 269, 294
FaiI YATR 9 cut(s) 90, 162, 231, 267, 292, 450, 605, 738, 872
FatI CATG 4 cut(s) 88, 229, 265, 290
FbaI TGATCA 1 cut(s) 75
FblI GTMKAC 1 cut(s) 977
Fnu4HI GCNGC 3 cut(s) 241, 816, 819
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 958
Fsp4HI GCNGC 3 cut(s) 241, 816, 819
FspBI CTAG 2 cut(s) 507, 951
GluI GCNGC 3 cut(s) 241, 816, 819
GsaI CCCAGC 1 cut(s) 247
GsuI CTGGAG 2 cut(s) 301, 872
HaeIII GGCC 4 cut(s) 475, 733, 815, 972
HapII CCGG 2 cut(s) 182, 919
HgaI GACGC 1 cut(s) 609
Hin1II CATG 4 cut(s) 92, 233, 269, 294
HindIII AAGCTT 1 cut(s) 660
HinfI GANTC 4 cut(s) 5, 527, 684, 915
HpaII CCGG 2 cut(s) 182, 919
HphI GGTGA 3 cut(s) 267, 446, 552
Hpy166II GTNNAC 4 cut(s) 94, 560, 599, 978
Hpy188I TCNGA 9 cut(s) 13, 146, 189, 366, 495, 526, 535, 625, 675
Hpy8I GTNNAC 4 cut(s) 94, 560, 599, 978
HpyAV CCTTC 3 cut(s) 579, 796, 895
HpyCH4III ACNGT 3 cut(s) 17, 691, 761
HpyCH4V TGCA 7 cut(s) 88, 252, 294, 387, 412, 572, 708
HpyF10VI GCNNNNNNNGC 5 cut(s) 226, 249, 614, 824, 847
HpyF3I CTNAG 5 cut(s) 32, 177, 188, 534, 959
Hsp92II CATG 4 cut(s) 92, 233, 269, 294
Ksp22I TGATCA 1 cut(s) 75
Kzo9I GATC 9 cut(s) 35, 75, 358, 484, 490, 538, 625, 643, 772
LmnI GCTCC 3 cut(s) 81, 166, 225
Lsp1109I GCAGC 2 cut(s) 227, 830
MabI ACCWGGT 1 cut(s) 562
MaeI CTAG 2 cut(s) 507, 951
MaeIII GTNAC 4 cut(s) 17, 417, 452, 514
MalI GATC 9 cut(s) 37, 77, 360, 486, 492, 540, 627, 645, 774
MboI GATC 9 cut(s) 35, 75, 358, 484, 490, 538, 625, 643, 772
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 3 cut(s) 35, 490, 625
MhlI GDGCHC 3 cut(s) 222, 592, 958
MlsI TGGCCA 1 cut(s) 733
MluCI AATT 6 cut(s) 200, 271, 339, 651, 713, 988
MluNI TGGCCA 1 cut(s) 733
MlyI GAGTC 1 cut(s) 678
MmeI TCCRAC 2 cut(s) 661, 820
Mox20I TGGCCA 1 cut(s) 733
Mph1103I ATGCAT 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 556
MscI TGGCCA 1 cut(s) 733
MseI TTAA 3 cut(s) 696, 747, 991
MslI CAYNNNNRTG 1 cut(s) 234
Msp20I TGGCCA 1 cut(s) 733
MspA1I CMGCKG 1 cut(s) 176
MspCI CTTAAG 1 cut(s) 695
MspI CCGG 2 cut(s) 182, 919
MspR9I CCNGG 3 cut(s) 68, 564, 968
MunI CAATTG 1 cut(s) 651
Mva1269I GAATGC 2 cut(s) 294, 852
MvaI CCWGG 3 cut(s) 68, 564, 968
MwoI GCNNNNNNNGC 5 cut(s) 226, 249, 614, 824, 847
NdeII GATC 9 cut(s) 35, 75, 358, 484, 490, 538, 625, 643, 772
NlaIII CATG 4 cut(s) 92, 233, 269, 294
NlaIV GGNNCC 1 cut(s) 591
NmuCI GTSAC 4 cut(s) 17, 417, 452, 514
NsiI ATGCAT 1 cut(s) 296
NspI RCATGY 3 cut(s) 92, 233, 294
PagI TCATGA 1 cut(s) 265
PctI GAATGC 2 cut(s) 294, 852
PdmI GAANNNNTTC 1 cut(s) 556
PfeI GAWTC 3 cut(s) 5, 527, 915
PkrI GCNGC 3 cut(s) 242, 817, 820
PleI GAGTC 1 cut(s) 678
PpsI GAGTC 1 cut(s) 678
PshAI GACNNNNGTC 1 cut(s) 791
Psp124BI GAGCTC 1 cut(s) 958
Psp6I CCWGG 3 cut(s) 66, 562, 966
PspFI CCCAGC 1 cut(s) 243
PspGI CCWGG 3 cut(s) 66, 562, 966
PspN4I GGNNCC 1 cut(s) 591
PspPI GGNCC 2 cut(s) 793, 970
PstNI CAGNNNCTG 1 cut(s) 973
PsuI RGATCY 3 cut(s) 35, 490, 625
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 2 cut(s) 95, 693
RsaNI GTAC 2 cut(s) 94, 692
RseI CAYNNNNRTG 1 cut(s) 234
SacI GAGCTC 1 cut(s) 958
SaqAI TTAA 3 cut(s) 696, 747, 991
SatI GCNGC 3 cut(s) 241, 816, 819
Sau3AI GATC 9 cut(s) 35, 75, 358, 484, 490, 538, 625, 643, 772
Sau96I GGNCC 2 cut(s) 793, 970
ScaI AGTACT 1 cut(s) 693
SchI GAGTC 1 cut(s) 678
ScrFI CCNGG 3 cut(s) 68, 564, 968
SduI GDGCHC 3 cut(s) 222, 592, 958
SexAI ACCWGGT 1 cut(s) 562
SinI GGWCC 1 cut(s) 793
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 695
SmoI CTYRAG 1 cut(s) 695
Sse9I AATT 6 cut(s) 200, 271, 339, 651, 713, 988
SsiI CCGC 1 cut(s) 816
SspMI CTAG 2 cut(s) 507, 951
SstI GAGCTC 1 cut(s) 958
StyD4I CCNGG 3 cut(s) 66, 562, 966
StyI CCWWGG 4 cut(s) 223, 344, 436, 822
TaaI ACNGT 3 cut(s) 17, 691, 761
TaqI TCGA 1 cut(s) 665
TaqII GACCGA 1 cut(s) 917
TasI AATT 6 cut(s) 200, 271, 339, 651, 713, 988
TatI WGTACW 1 cut(s) 691
TauI GCSGC 1 cut(s) 818
TfiI GAWTC 3 cut(s) 5, 527, 915
Tru1I TTAA 3 cut(s) 696, 747, 991
Tru9I TTAA 3 cut(s) 696, 747, 991
TseFI GTSAC 4 cut(s) 17, 417, 452, 514
TseI GCWGC 2 cut(s) 240, 818
Tsp45I GTSAC 4 cut(s) 17, 417, 452, 514
TspDTI ATGAA 4 cut(s) 212, 254, 282, 911
TspGWI ACGGA 2 cut(s) 111, 803
Vha464I CTTAAG 1 cut(s) 695
VpaK11BI GGWCC 1 cut(s) 793
XapI RAATTY 3 cut(s) 200, 271, 713
XceI RCATGY 3 cut(s) 92, 233, 294
XmiI GTMKAC 1 cut(s) 977
XmnI GAANNNNTTC 1 cut(s) 556
XspI CTAG 2 cut(s) 507, 951
ZrmI AGTACT 1 cut(s) 693
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.