FvH4_1g10460

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009509
Physical Location & Seq
Forward (+)
5715120 .. 5718239
3120 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10460.t2

Sequence Viewer

Length: 993 bp
ATGGATTCCTGTGACCCCCATTCTCTGAGATCTCTCTTAGTCTTCAAGAACACCATCAACCAAGAGTTGATTAGGAGCTTGCATGTGTATGGATTAGAGGATGGGAAGGAAAATGAGGTTGAGAGGGAGTTTCTGTTCTCTGATAACGGCTCATATGTGGAGCTTCGGGCTTACCCGGTTCTTAAATTGATGAAACTCAATGTTCCACAAGTGCTTGAAGGCTATGTTAATGGTTGCTGGGTCTGCATTCTAGCTTTTCATGCCAATCGTTCTCCTAACTTTACATGCATTCCTTGTATACTCTCTGTTTCCAGAAATCCTCAGTTAAAGCTGATCCCAAATTTAGCTAAGGATCTTCAAATGGTTGGCGAGATGACCTGCATAAAAGATGAGAAAGAACCACTGCAGAAGTCGGATGAGGAAACAAGCCAAGGGAATGATGGGAATTACTGTCAACTAAGAACAAGTTTACCTATGCTTGATCTGGATCTTAATCGCTCTCCTTGTTCTGTCACAGTGTCTGAATCATCTGATGATCAGGAAAGTGGAAGACAATCACCAGGTAATATAGAGAAGAAGAATAGAAGGGCAGCAAGTGAACGCATATCAAAAATCACTTTACCAGATCTGGTGAAGTATTTTGATATCCCTATTGTAGAAGCTTCAAGAAGTCTGAATGTTGGACTTACAGTGCTGAAAAAGAAATGTAGAGAGTTTGGTATTCCTCGGTGGCCACATAGGAAGATAAAATCGCTTGACAGCCTCATCCGTGATCTTCAGGAAGAGACGGAGTTCCAACAGAAGGAGAACAAGGATGCAGCCTTAGCAGTTGCAAAGAGGCAAAGGATGTTGGAGCGTGAAAGAGAAAGCATAGAGAGGAAACCCTTCTTGGATATGAAGATCGAGACCAAAAGGTTCAGGCAAGATGTTTTCAAGAGAAGGCATAGAGCCAGAGTTCTTAAAAGCCAAGGCCTATCACTCTCTGGCAACTAA

Protein Analysis

331

Amino Acids

37.95

Weight (kDa)

8.87

Isoelectric Point (pI)

53.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 113 - 161 2.6e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 386
AccI GTMKAC 1 cut(s) 298
AclWI GGATC 3 cut(s) 328, 360, 495
AcoI YGGCCR 1 cut(s) 731
AcsI RAATTY 1 cut(s) 340
AcuI CTGAAG 1 cut(s) 761
AfiI CCNNNNNNNGG 1 cut(s) 802
AgsI TTSAA 5 cut(s) 46, 218, 359, 666, 934
AjnI CCWGG 1 cut(s) 559
AjuI GAANNNNNNNTTGG 2 cut(s) 872, 904
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
AluBI AGCT 6 cut(s) 78, 163, 254, 331, 347, 662
AluI AGCT 6 cut(s) 78, 163, 254, 331, 347, 662
Alw26I GTCTC 2 cut(s) 779, 899
AlwI GGATC 3 cut(s) 328, 360, 495
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 2 cut(s) 731, 970
ApeKI GCWGC 2 cut(s) 590, 818
ApoI RAATTY 1 cut(s) 340
Asp700I GAANNNNTTC 1 cut(s) 884
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 2 cut(s) 549, 643
BalI TGGCCA 1 cut(s) 733
BbsI GAAGAC 2 cut(s) 34, 556
BbvI GCAGC 2 cut(s) 602, 830
BccI CCATC 3 cut(s) 62, 95, 434
BceAI ACGGC 1 cut(s) 163
BciT130I CCWGG 1 cut(s) 561
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 1 cut(s) 176
BcoDI GTCTC 2 cut(s) 779, 899
BfaI CTAG 1 cut(s) 251
BfmI CTRYAG 1 cut(s) 404
BfuAI ACCTGC 1 cut(s) 386
BglII AGATCT 2 cut(s) 29, 625
BisI GCNGC 2 cut(s) 591, 819
BlsI GCNGC 2 cut(s) 592, 820
Bme1390I CCNGG 2 cut(s) 176, 561
BmrFI CCNGG 2 cut(s) 176, 561
BmsI GCATC 1 cut(s) 805
BpiI GAAGAC 2 cut(s) 34, 556
Bpu10I CCTNAGC 2 cut(s) 348, 823
BpuMI CCSGG 1 cut(s) 176
BsaBI GATNNNNATC 2 cut(s) 486, 492
BsaI GGTCTC 1 cut(s) 899
BsaJI CCNNGG 3 cut(s) 430, 725, 967
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 1 cut(s) 802
Bse8I GATNNNNATC 2 cut(s) 486, 492
BseBI CCWGG 1 cut(s) 561
BseDI CCNNGG 3 cut(s) 430, 725, 967
BseGI GGATG 5 cut(s) 106, 421, 765, 820, 852
BseJI GATNNNNATC 2 cut(s) 486, 492
BseLI CCNNNNNNNGG 1 cut(s) 802
BseMII CTCAG 2 cut(s) 17, 335
BseXI GCAGC 2 cut(s) 602, 830
BseYI CCCAGC 1 cut(s) 237
BshFI GGCC 2 cut(s) 733, 972
BsiSI CCGG 1 cut(s) 176
BslI CCNNNNNNNGG 1 cut(s) 802
BsmAI GTCTC 2 cut(s) 779, 899
BsmBI CGTCTC 1 cut(s) 779
BsmI GAATGC 2 cut(s) 246, 288
BsnI GGCC 2 cut(s) 733, 972
Bso31I GGTCTC 1 cut(s) 899
Bsp143I GATC 9 cut(s) 29, 333, 352, 481, 487, 535, 625, 772, 900
BspANI GGCC 2 cut(s) 733, 972
BspCNI CTCAG 2 cut(s) 18, 334
BspMAI CTGCAG 1 cut(s) 408
BspMI ACCTGC 1 cut(s) 386
BspPI GGATC 3 cut(s) 328, 360, 495
BspTNI GGTCTC 1 cut(s) 899
BssECI CCNNGG 3 cut(s) 430, 725, 967
BssMI GATC 9 cut(s) 29, 333, 352, 481, 487, 535, 625, 772, 900
BssNAI GTATAC 1 cut(s) 299
BssT1I CCWWGG 2 cut(s) 430, 967
Bst1107I GTATAC 1 cut(s) 299
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 3 cut(s) 452, 517, 691
Bst6I CTCTTC 1 cut(s) 777
BstC8I GCNNGC 1 cut(s) 80
BstDEI CTNAG 6 cut(s) 26, 37, 321, 348, 458, 823
BstF5I GGATG 5 cut(s) 106, 421, 765, 820, 852
BstKTI GATC 9 cut(s) 32, 336, 355, 484, 490, 538, 628, 775, 903
BstMAI GTCTC 2 cut(s) 779, 899
BstMBI GATC 9 cut(s) 29, 333, 352, 481, 487, 535, 625, 772, 900
BstMWI GCNNNNNNNGC 3 cut(s) 243, 260, 824
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 2 cut(s) 86, 288
BstSCI CCNGG 2 cut(s) 174, 559
BstSFI CTRYAG 1 cut(s) 404
BstV1I GCAGC 2 cut(s) 602, 830
BstV2I GAAGAC 2 cut(s) 34, 556
BstX2I RGATCY 4 cut(s) 29, 352, 487, 625
BstYI RGATCY 4 cut(s) 29, 352, 487, 625
BstZ17I GTATAC 1 cut(s) 299
BsuRI GGCC 2 cut(s) 733, 972
BtsCI GGATG 5 cut(s) 106, 421, 765, 820, 852
BtsI GCAGTG 1 cut(s) 401
BtsIMutI CAGTG 3 cut(s) 401, 522, 696
BveI ACCTGC 1 cut(s) 386
Cac8I GCNNGC 1 cut(s) 80
CaiI CAGNNNCTG 1 cut(s) 521
CsiI ACCWGGT 1 cut(s) 559
CviAII CATG 3 cut(s) 83, 260, 285
DdeI CTNAG 6 cut(s) 26, 37, 321, 348, 458, 823
DpnI GATC 9 cut(s) 31, 335, 354, 483, 489, 537, 627, 774, 902
DpnII GATC 9 cut(s) 29, 333, 352, 481, 487, 535, 625, 772, 900
EaeI YGGCCR 1 cut(s) 731
Eam1104I CTCTTC 1 cut(s) 777
EarI CTCTTC 1 cut(s) 777
Eco130I CCWWGG 2 cut(s) 430, 967
Eco147I AGGCCT 1 cut(s) 972
Eco31I GGTCTC 1 cut(s) 899
Eco32I GATATC 1 cut(s) 646
Eco57I CTGAAG 1 cut(s) 761
EcoRII CCWGG 1 cut(s) 559
EcoRV GATATC 1 cut(s) 646
EcoT14I CCWWGG 2 cut(s) 430, 967
EcoT22I ATGCAT 1 cut(s) 290
ErhI CCWWGG 2 cut(s) 430, 967
Esp3I CGTCTC 1 cut(s) 779
FaeI CATG 3 cut(s) 86, 263, 288
FatI CATG 3 cut(s) 82, 259, 284
FauNDI CATATG 1 cut(s) 154
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 1 cut(s) 298
Fnu4HI GCNGC 2 cut(s) 591, 819
FokI GGATG 5 cut(s) 113, 428, 752, 827, 859
Fsp4HI GCNGC 2 cut(s) 591, 819
FspBI CTAG 1 cut(s) 251
GluI GCNGC 2 cut(s) 591, 819
GsaI CCCAGC 1 cut(s) 241
HaeIII GGCC 2 cut(s) 733, 972
HapII CCGG 1 cut(s) 176
Hin1II CATG 3 cut(s) 86, 263, 288
HincII GTYRAC 1 cut(s) 455
HindII GTYRAC 1 cut(s) 455
HindIII AAGCTT 1 cut(s) 660
HinfI GANTC 2 cut(s) 5, 524
HpaII CCGG 1 cut(s) 176
HphI GGTGA 2 cut(s) 549, 643
Hpy166II GTNNAC 4 cut(s) 299, 455, 470, 599
Hpy188I TCNGA 6 cut(s) 27, 142, 415, 523, 532, 675
Hpy188III TCNNGA 8 cut(s) 46, 312, 485, 539, 666, 779, 904, 934
Hpy8I GTNNAC 4 cut(s) 299, 455, 470, 599
HpyAV CCTTC 6 cut(s) 100, 212, 579, 796, 895, 933
HpyCH4III ACNGT 3 cut(s) 452, 517, 691
HpyCH4V TGCA 7 cut(s) 82, 246, 288, 381, 406, 818, 833
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 260, 824
HpyF3I CTNAG 6 cut(s) 26, 37, 321, 348, 458, 823
Hsp92II CATG 3 cut(s) 86, 263, 288
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 9 cut(s) 29, 333, 352, 481, 487, 535, 625, 772, 900
LmnI GCTCC 3 cut(s) 75, 160, 853
Lsp1109I GCAGC 2 cut(s) 602, 830
LweI GCATC 1 cut(s) 805
MabI ACCWGGT 1 cut(s) 559
MaeI CTAG 1 cut(s) 251
MaeIII GTNAC 2 cut(s) 11, 511
MalI GATC 9 cut(s) 31, 335, 354, 483, 489, 537, 627, 774, 902
MboI GATC 9 cut(s) 29, 333, 352, 481, 487, 535, 625, 772, 900
MboII GAAGA 9 cut(s) 34, 347, 561, 586, 589, 754, 767, 794, 910
MflI RGATCY 4 cut(s) 29, 352, 487, 625
MlsI TGGCCA 1 cut(s) 733
MluCI AATT 3 cut(s) 185, 340, 445
MluNI TGGCCA 1 cut(s) 733
MmeI TCCRAC 4 cut(s) 393, 661, 820, 831
MnlI CCTC 9 cut(s) 91, 109, 117, 330, 412, 735, 773, 831, 870
Mox20I TGGCCA 1 cut(s) 733
Mph1103I ATGCAT 1 cut(s) 290
MroXI GAANNNNTTC 1 cut(s) 884
MscI TGGCCA 1 cut(s) 733
MseI TTAA 5 cut(s) 183, 228, 326, 492, 960
MslI CAYNNNNRTG 1 cut(s) 87
Msp20I TGGCCA 1 cut(s) 733
MspI CCGG 1 cut(s) 176
MspR9I CCNGG 2 cut(s) 176, 561
Mva1269I GAATGC 2 cut(s) 246, 288
MvaI CCWGG 1 cut(s) 561
MwoI GCNNNNNNNGC 3 cut(s) 243, 260, 824
NciI CCSGG 1 cut(s) 176
NdeI CATATG 1 cut(s) 154
NdeII GATC 9 cut(s) 29, 333, 352, 481, 487, 535, 625, 772, 900
NlaIII CATG 3 cut(s) 86, 263, 288
NmuCI GTSAC 2 cut(s) 11, 511
NsiI ATGCAT 1 cut(s) 290
NspI RCATGY 2 cut(s) 86, 288
PceI AGGCCT 1 cut(s) 972
PctI GAATGC 2 cut(s) 246, 288
PdmI GAANNNNTTC 1 cut(s) 884
PfeI GAWTC 2 cut(s) 5, 524
PkrI GCNGC 2 cut(s) 592, 820
Psp6I CCWGG 1 cut(s) 559
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 1 cut(s) 559
PstI CTGCAG 1 cut(s) 408
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 4 cut(s) 29, 352, 487, 625
RseI CAYNNNNRTG 1 cut(s) 87
SaqAI TTAA 5 cut(s) 183, 228, 326, 492, 960
SatI GCNGC 2 cut(s) 591, 819
Sau3AI GATC 9 cut(s) 29, 333, 352, 481, 487, 535, 625, 772, 900
ScrFI CCNGG 2 cut(s) 176, 561
SexAI ACCWGGT 1 cut(s) 559
SfaNI GCATC 1 cut(s) 805
SfcI CTRYAG 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 87
Sse9I AATT 3 cut(s) 185, 340, 445
SseBI AGGCCT 1 cut(s) 972
SspMI CTAG 1 cut(s) 251
StuI AGGCCT 1 cut(s) 972
StyD4I CCNGG 2 cut(s) 174, 559
StyI CCWWGG 2 cut(s) 430, 967
TaaI ACNGT 3 cut(s) 452, 517, 691
TaqI TCGA 1 cut(s) 903
TasI AATT 3 cut(s) 185, 340, 445
TfiI GAWTC 2 cut(s) 5, 524
Tru1I TTAA 5 cut(s) 183, 228, 326, 492, 960
Tru9I TTAA 5 cut(s) 183, 228, 326, 492, 960
TscAI CASTG 3 cut(s) 408, 522, 696
TseFI GTSAC 2 cut(s) 11, 511
TseI GCWGC 2 cut(s) 590, 818
Tsp45I GTSAC 2 cut(s) 11, 511
TspDTI ATGAA 3 cut(s) 206, 248, 911
TspGWI ACGGA 2 cut(s) 758, 803
TspRI CASTG 3 cut(s) 408, 522, 696
XapI RAATTY 1 cut(s) 340
XceI RCATGY 2 cut(s) 86, 288
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 884
XspI CTAG 1 cut(s) 251
Zsp2I ATGCAT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.