Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0002900

Physical Location & Seq

Genomic Coordinates Reverse (-)

42627324 .. 42643679

Gene Structure 16356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G021400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3762 bp

ATGACGTCGTTTCTGGCTCTACTCGCCGCTCTTCCCTTCTTACAGCTGCTCTTCTTTGGACCTTCGTTGAGTGCAGAGAAGACTGTCTCGCCTGGTCTTCTCACTTCTGACGTCACTTCTCGGAAAACACACTCGGAAGACTACTGTGCAATGTATGATATATGTGGAGAACGCAGTGACGGCAAAGTCTTGAATTGCCCTTATGGTTCCCCCTCTGTGAAGCCTGACGAGCTATTTTCTGCCAAAATCCAAAGTTTGTGTCCAACTATAAGTGGAAATGTTTGTTGCACTGAGCTTCAGTTTGAAACATTGCGAGCACAGGTTCAACAAGCAATTCCATTTCTTGTAGGCTGTCCAGCATGCTTGAGGAATTTCCTAAATTTGTTTTGTGAGCTATCTTGCTCTCCAAAGCAGAGTCTGTTTATCAATGTGACTTCTATCTCCGAGGTTAGTGGCAATATGACTGTGGATGGGATCGACTATTATATATCTGACACTTTTGGAAAAGGACTGTACAACTCGTGTAAGGATGTTAAATTTGGTACTATGAACACACGGGCCATAGAATTTATTGGTGCTGGTGCTAAAAATTTTAAAGAATGGTTTGATTTTCTTGGTGAGAAAGCAGCCCTAGGTTTCCCTGGATCACCTTATGCCATTGACTTCAAGTTAACCGTTCCCGAGTCATCTAATATGGAGCTCATGAACATGTCAGTTTATTCATGTGCTGACACTTCACTAGGCTGCTCTTGTGGTGATTGCCCTTCATCTCCTGCGTGTTCTAATTCTGAACCTCCTCCACACAAGAAAGAGCAATGCTCCATAAAAATTCTATCTATTGAGATCAAATGCATTGATGTTTCAGTAGCCATCATGTATATTCTTTTAGTTTTTGCATTCTTTGTTTGGGGCTTGTTTTATCGGAAAAGAGAAAGGAGGAGAGCTGGATCTACTAAGGAACCATTGTTGAGTGCCATTGATGATGATGATATCAACCCTGTTAACTTACAAAAGGATGAAAGTTTTGCCAGAAAGGGGCATGAGGTGGATCTTCAAGTTACAGAAGAGGTCGAACTATCTTTTATTCAGGAATATATGTCAAGCTTTTTCAGGAGATATGGAATCTGGGTTTCTAGAAATCCCACTCTTGTGCTCTTTTCCTCGGTGGGGATTGTTCTTATACTCTGTATAGGTCTAGTTCGTTTCCAGGTGGAGACACGGCCAGAGAAGCTGTGGGTAGGTCCTGGCAGCAAGGCAGCGGAGGAGAAACAATTCTTTGACAGACATCTTGCTCCCTTCTACAGAATTGAGCAGCTCATTATAGCAACTGTCCCAGATTCAAAGCATGGCGAGTCACCCAGTATTGTCACAGATGACAATATTCAATTACTTTTTGACATACAAGACAAGGTTGACGGAATTCGTGCTAATTATTCTGGCTCAGCGGTAGCTTTAACTGAAATTTGCTTGAAGCCTATTGGCCAAGATTGTGCCACTCAAAGCATTTTGCAGTATTTTAAAATGGACCCTGAAAATTTTGACATCTATGGAGGAGTTGTACATGCCGAGTATTGCTTTCAGCATTATTCTTCTGCAGAAACATGTTTAAGTGCCTTTCAGGCTCCACTTGATCCAAGCACTGCCTTGGGAGGGTTCTCTGGAAACAATTATTCTGAGGCTTCTGCATTCGTAGTCACCTACCCAGTTAATAATGCAGTTGATGATGTAGGAAATGAGAATGGGAAGGCACTGGCTTGGGAGAAGGCTTTTATTCAGTTAGCAAAGGATGAACTGCTGCCAATGGTGCTGTCTCGGAATCTGACTCTCTCATTTTCGGCCGAAAGTTCAATTGAGGAAGAGTTAAAAAGAGAAAGTACTGCAGATGTTATAACCATAATAGTAAGCTATCTGGTAATGTTTCTGTATATATCTCTGACATTGGGAGATGCACCTCAAATATCTTCTCTTTACCTCTCATCCAAGGTCCTGCTTGGTTTGTCAGGAGTATTGCTTGTAATGCTTTCTGTTCTTGGATCTGTTGGATTTTTCAGTGCTGTGGGAGTTAAATCTACATTAATCATAATGGAAGTCATTCCTTTCCTTGTTTTGGCTGTTGGTGTAGATAACATGTGTATATTGGTACATGCTGTGAAACGGCAATCACCGGAGTTACCTTTAGAAAGACGAATAAGTAATGCACTGGTTGAAGTTGGTCCATCCATAACACTGGCTAGTCTGTCTGAGGTTTTGGCCTTTGCGGTGGGAAGCTTCATTCCTATGCCAGCATGCCGAGTCTTCTCCATGTTTGCTGCTCTTGCTGTTTTGCTGGACTTCTTTCTGCAAGTTACTGCATTTGTTGCATTGATATACTTTGACTTTTTGAGAGCTGAGGATGGCAGGGTTGATTGTTTTCCATGCATAAAAGTCTCGTCATCCTTTGTGGAATCCACTGACGGAATCTATCATGGAAGAACTGGATTGTTGGCTCGCTATATGAAGGAAGTGCATGCACGTATTCTTGATTCATGGGTGGTTAAGATGGTTATCGTTGCTATCTTTGTTGGTTTTACCTGGGCAAGCATTGCATTATGTTCTAGGATTGAAACAGGTTTGGAGCAGCAGATTGTCCTTCCCCGGGACTCTTACCTCCAGGGGTATTTCAATAGTATCTCAGAGTATCTCAGGGTTGGACCACCATTATACTTTGTTGTAAAGGATTACAACTACAGCTCAGAATCAAGACATACAAACCAGTTATGCTCCATTAGCCAATGTGAATCGAACTCTCTTTTGAATGAGATATCAAGAGCTTCTTTAACACCAGAATCAAGCTACATTGCTAAGCCAGCTGCCTCTTGGCTTGATGATTTTCTTGTATGGATATCCCCCGAGGCTTTCGGTTGCTGTCGGAAGTTTTTAAATGCTTCTTATTGTCCACCTGATGACCAGCCACCTTGCTGTTCACCTGATGATGGACCTTGTGGCCTTGGTGGAGTGTGCAAAGATTGTACAACTTGCTTTCGCCACTCAGATTTGATTGATGATCGTCCATCTACAGCACAATTTAGAGAGAAGCTTCCTTGGTTCCTTAATGCACTGCCATCCGCTGATTGCGCAAAAGGAGGCCATGGTGCTTACACTAATAGTGTGGATCTCAATGGTTATGGAAATGGCATTATAAGAGCGTCCGAGTTTCGCACCTATCACACGCCACTTAACAAACAAGGTGATTATGTAAATTCAATGAGAGCAGCACGGGAGTTCAGCTCAAGAATGTCTGATTCCTTAAAGATGGATATCTTTCCATACTCGGTGTTCTACATCTTCTTTGAGCAATATCTGGATATATGGAGAATAGCTTTGATCAACATTGCTATAGCTCTTGGTGCAATCTTCCTTGTCTGTCTGCTTATCACTTCCAGCTTATGGAGTTCAGCAATTCTCATACTTGTCTTGGCAATGATTGTTGTGGACCTAATGGGAGTGATGGCCATTTTGGATATCCAACTAAATGCGGTTTCTGTAGTAAATCTCATAATGTCGATCGGAATTGCTGTCGAGTTCTGTGTTCATATAACACATGCTTACTTGGTGAACCATGGGGACAGAAACCAACGAGCAAAGAAAGCTTTAACTAAAGTTGGACCTTCTGTGTTCAGTGGAATCACACTTACAAAGCTAGTTGGAGTGATTGTCCTTTGCTTCTCAAGATCAGAAATTTTTGTGGTGTGTATCTTCTCTCTCAGCTTCAATTACTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1253

Amino Acids

138.25

Weight (kDa)

5.13

Isoelectric Point (pI)

43.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	47 - 278	9.7e-66	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	423 - 614	1.6e-35	NPC1, middle luminal domain
Patched	PF02460	637 - 789	1.4e-32	Patched SSD
Sterol-sensing	PF12349	659 - 808	8.1e-47	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38350 AT4G38350
fragaria_vesca	FvH4_2g16650 FvH4_2g16650 FvH4_2g16650 FvH4_2g16650
malus_domestica	MD10G1139800.v1.1
prunus_persica	Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1 Prupe.8G178600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280781
rosa_laevigata	RLG00000013029
rosa_multiflora	Rmu_sc0001197.1_g000010
rosa_roxburghii	Rroxscaffold_7G00187670
rosa_rugosa	Rorug06G0134400
rosa_samantha	Rh6AG247100 Rh6BG249400 Rh6CG250500 Rh6DG241900
rosa_wichuraiana	Rw6G021400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1889, 3179
AasI	GACNNNNNNGTC	1 cut(s)	185
AatII	GACGTC	2 cut(s)	8, 114
Acc16I	TGCGCA	1 cut(s)	3115
AccB7I	CCANNNNNTGG	1 cut(s)	3426
AccBSI	CCGCTC	1 cut(s)	29
AciI	CCGC	6 cut(s)	27, 1259, 1445, 2258, 3105, 3515
AclWI	GGATC	7 cut(s)	482, 652, 955, 1056, 1625, 2041, 3159
AcoI	YGGCCR	4 cut(s)	1220, 1480, 1836, 3489
AcuI	CTGAAG	1 cut(s)	281
AcyI	GRCGYC	2 cut(s)	5, 111
AfaI	GTAC	6 cut(s)	515, 544, 1560, 1876, 2142, 3010
AfiI	CCNNNNNNNGG	7 cut(s)	1035, 1167, 1650, 2107, 2635, 2972, 3426
AflIII	ACRYGT	3 cut(s)	708, 1601, 2127
AjnI	CCWGG	6 cut(s)	91, 640, 1206, 1243, 2570, 2649
AleI	CACNNNNGTG	1 cut(s)	1148
Alw21I	GWGCWC	3 cut(s)	319, 702, 1155
Alw26I	GTCTC	4 cut(s)	91, 1208, 1815, 2431
AlwI	GGATC	7 cut(s)	482, 652, 955, 1056, 1625, 2041, 3159
AlwNI	CAGNNNCTG	1 cut(s)	418
Ama87I	CYCGRG	3 cut(s)	680, 2634, 2888
AoxI	GGCC	8 cut(s)	558, 1220, 1480, 1836, 2250, 2983, 3124, 3489
ArsI	GACNNNNNNTTYG	2 cut(s)	244, 276
AseI	ATTAAT	1 cut(s)	2075
Asp700I	GAANNNNTTC	2 cut(s)	1271, 2091
AspA2I	CCTAGG	1 cut(s)	631
AspLEI	GCGC	1 cut(s)	3116
AsuC2I	CCSGG	2 cut(s)	2635, 2636
AsuHPI	GGTGA	9 cut(s)	629, 639, 767, 1347, 1687, 2154, 2955, 3239, 3604
AvaI	CYCGRG	3 cut(s)	680, 2634, 2888
AvaII	GGWCC	9 cut(s)	59, 1241, 1525, 1984, 2213, 2690, 2975, 3472, 3644
AvrII	CCTAGG	1 cut(s)	631
BalI	TGGCCA	2 cut(s)	1482, 3491
BanII	GRGCYC	1 cut(s)	702
BauI	CACGAG	1 cut(s)	520
BbsI	GAAGAC	4 cut(s)	86, 89, 144, 2287
Bbv12I	GWGCWC	3 cut(s)	319, 702, 1155
BbvCI	CCTCAGC	1 cut(s)	2388
BceAI	ACGGC	3 cut(s)	196, 1235, 2171
BciT130I	CCWGG	6 cut(s)	93, 642, 1208, 1245, 2572, 2651
BclI	TGATCA	1 cut(s)	3363
BcnI	CCSGG	2 cut(s)	2635, 2636
BcoDI	GTCTC	4 cut(s)	91, 1208, 1815, 2431
BfaI	CTAG	7 cut(s)	632, 740, 1134, 1196, 2232, 2595, 3680
BfmI	CTRYAG	7 cut(s)	1300, 1593, 1878, 2725, 3054, 3375, 3522
BglI	GCCNNNNNGGC	1 cut(s)	1619
BlnI	CCTAGG	1 cut(s)	631
BlpI	GCTNAGC	2 cut(s)	1441, 2841
BmcAI	AGTACT	1 cut(s)	1876
Bme1390I	CCNGG	8 cut(s)	93, 642, 1208, 1245, 2572, 2635, 2636, 2651
Bme18I	GGWCC	9 cut(s)	59, 1241, 1525, 1984, 2213, 2690, 2975, 3472, 3644
BmeT110I	CYCGRG	3 cut(s)	680, 2634, 2888
BmiI	GGNNCC	5 cut(s)	208, 960, 1527, 1623, 3086
BmrFI	CCNGG	8 cut(s)	93, 642, 1208, 1245, 2572, 2635, 2636, 2651
BmrI	ACTGGG	2 cut(s)	1353, 1697
BmsI	GCATC	1 cut(s)	1936
BmuI	ACTGGG	2 cut(s)	1353, 1697
BpiI	GAAGAC	4 cut(s)	86, 89, 144, 2287
BplI	GAGNNNNNCTC	4 cut(s)	803, 835, 3251, 3283
BpmI	CTGGAG	1 cut(s)	2633
Bpu10I	CCTNAGC	1 cut(s)	2388
Bpu1102I	GCTNAGC	2 cut(s)	1441, 2841
BpuEI	CTTGAG	3 cut(s)	385, 3253, 3691
BpuMI	CCSGG	2 cut(s)	2635, 2636
BsaAI	YACGTR	1 cut(s)	2513
BsaBI	GATNNNNATC	2 cut(s)	2543, 3296
BsaHI	GRCGYC	2 cut(s)	5, 111
BsaWI	WCCGGW	1 cut(s)	2164
BsaXI	ACNNNNNCTCC	4 cut(s)	1542, 1572, 3678, 3708
Bsc4I	CCNNNNNNNGG	7 cut(s)	1035, 1167, 1650, 2107, 2635, 2972, 3426
Bse1I	ACTGG	7 cut(s)	1359, 1703, 1755, 2205, 2232, 2479, 2752
Bse3DI	GCAATG	7 cut(s)	156, 308, 821, 2580, 2835, 3369, 3465
Bse8I	GATNNNNATC	2 cut(s)	2543, 3296
BseBI	CCWGG	6 cut(s)	93, 642, 1208, 1245, 2572, 2651
BseGI	GGATG	9 cut(s)	475, 535, 1021, 1792, 1976, 2216, 2398, 2432, 3101
BseJI	GATNNNNATC	2 cut(s)	2543, 3296
BseLI	CCNNNNNNNGG	7 cut(s)	1035, 1167, 1650, 2107, 2635, 2972, 3426
BseMI	GCAATG	7 cut(s)	156, 308, 821, 2580, 2835, 3369, 3465
BseNI	ACTGG	7 cut(s)	1359, 1703, 1755, 2205, 2232, 2479, 2752
BseRI	GAGGAG	4 cut(s)	786, 952, 1277, 1566
BseX3I	CGGCCG	1 cut(s)	1836
BsgI	GTGCAG	1 cut(s)	93
Bsh1285I	CGRYCG	2 cut(s)	1839, 3546
BshFI	GGCC	8 cut(s)	560, 1222, 1482, 1838, 2252, 2985, 3126, 3491
BsiEI	CGRYCG	2 cut(s)	1839, 3546
BsiHKAI	GWGCWC	3 cut(s)	319, 702, 1155
BsiHKCI	CYCGRG	3 cut(s)	680, 2634, 2888
BsiSI	CCGG	2 cut(s)	2165, 2635
BslFI	GGGAC	3 cut(s)	1316, 2651, 3617
BslI	CCNNNNNNNGG	7 cut(s)	1035, 1167, 1650, 2107, 2635, 2972, 3426
BsmAI	GTCTC	4 cut(s)	91, 1208, 1815, 2431
BsmFI	GGGAC	3 cut(s)	1316, 2651, 3617
BsmI	GAATGC	2 cut(s)	896, 1685
BsnI	GGCC	8 cut(s)	560, 1222, 1482, 1838, 2252, 2985, 3126, 3491
BsoBI	CYCGRG	3 cut(s)	680, 2634, 2888
Bsp1286I	GDGCHC	3 cut(s)	319, 702, 1155
Bsp1407I	TGTACA	3 cut(s)	513, 1558, 3008
Bsp1720I	GCTNAGC	2 cut(s)	1441, 2841
Bsp19I	CCATGG	2 cut(s)	3127, 3598
BspACI	CCGC	6 cut(s)	27, 1259, 1445, 2258, 3105, 3515
BspANI	GGCC	8 cut(s)	560, 1222, 1482, 1838, 2252, 2985, 3126, 3491
BspHI	TCATGA	1 cut(s)	702
BspLI	GGNNCC	5 cut(s)	208, 960, 1527, 1623, 3086
BspMAI	CTGCAG	2 cut(s)	1597, 1882
BspPI	GGATC	7 cut(s)	482, 652, 955, 1056, 1625, 2041, 3159
BspQI	GCTCTTC	2 cut(s)	36, 56
BsrBI	CCGCTC	1 cut(s)	29
BsrDI	GCAATG	7 cut(s)	156, 308, 821, 2580, 2835, 3369, 3465
BsrGI	TGTACA	3 cut(s)	513, 1558, 3008
BsrI	ACTGG	7 cut(s)	1359, 1703, 1755, 2205, 2232, 2479, 2752
BssNI	GRCGYC	2 cut(s)	5, 111
BssSI	CACGAG	1 cut(s)	520
BssT1I	CCWWGG	7 cut(s)	631, 1644, 1980, 2986, 3080, 3127, 3598
Bst2BI	CACGAG	1 cut(s)	520
Bst2UI	CCWGG	6 cut(s)	93, 642, 1208, 1245, 2572, 2651
Bst4CI	ACNGT	6 cut(s)	85, 146, 466, 513, 676, 1330
Bst6I	CTCTTC	4 cut(s)	36, 56, 1059, 1851
BstACI	GRCGYC	2 cut(s)	5, 111
BstAPI	GCANNNNNTGC	2 cut(s)	2357, 2582
BstAUI	TGTACA	3 cut(s)	513, 1558, 3008
BstBAI	YACGTR	1 cut(s)	2513
BstC8I	GCNNGC	8 cut(s)	315, 361, 2283, 2287, 2488, 2508, 2578, 2847
BstDSI	CCRYGG	2 cut(s)	3127, 3598
BstF5I	GGATG	9 cut(s)	475, 535, 1021, 1792, 1976, 2216, 2398, 2432, 3101
BstHHI	GCGC	1 cut(s)	3116
BstMAI	GTCTC	4 cut(s)	91, 1208, 1815, 2431
BstMCI	CGRYCG	2 cut(s)	1839, 3546
BstNI	CCWGG	6 cut(s)	93, 642, 1208, 1245, 2572, 2651
BstNSI	RCATGY	9 cut(s)	363, 712, 1565, 1605, 2131, 2147, 2289, 2510, 3584
BstSCI	CCNGG	8 cut(s)	91, 640, 1206, 1243, 2570, 2633, 2634, 2649
BstSFI	CTRYAG	7 cut(s)	1300, 1593, 1878, 2725, 3054, 3375, 3522
BstV2I	GAAGAC	4 cut(s)	86, 89, 144, 2287
BstX2I	RGATCY	4 cut(s)	947, 1048, 2033, 3151
BstXI	CCANNNNNNTGG	1 cut(s)	2227
BstYI	RGATCY	4 cut(s)	947, 1048, 2033, 3151
BstZI	CGGCCG	1 cut(s)	1836
BsuRI	GGCC	8 cut(s)	560, 1222, 1482, 1838, 2252, 2985, 3126, 3491
BtgI	CCRYGG	2 cut(s)	3127, 3598
BtsCI	GGATG	9 cut(s)	475, 535, 1021, 1792, 1976, 2216, 2398, 2432, 3101
BtsI	GCAGTG	3 cut(s)	181, 1638, 3095
Cac8I	GCNNGC	8 cut(s)	315, 361, 2283, 2287, 2488, 2508, 2578, 2847
CaiI	CAGNNNCTG	1 cut(s)	418
CciI	TCATGA	1 cut(s)	702
CfoI	GCGC	1 cut(s)	3116
Cfr9I	CCCGGG	1 cut(s)	2634
CseI	GACGC	1 cut(s)	3174
Csp6I	GTAC	6 cut(s)	514, 543, 1559, 1875, 2141, 3009
CviQI	GTAC	6 cut(s)	514, 543, 1559, 1875, 2141, 3009
DraI	TTTAAA	3 cut(s)	595, 1519, 2919
DrdI	GACNNNNNNGTC	1 cut(s)	185
DseDI	GACNNNNNNGTC	1 cut(s)	185
EaeI	YGGCCR	4 cut(s)	1220, 1480, 1836, 3489
EagI	CGGCCG	1 cut(s)	1836
Eam1104I	CTCTTC	4 cut(s)	36, 56, 1059, 1851
EarI	CTCTTC	4 cut(s)	36, 56, 1059, 1851
Ecl136II	GAGCTC	1 cut(s)	700
EclXI	CGGCCG	1 cut(s)	1836
Eco130I	CCWWGG	7 cut(s)	631, 1644, 1980, 2986, 3080, 3127, 3598
Eco24I	GRGCYC	1 cut(s)	702
Eco32I	GATATC	5 cut(s)	991, 2802, 2883, 3298, 3502
Eco47I	GGWCC	9 cut(s)	59, 1241, 1525, 1984, 2213, 2690, 2975, 3472, 3644
Eco52I	CGGCCG	1 cut(s)	1836
Eco53kI	GAGCTC	1 cut(s)	700
Eco57I	CTGAAG	1 cut(s)	281
Eco88I	CYCGRG	3 cut(s)	680, 2634, 2888
EcoICRI	GAGCTC	1 cut(s)	700
EcoO109I	RGGNCCY	2 cut(s)	1241, 1984
EcoRI	GAATTC	1 cut(s)	1419
EcoRII	CCWGG	6 cut(s)	91, 640, 1206, 1243, 2570, 2649
EcoRV	GATATC	5 cut(s)	991, 2802, 2883, 3298, 3502
EcoT14I	CCWWGG	7 cut(s)	631, 1644, 1980, 2986, 3080, 3127, 3598
EcoT22I	ATGCAT	2 cut(s)	854, 2420
EcoT38I	GRGCYC	1 cut(s)	702
ErhI	CCWWGG	7 cut(s)	631, 1644, 1980, 2986, 3080, 3127, 3598
FalI	AAGNNNNNCTT	2 cut(s)	2797, 2829
FaqI	GGGAC	3 cut(s)	1316, 2651, 3617
FbaI	TGATCA	1 cut(s)	3363
FokI	GGATG	9 cut(s)	482, 542, 1028, 1799, 1963, 2203, 2405, 2419, 3088
FriOI	GRGCYC	1 cut(s)	702
FspBI	CTAG	7 cut(s)	632, 740, 1134, 1196, 2232, 2595, 3680
FspI	TGCGCA	1 cut(s)	3115
GlaI	GCGC	1 cut(s)	3115
GsuI	CTGGAG	1 cut(s)	2633
HaeIII	GGCC	8 cut(s)	560, 1222, 1482, 1838, 2252, 2985, 3126, 3491
HapII	CCGG	2 cut(s)	2165, 2635
HgaI	GACGC	1 cut(s)	3174
HhaI	GCGC	1 cut(s)	3116
Hin1I	GRCGYC	2 cut(s)	5, 111
Hin6I	GCGC	1 cut(s)	3114
HinP1I	GCGC	1 cut(s)	3114
HincII	GTYRAC	3 cut(s)	672, 1003, 1414
HindII	GTYRAC	3 cut(s)	672, 1003, 1414
HindIII	AAGCTT	4 cut(s)	1102, 2266, 3074, 3627
HpaI	GTTAAC	2 cut(s)	672, 1003
HpaII	CCGG	2 cut(s)	2165, 2635
HphI	GGTGA	9 cut(s)	629, 639, 767, 1347, 1687, 2154, 2955, 3239, 3604
Hpy166II	GTNNAC	7 cut(s)	672, 1003, 1414, 2936, 2963, 3472, 3595
Hpy8I	GTNNAC	7 cut(s)	672, 1003, 1414, 2936, 2963, 3472, 3595
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	9 cut(s)	46, 72, 774, 1306, 1738, 1756, 2491, 2639, 3657
HpyCH4III	ACNGT	6 cut(s)	85, 146, 466, 513, 676, 1330
HpyCH4IV	ACGT	3 cut(s)	5, 111, 2512
HpySE526I	ACGT	3 cut(s)	5, 111, 2512
Hsp92I	GRCGYC	2 cut(s)	5, 111
HspAI	GCGC	1 cut(s)	3114
Ksp22I	TGATCA	1 cut(s)	3363
KspAI	GTTAAC	2 cut(s)	672, 1003
LguI	GCTCTTC	2 cut(s)	36, 56
LmnI	GCTCC	6 cut(s)	697, 824, 1297, 1627, 2614, 2765
LweI	GCATC	1 cut(s)	1936
MaeI	CTAG	7 cut(s)	632, 740, 1134, 1196, 2232, 2595, 3680
MaeII	ACGT	3 cut(s)	5, 111, 2512
MaeIII	GTNAC	9 cut(s)	112, 176, 430, 1057, 1353, 1366, 1693, 2169, 2344
MbiI	CCGCTC	1 cut(s)	29
MfeI	CAATTG	1 cut(s)	1848
MflI	RGATCY	4 cut(s)	947, 1048, 2033, 3151
MhlI	GDGCHC	3 cut(s)	319, 702, 1155
MlsI	TGGCCA	2 cut(s)	1482, 3491
MluNI	TGGCCA	2 cut(s)	1482, 3491
MlyI	GAGTC	6 cut(s)	424, 692, 1361, 1816, 2301, 2633
MmeI	TCCRAC	7 cut(s)	287, 2020, 2668, 2888, 3529, 3622, 3664
Mox20I	TGGCCA	2 cut(s)	1482, 3491
Mph1103I	ATGCAT	2 cut(s)	854, 2420
MroXI	GAANNNNTTC	2 cut(s)	1271, 2091
MscI	TGGCCA	2 cut(s)	1482, 3491
MslI	CAYNNNNRTG	3 cut(s)	707, 1148, 2276
Msp20I	TGGCCA	2 cut(s)	1482, 3491
MspA1I	CMGCKG	5 cut(s)	46, 1259, 1445, 2849, 3107
MspI	CCGG	2 cut(s)	2165, 2635
MspR9I	CCNGG	8 cut(s)	93, 642, 1208, 1245, 2572, 2635, 2636, 2651
MunI	CAATTG	1 cut(s)	1848
Mva1269I	GAATGC	2 cut(s)	896, 1685
MvaI	CCWGG	6 cut(s)	93, 642, 1208, 1245, 2572, 2651
NciI	CCSGG	2 cut(s)	2635, 2636
NcoI	CCATGG	2 cut(s)	3127, 3598
NlaIV	GGNNCC	5 cut(s)	208, 960, 1527, 1623, 3086
NmeAIII	GCCGAG	2 cut(s)	1591, 2315
NmuCI	GTSAC	6 cut(s)	112, 176, 430, 1353, 1366, 1693
NsbI	TGCGCA	1 cut(s)	3115
NsiI	ATGCAT	2 cut(s)	854, 2420
NspI	RCATGY	9 cut(s)	363, 712, 1565, 1605, 2131, 2147, 2289, 2510, 3584
OliI	CACNNNNGTG	1 cut(s)	1148
PaeI	GCATGC	3 cut(s)	363, 2289, 2510
PagI	TCATGA	1 cut(s)	702
PciI	ACATGT	3 cut(s)	708, 1601, 2127
PciSI	GCTCTTC	2 cut(s)	36, 56
PctI	GAATGC	2 cut(s)	896, 1685
PdmI	GAANNNNTTC	2 cut(s)	1271, 2091
PflMI	CCANNNNNTGG	1 cut(s)	3426
Ple19I	CGATCG	1 cut(s)	3546
PleI	GAGTC	6 cut(s)	423, 691, 1360, 1816, 2300, 2633
PpsI	GAGTC	6 cut(s)	423, 691, 1360, 1816, 2300, 2633
Ppu21I	YACGTR	1 cut(s)	2513
PpuMI	RGGWCCY	2 cut(s)	1241, 1984
PscI	ACATGT	3 cut(s)	708, 1601, 2127
PshBI	ATTAAT	1 cut(s)	2075
PsiI	TTATAA	2 cut(s)	1889, 3179
Psp124BI	GAGCTC	1 cut(s)	702
Psp5II	RGGWCCY	2 cut(s)	1241, 1984
Psp6I	CCWGG	6 cut(s)	91, 640, 1206, 1243, 2570, 2649
PspGI	CCWGG	6 cut(s)	91, 640, 1206, 1243, 2570, 2649
PspN4I	GGNNCC	5 cut(s)	208, 960, 1527, 1623, 3086
PspPPI	RGGWCCY	2 cut(s)	1241, 1984
PstI	CTGCAG	2 cut(s)	1597, 1882
PstNI	CAGNNNCTG	1 cut(s)	418
PsuI	RGATCY	4 cut(s)	947, 1048, 2033, 3151
PvuI	CGATCG	1 cut(s)	3546
PvuII	CAGCTG	2 cut(s)	46, 2849
RsaI	GTAC	6 cut(s)	515, 544, 1560, 1876, 2142, 3010
RsaNI	GTAC	6 cut(s)	514, 543, 1559, 1875, 2141, 3009
RseI	CAYNNNNRTG	3 cut(s)	707, 1148, 2276
SacI	GAGCTC	1 cut(s)	702
SapI	GCTCTTC	2 cut(s)	36, 56
ScaI	AGTACT	1 cut(s)	1876
SchI	GAGTC	6 cut(s)	424, 692, 1361, 1816, 2301, 2633
ScrFI	CCNGG	8 cut(s)	93, 642, 1208, 1245, 2572, 2635, 2636, 2651
SduI	GDGCHC	3 cut(s)	319, 702, 1155
SfaNI	GCATC	1 cut(s)	1936
SfcI	CTRYAG	7 cut(s)	1300, 1593, 1878, 2725, 3054, 3375, 3522
SinI	GGWCC	9 cut(s)	59, 1241, 1525, 1984, 2213, 2690, 2975, 3472, 3644
SmaI	CCCGGG	1 cut(s)	2636
SmiMI	CAYNNNNRTG	3 cut(s)	707, 1148, 2276
SmlI	CTYRAG	3 cut(s)	364, 3268, 3706
SmoI	CTYRAG	3 cut(s)	364, 3268, 3706
SphI	GCATGC	3 cut(s)	363, 2289, 2510
SsiI	CCGC	6 cut(s)	27, 1259, 1445, 2258, 3105, 3515
SspI	AATATT	1 cut(s)	1381
SspMI	CTAG	7 cut(s)	632, 740, 1134, 1196, 2232, 2595, 3680
SstI	GAGCTC	1 cut(s)	702
StyD4I	CCNGG	8 cut(s)	91, 640, 1206, 1243, 2570, 2633, 2634, 2649
StyI	CCWWGG	7 cut(s)	631, 1644, 1980, 2986, 3080, 3127, 3598
TaaI	ACNGT	6 cut(s)	85, 146, 466, 513, 676, 1330
TaiI	ACGT	3 cut(s)	8, 114, 2515
TaqI	TCGA	5 cut(s)	477, 1071, 2780, 3542, 3558
TatI	WGTACW	4 cut(s)	513, 1558, 1874, 3008
TauI	GCSGC	1 cut(s)	29
TseFI	GTSAC	6 cut(s)	112, 176, 430, 1353, 1366, 1693
Tsp45I	GTSAC	6 cut(s)	112, 176, 430, 1353, 1366, 1693
TspGWI	ACGGA	2 cut(s)	1431, 2469
TspMI	CCCGGG	1 cut(s)	2634
Van91I	CCANNNNNTGG	1 cut(s)	3426
VpaK11BI	GGWCC	9 cut(s)	59, 1241, 1525, 1984, 2213, 2690, 2975, 3472, 3644
VspI	ATTAAT	1 cut(s)	2075
XbaI	TCTAGA	1 cut(s)	1133
XceI	RCATGY	9 cut(s)	363, 712, 1565, 1605, 2131, 2147, 2289, 2510, 3584
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1230, 2853
XmaI	CCCGGG	1 cut(s)	2634
XmaJI	CCTAGG	1 cut(s)	631
XmnI	GAANNNNTTC	2 cut(s)	1271, 2091
XspI	CTAG	7 cut(s)	632, 740, 1134, 1196, 2232, 2595, 3680
ZraI	GACGTC	2 cut(s)	6, 112
ZrmI	AGTACT	1 cut(s)	1876
Zsp2I	ATGCAT	2 cut(s)	854, 2420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.