Rh2AG270900

TSL-kinase interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007256
Physical Location & Seq
Forward (+)
32265766 .. 32269030
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG270900.1

Sequence Viewer

Length: 1452 bp
ATGAAAGCTCCTAGACAGAGAAACTCAAAAGTGATCAAGGTTCCTATAAAGGGCAGAGCAAGTAAAGGTGGCATGGGGGAACAAAAATCTACAAGTAGAACTACACAGCACTGTCATAGAGAACCAGGGAAAGGTGACGACTTCTTCAATGAAAAAGATGAGCATTTATCACCTTGCAATGCAGCTAAAGGCTTGGATTTGGAGATTTCTGATGATGTACAGTGTTCAGAACCAACAAAGAAGCTGCCTTTTTATGGCATTAATGGAGGGAAACCACTTCTTCCTCATTCAAAGATCAAATTGCAGCTTTTTCCTGTAAATGAAGGCATTCGAATAGGATTGGAAAAGGATGGATTTCATCCATATTTGGAACTCACTCTTAGAGCACGGAAGAAGATTTCTTCTGTTCTAAAGCACCTTATTAGCAAATGGGGCAGTTCAAGTGCTGCTGTTGGTGAGCCGGTTCTATTCCCATACAGGATACAGGACAGTGTATCTAGTAACAGAAGATGGACGTTGAATGATGCTGAAACCAGTGCAGGAGATGTCTATGCAGCTATTGAAAGCCCTGAAATTTTTCGCTTAAGGTATGGTTGGTTCTCTAATGAACCTAAAACCTCCGAGAAGCCTTCTACATCAGCTTATGGCAAGGTTGACTTACAATCTGAAAGCATACAGAGAGCTACTAGCACCCCTGCAGAGAATGCATATGAAGTAGAGAACCACACTGTGTTAACAAGCAAATATATGGAATCAATAAATGAAAGGACAAATTCAAATGCTGAGAAGATTTCTAATGTAGATGCTGATCTTTTGAGTGATGGCCAGAGGGGGGTGGATGGTAAACATGCACTGGTTGTTGATACTGTAACTGACATAAGCATTGGAGGCCTCCTTTCTGAAGCATCCTTACAGGCTAAGTGCAATAATGGAAATAAAAAACAGAGAGAGAAGGCTAAAATCCCGCATCCTGTGGGCTTAAAGAGTGATTCAAAATCATTTGAGTTGTGGGCTGAATGTCCATCTATAAGCATTGGTGGCATCCTGTCTGAAGTATCCTTACAGGGAAAAATCAATCGTTTTGGTGCGCAATCAACTATGAGCAATCCAAGCTTGCAGCCAGCTCATTCAATTTATGATAATCCTCAAGGTTCAAGGCCATCTGCTCATGATTCTTGCTTATCAATTTTGGATGCTGAAGAAACCTGTCATGCATTTCCCTCTCAAAAGTTTTCTCTGGGAAAAGATGTTCTGGGTATCGGAGGAAGTTGTGGAGGTTCGAGCCAGAACTCTGGTTTCAAACTGTTCAAGTTTCCCCTTCCAGCCAAGGGTAATAGATTAGCTGAGCTTCCAAAAGACCATGCTCGGCAAGAATCTAATACAGAGCGGATGAATTGTTCATATTTGAATAATGATGAACGGAGCCTTGGGCTATCAGGCATCAAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
No domains found.

Protein Analysis

483

Amino Acids

52.83

Weight (kDa)

8.66

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1089
AccBSI CCGCTC 1 cut(s) 1387
AciI CCGC 2 cut(s) 965, 1387
AcoI YGGCCR 1 cut(s) 823
AcsI RAATTY 2 cut(s) 573, 772
AcuI CTGAAG 3 cut(s) 921, 1071, 1218
AdeI CACNNNGTG 1 cut(s) 730
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 5 cut(s) 50, 131, 254, 832, 1328
AflII CTTAAG 1 cut(s) 583
AjnI CCWGG 1 cut(s) 124
AjuI GAANNNNNNNTTGG 2 cut(s) 1410, 1442
AloI GAACNNNNNNTCC 4 cut(s) 1233, 1265, 1381, 1413
Alw21I GWGCWC 1 cut(s) 388
AoxI GGCC 3 cut(s) 823, 889, 1157
ApeKI GCWGC 6 cut(s) 182, 244, 304, 446, 554, 1117
ApoI RAATTY 2 cut(s) 573, 772
AseI ATTAAT 1 cut(s) 261
Asp700I GAANNNNTTC 2 cut(s) 327, 576
AspLEI GCGC 1 cut(s) 1090
AsuHPI GGTGA 3 cut(s) 146, 162, 467
AsuII TTCGAA 1 cut(s) 331
BalI TGGCCA 1 cut(s) 825
BarI GAAGNNNNNNTAC 4 cut(s) 894, 926, 1044, 1076
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1129
BccI CCATC 6 cut(s) 344, 504, 815, 833, 1030, 1168
BciT130I CCWGG 1 cut(s) 126
BciVI GTATCC 2 cut(s) 474, 1066
BclI TGATCA 1 cut(s) 33
BfaI CTAG 3 cut(s) 12, 498, 687
BfmI CTRYAG 1 cut(s) 696
BfrI CTTAAG 1 cut(s) 583
BfuI GTATCC 2 cut(s) 474, 1066
BisI GCNGC 6 cut(s) 183, 245, 305, 447, 555, 1118
BlpI GCTNAGC 1 cut(s) 1344
BlsI GCNGC 6 cut(s) 184, 246, 306, 448, 556, 1119
Bme1390I CCNGG 1 cut(s) 126
BmiI GGNNCC 2 cut(s) 42, 1424
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 6 cut(s) 514, 793, 914, 976, 1050, 1183
Bpu1102I GCTNAGC 1 cut(s) 1344
Bpu14I TTCGAA 1 cut(s) 331
BpuEI CTTGAG 1 cut(s) 1131
BsaBI GATNNNNATC 1 cut(s) 807
BsaJI CCNNGG 3 cut(s) 125, 1326, 1426
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 131, 254, 832, 1328
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 534, 858
Bse3DI GCAATG 1 cut(s) 184
Bse8I GATNNNNATC 1 cut(s) 807
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 3 cut(s) 125, 1326, 1426
BseGI GGATG 8 cut(s) 355, 358, 844, 905, 967, 1041, 1198, 1395
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 5 cut(s) 50, 131, 254, 832, 1328
BseMI GCAATG 1 cut(s) 184
BseMII CTCAG 2 cut(s) 774, 1335
BseNI ACTGG 2 cut(s) 534, 858
BseXI GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1129
BsgI GTGCAG 1 cut(s) 558
BshFI GGCC 3 cut(s) 825, 891, 1159
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 1 cut(s) 461
BslI CCNNNNNNNGG 5 cut(s) 50, 131, 254, 832, 1328
BsmI GAATGC 2 cut(s) 327, 709
BsnI GGCC 3 cut(s) 825, 891, 1159
Bsp119I TTCGAA 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 388
Bsp1407I TGTACA 1 cut(s) 217
Bsp143I GATC 3 cut(s) 33, 294, 808
Bsp1720I GCTNAGC 1 cut(s) 1344
BspACI CCGC 2 cut(s) 965, 1387
BspANI GGCC 3 cut(s) 825, 891, 1159
BspCNI CTCAG 2 cut(s) 775, 1336
BspHI TCATGA 1 cut(s) 1168
BspLI GGNNCC 2 cut(s) 42, 1424
BspMAI CTGCAG 1 cut(s) 700
BspT104I TTCGAA 1 cut(s) 331
BspTI CTTAAG 1 cut(s) 583
BsrBI CCGCTC 1 cut(s) 1387
BsrDI GCAATG 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 460
BsrGI TGTACA 1 cut(s) 217
BsrI ACTGG 2 cut(s) 534, 858
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 3 cut(s) 125, 1326, 1426
BssMI GATC 3 cut(s) 33, 294, 808
BssT1I CCWWGG 2 cut(s) 1326, 1426
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 6 cut(s) 113, 222, 491, 730, 868, 1305
BstAFI CTTAAG 1 cut(s) 583
BstAPI GCANNNNNTGC 1 cut(s) 704
BstAUI TGTACA 1 cut(s) 217
BstBI TTCGAA 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 1115, 1122
BstDEI CTNAG 4 cut(s) 380, 783, 918, 1344
BstF5I GGATG 8 cut(s) 355, 358, 844, 905, 967, 1041, 1198, 1395
BstHHI GCGC 1 cut(s) 1090
BstKTI GATC 3 cut(s) 36, 297, 811
BstMBI GATC 3 cut(s) 33, 294, 808
BstMWI GCNNNNNNNGC 5 cut(s) 432, 704, 888, 1038, 1110
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 1 cut(s) 851
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 1 cut(s) 696
BstV1I GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1129
BstXI CCANNNNNNTGG 1 cut(s) 1292
BsuI GTATCC 2 cut(s) 474, 1066
BsuRI GGCC 3 cut(s) 825, 891, 1159
BtsCI GGATG 8 cut(s) 355, 358, 844, 905, 967, 1041, 1198, 1395
BtsIMutI CAGTG 6 cut(s) 109, 227, 496, 541, 726, 851
Cac8I GCNNGC 2 cut(s) 1115, 1122
CciI TCATGA 1 cut(s) 1168
CfoI GCGC 1 cut(s) 1090
Cfr10I RCCGGY 1 cut(s) 460
Csp6I GTAC 1 cut(s) 218
CviAII CATG 5 cut(s) 73, 848, 1169, 1211, 1361
CviQI GTAC 1 cut(s) 218
DdeI CTNAG 4 cut(s) 380, 783, 918, 1344
DpnI GATC 3 cut(s) 35, 296, 810
DpnII GATC 3 cut(s) 33, 294, 808
DraIII CACNNNGTG 1 cut(s) 730
EaeI YGGCCR 1 cut(s) 823
Eco130I CCWWGG 2 cut(s) 1326, 1426
Eco147I AGGCCT 1 cut(s) 891
Eco57I CTGAAG 3 cut(s) 921, 1071, 1218
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 2 cut(s) 1326, 1426
EcoT22I ATGCAT 2 cut(s) 709, 1216
ErhI CCWWGG 2 cut(s) 1326, 1426
FaeI CATG 5 cut(s) 76, 851, 1172, 1214, 1364
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FatI CATG 5 cut(s) 72, 847, 1168, 1210, 1360
FauI CCCGC 1 cut(s) 972
FauNDI CATATG 1 cut(s) 709
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 6 cut(s) 183, 245, 305, 447, 555, 1118
FokI GGATG 8 cut(s) 345, 362, 851, 892, 954, 1028, 1205, 1402
Fsp4HI GCNGC 6 cut(s) 183, 245, 305, 447, 555, 1118
FspBI CTAG 3 cut(s) 12, 498, 687
FspI TGCGCA 1 cut(s) 1089
GlaI GCGC 1 cut(s) 1089
GluI GCNGC 6 cut(s) 183, 245, 305, 447, 555, 1118
HaeIII GGCC 3 cut(s) 825, 891, 1159
HapII CCGG 1 cut(s) 461
HhaI GCGC 1 cut(s) 1090
Hin1II CATG 5 cut(s) 76, 851, 1172, 1214, 1364
Hin6I GCGC 1 cut(s) 1088
HinP1I GCGC 1 cut(s) 1088
HincII GTYRAC 2 cut(s) 655, 735
HindII GTYRAC 2 cut(s) 655, 735
HindIII AAGCTT 1 cut(s) 1111
HinfI GANTC 4 cut(s) 752, 989, 1172, 1373
HpaI GTTAAC 1 cut(s) 735
HpaII CCGG 1 cut(s) 461
HphI GGTGA 3 cut(s) 146, 162, 467
Hpy166II GTNNAC 3 cut(s) 655, 735, 845
Hpy188I TCNGA 7 cut(s) 211, 229, 622, 667, 901, 1051, 1262
Hpy188III TCNNGA 1 cut(s) 1169
Hpy8I GTNNAC 3 cut(s) 655, 735, 845
HpyAV CCTTC 4 cut(s) 317, 639, 946, 1328
HpyCH4III ACNGT 6 cut(s) 113, 222, 491, 730, 868, 1305
HpyCH4IV ACGT 1 cut(s) 515
HpyF10VI GCNNNNNNNGC 5 cut(s) 432, 704, 888, 1038, 1110
HpyF3I CTNAG 4 cut(s) 380, 783, 918, 1344
HpySE526I ACGT 1 cut(s) 515
Hsp92II CATG 5 cut(s) 76, 851, 1172, 1214, 1364
HspAI GCGC 1 cut(s) 1088
Ksp22I TGATCA 1 cut(s) 33
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 3 cut(s) 33, 294, 808
LmnI GCTCC 2 cut(s) 13, 1422
Lsp1109I GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1129
LweI GCATC 6 cut(s) 514, 793, 914, 976, 1050, 1183
MaeI CTAG 3 cut(s) 12, 498, 687
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 3 cut(s) 134, 500, 868
MalI GATC 3 cut(s) 35, 296, 810
MbiI CCGCTC 1 cut(s) 1387
MboI GATC 3 cut(s) 33, 294, 808
MboII GAAGA 8 cut(s) 136, 272, 393, 403, 406, 519, 799, 1211
MhlI GDGCHC 1 cut(s) 388
MlsI TGGCCA 1 cut(s) 825
MluCI AATT 6 cut(s) 299, 573, 772, 1131, 1185, 1393
MluNI TGGCCA 1 cut(s) 825
Mox20I TGGCCA 1 cut(s) 825
Mph1103I ATGCAT 2 cut(s) 709, 1216
MroXI GAANNNNTTC 2 cut(s) 327, 576
MscI TGGCCA 1 cut(s) 825
MseI TTAA 4 cut(s) 261, 584, 734, 980
Msp20I TGGCCA 1 cut(s) 825
MspCI CTTAAG 1 cut(s) 583
MspI CCGG 1 cut(s) 461
MspR9I CCNGG 1 cut(s) 126
Mva1269I GAATGC 2 cut(s) 327, 709
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 5 cut(s) 432, 704, 888, 1038, 1110
NdeI CATATG 1 cut(s) 709
NdeII GATC 3 cut(s) 33, 294, 808
NlaIII CATG 5 cut(s) 76, 851, 1172, 1214, 1364
NlaIV GGNNCC 2 cut(s) 42, 1424
NmeAIII GCCGAG 1 cut(s) 1345
NmuCI GTSAC 1 cut(s) 134
NsbI TGCGCA 1 cut(s) 1089
NsiI ATGCAT 2 cut(s) 709, 1216
NspI RCATGY 1 cut(s) 851
NspV TTCGAA 1 cut(s) 331
PagI TCATGA 1 cut(s) 1168
PceI AGGCCT 1 cut(s) 891
PctI GAATGC 2 cut(s) 327, 709
PdmI GAANNNNTTC 2 cut(s) 327, 576
PfeI GAWTC 4 cut(s) 752, 989, 1172, 1373
PkrI GCNGC 6 cut(s) 184, 246, 306, 448, 556, 1119
PshBI ATTAAT 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 42, 1424
PsrI GAACNNNNNNTAC 2 cut(s) 581, 613
PstI CTGCAG 1 cut(s) 700
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
SaqAI TTAA 4 cut(s) 261, 584, 734, 980
SatI GCNGC 6 cut(s) 183, 245, 305, 447, 555, 1118
Sau3AI GATC 3 cut(s) 33, 294, 808
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 6 cut(s) 514, 793, 914, 976, 1050, 1183
SfcI CTRYAG 1 cut(s) 696
SfuI TTCGAA 1 cut(s) 331
SmlI CTYRAG 2 cut(s) 583, 1146
SmoI CTYRAG 2 cut(s) 583, 1146
Sse9I AATT 6 cut(s) 299, 573, 772, 1131, 1185, 1393
SseBI AGGCCT 1 cut(s) 891
SsiI CCGC 2 cut(s) 965, 1387
SspMI CTAG 3 cut(s) 12, 498, 687
StuI AGGCCT 1 cut(s) 891
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 2 cut(s) 1326, 1426
TaaI ACNGT 6 cut(s) 113, 222, 491, 730, 868, 1305
TaiI ACGT 1 cut(s) 518
TaqI TCGA 2 cut(s) 331, 1280
TasI AATT 6 cut(s) 299, 573, 772, 1131, 1185, 1393
TatI WGTACW 1 cut(s) 217
TfiI GAWTC 4 cut(s) 752, 989, 1172, 1373
Tru1I TTAA 4 cut(s) 261, 584, 734, 980
Tru9I TTAA 4 cut(s) 261, 584, 734, 980
TscAI CASTG 6 cut(s) 116, 227, 496, 541, 733, 858
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 6 cut(s) 182, 244, 304, 446, 554, 1117
Tsp45I GTSAC 1 cut(s) 134
TspGWI ACGGA 2 cut(s) 403, 1435
TspRI CASTG 6 cut(s) 116, 227, 496, 541, 733, 858
Vha464I CTTAAG 1 cut(s) 583
VspI ATTAAT 1 cut(s) 261
XapI RAATTY 2 cut(s) 573, 772
XceI RCATGY 1 cut(s) 851
XmnI GAANNNNTTC 2 cut(s) 327, 576
XspI CTAG 3 cut(s) 12, 498, 687
Zsp2I ATGCAT 2 cut(s) 709, 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.