Rorug02G0065800

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009509
Physical Location & Seq
Forward (+)
5148697 .. 5151586
2890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0065800.1

Sequence Viewer

Length: 2700 bp
ATGATTAGAAAACCGACGGGCACGTTCAAGCACCAACTCTTGTTCTCCTCCTCCTCCTCCTCCTCCTACAGCAGCTGTAGGAGGAGGAGGATTCCAGCACCAGCACCAGCGGACAACCTCCCTTGCCAGCTCTTCGCCATCCTCTGCGGCCCAAACTGGCAAAAACACCCCTCCCTCAAAGCCCTTATCCCCTCCATATCCCCCTCCCACGTCTCCTCCCTCTTCTCCCTCAACCCGGTCCACCCCCGAACCGCCCTCCACTTCTTCACCTGGCTCGCCCACAACCACCCCAAATTCCACCACAACGTCCACTCCCACTCCTCCCTCCTCGCCCTCCTCCTCCGCAACGGCTTCTCCTCCGGCCCCGCCGAGAGGGTCCGCATTTCCATGATTAAGGCTTCAATTTCTAACGCCGACGCCCGGTTCGTGCTGGACCGGTTGCGCCGGTTCAAGCACGAATTCGGGTTCAACATCACTCTCAGGTGTTATGATTCTCTTTTATTCTCAGTTTCCAAGTTGTTAGGGATTCAAGATTTGAAAATGTTGTATTTGGAAATGCTGGAGGATAAAATCTCCCCTGATTTGCATATATATAATATCATGGTGAATGCTTATTGTAGATTAGGCAATGTAGTTGAGGCTGGTGTCTATGTTGGTAAGATCGTTGAGGCGGGTTTGAAGCCGAATACCAATACTTTCAATTCTTTGATAATGGGGCATTGTAGGAATAAGGATGTGGATAGTGCTTACAGGACTTTTAAGGCCATCCCCAACAAGGGTTGTGCAAGAGATGTGGTTTCCTACAATGTGTTGATACACGGGCTCTGTGAGGCTGGCAGGAGTCACGAGGCGTTTGAGTTGTACTCTAATATGGAGAAGGATGAGTGTTTACCAGATGCGCCCACGTATAATGTTCTTATTCATGCATTGTGTAGAGGGGGGAGGAGATTGGAAGGGATGAGTTTGTTTAAGGAGATGAAAGAGAAGGGTTGTGAGCCTAGTGTAAATACTTATAATGTGCTCATTACTAGCCTGTGTGACGAAAACAAGCTTCAGTATGCTAGCAAGGTTCTAGACCTGATGTCGGGGAAAGGGTTGGTTCCCGATGTTGTCACCTACAATGCATTGATTGATGGGTATTGCAAGGAGGGAGCGGTTGAGGCTGCGTTTGGGATTCTGGGTTTGATGGAATCTAACAACTGTCGTCCAAATGCTCGCACCTTTACTGAATTGATTCATGGGTTTTGTAAAAGGAATAATGTGCACCAGGCAATGGCGCTTCTTGATAGGATGCTTGAACGGAAGCTCTCACCAGACCGGATTACTTATAATTCATTGATCAATGGGCAGTGTAAAGCAGGTCATGTAGATAGTGCTTATAGGTTGCTTAGTTTGATGAAACATAGTGGTTTAGTTCCTGACCAGTGGACTTACAGTGTTATCATCGACAGTCTCTGTAAGTTGGGGAGATTAGAAGAAGCTCAAGTCCTCTTCGATTCTCTCAAGGACAAAGGCATCAAGTCTAATGAAGTGATATTTACTGCTTTACTTGATGGTTACTGCAAGGTTGGGAAAATCGATGATGCTCGCACTTTATTTAATAGAATGCTTGCCGAGGGTTGTTTCCCAAACTCATATACTTACTGTGCCTTCCTAGGTGGGCTGTGCAAAGAGGGAAAAACAAAGGAAGCATTATCAGTGGTGGAACAGATGCTAAGTAGTGGTGTGAAGCCTACAGTCCACACTTGTAACGTTCTAATTAACCATGTGCTGAAAGAAGGGGATTTCAACCATGCTCAGAGGTTTTTGAATGAATTTGTTTCTCTTGGTTACCGACCTGATGCAGTTATGTACGGTACATTTGTTCATGCATATTGCAGCATAGATAATATAAAAGAAGCAGAGAAGGTAATGACTAAGATGGATGAAGAAGGAATTTCTGCAGATTCATTGACATACGCATTTTTAATCGAGGCATATCTTCGTTTGGGATTAATAGATTCTGCATTTGATGTCTTTAAGCGCATGTTTGATGCTCATTGCAACCCTTGTCATCGCATCTATTACTTGCTCATCAAACACCTGTCAAATGAAAAGTTGCTGCAGACCGACGATAATGTAGTAGGACTTCATATGGTTTCAAGTGTCCCATCAGCTGATATGTGGAAGACAGTGGATTTTGAAATTGCTTTAGAGCTCCTTGAGAAGATGAATGAACATGGTTGTGCACCGAATGTGAAAACTTACGGCGAGCTTATAGTAGCACTTTGCAAAGTTAGGAACTTGGAAGTAGCCCGTAAGTTGTATGCTCATATGAGAGATACCAGCATTTCTCCTAGTGAGGATATTTGTAACTCTCTTCTTAAATGTTGCTGCGAGTTGAAAGTGTATGGAGAAGCAGCTATTCTGGTGGACGCTATGATTCAGCTTGGTTATTTACCAGCATTGGAGTCGTGCAAATTGCTTGTATGTGGACTTTTTGAAGAAGAGAATAACGAGAAGGCAAATTCTGTTTTCTGCAGAATTCTTTGTTGCGCGTATAACTACGATGAAGTAGCCTGGAAAGTTCTTCTTGAAGGTTTACTTAAGAGGGGTCATATCAATAGATGCTCTGAGATGGTAACCGTCATCGAAAATATGGGTTACAAGCTTCATCCTGAGACCTATTCAATGTTAATTGAGGGATTTGATGGAATATAA

Protein Analysis

899

Amino Acids

100.94

Weight (kDa)

7.31

Isoelectric Point (pI)

32.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 181 - 290 2.1e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 193 - 242 8.5e-09 PPR repeat family
PPR_3 PF13812 220 - 273 3.6e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 225 - 253 2.7e-08 PPR repeat
PPR_2 PF13041 228 - 276 4.6e-13 PPR repeat family
PPR_long PF17177 242 - 379 1.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 259 - 309 2.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 259 - 292 4.1e-11 PPR repeat
PPR_2 PF13041 263 - 312 3.6e-18 PPR repeat family
PPR PF01535 266 - 296 5.9e-07 PPR repeat
PPR_3 PF13812 287 - 343 2.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 295 - 327 2.1e-07 PPR repeat
PPR_2 PF13041 302 - 347 4.2e-15 PPR repeat family
PPR PF01535 302 - 331 4.7e-08 PPR repeat
PPR_long PF17177 320 - 481 1.8e-13 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 321 - 380 6.1e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 329 - 361 3.8e-08 PPR repeat
PPR_2 PF13041 335 - 374 1.5e-08 PPR repeat family
TPR_24 PF23276 349 - 432 1e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 357 - 408 2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 364 - 397 4.4e-12 PPR repeat
PPR_2 PF13041 368 - 417 6.1e-18 PPR repeat family
PPR PF01535 371 - 401 1.2e-07 PPR repeat
PPR_3 PF13812 395 - 448 4.6e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 400 - 431 4.5e-10 PPR repeat
PPR_2 PF13041 407 - 452 4.6e-13 PPR repeat family
PPR_1 PF12854 436 - 467 9.2e-12 PPR repeat
PPR_2 PF13041 438 - 487 1.5e-16 PPR repeat family
PPR PF01535 441 - 471 1.7e-06 PPR repeat
PPR_1 PF12854 470 - 500 1.2e-09 PPR repeat
PPR PF01535 477 - 506 2.8e-06 PPR repeat
PPR_long PF17177 493 - 606 3.7e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 497 - 553 3.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 504 - 536 5.9e-09 PPR repeat
PPR_2 PF13041 509 - 557 1.7e-15 PPR repeat family
PPR PF01535 511 - 541 2.3e-07 PPR repeat
PPR_1 PF12854 540 - 571 8e-09 PPR repeat
PPR_2 PF13041 551 - 589 3.8e-09 PPR repeat family
PPR_2 PF13041 578 - 626 2.2e-07 PPR repeat family
PPR_3 PF13812 603 - 660 1.9e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 610 - 641 9.3e-06 PPR repeat
PPR_2 PF13041 613 - 658 7.2e-08 PPR repeat family
PPR_long PF17177 723 - 793 1.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 733 - 791 1.4e-07 Pentatricopeptide repeat domain
PPR_long PF17177 765 - 894 4.2e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1014, 1329
Acc36I ACCTGC 1 cut(s) 1349
AccB7I CCANNNNNTGG 1 cut(s) 1273
AccBSI CCGCTC 1 cut(s) 1154
AccII CGCG 1 cut(s) 2537
AciI CCGC 8 cut(s) 110, 147, 252, 343, 366, 379, 671, 1154
AclI AACGTT 1 cut(s) 1752
AcsI RAATTY 6 cut(s) 293, 458, 1814, 1935, 2506, 2523
AcuI CTGAAG 1 cut(s) 1037
AcyI GRCGYC 1 cut(s) 417
AfaI GTAC 3 cut(s) 863, 1853, 1858
AfiI CCNNNNNNNGG 7 cut(s) 235, 372, 420, 775, 776, 1084, 1273
AflII CTTAAG 1 cut(s) 2585
AgeI ACCGGT 1 cut(s) 435
AhdI GACNNNNNGTC 1 cut(s) 1081
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 3 cut(s) 269, 1266, 2558
Alw21I GWGCWC 4 cut(s) 1023, 1266, 2199, 2230
Alw26I GTCTC 3 cut(s) 217, 1457, 2654
Alw44I GTGCAC 2 cut(s) 1262, 2226
AlwNI CAGNNNCTG 2 cut(s) 75, 1455
AoxI GGCC 3 cut(s) 148, 361, 762
ApaLI GTGCAC 2 cut(s) 1262, 2226
ApeKI GCWGC 6 cut(s) 72, 1163, 1878, 2101, 2373, 2399
ApoI RAATTY 6 cut(s) 293, 458, 1814, 1935, 2506, 2523
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AseI ATTAAT 1 cut(s) 1994
AsiGI ACCGGT 1 cut(s) 435
Asp700I GAANNNNTTC 1 cut(s) 1233
AspA2I CCTAGG 1 cut(s) 1654
AspLEI GCGC 5 cut(s) 444, 901, 1279, 2025, 2537
AspS9I GGNCC 5 cut(s) 149, 238, 362, 376, 433
AsuC2I CCSGG 2 cut(s) 236, 421
AsuHPI GGTGA 4 cut(s) 259, 616, 1105, 1302
AsuNHI GCTAGC 1 cut(s) 1061
AvaII GGWCC 3 cut(s) 238, 376, 433
AvrII CCTAGG 1 cut(s) 1654
BaeGI GKGCMC 3 cut(s) 23, 1266, 2230
BaeI ACNNNNGTAYC 2 cut(s) 1848, 1881
BanII GRGCYC 2 cut(s) 825, 2199
BarI GAAGNNNNNNTAC 2 cut(s) 2112, 2144
BauI CACGAG 1 cut(s) 845
BbsI GAAGAC 1 cut(s) 2174
Bbv12I GWGCWC 4 cut(s) 1023, 1266, 2199, 2230
BbvI GCAGC 6 cut(s) 84, 1150, 1890, 2088, 2360, 2411
BccI CCATC 9 cut(s) 146, 773, 1127, 1180, 1547, 1915, 2158, 2611, 2684
BceAI ACGGC 2 cut(s) 364, 2263
BcgI CGANNNNNNTGC 2 cut(s) 2433, 2467
BciT130I CCWGG 3 cut(s) 271, 1268, 2560
BclI TGATCA 1 cut(s) 1338
BcnI CCSGG 2 cut(s) 236, 421
BcoDI GTCTC 3 cut(s) 217, 1457, 2654
BfaI CTAG 6 cut(s) 999, 1029, 1062, 1073, 1655, 2337
BfmI CTRYAG 6 cut(s) 67, 76, 1734, 1941, 2102, 2518
BfoI RGCGCY 1 cut(s) 1280
BfrI CTTAAG 1 cut(s) 2585
BfuAI ACCTGC 1 cut(s) 1349
BisI GCNGC 7 cut(s) 73, 148, 1164, 1879, 2102, 2374, 2400
BlnI CCTAGG 1 cut(s) 1654
BlsI GCNGC 7 cut(s) 74, 149, 1165, 1880, 2103, 2375, 2401
Bme1390I CCNGG 5 cut(s) 236, 271, 421, 1268, 2560
Bme18I GGWCC 3 cut(s) 238, 376, 433
BmeRI GACNNNNNGTC 1 cut(s) 1081
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 5 cut(s) 149, 238, 362, 376, 433
BmiI GGNNCC 3 cut(s) 364, 377, 1101
BmrFI CCNGG 5 cut(s) 236, 271, 421, 1268, 2560
BmsI GCATC 9 cut(s) 886, 1281, 1524, 1573, 1701, 1831, 2023, 2067, 2597
BmtI GCTAGC 1 cut(s) 1065
BpiI GAAGAC 1 cut(s) 2174
BplI GAGNNNNNCTC 2 cut(s) 848, 880
BpmI CTGGAG 1 cut(s) 581
BpuEI CTTGAG 3 cut(s) 1467, 1487, 2222
BpuMI CCSGG 2 cut(s) 236, 421
Bsa29I ATCGAT 1 cut(s) 1578
BsaAI YACGTR 1 cut(s) 906
BsaHI GRCGYC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 2654
BsaJI CCNNGG 2 cut(s) 1614, 1654
BsaWI WCCGGW 2 cut(s) 435, 1317
BsaXI ACNNNNNCTCC 8 cut(s) 200, 230, 296, 302, 326, 332, 338, 368
Bsc4I CCNNNNNNNGG 7 cut(s) 235, 372, 420, 775, 776, 1084, 1273
Bse118I RCCGGY 2 cut(s) 435, 444
Bse1I ACTGG 2 cut(s) 161, 1423
Bse3DI GCAATG 3 cut(s) 634, 1278, 2038
BseBI CCWGG 3 cut(s) 271, 1268, 2560
BseCI ATCGAT 1 cut(s) 1578
BseDI CCNNGG 2 cut(s) 1614, 1654
BseGI GGATG 8 cut(s) 138, 739, 765, 886, 963, 1296, 1930, 2653
BseLI CCNNNNNNNGG 7 cut(s) 235, 372, 420, 775, 776, 1084, 1273
BseMI GCAATG 3 cut(s) 634, 1278, 2038
BseMII CTCAG 5 cut(s) 493, 519, 1811, 2604, 2649
BseNI ACTGG 2 cut(s) 161, 1423
BseSI GKGCMC 3 cut(s) 23, 1266, 2230
BseXI GCAGC 6 cut(s) 84, 1150, 1890, 2088, 2360, 2411
Bsh1236I CGCG 1 cut(s) 2537
BshFI GGCC 3 cut(s) 150, 363, 764
BshTI ACCGGT 1 cut(s) 435
BshVI ATCGAT 1 cut(s) 1578
BsiHKAI GWGCWC 4 cut(s) 1023, 1266, 2199, 2230
BsiSI CCGG 6 cut(s) 236, 360, 421, 436, 445, 1318
BslFI GGGAC 1 cut(s) 2132
BslI CCNNNNNNNGG 7 cut(s) 235, 372, 420, 775, 776, 1084, 1273
BsmAI GTCTC 3 cut(s) 217, 1457, 2654
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 2132
BsmI GAATGC 2 cut(s) 613, 1611
BsnI GGCC 3 cut(s) 150, 363, 764
Bso31I GGTCTC 1 cut(s) 2654
Bsp1286I GDGCHC 6 cut(s) 23, 825, 1023, 1266, 2199, 2230
Bsp143I GATC 2 cut(s) 660, 1338
BspACI CCGC 8 cut(s) 110, 147, 252, 343, 366, 379, 671, 1154
BspANI GGCC 3 cut(s) 150, 363, 764
BspCNI CTCAG 5 cut(s) 492, 518, 1810, 2605, 2650
BspDI ATCGAT 1 cut(s) 1578
BspFNI CGCG 1 cut(s) 2537
BspLI GGNNCC 3 cut(s) 364, 377, 1101
BspMAI CTGCAG 3 cut(s) 1945, 2106, 2522
BspMI ACCTGC 1 cut(s) 1349
BspOI GCTAGC 1 cut(s) 1065
BspQI GCTCTTC 1 cut(s) 137
BspTI CTTAAG 1 cut(s) 2585
BspTNI GGTCTC 1 cut(s) 2654
BsrBI CCGCTC 1 cut(s) 1154
BsrDI GCAATG 3 cut(s) 634, 1278, 2038
BsrFI RCCGGY 2 cut(s) 435, 444
BsrI ACTGG 2 cut(s) 161, 1423
BssAI RCCGGY 2 cut(s) 435, 444
BssECI CCNNGG 2 cut(s) 1614, 1654
BssMI GATC 2 cut(s) 660, 1338
BssNI GRCGYC 1 cut(s) 417
BssSI CACGAG 1 cut(s) 845
BssT1I CCWWGG 1 cut(s) 1654
Bst2BI CACGAG 1 cut(s) 845
Bst2UI CCWGG 3 cut(s) 271, 1268, 2560
Bst4CI ACNGT 8 cut(s) 1202, 1436, 1451, 1646, 1738, 1856, 2173, 2626
Bst6I CTCTTC 5 cut(s) 137, 227, 1496, 2364, 2481
BstACI GRCGYC 1 cut(s) 417
BstAFI CTTAAG 1 cut(s) 2585
BstBAI YACGTR 1 cut(s) 906
BstC8I GCNNGC 8 cut(s) 128, 276, 835, 1063, 1216, 1588, 1611, 2252
BstDEI CTNAG 8 cut(s) 479, 505, 1388, 1715, 1797, 1917, 2613, 2658
BstEII GGTNACC 2 cut(s) 1829, 2620
BstF5I GGATG 8 cut(s) 138, 739, 765, 886, 963, 1296, 1930, 2653
BstFNI CGCG 1 cut(s) 2537
BstH2I RGCGCY 1 cut(s) 1280
BstHHI GCGC 5 cut(s) 444, 901, 1279, 2025, 2537
BstKTI GATC 2 cut(s) 663, 1341
BstMAI GTCTC 3 cut(s) 217, 1457, 2654
BstMBI GATC 2 cut(s) 660, 1338
BstMWI GCNNNNNNNGC 1 cut(s) 1160
BstNI CCWGG 3 cut(s) 271, 1268, 2560
BstNSI RCATGY 1 cut(s) 2029
BstPI GGTNACC 2 cut(s) 1829, 2620
BstSCI CCNGG 5 cut(s) 234, 269, 419, 1266, 2558
BstSFI CTRYAG 6 cut(s) 67, 76, 1734, 1941, 2102, 2518
BstSLI GKGCMC 3 cut(s) 23, 1266, 2230
BstUI CGCG 1 cut(s) 2537
BstV1I GCAGC 6 cut(s) 84, 1150, 1890, 2088, 2360, 2411
BstV2I GAAGAC 1 cut(s) 2174
Bsu15I ATCGAT 1 cut(s) 1578
BsuRI GGCC 3 cut(s) 150, 363, 764
BsuTUI ATCGAT 1 cut(s) 1578
BtgZI GCGATG 1 cut(s) 2039
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 8 cut(s) 138, 739, 765, 886, 963, 1296, 1930, 2653
BtsI GCAGTG 1 cut(s) 1355
BtsIMutI CAGTG 5 cut(s) 1355, 1430, 1441, 1704, 2178
BveI ACCTGC 1 cut(s) 1349
Cac8I GCNNGC 8 cut(s) 128, 276, 835, 1063, 1216, 1588, 1611, 2252
CaiI CAGNNNCTG 2 cut(s) 75, 1455
CfoI GCGC 5 cut(s) 444, 901, 1279, 2025, 2537
Cfr10I RCCGGY 2 cut(s) 435, 444
Cfr13I GGNCC 5 cut(s) 149, 238, 362, 376, 433
ClaI ATCGAT 1 cut(s) 1578
CseI GACGC 2 cut(s) 425, 2423
Csp6I GTAC 3 cut(s) 862, 1852, 1857
CspAI ACCGGT 1 cut(s) 435
CspCI CAANNNNNGTGG 2 cut(s) 774, 809
CviQI GTAC 3 cut(s) 862, 1852, 1857
DdeI CTNAG 8 cut(s) 479, 505, 1388, 1715, 1797, 1917, 2613, 2658
DpnI GATC 2 cut(s) 662, 1340
DpnII GATC 2 cut(s) 660, 1338
DriI GACNNNNNGTC 1 cut(s) 1081
Eam1104I CTCTTC 5 cut(s) 137, 227, 1496, 2364, 2481
Eam1105I GACNNNNNGTC 1 cut(s) 1081
EarI CTCTTC 5 cut(s) 137, 227, 1496, 2364, 2481
Ecl136II GAGCTC 1 cut(s) 2197
Eco130I CCWWGG 1 cut(s) 1654
Eco24I GRGCYC 2 cut(s) 825, 2199
Eco31I GGTCTC 1 cut(s) 2654
Eco47I GGWCC 3 cut(s) 238, 376, 433
Eco53kI GAGCTC 1 cut(s) 2197
Eco57I CTGAAG 1 cut(s) 1037
Eco91I GGTNACC 2 cut(s) 1829, 2620
EcoICRI GAGCTC 1 cut(s) 2197
EcoO65I GGTNACC 2 cut(s) 1829, 2620
EcoRI GAATTC 2 cut(s) 458, 2523
EcoRII CCWGG 3 cut(s) 269, 1266, 2558
EcoT14I CCWWGG 1 cut(s) 1654
EcoT22I ATGCAT 3 cut(s) 928, 1126, 1873
EcoT38I GRGCYC 2 cut(s) 825, 2199
ErhI CCWWGG 1 cut(s) 1654
Esp3I CGTCTC 1 cut(s) 217
FalI AAGNNNNNCTT 4 cut(s) 2556, 2588, 2568, 2600
FaqI GGGAC 1 cut(s) 2132
FauI CCCGC 2 cut(s) 373, 664
FauNDI CATATG 2 cut(s) 2133, 2313
FbaI TGATCA 1 cut(s) 1338
Fnu4HI GCNGC 7 cut(s) 73, 148, 1164, 1879, 2102, 2374, 2400
FokI GGATG 8 cut(s) 125, 746, 752, 893, 970, 1303, 1937, 2640
FriOI GRGCYC 2 cut(s) 825, 2199
Fsp4HI GCNGC 7 cut(s) 73, 148, 1164, 1879, 2102, 2374, 2400
FspBI CTAG 6 cut(s) 999, 1029, 1062, 1073, 1655, 2337
GlaI GCGC 5 cut(s) 443, 900, 1278, 2024, 2536
GluI GCNGC 7 cut(s) 73, 148, 1164, 1879, 2102, 2374, 2400
GsuI CTGGAG 1 cut(s) 581
HaeII RGCGCY 1 cut(s) 1280
HaeIII GGCC 3 cut(s) 150, 363, 764
HapII CCGG 6 cut(s) 236, 360, 421, 436, 445, 1318
HgaI GACGC 2 cut(s) 425, 2423
HhaI GCGC 5 cut(s) 444, 901, 1279, 2025, 2537
Hin1I GRCGYC 1 cut(s) 417
Hin6I GCGC 5 cut(s) 442, 899, 1277, 2023, 2535
HinP1I GCGC 5 cut(s) 442, 899, 1277, 2023, 2535
HindIII AAGCTT 2 cut(s) 1049, 2648
HpaII CCGG 6 cut(s) 236, 360, 421, 436, 445, 1318
HphI GGTGA 4 cut(s) 259, 616, 1105, 1302
Hpy188I TCNGA 2 cut(s) 1800, 2614
Hpy188III TCNNGA 8 cut(s) 530, 845, 1073, 1103, 1283, 1418, 2573, 2657
Hpy99I CGWCG 3 cut(s) 19, 419, 2114
HpyAV CCTTC 9 cut(s) 871, 947, 979, 1660, 1772, 1900, 1925, 2494, 2570
HpyCH4III ACNGT 8 cut(s) 1202, 1436, 1451, 1646, 1738, 1856, 2173, 2626
HpyCH4IV ACGT 5 cut(s) 23, 210, 306, 905, 1752
HpyF10VI GCNNNNNNNGC 1 cut(s) 1160
HpyF3I CTNAG 8 cut(s) 479, 505, 1388, 1715, 1797, 1917, 2613, 2658
HpySE526I ACGT 5 cut(s) 23, 210, 306, 905, 1752
Hsp92I GRCGYC 1 cut(s) 417
HspAI GCGC 5 cut(s) 442, 899, 1277, 2023, 2535
Ksp22I TGATCA 1 cut(s) 1338
Kzo9I GATC 2 cut(s) 660, 1338
LguI GCTCTTC 1 cut(s) 137
LmnI GCTCC 2 cut(s) 1151, 2202
Lsp1109I GCAGC 6 cut(s) 84, 1150, 1890, 2088, 2360, 2411
LweI GCATC 9 cut(s) 886, 1281, 1524, 1573, 1701, 1831, 2023, 2067, 2597
MaeI CTAG 6 cut(s) 999, 1029, 1062, 1073, 1655, 2337
MaeII ACGT 5 cut(s) 23, 210, 306, 905, 1752
MaeIII GTNAC 9 cut(s) 842, 1037, 1111, 1556, 1748, 1829, 2351, 2620, 2642
MalI GATC 2 cut(s) 662, 1340
MbiI CCGCTC 1 cut(s) 1154
MboI GATC 2 cut(s) 660, 1338
MhlI GDGCHC 6 cut(s) 23, 825, 1023, 1266, 2199, 2230
MlyI GAGTC 2 cut(s) 850, 2459
Mph1103I ATGCAT 3 cut(s) 928, 1126, 1873
MroXI GAANNNNTTC 1 cut(s) 1233
MslI CAYNNNNRTG 2 cut(s) 386, 2223
MspA1I CMGCKG 3 cut(s) 75, 110, 2156
MspCI CTTAAG 1 cut(s) 2585
MspI CCGG 6 cut(s) 236, 360, 421, 436, 445, 1318
MspR9I CCNGG 5 cut(s) 236, 271, 421, 1268, 2560
Mva1269I GAATGC 2 cut(s) 613, 1611
MvaI CCWGG 3 cut(s) 271, 1268, 2560
MvnI CGCG 1 cut(s) 2537
MwoI GCNNNNNNNGC 1 cut(s) 1160
NciI CCSGG 2 cut(s) 236, 421
NdeI CATATG 2 cut(s) 2133, 2313
NdeII GATC 2 cut(s) 660, 1338
NheI GCTAGC 1 cut(s) 1061
NlaIV GGNNCC 3 cut(s) 364, 377, 1101
NmeAIII GCCGAG 2 cut(s) 394, 1639
NmuCI GTSAC 3 cut(s) 842, 1037, 1111
NsiI ATGCAT 3 cut(s) 928, 1126, 1873
NspI RCATGY 1 cut(s) 2029
PciSI GCTCTTC 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PctI GAATGC 2 cut(s) 613, 1611
PdmI GAANNNNTTC 1 cut(s) 1233
PflMI CCANNNNNTGG 1 cut(s) 1273
PinAI ACCGGT 1 cut(s) 435
PkrI GCNGC 7 cut(s) 74, 149, 1165, 1880, 2103, 2375, 2401
PleI GAGTC 2 cut(s) 849, 2458
PpsI GAGTC 2 cut(s) 849, 2458
Ppu21I YACGTR 1 cut(s) 906
PshBI ATTAAT 1 cut(s) 1994
PsiI TTATAA 2 cut(s) 1014, 1329
Psp124BI GAGCTC 1 cut(s) 2199
Psp1406I AACGTT 1 cut(s) 1752
Psp6I CCWGG 3 cut(s) 269, 1266, 2558
PspEI GGTNACC 2 cut(s) 1829, 2620
PspGI CCWGG 3 cut(s) 269, 1266, 2558
PspN4I GGNNCC 3 cut(s) 364, 377, 1101
PspPI GGNCC 5 cut(s) 149, 238, 362, 376, 433
PstI CTGCAG 3 cut(s) 1945, 2106, 2522
PstNI CAGNNNCTG 2 cut(s) 75, 1455
PvuII CAGCTG 2 cut(s) 75, 2156
RsaI GTAC 3 cut(s) 863, 1853, 1858
RsaNI GTAC 3 cut(s) 862, 1852, 1857
RseI CAYNNNNRTG 2 cut(s) 386, 2223
SacI GAGCTC 1 cut(s) 2199
SapI GCTCTTC 1 cut(s) 137
SatI GCNGC 7 cut(s) 73, 148, 1164, 1879, 2102, 2374, 2400
Sau3AI GATC 2 cut(s) 660, 1338
Sau96I GGNCC 5 cut(s) 149, 238, 362, 376, 433
SchI GAGTC 2 cut(s) 850, 2459
ScrFI CCNGG 5 cut(s) 236, 271, 421, 1268, 2560
SduI GDGCHC 6 cut(s) 23, 825, 1023, 1266, 2199, 2230
SfaNI GCATC 9 cut(s) 886, 1281, 1524, 1573, 1701, 1831, 2023, 2067, 2597
SfcI CTRYAG 6 cut(s) 67, 76, 1734, 1941, 2102, 2518
SinI GGWCC 3 cut(s) 238, 376, 433
SmiMI CAYNNNNRTG 2 cut(s) 386, 2223
SmlI CTYRAG 4 cut(s) 1482, 1502, 2201, 2585
SmoI CTYRAG 4 cut(s) 1482, 1502, 2201, 2585
SsiI CCGC 8 cut(s) 110, 147, 252, 343, 366, 379, 671, 1154
SspMI CTAG 6 cut(s) 999, 1029, 1062, 1073, 1655, 2337
SstI GAGCTC 1 cut(s) 2199
StyD4I CCNGG 5 cut(s) 234, 269, 419, 1266, 2558
StyI CCWWGG 1 cut(s) 1654
TaaI ACNGT 8 cut(s) 1202, 1436, 1451, 1646, 1738, 1856, 2173, 2626
TaiI ACGT 5 cut(s) 26, 213, 309, 908, 1755
TaqI TCGA 5 cut(s) 1446, 1494, 1578, 1971, 2631
TaqII GACCGA 1 cut(s) 2123
TatI WGTACW 1 cut(s) 861
TauI GCSGC 1 cut(s) 150
TscAI CASTG 5 cut(s) 1355, 1430, 1441, 1704, 2178
TseFI GTSAC 3 cut(s) 842, 1037, 1111
TseI GCWGC 6 cut(s) 72, 1163, 1878, 2101, 2373, 2399
Tsp45I GTSAC 3 cut(s) 842, 1037, 1111
TspGWI ACGGA 1 cut(s) 1315
TspRI CASTG 5 cut(s) 1355, 1430, 1441, 1704, 2178
Van91I CCANNNNNTGG 1 cut(s) 1273
Vha464I CTTAAG 1 cut(s) 2585
VneI GTGCAC 2 cut(s) 1262, 2226
VpaK11BI GGWCC 3 cut(s) 238, 376, 433
VspI ATTAAT 1 cut(s) 1994
XapI RAATTY 6 cut(s) 293, 458, 1814, 1935, 2506, 2523
XbaI TCTAGA 1 cut(s) 1072
XceI RCATGY 1 cut(s) 2029
XmaJI CCTAGG 1 cut(s) 1654
XmnI GAANNNNTTC 1 cut(s) 1233
XspI CTAG 6 cut(s) 999, 1029, 1062, 1073, 1655, 2337
Zsp2I ATGCAT 3 cut(s) 928, 1126, 1873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.