Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0002900

Physical Location & Seq

Genomic Coordinates Forward (+)

27072146 .. 27090966

Gene Structure 18821 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00187670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3846 bp

ATGACGTCGTTTCTGGCTTTCCTCGCTGCTCTTTCCTTCTTACAGGCAAGAATATCTCATCAATTTTTCATAATTCTGCTCTTCTTTGGACCTTCGTTGAGTGCAGAGAAGACTGTCTCGCCTGGTCGTCTCACTTCTGACGTCACTTCTCGGAAAACACACTCGGAAGACTACTGTGCAATGTATGATATATGTGGAGAACGCAGTGACGGCAAAGTCTTGAATTGCCCTTATGGTTCCCCCTCTGTGAAGCCTGACGAGCTATTTTCTGCCAAAATCCAAAGTTTGTGTCCAACTATAAGTGGAAATGTTTGTTGCACTGAGCTTCAGTTTGAAACGTTGCGAGCACAGGTTCAACAAGCAATTCCATTTCTTGTAGGCTGTCCAGCATGCTTGAGGAATTTCTTAAATTTGTTTTGTGAGCTATCTTGCTCTCCAAAGCAGAGTCTGTTTATCAATGTGACTTCTATCTCCGAGGTCAGACTTCATGTTACTGCTGTTAAACTTGTTAGTGGCAATATGACTGTGGATGGGATCGACTATTACATATCTGACACTTTTGGAAAAGGACTGTACAACTCGTGTAAGGATGTTAAATTTGGTACTATGAACACACGGGCCATAGAATTTATTGGTGCTGGTGCTAAAAATTTTAAAGAATGGTTTGATTTTCTTGGTGAGAAAGCAGCCCCTGGTTTCCCTGGTTCACCTTATGCCATTGACTTCAAGTTAACAGTTCCCGAGTCATCTAATATGGAGCTCATGAACCTGTCAGTTTATTCATGTGCTGACACTTCACTAGGCTGCTCTTGTGGTGATTGCCCTTCATCTCCTGCGTGTTCTAATTCTGAACCTCCTCCACACAAGAAAGAGCAATGCTCCATAAAAATTCTATCTATTGAGATCAAATGCATTGATGTTTCAGTAGCCATCATGTATATTCTTTTAGTTTTTGCATTCTTTGTTTGGGGCTTGTTTTATCGGAAAAGAGAAAGGAGGAGAGCTGGATCTACTAAGGAACCATTGTTGAGTGCCATTGATGATGATGATATCAACCCTGTTAACTTACAAAAGGATGAAAGTTTTGCCAGAAAGGGGCATGAGGTGGATCCTCAAGTTACAGAAGAGGTTGAACTATCTTTTATTCAGGAATATATGTCAAGCTTTTTCAGGAGATATGGAATCTGGGTTTCTAGAAATCCCACTCTTGTGCTCTTTTCCTCGGTGGGGATTGTTCTTATACTCTGTATTGGTCTAGTTCGTTTCCAGCTGTGGGTAGGTCCTGGCAGCAAGGCAGCGGAGGAGAAACAATTCTTTGACAGACATCTTGCTCCCTTCTACAGAATTGAGCAGCTCATTATAGCAACTGTCCCAGATTCAAAGCACGGCGAGTCACCCAGTATTGTCACAGATGACAATATTCAATTACTTTTTGACATACAAGACAAGGTTGACGGAATTCGTGCTAATTATTCTGGCTCAGCGGTAGCTTTAACTGAAATTTGCTTGAAGCCTATTGGCCAAGATTGTGCCACTCAAAGCATTTTGCAGTATTTTAAAATGGACCCTGAAAATTTTGACATCTATGGAGGAGTTGTACATGCCGAGTACTGCTTTCAGCATTATTCTTCTGCAGAAACATGTTTAAGTGCCTTCCAGGCTCCACTTGATCCAAGCACTGCCTTGGGAGGGTTCTCTGGAAACAATTATTCTGAGGCTTCTGCATTCGTAGTCACCTACCCGGTTAATAACGCAGTTGATGATGTAGGAAATGAGAATGGGAAGGCACTGGCTTGGGAGAAGGCTTTTATTCAGTTAGCAAAGGATGAACTGCTGCCAATGGTGCTGTCTCGGAATCTGACTCTCTCATTTTCAGCCGAAAGTTCAATTGAGGAAGAGTTAAAAAGAGAAAGTACTGCAGATGTTATAACCATAATAGTAAGCTATCTGGCAATGTTTCTGTATATATCTCTGACATTGGGAGATGCACCTCAAATATCTTCTCTTTACCTCTCATCCAAGGTTGGTGTAGATAACATGTGTATATTGGTACATGCTGTCAAACGGCAATCACCGGAGTTACCTTTAGAAAGACGAATAACTCTTGCTGTTTTGCTGGACTTCTTTCTGCAAGTTACTGCATTTGTTGCATTGATATACTTTGACTTTTTGAGAGCTGAGGATGGCAGGGTTGATTGTTTTCCATGCATAAAAGTCTCGTCATCCTTTGTGGAATCCACTGAAGGAATCTATCATGGAAGAACTGGATTGTTGGCTTGCTATATGAAGGAAGTGCATGCACGTATTCTTGATTCATGGATGGTTAAGATGGTTATCGTTGCTATCTTTGTTGGTTTTACCTGGGCAAGCATTGCATTATGTTCTAGGATTGAAACAGGTTTGGAGCAGCAGATTGTCCTTCCCCGGGACTCTTACCTCCAGGGGTATTTCAATAGTATCTCAGAGTATCTCAGGGTTGGACCACCATTATACTTTGTTGTAAAGGATTACAACTACAGTTCTGGTGCTACTCTTGATTACACGCATGCTGCTTGGGGTTTTAGCTTTGCTTGCTCAGAATCAAGACATACAAACCAGTTATGCTCCATTAGCCAATGTGAATCGAACTCTCTTTTGAATGAGATATCAAGAGCTTCTTTAACACCAGAATCAAGCTACATTGCTAAGCCAGCTGCCTCTTGGCTTGATGATTTTCTTGTATGGATATCCCCCGAGGCTTTTGGTTGCTGTCGGAAGTTTTTAAATGCTTCTTATTGTCCACCTGATGACCAGGTTGGTGGTTTGTTACATTTCTTATCTGATAAGCCACGACGGTGCGGCGCCAGTTGCTGGAGGGAGGCCAGGGGTCGGACGGACCAGTCTGCAGTCGGGTGGAATCGCCGGCGACGAGGTTGCAGCGCTACAGGTGTGGTTGCAGTTGCAGGTGAGGTTGCTGCCCAGAAACCTGCAACCTCGACCTCCTCCGACCTCGATCGCTGTCCAGAAGCCGTCTGGGATGCTTCCGGCCTCCTTCTGGCCCGATTCGCGCCGCCGTCGAGGCCTAAGCGGAGCTGCAGGGCCGTCGTCCGCACTTTAGCGAAAAGTGCGGACTTTATCAAGGATGGCATTTTAATTGGTAACTCCACTGTGTATGCTGAACTGTGGTTAATACTGCTTTATAATATGATCTCTGATATTCGTTTGCTGAAGCCATTGACCAGCACATGCCACCTTGCTGTTCACCTGATGATGGACCTTGTGGCCTTGGTGGAGTGTTATGGAAATGGCATTATAAGAGCGTCCGAGTTTCGCACCTATCACACGCCACTTAACAAACAAGGTGATTATGTAAATTCAATGAGAGCAGCACGGGAGTTCAGCTCAAGAATGTCCGATTCCTTAAAGATGGATATCTTTCCATACTCGGTGTTCTACATCTTCTTTGAGCAATATCTGGATATATGGAGAATAGCTTTGATCAACATTGCTATAGCTCTTGGTGCAATCTTCCTTGTCTGTCTGCTTATCACTTCCAGCTTATGGAGTTCAGCAATTCTCATACTTGTCTTGGCAATGATTGTTGTGGACCTAATGGTGAACCATGGGGACAGAAACCAACGAGCAAAGAAAGCTCTAACTAAAGTTGGACCGTCTGTGTTCAGTGGAATCACACTTACAAAGCTAGTTGGAGTGATTGTCCTTTGCTTCTCAAGATCGGAAATTTTTGTGGTTTATTATTTCCAAATGTACCTGGCGTTGGTTCTTATTGGTTTCTTGCATGGGCTTGTATTTTTACCGGGTGGTGTTGAGCATGTTTGGTCCCCTGAAGAGGCTTACAAATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1281

Amino Acids

142.31

Weight (kDa)

5.78

Isoelectric Point (pI)

46.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	57 - 298	7.3e-64	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	436 - 627	1.7e-35	NPC1, middle luminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38350 AT4G38350
fragaria_vesca	FvH4_2g16650 FvH4_2g16650 FvH4_2g16650 FvH4_2g16650
malus_domestica	MD10G1139800.v1.1
prunus_persica	Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280781
rosa_laevigata	RLG00000013029
rosa_multiflora	Rmu_sc0001197.1_g000010
rosa_roxburghii	Rroxscaffold_7G00187670
rosa_rugosa	Rorug06G0134400
rosa_samantha	Rh6AG247100 Rh6BG249400 Rh6CG250500 Rh6DG241900
rosa_wichuraiana	Rw6G021400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1928, 3180, 3293
AarI	CACCTGC	1 cut(s)	2933
AasI	GACNNNNNNGTC	2 cut(s)	215, 2878
AatII	GACGTC	2 cut(s)	8, 144
Acc36I	ACCTGC	2 cut(s)	2933, 2974
AccB1I	GGYRCC	1 cut(s)	2840
AccB7I	CCANNNNNTGG	2 cut(s)	2850, 3540
AccII	CGCG	1 cut(s)	3047
AciI	CCGC	7 cut(s)	1298, 1484, 2838, 3050, 3067, 3088, 3107
AclI	AACGTT	1 cut(s)	338
AclWI	GGATC	5 cut(s)	542, 1015, 1103, 1116, 1664
AcoI	YGGCCR	1 cut(s)	1519
AcuI	CTGAAG	4 cut(s)	311, 2262, 3227, 3846
AcyI	GRCGYC	3 cut(s)	5, 141, 2841
AfaI	GTAC	7 cut(s)	575, 604, 1599, 1610, 1915, 2052, 3749
AfeI	AGCGCT	1 cut(s)	2920
AflIII	ACRYGT	2 cut(s)	1640, 2037
AleI	CACNNNNGTG	2 cut(s)	1208, 2833
Alw21I	GWGCWC	3 cut(s)	349, 762, 1215
Alw26I	GTCTC	4 cut(s)	121, 134, 1854, 2221
AlwI	GGATC	5 cut(s)	542, 1015, 1103, 1116, 1664
AlwNI	CAGNNNCTG	3 cut(s)	448, 692, 2850
Ama87I	CYCGRG	3 cut(s)	740, 2424, 2732
Aor51HI	AGCGCT	1 cut(s)	2920
AoxI	GGCC	8 cut(s)	618, 1519, 2859, 3025, 3036, 3059, 3078, 3261
ArsI	GACNNNNNNTTYG	2 cut(s)	274, 306
Asp700I	GAANNNNTTC	1 cut(s)	1310
AspLEI	GCGC	3 cut(s)	2843, 2921, 3049
AsuC2I	CCSGG	4 cut(s)	1742, 2425, 2426, 3798
AvaI	CYCGRG	3 cut(s)	740, 2424, 2732
AvaII	GGWCC	9 cut(s)	89, 1280, 1564, 2480, 2875, 3253, 3586, 3647, 3819
BalI	TGGCCA	1 cut(s)	1521
BamHI	GGATCC	1 cut(s)	1108
BanI	GGYRCC	1 cut(s)	2840
BanII	GRGCYC	1 cut(s)	762
BauI	CACGAG	1 cut(s)	580
BbsI	GAAGAC	2 cut(s)	116, 174
Bbv12I	GWGCWC	3 cut(s)	349, 762, 1215
BbvCI	CCTCAGC	1 cut(s)	2178
BccI	CCATC	8 cut(s)	524, 938, 2177, 2314, 2323, 3116, 3244, 3400
BceAI	ACGGC	6 cut(s)	226, 1402, 2081, 2993, 3037, 3065
BcgI	CGANNNNNNTGC	4 cut(s)	2895, 2929, 3064, 3098
BclI	TGATCA	1 cut(s)	3477
BcnI	CCSGG	4 cut(s)	1742, 2425, 2426, 3798
BcoDI	GTCTC	4 cut(s)	121, 134, 1854, 2221
BfaI	CTAG	5 cut(s)	800, 1194, 1256, 2385, 3683
BfmI	CTRYAG	8 cut(s)	1339, 1632, 1917, 2515, 2883, 2922, 3073, 3489
BfoI	RGCGCY	2 cut(s)	2844, 2922
BfuAI	ACCTGC	2 cut(s)	2933, 2974
BglI	GCCNNNNNGGC	2 cut(s)	1658, 3058
BlpI	GCTNAGC	2 cut(s)	1480, 2685
BmcAI	AGTACT	2 cut(s)	1610, 1915
Bme18I	GGWCC	9 cut(s)	89, 1280, 1564, 2480, 2875, 3253, 3586, 3647, 3819
BmeT110I	CYCGRG	3 cut(s)	740, 2424, 2732
BmiI	GGNNCC	7 cut(s)	238, 1020, 1110, 1566, 1662, 2842, 3821
BmrI	ACTGGG	1 cut(s)	1392
BmsI	GCATC	2 cut(s)	1975, 3007
BmuI	ACTGGG	1 cut(s)	1392
BpiI	GAAGAC	2 cut(s)	116, 174
BplI	GAGNNNNNCTC	4 cut(s)	863, 895, 3365, 3397
BpmI	CTGGAG	2 cut(s)	2423, 2872
Bpu10I	CCTNAGC	2 cut(s)	2178, 3063
Bpu1102I	GCTNAGC	2 cut(s)	1480, 2685
BpuEI	CTTGAG	4 cut(s)	415, 1098, 3367, 3694
BpuMI	CCSGG	4 cut(s)	1742, 2425, 2426, 3798
BsaAI	YACGTR	1 cut(s)	2303
BsaBI	GATNNNNATC	2 cut(s)	2333, 3410
BsaHI	GRCGYC	3 cut(s)	5, 141, 2841
BsaWI	WCCGGW	1 cut(s)	2074
BsaXI	ACNNNNNCTCC	4 cut(s)	1581, 1611, 3681, 3711
Bse118I	RCCGGY	1 cut(s)	2901
Bse1I	ACTGG	6 cut(s)	1398, 1794, 2269, 2596, 2844, 2878
Bse3DI	GCAATG	7 cut(s)	186, 881, 1959, 2370, 2679, 3483, 3579
Bse8I	GATNNNNATC	2 cut(s)	2333, 3410
BseJI	GATNNNNATC	2 cut(s)	2333, 3410
BseMI	GCAATG	7 cut(s)	186, 881, 1959, 2370, 2679, 3483, 3579
BseMII	CTCAG	7 cut(s)	312, 1494, 1704, 2169, 2475, 2485, 2589
BseNI	ACTGG	6 cut(s)	1398, 1794, 2269, 2596, 2844, 2878
BseRI	GAGGAG	5 cut(s)	846, 1012, 1316, 1605, 2971
BsgI	GTGCAG	1 cut(s)	123
Bsh1236I	CGCG	1 cut(s)	3047
Bsh1285I	CGRYCG	1 cut(s)	2995
BshFI	GGCC	8 cut(s)	620, 1521, 2861, 3027, 3038, 3061, 3080, 3263
BshNI	GGYRCC	1 cut(s)	2840
BsiEI	CGRYCG	1 cut(s)	2995
BsiHKAI	GWGCWC	3 cut(s)	349, 762, 1215
BsiHKCI	CYCGRG	3 cut(s)	740, 2424, 2732
BsiSI	CCGG	6 cut(s)	1742, 2075, 2425, 2902, 3024, 3797
BslFI	GGGAC	4 cut(s)	1355, 2441, 3620, 3805
BsmAI	GTCTC	4 cut(s)	121, 134, 1854, 2221
BsmBI	CGTCTC	1 cut(s)	134
BsmFI	GGGAC	4 cut(s)	1355, 2441, 3620, 3805
BsmI	GAATGC	2 cut(s)	956, 1724
BsnI	GGCC	8 cut(s)	620, 1521, 2861, 3027, 3038, 3061, 3080, 3263
BsoBI	CYCGRG	3 cut(s)	740, 2424, 2732
Bsp1286I	GDGCHC	3 cut(s)	349, 762, 1215
Bsp1407I	TGTACA	2 cut(s)	573, 1597
Bsp143I	GATC	9 cut(s)	534, 903, 1007, 1108, 1669, 2992, 3186, 3477, 3713
Bsp1720I	GCTNAGC	2 cut(s)	1480, 2685
Bsp19I	CCATGG	1 cut(s)	3601
BspACI	CCGC	7 cut(s)	1298, 1484, 2838, 3050, 3067, 3088, 3107
BspANI	GGCC	8 cut(s)	620, 1521, 2861, 3027, 3038, 3061, 3080, 3263
BspCNI	CTCAG	7 cut(s)	313, 1493, 1705, 2170, 2474, 2484, 2588
BspFNI	CGCG	1 cut(s)	3047
BspHI	TCATGA	1 cut(s)	762
BspLI	GGNNCC	7 cut(s)	238, 1020, 1110, 1566, 1662, 2842, 3821
BspMAI	CTGCAG	4 cut(s)	1636, 1921, 2887, 3077
BspMI	ACCTGC	2 cut(s)	2933, 2974
BspPI	GGATC	5 cut(s)	542, 1015, 1103, 1116, 1664
BspQI	GCTCTTC	1 cut(s)	86
BspT107I	GGYRCC	1 cut(s)	2840
BsrDI	GCAATG	7 cut(s)	186, 881, 1959, 2370, 2679, 3483, 3579
BsrFI	RCCGGY	1 cut(s)	2901
BsrGI	TGTACA	2 cut(s)	573, 1597
BsrI	ACTGG	6 cut(s)	1398, 1794, 2269, 2596, 2844, 2878
BssAI	RCCGGY	1 cut(s)	2901
BssMI	GATC	9 cut(s)	534, 903, 1007, 1108, 1669, 2992, 3186, 3477, 3713
BssNI	GRCGYC	3 cut(s)	5, 141, 2841
BssSI	CACGAG	1 cut(s)	580
BssT1I	CCWWGG	4 cut(s)	1683, 2019, 3264, 3601
Bst2BI	CACGAG	1 cut(s)	580
Bst6I	CTCTTC	4 cut(s)	86, 1119, 1890, 3822
BstACI	GRCGYC	3 cut(s)	5, 141, 2841
BstAPI	GCANNNNNTGC	2 cut(s)	2147, 2372
BstAUI	TGTACA	2 cut(s)	573, 1597
BstBAI	YACGTR	1 cut(s)	2303
BstC8I	GCNNGC	9 cut(s)	345, 391, 2278, 2298, 2368, 2547, 2572, 2691, 2903
BstDSI	CCRYGG	1 cut(s)	3601
BstFNI	CGCG	1 cut(s)	3047
BstH2I	RGCGCY	2 cut(s)	2844, 2922
BstHHI	GCGC	3 cut(s)	2843, 2921, 3049
BstKTI	GATC	9 cut(s)	537, 906, 1010, 1111, 1672, 2995, 3189, 3480, 3716
BstMAI	GTCTC	4 cut(s)	121, 134, 1854, 2221
BstMBI	GATC	9 cut(s)	534, 903, 1007, 1108, 1669, 2992, 3186, 3477, 3713
BstMCI	CGRYCG	1 cut(s)	2995
BstNSI	RCATGY	9 cut(s)	393, 1604, 1644, 2041, 2057, 2300, 2549, 3228, 3815
BstSFI	CTRYAG	8 cut(s)	1339, 1632, 1917, 2515, 2883, 2922, 3073, 3489
BstUI	CGCG	1 cut(s)	3047
BstV2I	GAAGAC	2 cut(s)	116, 174
BstX2I	RGATCY	2 cut(s)	1007, 1108
BstXI	CCANNNNNNTGG	1 cut(s)	2798
BstYI	RGATCY	2 cut(s)	1007, 1108
BsuRI	GGCC	8 cut(s)	620, 1521, 2861, 3027, 3038, 3061, 3080, 3263
BtgI	CCRYGG	1 cut(s)	3601
BtsI	GCAGTG	2 cut(s)	211, 1677
BtsIMutI	CAGTG	7 cut(s)	211, 318, 1677, 1787, 2238, 3144, 3667
BveI	ACCTGC	2 cut(s)	2933, 2974
Cac8I	GCNNGC	9 cut(s)	345, 391, 2278, 2298, 2368, 2547, 2572, 2691, 2903
CaiI	CAGNNNCTG	3 cut(s)	448, 692, 2850
CciI	TCATGA	1 cut(s)	762
CfoI	GCGC	3 cut(s)	2843, 2921, 3049
Cfr10I	RCCGGY	1 cut(s)	2901
Cfr9I	CCCGGG	1 cut(s)	2424
CseI	GACGC	1 cut(s)	3288
CsiI	ACCWGGT	1 cut(s)	2790
Csp6I	GTAC	7 cut(s)	574, 603, 1598, 1609, 1914, 2051, 3748
CviQI	GTAC	7 cut(s)	574, 603, 1598, 1609, 1914, 2051, 3748
DinI	GGCGCC	1 cut(s)	2842
DpnI	GATC	9 cut(s)	536, 905, 1009, 1110, 1671, 2994, 3188, 3479, 3715
DpnII	GATC	9 cut(s)	534, 903, 1007, 1108, 1669, 2992, 3186, 3477, 3713
DraI	TTTAAA	3 cut(s)	655, 1558, 2763
DrdI	GACNNNNNNGTC	2 cut(s)	215, 2878
DseDI	GACNNNNNNGTC	2 cut(s)	215, 2878
EaeI	YGGCCR	1 cut(s)	1519
Eam1104I	CTCTTC	4 cut(s)	86, 1119, 1890, 3822
EarI	CTCTTC	4 cut(s)	86, 1119, 1890, 3822
Ecl136II	GAGCTC	1 cut(s)	760
Eco130I	CCWWGG	4 cut(s)	1683, 2019, 3264, 3601
Eco147I	AGGCCT	1 cut(s)	3061
Eco24I	GRGCYC	1 cut(s)	762
Eco32I	GATATC	4 cut(s)	1051, 2646, 2727, 3412
Eco47I	GGWCC	9 cut(s)	89, 1280, 1564, 2480, 2875, 3253, 3586, 3647, 3819
Eco47III	AGCGCT	1 cut(s)	2920
Eco53kI	GAGCTC	1 cut(s)	760
Eco57I	CTGAAG	4 cut(s)	311, 2262, 3227, 3846
Eco88I	CYCGRG	3 cut(s)	740, 2424, 2732
EcoICRI	GAGCTC	1 cut(s)	760
EcoO109I	RGGNCCY	1 cut(s)	1280
EcoRI	GAATTC	1 cut(s)	1458
EcoRV	GATATC	4 cut(s)	1051, 2646, 2727, 3412
EcoT14I	CCWWGG	4 cut(s)	1683, 2019, 3264, 3601
EcoT22I	ATGCAT	2 cut(s)	914, 2210
EcoT38I	GRGCYC	1 cut(s)	762
EgeI	GGCGCC	1 cut(s)	2842
EheI	GGCGCC	1 cut(s)	2842
ErhI	CCWWGG	4 cut(s)	1683, 2019, 3264, 3601
Esp3I	CGTCTC	1 cut(s)	134
FalI	AAGNNNNNCTT	2 cut(s)	2641, 2673
FaqI	GGGAC	4 cut(s)	1355, 2441, 3620, 3805
FbaI	TGATCA	1 cut(s)	3477
FriOI	GRGCYC	1 cut(s)	762
FspBI	CTAG	5 cut(s)	800, 1194, 1256, 2385, 3683
GlaI	GCGC	3 cut(s)	2842, 2920, 3048
GsuI	CTGGAG	2 cut(s)	2423, 2872
HaeII	RGCGCY	2 cut(s)	2844, 2922
HaeIII	GGCC	8 cut(s)	620, 1521, 2861, 3027, 3038, 3061, 3080, 3263
HapII	CCGG	6 cut(s)	1742, 2075, 2425, 2902, 3024, 3797
HgaI	GACGC	1 cut(s)	3288
HhaI	GCGC	3 cut(s)	2843, 2921, 3049
Hin1I	GRCGYC	3 cut(s)	5, 141, 2841
Hin6I	GCGC	3 cut(s)	2841, 2919, 3047
HinP1I	GCGC	3 cut(s)	2841, 2919, 3047
HincII	GTYRAC	3 cut(s)	732, 1063, 1453
HindII	GTYRAC	3 cut(s)	732, 1063, 1453
HindIII	AAGCTT	1 cut(s)	1162
HpaI	GTTAAC	2 cut(s)	732, 1063
HpaII	CCGG	6 cut(s)	1742, 2075, 2425, 2902, 3024, 3797
Hpy166II	GTNNAC	8 cut(s)	707, 732, 1063, 1453, 2780, 3241, 3586, 3598
Hpy8I	GTNNAC	8 cut(s)	707, 732, 1063, 1453, 2780, 3241, 3586, 3598
Hpy99I	CGWCG	5 cut(s)	10, 2835, 2910, 3058, 3086
HpyCH4IV	ACGT	4 cut(s)	5, 141, 338, 2302
HpySE526I	ACGT	4 cut(s)	5, 141, 338, 2302
Hsp92I	GRCGYC	3 cut(s)	5, 141, 2841
HspAI	GCGC	3 cut(s)	2841, 2919, 3047
KasI	GGCGCC	1 cut(s)	2840
KroI	GCCGGC	1 cut(s)	2901
KroNI	GCCGGC	1 cut(s)	2903
Ksp22I	TGATCA	1 cut(s)	3477
KspAI	GTTAAC	2 cut(s)	732, 1063
Kzo9I	GATC	9 cut(s)	534, 903, 1007, 1108, 1669, 2992, 3186, 3477, 3713
LguI	GCTCTTC	1 cut(s)	86
LmnI	GCTCC	7 cut(s)	757, 884, 1336, 1666, 2404, 2609, 3069
LweI	GCATC	2 cut(s)	1975, 3007
MabI	ACCWGGT	1 cut(s)	2790
MaeI	CTAG	5 cut(s)	800, 1194, 1256, 2385, 3683
MaeII	ACGT	4 cut(s)	5, 141, 338, 2302
MalI	GATC	9 cut(s)	536, 905, 1009, 1110, 1671, 2994, 3188, 3479, 3715
MboI	GATC	9 cut(s)	534, 903, 1007, 1108, 1669, 2992, 3186, 3477, 3713
MfeI	CAATTG	1 cut(s)	1887
MflI	RGATCY	2 cut(s)	1007, 1108
MhlI	GDGCHC	3 cut(s)	349, 762, 1215
MlsI	TGGCCA	1 cut(s)	1521
MluNI	TGGCCA	1 cut(s)	1521
Mly113I	GGCGCC	1 cut(s)	2841
MlyI	GAGTC	5 cut(s)	454, 752, 1400, 1855, 2423
MmeI	TCCRAC	7 cut(s)	317, 2458, 2732, 2849, 3009, 3625, 3667
Mox20I	TGGCCA	1 cut(s)	1521
Mph1103I	ATGCAT	2 cut(s)	914, 2210
MreI	CGCCGGCG	1 cut(s)	2901
MroNI	GCCGGC	1 cut(s)	2901
MroXI	GAANNNNTTC	1 cut(s)	1310
MscI	TGGCCA	1 cut(s)	1521
MslI	CAYNNNNRTG	2 cut(s)	1208, 2833
Msp20I	TGGCCA	1 cut(s)	1521
MspA1I	CMGCKG	4 cut(s)	1270, 1298, 1484, 2693
MspI	CCGG	6 cut(s)	1742, 2075, 2425, 2902, 3024, 3797
MunI	CAATTG	1 cut(s)	1887
Mva1269I	GAATGC	2 cut(s)	956, 1724
MvnI	CGCG	1 cut(s)	3047
NaeI	GCCGGC	1 cut(s)	2903
NarI	GGCGCC	1 cut(s)	2841
NciI	CCSGG	4 cut(s)	1742, 2425, 2426, 3798
NcoI	CCATGG	1 cut(s)	3601
NdeII	GATC	9 cut(s)	534, 903, 1007, 1108, 1669, 2992, 3186, 3477, 3713
NgoMIV	GCCGGC	1 cut(s)	2901
NlaIV	GGNNCC	7 cut(s)	238, 1020, 1110, 1566, 1662, 2842, 3821
NmeAIII	GCCGAG	1 cut(s)	1630
NmuCI	GTSAC	6 cut(s)	142, 206, 460, 1392, 1405, 1732
NsiI	ATGCAT	2 cut(s)	914, 2210
NspI	RCATGY	9 cut(s)	393, 1604, 1644, 2041, 2057, 2300, 2549, 3228, 3815
OliI	CACNNNNGTG	2 cut(s)	1208, 2833
PaeI	GCATGC	3 cut(s)	393, 2300, 2549
PagI	TCATGA	1 cut(s)	762
PaqCI	CACCTGC	1 cut(s)	2933
PceI	AGGCCT	1 cut(s)	3061
PciI	ACATGT	2 cut(s)	1640, 2037
PciSI	GCTCTTC	1 cut(s)	86
PcsI	WCGNNNNNNNCGW	1 cut(s)	2905
PctI	GAATGC	2 cut(s)	956, 1724
PdiI	GCCGGC	1 cut(s)	2903
PdmI	GAANNNNTTC	1 cut(s)	1310
PflMI	CCANNNNNTGG	2 cut(s)	2850, 3540
Ple19I	CGATCG	1 cut(s)	2995
PleI	GAGTC	5 cut(s)	453, 751, 1399, 1855, 2423
PluTI	GGCGCC	1 cut(s)	2844
PpsI	GAGTC	5 cut(s)	453, 751, 1399, 1855, 2423
Ppu21I	YACGTR	1 cut(s)	2303
PpuMI	RGGWCCY	1 cut(s)	1280
PscI	ACATGT	2 cut(s)	1640, 2037
PsiI	TTATAA	3 cut(s)	1928, 3180, 3293
Psp124BI	GAGCTC	1 cut(s)	762
Psp1406I	AACGTT	1 cut(s)	338
Psp5II	RGGWCCY	1 cut(s)	1280
PspN4I	GGNNCC	7 cut(s)	238, 1020, 1110, 1566, 1662, 2842, 3821
PspPPI	RGGWCCY	1 cut(s)	1280
PstI	CTGCAG	4 cut(s)	1636, 1921, 2887, 3077
PstNI	CAGNNNCTG	3 cut(s)	448, 692, 2850
PsuI	RGATCY	2 cut(s)	1007, 1108
PvuI	CGATCG	1 cut(s)	2995
PvuII	CAGCTG	2 cut(s)	1270, 2693
RsaI	GTAC	7 cut(s)	575, 604, 1599, 1610, 1915, 2052, 3749
RsaNI	GTAC	7 cut(s)	574, 603, 1598, 1609, 1914, 2051, 3748
RseI	CAYNNNNRTG	2 cut(s)	1208, 2833
SacI	GAGCTC	1 cut(s)	762
SapI	GCTCTTC	1 cut(s)	86
Sau3AI	GATC	9 cut(s)	534, 903, 1007, 1108, 1669, 2992, 3186, 3477, 3713
ScaI	AGTACT	2 cut(s)	1610, 1915
SchI	GAGTC	5 cut(s)	454, 752, 1400, 1855, 2423
SduI	GDGCHC	3 cut(s)	349, 762, 1215
SexAI	ACCWGGT	1 cut(s)	2790
SfaNI	GCATC	2 cut(s)	1975, 3007
SfcI	CTRYAG	8 cut(s)	1339, 1632, 1917, 2515, 2883, 2922, 3073, 3489
SfoI	GGCGCC	1 cut(s)	2842
SgrAI	CRCCGGYG	1 cut(s)	2901
SinI	GGWCC	9 cut(s)	89, 1280, 1564, 2480, 2875, 3253, 3586, 3647, 3819
SmaI	CCCGGG	1 cut(s)	2426
SmiMI	CAYNNNNRTG	2 cut(s)	1208, 2833
SmlI	CTYRAG	4 cut(s)	394, 1113, 3382, 3709
SmoI	CTYRAG	4 cut(s)	394, 1113, 3382, 3709
SphI	GCATGC	3 cut(s)	393, 2300, 2549
SseBI	AGGCCT	1 cut(s)	3061
SsiI	CCGC	7 cut(s)	1298, 1484, 2838, 3050, 3067, 3088, 3107
SspDI	GGCGCC	1 cut(s)	2840
SspI	AATATT	1 cut(s)	1420
SspMI	CTAG	5 cut(s)	800, 1194, 1256, 2385, 3683
SstI	GAGCTC	1 cut(s)	762
StuI	AGGCCT	1 cut(s)	3061
StyI	CCWWGG	4 cut(s)	1683, 2019, 3264, 3601
TaiI	ACGT	4 cut(s)	8, 144, 341, 2305
TaqI	TCGA	5 cut(s)	537, 2624, 2975, 2991, 3056
TatI	WGTACW	4 cut(s)	573, 1597, 1608, 1913
TauI	GCSGC	2 cut(s)	2841, 3052
TscAI	CASTG	7 cut(s)	211, 325, 1684, 1794, 2245, 3151, 3667
TseFI	GTSAC	6 cut(s)	142, 206, 460, 1392, 1405, 1732
Tsp45I	GTSAC	6 cut(s)	142, 206, 460, 1392, 1405, 1732
TspGWI	ACGGA	2 cut(s)	1470, 2888
TspMI	CCCGGG	1 cut(s)	2424
TspRI	CASTG	7 cut(s)	211, 325, 1684, 1794, 2245, 3151, 3667
Van91I	CCANNNNNTGG	2 cut(s)	2850, 3540
VpaK11BI	GGWCC	9 cut(s)	89, 1280, 1564, 2480, 2875, 3253, 3586, 3647, 3819
XbaI	TCTAGA	1 cut(s)	1193
XceI	RCATGY	9 cut(s)	393, 1604, 1644, 2041, 2057, 2300, 2549, 3228, 3815
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2697, 3009
XmaI	CCCGGG	1 cut(s)	2424
XmnI	GAANNNNTTC	1 cut(s)	1310
XspI	CTAG	5 cut(s)	800, 1194, 1256, 2385, 3683
ZraI	GACGTC	2 cut(s)	6, 142
ZrmI	AGTACT	2 cut(s)	1610, 1915
Zsp2I	ATGCAT	2 cut(s)	914, 2210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.