AT4G39380

TSL-kinase interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007256
Physical Location & Seq
Reverse (-)
18308854 .. 18312748
3895 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G39380.2

Sequence Viewer

Length: 1557 bp
ATGGCTAGAAAGAAGACTGAAACTGCAACTGAGTCCTCAAAGGAGATCAAAGCGAAAGGAAAATTCACCAGAGCCAAGAAGTCTATGAAAACAACTACTAAGAATATCCATCAATCTGGAAAACATAATAGTGGAAAAGAGGAGAAGTATAAATGTTCAGCTCAGCCTTCATCTTCTCAAGAGCAAAGGCTGATACATACTGAAGACCAAACTCAAAAAGTACCTGTCCTTGATATCCATTCGAGCAAGGACGTTCCACGTTCTGGAAAAATGAAGCTGCAGCTCTTTCCTTTGGATGCTCATACCCGTGAAGGATTGGAAAAGGACGGCTTTCACCCATACTTGGAGCTCACTCTTAGCTCACGGAAGAAGATTTCTTCAGTGCTTCAACGCATTCACAGTAAGTGGGGCAGCTCAGAGATTGCTCGTGGAGATCCCACGTTGTACCCTTATGATACATCGGTACTTGCTTCTGGTCATAAATGGGTAGCAAACAGCAACATCACTACAGGCAATGTCTATGAAGCTATTGGAGCTCCATTTATTTTCCGCTTACGGTATGGGTGGTCCTCTGCGACTGAAAATAAAACCAATGAACCGCCATCTCCTTCAACTCCAGGTACAGGAACGCAGATGTTAGGTTCAGAGAACCCATTGACTATGCTGTCCTCTCCAAATCAAGTGACTGACACACCTCCCTTGGAAAACTTACCGCATGATGGACAAGTTGAGTCAACGGAGAATAAGATAAACGATGGATCTGGCCCGACATTGCTTTTTTGGGATGATGGATTAACAAGCCTAAGTATTGGTGGCTTGCTTTCAGAAGTATCATTGAAGGGAAACTTTGGAAATCATTGTAAAAATTCAAACGCAGGGAATGCAAATGCAACTCTCTGGGAAGACAATCTTACTAACATAAGCATTGGAGGTCTATTTTCTGAGGCTTCTTTACAAGACAGATGGCATATGAACAATGAACGAGAACCAGCTCATAATGGTCAGTCTATTGGAAGTATAGGAGGCTTACTATCTGAAGCATCAATTCTAGGAGAAGGAAGGATTAGTGACTGTAACAAAACATGGGAAACAAGGAGAGCTATCAAACAACCACCATTACCTCTAATCTCTGATTCATTAGATGCTTTCCTCGTGAACCAAACAGATAACCGTCGAGCTCCTTGCCATGCCCCTCTTCCTGAGTCGTCACATTCGTCTATACTCGATGCAGAAGATACGTGCCATGCATTTTCATTTCGAAAGCGTACCACAATAACACCAAAGGTTCACGATCAGGTAAGTGGAGAAGTTGAGAAGGAGAAACAGATTGATGAATCGAACCCCGCAAAACCATTATTGGGTTCCTCGGTGTTTAATCAAGACAGCAGCCTCGGATTATCAGGAATCAAGTGGGCTGAATCACGGGGACCATTTGATTTCGGCTTATCTTCATCGCGAAAGTTTGCTAATGGAGACAGCGTAAGCTTTGGTGCTGTAGTAAAAGATTTGCAGGAAATGGAGCCTTTAGAAAAGAAAATAAAGCTTCAAAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
No domains found.

Protein Analysis

518

Amino Acids

56.77

Weight (kDa)

7.29

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 664
AccB7I CCANNNNNTGG 1 cut(s) 263
AccII CGCG 1 cut(s) 1456
AciI CCGC 4 cut(s) 550, 599, 713, 1344
AclWI GGATC 2 cut(s) 428, 766
AcsI RAATTY 2 cut(s) 62, 865
AcuI CTGAAG 3 cut(s) 222, 363, 1056
AfaI GTAC 5 cut(s) 222, 446, 465, 622, 1267
AfiI CCNNNNNNNGG 5 cut(s) 263, 343, 623, 719, 1358
AgsI TTSAA 5 cut(s) 389, 612, 838, 870, 1547
AjnI CCWGG 1 cut(s) 616
Alw21I GWGCWC 3 cut(s) 351, 538, 1180
Alw26I GTCTC 1 cut(s) 1467
AlwI GGATC 2 cut(s) 428, 766
AoxI GGCC 1 cut(s) 763
ApeKI GCWGC 4 cut(s) 277, 280, 411, 1386
ApoI RAATTY 2 cut(s) 62, 865
AspS9I GGNCC 3 cut(s) 567, 764, 1427
AsuHPI GGTGA 2 cut(s) 58, 326
AsuII TTCGAA 1 cut(s) 1258
AvaII GGWCC 2 cut(s) 567, 1427
BaeI ACNNNNGTAYC 2 cut(s) 447, 480
BanII GRGCYC 3 cut(s) 351, 538, 1180
BauI CACGAG 2 cut(s) 426, 1151
BbsI GAAGAC 3 cut(s) 20, 210, 909
Bbv12I GWGCWC 3 cut(s) 351, 538, 1180
BbvI GCAGC 4 cut(s) 264, 292, 423, 1398
BccI CCATC 6 cut(s) 117, 610, 713, 749, 782, 957
BceAI ACGGC 1 cut(s) 343
BcgI CGANNNNNNTGC 2 cut(s) 1164, 1198
BciT130I CCWGG 1 cut(s) 618
BcoDI GTCTC 1 cut(s) 1467
BfaI CTAG 2 cut(s) 6, 1049
BfmI CTRYAG 3 cut(s) 278, 507, 1494
BisI GCNGC 4 cut(s) 278, 281, 412, 1387
BlpI GCTNAGC 1 cut(s) 162
BlsI GCNGC 4 cut(s) 279, 282, 413, 1388
Bme1390I CCNGG 1 cut(s) 618
Bme18I GGWCC 2 cut(s) 567, 1427
BmgT120I GGNCC 3 cut(s) 567, 764, 1427
BmiI GGNNCC 3 cut(s) 1363, 1428, 1521
BmrFI CCNGG 1 cut(s) 618
BmsI GCATC 4 cut(s) 286, 1049, 1132, 1216
BpiI GAAGAC 3 cut(s) 20, 210, 909
BpmI CTGGAG 1 cut(s) 600
Bpu1102I GCTNAGC 1 cut(s) 162
Bpu14I TTCGAA 1 cut(s) 1258
BpuEI CTTGAG 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 1239
BsaJI CCNNGG 3 cut(s) 699, 1365, 1390
BsaXI ACNNNNNCTCC 2 cut(s) 1464, 1494
Bsc4I CCNNNNNNNGG 5 cut(s) 263, 343, 623, 719, 1358
Bse3DI GCAATG 2 cut(s) 520, 770
BseBI CCWGG 1 cut(s) 618
BseDI CCNNGG 3 cut(s) 699, 1365, 1390
BseGI GGATG 2 cut(s) 301, 790
BseLI CCNNNNNNNGG 5 cut(s) 263, 343, 623, 719, 1358
BseMI GCAATG 2 cut(s) 520, 770
BseMII CTCAG 5 cut(s) 21, 176, 429, 933, 1191
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 4 cut(s) 264, 292, 423, 1398
Bsh1236I CGCG 1 cut(s) 1456
BshFI GGCC 1 cut(s) 765
BsiHKAI GWGCWC 3 cut(s) 351, 538, 1180
BslFI GGGAC 1 cut(s) 1440
BslI CCNNNNNNNGG 5 cut(s) 263, 343, 623, 719, 1358
BsmAI GTCTC 1 cut(s) 1467
BsmFI GGGAC 1 cut(s) 1440
BsmI GAATGC 2 cut(s) 393, 886
BsnI GGCC 1 cut(s) 765
Bsp119I TTCGAA 1 cut(s) 1258
Bsp1286I GDGCHC 3 cut(s) 351, 538, 1180
Bsp143I GATC 4 cut(s) 45, 433, 758, 1291
Bsp1720I GCTNAGC 1 cut(s) 162
Bsp68I TCGCGA 1 cut(s) 1456
BspACI CCGC 4 cut(s) 550, 599, 713, 1344
BspANI GGCC 1 cut(s) 765
BspCNI CTCAG 5 cut(s) 22, 175, 428, 934, 1192
BspFNI CGCG 1 cut(s) 1456
BspLI GGNNCC 3 cut(s) 1363, 1428, 1521
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 2 cut(s) 428, 766
BspT104I TTCGAA 1 cut(s) 1258
BsrDI GCAATG 2 cut(s) 520, 770
BssECI CCNNGG 3 cut(s) 699, 1365, 1390
BssMI GATC 4 cut(s) 45, 433, 758, 1291
BssSI CACGAG 2 cut(s) 426, 1151
BssT1I CCWWGG 1 cut(s) 699
Bst2BI CACGAG 2 cut(s) 426, 1151
Bst2UI CCWGG 1 cut(s) 618
Bst4CI ACNGT 4 cut(s) 401, 558, 1073, 1172
Bst6I CTCTTC 1 cut(s) 1200
BstAPI GCANNNNNTGC 1 cut(s) 881
BstBAI YACGTR 1 cut(s) 1239
BstBI TTCGAA 1 cut(s) 1258
BstC8I GCNNGC 1 cut(s) 818
BstDEI CTNAG 8 cut(s) 30, 99, 162, 356, 415, 803, 942, 1200
BstF5I GGATG 2 cut(s) 301, 790
BstFNI CGCG 1 cut(s) 1456
BstKTI GATC 4 cut(s) 48, 436, 761, 1294
BstMAI GTCTC 1 cut(s) 1467
BstMBI GATC 4 cut(s) 45, 433, 758, 1291
BstMWI GCNNNNNNNGC 3 cut(s) 533, 881, 1549
BstNI CCWGG 1 cut(s) 618
BstSCI CCNGG 1 cut(s) 616
BstSFI CTRYAG 3 cut(s) 278, 507, 1494
BstUI CGCG 1 cut(s) 1456
BstV1I GCAGC 4 cut(s) 264, 292, 423, 1398
BstV2I GAAGAC 3 cut(s) 20, 210, 909
BstX2I RGATCY 2 cut(s) 433, 758
BstXI CCANNNNNNTGG 1 cut(s) 116
BstYI RGATCY 2 cut(s) 433, 758
BsuRI GGCC 1 cut(s) 765
BtgZI GCGATG 1 cut(s) 1437
BtsCI GGATG 2 cut(s) 301, 790
BtsIMutI CAGTG 1 cut(s) 387
BtuMI TCGCGA 1 cut(s) 1456
Cac8I GCNNGC 1 cut(s) 818
Cfr13I GGNCC 3 cut(s) 567, 764, 1427
Csp6I GTAC 5 cut(s) 221, 445, 464, 621, 1266
CviAII CATG 4 cut(s) 716, 1083, 1187, 1244
CviQI GTAC 5 cut(s) 221, 445, 464, 621, 1266
DdeI CTNAG 8 cut(s) 30, 99, 162, 356, 415, 803, 942, 1200
DpnI GATC 4 cut(s) 47, 435, 760, 1293
DpnII GATC 4 cut(s) 45, 433, 758, 1291
DrdI GACNNNNNNGTC 1 cut(s) 664
DseDI GACNNNNNNGTC 1 cut(s) 664
Eam1104I CTCTTC 1 cut(s) 1200
EarI CTCTTC 1 cut(s) 1200
Ecl136II GAGCTC 3 cut(s) 349, 536, 1178
Eco130I CCWWGG 1 cut(s) 699
Eco24I GRGCYC 3 cut(s) 351, 538, 1180
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 2 cut(s) 567, 1427
Eco53kI GAGCTC 3 cut(s) 349, 536, 1178
Eco57I CTGAAG 3 cut(s) 222, 363, 1056
EcoICRI GAGCTC 3 cut(s) 349, 536, 1178
EcoRII CCWGG 1 cut(s) 616
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 699
EcoT22I ATGCAT 1 cut(s) 1249
EcoT38I GRGCYC 3 cut(s) 351, 538, 1180
ErhI CCWWGG 1 cut(s) 699
FaeI CATG 4 cut(s) 719, 1086, 1190, 1247
FalI AAGNNNNNCTT 6 cut(s) 314, 346, 830, 862, 894, 926
FaqI GGGAC 1 cut(s) 1440
FatI CATG 4 cut(s) 715, 1082, 1186, 1243
FauI CCCGC 1 cut(s) 1351
FauNDI CATATG 1 cut(s) 969
Fnu4HI GCNGC 4 cut(s) 278, 281, 412, 1387
FokI GGATG 2 cut(s) 308, 797
FriOI GRGCYC 3 cut(s) 351, 538, 1180
Fsp4HI GCNGC 4 cut(s) 278, 281, 412, 1387
FspBI CTAG 2 cut(s) 6, 1049
GluI GCNGC 4 cut(s) 278, 281, 412, 1387
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 1 cut(s) 765
Hin1II CATG 4 cut(s) 719, 1086, 1190, 1247
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HindIII AAGCTT 2 cut(s) 1483, 1541
HinfI GANTC 7 cut(s) 32, 731, 1133, 1202, 1334, 1404, 1418
HphI GGTGA 2 cut(s) 58, 326
Hpy166II GTNNAC 3 cut(s) 735, 1156, 1288
Hpy188I TCNGA 7 cut(s) 418, 646, 826, 943, 1036, 1132, 1394
Hpy188III TCNNGA 9 cut(s) 117, 179, 264, 1153, 1199, 1289, 1379, 1401, 1455
Hpy8I GTNNAC 3 cut(s) 735, 1156, 1288
Hpy99I CGWCG 1 cut(s) 1176
HpyAV CCTTC 7 cut(s) 177, 305, 618, 832, 1049, 1053, 1309
HpyCH4III ACNGT 4 cut(s) 401, 558, 1073, 1172
HpyCH4IV ACGT 4 cut(s) 252, 259, 440, 1238
HpyCH4V TGCA 7 cut(s) 26, 280, 884, 890, 1229, 1247, 1510
HpyF10VI GCNNNNNNNGC 3 cut(s) 533, 881, 1549
HpyF3I CTNAG 8 cut(s) 30, 99, 162, 356, 415, 803, 942, 1200
HpySE526I ACGT 4 cut(s) 252, 259, 440, 1238
Hsp92II CATG 4 cut(s) 719, 1086, 1190, 1247
Kzo9I GATC 4 cut(s) 45, 433, 758, 1291
LmnI GCTCC 5 cut(s) 346, 533, 541, 1183, 1519
Lsp1109I GCAGC 4 cut(s) 264, 292, 423, 1398
LweI GCATC 4 cut(s) 286, 1049, 1132, 1216
MaeI CTAG 2 cut(s) 6, 1049
MaeII ACGT 4 cut(s) 252, 259, 440, 1238
MaeIII GTNAC 4 cut(s) 682, 1067, 1073, 1206
MalI GATC 4 cut(s) 47, 435, 760, 1293
MboI GATC 4 cut(s) 45, 433, 758, 1291
MflI RGATCY 2 cut(s) 433, 758
MhlI GDGCHC 3 cut(s) 351, 538, 1180
MluCI AATT 3 cut(s) 62, 865, 1044
MlyI GAGTC 3 cut(s) 41, 740, 1211
Mph1103I ATGCAT 1 cut(s) 1249
MseI TTAA 2 cut(s) 794, 1374
MslI CAYNNNNRTG 2 cut(s) 129, 306
MspR9I CCNGG 1 cut(s) 618
Mva1269I GAATGC 2 cut(s) 393, 886
MvaI CCWGG 1 cut(s) 618
MvnI CGCG 1 cut(s) 1456
MwoI GCNNNNNNNGC 3 cut(s) 533, 881, 1549
NdeI CATATG 1 cut(s) 969
NdeII GATC 4 cut(s) 45, 433, 758, 1291
NlaIII CATG 4 cut(s) 719, 1086, 1190, 1247
NlaIV GGNNCC 3 cut(s) 1363, 1428, 1521
NmuCI GTSAC 3 cut(s) 682, 1067, 1206
NruI TCGCGA 1 cut(s) 1456
NsiI ATGCAT 1 cut(s) 1249
NspV TTCGAA 1 cut(s) 1258
PcsI WCGNNNNNNNCGW 1 cut(s) 1211
PctI GAATGC 2 cut(s) 393, 886
PfeI GAWTC 4 cut(s) 1133, 1334, 1404, 1418
PflMI CCANNNNNTGG 1 cut(s) 263
PkrI GCNGC 4 cut(s) 279, 282, 413, 1388
PleI GAGTC 3 cut(s) 40, 739, 1210
PpsI GAGTC 3 cut(s) 40, 739, 1210
Ppu21I YACGTR 1 cut(s) 1239
Psp124BI GAGCTC 3 cut(s) 351, 538, 1180
Psp6I CCWGG 1 cut(s) 616
PspGI CCWGG 1 cut(s) 616
PspN4I GGNNCC 3 cut(s) 1363, 1428, 1521
PspPI GGNCC 3 cut(s) 567, 764, 1427
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 2 cut(s) 433, 758
RruI TCGCGA 1 cut(s) 1456
RsaI GTAC 5 cut(s) 222, 446, 465, 622, 1267
RsaNI GTAC 5 cut(s) 221, 445, 464, 621, 1266
RseI CAYNNNNRTG 2 cut(s) 129, 306
SacI GAGCTC 3 cut(s) 351, 538, 1180
SaqAI TTAA 2 cut(s) 794, 1374
SatI GCNGC 4 cut(s) 278, 281, 412, 1387
Sau3AI GATC 4 cut(s) 45, 433, 758, 1291
Sau96I GGNCC 3 cut(s) 567, 764, 1427
SchI GAGTC 3 cut(s) 41, 740, 1211
ScrFI CCNGG 1 cut(s) 618
SduI GDGCHC 3 cut(s) 351, 538, 1180
SfaNI GCATC 4 cut(s) 286, 1049, 1132, 1216
SfcI CTRYAG 3 cut(s) 278, 507, 1494
SfuI TTCGAA 1 cut(s) 1258
SinI GGWCC 2 cut(s) 567, 1427
SmiMI CAYNNNNRTG 2 cut(s) 129, 306
SmlI CTYRAG 1 cut(s) 177
SmoI CTYRAG 1 cut(s) 177
Sse9I AATT 3 cut(s) 62, 865, 1044
SsiI CCGC 4 cut(s) 550, 599, 713, 1344
SspMI CTAG 2 cut(s) 6, 1049
SstI GAGCTC 3 cut(s) 351, 538, 1180
StyD4I CCNGG 1 cut(s) 616
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 4 cut(s) 401, 558, 1073, 1172
TaiI ACGT 4 cut(s) 255, 262, 443, 1241
TaqI TCGA 5 cut(s) 242, 1174, 1224, 1258, 1337
TasI AATT 3 cut(s) 62, 865, 1044
TfiI GAWTC 4 cut(s) 1133, 1334, 1404, 1418
Tru1I TTAA 2 cut(s) 794, 1374
Tru9I TTAA 2 cut(s) 794, 1374
TscAI CASTG 1 cut(s) 387
TseFI GTSAC 3 cut(s) 682, 1067, 1206
TseI GCWGC 4 cut(s) 277, 280, 411, 1386
Tsp45I GTSAC 3 cut(s) 682, 1067, 1206
TspGWI ACGGA 2 cut(s) 379, 752
TspRI CASTG 1 cut(s) 387
Van91I CCANNNNNTGG 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 567, 1427
XapI RAATTY 2 cut(s) 62, 865
XspI CTAG 2 cut(s) 6, 1049
Zsp2I ATGCAT 1 cut(s) 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.