Rh2BG282500

TSL-kinase interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007256
Physical Location & Seq
Forward (+)
31040983 .. 31045602
4620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG282500.1

Sequence Viewer

Length: 1464 bp
ATGAAAGCTCCTAGACAGAGAAACTCAAAAGTGATCAAGGTTCCTATAAAGGGCAGAGCAAGTAAAGGTGGCATGGGGGAACAAAAATCTACAAGTAGAACTACACAGCACTGTCATAGAGAACCGGGGAAAGGTGACGACTTCTTCAATGAAAAAGATGAGCATTTATCACCTTGCAATGCAGCTAAAGGCTTGGATTTGGAGATTCCTGATGATGTACAGTGTTCAGAACCAACAAAGAAGCTGCCTTTTTATGGAATTAATGGAGGGAAACCACTTCTTCCTCATTCAAAGATCAAATTGCAGCTTTTTCCTGTAAATGAAGGCATTCGAATAGGATTGGAAAAGGATGGATTTCATCCATATTTGGAACTCACTCTTAGAGCACGGAAGAAGATTTCTTCTGTTCTAAAGCACCTTATTAGCAAATGGGGCAGTTCAAGTGCTGCTGTTGGTGAGCTGGTTCTATTCCCATACAGGATACAGGACAGTGTATCTTGTAACAGAAGATGGACGTTGAATGATGCTGAAACCAGTGCAGGAGATGTCTATGCAGCTATTGAATGCCCTGAAATTTTTCGCTTAAGGTATGGTTGGTTCTCTAATGAACCTAAAACCTCCGAGAAGCCTTCTACATCAGCTTATGGCAAGGTTGACTTACAATCTGAAAGCATACAGAGAGCTACTAGCACCCCTGCAGAGAATGCATATGAAGTAGAGAACCACACTGTGTTAACAAGCAAATATATGGAATCAATAAATGAAAGGACAAATTCAAATGCTGAGAAGATTTCTAATGTAGATGCTGATCTTTTGAGTGATGGCCAGAGGGGGGTGGATGGTAAACATGCACTGGTTGTTGATACTGTAACTGACATAAGCATTGGAGGCCTCCTTTCTGAAGCATCCTTACAGGCTAAGTGCAATAATGGAAATAAAAAACAGAGAGAGAAGGCTAAAACCCCGCATCCTGTGGGCATAAAGAGTGATTCAAAATCATTTGAGTTGTGGGCTGATTGTCCATCTATAAGCATTGGCGGCATCCTGTCTGAAGTATCCTTACAGGGAAAAATCAATCGTTTTGGTGCGCAATCAACTATGAGCAATCCAAGCTTGCAGCCAGCTCATTCAATTTATGATAATCCTCAAGGTTCAAGGCCATCTGCTCATGATTCTTGCTTATCAATTTTGGATGCTGAAGAAACCTGTCATGCATTTCCCTCTCAAAAGTTTTCTCTGGGAAAAGATGTTCTGGGTATCGGAGGAAGTTGTGGAGGTTCGAGCCAGAACTCTGGTTTCAAACTGTTCAAGTTTCCTCTTCCAGCCAAGGTTTGTGTAATTCTGTTTGCTTATTTGTTTCTTTTACTGCGACTGTGCATAAATGTATGTTGCTTCATTCTACTAGGGTTTGAAATCCATTACCTTTCAGTCTACTTTACAGTAATCTTGCCACATAAAACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
No domains found.

Protein Analysis

487

Amino Acids

53.42

Weight (kDa)

8.61

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1089
AccI GTMKAC 1 cut(s) 1431
AciI CCGC 2 cut(s) 965, 1038
AcoI YGGCCR 1 cut(s) 823
AcsI RAATTY 2 cut(s) 573, 772
AcuI CTGAAG 3 cut(s) 921, 1071, 1218
AdeI CACNNNGTG 1 cut(s) 730
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 4 cut(s) 50, 131, 254, 832
AflII CTTAAG 1 cut(s) 583
AloI GAACNNNNNNTCC 2 cut(s) 1233, 1265
Alw21I GWGCWC 1 cut(s) 388
AoxI GGCC 3 cut(s) 823, 889, 1157
ApeKI GCWGC 6 cut(s) 182, 244, 304, 446, 554, 1117
ApoI RAATTY 2 cut(s) 573, 772
AseI ATTAAT 1 cut(s) 261
Asp700I GAANNNNTTC 2 cut(s) 327, 576
AspLEI GCGC 1 cut(s) 1090
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 3 cut(s) 146, 162, 467
AsuII TTCGAA 1 cut(s) 331
BalI TGGCCA 1 cut(s) 825
BarI GAAGNNNNNNTAC 4 cut(s) 894, 926, 1044, 1076
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1129
BccI CCATC 6 cut(s) 344, 504, 815, 833, 1030, 1168
BciVI GTATCC 2 cut(s) 474, 1066
BclI TGATCA 1 cut(s) 33
BcnI CCSGG 1 cut(s) 126
BfaI CTAG 3 cut(s) 12, 687, 1403
BfmI CTRYAG 1 cut(s) 696
BfrI CTTAAG 1 cut(s) 583
BfuI GTATCC 2 cut(s) 474, 1066
BisI GCNGC 7 cut(s) 183, 245, 305, 447, 555, 1039, 1118
BlsI GCNGC 7 cut(s) 184, 246, 306, 448, 556, 1040, 1119
Bme1390I CCNGG 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 42
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 6 cut(s) 514, 793, 914, 976, 1050, 1183
Bpu14I TTCGAA 1 cut(s) 331
BpuEI CTTGAG 1 cut(s) 1131
BpuMI CCSGG 1 cut(s) 126
BsaBI GATNNNNATC 1 cut(s) 807
BsaJI CCNNGG 2 cut(s) 125, 1326
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 131, 254, 832
Bse1I ACTGG 2 cut(s) 534, 858
Bse3DI GCAATG 1 cut(s) 184
Bse8I GATNNNNATC 1 cut(s) 807
BseDI CCNNGG 2 cut(s) 125, 1326
BseGI GGATG 7 cut(s) 355, 358, 844, 905, 967, 1041, 1198
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 4 cut(s) 50, 131, 254, 832
BseMI GCAATG 1 cut(s) 184
BseMII CTCAG 1 cut(s) 774
BseNI ACTGG 2 cut(s) 534, 858
BseXI GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1129
BsgI GTGCAG 1 cut(s) 558
BshFI GGCC 3 cut(s) 825, 891, 1159
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 1 cut(s) 125
BslI CCNNNNNNNGG 4 cut(s) 50, 131, 254, 832
BsmI GAATGC 3 cut(s) 327, 569, 709
BsnI GGCC 3 cut(s) 825, 891, 1159
Bsp119I TTCGAA 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 388
Bsp1407I TGTACA 1 cut(s) 217
Bsp143I GATC 3 cut(s) 33, 294, 808
BspACI CCGC 2 cut(s) 965, 1038
BspANI GGCC 3 cut(s) 825, 891, 1159
BspCNI CTCAG 1 cut(s) 775
BspHI TCATGA 1 cut(s) 1168
BspLI GGNNCC 1 cut(s) 42
BspMAI CTGCAG 1 cut(s) 700
BspT104I TTCGAA 1 cut(s) 331
BspTI CTTAAG 1 cut(s) 583
BsrDI GCAATG 1 cut(s) 184
BsrGI TGTACA 1 cut(s) 217
BsrI ACTGG 2 cut(s) 534, 858
BssECI CCNNGG 2 cut(s) 125, 1326
BssMI GATC 3 cut(s) 33, 294, 808
BssT1I CCWWGG 1 cut(s) 1326
Bst4CI ACNGT 8 cut(s) 113, 222, 491, 730, 868, 1305, 1374, 1441
Bst6I CTCTTC 1 cut(s) 1323
BstAFI CTTAAG 1 cut(s) 583
BstAPI GCANNNNNTGC 1 cut(s) 704
BstAUI TGTACA 1 cut(s) 217
BstBI TTCGAA 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 1115, 1122
BstDEI CTNAG 3 cut(s) 380, 783, 918
BstF5I GGATG 7 cut(s) 355, 358, 844, 905, 967, 1041, 1198
BstHHI GCGC 1 cut(s) 1090
BstKTI GATC 3 cut(s) 36, 297, 811
BstMBI GATC 3 cut(s) 33, 294, 808
BstMWI GCNNNNNNNGC 5 cut(s) 432, 704, 888, 1038, 1110
BstNSI RCATGY 1 cut(s) 851
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 1 cut(s) 696
BstV1I GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1129
BstXI CCANNNNNNTGG 1 cut(s) 1292
BsuI GTATCC 2 cut(s) 474, 1066
BsuRI GGCC 3 cut(s) 825, 891, 1159
BtsCI GGATG 7 cut(s) 355, 358, 844, 905, 967, 1041, 1198
BtsIMutI CAGTG 6 cut(s) 109, 227, 496, 541, 726, 851
Cac8I GCNNGC 2 cut(s) 1115, 1122
CciI TCATGA 1 cut(s) 1168
CfoI GCGC 1 cut(s) 1090
Csp6I GTAC 1 cut(s) 218
CviAII CATG 4 cut(s) 73, 848, 1169, 1211
CviQI GTAC 1 cut(s) 218
DdeI CTNAG 3 cut(s) 380, 783, 918
DpnI GATC 3 cut(s) 35, 296, 810
DpnII GATC 3 cut(s) 33, 294, 808
DraIII CACNNNGTG 1 cut(s) 730
EaeI YGGCCR 1 cut(s) 823
Eam1104I CTCTTC 1 cut(s) 1323
EarI CTCTTC 1 cut(s) 1323
Eco130I CCWWGG 1 cut(s) 1326
Eco147I AGGCCT 1 cut(s) 891
Eco57I CTGAAG 3 cut(s) 921, 1071, 1218
EcoT14I CCWWGG 1 cut(s) 1326
EcoT22I ATGCAT 2 cut(s) 709, 1216
ErhI CCWWGG 1 cut(s) 1326
FaeI CATG 4 cut(s) 76, 851, 1172, 1214
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FatI CATG 4 cut(s) 72, 847, 1168, 1210
FauI CCCGC 1 cut(s) 972
FauNDI CATATG 1 cut(s) 709
FbaI TGATCA 1 cut(s) 33
FblI GTMKAC 1 cut(s) 1431
Fnu4HI GCNGC 7 cut(s) 183, 245, 305, 447, 555, 1039, 1118
FokI GGATG 7 cut(s) 345, 362, 851, 892, 954, 1028, 1205
Fsp4HI GCNGC 7 cut(s) 183, 245, 305, 447, 555, 1039, 1118
FspBI CTAG 3 cut(s) 12, 687, 1403
FspI TGCGCA 1 cut(s) 1089
GlaI GCGC 1 cut(s) 1089
GluI GCNGC 7 cut(s) 183, 245, 305, 447, 555, 1039, 1118
HaeIII GGCC 3 cut(s) 825, 891, 1159
HapII CCGG 1 cut(s) 125
HhaI GCGC 1 cut(s) 1090
Hin1II CATG 4 cut(s) 76, 851, 1172, 1214
Hin6I GCGC 1 cut(s) 1088
HinP1I GCGC 1 cut(s) 1088
HincII GTYRAC 2 cut(s) 655, 735
HindII GTYRAC 2 cut(s) 655, 735
HindIII AAGCTT 1 cut(s) 1111
HinfI GANTC 4 cut(s) 205, 752, 989, 1172
HpaI GTTAAC 1 cut(s) 735
HpaII CCGG 1 cut(s) 125
HphI GGTGA 3 cut(s) 146, 162, 467
Hpy166II GTNNAC 4 cut(s) 655, 735, 845, 1432
Hpy188I TCNGA 6 cut(s) 229, 622, 667, 901, 1051, 1262
Hpy188III TCNNGA 2 cut(s) 209, 1169
Hpy8I GTNNAC 4 cut(s) 655, 735, 845, 1432
HpyAV CCTTC 3 cut(s) 317, 639, 946
HpyCH4III ACNGT 8 cut(s) 113, 222, 491, 730, 868, 1305, 1374, 1441
HpyCH4IV ACGT 2 cut(s) 515, 1460
HpyF10VI GCNNNNNNNGC 5 cut(s) 432, 704, 888, 1038, 1110
HpyF3I CTNAG 3 cut(s) 380, 783, 918
HpySE526I ACGT 2 cut(s) 515, 1460
Hsp92II CATG 4 cut(s) 76, 851, 1172, 1214
HspAI GCGC 1 cut(s) 1088
Ksp22I TGATCA 1 cut(s) 33
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 3 cut(s) 33, 294, 808
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1129
LweI GCATC 6 cut(s) 514, 793, 914, 976, 1050, 1183
MaeI CTAG 3 cut(s) 12, 687, 1403
MaeII ACGT 2 cut(s) 515, 1460
MaeIII GTNAC 3 cut(s) 134, 500, 868
MalI GATC 3 cut(s) 35, 296, 810
MboI GATC 3 cut(s) 33, 294, 808
MboII GAAGA 9 cut(s) 136, 272, 393, 403, 406, 519, 799, 1211, 1310
MhlI GDGCHC 1 cut(s) 388
MlsI TGGCCA 1 cut(s) 825
MluCI AATT 7 cut(s) 258, 299, 573, 772, 1131, 1185, 1338
MluNI TGGCCA 1 cut(s) 825
Mox20I TGGCCA 1 cut(s) 825
Mph1103I ATGCAT 2 cut(s) 709, 1216
MroXI GAANNNNTTC 2 cut(s) 327, 576
MscI TGGCCA 1 cut(s) 825
MseI TTAA 3 cut(s) 261, 584, 734
Msp20I TGGCCA 1 cut(s) 825
MspCI CTTAAG 1 cut(s) 583
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 1 cut(s) 126
Mva1269I GAATGC 3 cut(s) 327, 569, 709
MwoI GCNNNNNNNGC 5 cut(s) 432, 704, 888, 1038, 1110
NciI CCSGG 1 cut(s) 126
NdeI CATATG 1 cut(s) 709
NdeII GATC 3 cut(s) 33, 294, 808
NlaIII CATG 4 cut(s) 76, 851, 1172, 1214
NlaIV GGNNCC 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 134
NsbI TGCGCA 1 cut(s) 1089
NsiI ATGCAT 2 cut(s) 709, 1216
NspI RCATGY 1 cut(s) 851
NspV TTCGAA 1 cut(s) 331
PagI TCATGA 1 cut(s) 1168
PceI AGGCCT 1 cut(s) 891
PctI GAATGC 3 cut(s) 327, 569, 709
PdmI GAANNNNTTC 2 cut(s) 327, 576
PfeI GAWTC 4 cut(s) 205, 752, 989, 1172
PkrI GCNGC 7 cut(s) 184, 246, 306, 448, 556, 1040, 1119
PshBI ATTAAT 1 cut(s) 261
PspN4I GGNNCC 1 cut(s) 42
PsrI GAACNNNNNNTAC 2 cut(s) 581, 613
PstI CTGCAG 1 cut(s) 700
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
SaqAI TTAA 3 cut(s) 261, 584, 734
SatI GCNGC 7 cut(s) 183, 245, 305, 447, 555, 1039, 1118
Sau3AI GATC 3 cut(s) 33, 294, 808
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 6 cut(s) 514, 793, 914, 976, 1050, 1183
SfcI CTRYAG 1 cut(s) 696
SfuI TTCGAA 1 cut(s) 331
SmlI CTYRAG 2 cut(s) 583, 1146
SmoI CTYRAG 2 cut(s) 583, 1146
Sse9I AATT 7 cut(s) 258, 299, 573, 772, 1131, 1185, 1338
SseBI AGGCCT 1 cut(s) 891
SsiI CCGC 2 cut(s) 965, 1038
SspMI CTAG 3 cut(s) 12, 687, 1403
StuI AGGCCT 1 cut(s) 891
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 1326
TaaI ACNGT 8 cut(s) 113, 222, 491, 730, 868, 1305, 1374, 1441
TaiI ACGT 2 cut(s) 518, 1463
TaqI TCGA 2 cut(s) 331, 1280
TasI AATT 7 cut(s) 258, 299, 573, 772, 1131, 1185, 1338
TatI WGTACW 1 cut(s) 217
TauI GCSGC 1 cut(s) 1041
TfiI GAWTC 4 cut(s) 205, 752, 989, 1172
Tru1I TTAA 3 cut(s) 261, 584, 734
Tru9I TTAA 3 cut(s) 261, 584, 734
TscAI CASTG 6 cut(s) 116, 227, 496, 541, 733, 858
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 6 cut(s) 182, 244, 304, 446, 554, 1117
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 8 cut(s) 17, 165, 336, 347, 621, 726, 777, 1384
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 6 cut(s) 116, 227, 496, 541, 733, 858
Vha464I CTTAAG 1 cut(s) 583
VspI ATTAAT 1 cut(s) 261
XapI RAATTY 2 cut(s) 573, 772
XceI RCATGY 1 cut(s) 851
XmiI GTMKAC 1 cut(s) 1431
XmnI GAANNNNTTC 2 cut(s) 327, 576
XspI CTAG 3 cut(s) 12, 687, 1403
Zsp2I ATGCAT 2 cut(s) 709, 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.