Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002900

Physical Location & Seq

Genomic Coordinates Reverse (-)

22444558 .. 22459585

Gene Structure 15028 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1139800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3882 bp

ATGGAATCGTTTCTGTGTTTTGCTGCTTTTTTCCTTCTCTTTCAGGTTATCTTTTTCGCCTCTTTGATGAGTGCTGAGGAGACGGATTCACCGGCTCTTTCATTTTCGGACATCGCATCATCGGGGAAAAGGAACTCTGAAGAATATTGTGCAATGTATGATATATGTGGTGAACGCAGCGACGGAAAAGTCCTGAATTGCCCTTATGGCTCCCCATCCGTGACGCCTGATGAGCTATTTTCTGCCAAAATTCAAAGTTTGTGTCCAACAATAAGTGGGAATGTTTGTTGCACGGAGCTTCAGTTTGAAACATTACGCGCACAAGTTCAACAAGCAATTCCGTTTCTTGTAGGCTGCCCAGCATGCTTGAGGAACTTCCTAAATCTTTTTTGCGAGCTTTCTTGCTCTCCAAATCAGAGTCAGTTTATCAACGTGACCTCTGTGTCCGAGGTTAATGGAAATATGACTGTGGATGGCATTGACTTTTACATAGCTGACACTTTTGGAGAAGGGTTGTTCGACTCTTGCAAGGATGTTAAATTTGGGACTATGAACACACGGGCAATAGAATTTATTGGTGCCGGTGCTAAAAATTTTGAAGAATGGTTTGATTTTATTGGTGCAAAAGCAGATCCCGGTTTCCCTGGTTCACCTTATGCGATTGATTTTAAGCTGGTTGTTCCTGAGTCATCTGGAATGGAGCTCATGAATGTGTCGGTTTATTCATGTGGTGACACTTCACTAGGCTGCTCTTGTGGTGACTGCCCTTCATCTCCGGTGTGTTCAAATCCTGAACCTCCTCCGCCGAAGAAAAAGCCATGCTCCATTAGAATTCTATCTATTGAGGTAAAGTGCATTGAAGTTTCGGTTGCCATATTGTATATTCTCTTAGTTTTTGCATTCTTTGGTTGGAGCTTTTTTGGTCGGATGGGACAAAGGAGGGGATCTGGATCTAGCGGGGAACCATTGTTGAATGTCATTCATGATGTTGACATTGACTCTGTTAACTTGCACAAGGATGAAAGTGTTGCCACAAAGAGGCATGCTATGGATCCTCAAGTTACTGAAGGGATCCAACTAGCTCCTGTTCAGGGATTTATGTCAAACTTTTACAGGAGTTATGGAAGCTGGGTCTCCAGAAATCCCAGTCTTGTGCTGTTTTCCTCGGTGGCAATTGTTGTTATTCTGTGTGTGGGTCTAGTTCGTTTCAAGGTGGAGACACGGCCAGAGAAGCTGTGGGTAGGTCCTGGGAGCAAGGCAGCGGAAGAGAAACAATACTTTGACAGCCATCTTGCCCCCTTCTACAGAATTGAGCAGCTCATAATAGCGACTGTGCCAGATCCAAAAGATGGCAAGTCACCAAGTATTGTCACAGATGATAATATTCAGCTACTTTTTGACATACAAAACAAGGTTGATGGAATTCGTGCTAATTATTCTGACTCGGTGGTAGCTCTAACTGATATTTGCTTGACGCCTATTGGCCAAGATTGTGCCACCCAAAGCATTTTGCAGTATTTTAAGATGGACCCTGAAAACTTTGACAGCTATGGAGGGGTTGTACATGTAGAGTATTGCTTTCAGCATTTTACTTCTGCGGACACATGTTTAAGCGCGTTTCAGGCTCCACTTGATCCAAGCACTGCCTTGGGAGGATTCTCAGGAAACAACTATTCAGAGGCTTCTGCATTCGTTGTCACCTACCCAGTTAATAATGCAGCTGGTGATGTAGGAAATGAGAATGGGAAGGCATTGGCTTGGGAGAAAGCTTTTATTCAGCTAGCGAAGGAGGAACTGATGCCGATGGTTCTGTCTCGGAATCTTACTCTCTCATTCTCAGCCGAAAACTCAATTGAGGAAGAGTTAAAAAGAGAAAGTACTGCAGATGTGATAACCATATTAGTAAGCTATCTGGTAATGTTCGTTTACATATCTCTAGCCTTGGGAGATGCACCTCATTTGTCTTCTTTTTACCTCTCGTCCAAGGTCCTGCTTGGCTTGTCAGGAGTCATGCTTGTCATGCTTTCTGTCCTTGGGTCTGTTGGATTTTTCAGTGCTGTTGGAGTTAAATCTACACTAATCATAATGGAAGTCATTCCTTTCCTTGTTCTTGCTGTTGGTGTAGATAACATGTGTATACTGGTACATGCGGTGAAACGGCAACCACTGGACTTACCTTTAGAAATACGAATAAGTAATGCACTTGCTGAAGTTGGCCCATCCATAACGCTGGCTAGTTTATCTGAGATTCTGGCATTTGCAGTTGGAAGTTTCATTCCTATGCCAGCTTGTCGTGTCTTCTCCATGTTTGCTGCTCTTGCTGTTCTACTGGACTTCTTCCTGCAAGTTACTGCATTTGTTGCGCTGATATATTTTGACTTTTTGAGAGCTGAGGACAACAGGGTTGATTGTTTTCCATGCATACAAGTCGCTCCATCGTCTGAGGGAACTAGTGAAGGAATTTATCATAGAAGAGCTGGATTGCTGACCCGCTATATGAAGGAAGTACATGCACGCATTCTTGGATATTGGGTGATTAAAATGGTTGTTGTTGCTGTCTTTCTTGCTTTCACCTTGGCAAGCATTACATTATGTACTAGTATTGAACCTGGTTTGGAACAGAAGATTGCCCTTCCCCGGGATTCTTACCTTCAGGGGTACTTCAATAACATTTCAGAGCATCTTAGGATTGGACCACCATTATATTTTGTTGTCAAGGATTACAACTACAGCTTGGAATCAAGACATACAAACCAGTTATGCTCCATCAGTAAATGTGACTCCAACTCCCTTTTAAATGAGATATCCAGAGCATCTTCAACACCGGAATCAAGCTACATTGCTAAGCCAGCTGCCTCTTGGCTTGATGATTTTCTTGTATGGATTTCACCTGAGGCTTTTGGTTGCTGTCGGAAGTATTTAAATGGTTCTTATTGTCCCCCTGATGATCAGCCACCTTGTTGTTCACCTGATGAAGGTCCTTGTGGCCTTGGTGGAGTTTGCAAAGATTGTACAACATGCTTTCGGCACTCAGATTTGGTCAATGATCGTCCATCTACAGCACAGTTTGGAGAGAAGCTTCCATGGTTCCTAAATGCACTGCCTTCTGCTGATTGTGCAAAAGGTGGCCATGGTGCTTACACTAATAGTGTGGATCTAAAAGGTTATGGGAGTGGTGTTATAAGTGCATCTGAGTTCCGTACCTATCACACACCACTTAACGCACAAGGTGATTATGTCAATTCAATACGAGCTGCGCGGGAGTTCAGCTCAAGAATGTCTGATTCTTTAAAGATGGATATCTTTCCGTACTCGGTGTTCTATATCTTCTTTGAGCAATATCTGGATATATGGAGGGTAGCTTTGATCAACATTGCTATTGCACTTGGTGCAATCTTCATCGTTTGTCTGCTCATGACATCCAGTGTATGGAGCTCAGCTATCATTTTACTTGTACTGGCAATGATTGTTGTGGACCTCATGGGTGTAATGGCCATTTTCAATATCCAACTAAACGCAGTCTCTGTTGTAAATCTTGTAATGTCAATTGGAATCGCTGTTGAGTTCTGTGTCCATATAGTGCATGCTTTCTTGGTGAGTCATGGCAACAGGAGCCAACGAGCAACGAAAGCTCTAAGTAGAGTGGGACCTTCTGTGTTCAGTGGGATTACACTTACAAAGCTGGTTGGAGTAATTGTCCTTGCCTTCTCAAGATCAGAAATTTTTGTGGTTTACTATTTCCGAATGTACCTGGCATTGGTTCTTATTGGTTTCTTGCATGGGCTCGTCTTTTTGCCGGTGATATTGAGCTTGTTTGGTCCACCGAATAGGCACGCAAGTATTGATATGCAAGAAGTTGAATCTTCACCGGTGTTGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1294

Amino Acids

141.55

Weight (kDa)

5.03

Isoelectric Point (pI)

44.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	48 - 279	1e-65	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	424 - 615	2.8e-36	NPC1, middle luminal domain
Patched	PF02460	638 - 790	2.7e-30	Patched SSD
Sterol-sensing	PF12349	660 - 809	1.3e-46	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38350 AT4G38350
fragaria_vesca	FvH4_2g16650 FvH4_2g16650 FvH4_2g16650 FvH4_2g16650
malus_domestica	MD10G1139800.v1.1
prunus_persica	Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280781
rosa_laevigata	RLG00000013029
rosa_multiflora	Rmu_sc0001197.1_g000010
rosa_roxburghii	Rroxscaffold_7G00187670
rosa_rugosa	Rorug06G0134400
rosa_samantha	Rh6AG247100 Rh6BG249400 Rh6CG250500 Rh6DG241900
rosa_wichuraiana	Rw6G021400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3182
AasI	GACNNNNNNGTC	2 cut(s)	188, 442
AccB1I	GGYRCC	1 cut(s)	578
AccB7I	CCANNNNNTGG	2 cut(s)	1349, 3429
AccI	GTMKAC	1 cut(s)	2137
AccII	CGCG	3 cut(s)	318, 1616, 3259
AciI	CCGC	7 cut(s)	803, 957, 1262, 1598, 2150, 2491, 3259
AcoI	YGGCCR	4 cut(s)	1223, 1483, 3127, 3492
AcsI	RAATTY	8 cut(s)	249, 539, 569, 592, 831, 1422, 2460, 3720
AcuI	CTGAAG	5 cut(s)	159, 284, 1086, 2229, 2636
AcyI	GRCGYC	2 cut(s)	224, 1475
AdeI	CACNNNGTG	2 cut(s)	3230, 3389
AfiI	CCNNNNNNNGG	8 cut(s)	1038, 1091, 1349, 2637, 2638, 2975, 3429, 3757
AflIII	ACRYGT	3 cut(s)	1564, 1604, 2130
AgeI	ACCGGT	1 cut(s)	3868
AhlI	ACTAGT	2 cut(s)	2450, 2597
AjnI	CCWGG	4 cut(s)	643, 1246, 2608, 3750
Alw21I	GWGCWC	2 cut(s)	705, 3437
Alw26I	GTCTC	5 cut(s)	74, 1138, 1211, 1818, 3526
AlwNI	CAGNNNCTG	1 cut(s)	3524
Ama87I	CYCGRG	1 cut(s)	2637
AoxI	GGCC	6 cut(s)	1223, 1483, 2215, 2986, 3127, 3492
ApoI	RAATTY	8 cut(s)	249, 539, 569, 592, 831, 1422, 2460, 3720
ArsI	GACNNNNNNTTYG	2 cut(s)	247, 279
AsiGI	ACCGGT	1 cut(s)	3868
Asp700I	GAANNNNTTC	2 cut(s)	9, 2094
AspLEI	GCGC	4 cut(s)	320, 1616, 2365, 3259
AspS9I	GGNCC	9 cut(s)	1244, 1528, 1987, 2216, 2693, 2978, 3475, 3647, 3818
AsuC2I	CCSGG	3 cut(s)	636, 2638, 2639
AsuNHI	GCTAGC	1 cut(s)	1780
AvaI	CYCGRG	1 cut(s)	2637
AvaII	GGWCC	8 cut(s)	1244, 1528, 1987, 2693, 2978, 3475, 3647, 3818
AxyI	CCTNAGG	1 cut(s)	2892
BalI	TGGCCA	3 cut(s)	1485, 3129, 3494
BamHI	GGATCC	2 cut(s)	1051, 1071
BanI	GGYRCC	1 cut(s)	578
BanII	GRGCYC	3 cut(s)	705, 3437, 3786
BbsI	GAAGAC	2 cut(s)	1956, 2290
Bbv12I	GWGCWC	2 cut(s)	705, 3437
BbvCI	CCTCAGC	2 cut(s)	75, 2391
BceAI	ACGGC	2 cut(s)	1238, 2174
BcgI	CGANNNNNNTGC	2 cut(s)	2410, 2444
BciT130I	CCWGG	4 cut(s)	645, 1248, 2610, 3752
BclI	TGATCA	2 cut(s)	2947, 3366
BcnI	CCSGG	3 cut(s)	636, 2638, 2639
BcoDI	GTCTC	5 cut(s)	74, 1138, 1211, 1818, 3526
BcuI	ACTAGT	2 cut(s)	2450, 2597
BfaI	CTAG	9 cut(s)	743, 954, 1079, 1199, 1781, 1937, 2235, 2451, 2598
BfmI	CTRYAG	4 cut(s)	1303, 1881, 2728, 3057
BglI	GCCNNNNNGGC	1 cut(s)	207
BlpI	GCTNAGC	2 cut(s)	2844, 3436
BmcAI	AGTACT	1 cut(s)	1879
Bme1390I	CCNGG	7 cut(s)	636, 645, 1248, 2610, 2638, 2639, 3752
Bme18I	GGWCC	8 cut(s)	1244, 1528, 1987, 2693, 2978, 3475, 3647, 3818
BmeT110I	CYCGRG	1 cut(s)	2637
BmgT120I	GGNCC	9 cut(s)	1244, 1528, 1987, 2216, 2693, 2978, 3475, 3647, 3818
BmrFI	CCNGG	7 cut(s)	636, 645, 1248, 2610, 2638, 2639, 3752
BmrI	ACTGGG	2 cut(s)	1140, 1700
BmsI	GCATC	6 cut(s)	125, 1788, 1939, 2689, 2822, 3197
BmtI	GCTAGC	1 cut(s)	1784
BmuI	ACTGGG	2 cut(s)	1140, 1700
BpiI	GAAGAC	2 cut(s)	1956, 2290
BplI	GAGNNNNNCTC	2 cut(s)	3254, 3286
BpmI	CTGGAG	1 cut(s)	1120
Bpu10I	CCTNAGC	2 cut(s)	75, 2391
Bpu1102I	GCTNAGC	2 cut(s)	2844, 3436
BpuEI	CTTGAG	4 cut(s)	388, 1041, 3256, 3694
BpuMI	CCSGG	3 cut(s)	636, 2638, 2639
BsaBI	GATNNNNATC	2 cut(s)	949, 3299
BsaHI	GRCGYC	2 cut(s)	224, 1475
BsaI	GGTCTC	1 cut(s)	1138
BsaWI	WCCGGW	3 cut(s)	775, 2824, 3868
BsaXI	ACNNNNNCTCC	8 cut(s)	287, 317, 1545, 1575, 2771, 2801, 3681, 3711
Bsc4I	CCNNNNNNNGG	8 cut(s)	1038, 1091, 1349, 2637, 2638, 2975, 3429, 3757
Bse118I	RCCGGY	4 cut(s)	91, 581, 3796, 3868
Bse1I	ACTGG	8 cut(s)	1146, 1706, 2145, 2172, 2334, 2755, 3423, 3462
Bse21I	CCTNAGG	1 cut(s)	2892
Bse3DI	GCAATG	4 cut(s)	159, 2838, 3372, 3468
Bse8I	GATNNNNATC	2 cut(s)	949, 3299
BseBI	CCWGG	4 cut(s)	645, 1248, 2610, 3752
BseGI	GGATG	7 cut(s)	215, 478, 538, 933, 1024, 2219, 3419
BseJI	GATNNNNATC	2 cut(s)	949, 3299
BseLI	CCNNNNNNNGG	8 cut(s)	1038, 1091, 1349, 2637, 2638, 2975, 3429, 3757
BseMI	GCAATG	4 cut(s)	159, 2838, 3372, 3468
BseNI	ACTGG	8 cut(s)	1146, 1706, 2145, 2172, 2334, 2755, 3423, 3462
BseRI	GAGGAG	2 cut(s)	92, 789
BseYI	CCCAGC	2 cut(s)	358, 1128
Bsh1236I	CGCG	3 cut(s)	318, 1616, 3259
BshFI	GGCC	6 cut(s)	1225, 1485, 2217, 2988, 3129, 3494
BshNI	GGYRCC	1 cut(s)	578
BshTI	ACCGGT	1 cut(s)	3868
BsiHKAI	GWGCWC	2 cut(s)	705, 3437
BsiHKCI	CYCGRG	1 cut(s)	2637
BsiSI	CCGG	8 cut(s)	92, 582, 636, 776, 2638, 2825, 3797, 3869
BslFI	GGGAC	4 cut(s)	559, 945, 2922, 3660
BslI	CCNNNNNNNGG	8 cut(s)	1038, 1091, 1349, 2637, 2638, 2975, 3429, 3757
BsmAI	GTCTC	5 cut(s)	74, 1138, 1211, 1818, 3526
BsmBI	CGTCTC	1 cut(s)	74
BsmFI	GGGAC	4 cut(s)	559, 945, 2922, 3660
BsmI	GAATGC	3 cut(s)	899, 1688, 2517
BsnI	GGCC	6 cut(s)	1225, 1485, 2217, 2988, 3129, 3494
Bso31I	GGTCTC	1 cut(s)	1138
BsoBI	CYCGRG	1 cut(s)	2637
Bsp1286I	GDGCHC	3 cut(s)	705, 3437, 3786
Bsp1407I	TGTACA	2 cut(s)	1561, 3011
Bsp1720I	GCTNAGC	2 cut(s)	2844, 3436
Bsp19I	CCATGG	2 cut(s)	3083, 3130
BspACI	CCGC	7 cut(s)	803, 957, 1262, 1598, 2150, 2491, 3259
BspANI	GGCC	6 cut(s)	1225, 1485, 2217, 2988, 3129, 3494
BspFNI	CGCG	3 cut(s)	318, 1616, 3259
BspHI	TCATGA	3 cut(s)	705, 982, 3414
BspMAI	CTGCAG	1 cut(s)	1885
BspOI	GCTAGC	1 cut(s)	1784
BspQI	GCTCTTC	1 cut(s)	2467
BspT107I	GGYRCC	1 cut(s)	578
BspTNI	GGTCTC	1 cut(s)	1138
BsrDI	GCAATG	4 cut(s)	159, 2838, 3372, 3468
BsrFI	RCCGGY	4 cut(s)	91, 581, 3796, 3868
BsrGI	TGTACA	2 cut(s)	1561, 3011
BsrI	ACTGG	8 cut(s)	1146, 1706, 2145, 2172, 2334, 2755, 3423, 3462
BssAI	RCCGGY	4 cut(s)	91, 581, 3796, 3868
BssNAI	GTATAC	1 cut(s)	2138
BssNI	GRCGYC	2 cut(s)	224, 1475
BssT1I	CCWWGG	8 cut(s)	1647, 1941, 1983, 2032, 2574, 2989, 3083, 3130
Bst1107I	GTATAC	1 cut(s)	2138
Bst2UI	CCWGG	4 cut(s)	645, 1248, 2610, 3752
Bst4CI	ACNGT	3 cut(s)	469, 1333, 3066
Bst6I	CTCTTC	3 cut(s)	1260, 1854, 2467
BstACI	GRCGYC	2 cut(s)	224, 1475
BstAPI	GCANNNNNTGC	2 cut(s)	2360, 3389
BstAUI	TGTACA	2 cut(s)	1561, 3011
BstDSI	CCRYGG	2 cut(s)	3083, 3130
BstENI	CCTNNNNNAGG	1 cut(s)	2973
BstF5I	GGATG	7 cut(s)	215, 478, 538, 933, 1024, 2219, 3419
BstFNI	CGCG	3 cut(s)	318, 1616, 3259
BstHHI	GCGC	4 cut(s)	320, 1616, 2365, 3259
BstMAI	GTCTC	5 cut(s)	74, 1138, 1211, 1818, 3526
BstNI	CCWGG	4 cut(s)	645, 1248, 2610, 3752
BstNSI	RCATGY	9 cut(s)	366, 1046, 1568, 1608, 2134, 2150, 2513, 3021, 3587
BstSCI	CCNGG	7 cut(s)	634, 643, 1246, 2608, 2636, 2637, 3750
BstSFI	CTRYAG	4 cut(s)	1303, 1881, 2728, 3057
BstUI	CGCG	3 cut(s)	318, 1616, 3259
BstV2I	GAAGAC	2 cut(s)	1956, 2290
BstX2I	RGATCY	7 cut(s)	631, 944, 950, 1051, 1071, 1339, 3154
BstXI	CCANNNNNNTGG	1 cut(s)	2230
BstYI	RGATCY	7 cut(s)	631, 944, 950, 1051, 1071, 1339, 3154
BstZ17I	GTATAC	1 cut(s)	2138
Bsu36I	CCTNAGG	1 cut(s)	2892
BsuRI	GGCC	6 cut(s)	1225, 1485, 2217, 2988, 3129, 3494
BtgI	CCRYGG	2 cut(s)	3083, 3130
BtgZI	GCGATG	1 cut(s)	97
BtsCI	GGATG	7 cut(s)	215, 478, 538, 933, 1024, 2219, 3419
BtsI	GCAGTG	2 cut(s)	1641, 3098
BtsIMutI	CAGTG	6 cut(s)	1641, 2059, 2165, 3098, 3430, 3667
CaiI	CAGNNNCTG	1 cut(s)	3524
CciI	TCATGA	3 cut(s)	705, 982, 3414
CfoI	GCGC	4 cut(s)	320, 1616, 2365, 3259
Cfr10I	RCCGGY	4 cut(s)	91, 581, 3796, 3868
Cfr13I	GGNCC	9 cut(s)	1244, 1528, 1987, 2216, 2693, 2978, 3475, 3647, 3818
Cfr9I	CCCGGG	1 cut(s)	2637
CseI	GACGC	2 cut(s)	232, 1483
CsiI	ACCWGGT	1 cut(s)	2608
CspAI	ACCGGT	1 cut(s)	3868
DraI	TTTAAA	3 cut(s)	2796, 2922, 3291
DraIII	CACNNNGTG	2 cut(s)	3230, 3389
DrdI	GACNNNNNNGTC	2 cut(s)	188, 442
DseDI	GACNNNNNNGTC	2 cut(s)	188, 442
EaeI	YGGCCR	4 cut(s)	1223, 1483, 3127, 3492
Eam1104I	CTCTTC	3 cut(s)	1260, 1854, 2467
EarI	CTCTTC	3 cut(s)	1260, 1854, 2467
EciI	GGCGGA	1 cut(s)	792
Ecl136II	GAGCTC	2 cut(s)	703, 3435
Eco130I	CCWWGG	8 cut(s)	1647, 1941, 1983, 2032, 2574, 2989, 3083, 3130
Eco24I	GRGCYC	3 cut(s)	705, 3437, 3786
Eco31I	GGTCTC	1 cut(s)	1138
Eco32I	GATATC	2 cut(s)	2805, 3301
Eco47I	GGWCC	8 cut(s)	1244, 1528, 1987, 2693, 2978, 3475, 3647, 3818
Eco53kI	GAGCTC	2 cut(s)	703, 3435
Eco57I	CTGAAG	5 cut(s)	159, 284, 1086, 2229, 2636
Eco81I	CCTNAGG	1 cut(s)	2892
Eco88I	CYCGRG	1 cut(s)	2637
EcoICRI	GAGCTC	2 cut(s)	703, 3435
EcoNI	CCTNNNNNAGG	1 cut(s)	2973
EcoO109I	RGGNCCY	4 cut(s)	1244, 1987, 2978, 3647
EcoRI	GAATTC	2 cut(s)	831, 1422
EcoRII	CCWGG	4 cut(s)	643, 1246, 2608, 3750
EcoRV	GATATC	2 cut(s)	2805, 3301
EcoT14I	CCWWGG	8 cut(s)	1647, 1941, 1983, 2032, 2574, 2989, 3083, 3130
EcoT22I	ATGCAT	1 cut(s)	2423
EcoT38I	GRGCYC	3 cut(s)	705, 3437, 3786
ErhI	CCWWGG	8 cut(s)	1647, 1941, 1983, 2032, 2574, 2989, 3083, 3130
Esp3I	CGTCTC	1 cut(s)	74
FaqI	GGGAC	4 cut(s)	559, 945, 2922, 3660
FauI	CCCGC	3 cut(s)	950, 2498, 3252
FbaI	TGATCA	2 cut(s)	2947, 3366
FblI	GTMKAC	1 cut(s)	2137
FokI	GGATG	7 cut(s)	202, 485, 545, 940, 1031, 2206, 3406
FriOI	GRGCYC	3 cut(s)	705, 3437, 3786
FspBI	CTAG	9 cut(s)	743, 954, 1079, 1199, 1781, 1937, 2235, 2451, 2598
GlaI	GCGC	4 cut(s)	319, 1615, 2364, 3258
GsaI	CCCAGC	2 cut(s)	362, 1132
GsuI	CTGGAG	1 cut(s)	1120
HaeIII	GGCC	6 cut(s)	1225, 1485, 2217, 2988, 3129, 3494
HapII	CCGG	8 cut(s)	92, 582, 636, 776, 2638, 2825, 3797, 3869
HgaI	GACGC	2 cut(s)	232, 1483
HhaI	GCGC	4 cut(s)	320, 1616, 2365, 3259
Hin1I	GRCGYC	2 cut(s)	224, 1475
Hin6I	GCGC	4 cut(s)	318, 1614, 2363, 3257
HinP1I	GCGC	4 cut(s)	318, 1614, 2363, 3257
HincII	GTYRAC	2 cut(s)	991, 1006
HindII	GTYRAC	2 cut(s)	991, 1006
HindIII	AAGCTT	2 cut(s)	1767, 3077
HpaI	GTTAAC	1 cut(s)	1006
HpaII	CCGG	8 cut(s)	92, 582, 636, 776, 2638, 2825, 3797, 3869
Hpy99I	CGWCG	1 cut(s)	185
HpyCH4III	ACNGT	3 cut(s)	469, 1333, 3066
HpyCH4IV	ACGT	1 cut(s)	432
HpySE526I	ACGT	1 cut(s)	432
Hsp92I	GRCGYC	2 cut(s)	224, 1475
HspAI	GCGC	4 cut(s)	318, 1614, 2363, 3257
Ksp22I	TGATCA	2 cut(s)	2947, 3366
KspAI	GTTAAC	1 cut(s)	1006
LguI	GCTCTTC	1 cut(s)	2467
LweI	GCATC	6 cut(s)	125, 1788, 1939, 2689, 2822, 3197
MabI	ACCWGGT	1 cut(s)	2608
MaeI	CTAG	9 cut(s)	743, 954, 1079, 1199, 1781, 1937, 2235, 2451, 2598
MaeII	ACGT	1 cut(s)	432
MfeI	CAATTG	3 cut(s)	1173, 1851, 3546
MflI	RGATCY	7 cut(s)	631, 944, 950, 1051, 1071, 1339, 3154
MhlI	GDGCHC	3 cut(s)	705, 3437, 3786
MlsI	TGGCCA	3 cut(s)	1485, 3129, 3494
MluNI	TGGCCA	3 cut(s)	1485, 3129, 3494
MlyI	GAGTC	8 cut(s)	427, 515, 695, 992, 1436, 2016, 2774, 3607
Mox20I	TGGCCA	3 cut(s)	1485, 3129, 3494
Mph1103I	ATGCAT	1 cut(s)	2423
MroXI	GAANNNNTTC	2 cut(s)	9, 2094
MscI	TGGCCA	3 cut(s)	1485, 3129, 3494
MslI	CAYNNNNRTG	3 cut(s)	710, 1017, 2279
Msp20I	TGGCCA	3 cut(s)	1485, 3129, 3494
MspA1I	CMGCKG	3 cut(s)	1262, 1721, 2852
MspI	CCGG	8 cut(s)	92, 582, 636, 776, 2638, 2825, 3797, 3869
MspR9I	CCNGG	7 cut(s)	636, 645, 1248, 2610, 2638, 2639, 3752
MunI	CAATTG	3 cut(s)	1173, 1851, 3546
Mva1269I	GAATGC	3 cut(s)	899, 1688, 2517
MvaI	CCWGG	4 cut(s)	645, 1248, 2610, 3752
MvnI	CGCG	3 cut(s)	318, 1616, 3259
NciI	CCSGG	3 cut(s)	636, 2638, 2639
NcoI	CCATGG	2 cut(s)	3083, 3130
NheI	GCTAGC	1 cut(s)	1780
NmuCI	GTSAC	8 cut(s)	220, 433, 731, 758, 1356, 1369, 1696, 2777
NsiI	ATGCAT	1 cut(s)	2423
NspI	RCATGY	9 cut(s)	366, 1046, 1568, 1608, 2134, 2150, 2513, 3021, 3587
PaeI	GCATGC	3 cut(s)	366, 1046, 3587
PagI	TCATGA	3 cut(s)	705, 982, 3414
PciI	ACATGT	3 cut(s)	1564, 1604, 2130
PciSI	GCTCTTC	1 cut(s)	2467
PctI	GAATGC	3 cut(s)	899, 1688, 2517
PdmI	GAANNNNTTC	2 cut(s)	9, 2094
PflMI	CCANNNNNTGG	2 cut(s)	1349, 3429
PinAI	ACCGGT	1 cut(s)	3868
PleI	GAGTC	8 cut(s)	426, 515, 694, 992, 1436, 2015, 2774, 3606
PpsI	GAGTC	8 cut(s)	426, 515, 694, 992, 1436, 2015, 2774, 3606
PpuMI	RGGWCCY	4 cut(s)	1244, 1987, 2978, 3647
PscI	ACATGT	3 cut(s)	1564, 1604, 2130
PsiI	TTATAA	1 cut(s)	3182
Psp124BI	GAGCTC	2 cut(s)	705, 3437
Psp5II	RGGWCCY	4 cut(s)	1244, 1987, 2978, 3647
Psp6I	CCWGG	4 cut(s)	643, 1246, 2608, 3750
PspFI	CCCAGC	2 cut(s)	358, 1128
PspGI	CCWGG	4 cut(s)	643, 1246, 2608, 3750
PspPI	GGNCC	9 cut(s)	1244, 1528, 1987, 2216, 2693, 2978, 3475, 3647, 3818
PspPPI	RGGWCCY	4 cut(s)	1244, 1987, 2978, 3647
PsrI	GAACNNNNNNTAC	2 cut(s)	3302, 3334
PstI	CTGCAG	1 cut(s)	1885
PstNI	CAGNNNCTG	1 cut(s)	3524
PsuI	RGATCY	7 cut(s)	631, 944, 950, 1051, 1071, 1339, 3154
PvuII	CAGCTG	2 cut(s)	1721, 2852
RseI	CAYNNNNRTG	3 cut(s)	710, 1017, 2279
SacI	GAGCTC	2 cut(s)	705, 3437
SapI	GCTCTTC	1 cut(s)	2467
Sau96I	GGNCC	9 cut(s)	1244, 1528, 1987, 2216, 2693, 2978, 3475, 3647, 3818
ScaI	AGTACT	1 cut(s)	1879
SchI	GAGTC	8 cut(s)	427, 515, 695, 992, 1436, 2016, 2774, 3607
ScrFI	CCNGG	7 cut(s)	636, 645, 1248, 2610, 2638, 2639, 3752
SduI	GDGCHC	3 cut(s)	705, 3437, 3786
SexAI	ACCWGGT	1 cut(s)	2608
SfaNI	GCATC	6 cut(s)	125, 1788, 1939, 2689, 2822, 3197
SfcI	CTRYAG	4 cut(s)	1303, 1881, 2728, 3057
SgrAI	CRCCGGYG	1 cut(s)	3868
SinI	GGWCC	8 cut(s)	1244, 1528, 1987, 2693, 2978, 3475, 3647, 3818
SmaI	CCCGGG	1 cut(s)	2639
SmiI	ATTTAAAT	1 cut(s)	2922
SmiMI	CAYNNNNRTG	3 cut(s)	710, 1017, 2279
SmlI	CTYRAG	4 cut(s)	367, 1056, 3271, 3709
SmoI	CTYRAG	4 cut(s)	367, 1056, 3271, 3709
SpeI	ACTAGT	2 cut(s)	2450, 2597
SphI	GCATGC	3 cut(s)	366, 1046, 3587
SsiI	CCGC	7 cut(s)	803, 957, 1262, 1598, 2150, 2491, 3259
SspI	AATATT	2 cut(s)	146, 1384
SspMI	CTAG	9 cut(s)	743, 954, 1079, 1199, 1781, 1937, 2235, 2451, 2598
SstI	GAGCTC	2 cut(s)	705, 3437
StyD4I	CCNGG	7 cut(s)	634, 643, 1246, 2608, 2636, 2637, 3750
StyI	CCWWGG	8 cut(s)	1647, 1941, 1983, 2032, 2574, 2989, 3083, 3130
SwaI	ATTTAAAT	1 cut(s)	2922
TaaI	ACNGT	3 cut(s)	469, 1333, 3066
TaiI	ACGT	1 cut(s)	435
TaqI	TCGA	1 cut(s)	519
TatI	WGTACW	6 cut(s)	1561, 1877, 2506, 2594, 3011, 3454
TscAI	CASTG	6 cut(s)	1648, 2059, 2172, 3105, 3430, 3667
TseFI	GTSAC	8 cut(s)	220, 433, 731, 758, 1356, 1369, 1696, 2777
Tsp45I	GTSAC	8 cut(s)	220, 433, 731, 758, 1356, 1369, 1696, 2777
TspGWI	ACGGA	7 cut(s)	98, 198, 208, 308, 330, 3188, 3297
TspMI	CCCGGG	1 cut(s)	2637
TspRI	CASTG	6 cut(s)	1648, 2059, 2172, 3105, 3430, 3667
Van91I	CCANNNNNTGG	2 cut(s)	1349, 3429
VpaK11BI	GGWCC	8 cut(s)	1244, 1528, 1987, 2693, 2978, 3475, 3647, 3818
XagI	CCTNNNNNAGG	1 cut(s)	2973
XapI	RAATTY	8 cut(s)	249, 539, 569, 592, 831, 1422, 2460, 3720
XceI	RCATGY	9 cut(s)	366, 1046, 1568, 1608, 2134, 2150, 2513, 3021, 3587
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1233, 2856
XmaI	CCCGGG	1 cut(s)	2637
XmiI	GTMKAC	1 cut(s)	2137
XmnI	GAANNNNTTC	2 cut(s)	9, 2094
XspI	CTAG	9 cut(s)	743, 954, 1079, 1199, 1781, 1937, 2235, 2451, 2598
ZrmI	AGTACT	1 cut(s)	1879
Zsp2I	ATGCAT	1 cut(s)	2423

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.