pycom05g13360

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012818
Physical Location & Seq
Reverse (-)
16948031 .. 16949294
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13360.2

Sequence Viewer

Length: 1191 bp
ATGGGTGGCGACAAGGAGGCAACCTCGTCGTCCCAGCGTCTCAGGTTGCCGCCCCCGACGGAGAACACGCCGCTGATCGGCGACACCAAGCCATTGTCTTCCCAATCCAAAACCTTTGCCAACATCTTCATCGCCGTTGTAGGCGCCGGTGTTCTCGGTCTGCCGTATTCCTTCAAGCGCACCGGGTGGCTCATGAGCCTCCTCACGCTCCTCCTCGTCGCGTTCCTCACCCACCATTGCATGATGCTGCTGGTCTACACCAGAAGAAAGCTCGAGTCTTCCTATGGGTTCACAAAGATCCCGTCTTTTGGGGATTGGGTTTCGCCGTTTGCGGCATTTGGGGGCGGTTGCTGCACATTTGCTCAATGCACCCACAGCATCCGATTTGACACCCAAGATATTGGGGTTGGCACCCAAAAGCTACTACATTTGGGGGTGTTTCCCCTTCCAATTGGGGTTGAATTCGATTGCAACGCTGACCCATTTGGCCCCTCTGAGTATTTTTGCTCAATGGGGGTTGTGATGGTTAAGGATGTGGCGGTTATGTTGCAAAAAATGCCAGAAGTGAAGGCATTTGGAGACCTTTCTCTGTTCTTTTACGGGCTGGGCGTGGCGGTCTACTCCTTCGAAGGGTTTGGGATGGTTCTGCCATTGGAGTCTGAGGCGAAAGACAAGGACAAGTTTGGGAAGATTCTTGCTTGGACAATGACATTCATTTCTCTGATGTATGGAAACTTTGGAGCTCTTGGTTACTTTGCATTTGGGGAACAAACCAAGGATATGATCGAGGCTAATTATGGTGTGGGATTGATTAGTACCCTGGTGAAGCTTGGCCTTGTGGTGAATCTGTTCTTCACATTGCCTATAATGATGAACCCGGTTTATGAGATTGTTGAAAGGCGGTTTTTGGGTGGGAGGTCTTGCTTGTGGCTGAGATGGGTGCTGGTTTTGGCAGTGAGCTTGGTAGCATTGTCGGTCCCCAATTTCACGGATTTCATGTCGTTGGTGGGGAGTGGTGTGTGTTGTGCATTAGGGTTTGTTGTGCCGACTCTGTTTCATCTGATCGTGTTCAAGGGAGAGATGGGGTGGGGGCAGTGGTGCTCAGATATGAGTATTTTGGTGTTGGGGGTTGTTCTTGCGGTTTCAGGAACTTGGTCTGCTCTGCTAGAGATCTTTGCTGCCAAAGTATAG

Protein Analysis

397

Amino Acids

43.29

Weight (kDa)

6.52

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012818)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42005 AT4G38250
fragaria_vesca FvH4_2g16760
malus_domestica MD10G1141500.v1.1
prunus_persica Prupe.8G179700_v2.0.a1
pyrus_communis pycom05g13360 pycom10g12110
rosa_chinensis RchiOBHm_Chr6g0280881
rosa_laevigata RLG00000013023
rosa_multiflora Rmu_sc0004772.1_g000003
rosa_roxburghii Rroxscaffold_7G00187530
rosa_rugosa Rorug06G0136000
rosa_samantha Rh6AG248800 Rh6BG250500 Rh6BG250700 Rh6CG251500 Rh6DG243200
rosa_wichuraiana Rw6G021510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 143, 410
AccI GTMKAC 2 cut(s) 255, 618
AccII CGCG 1 cut(s) 221
AciI CCGC 8 cut(s) 50, 71, 332, 345, 539, 614, 901, 1139
AclWI GGATC 1 cut(s) 292
AcsI RAATTY 1 cut(s) 461
AcyI GRCGYC 1 cut(s) 144
AdeI CACNNNGTG 1 cut(s) 186
AfaI GTAC 1 cut(s) 817
AfiI CCNNNNNNNGG 3 cut(s) 77, 308, 630
AgsI TTSAA 4 cut(s) 175, 461, 896, 1072
AjnI CCWGG 1 cut(s) 819
AluBI AGCT 5 cut(s) 271, 421, 743, 829, 960
AluI AGCT 5 cut(s) 271, 421, 743, 829, 960
Alw21I GWGCWC 2 cut(s) 745, 1103
Alw26I GTCTC 2 cut(s) 44, 573
AlwI GGATC 1 cut(s) 292
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 2 cut(s) 487, 832
ApeKI GCWGC 3 cut(s) 247, 351, 1178
ApoI RAATTY 1 cut(s) 461
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
Asp700I GAANNNNTTC 1 cut(s) 848
AspLEI GCGC 2 cut(s) 146, 180
AspS9I GGNCC 2 cut(s) 488, 976
AsuC2I CCSGG 2 cut(s) 184, 878
AsuHPI GGTGA 3 cut(s) 220, 835, 853
AsuII TTCGAA 1 cut(s) 627
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 1 cut(s) 976
BanI GGYRCC 2 cut(s) 143, 410
BanII GRGCYC 1 cut(s) 745
BbsI GAAGAC 2 cut(s) 90, 270
Bbv12I GWGCWC 2 cut(s) 745, 1103
BbvI GCAGC 3 cut(s) 234, 338, 1165
BccI CCATC 4 cut(s) 517, 634, 930, 1075
BceAI ACGGC 3 cut(s) 119, 148, 310
BcgI CGANNNNNNTGC 2 cut(s) 9, 43
BciT130I CCWGG 1 cut(s) 821
BcnI CCSGG 2 cut(s) 184, 878
BcoDI GTCTC 2 cut(s) 44, 573
BfaI CTAG 1 cut(s) 1166
BfoI RGCGCY 1 cut(s) 147
BglII AGATCT 1 cut(s) 1170
BisI GCNGC 6 cut(s) 50, 71, 248, 333, 352, 1179
BlsI GCNGC 6 cut(s) 51, 72, 249, 334, 353, 1180
Bme1390I CCNGG 3 cut(s) 184, 821, 878
Bme18I GGWCC 1 cut(s) 976
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 2 cut(s) 488, 976
BmiI GGNNCC 4 cut(s) 145, 412, 490, 978
BmrFI CCNGG 3 cut(s) 184, 821, 878
BmsI GCATC 2 cut(s) 234, 387
BpiI GAAGAC 2 cut(s) 90, 270
BplI GAGNNNNNCTC 2 cut(s) 8, 40
Bpu14I TTCGAA 1 cut(s) 627
BpuMI CCSGG 2 cut(s) 184, 878
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 573
BsaJI CCNNGG 2 cut(s) 774, 819
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 308, 630
Bse118I RCCGGY 1 cut(s) 146
Bse3DI GCAATG 2 cut(s) 235, 857
BseBI CCWGG 1 cut(s) 821
BseDI CCNNGG 2 cut(s) 774, 819
BseGI GGATG 3 cut(s) 378, 538, 645
BseLI CCNNNNNNNGG 3 cut(s) 77, 308, 630
BseMI GCAATG 2 cut(s) 235, 857
BseMII CTCAG 5 cut(s) 55, 486, 651, 923, 1116
BseRI GAGGAG 3 cut(s) 191, 200, 203
BseXI GCAGC 3 cut(s) 234, 338, 1165
BseYI CCCAGC 2 cut(s) 33, 604
BsgI GTGCAG 1 cut(s) 337
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 2 cut(s) 489, 834
BshNI GGYRCC 2 cut(s) 143, 410
BsiHKAI GWGCWC 2 cut(s) 745, 1103
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 3 cut(s) 147, 183, 878
BslFI GGGAC 2 cut(s) 16, 962
BslI CCNNNNNNNGG 3 cut(s) 77, 308, 630
BsmAI GTCTC 2 cut(s) 44, 573
BsmBI CGTCTC 1 cut(s) 44
BsmFI GGGAC 2 cut(s) 16, 962
BsnI GGCC 2 cut(s) 489, 834
Bso31I GGTCTC 1 cut(s) 573
BsoBI CYCGRG 1 cut(s) 272
Bsp119I TTCGAA 1 cut(s) 627
Bsp1286I GDGCHC 2 cut(s) 745, 1103
Bsp143I GATC 5 cut(s) 75, 297, 783, 1062, 1170
BspACI CCGC 8 cut(s) 50, 71, 332, 345, 539, 614, 901, 1139
BspANI GGCC 2 cut(s) 489, 834
BspCNI CTCAG 5 cut(s) 54, 487, 652, 924, 1115
BspFNI CGCG 1 cut(s) 221
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 4 cut(s) 145, 412, 490, 978
BspPI GGATC 1 cut(s) 292
BspT104I TTCGAA 1 cut(s) 627
BspT107I GGYRCC 2 cut(s) 143, 410
BspTNI GGTCTC 1 cut(s) 573
BsrDI GCAATG 2 cut(s) 235, 857
BsrFI RCCGGY 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 2 cut(s) 774, 819
BssMI GATC 5 cut(s) 75, 297, 783, 1062, 1170
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 774
Bst2UI CCWGG 1 cut(s) 821
BstACI GRCGYC 1 cut(s) 144
BstAPI GCANNNNNTGC 1 cut(s) 556
BstBI TTCGAA 1 cut(s) 627
BstDEI CTNAG 5 cut(s) 41, 495, 660, 932, 1102
BstF5I GGATG 3 cut(s) 378, 538, 645
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 2 cut(s) 146, 180
BstKTI GATC 5 cut(s) 78, 300, 786, 1065, 1173
BstMAI GTCTC 2 cut(s) 44, 573
BstMBI GATC 5 cut(s) 75, 297, 783, 1062, 1170
BstMWI GCNNNNNNNGC 3 cut(s) 351, 375, 556
BstNI CCWGG 1 cut(s) 821
BstSCI CCNGG 3 cut(s) 182, 819, 876
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 3 cut(s) 234, 338, 1165
BstV2I GAAGAC 2 cut(s) 90, 270
BstX2I RGATCY 2 cut(s) 297, 1170
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 2 cut(s) 297, 1170
BsuRI GGCC 2 cut(s) 489, 834
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 3 cut(s) 378, 538, 645
BtsI GCAGTG 2 cut(s) 960, 1100
BtsIMutI CAGTG 2 cut(s) 960, 1100
CciI TCATGA 1 cut(s) 192
CfoI GCGC 2 cut(s) 146, 180
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 488, 976
CseI GACGC 1 cut(s) 26
Csp6I GTAC 1 cut(s) 816
CviAII CATG 3 cut(s) 193, 241, 997
CviQI GTAC 1 cut(s) 816
DdeI CTNAG 5 cut(s) 41, 495, 660, 932, 1102
DinI GGCGCC 1 cut(s) 145
DpnI GATC 5 cut(s) 77, 299, 785, 1064, 1172
DpnII GATC 5 cut(s) 75, 297, 783, 1062, 1170
DraIII CACNNNGTG 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 743
Eco130I CCWWGG 1 cut(s) 774
Eco24I GRGCYC 1 cut(s) 745
Eco31I GGTCTC 1 cut(s) 573
Eco47I GGWCC 1 cut(s) 976
Eco53kI GAGCTC 1 cut(s) 743
Eco88I CYCGRG 1 cut(s) 272
EcoICRI GAGCTC 1 cut(s) 743
EcoRI GAATTC 1 cut(s) 461
EcoRII CCWGG 1 cut(s) 819
EcoT14I CCWWGG 1 cut(s) 774
EcoT38I GRGCYC 1 cut(s) 745
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 774
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 3 cut(s) 196, 244, 1000
FaqI GGGAC 2 cut(s) 16, 962
FatI CATG 3 cut(s) 192, 240, 996
FblI GTMKAC 2 cut(s) 255, 618
Fnu4HI GCNGC 6 cut(s) 50, 71, 248, 333, 352, 1179
FokI GGATG 3 cut(s) 365, 545, 652
FriOI GRGCYC 1 cut(s) 745
Fsp4HI GCNGC 6 cut(s) 50, 71, 248, 333, 352, 1179
FspBI CTAG 1 cut(s) 1166
GlaI GCGC 2 cut(s) 145, 179
GluI GCNGC 6 cut(s) 50, 71, 248, 333, 352, 1179
GsaI CCCAGC 2 cut(s) 37, 608
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 2 cut(s) 489, 834
HapII CCGG 3 cut(s) 147, 183, 878
HgaI GACGC 1 cut(s) 26
HhaI GCGC 2 cut(s) 146, 180
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 3 cut(s) 196, 244, 1000
Hin6I GCGC 2 cut(s) 144, 178
HinP1I GCGC 2 cut(s) 144, 178
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 5 cut(s) 275, 656, 691, 844, 1048
HpaII CCGG 3 cut(s) 147, 183, 878
HphI GGTGA 3 cut(s) 220, 835, 853
Hpy166II GTNNAC 3 cut(s) 256, 291, 619
Hpy188I TCNGA 6 cut(s) 383, 496, 661, 723, 1062, 1105
Hpy188III TCNNGA 2 cut(s) 193, 1146
Hpy8I GTNNAC 3 cut(s) 256, 291, 619
Hpy99I CGWCG 3 cut(s) 31, 61, 221
HpyAV CCTTC 5 cut(s) 181, 455, 562, 623, 634
HpyCH4V TGCA 7 cut(s) 240, 354, 369, 471, 550, 758, 1028
HpyF10VI GCNNNNNNNGC 3 cut(s) 351, 375, 556
HpyF3I CTNAG 5 cut(s) 41, 495, 660, 932, 1102
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 3 cut(s) 196, 244, 1000
HspAI GCGC 2 cut(s) 144, 178
KasI GGCGCC 1 cut(s) 143
Kzo9I GATC 5 cut(s) 75, 297, 783, 1062, 1170
LmnI GCTCC 2 cut(s) 213, 740
Lsp1109I GCAGC 3 cut(s) 234, 338, 1165
LweI GCATC 2 cut(s) 234, 387
MaeI CTAG 1 cut(s) 1166
MaeIII GTNAC 1 cut(s) 749
MalI GATC 5 cut(s) 77, 299, 785, 1064, 1172
MboI GATC 5 cut(s) 75, 297, 783, 1062, 1170
MboII GAAGA 6 cut(s) 90, 118, 270, 276, 700, 844
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 2 cut(s) 297, 1170
MhlI GDGCHC 2 cut(s) 745, 1103
MluCI AATT 4 cut(s) 450, 461, 793, 982
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 3 cut(s) 284, 665, 1042
MroXI GAANNNNTTC 1 cut(s) 848
MseI TTAA 1 cut(s) 528
MspA1I CMGCKG 1 cut(s) 73
MspI CCGG 3 cut(s) 147, 183, 878
MspR9I CCNGG 3 cut(s) 184, 821, 878
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 821
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 3 cut(s) 351, 375, 556
NarI GGCGCC 1 cut(s) 144
NciI CCSGG 2 cut(s) 184, 878
NdeII GATC 5 cut(s) 75, 297, 783, 1062, 1170
NlaIII CATG 3 cut(s) 196, 244, 1000
NlaIV GGNNCC 4 cut(s) 145, 412, 490, 978
NspV TTCGAA 1 cut(s) 627
PaeR7I CTCGAG 1 cut(s) 272
PagI TCATGA 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PdmI GAANNNNTTC 1 cut(s) 848
PfeI GAWTC 2 cut(s) 691, 844
PkrI GCNGC 6 cut(s) 51, 72, 249, 334, 353, 1180
PleI GAGTC 3 cut(s) 283, 664, 1042
PluTI GGCGCC 1 cut(s) 147
PpsI GAGTC 3 cut(s) 283, 664, 1042
Psp124BI GAGCTC 1 cut(s) 745
Psp6I CCWGG 1 cut(s) 819
PspFI CCCAGC 2 cut(s) 33, 604
PspGI CCWGG 1 cut(s) 819
PspN4I GGNNCC 4 cut(s) 145, 412, 490, 978
PspPI GGNCC 2 cut(s) 488, 976
PspXI VCTCGAGB 1 cut(s) 272
PsuI RGATCY 2 cut(s) 297, 1170
RsaI GTAC 1 cut(s) 817
RsaNI GTAC 1 cut(s) 816
SacI GAGCTC 1 cut(s) 745
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 6 cut(s) 50, 71, 248, 333, 352, 1179
Sau3AI GATC 5 cut(s) 75, 297, 783, 1062, 1170
Sau96I GGNCC 2 cut(s) 488, 976
SchI GAGTC 3 cut(s) 284, 665, 1042
ScrFI CCNGG 3 cut(s) 184, 821, 878
SduI GDGCHC 2 cut(s) 745, 1103
SfaNI GCATC 2 cut(s) 234, 387
SfoI GGCGCC 1 cut(s) 145
Sfr274I CTCGAG 1 cut(s) 272
SfuI TTCGAA 1 cut(s) 627
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 1 cut(s) 976
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 4 cut(s) 450, 461, 793, 982
SsiI CCGC 8 cut(s) 50, 71, 332, 345, 539, 614, 901, 1139
SspDI GGCGCC 1 cut(s) 143
SspMI CTAG 1 cut(s) 1166
SstI GAGCTC 1 cut(s) 745
StyD4I CCNGG 3 cut(s) 182, 819, 876
StyI CCWWGG 1 cut(s) 774
TaqI TCGA 4 cut(s) 273, 465, 627, 786
TaqII GACCGA 2 cut(s) 146, 964
TasI AATT 4 cut(s) 450, 461, 793, 982
TauI GCSGC 3 cut(s) 52, 73, 335
TfiI GAWTC 2 cut(s) 691, 844
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 2 cut(s) 960, 1100
TseI GCWGC 3 cut(s) 247, 351, 1178
TspDTI ATGAA 5 cut(s) 118, 703, 887, 985, 1046
TspGWI ACGGA 2 cut(s) 74, 1004
TspRI CASTG 2 cut(s) 960, 1100
VpaK11BI GGWCC 1 cut(s) 976
XapI RAATTY 1 cut(s) 461
XhoI CTCGAG 1 cut(s) 272
XmiI GTMKAC 2 cut(s) 255, 618
XmnI GAANNNNTTC 1 cut(s) 848
XspI CTAG 1 cut(s) 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.