Rroxscaffold_2G00144780

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009509
Physical Location & Seq
Reverse (-)
82678155 .. 82680792
2638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144780.1

Sequence Viewer

Length: 993 bp
ATGGATTCCTGTGACCCCCATTTTCTGAGATCTCTCCTAGTCTTCAAGAACACCAACAACCAAGAGTTGATTAGGAGCTTGCATGTGTATGGATTAGAGGATGGGAAGGAAAATGAGGTTGAGAGGGAGTTTCTGTTCTCAGAAAACGGGTCATATGTGGAGTTTCGGGCTTACCCGGTTCTTAGATTGATGAAATTCCGGGTTTCACAAGTGCTTGAAGGCTATGTTAATGGCTGCTGGGTCTGCATTTTAGCTTTTCATGCCAATCGTTCTCCTAATTTCACATGCATTCCTTCTATACTCTCTGTTTCAAGAAATCCTCAGTTAAAGTTGATCCCAAATTTAGCTAATGATCTTCAAATGGTTGTCGAGACAACCTGCATAAAAGCTGAGAAAGAACCATTGCAGAAGTCAGATGAGGAAACAAGCCAAGGGAATGATGAGAATCACTGTCAACTAAGAAGAAGTTTGCCTATGCTTGATCTGGATCTTAATCGTCCTCCTTGTCCTGCCACAATGTCTGAATCATTTGATGATCAAGAAAGTGGAAGACAATCACCCGGTTTTATAGAGAAGAAGAATAGAAGAGCGGCAAGTGACCGCATAGCAAAGATCACTTTACCAGATCTGGTGAAGTATTTTGATATCCCTATTGTGGAAGCTTCAAGAAGTCTGAATGTTGGACTTACAGTACTGAAAAAGAAATGTAGAGAGTTTGGTATTCCTCGTTGGCCACATAGGAAGATAAAATCGCTTGACAGCCTCATTCGTGATCTTCAGGAAGAGACGGAGTTCCAACAGAAGGAGAACAAGGCTGCAGCCTTAGCAGTTGCAAAGAGGCAAAGGATGTTGGAGAGTGAAAAAGAAAGCATAGAGAGGAGACCCTTCTTGGATATGAAGATCGAGACCAAGAGGTTCAGGCAAGATGTTTTCAAGAGAAGGCATAGAGCCAGAGTTCTTAGAAGCCAGGGCCTATCACTCTCTGGCAATTAA

Protein Analysis

330

Amino Acids

38.2

Weight (kDa)

9.04

Isoelectric Point (pI)

63.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 204 - 252 4.3e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 386
AccBSI CCGCTC 1 cut(s) 590
AciI CCGC 2 cut(s) 590, 601
AclWI GGATC 2 cut(s) 328, 495
AcoI YGGCCR 1 cut(s) 731
AcsI RAATTY 2 cut(s) 194, 340
AcuI CTGAAG 1 cut(s) 761
AfaI GTAC 1 cut(s) 693
AfiI CCNNNNNNNGG 2 cut(s) 655, 802
AgsI TTSAA 6 cut(s) 46, 218, 312, 359, 666, 934
AjnI CCWGG 1 cut(s) 966
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
AluBI AGCT 5 cut(s) 78, 254, 347, 389, 662
AluI AGCT 5 cut(s) 78, 254, 347, 389, 662
Alw26I GTCTC 4 cut(s) 365, 779, 874, 899
AlwI GGATC 2 cut(s) 328, 495
AoxI GGCC 2 cut(s) 731, 970
ApeKI GCWGC 3 cut(s) 234, 815, 818
ApoI RAATTY 2 cut(s) 194, 340
AspS9I GGNCC 1 cut(s) 970
AsuC2I CCSGG 3 cut(s) 176, 200, 561
AsuHPI GGTGA 2 cut(s) 549, 643
BalI TGGCCA 1 cut(s) 733
BbsI GAAGAC 2 cut(s) 34, 556
BbvI GCAGC 3 cut(s) 221, 802, 830
BccI CCATC 1 cut(s) 95
BciT130I CCWGG 1 cut(s) 968
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 3 cut(s) 176, 200, 561
BcoDI GTCTC 4 cut(s) 365, 779, 874, 899
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 816
BfuAI ACCTGC 1 cut(s) 386
BglII AGATCT 2 cut(s) 29, 625
BisI GCNGC 4 cut(s) 235, 591, 816, 819
BlsI GCNGC 4 cut(s) 236, 592, 817, 820
BmcAI AGTACT 1 cut(s) 693
Bme1390I CCNGG 4 cut(s) 176, 200, 561, 968
BmgT120I GGNCC 1 cut(s) 970
BmrFI CCNGG 4 cut(s) 176, 200, 561, 968
BpiI GAAGAC 2 cut(s) 34, 556
Bpu10I CCTNAGC 1 cut(s) 823
BpuMI CCSGG 3 cut(s) 176, 200, 561
BsaBI GATNNNNATC 3 cut(s) 444, 486, 492
BsaI GGTCTC 2 cut(s) 874, 899
BsaJI CCNNGG 2 cut(s) 430, 967
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 2 cut(s) 655, 802
Bse3DI GCAATG 1 cut(s) 401
Bse8I GATNNNNATC 3 cut(s) 444, 486, 492
BseBI CCWGG 1 cut(s) 968
BseDI CCNNGG 2 cut(s) 430, 967
BseGI GGATG 2 cut(s) 106, 852
BseJI GATNNNNATC 3 cut(s) 444, 486, 492
BseLI CCNNNNNNNGG 2 cut(s) 655, 802
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 4 cut(s) 17, 153, 335, 381
BseRI GAGGAG 1 cut(s) 892
BseXI GCAGC 3 cut(s) 221, 802, 830
BseYI CCCAGC 1 cut(s) 237
BshFI GGCC 2 cut(s) 733, 972
BsiSI CCGG 3 cut(s) 176, 199, 561
BslI CCNNNNNNNGG 2 cut(s) 655, 802
BsmAI GTCTC 4 cut(s) 365, 779, 874, 899
BsmBI CGTCTC 1 cut(s) 779
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 2 cut(s) 733, 972
Bso31I GGTCTC 2 cut(s) 874, 899
BspACI CCGC 2 cut(s) 590, 601
BspANI GGCC 2 cut(s) 733, 972
BspCNI CTCAG 4 cut(s) 18, 152, 334, 382
BspMAI CTGCAG 1 cut(s) 820
BspMI ACCTGC 1 cut(s) 386
BspPI GGATC 2 cut(s) 328, 495
BspQI GCTCTTC 1 cut(s) 580
BspTNI GGTCTC 2 cut(s) 874, 899
BsrBI CCGCTC 1 cut(s) 590
BsrDI GCAATG 1 cut(s) 401
BssECI CCNNGG 2 cut(s) 430, 967
BssT1I CCWWGG 1 cut(s) 430
Bst2UI CCWGG 1 cut(s) 968
Bst4CI ACNGT 2 cut(s) 452, 691
Bst6I CTCTTC 2 cut(s) 580, 777
BstC8I GCNNGC 1 cut(s) 80
BstDEI CTNAG 8 cut(s) 26, 139, 182, 321, 390, 458, 823, 959
BstF5I GGATG 2 cut(s) 106, 852
BstMAI GTCTC 4 cut(s) 365, 779, 874, 899
BstMWI GCNNNNNNNGC 3 cut(s) 243, 260, 824
BstNI CCWGG 1 cut(s) 968
BstNSI RCATGY 2 cut(s) 86, 288
BstSCI CCNGG 4 cut(s) 174, 198, 559, 966
BstSFI CTRYAG 1 cut(s) 816
BstV1I GCAGC 3 cut(s) 221, 802, 830
BstV2I GAAGAC 2 cut(s) 34, 556
BstX2I RGATCY 3 cut(s) 29, 487, 625
BstYI RGATCY 3 cut(s) 29, 487, 625
BsuRI GGCC 2 cut(s) 733, 972
BtsCI GGATG 2 cut(s) 106, 852
BtsIMutI CAGTG 1 cut(s) 448
BveI ACCTGC 1 cut(s) 386
Cac8I GCNNGC 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 970
Csp6I GTAC 1 cut(s) 692
CviAII CATG 3 cut(s) 83, 260, 285
CviQI GTAC 1 cut(s) 692
DdeI CTNAG 8 cut(s) 26, 139, 182, 321, 390, 458, 823, 959
EaeI YGGCCR 1 cut(s) 731
Eam1104I CTCTTC 2 cut(s) 580, 777
EarI CTCTTC 2 cut(s) 580, 777
Eco130I CCWWGG 1 cut(s) 430
Eco31I GGTCTC 2 cut(s) 874, 899
Eco32I GATATC 1 cut(s) 646
Eco57I CTGAAG 1 cut(s) 761
EcoO109I RGGNCCY 1 cut(s) 970
EcoRII CCWGG 1 cut(s) 966
EcoRV GATATC 1 cut(s) 646
EcoT14I CCWWGG 1 cut(s) 430
EcoT22I ATGCAT 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 430
Esp3I CGTCTC 1 cut(s) 779
FaeI CATG 3 cut(s) 86, 263, 288
FatI CATG 3 cut(s) 82, 259, 284
FauNDI CATATG 1 cut(s) 154
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 4 cut(s) 235, 591, 816, 819
FokI GGATG 2 cut(s) 113, 859
Fsp4HI GCNGC 4 cut(s) 235, 591, 816, 819
FspBI CTAG 1 cut(s) 38
GluI GCNGC 4 cut(s) 235, 591, 816, 819
GsaI CCCAGC 1 cut(s) 241
HaeIII GGCC 2 cut(s) 733, 972
HapII CCGG 3 cut(s) 176, 199, 561
Hin1II CATG 3 cut(s) 86, 263, 288
HincII GTYRAC 1 cut(s) 455
HindII GTYRAC 1 cut(s) 455
HindIII AAGCTT 1 cut(s) 660
HinfI GANTC 3 cut(s) 5, 445, 524
HpaII CCGG 3 cut(s) 176, 199, 561
HphI GGTGA 2 cut(s) 549, 643
Hpy166II GTNNAC 1 cut(s) 455
Hpy188I TCNGA 5 cut(s) 27, 142, 415, 523, 675
Hpy8I GTNNAC 1 cut(s) 455
HpyAV CCTTC 6 cut(s) 100, 212, 303, 796, 895, 933
HpyCH4III ACNGT 2 cut(s) 452, 691
HpyCH4V TGCA 7 cut(s) 82, 246, 288, 381, 406, 818, 833
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 260, 824
HpyF3I CTNAG 8 cut(s) 26, 139, 182, 321, 390, 458, 823, 959
Hsp92II CATG 3 cut(s) 86, 263, 288
Ksp22I TGATCA 1 cut(s) 535
LguI GCTCTTC 1 cut(s) 580
LmnI GCTCC 1 cut(s) 75
Lsp1109I GCAGC 3 cut(s) 221, 802, 830
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 2 cut(s) 11, 596
MbiI CCGCTC 1 cut(s) 590
MflI RGATCY 3 cut(s) 29, 487, 625
MlsI TGGCCA 1 cut(s) 733
MluCI AATT 4 cut(s) 194, 277, 340, 988
MluNI TGGCCA 1 cut(s) 733
MmeI TCCRAC 3 cut(s) 661, 820, 831
Mox20I TGGCCA 1 cut(s) 733
Mph1103I ATGCAT 1 cut(s) 290
MscI TGGCCA 1 cut(s) 733
MseI TTAA 4 cut(s) 228, 326, 492, 991
MslI CAYNNNNRTG 1 cut(s) 87
Msp20I TGGCCA 1 cut(s) 733
MspI CCGG 3 cut(s) 176, 199, 561
MspR9I CCNGG 4 cut(s) 176, 200, 561, 968
Mva1269I GAATGC 1 cut(s) 288
MvaI CCWGG 1 cut(s) 968
MwoI GCNNNNNNNGC 3 cut(s) 243, 260, 824
NciI CCSGG 3 cut(s) 176, 200, 561
NdeI CATATG 1 cut(s) 154
NlaIII CATG 3 cut(s) 86, 263, 288
NmuCI GTSAC 2 cut(s) 11, 596
NsiI ATGCAT 1 cut(s) 290
NspI RCATGY 2 cut(s) 86, 288
PciSI GCTCTTC 1 cut(s) 580
PctI GAATGC 1 cut(s) 288
PfeI GAWTC 3 cut(s) 5, 445, 524
PkrI GCNGC 4 cut(s) 236, 592, 817, 820
Psp6I CCWGG 1 cut(s) 966
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 1 cut(s) 966
PspPI GGNCC 1 cut(s) 970
PstI CTGCAG 1 cut(s) 820
PsuI RGATCY 3 cut(s) 29, 487, 625
RsaI GTAC 1 cut(s) 693
RsaNI GTAC 1 cut(s) 692
RseI CAYNNNNRTG 1 cut(s) 87
SapI GCTCTTC 1 cut(s) 580
SaqAI TTAA 4 cut(s) 228, 326, 492, 991
SatI GCNGC 4 cut(s) 235, 591, 816, 819
Sau96I GGNCC 1 cut(s) 970
ScaI AGTACT 1 cut(s) 693
ScrFI CCNGG 4 cut(s) 176, 200, 561, 968
SetI ASST 8 cut(s) 80, 120, 256, 349, 380, 391, 664, 917
SfcI CTRYAG 1 cut(s) 816
SmiMI CAYNNNNRTG 1 cut(s) 87
Sse9I AATT 4 cut(s) 194, 277, 340, 988
SsiI CCGC 2 cut(s) 590, 601
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 4 cut(s) 174, 198, 559, 966
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 2 cut(s) 452, 691
TaqI TCGA 2 cut(s) 369, 903
TasI AATT 4 cut(s) 194, 277, 340, 988
TatI WGTACW 1 cut(s) 691
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 3 cut(s) 5, 445, 524
Tru1I TTAA 4 cut(s) 228, 326, 492, 991
Tru9I TTAA 4 cut(s) 228, 326, 492, 991
TscAI CASTG 1 cut(s) 455
TseFI GTSAC 2 cut(s) 11, 596
TseI GCWGC 3 cut(s) 234, 815, 818
Tsp45I GTSAC 2 cut(s) 11, 596
TspDTI ATGAA 3 cut(s) 206, 248, 911
TspGWI ACGGA 1 cut(s) 803
TspRI CASTG 1 cut(s) 455
XapI RAATTY 2 cut(s) 194, 340
XceI RCATGY 2 cut(s) 86, 288
XspI CTAG 1 cut(s) 38
ZrmI AGTACT 1 cut(s) 693
Zsp2I ATGCAT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.