Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002900

Physical Location & Seq

Genomic Coordinates Reverse (-)

17958096 .. 17966962

Gene Structure 8867 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G38350.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3822 bp

ATGGCGATTCCTACCAAGCTTCTCCTCTTACAGCTCTTTACTTTCTCACTTTTACTCACCGCAAACCTGACCCACTCAGAACCAAGTAATAATAGACATTCAAAAGAGTATTGCGCTATGTATGACATTTGCGGACACCGCAGCGATGGAAAAGTCCTTAACTGTCCTTATGCCTCCCCTTCCATTCAGCCTGATGAACTGTTTTCTGCCAAAATCCAAAGCCTTTGCCCAACCATAAGTGGCAATGTCTGTTGTACAGAGACTCAGTTTGACACTTTGAGGTCTCAAGTTCAACAGGCTGTCCCTTTTCTTGTGGGTTGTCCTGCATGCTTACGGAACTTCTTAAATTTGTTCTGCGAGCTCTCCTGTTCTCCAAATCAGTCTCTTTTCATCAACGTCACTTCTGTTGCTGAGGTGAGTGGAAATTTGACAGTGGATGGTATAGACTATCACATAACTGATACGTTTGGAGAAGGCCTTTACGAGTCCTGTAAAGAGGTGAAATTTGGAACAATGAACACACGTGCAATTAACTTTGTTGGCGGTGGTGCTAAAAACTTCAGAGAATGGTTTACGTTTATTGGTCAGAAAGCACCATCTGGCTTTCCTGGTTCACCATATGCGATCAACTTCAAATCAAGCATCCCTGAGTCATCTGCGATGGTACCAATGAATGTATCTGTTTATTCATGTGGTGACACATCCCTTGGATGCTCTTGTGGTGATTGCCCTTCCTCACCAGCTTGCTCAAGTCCCGAACCCCTGCCGCCCCATGATGAAGATTCTTGCTCAATTAGAATCGGTCCTCTTAAGGTTAGATGCATTGAGTTATCAATGGCACTTGTATACGTCTTGCTGGTGTCTTGCTTCTTCGGATGGGCTGGTTTAAATCGAAGAAGAAATACGACACAGCCTCTTGATAGCTCAAAGCCACTATTGCATCCTGTGGAAGAAGATGGAATTAATAGTGAAATGAAGGAAAACATACTTGGTGTCAAGGTGCAAAGACATGCTCAACTCTCTCCTGTTCAGAGATATATGGCAAAATTTTACAGATCATATGGGTCATGGATTGCTAGAAATCCATCTCTTGTTCTCTTCATGTCAGTAGCTATTGTTCTTGCACTCTGTTCAGGTCTCTATAATTTCAAGGTGGAAACACGACCAGAAAAGTTGTGGGTTGGTCCTGAAAGCAAAGCAGCAGAAGAGAAAAAGTTCTTTGATACCCACCTTTCGCCATTCTACAGAATCGAGCAGCTTATCTTGGCCACTGTTCCTGATCCCAAAAGTGGCAGGGCGCCTAGTATTGTAACAGATGAAAATATTCTTTTGCTTTTTGACATACAACAGAAGGTTGATCAAATTCGTGGAAATTATTCAGGTTCAGAGGTCTCTTTGCCCGACATATGTTTGAAGCCTTTGGGAGAGGATTGCGCCACTCAGAGTATTCTGCAGTATTTCAAGATGGACTCTGGAACTTTTGATGATTATGGAGGAGTTGAGCATGCTGAGTACTGTTTCCAGCATTACACTTCATCAGAGACGTGTTTGAGCGCTTTTCAGGCGCCTGTTGATCCTAGTGCAGTGTTGGGAGGATTCTCGGGGAATAATTACTCAGAGGCTACTGCGTTTGTTGTAACATATCCAGTCAACAACGTCATTGGTGATTCAAGCAACGAAAACGCAAGGGCAGTAGCATGGGAAAAATCGTTCATTCAATTAGCAAAGGAGGAGCTGCTACCTATGGTGCGATCCAAGAATTTGAGTCTGTCATTTTCATCCGAAAGCTCAATAGAGGAAGAGCTGAAAAGAGAAAGTACTGCTGATGTTATAACAATAGCTGCAAGCTATTTGGTTATGTTTGTTTACATTTCGGTCACACTTGGAGATGCTCCGCAGTTCTATACCTTTTACATTTCATCAAAGGTTTTGCTCGGTCTTTCGGGGGTTGTGCTTGTCTTGCTTTCTGTACTTGGATCTGTTGGAGTTTTCAGTGCCCTTGGAGTTAAATCCACATTGATCATAATGGAAGTCATTCCTTTCCTAGTCCTGGCTGTGGGAGTAGATAATATGTGCATACTGGTTCATGCTGTAAAGAGACAACCTCGTGAGGTTTCTTTAGAGCAACGAATTAGCTCTGCACTTGTTGAAGTAGGTCCATCCATAACATTGGCAAGTTTATCCGAGGTCTTGGCTTTTGCTGTTGGAGCTTTTGTTCCAATGCCAGCATGTCGTATCTTCTCCATGTTTGCAGCATTGGCTATTATGCTGGACTTCTTTCTGCAAATCACCGCTTTTGTTGCGCTAATAGTTTTTGACTGCAAAAGATCTGCGGATAATAGGATTGATTGTTTCCCTTGCATAAAAGTTCCTTCATCATCTCGAGAATCTGTTGAAGGTGGAAGGGAACCTGGGTTTCTTGAGAGATACATGAAGGAGGTTCATGCACCCGTTCTTGGACTTTGGGGTGTAAAAATGGTTGTTGTCGCTGTATTCTTCGCTTTTGCACTGGCAAGCATTGCCATCAGCCCAAGGCTTGAAACTGGCTTGGAGCAGAAAATTGTTCTTCCTCGAGACTCCTACCTTCAAGACTATTTTGACAGTCTCTCAGAGTACCTCAGAGTGGGGCCGCCTTTGTATTTTGTTGTCAAGAATTACAATTATAGCTCGGAATCAAGGCATACAAATCAGTTGTGCTCTATCAGCCAATGCAATTCGAATTCCCTTTTAAATGAGATATCAAGAGCGTCACAGGCTTCAGATACAAGTTACATTGCCAAACCAGCTGCTTCTTGGCTCGATGACTTTCTTGTATGGCTATCACCAGAGGCTTTTGGCTGTTGTCGGAAGTTCACTAATGGCAGTTATTGTCCTCCAGATGACCAGCCACCTTGCTGCACGGCTGAGGAAGATATATGCAGTTTAGATGGGATCTGTAAAGACTGTACCACATGCTTTCGACACTCAGATTTGGTTCAGGATCGTCCATCTACAGCTCAATTCAGGGAGAAACTACCCTGGTTTCTAAATGCTTTGCCATCGGCTGACTGTGCAAAGGGTGGTCACGGGGCTTATACGAATAGCGTGGATTTAAAAGGGTATGAGAGTGGTGTTATACAGGCATCGGAGTTCCGTACATACCACACTCCACTTAACACACAAGGTGACTATGTTAATGCACTGCGAGCTGCTCGGGAGTTCAGTTCAAGAATATCCAATTCCTTGAAGATTGACATCTTTCCATATTCTGTCTTCTACATTTTCTTTGAGCAATATCTGAATATCTGGACAGTAGCTTTGACAAACCTTGCTATAGCAATCGGTGCTATATTTATTGTTTGCTGGTTAATCACTTCCAGTGCCTGGAGTTCAGCCATCATTGTCCTTGTGTTAGTGATGATACTCGTGGATCTCATGGGTATGATGGTGATTCTAGGCATCCAACTCAATGCAGTTTCTGTTGTGAATTTGATAATGTCAATCGGGATTGCTGTAGAATTCTGCGTGCACATATCACATGCTTTCCTGATGAGTAGTGGGGACAGAGAACATAGAGCGAGGGAGGCACTGGAAACCATGGGAGCTTCAGTTTTCAGTGGGATCACACTCACAAAACTTGTTGGAGTGATCGTGCTTTGCTTTGCAAGATCTGAGATATTTGTGGTGTATTACTTCCAAATGTACTTGGCTTTGGTTATAATCGGCTTCTTGCATGGGCTCGTTTTCTTACCTGTCATACTGAGTTTAGCTGGCCCGCCGCAGCTGAACTTGGACATCGAGCAGCAGCAGACAGACGAAGCTTCCTCTTCCCTACTGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1273

Amino Acids

139.97

Weight (kDa)

5.21

Isoelectric Point (pI)

47.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	36 - 265	2e-66	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	404 - 619	8.4e-32	NPC1, middle luminal domain
Patched	PF02460	642 - 795	3.2e-33	Patched SSD
Sterol-sensing	PF12349	663 - 813	3.7e-49	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38350 AT4G38350
fragaria_vesca	FvH4_2g16650 FvH4_2g16650 FvH4_2g16650 FvH4_2g16650
malus_domestica	MD10G1139800.v1.1
prunus_persica	Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280781
rosa_laevigata	RLG00000013029
rosa_multiflora	Rmu_sc0001197.1_g000010
rosa_roxburghii	Rroxscaffold_7G00187670
rosa_rugosa	Rorug06G0134400
rosa_samantha	Rh6AG247100 Rh6BG249400 Rh6CG250500 Rh6DG241900
rosa_wichuraiana	Rw6G021400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1832, 3698
Acc65I	GGTACC	1 cut(s)	664
AccB1I	GGYRCC	3 cut(s)	664, 1297, 1564
AccB7I	CCANNNNNTGG	1 cut(s)	3363
AccI	GTMKAC	1 cut(s)	846
AclWI	GGATC	8 cut(s)	1274, 1568, 1746, 1984, 2939, 2988, 3417, 3608
AcoI	YGGCCR	1 cut(s)	1266
AcsI	RAATTY	9 cut(s)	346, 424, 503, 1046, 1362, 1759, 2719, 3466, 3497
AcuI	CTGAAG	3 cut(s)	544, 2742, 3570
AcvI	CACGTG	1 cut(s)	524
AcyI	GRCGYC	2 cut(s)	1298, 1565
AdeI	CACNNNGTG	1 cut(s)	3164
AfaI	GTAC	9 cut(s)	256, 666, 1514, 1819, 1971, 2615, 2947, 3136, 3683
AfeI	AGCGCT	1 cut(s)	1555
AfiI	CCNNNNNNNGG	6 cut(s)	1289, 2050, 2056, 2456, 2623, 3363
AflII	CTTAAG	1 cut(s)	809
AflIII	ACRYGT	2 cut(s)	521, 1544
AjiI	CACGTC	1 cut(s)	1545
AjnI	CCWGG	5 cut(s)	607, 2049, 2410, 3017, 3362
AjuI	GAANNNNNNNTTGG	2 cut(s)	972, 1004
AloI	GAACNNNNNNTCC	4 cut(s)	1694, 1726, 2199, 2231
Alw21I	GWGCWC	3 cut(s)	363, 2699, 3510
Alw26I	GTCTC	9 cut(s)	254, 288, 387, 1142, 1396, 1535, 2092, 2568, 2609
Alw44I	GTGCAC	1 cut(s)	3506
AlwI	GGATC	8 cut(s)	1274, 1568, 1746, 1984, 2939, 2988, 3417, 3608
AlwNI	CAGNNNCTG	2 cut(s)	3363, 3458
Ama87I	CYCGRG	4 cut(s)	1600, 2382, 2571, 3192
Aor51HI	AGCGCT	1 cut(s)	1555
AoxI	GGCC	4 cut(s)	475, 1266, 2627, 3751
ApaLI	GTGCAC	1 cut(s)	3506
ApoI	RAATTY	9 cut(s)	346, 424, 503, 1046, 1362, 1759, 2719, 3466, 3497
ArsI	GACNNNNNNTTYG	2 cut(s)	3280, 3312
AseI	ATTAAT	1 cut(s)	963
Asp700I	GAANNNNTTC	4 cut(s)	1214, 1323, 1375, 2034
Asp718I	GGTACC	1 cut(s)	664
AspLEI	GCGC	6 cut(s)	116, 1300, 1436, 1556, 1567, 2305
AspS9I	GGNCC	5 cut(s)	803, 1184, 2156, 2627, 3752
AsuII	TTCGAA	1 cut(s)	2717
AvaI	CYCGRG	4 cut(s)	1600, 2382, 2571, 3192
AvaII	GGWCC	3 cut(s)	803, 1184, 2156
BaeGI	GKGCMC	2 cut(s)	1999, 3510
BalI	TGGCCA	1 cut(s)	1268
BanI	GGYRCC	3 cut(s)	664, 1297, 1564
BanII	GRGCYC	2 cut(s)	363, 3720
BarI	GAAGNNNNNNTAC	2 cut(s)	886, 918
BauI	CACGAG	2 cut(s)	2106, 3404
BbrPI	CACGTG	1 cut(s)	524
BbsI	GAAGAC	1 cut(s)	3244
Bbv12I	GWGCWC	3 cut(s)	363, 2699, 3510
BbvCI	CCTCAGC	2 cut(s)	411, 2904
BceAI	ACGGC	1 cut(s)	2916
BciT130I	CCWGG	5 cut(s)	609, 2051, 2412, 3019, 3364
BclI	TGATCA	2 cut(s)	1357, 2019
BcoDI	GTCTC	9 cut(s)	254, 288, 387, 1142, 1396, 1535, 2092, 2568, 2609
BfaI	CTAG	5 cut(s)	1077, 1302, 1578, 2045, 3434
BfmI	CTRYAG	5 cut(s)	1243, 1451, 2991, 3312, 3492
BfoI	RGCGCY	3 cut(s)	1301, 1557, 1568
BfrI	CTTAAG	1 cut(s)	809
BglII	AGATCT	2 cut(s)	2327, 3647
BmcAI	AGTACT	2 cut(s)	1514, 1819
Bme1390I	CCNGG	5 cut(s)	609, 2051, 2412, 3019, 3364
Bme18I	GGWCC	3 cut(s)	803, 1184, 2156
BmeT110I	CYCGRG	4 cut(s)	1600, 2382, 2571, 3192
BmgBI	CACGTC	1 cut(s)	1545
BmgT120I	GGNCC	5 cut(s)	803, 1184, 2156, 2627, 3752
BmiI	GGNNCC	5 cut(s)	666, 1299, 1566, 2409, 2628
BmrFI	CCNGG	5 cut(s)	609, 2051, 2412, 3019, 3364
BmsI	GCATC	7 cut(s)	651, 701, 809, 949, 1879, 3131, 3447
BpiI	GAAGAC	1 cut(s)	3244
BplI	GAGNNNNNCTC	2 cut(s)	2089, 2121
BpmI	CTGGAG	2 cut(s)	2859, 3385
Bpu10I	CCTNAGC	2 cut(s)	411, 2904
Bpu14I	TTCGAA	1 cut(s)	2717
BpuEI	CTTGAG	3 cut(s)	270, 733, 2441
BsaAI	YACGTR	1 cut(s)	524
BsaBI	GATNNNNATC	1 cut(s)	3233
BsaHI	GRCGYC	2 cut(s)	1298, 1565
BsaI	GGTCTC	3 cut(s)	288, 1142, 1396
BsaJI	CCNNGG	7 cut(s)	706, 1999, 2184, 2411, 2531, 3017, 3576
BsaXI	ACNNNNNCTCC	6 cut(s)	2199, 2229, 3573, 3603, 3615, 3645
Bsc4I	CCNNNNNNNGG	6 cut(s)	1289, 2050, 2056, 2456, 2623, 3363
Bse1I	ACTGG	6 cut(s)	1646, 2085, 2514, 2548, 3357, 3573
Bse3DI	GCAATG	3 cut(s)	250, 2517, 2772
Bse8I	GATNNNNATC	1 cut(s)	3233
BseBI	CCWGG	5 cut(s)	609, 2051, 2412, 3019, 3364
BseDI	CCNNGG	7 cut(s)	706, 1999, 2184, 2411, 2531, 3017, 3576
BseGI	GGATG	9 cut(s)	442, 642, 701, 716, 881, 940, 1778, 2159, 3438
BseJI	GATNNNNATC	1 cut(s)	3233
BseLI	CCNNNNNNNGG	6 cut(s)	1289, 2050, 2056, 2456, 2623, 3363
BseMI	GCAATG	3 cut(s)	250, 2517, 2772
BseNI	ACTGG	6 cut(s)	1646, 2085, 2514, 2548, 3357, 3573
BseRI	GAGGAG	3 cut(s)	14, 1509, 1745
BseSI	GKGCMC	2 cut(s)	1999, 3510
BsgI	GTGCAG	3 cut(s)	1602, 2124, 2881
BshFI	GGCC	4 cut(s)	477, 1268, 2629, 3753
BshNI	GGYRCC	3 cut(s)	664, 1297, 1564
BsiHKAI	GWGCWC	3 cut(s)	363, 2699, 3510
BsiHKCI	CYCGRG	4 cut(s)	1600, 2382, 2571, 3192
BslFI	GGGAC	3 cut(s)	287, 738, 3554
BslI	CCNNNNNNNGG	6 cut(s)	1289, 2050, 2056, 2456, 2623, 3363
BsmAI	GTCTC	9 cut(s)	254, 288, 387, 1142, 1396, 1535, 2092, 2568, 2609
BsmBI	CGTCTC	1 cut(s)	1535
BsmFI	GGGAC	3 cut(s)	287, 738, 3554
BsnI	GGCC	4 cut(s)	477, 1268, 2629, 3753
Bso31I	GGTCTC	3 cut(s)	288, 1142, 1396
BsoBI	CYCGRG	4 cut(s)	1600, 2382, 2571, 3192
Bsp119I	TTCGAA	1 cut(s)	2717
Bsp1286I	GDGCHC	5 cut(s)	363, 1999, 2699, 3510, 3720
Bsp1407I	TGTACA	1 cut(s)	254
Bsp19I	CCATGG	1 cut(s)	3576
BspANI	GGCC	4 cut(s)	477, 1268, 2629, 3753
BspLI	GGNNCC	5 cut(s)	666, 1299, 1566, 2409, 2628
BspMAI	CTGCAG	1 cut(s)	1455
BspPI	GGATC	8 cut(s)	1274, 1568, 1746, 1984, 2939, 2988, 3417, 3608
BspQI	GCTCTTC	1 cut(s)	1794
BspT104I	TTCGAA	1 cut(s)	2717
BspT107I	GGYRCC	3 cut(s)	664, 1297, 1564
BspTI	CTTAAG	1 cut(s)	809
BspTNI	GGTCTC	3 cut(s)	288, 1142, 1396
BsrDI	GCAATG	3 cut(s)	250, 2517, 2772
BsrGI	TGTACA	1 cut(s)	254
BsrI	ACTGG	6 cut(s)	1646, 2085, 2514, 2548, 3357, 3573
BssECI	CCNNGG	7 cut(s)	706, 1999, 2184, 2411, 2531, 3017, 3576
BssNAI	GTATAC	1 cut(s)	847
BssNI	GRCGYC	2 cut(s)	1298, 1565
BssSI	CACGAG	2 cut(s)	2106, 3404
BssT1I	CCWWGG	4 cut(s)	706, 1999, 2531, 3576
Bst1107I	GTATAC	1 cut(s)	847
Bst2BI	CACGAG	2 cut(s)	2106, 3404
Bst2UI	CCWGG	5 cut(s)	609, 2051, 2412, 3019, 3364
Bst4CI	ACNGT	9 cut(s)	164, 201, 433, 1273, 1517, 2603, 2945, 3050, 3292
Bst6I	CTCTTC	4 cut(s)	1103, 1200, 1794, 3811
BstACI	GRCGYC	2 cut(s)	1298, 1565
BstAFI	CTTAAG	1 cut(s)	809
BstAPI	GCANNNNNTGC	2 cut(s)	2519, 3323
BstAUI	TGTACA	1 cut(s)	254
BstBAI	YACGTR	1 cut(s)	524
BstBI	TTCGAA	1 cut(s)	2717
BstDSI	CCRYGG	1 cut(s)	3576
BstF5I	GGATG	9 cut(s)	442, 642, 701, 716, 881, 940, 1778, 2159, 3438
BstH2I	RGCGCY	3 cut(s)	1301, 1557, 1568
BstHHI	GCGC	6 cut(s)	116, 1300, 1436, 1556, 1567, 2305
BstMAI	GTCTC	9 cut(s)	254, 288, 387, 1142, 1396, 1535, 2092, 2568, 2609
BstNI	CCWGG	5 cut(s)	609, 2051, 2412, 3019, 3364
BstNSI	RCATGY	6 cut(s)	330, 1013, 1508, 2232, 2955, 3521
BstSCI	CCNGG	5 cut(s)	607, 2049, 2410, 3017, 3362
BstSFI	CTRYAG	5 cut(s)	1243, 1451, 2991, 3312, 3492
BstSLI	GKGCMC	2 cut(s)	1999, 3510
BstV2I	GAAGAC	1 cut(s)	3244
BstX2I	RGATCY	5 cut(s)	1976, 2327, 2931, 3409, 3647
BstXI	CCANNNNNNTGG	1 cut(s)	2170
BstYI	RGATCY	5 cut(s)	1976, 2327, 2931, 3409, 3647
BstZ17I	GTATAC	1 cut(s)	847
BsuRI	GGCC	4 cut(s)	477, 1268, 2629, 3753
BtgI	CCRYGG	1 cut(s)	3576
BtgZI	GCGATG	2 cut(s)	159, 674
BtrI	CACGTC	1 cut(s)	1545
BtsCI	GGATG	9 cut(s)	442, 642, 701, 716, 881, 940, 1778, 2159, 3438
BtsI	GCAGTG	2 cut(s)	1590, 3179
BtsIMutI	CAGTG	9 cut(s)	438, 1269, 1590, 1999, 2507, 3179, 3364, 3566, 3601
CaiI	CAGNNNCTG	2 cut(s)	3363, 3458
CfoI	GCGC	6 cut(s)	116, 1300, 1436, 1556, 1567, 2305
Cfr13I	GGNCC	5 cut(s)	803, 1184, 2156, 2627, 3752
CseI	GACGC	1 cut(s)	2736
Csp6I	GTAC	9 cut(s)	255, 665, 1513, 1818, 1970, 2614, 2946, 3135, 3682
CviQI	GTAC	9 cut(s)	255, 665, 1513, 1818, 1970, 2614, 2946, 3135, 3682
DinI	GGCGCC	2 cut(s)	1299, 1566
DraI	TTTAAA	3 cut(s)	888, 2730, 3093
DraIII	CACNNNGTG	1 cut(s)	3164
EaeI	YGGCCR	1 cut(s)	1266
Eam1104I	CTCTTC	4 cut(s)	1103, 1200, 1794, 3811
EarI	CTCTTC	4 cut(s)	1103, 1200, 1794, 3811
Ecl136II	GAGCTC	1 cut(s)	361
Eco130I	CCWWGG	4 cut(s)	706, 1999, 2531, 3576
Eco147I	AGGCCT	1 cut(s)	477
Eco24I	GRGCYC	2 cut(s)	363, 3720
Eco31I	GGTCTC	3 cut(s)	288, 1142, 1396
Eco32I	GATATC	1 cut(s)	2739
Eco47I	GGWCC	3 cut(s)	803, 1184, 2156
Eco47III	AGCGCT	1 cut(s)	1555
Eco53kI	GAGCTC	1 cut(s)	361
Eco57I	CTGAAG	3 cut(s)	544, 2742, 3570
Eco72I	CACGTG	1 cut(s)	524
Eco88I	CYCGRG	4 cut(s)	1600, 2382, 2571, 3192
EcoICRI	GAGCTC	1 cut(s)	361
EcoRI	GAATTC	2 cut(s)	2719, 3497
EcoRII	CCWGG	5 cut(s)	607, 2049, 2410, 3017, 3362
EcoRV	GATATC	1 cut(s)	2739
EcoT14I	CCWWGG	4 cut(s)	706, 1999, 2531, 3576
EcoT22I	ATGCAT	1 cut(s)	824
EcoT38I	GRGCYC	2 cut(s)	363, 3720
EgeI	GGCGCC	2 cut(s)	1299, 1566
EheI	GGCGCC	2 cut(s)	1299, 1566
ErhI	CCWWGG	4 cut(s)	706, 1999, 2531, 3576
Esp3I	CGTCTC	1 cut(s)	1535
FaqI	GGGAC	3 cut(s)	287, 738, 3554
FauI	CCCGC	1 cut(s)	3762
FauNDI	CATATG	3 cut(s)	619, 1060, 1406
FbaI	TGATCA	2 cut(s)	1357, 2019
FblI	GTMKAC	1 cut(s)	846
FokI	GGATG	9 cut(s)	449, 629, 688, 723, 888, 927, 1765, 2146, 3425
FriOI	GRGCYC	2 cut(s)	363, 3720
FspBI	CTAG	5 cut(s)	1077, 1302, 1578, 2045, 3434
GlaI	GCGC	6 cut(s)	115, 1299, 1435, 1555, 1566, 2304
GsuI	CTGGAG	2 cut(s)	2859, 3385
HaeII	RGCGCY	3 cut(s)	1301, 1557, 1568
HaeIII	GGCC	4 cut(s)	477, 1268, 2629, 3753
HgaI	GACGC	1 cut(s)	2736
HhaI	GCGC	6 cut(s)	116, 1300, 1436, 1556, 1567, 2305
Hin1I	GRCGYC	2 cut(s)	1298, 1565
Hin6I	GCGC	6 cut(s)	114, 1298, 1434, 1554, 1565, 2303
HinP1I	GCGC	6 cut(s)	114, 1298, 1434, 1554, 1565, 2303
HincII	GTYRAC	1 cut(s)	1651
HindII	GTYRAC	1 cut(s)	1651
HindIII	AAGCTT	2 cut(s)	17, 3798
Hpy166II	GTNNAC	7 cut(s)	573, 614, 847, 1651, 1867, 2853, 3508
Hpy8I	GTNNAC	7 cut(s)	573, 614, 847, 1651, 1867, 2853, 3508
HpyCH4III	ACNGT	9 cut(s)	164, 201, 433, 1273, 1517, 2603, 2945, 3050, 3292
HpyCH4IV	ACGT	7 cut(s)	396, 464, 523, 575, 849, 1544, 1656
HpySE526I	ACGT	7 cut(s)	396, 464, 523, 575, 849, 1544, 1656
Hsp92I	GRCGYC	2 cut(s)	1298, 1565
HspAI	GCGC	6 cut(s)	114, 1298, 1434, 1554, 1565, 2303
KasI	GGCGCC	2 cut(s)	1297, 1564
KpnI	GGTACC	1 cut(s)	668
Ksp22I	TGATCA	2 cut(s)	1357, 2019
LguI	GCTCTTC	1 cut(s)	1794
LmnI	GCTCC	5 cut(s)	1732, 1897, 2207, 2551, 3581
LweI	GCATC	7 cut(s)	651, 701, 809, 949, 1879, 3131, 3447
MaeI	CTAG	5 cut(s)	1077, 1302, 1578, 2045, 3434
MaeII	ACGT	7 cut(s)	396, 464, 523, 575, 849, 1544, 1656
MaeIII	GTNAC	9 cut(s)	397, 695, 1309, 1636, 1876, 2748, 2768, 3062, 3164
MflI	RGATCY	5 cut(s)	1976, 2327, 2931, 3409, 3647
MhlI	GDGCHC	5 cut(s)	363, 1999, 2699, 3510, 3720
MlsI	TGGCCA	1 cut(s)	1268
MluNI	TGGCCA	1 cut(s)	1268
Mly113I	GGCGCC	2 cut(s)	1298, 1565
MlyI	GAGTC	6 cut(s)	256, 494, 659, 1463, 1774, 2570
MmeI	TCCRAC	5 cut(s)	1963, 2185, 2825, 3466, 3601
Mox20I	TGGCCA	1 cut(s)	1268
Mph1103I	ATGCAT	1 cut(s)	824
MroXI	GAANNNNTTC	4 cut(s)	1214, 1323, 1375, 2034
MscI	TGGCCA	1 cut(s)	1268
MslI	CAYNNNNRTG	1 cut(s)	3419
Msp20I	TGGCCA	1 cut(s)	1268
MspA1I	CMGCKG	2 cut(s)	2786, 3763
MspCI	CTTAAG	1 cut(s)	809
MspR9I	CCNGG	5 cut(s)	609, 2051, 2412, 3019, 3364
MvaI	CCWGG	5 cut(s)	609, 2051, 2412, 3019, 3364
NarI	GGCGCC	2 cut(s)	1298, 1565
NcoI	CCATGG	1 cut(s)	3576
NdeI	CATATG	3 cut(s)	619, 1060, 1406
NlaIV	GGNNCC	5 cut(s)	666, 1299, 1566, 2409, 2628
NmuCI	GTSAC	6 cut(s)	397, 695, 1876, 2748, 3062, 3164
NsiI	ATGCAT	1 cut(s)	824
NspI	RCATGY	6 cut(s)	330, 1013, 1508, 2232, 2955, 3521
NspV	TTCGAA	1 cut(s)	2717
PaeI	GCATGC	2 cut(s)	330, 1508
PaeR7I	CTCGAG	2 cut(s)	2382, 2571
PceI	AGGCCT	1 cut(s)	477
PciSI	GCTCTTC	1 cut(s)	1794
PdmI	GAANNNNTTC	4 cut(s)	1214, 1323, 1375, 2034
PfeI	GAWTC	9 cut(s)	7, 782, 798, 1248, 1596, 1667, 2387, 2672, 3430
PflMI	CCANNNNNTGG	1 cut(s)	3363
PleI	GAGTC	6 cut(s)	256, 493, 658, 1463, 1773, 2570
PluTI	GGCGCC	2 cut(s)	1301, 1568
PmaCI	CACGTG	1 cut(s)	524
PmlI	CACGTG	1 cut(s)	524
PpsI	GAGTC	6 cut(s)	256, 493, 658, 1463, 1773, 2570
Ppu21I	YACGTR	1 cut(s)	524
PshBI	ATTAAT	1 cut(s)	963
PsiI	TTATAA	2 cut(s)	1832, 3698
Psp124BI	GAGCTC	1 cut(s)	363
Psp6I	CCWGG	5 cut(s)	607, 2049, 2410, 3017, 3362
PspCI	CACGTG	1 cut(s)	524
PspGI	CCWGG	5 cut(s)	607, 2049, 2410, 3017, 3362
PspN4I	GGNNCC	5 cut(s)	666, 1299, 1566, 2409, 2628
PspPI	GGNCC	5 cut(s)	803, 1184, 2156, 2627, 3752
PsrI	GAACNNNNNNTAC	2 cut(s)	1101, 1133
PstI	CTGCAG	1 cut(s)	1455
PstNI	CAGNNNCTG	2 cut(s)	3363, 3458
PsuI	RGATCY	5 cut(s)	1976, 2327, 2931, 3409, 3647
PvuII	CAGCTG	2 cut(s)	2786, 3763
RsaI	GTAC	9 cut(s)	256, 666, 1514, 1819, 1971, 2615, 2947, 3136, 3683
RsaNI	GTAC	9 cut(s)	255, 665, 1513, 1818, 1970, 2614, 2946, 3135, 3682
RseI	CAYNNNNRTG	1 cut(s)	3419
SacI	GAGCTC	1 cut(s)	363
SapI	GCTCTTC	1 cut(s)	1794
Sau96I	GGNCC	5 cut(s)	803, 1184, 2156, 2627, 3752
ScaI	AGTACT	2 cut(s)	1514, 1819
SchI	GAGTC	6 cut(s)	256, 494, 659, 1463, 1774, 2570
ScrFI	CCNGG	5 cut(s)	609, 2051, 2412, 3019, 3364
SduI	GDGCHC	5 cut(s)	363, 1999, 2699, 3510, 3720
SfaNI	GCATC	7 cut(s)	651, 701, 809, 949, 1879, 3131, 3447
SfcI	CTRYAG	5 cut(s)	1243, 1451, 2991, 3312, 3492
SfoI	GGCGCC	2 cut(s)	1299, 1566
Sfr274I	CTCGAG	2 cut(s)	2382, 2571
SfuI	TTCGAA	1 cut(s)	2717
SinI	GGWCC	3 cut(s)	803, 1184, 2156
SlaI	CTCGAG	2 cut(s)	2382, 2571
SmiMI	CAYNNNNRTG	1 cut(s)	3419
SmlI	CTYRAG	6 cut(s)	285, 748, 809, 2382, 2420, 2571
SmoI	CTYRAG	6 cut(s)	285, 748, 809, 2382, 2420, 2571
SphI	GCATGC	2 cut(s)	330, 1508
SseBI	AGGCCT	1 cut(s)	477
SspDI	GGCGCC	2 cut(s)	1297, 1564
SspI	AATATT	1 cut(s)	1324
SspMI	CTAG	5 cut(s)	1077, 1302, 1578, 2045, 3434
SstI	GAGCTC	1 cut(s)	363
StuI	AGGCCT	1 cut(s)	477
StyD4I	CCNGG	5 cut(s)	607, 2049, 2410, 3017, 3362
StyI	CCWWGG	4 cut(s)	706, 1999, 2531, 3576
TaaI	ACNGT	9 cut(s)	164, 201, 433, 1273, 1517, 2603, 2945, 3050, 3292
TaiI	ACGT	7 cut(s)	399, 467, 526, 578, 852, 1547, 1659
TaqI	TCGA	8 cut(s)	892, 1251, 2383, 2572, 2717, 2799, 2959, 3777
TaqII	GACCGA	3 cut(s)	791, 1864, 1925
TatI	WGTACW	5 cut(s)	254, 1512, 1817, 1969, 3681
TauI	GCSGC	3 cut(s)	769, 2632, 3760
TfiI	GAWTC	9 cut(s)	7, 782, 798, 1248, 1596, 1667, 2387, 2672, 3430
TscAI	CASTG	9 cut(s)	438, 1276, 1590, 1999, 2514, 3186, 3364, 3573, 3601
TseFI	GTSAC	6 cut(s)	397, 695, 1876, 2748, 3062, 3164
Tsp45I	GTSAC	6 cut(s)	397, 695, 1876, 2748, 3062, 3164
TspGWI	ACGGA	2 cut(s)	349, 3122
TspRI	CASTG	9 cut(s)	438, 1276, 1590, 1999, 2514, 3186, 3364, 3573, 3601
Van91I	CCANNNNNTGG	1 cut(s)	3363
Vha464I	CTTAAG	1 cut(s)	809
VneI	GTGCAC	1 cut(s)	3506
VpaK11BI	GGWCC	3 cut(s)	803, 1184, 2156
VspI	ATTAAT	1 cut(s)	963
XapI	RAATTY	9 cut(s)	346, 424, 503, 1046, 1362, 1759, 2719, 3466, 3497
XceI	RCATGY	6 cut(s)	330, 1013, 1508, 2232, 2955, 3521
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1173, 2790
XhoI	CTCGAG	2 cut(s)	2382, 2571
XmiI	GTMKAC	1 cut(s)	846
XmnI	GAANNNNTTC	4 cut(s)	1214, 1323, 1375, 2034
XspI	CTAG	5 cut(s)	1077, 1302, 1578, 2045, 3434
ZrmI	AGTACT	2 cut(s)	1514, 1819
Zsp2I	ATGCAT	1 cut(s)	824

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.