RLG00000018381

TSL-kinase interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007256
Physical Location & Seq
Forward (+)
29007988 .. 29011238
3251 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018381

Sequence Viewer

Length: 1518 bp
ATGAAAGCTCCTAGACAGAGAAACTCAAAAGTGATCAAGGTTCCTATAAAGGGCAGAGCAAGTAAAGGTGGCATGGGGGAACAGACATCTACAAGTAGAACTACACAGCACTGTCATAGAGAACCAGGAAAAGGTGACGACTTCTTCAATGAAAAAGTTGAGCATTTATCACCTTGCAATGCAGCTAAAGGCTTGGATTTGGAGATTTCTGATGATGTACAGTGTTCAAAACCAACAAAGAAGCTGCCTTTTTATGGCATTAATGGAGGGAAACCACTTCTTCCTCATTCAAAGATCAAATTGCAGCTTTTTCCTGTAAATGAAGGCATCCGAATAGGATTGGAAAAGGATGGATTTCATCCATATTTGGAACTCACTCTTAGAGCACGGAAGAAGATTTCTTCTGTTCTAAAGCACCTTATTAGCAAATGGGGCAGTTCAAGCGCTGCTGTTGGTGAGCCAGTTCTATTCCCATACAGGATACAGGACAGTGTATCTAATAACAGAAGATGGATGTTGAATGATGCTGAAACCAGTGCAGGAGATGTCTATGCAGCTATTGAAAGCCCTGAAATTTTTCGCTTAAGGTATGGTTGGTTCTCTAATGAACCTAAAACCTCCGAGAAGCCTTCTACATCAGCTTATGACAAGGTTGACTTACAATCTGAAAGCATACAGAGAGCTACTAGCACCCCTGCAGAGAATGCATATGAAGTCGAGAACCACTCTGTGTTAACAAGCAAATATATGGAATCAATAAATGAAAGGACAAATTCAAATGTAGATGCTGATCTTTTGAGTGATGGCCAGAGGCGGGTGGATGGTAAACATGCACTGGTTGTTGATACTGTAACTGACATTGGAGGCCTCCTTTCTGAAGCATCCTTACAGGCTAAGTGCAATAATAGAAATAAAAAACGGAGAGAGAAGGCTAAAACCCCACATCCTGTGGGCATAAAGAGTGATTCAAAATCATTTGAGTTGTGGGCTGATTGTCCATCTATAAGCATTGGTGGCATCCTGTCTGAAGTATCCTTACAGGGAAAAATCAATCGTTTTGGTGCGCAATCAACTATGAGCAATCCAGGCATGCAGCCAGCTCATTCAATTTCTGATAATCCGCAAGGTTCAAGGCCATCTGCTCATGATTCTTGCTTATCAATTTTGGATGCTGAAGAAACCTGTCATGCATTTCCCTCTCAAAAGTTTTCTCTGGGAAAAGATGTTCTGGGTATCGGAGGAAGTTGTGGAGGTTCGAGCCAGAGCTCTGGTTTCAAACTCTTCAAGTTTCCTCTTCCAGCCAAGGGTAATAGATTAGTTGAGCTTCCAAAAGAGCATGCTTGGCAAGAATCTAATACAGAACGGATGAATTGTTCATATTTGAACAATGATGAACGGAGCCTTGGGCTATCAGGCATCAAATGGACCGACTCTTTGGGGCCCTTTGATCTTGGCCTGCCTGTCTCCCAGAAGCTCTGTAATGCAGAGAGTACAAGCATCAGCGGATTTGTTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
No domains found.

Protein Analysis

506

Amino Acids

55.29

Weight (kDa)

8.82

Isoelectric Point (pI)

45.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1065
AciI CCGC 3 cut(s) 814, 1121, 1503
AcoI YGGCCR 1 cut(s) 805
AcsI RAATTY 2 cut(s) 573, 772
AcuI CTGAAG 3 cut(s) 897, 1047, 1194
AdeI CACNNNGTG 1 cut(s) 730
AfaI GTAC 2 cut(s) 219, 1492
AfeI AGCGCT 1 cut(s) 445
AfiI CCNNNNNNNGG 5 cut(s) 50, 131, 254, 814, 1304
AflII CTTAAG 1 cut(s) 583
AjnI CCWGG 2 cut(s) 124, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 1386, 1418
AloI GAACNNNNNNTCC 4 cut(s) 1209, 1241, 1357, 1389
Alw21I GWGCWC 2 cut(s) 388, 1268
Alw26I GTCTC 1 cut(s) 1468
Aor51HI AGCGCT 1 cut(s) 445
AoxI GGCC 5 cut(s) 805, 865, 1133, 1439, 1453
ApaI GGGCCC 1 cut(s) 1443
ApeKI GCWGC 6 cut(s) 182, 244, 304, 446, 554, 1093
ApoI RAATTY 2 cut(s) 573, 772
ArsI GACNNNNNNTTYG 2 cut(s) 1417, 1449
AseI ATTAAT 1 cut(s) 261
Asp700I GAANNNNTTC 1 cut(s) 576
AspLEI GCGC 2 cut(s) 446, 1066
AspS9I GGNCC 3 cut(s) 1425, 1439, 1440
AsuHPI GGTGA 3 cut(s) 146, 162, 467
AvaII GGWCC 1 cut(s) 1425
BaeGI GKGCMC 1 cut(s) 1443
BalI TGGCCA 1 cut(s) 807
BanII GRGCYC 2 cut(s) 1268, 1443
BarI GAAGNNNNNNTAC 4 cut(s) 870, 902, 1020, 1052
Bbv12I GWGCWC 2 cut(s) 388, 1268
BbvI GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1105
BccI CCATC 6 cut(s) 344, 504, 797, 815, 1006, 1144
BciT130I CCWGG 2 cut(s) 126, 1086
BciVI GTATCC 2 cut(s) 474, 1042
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 1468
BfaI CTAG 2 cut(s) 12, 687
BfmI CTRYAG 1 cut(s) 696
BfoI RGCGCY 1 cut(s) 447
BfrI CTTAAG 1 cut(s) 583
BfuI GTATCC 2 cut(s) 474, 1042
BisI GCNGC 6 cut(s) 183, 245, 305, 447, 555, 1094
BlsI GCNGC 6 cut(s) 184, 246, 306, 448, 556, 1095
Bme1390I CCNGG 2 cut(s) 126, 1086
Bme18I GGWCC 1 cut(s) 1425
BmgT120I GGNCC 3 cut(s) 1425, 1439, 1440
BmiI GGNNCC 4 cut(s) 42, 1400, 1440, 1441
BmrFI CCNGG 2 cut(s) 126, 1086
BmsI GCATC 8 cut(s) 336, 514, 775, 890, 1026, 1159, 1425, 1506
BplI GAGNNNNNCTC 2 cut(s) 710, 742
BsaBI GATNNNNATC 1 cut(s) 789
BsaJI CCNNGG 2 cut(s) 1302, 1402
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 131, 254, 814, 1304
Bse1I ACTGG 3 cut(s) 461, 534, 840
Bse3DI GCAATG 1 cut(s) 184
Bse8I GATNNNNATC 1 cut(s) 789
BseBI CCWGG 2 cut(s) 126, 1086
BseDI CCNNGG 2 cut(s) 1302, 1402
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 5 cut(s) 50, 131, 254, 814, 1304
BseMI GCAATG 1 cut(s) 184
BseNI ACTGG 3 cut(s) 461, 534, 840
BseSI GKGCMC 1 cut(s) 1443
BseXI GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1105
BsgI GTGCAG 1 cut(s) 558
BshFI GGCC 5 cut(s) 807, 867, 1135, 1441, 1455
BsiHKAI GWGCWC 2 cut(s) 388, 1268
BslI CCNNNNNNNGG 5 cut(s) 50, 131, 254, 814, 1304
BsmAI GTCTC 1 cut(s) 1468
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 5 cut(s) 807, 867, 1135, 1441, 1455
Bsp120I GGGCCC 1 cut(s) 1439
Bsp1286I GDGCHC 3 cut(s) 388, 1268, 1443
Bsp1407I TGTACA 1 cut(s) 217
Bsp143I GATC 4 cut(s) 33, 294, 790, 1447
BspACI CCGC 3 cut(s) 814, 1121, 1503
BspANI GGCC 5 cut(s) 807, 867, 1135, 1441, 1455
BspHI TCATGA 1 cut(s) 1144
BspLI GGNNCC 4 cut(s) 42, 1400, 1440, 1441
BspMAI CTGCAG 1 cut(s) 700
BspTI CTTAAG 1 cut(s) 583
BsrDI GCAATG 1 cut(s) 184
BsrGI TGTACA 1 cut(s) 217
BsrI ACTGG 3 cut(s) 461, 534, 840
BssECI CCNNGG 2 cut(s) 1302, 1402
BssMI GATC 4 cut(s) 33, 294, 790, 1447
BssT1I CCWWGG 2 cut(s) 1302, 1402
Bst2UI CCWGG 2 cut(s) 126, 1086
Bst4CI ACNGT 4 cut(s) 113, 222, 491, 850
Bst6I CTCTTC 2 cut(s) 1286, 1299
BstAFI CTTAAG 1 cut(s) 583
BstAPI GCANNNNNTGC 1 cut(s) 704
BstAUI TGTACA 1 cut(s) 217
BstC8I GCNNGC 4 cut(s) 1091, 1098, 1338, 1457
BstDEI CTNAG 2 cut(s) 380, 894
BstH2I RGCGCY 1 cut(s) 447
BstHHI GCGC 2 cut(s) 446, 1066
BstKTI GATC 4 cut(s) 36, 297, 793, 1450
BstMAI GTCTC 1 cut(s) 1468
BstMBI GATC 4 cut(s) 33, 294, 790, 1447
BstMWI GCNNNNNNNGC 6 cut(s) 432, 441, 704, 1014, 1086, 1342
BstNI CCWGG 2 cut(s) 126, 1086
BstNSI RCATGY 3 cut(s) 833, 1093, 1340
BstSCI CCNGG 2 cut(s) 124, 1084
BstSFI CTRYAG 1 cut(s) 696
BstSLI GKGCMC 1 cut(s) 1443
BstV1I GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1105
BstXI CCANNNNNNTGG 1 cut(s) 1268
BsuI GTATCC 2 cut(s) 474, 1042
BsuRI GGCC 5 cut(s) 807, 867, 1135, 1441, 1455
BtsIMutI CAGTG 5 cut(s) 109, 227, 496, 541, 833
Cac8I GCNNGC 4 cut(s) 1091, 1098, 1338, 1457
CciI TCATGA 1 cut(s) 1144
CfoI GCGC 2 cut(s) 446, 1066
Cfr13I GGNCC 3 cut(s) 1425, 1439, 1440
Csp6I GTAC 2 cut(s) 218, 1491
CviAII CATG 6 cut(s) 73, 830, 1090, 1145, 1187, 1337
CviQI GTAC 2 cut(s) 218, 1491
DdeI CTNAG 2 cut(s) 380, 894
DpnI GATC 4 cut(s) 35, 296, 792, 1449
DpnII GATC 4 cut(s) 33, 294, 790, 1447
DraIII CACNNNGTG 1 cut(s) 730
EaeI YGGCCR 1 cut(s) 805
Eam1104I CTCTTC 2 cut(s) 1286, 1299
EarI CTCTTC 2 cut(s) 1286, 1299
Ecl136II GAGCTC 1 cut(s) 1266
Eco130I CCWWGG 2 cut(s) 1302, 1402
Eco147I AGGCCT 1 cut(s) 867
Eco24I GRGCYC 2 cut(s) 1268, 1443
Eco47I GGWCC 1 cut(s) 1425
Eco47III AGCGCT 1 cut(s) 445
Eco53kI GAGCTC 1 cut(s) 1266
Eco57I CTGAAG 3 cut(s) 897, 1047, 1194
EcoICRI GAGCTC 1 cut(s) 1266
EcoO109I RGGNCCY 2 cut(s) 1439, 1440
EcoRII CCWGG 2 cut(s) 124, 1084
EcoT14I CCWWGG 2 cut(s) 1302, 1402
EcoT22I ATGCAT 2 cut(s) 709, 1192
EcoT38I GRGCYC 2 cut(s) 1268, 1443
ErhI CCWWGG 2 cut(s) 1302, 1402
FaeI CATG 6 cut(s) 76, 833, 1093, 1148, 1190, 1340
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FatI CATG 6 cut(s) 72, 829, 1089, 1144, 1186, 1336
FauI CCCGC 1 cut(s) 807
FauNDI CATATG 1 cut(s) 709
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 6 cut(s) 183, 245, 305, 447, 555, 1094
FriOI GRGCYC 2 cut(s) 1268, 1443
Fsp4HI GCNGC 6 cut(s) 183, 245, 305, 447, 555, 1094
FspBI CTAG 2 cut(s) 12, 687
FspI TGCGCA 1 cut(s) 1065
GlaI GCGC 2 cut(s) 445, 1065
GluI GCNGC 6 cut(s) 183, 245, 305, 447, 555, 1094
HaeII RGCGCY 1 cut(s) 447
HaeIII GGCC 5 cut(s) 807, 867, 1135, 1441, 1455
HhaI GCGC 2 cut(s) 446, 1066
Hin1II CATG 6 cut(s) 76, 833, 1093, 1148, 1190, 1340
Hin6I GCGC 2 cut(s) 444, 1064
HinP1I GCGC 2 cut(s) 444, 1064
HincII GTYRAC 2 cut(s) 655, 735
HindII GTYRAC 2 cut(s) 655, 735
HinfI GANTC 5 cut(s) 752, 965, 1148, 1349, 1430
HpaI GTTAAC 1 cut(s) 735
HphI GGTGA 3 cut(s) 146, 162, 467
Hpy166II GTNNAC 3 cut(s) 655, 735, 827
Hpy188I TCNGA 8 cut(s) 211, 332, 622, 667, 877, 1027, 1114, 1238
Hpy188III TCNNGA 2 cut(s) 718, 1145
Hpy8I GTNNAC 3 cut(s) 655, 735, 827
HpyAV CCTTC 3 cut(s) 317, 639, 922
HpyCH4III ACNGT 4 cut(s) 113, 222, 491, 850
HpyF10VI GCNNNNNNNGC 6 cut(s) 432, 441, 704, 1014, 1086, 1342
HpyF3I CTNAG 2 cut(s) 380, 894
Hsp92II CATG 6 cut(s) 76, 833, 1093, 1148, 1190, 1340
HspAI GCGC 2 cut(s) 444, 1064
Ksp22I TGATCA 1 cut(s) 33
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 4 cut(s) 33, 294, 790, 1447
LmnI GCTCC 2 cut(s) 13, 1398
Lsp1109I GCAGC 6 cut(s) 194, 231, 316, 433, 566, 1105
LweI GCATC 8 cut(s) 336, 514, 775, 890, 1026, 1159, 1425, 1506
MaeI CTAG 2 cut(s) 12, 687
MaeIII GTNAC 2 cut(s) 134, 850
MalI GATC 4 cut(s) 35, 296, 792, 1449
MboI GATC 4 cut(s) 33, 294, 790, 1447
MboII GAAGA 9 cut(s) 136, 272, 393, 403, 406, 519, 1187, 1273, 1286
MhlI GDGCHC 3 cut(s) 388, 1268, 1443
MlsI TGGCCA 1 cut(s) 807
MluCI AATT 6 cut(s) 299, 573, 772, 1107, 1161, 1369
MluNI TGGCCA 1 cut(s) 807
MlyI GAGTC 1 cut(s) 1424
Mox20I TGGCCA 1 cut(s) 807
Mph1103I ATGCAT 2 cut(s) 709, 1192
MroXI GAANNNNTTC 1 cut(s) 576
MscI TGGCCA 1 cut(s) 807
MseI TTAA 4 cut(s) 261, 584, 734, 1512
Msp20I TGGCCA 1 cut(s) 807
MspA1I CMGCKG 1 cut(s) 1503
MspCI CTTAAG 1 cut(s) 583
MspR9I CCNGG 2 cut(s) 126, 1086
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 2 cut(s) 126, 1086
MwoI GCNNNNNNNGC 6 cut(s) 432, 441, 704, 1014, 1086, 1342
NdeI CATATG 1 cut(s) 709
NdeII GATC 4 cut(s) 33, 294, 790, 1447
NlaIII CATG 6 cut(s) 76, 833, 1093, 1148, 1190, 1340
NlaIV GGNNCC 4 cut(s) 42, 1400, 1440, 1441
NmuCI GTSAC 1 cut(s) 134
NsbI TGCGCA 1 cut(s) 1065
NsiI ATGCAT 2 cut(s) 709, 1192
NspI RCATGY 3 cut(s) 833, 1093, 1340
PaeI GCATGC 2 cut(s) 1093, 1340
PagI TCATGA 1 cut(s) 1144
PceI AGGCCT 1 cut(s) 867
PctI GAATGC 1 cut(s) 709
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 4 cut(s) 752, 965, 1148, 1349
PkrI GCNGC 6 cut(s) 184, 246, 306, 448, 556, 1095
PleI GAGTC 1 cut(s) 1424
PpsI GAGTC 1 cut(s) 1424
PshBI ATTAAT 1 cut(s) 261
Psp124BI GAGCTC 1 cut(s) 1268
Psp6I CCWGG 2 cut(s) 124, 1084
PspGI CCWGG 2 cut(s) 124, 1084
PspN4I GGNNCC 4 cut(s) 42, 1400, 1440, 1441
PspOMI GGGCCC 1 cut(s) 1439
PspPI GGNCC 3 cut(s) 1425, 1439, 1440
PsrI GAACNNNNNNTAC 2 cut(s) 581, 613
PstI CTGCAG 1 cut(s) 700
RsaI GTAC 2 cut(s) 219, 1492
RsaNI GTAC 2 cut(s) 218, 1491
SacI GAGCTC 1 cut(s) 1268
SaqAI TTAA 4 cut(s) 261, 584, 734, 1512
SatI GCNGC 6 cut(s) 183, 245, 305, 447, 555, 1094
Sau3AI GATC 4 cut(s) 33, 294, 790, 1447
Sau96I GGNCC 3 cut(s) 1425, 1439, 1440
SchI GAGTC 1 cut(s) 1424
ScrFI CCNGG 2 cut(s) 126, 1086
SduI GDGCHC 3 cut(s) 388, 1268, 1443
SfaNI GCATC 8 cut(s) 336, 514, 775, 890, 1026, 1159, 1425, 1506
SfcI CTRYAG 1 cut(s) 696
SinI GGWCC 1 cut(s) 1425
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
SphI GCATGC 2 cut(s) 1093, 1340
Sse9I AATT 6 cut(s) 299, 573, 772, 1107, 1161, 1369
SseBI AGGCCT 1 cut(s) 867
SsiI CCGC 3 cut(s) 814, 1121, 1503
SspMI CTAG 2 cut(s) 12, 687
SstI GAGCTC 1 cut(s) 1268
StuI AGGCCT 1 cut(s) 867
StyD4I CCNGG 2 cut(s) 124, 1084
StyI CCWWGG 2 cut(s) 1302, 1402
TaaI ACNGT 4 cut(s) 113, 222, 491, 850
TaqI TCGA 2 cut(s) 717, 1256
TaqII GACCGA 1 cut(s) 1442
TasI AATT 6 cut(s) 299, 573, 772, 1107, 1161, 1369
TatI WGTACW 2 cut(s) 217, 1490
TfiI GAWTC 4 cut(s) 752, 965, 1148, 1349
Tru1I TTAA 4 cut(s) 261, 584, 734, 1512
Tru9I TTAA 4 cut(s) 261, 584, 734, 1512
TscAI CASTG 5 cut(s) 116, 227, 496, 541, 840
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 6 cut(s) 182, 244, 304, 446, 554, 1093
Tsp45I GTSAC 1 cut(s) 134
TspGWI ACGGA 4 cut(s) 403, 934, 1378, 1411
TspRI CASTG 5 cut(s) 116, 227, 496, 541, 840
Vha464I CTTAAG 1 cut(s) 583
VpaK11BI GGWCC 1 cut(s) 1425
VspI ATTAAT 1 cut(s) 261
XapI RAATTY 2 cut(s) 573, 772
XceI RCATGY 3 cut(s) 833, 1093, 1340
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 2 cut(s) 12, 687
Zsp2I ATGCAT 2 cut(s) 709, 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.