Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0002900

Physical Location & Seq

Genomic Coordinates Forward (+)

25175703 .. 25191661

Gene Structure 15959 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000013029

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3879 bp

ATGACGTCGTTTCTGGCTTTCCTCGCTGCTCTTTCCTTCTTACAGCTGCTCATCTTTGGACCTTCGTTGAGTGCAGAGAAGACTGTCTCGCCTGGTCTTCTCACTTCTGACGTCACTTCTCGGAAAATACACTCGGAAGACTACTGTGCAATGTATGATATATGTGGAGAACGCAGTGACGGCAAAGTCTTGAATTGCCCTTATGGTTCCCCCTCTGTGAAGCCTGAGGAGCTATTTTCTGCCAAAATCCAAAGTTTGTGTCCAACTATAAGTGGAAATGTTTGTTGCACTGAGCTTCAGTTTGAAACGTTGCGAGCACAGGTTCAACAAGCAATTCCATTTCTTGTAGGCTGTCCAGCATGCTTGAGGAATTTCCTAAATTTGTTTTGTGAGCTATCTTGCTCTCCAAAGCAGAGTCTGTTTATCAATGTGACTTCTATCTCCGAGGTTAGTGGCAATATGACTGTGGATGGGATCGACTATTACATATCTGACACTTTTGGAAAAGGACTGTACAACTCGTGTAAGGATGTTAAATTTGGTACCATGAACACACGGGCCATAGAATTTATTGGCGCTGGTGCTAAAAATTTTGAAGAATGGTTTGATTTTCTTGGTGAGAAAGCAGCCCCTGGTTTCCCTGGTTCACCTTATGCCATTGACTTCAAGTTAACCGTTCCCGAGTCATCTAATATGGAGCTCATGAACGTGTCAGTTTATTCATGTGCTGACACTTCACTAGGCTGCTCTTGTGGTGATTGCCCTTCTTCTCCTGCGTGTTCTAATTCTGAACCTCCTCCACACAAGAAAGAGCAATGCTCCATAAAAATTCTATCTATTGAGATCAAATGCATTGATTTTTCAGTAGCCATCATGTATATTCTTTTAGTTTTTGCATTCTTTGTTTGGGCCTTATTTTATCGGAAAAGAGAAAGGAGGAGAGCTGGATCTACTAAGGAACCATTGTTGAGTGCCATTAATGATGATGATATCAACCCTGTTAACTTACAAAAGGATGAAAGTTTTGCCAGAAAGGGGCATGAGGTGGATCCTCAAGTTACAGAAGAGGTCGAACTATCTTTTATTCAGGAATATATGTCAAGCTTTTTCAGGAGATATGGAATCTGGGTTTCTAGAAATCCCACTCTTGTGCTCTTTTCCTCGGTGGGGATTGTTCTTATACTCTGTATAGGTCTAGTTCGTTTCCAGGTGGAGACACGGCCAGAGAAGCTGTGGGTAGGTCCTGGCAGCAAGGCAGCGGAAGAGAAACAATTCTTTGACAGACATCTTGCTCCCTTCTACAGAATTGAGCAGCTCATTATAGCAACTGTTCCAGATTCAAAGCATGGCAAGTCACCCAGTATTGTCACAGATGACAATATTCAATTACTTTTTGACATACAAGACAAGGTTGACGGAATTCGTGCTAATTATTCTGGCTCAGCGGTAGCTTTAACTGAAATTTGCTTGAAGCCTATTGGCCAAGATTGTGCCACTCAAAGCATTTTGCAGTATTTTAAAATGGACCCTGAAAATTTTGACATCTACGGAGGAGTTGTACATGCCGAGTATTGCTTTCAGCATTATTCTTCTGCAGAAACATGTTTAAGTGCCTTTCAGGCTCCACTTGATCCAAGCACTGCCTTGGGAGGGTTCTCTGGAAACAACTATTCTGAGGCTTCTGCATTCGTAGTCACCTACCCAGTTAATAATGCAGTTGATGATGTAGGAAATGAGAATGGGAAGGCACTGGCTTGGGAGAAGGCTTTTATTCAGTTAGCAAAGGATGAACTGCTGCCAATGGTGCTGTCTCAGAATCTGACTCTCTCATTTTCGGCCGAAAGTTCAATTGAGGAAGAGTTAAAAAGAGAAAGTACTGCAGATGTTATAACCATAATCGTAAGCTATCTGGTAATGTTTCTGTATATATCTCTGACATTGGGAGATGCACCTCAAATATCTTCTCTTTACCTCTCATCCAAGGTCCTGCTTGGTTTGTCAGGAGTATTGCTTGTAATGCTTTCTGTTCTTGGATCTGTTGGATTTTTCAGTGCTGTGGGAGTTAAATCTACATTAATCATAATGGAAGTCATTCCTTTCCTTGTTTTGGCTGTTGGTGTAGATAACATGTGTATATTGGTACATGCCGTGAAACGGCAATCACTGGAGTTACCTTTAGAAAGACGAATAAGTAATGCACTGGTTGAAGTTGGCCCATCCATAACACTGGCTAGTTTGTCTGAGGTTTTGGCCTTTGCAGTGGGAAGCTTCATTCCTATGCCAGCATGCCGAGTCTTCTCCATGTTTGCAGCTCTTGCTGTTTTGCTGGACTTCTTTCTGCAAGTTACTGCATTTGTTGCATTGATATACTTTGACTTTTTGAGAGCTGAGGATGGCAGGGTTGATTGTTTTCCATGCATAAAAGTCTCGTCATCCTTTGTGGAATCCACTGAAGGAATCTATCATGGAAGAACTGGATTGTTGGCTTGCTATATGAAGGAAGTGCATGCACGTATTCTTGATTCATGGGTGGTTAAGATGGTTATCGTTGCTATCTTTGTTGGTTTTACCTGGGCAAGCATTGCATTATGTTCTAGGATTGAAACAGGTTTGGAGCAGCAGATTGTCCTTCCCCGGGACTCTTACCTCCAGGGGTATTTCAATAGTATCTCAGAGTATCTCAGGGTTGGACCACCATTATACTTTGTTGTAAAGGATTACAACTACAGTTCAGAATCAAGACATACAAACCAGTTATGCTCCATTAGCCAGTGTGAATCGAACTCTCTTTTGAATGAGATATCAAGAGCTTCTTCAACACCAGAATCAAGCTACATTGCTAAGCCAGCTGCCTCTTGGCTTGATGATTTTCTTGTATGGATATCCCCTGAGGCTTTTGGTTGCTGTCGGAAGTTTTTAAATGCTTCTTATTGTCCACCTGATGACCAGCCACCTTGCTGTTCACCTGATGATGGACCTTGTGGCCTTGGTGGAGTGTGCAAAGATTGTACAACCTGCTTTCGCCACTCAGATTTGATTGATGATCGTCCATCTACAGCACAATTTAGAGAGAAGCTTCCTTGGTTCCTTAATGCACTGCCATCCGCTGATTGCGCAAAAGGAGGCCATGGTGCTTACACTAATAGTGTGGATCTCAATGGTTATGGAAATGGCATTATAAGAGCGTCCGAGTTTCGCACCTATCACACACCACTTAACAAACAAGGTGATTATGTAAATTCAATGAGAGCAGCACGGGAGTTCAGCTCAAGAATGTCTGATTCCTTAAAGATGGATATCTTTCCATACTCGGTGTTCTACATCTTCTTTGAGCAATATCTGGATATATGGAGAATAGCTTTGATCAACATTGCTATAGCTCTTGGTGCAATCTTCATTGTCTGTCTGCTTATCACTTCCAGCTTATGGAGTTCAGCAATTCTCATACTTGTCTTGGCAATGATTGTTGTGGACCTACTGGGAGTGATGGCCATTTTGGATATCCAACTAAATGCGGTTTCTGTAGTAAATCTCATAATGTCGATCGGAATTGCTGTCGAGTTCTGTGTTCATATAACACATGCTTACTTGGTGAACCATGGAGACCGAAACCAACGAGCAAAGAAAGCTCTAACTAAAGTTGGACCTTCTGTGTTCAGTGGAATCACACTTACAAAGCTAGTTGGAGTGATTGTCCTTTGCTTCTCAAGATCAGAAATTTTTGTGGTTTATTATTTCCAAATGTACCTGGCGTTGGTTATTATTGGTTTCTTGCATGGGCTTGTATTTTTACCGGTGGTGTTGAGCATGTTTGGTCCGCTGAAGAGGCTTACAAATTTTGAGATTCAAGAAGTAGAATCTCCATCAAGCTTAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1293

Amino Acids

142.53

Weight (kDa)

5.12

Isoelectric Point (pI)

44.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	47 - 278	3.3e-65	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	423 - 614	1.1e-35	NPC1, middle luminal domain
Patched	PF02460	637 - 789	3.6e-32	Patched SSD
Sterol-sensing	PF12349	659 - 808	5.9e-46	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38350 AT4G38350
fragaria_vesca	FvH4_2g16650 FvH4_2g16650 FvH4_2g16650 FvH4_2g16650
malus_domestica	MD10G1139800.v1.1
prunus_persica	Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280781
rosa_laevigata	RLG00000013029
rosa_multiflora	Rmu_sc0001197.1_g000010
rosa_roxburghii	Rroxscaffold_7G00187670
rosa_rugosa	Rorug06G0134400
rosa_samantha	Rh6AG247100 Rh6BG249400 Rh6CG250500 Rh6DG241900
rosa_wichuraiana	Rw6G021400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1889, 3179
AasI	GACNNNNNNGTC	1 cut(s)	185
AatII	GACGTC	2 cut(s)	8, 114
Acc16I	TGCGCA	1 cut(s)	3115
Acc36I	ACCTGC	1 cut(s)	3023
Acc65I	GGTACC	1 cut(s)	542
AccB1I	GGYRCC	1 cut(s)	542
AccB7I	CCANNNNNTGG	1 cut(s)	3426
AciI	CCGC	5 cut(s)	1259, 1445, 3105, 3515, 3818
AclI	AACGTT	1 cut(s)	308
AclWI	GGATC	7 cut(s)	482, 955, 1043, 1056, 1625, 2041, 3159
AcoI	YGGCCR	4 cut(s)	1220, 1480, 1836, 3489
AcuI	CTGAAG	3 cut(s)	281, 2472, 3842
AcyI	GRCGYC	2 cut(s)	5, 111
AfaI	GTAC	7 cut(s)	515, 544, 1560, 1876, 2142, 3010, 3746
AfiI	CCNNNNNNNGG	9 cut(s)	1035, 1167, 1650, 2107, 2154, 2635, 2972, 3426, 3754
AflII	CTTAAG	1 cut(s)	3871
AflIII	ACRYGT	3 cut(s)	708, 1601, 2127
AgeI	ACCGGT	1 cut(s)	3793
AjnI	CCWGG	8 cut(s)	91, 631, 640, 1206, 1243, 2570, 2649, 3747
AleI	CACNNNNGTG	1 cut(s)	1148
Alw21I	GWGCWC	3 cut(s)	319, 702, 1155
Alw26I	GTCTC	5 cut(s)	91, 1208, 1815, 2431, 3597
AlwI	GGATC	7 cut(s)	482, 955, 1043, 1056, 1625, 2041, 3159
AlwNI	CAGNNNCTG	3 cut(s)	418, 632, 1819
Ama87I	CYCGRG	2 cut(s)	680, 2634
ArsI	GACNNNNNNTTYG	2 cut(s)	244, 276
AseI	ATTAAT	2 cut(s)	978, 2075
AsiGI	ACCGGT	1 cut(s)	3793
Asp700I	GAANNNNTTC	2 cut(s)	1271, 2091
Asp718I	GGTACC	1 cut(s)	542
AspLEI	GCGC	2 cut(s)	578, 3116
AsuC2I	CCSGG	2 cut(s)	2635, 2636
AsuHPI	GGTGA	8 cut(s)	629, 639, 767, 1347, 1687, 2955, 3239, 3604
AvaI	CYCGRG	2 cut(s)	680, 2634
AvaII	GGWCC	9 cut(s)	59, 1241, 1525, 1984, 2690, 2975, 3472, 3644, 3815
AxyI	CCTNAGG	2 cut(s)	225, 2889
BalI	TGGCCA	2 cut(s)	1482, 3491
BamHI	GGATCC	1 cut(s)	1048
BanI	GGYRCC	1 cut(s)	542
BanII	GRGCYC	1 cut(s)	702
BauI	CACGAG	1 cut(s)	520
BbsI	GAAGAC	4 cut(s)	86, 89, 144, 2287
Bbv12I	GWGCWC	3 cut(s)	319, 702, 1155
BbvCI	CCTCAGC	1 cut(s)	2388
BceAI	ACGGC	4 cut(s)	196, 1235, 2132, 2171
BciT130I	CCWGG	8 cut(s)	93, 633, 642, 1208, 1245, 2572, 2651, 3749
BclI	TGATCA	1 cut(s)	3363
BcnI	CCSGG	2 cut(s)	2635, 2636
BcoDI	GTCTC	5 cut(s)	91, 1208, 1815, 2431, 3597
BfaI	CTAG	6 cut(s)	740, 1134, 1196, 2232, 2595, 3680
BfmI	CTRYAG	7 cut(s)	1300, 1593, 1878, 2725, 3054, 3375, 3522
BfoI	RGCGCY	1 cut(s)	579
BfrI	CTTAAG	1 cut(s)	3871
BfuAI	ACCTGC	1 cut(s)	3023
BglI	GCCNNNNNGGC	1 cut(s)	1619
BlpI	GCTNAGC	2 cut(s)	1441, 2841
BmcAI	AGTACT	1 cut(s)	1876
Bme18I	GGWCC	9 cut(s)	59, 1241, 1525, 1984, 2690, 2975, 3472, 3644, 3815
BmeT110I	CYCGRG	2 cut(s)	680, 2634
BmiI	GGNNCC	7 cut(s)	208, 544, 960, 1050, 1527, 1623, 3086
BmrI	ACTGGG	3 cut(s)	1353, 1697, 3488
BmsI	GCATC	1 cut(s)	1936
BmuI	ACTGGG	3 cut(s)	1353, 1697, 3488
BpiI	GAAGAC	4 cut(s)	86, 89, 144, 2287
BplI	GAGNNNNNCTC	4 cut(s)	803, 835, 3251, 3283
BpmI	CTGGAG	2 cut(s)	2186, 2633
Bpu10I	CCTNAGC	1 cut(s)	2388
Bpu1102I	GCTNAGC	2 cut(s)	1441, 2841
BpuEI	CTTGAG	4 cut(s)	385, 1038, 3253, 3691
BpuMI	CCSGG	2 cut(s)	2635, 2636
BsaAI	YACGTR	1 cut(s)	2513
BsaBI	GATNNNNATC	2 cut(s)	2543, 3296
BsaHI	GRCGYC	2 cut(s)	5, 111
BsaI	GGTCTC	1 cut(s)	3597
BsaWI	WCCGGW	1 cut(s)	3793
BsaXI	ACNNNNNCTCC	4 cut(s)	1542, 1572, 3678, 3708
Bsc4I	CCNNNNNNNGG	9 cut(s)	1035, 1167, 1650, 2107, 2154, 2635, 2972, 3426, 3754
Bse118I	RCCGGY	1 cut(s)	3793
Bse21I	CCTNAGG	2 cut(s)	225, 2889
Bse3DI	GCAATG	6 cut(s)	156, 821, 2580, 2835, 3369, 3465
Bse8I	GATNNNNATC	2 cut(s)	2543, 3296
BseBI	CCWGG	8 cut(s)	93, 633, 642, 1208, 1245, 2572, 2651, 3749
BseGI	GGATG	9 cut(s)	475, 535, 1021, 1792, 1976, 2216, 2398, 2432, 3101
BseJI	GATNNNNATC	2 cut(s)	2543, 3296
BseLI	CCNNNNNNNGG	9 cut(s)	1035, 1167, 1650, 2107, 2154, 2635, 2972, 3426, 3754
BseMI	GCAATG	6 cut(s)	156, 821, 2580, 2835, 3369, 3465
BseRI	GAGGAG	4 cut(s)	242, 786, 952, 1566
BseX3I	CGGCCG	1 cut(s)	1836
BsgI	GTGCAG	1 cut(s)	93
Bsh1285I	CGRYCG	2 cut(s)	1839, 3546
BshNI	GGYRCC	1 cut(s)	542
BshTI	ACCGGT	1 cut(s)	3793
BsiEI	CGRYCG	2 cut(s)	1839, 3546
BsiHKAI	GWGCWC	3 cut(s)	319, 702, 1155
BsiHKCI	CYCGRG	2 cut(s)	680, 2634
BsiSI	CCGG	2 cut(s)	2635, 3794
BslFI	GGGAC	1 cut(s)	2651
BslI	CCNNNNNNNGG	9 cut(s)	1035, 1167, 1650, 2107, 2154, 2635, 2972, 3426, 3754
BsmAI	GTCTC	5 cut(s)	91, 1208, 1815, 2431, 3597
BsmFI	GGGAC	1 cut(s)	2651
BsmI	GAATGC	2 cut(s)	896, 1685
Bso31I	GGTCTC	1 cut(s)	3597
BsoBI	CYCGRG	2 cut(s)	680, 2634
Bsp1286I	GDGCHC	3 cut(s)	319, 702, 1155
Bsp1407I	TGTACA	3 cut(s)	513, 1558, 3008
Bsp1720I	GCTNAGC	2 cut(s)	1441, 2841
Bsp19I	CCATGG	2 cut(s)	3127, 3598
BspACI	CCGC	5 cut(s)	1259, 1445, 3105, 3515, 3818
BspHI	TCATGA	1 cut(s)	702
BspLI	GGNNCC	7 cut(s)	208, 544, 960, 1050, 1527, 1623, 3086
BspMAI	CTGCAG	2 cut(s)	1597, 1882
BspMI	ACCTGC	1 cut(s)	3023
BspPI	GGATC	7 cut(s)	482, 955, 1043, 1056, 1625, 2041, 3159
BspT107I	GGYRCC	1 cut(s)	542
BspTI	CTTAAG	1 cut(s)	3871
BspTNI	GGTCTC	1 cut(s)	3597
BsrDI	GCAATG	6 cut(s)	156, 821, 2580, 2835, 3369, 3465
BsrFI	RCCGGY	1 cut(s)	3793
BsrGI	TGTACA	3 cut(s)	513, 1558, 3008
BssAI	RCCGGY	1 cut(s)	3793
BssNI	GRCGYC	2 cut(s)	5, 111
BssSI	CACGAG	1 cut(s)	520
BssT1I	CCWWGG	6 cut(s)	1644, 1980, 2986, 3080, 3127, 3598
Bst2BI	CACGAG	1 cut(s)	520
Bst2UI	CCWGG	8 cut(s)	93, 633, 642, 1208, 1245, 2572, 2651, 3749
Bst4CI	ACNGT	7 cut(s)	85, 146, 466, 513, 676, 1330, 2729
Bst6I	CTCTTC	4 cut(s)	1059, 1257, 1851, 3818
BstACI	GRCGYC	2 cut(s)	5, 111
BstAFI	CTTAAG	1 cut(s)	3871
BstAPI	GCANNNNNTGC	3 cut(s)	2315, 2357, 2582
BstAUI	TGTACA	3 cut(s)	513, 1558, 3008
BstBAI	YACGTR	1 cut(s)	2513
BstC8I	GCNNGC	8 cut(s)	315, 361, 2283, 2287, 2488, 2508, 2578, 2847
BstDSI	CCRYGG	2 cut(s)	3127, 3598
BstF5I	GGATG	9 cut(s)	475, 535, 1021, 1792, 1976, 2216, 2398, 2432, 3101
BstH2I	RGCGCY	1 cut(s)	579
BstHHI	GCGC	2 cut(s)	578, 3116
BstMAI	GTCTC	5 cut(s)	91, 1208, 1815, 2431, 3597
BstMCI	CGRYCG	2 cut(s)	1839, 3546
BstNI	CCWGG	8 cut(s)	93, 633, 642, 1208, 1245, 2572, 2651, 3749
BstNSI	RCATGY	9 cut(s)	363, 1565, 1605, 2131, 2147, 2289, 2510, 3584, 3811
BstSFI	CTRYAG	7 cut(s)	1300, 1593, 1878, 2725, 3054, 3375, 3522
BstV2I	GAAGAC	4 cut(s)	86, 89, 144, 2287
BstX2I	RGATCY	4 cut(s)	947, 1048, 2033, 3151
BstXI	CCANNNNNNTGG	1 cut(s)	2227
BstYI	RGATCY	4 cut(s)	947, 1048, 2033, 3151
BstZI	CGGCCG	1 cut(s)	1836
Bsu36I	CCTNAGG	2 cut(s)	225, 2889
BtgI	CCRYGG	2 cut(s)	3127, 3598
BtsCI	GGATG	9 cut(s)	475, 535, 1021, 1792, 1976, 2216, 2398, 2432, 3101
BtsI	GCAGTG	4 cut(s)	181, 1638, 2265, 3095
BveI	ACCTGC	1 cut(s)	3023
Cac8I	GCNNGC	8 cut(s)	315, 361, 2283, 2287, 2488, 2508, 2578, 2847
CaiI	CAGNNNCTG	3 cut(s)	418, 632, 1819
CciI	TCATGA	1 cut(s)	702
CfoI	GCGC	2 cut(s)	578, 3116
Cfr10I	RCCGGY	1 cut(s)	3793
Cfr9I	CCCGGG	1 cut(s)	2634
CseI	GACGC	1 cut(s)	3174
Csp6I	GTAC	7 cut(s)	514, 543, 1559, 1875, 2141, 3009, 3745
CspAI	ACCGGT	1 cut(s)	3793
CviQI	GTAC	7 cut(s)	514, 543, 1559, 1875, 2141, 3009, 3745
DraI	TTTAAA	2 cut(s)	1519, 2919
DrdI	GACNNNNNNGTC	1 cut(s)	185
DseDI	GACNNNNNNGTC	1 cut(s)	185
EaeI	YGGCCR	4 cut(s)	1220, 1480, 1836, 3489
EagI	CGGCCG	1 cut(s)	1836
Eam1104I	CTCTTC	4 cut(s)	1059, 1257, 1851, 3818
EarI	CTCTTC	4 cut(s)	1059, 1257, 1851, 3818
Ecl136II	GAGCTC	1 cut(s)	700
EclXI	CGGCCG	1 cut(s)	1836
Eco130I	CCWWGG	6 cut(s)	1644, 1980, 2986, 3080, 3127, 3598
Eco24I	GRGCYC	1 cut(s)	702
Eco31I	GGTCTC	1 cut(s)	3597
Eco32I	GATATC	5 cut(s)	991, 2802, 2883, 3298, 3502
Eco47I	GGWCC	9 cut(s)	59, 1241, 1525, 1984, 2690, 2975, 3472, 3644, 3815
Eco52I	CGGCCG	1 cut(s)	1836
Eco53kI	GAGCTC	1 cut(s)	700
Eco57I	CTGAAG	3 cut(s)	281, 2472, 3842
Eco81I	CCTNAGG	2 cut(s)	225, 2889
Eco88I	CYCGRG	2 cut(s)	680, 2634
EcoICRI	GAGCTC	1 cut(s)	700
EcoO109I	RGGNCCY	2 cut(s)	1241, 1984
EcoRI	GAATTC	1 cut(s)	1419
EcoRII	CCWGG	8 cut(s)	91, 631, 640, 1206, 1243, 2570, 2649, 3747
EcoRV	GATATC	5 cut(s)	991, 2802, 2883, 3298, 3502
EcoT14I	CCWWGG	6 cut(s)	1644, 1980, 2986, 3080, 3127, 3598
EcoT22I	ATGCAT	2 cut(s)	854, 2420
EcoT38I	GRGCYC	1 cut(s)	702
ErhI	CCWWGG	6 cut(s)	1644, 1980, 2986, 3080, 3127, 3598
FalI	AAGNNNNNCTT	2 cut(s)	2797, 2829
FaqI	GGGAC	1 cut(s)	2651
FbaI	TGATCA	1 cut(s)	3363
FokI	GGATG	9 cut(s)	482, 542, 1028, 1799, 1963, 2203, 2405, 2419, 3088
FriOI	GRGCYC	1 cut(s)	702
FspBI	CTAG	6 cut(s)	740, 1134, 1196, 2232, 2595, 3680
FspI	TGCGCA	1 cut(s)	3115
GlaI	GCGC	2 cut(s)	577, 3115
GsuI	CTGGAG	2 cut(s)	2186, 2633
HaeII	RGCGCY	1 cut(s)	579
HapII	CCGG	2 cut(s)	2635, 3794
HgaI	GACGC	1 cut(s)	3174
HhaI	GCGC	2 cut(s)	578, 3116
Hin1I	GRCGYC	2 cut(s)	5, 111
Hin6I	GCGC	2 cut(s)	576, 3114
HinP1I	GCGC	2 cut(s)	576, 3114
HincII	GTYRAC	3 cut(s)	672, 1003, 1414
HindII	GTYRAC	3 cut(s)	672, 1003, 1414
HindIII	AAGCTT	4 cut(s)	1102, 2266, 3074, 3868
HpaI	GTTAAC	2 cut(s)	672, 1003
HpaII	CCGG	2 cut(s)	2635, 3794
HphI	GGTGA	8 cut(s)	629, 639, 767, 1347, 1687, 2955, 3239, 3604
Hpy166II	GTNNAC	8 cut(s)	647, 672, 1003, 1414, 2936, 2963, 3472, 3595
Hpy8I	GTNNAC	8 cut(s)	647, 672, 1003, 1414, 2936, 2963, 3472, 3595
Hpy99I	CGWCG	1 cut(s)	10
HpyCH4III	ACNGT	7 cut(s)	85, 146, 466, 513, 676, 1330, 2729
HpyCH4IV	ACGT	5 cut(s)	5, 111, 308, 708, 2512
HpySE526I	ACGT	5 cut(s)	5, 111, 308, 708, 2512
Hsp92I	GRCGYC	2 cut(s)	5, 111
HspAI	GCGC	2 cut(s)	576, 3114
KpnI	GGTACC	1 cut(s)	546
Ksp22I	TGATCA	1 cut(s)	3363
KspAI	GTTAAC	2 cut(s)	672, 1003
LmnI	GCTCC	7 cut(s)	229, 697, 824, 1297, 1627, 2614, 2765
LweI	GCATC	1 cut(s)	1936
MaeI	CTAG	6 cut(s)	740, 1134, 1196, 2232, 2595, 3680
MaeII	ACGT	5 cut(s)	5, 111, 308, 708, 2512
MaeIII	GTNAC	9 cut(s)	112, 176, 430, 1057, 1353, 1366, 1693, 2169, 2344
MfeI	CAATTG	1 cut(s)	1848
MflI	RGATCY	4 cut(s)	947, 1048, 2033, 3151
MhlI	GDGCHC	3 cut(s)	319, 702, 1155
MlsI	TGGCCA	2 cut(s)	1482, 3491
MluNI	TGGCCA	2 cut(s)	1482, 3491
MlyI	GAGTC	5 cut(s)	424, 692, 1816, 2301, 2633
MmeI	TCCRAC	7 cut(s)	287, 2020, 2668, 2888, 3529, 3622, 3664
Mox20I	TGGCCA	2 cut(s)	1482, 3491
Mph1103I	ATGCAT	2 cut(s)	854, 2420
MroXI	GAANNNNTTC	2 cut(s)	1271, 2091
MscI	TGGCCA	2 cut(s)	1482, 3491
MslI	CAYNNNNRTG	3 cut(s)	707, 1148, 2276
Msp20I	TGGCCA	2 cut(s)	1482, 3491
MspA1I	CMGCKG	6 cut(s)	46, 1259, 1445, 2849, 3107, 3820
MspCI	CTTAAG	1 cut(s)	3871
MspI	CCGG	2 cut(s)	2635, 3794
MunI	CAATTG	1 cut(s)	1848
Mva1269I	GAATGC	2 cut(s)	896, 1685
MvaI	CCWGG	8 cut(s)	93, 633, 642, 1208, 1245, 2572, 2651, 3749
NciI	CCSGG	2 cut(s)	2635, 2636
NcoI	CCATGG	2 cut(s)	3127, 3598
NlaIV	GGNNCC	7 cut(s)	208, 544, 960, 1050, 1527, 1623, 3086
NmeAIII	GCCGAG	2 cut(s)	1591, 2315
NmuCI	GTSAC	6 cut(s)	112, 176, 430, 1353, 1366, 1693
NsbI	TGCGCA	1 cut(s)	3115
NsiI	ATGCAT	2 cut(s)	854, 2420
NspI	RCATGY	9 cut(s)	363, 1565, 1605, 2131, 2147, 2289, 2510, 3584, 3811
OliI	CACNNNNGTG	1 cut(s)	1148
PaeI	GCATGC	3 cut(s)	363, 2289, 2510
PagI	TCATGA	1 cut(s)	702
PciI	ACATGT	2 cut(s)	1601, 2127
PctI	GAATGC	2 cut(s)	896, 1685
PdmI	GAANNNNTTC	2 cut(s)	1271, 2091
PflMI	CCANNNNNTGG	1 cut(s)	3426
PinAI	ACCGGT	1 cut(s)	3793
Ple19I	CGATCG	1 cut(s)	3546
PleI	GAGTC	5 cut(s)	423, 691, 1816, 2300, 2633
PpsI	GAGTC	5 cut(s)	423, 691, 1816, 2300, 2633
Ppu21I	YACGTR	1 cut(s)	2513
PpuMI	RGGWCCY	2 cut(s)	1241, 1984
PscI	ACATGT	2 cut(s)	1601, 2127
PshBI	ATTAAT	2 cut(s)	978, 2075
PsiI	TTATAA	2 cut(s)	1889, 3179
Psp124BI	GAGCTC	1 cut(s)	702
Psp1406I	AACGTT	1 cut(s)	308
Psp5II	RGGWCCY	2 cut(s)	1241, 1984
Psp6I	CCWGG	8 cut(s)	91, 631, 640, 1206, 1243, 2570, 2649, 3747
PspGI	CCWGG	8 cut(s)	91, 631, 640, 1206, 1243, 2570, 2649, 3747
PspN4I	GGNNCC	7 cut(s)	208, 544, 960, 1050, 1527, 1623, 3086
PspPPI	RGGWCCY	2 cut(s)	1241, 1984
PstI	CTGCAG	2 cut(s)	1597, 1882
PstNI	CAGNNNCTG	3 cut(s)	418, 632, 1819
PsuI	RGATCY	4 cut(s)	947, 1048, 2033, 3151
PvuI	CGATCG	1 cut(s)	3546
PvuII	CAGCTG	2 cut(s)	46, 2849
RsaI	GTAC	7 cut(s)	515, 544, 1560, 1876, 2142, 3010, 3746
RsaNI	GTAC	7 cut(s)	514, 543, 1559, 1875, 2141, 3009, 3745
RseI	CAYNNNNRTG	3 cut(s)	707, 1148, 2276
SacI	GAGCTC	1 cut(s)	702
ScaI	AGTACT	1 cut(s)	1876
SchI	GAGTC	5 cut(s)	424, 692, 1816, 2301, 2633
SduI	GDGCHC	3 cut(s)	319, 702, 1155
SfaNI	GCATC	1 cut(s)	1936
SfcI	CTRYAG	7 cut(s)	1300, 1593, 1878, 2725, 3054, 3375, 3522
SinI	GGWCC	9 cut(s)	59, 1241, 1525, 1984, 2690, 2975, 3472, 3644, 3815
SmaI	CCCGGG	1 cut(s)	2636
SmiMI	CAYNNNNRTG	3 cut(s)	707, 1148, 2276
SmlI	CTYRAG	5 cut(s)	364, 1053, 3268, 3706, 3871
SmoI	CTYRAG	5 cut(s)	364, 1053, 3268, 3706, 3871
SphI	GCATGC	3 cut(s)	363, 2289, 2510
SsiI	CCGC	5 cut(s)	1259, 1445, 3105, 3515, 3818
SspI	AATATT	1 cut(s)	1381
SspMI	CTAG	6 cut(s)	740, 1134, 1196, 2232, 2595, 3680
SstI	GAGCTC	1 cut(s)	702
StyI	CCWWGG	6 cut(s)	1644, 1980, 2986, 3080, 3127, 3598
TaaI	ACNGT	7 cut(s)	85, 146, 466, 513, 676, 1330, 2729
TaiI	ACGT	5 cut(s)	8, 114, 311, 711, 2515
TaqI	TCGA	5 cut(s)	477, 1071, 2780, 3542, 3558
TaqII	GACCGA	1 cut(s)	3621
TatI	WGTACW	4 cut(s)	513, 1558, 1874, 3008
TseFI	GTSAC	6 cut(s)	112, 176, 430, 1353, 1366, 1693
Tsp45I	GTSAC	6 cut(s)	112, 176, 430, 1353, 1366, 1693
TspGWI	ACGGA	2 cut(s)	1431, 1563
TspMI	CCCGGG	1 cut(s)	2634
Van91I	CCANNNNNTGG	1 cut(s)	3426
Vha464I	CTTAAG	1 cut(s)	3871
VpaK11BI	GGWCC	9 cut(s)	59, 1241, 1525, 1984, 2690, 2975, 3472, 3644, 3815
VspI	ATTAAT	2 cut(s)	978, 2075
XbaI	TCTAGA	1 cut(s)	1133
XceI	RCATGY	9 cut(s)	363, 1565, 1605, 2131, 2147, 2289, 2510, 3584, 3811
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1230, 2853
XmaI	CCCGGG	1 cut(s)	2634
XmnI	GAANNNNTTC	2 cut(s)	1271, 2091
XspI	CTAG	6 cut(s)	740, 1134, 1196, 2232, 2595, 3680
ZraI	GACGTC	2 cut(s)	6, 112
ZrmI	AGTACT	1 cut(s)	1876
Zsp2I	ATGCAT	2 cut(s)	854, 2420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.