AT4G38250

Transmembrane amino acid transporter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012818
Physical Location & Seq
Forward (+)
17935402 .. 17937068
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G38250.1

Sequence Viewer

Length: 1311 bp
ATGGGTTTTCAGAACGAAGCTAGCTCCTCCTCTTATACCCTTAAAATACCTCCACCGGCCAGAGAGGACTCGCCTCTTCTCGGCAAGGGACCTCCCCTCTCCAGCCAGTTTAAGACCTTCGCCAACGTCTTCATCGCTGTCGTCGGCGCTGGTGTTCTTGGTCTTCCTTACGCCTTCAAGCGAACCGGATGGCTCATGGGTGTCCTCCTCCTCGTCTCCGTCTCTGTTTTGACCCATCACTGTATGATGCTTCTTGTCTATACCCGCCGCAAGCTCGACTCTTTCAATGCTGGCATCTCCAAGATCGGCTCTTTTGGTGACCTTGGATTCGCCGTCTGCGGCTCCTTGGGGAGGATCGTCGTTGACTTATTTATCATTCTGTCTCAAGCTGGTTTTTGTGTCGGATATCTTATCTTCATCGGCACTACTTTAGCTAATCTATCCGATCCTGAATCGCCCACCAGTCTGAGGCATCAGTTTACAAGGCTGGGTTCTGAATTCCTGGGCGTGTCTTCTAAGAGTCTCTACATATGGGGATGCTTTCCCTTTCAGCTCGGTTTGAATTCGATCAAGACGCTCACTCACTTGGCTCCTCTCAGCATCTTCGCCGATATTGTTGATCTTGGCGCTATGGCTGTGGTCATTGTTGAGGATTCCATGATTATACTCAAGCAAAGGCCTGACGTGGTTGCCTTTGGTGGTATGTCCCTCTTCTTGTATGGTATGGGCGTCGCTGTCTATTCCTTCGAAGGCGTTGGAATGGTCTTGCCTCTTGAATCGGAGATGAAGGACAAGGACAAGTTTGGCAAAGTCTTGGCTCTCGGCATGGGATTCATCTCTCTCATATACATCGCATTTGGTATCTTAGGTTACTTGGCTTTTGGTGAGGACACCATGGACATTATCACTGCTAACTTGGGGGCAGGACTTGTCAGCACTGTTGTCCAGCTTGGACTGTGCATCAACCTCTTCTTCACCTTCCCCTTGATGATGAATCCAGTGTTTGAGATAGTGGAGAGGCGTTTCTCACGCGGGATGTACTCTGCGTGGCTCAGATGGGTACTCGTCTTAGCAGTGACTCTGGTGGCTCTTTTTGTGCCAAACTTTGCAGATTTTTTGTCATTGGTTGGCAGCAGCACTTGCTGTGTGTTAGGCTTTGTGTTACCTGCATTGTTTCATTTATTGGTGTTCAAGGAAGAGATGGGGTGGCTGCAATGGAGTTCAGACACAGCGATTGTGGTACTAGGTGTGGTTCTTGCTGTGTCGGGAACTTGGAGTTCCCTGAGTGAGATCTTCTCTGTCAAAGTGTGA

Protein Analysis

436

Amino Acids

47.21

Weight (kDa)

6.22

Isoelectric Point (pI)

31.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 35 - 427 1.6e-73 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012818)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42005 AT4G38250
fragaria_vesca FvH4_2g16760
malus_domestica MD10G1141500.v1.1
prunus_persica Prupe.8G179700_v2.0.a1
pyrus_communis pycom05g13360 pycom10g12110
rosa_chinensis RchiOBHm_Chr6g0280881
rosa_laevigata RLG00000013023
rosa_multiflora Rmu_sc0004772.1_g000003
rosa_roxburghii Rroxscaffold_7G00187530
rosa_rugosa Rorug06G0136000
rosa_samantha Rh6AG248800 Rh6BG250500 Rh6BG250700 Rh6CG251500 Rh6DG243200
rosa_wichuraiana Rw6G021510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1174
AccII CGCG 1 cut(s) 1032
AciI CCGC 4 cut(s) 265, 268, 339, 1032
AclWI GGATC 2 cut(s) 362, 440
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 2 cut(s) 497, 562
AcyI GRCGYC 1 cut(s) 729
AfaI GTAC 3 cut(s) 1040, 1062, 1242
AfiI CCNNNNNNNGG 3 cut(s) 80, 351, 468
AgsI TTSAA 5 cut(s) 178, 286, 562, 776, 1192
AjiI CACGTC 1 cut(s) 685
AjnI CCWGG 1 cut(s) 501
AluBI AGCT 7 cut(s) 20, 24, 274, 389, 434, 553, 949
AluI AGCT 7 cut(s) 20, 24, 274, 389, 434, 553, 949
Alw26I GTCTC 4 cut(s) 220, 226, 387, 527
AlwI GGATC 2 cut(s) 362, 440
AoxI GGCC 2 cut(s) 57, 677
ApeKI GCWGC 3 cut(s) 1131, 1134, 1210
ApoI RAATTY 2 cut(s) 497, 562
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
AspLEI GCGC 2 cut(s) 149, 629
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 3 cut(s) 329, 896, 967
AsuII TTCGAA 1 cut(s) 747
AsuNHI GCTAGC 1 cut(s) 20
AvaII GGWCC 1 cut(s) 89
BbsI GAAGAC 3 cut(s) 121, 155, 504
BbvI GCAGC 3 cut(s) 1143, 1146, 1197
BccI CCATC 4 cut(s) 183, 243, 1050, 1195
BceAI ACGGC 1 cut(s) 317
BciT130I CCWGG 1 cut(s) 503
BcoDI GTCTC 4 cut(s) 220, 226, 387, 527
BfaI CTAG 2 cut(s) 21, 1244
BfoI RGCGCY 2 cut(s) 150, 630
BfuAI ACCTGC 1 cut(s) 1174
BglII AGATCT 1 cut(s) 1290
BisI GCNGC 5 cut(s) 268, 340, 1132, 1135, 1211
BlsI GCNGC 5 cut(s) 269, 341, 1133, 1136, 1212
Bme1390I CCNGG 1 cut(s) 503
Bme18I GGWCC 1 cut(s) 89
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 3 cut(s) 90, 343, 591
BmrFI CCNGG 1 cut(s) 503
BmsI GCATC 6 cut(s) 237, 303, 481, 527, 609, 969
BmtI GCTAGC 1 cut(s) 24
BpiI GAAGAC 3 cut(s) 121, 155, 504
BplI GAGNNNNNCTC 1 cut(s) 1280
BpmI CTGGAG 1 cut(s) 85
Bpu14I TTCGAA 1 cut(s) 747
BpuEI CTTGAG 2 cut(s) 369, 653
BsaHI GRCGYC 1 cut(s) 729
BsaJI CCNNGG 4 cut(s) 322, 345, 502, 894
BsaWI WCCGGW 1 cut(s) 185
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 351, 468
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 106, 462, 998
Bse3DI GCAATG 1 cut(s) 1220
BseBI CCWGG 1 cut(s) 503
BseDI CCNNGG 4 cut(s) 322, 345, 502, 894
BseGI GGATG 3 cut(s) 194, 542, 1041
BseLI CCNNNNNNNGG 3 cut(s) 80, 351, 468
BseMI GCAATG 1 cut(s) 1220
BseMII CTCAG 4 cut(s) 458, 610, 1066, 1274
BseNI ACTGG 3 cut(s) 106, 462, 998
BseRI GAGGAG 5 cut(s) 16, 19, 197, 200, 582
BseXI GCAGC 3 cut(s) 1143, 1146, 1197
BseYI CCCAGC 1 cut(s) 487
Bsh1236I CGCG 1 cut(s) 1032
BshFI GGCC 2 cut(s) 59, 679
BsiSI CCGG 2 cut(s) 56, 186
BslFI GGGAC 2 cut(s) 102, 691
BslI CCNNNNNNNGG 3 cut(s) 80, 351, 468
BsmAI GTCTC 4 cut(s) 220, 226, 387, 527
BsmBI CGTCTC 2 cut(s) 220, 226
BsmFI GGGAC 2 cut(s) 102, 691
BsnI GGCC 2 cut(s) 59, 679
Bsp119I TTCGAA 1 cut(s) 747
Bsp143I GATC 6 cut(s) 303, 354, 445, 567, 619, 1290
Bsp19I CCATGG 1 cut(s) 894
BspACI CCGC 4 cut(s) 265, 268, 339, 1032
BspANI GGCC 2 cut(s) 59, 679
BspCNI CTCAG 4 cut(s) 459, 609, 1065, 1275
BspFNI CGCG 1 cut(s) 1032
BspLI GGNNCC 3 cut(s) 90, 343, 591
BspMI ACCTGC 1 cut(s) 1174
BspOI GCTAGC 1 cut(s) 24
BspPI GGATC 2 cut(s) 362, 440
BspT104I TTCGAA 1 cut(s) 747
BsrDI GCAATG 1 cut(s) 1220
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 3 cut(s) 106, 462, 998
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 4 cut(s) 322, 345, 502, 894
BssMI GATC 6 cut(s) 303, 354, 445, 567, 619, 1290
BssNI GRCGYC 1 cut(s) 729
BssT1I CCWWGG 3 cut(s) 322, 345, 894
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 3 cut(s) 242, 940, 957
Bst6I CTCTTC 4 cut(s) 81, 716, 974, 1191
BstACI GRCGYC 1 cut(s) 729
BstAPI GCANNNNNTGC 1 cut(s) 1140
BstBI TTCGAA 1 cut(s) 747
BstC8I GCNNGC 3 cut(s) 22, 272, 292
BstDEI CTNAG 7 cut(s) 467, 516, 596, 865, 1052, 1069, 1283
BstDSI CCRYGG 1 cut(s) 894
BstEII GGTNACC 1 cut(s) 317
BstENI CCTNNNNNAGG 1 cut(s) 349
BstF5I GGATG 3 cut(s) 194, 542, 1041
BstFNI CGCG 1 cut(s) 1032
BstH2I RGCGCY 2 cut(s) 150, 630
BstHHI GCGC 2 cut(s) 149, 629
BstKTI GATC 6 cut(s) 306, 357, 448, 570, 622, 1293
BstMAI GTCTC 4 cut(s) 220, 226, 387, 527
BstMBI GATC 6 cut(s) 303, 354, 445, 567, 619, 1290
BstMWI GCNNNNNNNGC 1 cut(s) 1140
BstNI CCWGG 1 cut(s) 503
BstPI GGTNACC 1 cut(s) 317
BstSCI CCNGG 1 cut(s) 501
BstUI CGCG 1 cut(s) 1032
BstV1I GCAGC 3 cut(s) 1143, 1146, 1197
BstV2I GAAGAC 3 cut(s) 121, 155, 504
BstX2I RGATCY 1 cut(s) 1290
BstYI RGATCY 1 cut(s) 1290
BsuRI GGCC 2 cut(s) 59, 679
BtgI CCRYGG 1 cut(s) 894
BtgZI GCGATG 2 cut(s) 118, 835
BtrI CACGTC 1 cut(s) 685
BtsCI GGATG 3 cut(s) 194, 542, 1041
BtsI GCAGTG 2 cut(s) 906, 1080
BtsIMutI CAGTG 5 cut(s) 238, 906, 936, 1005, 1080
BveI ACCTGC 1 cut(s) 1174
Cac8I GCNNGC 3 cut(s) 22, 272, 292
CfoI GCGC 2 cut(s) 149, 629
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 89
CseI GACGC 2 cut(s) 583, 718
Csp6I GTAC 3 cut(s) 1039, 1061, 1241
CviAII CATG 4 cut(s) 196, 658, 826, 895
CviQI GTAC 3 cut(s) 1039, 1061, 1241
DdeI CTNAG 7 cut(s) 467, 516, 596, 865, 1052, 1069, 1283
DpnI GATC 6 cut(s) 305, 356, 447, 569, 621, 1292
DpnII GATC 6 cut(s) 303, 354, 445, 567, 619, 1290
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 4 cut(s) 81, 716, 974, 1191
EarI CTCTTC 4 cut(s) 81, 716, 974, 1191
Eco130I CCWWGG 3 cut(s) 322, 345, 894
Eco147I AGGCCT 1 cut(s) 679
Eco32I GATATC 1 cut(s) 407
Eco47I GGWCC 1 cut(s) 89
Eco91I GGTNACC 1 cut(s) 317
EcoNI CCTNNNNNAGG 1 cut(s) 349
EcoO109I RGGNCCY 1 cut(s) 89
EcoO65I GGTNACC 1 cut(s) 317
EcoRI GAATTC 2 cut(s) 497, 562
EcoRII CCWGG 1 cut(s) 501
EcoRV GATATC 1 cut(s) 407
EcoT14I CCWWGG 3 cut(s) 322, 345, 894
ErhI CCWWGG 3 cut(s) 322, 345, 894
Esp3I CGTCTC 2 cut(s) 220, 226
FaeI CATG 4 cut(s) 199, 661, 829, 898
FaqI GGGAC 2 cut(s) 102, 691
FatI CATG 4 cut(s) 195, 657, 825, 894
FauI CCCGC 2 cut(s) 272, 1025
FauNDI CATATG 1 cut(s) 530
Fnu4HI GCNGC 5 cut(s) 268, 340, 1132, 1135, 1211
FokI GGATG 3 cut(s) 201, 549, 1048
Fsp4HI GCNGC 5 cut(s) 268, 340, 1132, 1135, 1211
FspBI CTAG 2 cut(s) 21, 1244
GlaI GCGC 2 cut(s) 148, 628
GluI GCNGC 5 cut(s) 268, 340, 1132, 1135, 1211
GsaI CCCAGC 1 cut(s) 491
GsuI CTGGAG 1 cut(s) 85
HaeII RGCGCY 2 cut(s) 150, 630
HaeIII GGCC 2 cut(s) 59, 679
HapII CCGG 2 cut(s) 56, 186
HgaI GACGC 2 cut(s) 583, 718
HhaI GCGC 2 cut(s) 149, 629
Hin1I GRCGYC 1 cut(s) 729
Hin1II CATG 4 cut(s) 199, 661, 829, 898
Hin6I GCGC 2 cut(s) 147, 627
HinP1I GCGC 2 cut(s) 147, 627
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HpaII CCGG 2 cut(s) 56, 186
HphI GGTGA 3 cut(s) 329, 896, 967
Hpy166II GTNNAC 2 cut(s) 364, 480
Hpy188I TCNGA 8 cut(s) 12, 404, 445, 468, 496, 781, 1055, 1225
Hpy188III TCNNGA 4 cut(s) 449, 571, 773, 1266
Hpy8I GTNNAC 2 cut(s) 364, 480
Hpy99I CGWCG 3 cut(s) 146, 362, 734
HpyAV CCTTC 6 cut(s) 127, 184, 743, 754, 781, 988
HpyCH4III ACNGT 3 cut(s) 242, 940, 957
HpyCH4IV ACGT 2 cut(s) 126, 684
HpyCH4V TGCA 4 cut(s) 960, 1109, 1169, 1213
HpyF10VI GCNNNNNNNGC 1 cut(s) 1140
HpyF3I CTNAG 7 cut(s) 467, 516, 596, 865, 1052, 1069, 1283
HpySE526I ACGT 2 cut(s) 126, 684
Hsp92I GRCGYC 1 cut(s) 729
Hsp92II CATG 4 cut(s) 199, 661, 829, 898
HspAI GCGC 2 cut(s) 147, 627
Kzo9I GATC 6 cut(s) 303, 354, 445, 567, 619, 1290
LmnI GCTCC 3 cut(s) 29, 347, 595
Lsp1109I GCAGC 3 cut(s) 1143, 1146, 1197
LweI GCATC 6 cut(s) 237, 303, 481, 527, 609, 969
MaeI CTAG 2 cut(s) 21, 1244
MaeII ACGT 2 cut(s) 126, 684
MaeIII GTNAC 4 cut(s) 317, 869, 1075, 1161
MalI GATC 6 cut(s) 305, 356, 447, 569, 621, 1292
MboI GATC 6 cut(s) 303, 354, 445, 567, 619, 1290
MflI RGATCY 1 cut(s) 1290
MluCI AATT 2 cut(s) 497, 562
MlyI GAGTC 4 cut(s) 62, 272, 529, 1072
MmeI TCCRAC 2 cut(s) 382, 736
MseI TTAA 2 cut(s) 42, 111
MspI CCGG 2 cut(s) 56, 186
MspR9I CCNGG 1 cut(s) 503
MvaI CCWGG 1 cut(s) 503
MvnI CGCG 1 cut(s) 1032
MwoI GCNNNNNNNGC 1 cut(s) 1140
NcoI CCATGG 1 cut(s) 894
NdeI CATATG 1 cut(s) 530
NdeII GATC 6 cut(s) 303, 354, 445, 567, 619, 1290
NheI GCTAGC 1 cut(s) 20
NlaIII CATG 4 cut(s) 199, 661, 829, 898
NlaIV GGNNCC 3 cut(s) 90, 343, 591
NmeAIII GCCGAG 2 cut(s) 60, 801
NmuCI GTSAC 2 cut(s) 317, 1075
NspV TTCGAA 1 cut(s) 747
PceI AGGCCT 1 cut(s) 679
PfeI GAWTC 6 cut(s) 327, 452, 653, 776, 831, 994
PkrI GCNGC 5 cut(s) 269, 341, 1133, 1136, 1212
PleI GAGTC 4 cut(s) 62, 272, 528, 1072
PpsI GAGTC 4 cut(s) 62, 272, 528, 1072
PpuMI RGGWCCY 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 501
PspEI GGTNACC 1 cut(s) 317
PspFI CCCAGC 1 cut(s) 487
PspGI CCWGG 1 cut(s) 501
PspN4I GGNNCC 3 cut(s) 90, 343, 591
PspPI GGNCC 1 cut(s) 89
PspPPI RGGWCCY 1 cut(s) 89
PsuI RGATCY 1 cut(s) 1290
RsaI GTAC 3 cut(s) 1040, 1062, 1242
RsaNI GTAC 3 cut(s) 1039, 1061, 1241
SaqAI TTAA 2 cut(s) 42, 111
SatI GCNGC 5 cut(s) 268, 340, 1132, 1135, 1211
Sau3AI GATC 6 cut(s) 303, 354, 445, 567, 619, 1290
Sau96I GGNCC 1 cut(s) 89
SchI GAGTC 4 cut(s) 62, 272, 529, 1072
ScrFI CCNGG 1 cut(s) 503
SfaNI GCATC 6 cut(s) 237, 303, 481, 527, 609, 969
SfuI TTCGAA 1 cut(s) 747
SinI GGWCC 1 cut(s) 89
SmlI CTYRAG 2 cut(s) 384, 668
SmoI CTYRAG 2 cut(s) 384, 668
Sse9I AATT 2 cut(s) 497, 562
SseBI AGGCCT 1 cut(s) 679
SsiI CCGC 4 cut(s) 265, 268, 339, 1032
SspMI CTAG 2 cut(s) 21, 1244
StuI AGGCCT 1 cut(s) 679
StyD4I CCNGG 1 cut(s) 501
StyI CCWWGG 3 cut(s) 322, 345, 894
TaaI ACNGT 3 cut(s) 242, 940, 957
TaiI ACGT 2 cut(s) 129, 687
TaqI TCGA 3 cut(s) 276, 566, 747
TasI AATT 2 cut(s) 497, 562
TatI WGTACW 1 cut(s) 1038
TauI GCSGC 2 cut(s) 270, 342
TfiI GAWTC 6 cut(s) 327, 452, 653, 776, 831, 994
Tru1I TTAA 2 cut(s) 42, 111
Tru9I TTAA 2 cut(s) 42, 111
TscAI CASTG 5 cut(s) 245, 913, 943, 1005, 1080
TseFI GTSAC 2 cut(s) 317, 1075
TseI GCWGC 3 cut(s) 1131, 1134, 1210
Tsp45I GTSAC 2 cut(s) 317, 1075
TspDTI ATGAA 6 cut(s) 121, 406, 800, 823, 1007, 1166
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 5 cut(s) 245, 913, 943, 1005, 1080
VpaK11BI GGWCC 1 cut(s) 89
XagI CCTNNNNNAGG 1 cut(s) 349
XapI RAATTY 2 cut(s) 497, 562
XspI CTAG 2 cut(s) 21, 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.