MD01G1014700.v1.1

TSL-kinase interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007256
Physical Location & Seq
Forward (+)
6897323 .. 6900214
2892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1014700.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGAAAGCTTCTAGAGAAACAAACAGGAAGGTGATCAAGATTCCTGTAGAGGGAAGAGCACGTATAGGAAGTACCATGAGAGAACAGAGATTTACAGGGAGAACTACCCGAGTCTGCCACAAAGCAACAGGGCAAGGTGGCAATTTCTTGGTTGAAATGGACGAGAATCCCGAAGGCATGTGTCTGGAGCTTCCTGACATAGGTGTGCCCTTTTTAGGAACAATAAAGAGACTGCCTGTTCTTGAGTGTTATCAAGGGCAAAAACTTCTTCCGCAGTCAAAGATCAAGTTGCAGCTTTTCCCTGTAAATGAAGCCACCCGCATAGGATTGGAAAAGGATGGACATCATCCATATTTAGAACTCATTCTTAGACCACGGAAGAAGATTACTTCTGTACTAAAGCACCTTAATGATAAGTGGGGCAGATCAAGCATTGCTTTTGAATGGACACTGAATGATGGCAATATTAGTGCCGGAGCGGTCTATGCAGCTTTTGGAAGCCCTGCAGTTTTTCGCTTAAGGTATGGCTGGTTCTCTACTGAACCTAAAACTGCTTATCAACCTTCTACATCAGCTAATGACTTACGATCTGAAGGCGAGCAGAGATGTAGAAGCCCCGCAACAGAGAATGTTATTGATGAATTATGGAAACATACTGCTTTAGCAACAAAAGATTTCAAATCAAATGATGCAAGTGAAACAATAAATGCAGATACTGAGAAGATTTCTACTGGAGCTGTTGATCCCAGGGACAATATTCAGAGGAGGATGGGTAACCATGCTCAGGAACCACTTTTGATTGACAGTGTAACTAATATAAGCGTTGGAGGCCTACTTTCTGAAGCATCCTTACAGGCCAAGTTCAATAGCAGTAAAGAAAAACAAATTGGGAAAAATGAAGGCTTTTATCCTACTGAAATAAAAAATGATTCAAAGTCATTTGTGTCGTGGGATTGTCCATCTATGAGCATTGGTGGCCTCCTGTCTGAAGCATCCTTGCAGGGCAAGCTCAATGGATCTGATGCACAACCAGTTGAGAGCAATGCAGGCTTGCCACCAACTCAAGGTCAAAGGCCATCCACTCATGACTCATGCTTATCTATTTTTGACGCCAAAGAAACGTGCCGTGCATTTCCTTCTCAAAAGTTCTCTTCGTCGGGAAAAGATGTTCTGGGGTTTGGTGGAAGTCGTGGATGTTTCAACCAGAATCCTGTTTCTAAACTATTCAAGTTCCCTGATGAAGCCAAGGTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
No domains found.

Protein Analysis

419

Amino Acids

46.0

Weight (kDa)

8.83

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 479
AciI CCGC 4 cut(s) 272, 319, 479, 618
AclWI GGATC 2 cut(s) 737, 1024
AcuI CTGAAG 3 cut(s) 612, 861, 1008
AcyI GRCGYC 1 cut(s) 1110
AfaI GTAC 2 cut(s) 73, 396
AfiI CCNNNNNNNGG 5 cut(s) 50, 200, 215, 784, 1064
AflII CTTAAG 1 cut(s) 517
AgsI TTSAA 7 cut(s) 155, 443, 679, 865, 933, 1201, 1228
AjnI CCWGG 1 cut(s) 746
AjuI GAANNNNNNNTTGG 2 cut(s) 870, 902
AloI GAACNNNNNNTCC 2 cut(s) 1152, 1184
AluBI AGCT 7 cut(s) 8, 190, 295, 491, 575, 737, 1009
AluI AGCT 7 cut(s) 8, 190, 295, 491, 575, 737, 1009
Alw21I GWGCWC 1 cut(s) 61
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 2 cut(s) 737, 1024
AlwNI CAGNNNCTG 1 cut(s) 716
Ama87I CYCGRG 1 cut(s) 108
AoxI GGCC 4 cut(s) 829, 855, 976, 1073
ApeKI GCWGC 2 cut(s) 292, 488
Asp700I GAANNNNTTC 1 cut(s) 363
AsuHPI GGTGA 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 108
BaeGI GKGCMC 1 cut(s) 210
BarI GAAGNNNNNNTAC 2 cut(s) 834, 866
Bbv12I GWGCWC 1 cut(s) 61
BbvI GCAGC 2 cut(s) 304, 500
BccI CCATC 5 cut(s) 332, 452, 763, 967, 1084
BceAI ACGGC 1 cut(s) 1110
BciT130I CCWGG 1 cut(s) 748
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 2 cut(s) 45, 504
BfrI CTTAAG 1 cut(s) 517
BisI GCNGC 2 cut(s) 293, 489
BlsI GCNGC 2 cut(s) 294, 490
Bme1390I CCNGG 1 cut(s) 748
BmeT110I CYCGRG 1 cut(s) 108
BmiI GGNNCC 1 cut(s) 789
BmrFI CCNGG 1 cut(s) 748
BmsI GCATC 4 cut(s) 679, 854, 1001, 1012
BpmI CTGGAG 2 cut(s) 206, 753
Bpu10I CCTNAGC 1 cut(s) 783
BpuEI CTTGAG 2 cut(s) 263, 1047
BsaAI YACGTR 1 cut(s) 62
BsaBI GATNNNNATC 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 1110
BsaJI CCNNGG 4 cut(s) 374, 746, 747, 1245
BsaXI ACNNNNNCTCC 2 cut(s) 757, 787
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 200, 215, 784, 1064
Bse1I ACTGG 2 cut(s) 736, 1031
Bse3DI GCAATG 2 cut(s) 432, 1048
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 1 cut(s) 748
BseDI CCNNGG 4 cut(s) 374, 746, 747, 1245
BseGI GGATG 7 cut(s) 343, 346, 774, 845, 992, 1076, 1199
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 5 cut(s) 50, 200, 215, 784, 1064
BseMI GCAATG 2 cut(s) 432, 1048
BseMII CTCAG 2 cut(s) 708, 797
BseNI ACTGG 2 cut(s) 736, 1031
BseRI GAGGAG 1 cut(s) 778
BseSI GKGCMC 1 cut(s) 210
BseXI GCAGC 2 cut(s) 304, 500
BshFI GGCC 4 cut(s) 831, 857, 978, 1075
BsiHKAI GWGCWC 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 108
BsiSI CCGG 1 cut(s) 474
BslFI GGGAC 1 cut(s) 764
BslI CCNNNNNNNGG 5 cut(s) 50, 200, 215, 784, 1064
BsmAI GTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 764
BsnI GGCC 4 cut(s) 831, 857, 978, 1075
BsoBI CYCGRG 1 cut(s) 108
Bsp1286I GDGCHC 2 cut(s) 61, 210
Bsp143I GATC 6 cut(s) 33, 282, 425, 587, 742, 1016
BspACI CCGC 4 cut(s) 272, 319, 479, 618
BspANI GGCC 4 cut(s) 831, 857, 978, 1075
BspCNI CTCAG 2 cut(s) 709, 796
BspHI TCATGA 1 cut(s) 1084
BspLI GGNNCC 1 cut(s) 789
BspMAI CTGCAG 1 cut(s) 508
BspPI GGATC 2 cut(s) 737, 1024
BspQI GCTCTTC 1 cut(s) 49
BspTI CTTAAG 1 cut(s) 517
BsrBI CCGCTC 1 cut(s) 479
BsrDI GCAATG 2 cut(s) 432, 1048
BsrI ACTGG 2 cut(s) 736, 1031
BssECI CCNNGG 4 cut(s) 374, 746, 747, 1245
BssMI GATC 6 cut(s) 33, 282, 425, 587, 742, 1016
BssNI GRCGYC 1 cut(s) 1110
BssT1I CCWWGG 1 cut(s) 1245
Bst2UI CCWGG 1 cut(s) 748
Bst4CI ACNGT 1 cut(s) 806
Bst6I CTCTTC 2 cut(s) 49, 1156
BstACI GRCGYC 1 cut(s) 1110
BstAFI CTTAAG 1 cut(s) 517
BstBAI YACGTR 1 cut(s) 62
BstC8I GCNNGC 4 cut(s) 599, 1007, 1048, 1052
BstDEI CTNAG 3 cut(s) 368, 717, 783
BstDSI CCRYGG 1 cut(s) 374
BstEII GGTNACC 1 cut(s) 773
BstENI CCTNNNNNAGG 1 cut(s) 198
BstF5I GGATG 7 cut(s) 343, 346, 774, 845, 992, 1076, 1199
BstKTI GATC 6 cut(s) 36, 285, 428, 590, 745, 1019
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 6 cut(s) 33, 282, 425, 587, 742, 1016
BstMWI GCNNNNNNNGC 6 cut(s) 429, 485, 828, 975, 1006, 1047
BstNI CCWGG 1 cut(s) 748
BstNSI RCATGY 1 cut(s) 181
BstPI GGTNACC 1 cut(s) 773
BstSCI CCNGG 1 cut(s) 746
BstSFI CTRYAG 2 cut(s) 45, 504
BstSLI GKGCMC 1 cut(s) 210
BstV1I GCAGC 2 cut(s) 304, 500
BstX2I RGATCY 1 cut(s) 1016
BstYI RGATCY 1 cut(s) 1016
BsuRI GGCC 4 cut(s) 831, 857, 978, 1075
BtgI CCRYGG 1 cut(s) 374
BtsCI GGATG 7 cut(s) 343, 346, 774, 845, 992, 1076, 1199
BtsIMutI CAGTG 2 cut(s) 449, 811
Cac8I GCNNGC 4 cut(s) 599, 1007, 1048, 1052
CaiI CAGNNNCTG 1 cut(s) 716
CciI TCATGA 1 cut(s) 1084
CseI GACGC 1 cut(s) 1118
Csp6I GTAC 2 cut(s) 72, 395
CviAII CATG 5 cut(s) 76, 178, 779, 1085, 1092
CviQI GTAC 2 cut(s) 72, 395
DdeI CTNAG 3 cut(s) 368, 717, 783
DpnI GATC 6 cut(s) 35, 284, 427, 589, 744, 1018
DpnII GATC 6 cut(s) 33, 282, 425, 587, 742, 1016
Eam1104I CTCTTC 2 cut(s) 49, 1156
EarI CTCTTC 2 cut(s) 49, 1156
Eco130I CCWWGG 1 cut(s) 1245
Eco147I AGGCCT 1 cut(s) 831
Eco57I CTGAAG 3 cut(s) 612, 861, 1008
Eco88I CYCGRG 1 cut(s) 108
Eco91I GGTNACC 1 cut(s) 773
EcoNI CCTNNNNNAGG 1 cut(s) 198
EcoO65I GGTNACC 1 cut(s) 773
EcoRII CCWGG 1 cut(s) 746
EcoT14I CCWWGG 1 cut(s) 1245
ErhI CCWWGG 1 cut(s) 1245
FaeI CATG 5 cut(s) 79, 181, 782, 1088, 1095
FalI AAGNNNNNCTT 2 cut(s) 421, 453
FaqI GGGAC 1 cut(s) 764
FatI CATG 5 cut(s) 75, 177, 778, 1084, 1091
FauI CCCGC 2 cut(s) 326, 625
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 2 cut(s) 293, 489
FokI GGATG 7 cut(s) 333, 350, 781, 832, 979, 1063, 1206
Fsp4HI GCNGC 2 cut(s) 293, 489
FspBI CTAG 1 cut(s) 12
GluI GCNGC 2 cut(s) 293, 489
GsuI CTGGAG 2 cut(s) 206, 753
HaeIII GGCC 4 cut(s) 831, 857, 978, 1075
HapII CCGG 1 cut(s) 474
HgaI GACGC 1 cut(s) 1118
Hin1I GRCGYC 1 cut(s) 1110
Hin1II CATG 5 cut(s) 79, 181, 782, 1088, 1095
HindIII AAGCTT 1 cut(s) 6
HinfI GANTC 6 cut(s) 40, 111, 166, 929, 1088, 1207
HpaII CCGG 1 cut(s) 474
HphI GGTGA 1 cut(s) 43
Hpy188I TCNGA 5 cut(s) 592, 762, 841, 988, 1021
Hpy188III TCNNGA 9 cut(s) 12, 37, 170, 185, 194, 242, 785, 1085, 1158
Hpy99I CGWCG 1 cut(s) 1159
HpyAV CCTTC 6 cut(s) 22, 167, 573, 587, 893, 1146
HpyCH4III ACNGT 1 cut(s) 806
HpyCH4IV ACGT 2 cut(s) 61, 1121
HpyCH4V TGCA 9 cut(s) 292, 488, 506, 692, 710, 1000, 1025, 1046, 1130
HpyF10VI GCNNNNNNNGC 6 cut(s) 429, 485, 828, 975, 1006, 1047
HpyF3I CTNAG 3 cut(s) 368, 717, 783
HpySE526I ACGT 2 cut(s) 61, 1121
Hsp92I GRCGYC 1 cut(s) 1110
Hsp92II CATG 5 cut(s) 79, 181, 782, 1088, 1095
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 6 cut(s) 33, 282, 425, 587, 742, 1016
LguI GCTCTTC 1 cut(s) 49
LmnI GCTCC 3 cut(s) 187, 476, 734
Lsp1109I GCAGC 2 cut(s) 304, 500
LweI GCATC 4 cut(s) 679, 854, 1001, 1012
MaeI CTAG 1 cut(s) 12
MaeII ACGT 2 cut(s) 61, 1121
MaeIII GTNAC 2 cut(s) 773, 808
MalI GATC 6 cut(s) 35, 284, 427, 589, 744, 1018
MbiI CCGCTC 1 cut(s) 479
MboI GATC 6 cut(s) 33, 282, 425, 587, 742, 1016
MboII GAAGA 6 cut(s) 66, 260, 391, 394, 733, 1143
MflI RGATCY 1 cut(s) 1016
MhlI GDGCHC 2 cut(s) 61, 210
MluCI AATT 3 cut(s) 142, 641, 885
MlyI GAGTC 2 cut(s) 120, 1082
MmeI TCCRAC 1 cut(s) 805
MnlI CCTC 5 cut(s) 43, 756, 759, 821, 989
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 2 cut(s) 408, 518
MslI CAYNNNNRTG 2 cut(s) 203, 408
MspCI CTTAAG 1 cut(s) 517
MspI CCGG 1 cut(s) 474
MspR9I CCNGG 1 cut(s) 748
MvaI CCWGG 1 cut(s) 748
MwoI GCNNNNNNNGC 6 cut(s) 429, 485, 828, 975, 1006, 1047
NdeII GATC 6 cut(s) 33, 282, 425, 587, 742, 1016
NlaIII CATG 5 cut(s) 79, 181, 782, 1088, 1095
NlaIV GGNNCC 1 cut(s) 789
NspI RCATGY 1 cut(s) 181
PagI TCATGA 1 cut(s) 1084
PasI CCCWGGG 1 cut(s) 747
PceI AGGCCT 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 49
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 4 cut(s) 40, 166, 929, 1207
PkrI GCNGC 2 cut(s) 294, 490
PleI GAGTC 2 cut(s) 119, 1082
PpsI GAGTC 2 cut(s) 119, 1082
Ppu21I YACGTR 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 746
PspEI GGTNACC 1 cut(s) 773
PspGI CCWGG 1 cut(s) 746
PspN4I GGNNCC 1 cut(s) 789
PsrI GAACNNNNNNTAC 2 cut(s) 515, 547
PstI CTGCAG 1 cut(s) 508
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 1 cut(s) 1016
RsaI GTAC 2 cut(s) 73, 396
RsaNI GTAC 2 cut(s) 72, 395
RseI CAYNNNNRTG 2 cut(s) 203, 408
SapI GCTCTTC 1 cut(s) 49
SaqAI TTAA 2 cut(s) 408, 518
SatI GCNGC 2 cut(s) 293, 489
Sau3AI GATC 6 cut(s) 33, 282, 425, 587, 742, 1016
SchI GAGTC 2 cut(s) 120, 1082
ScrFI CCNGG 1 cut(s) 748
SduI GDGCHC 2 cut(s) 61, 210
SfaNI GCATC 4 cut(s) 679, 854, 1001, 1012
SfcI CTRYAG 2 cut(s) 45, 504
SmiMI CAYNNNNRTG 2 cut(s) 203, 408
SmlI CTYRAG 3 cut(s) 242, 517, 1062
SmoI CTYRAG 3 cut(s) 242, 517, 1062
Sse9I AATT 3 cut(s) 142, 641, 885
SseBI AGGCCT 1 cut(s) 831
SsiI CCGC 4 cut(s) 272, 319, 479, 618
SspI AATATT 2 cut(s) 466, 757
SspMI CTAG 1 cut(s) 12
StuI AGGCCT 1 cut(s) 831
StyD4I CCNGG 1 cut(s) 746
StyI CCWWGG 1 cut(s) 1245
TaaI ACNGT 1 cut(s) 806
TaiI ACGT 2 cut(s) 64, 1124
TasI AATT 3 cut(s) 142, 641, 885
TatI WGTACW 1 cut(s) 394
TfiI GAWTC 4 cut(s) 40, 166, 929, 1207
Tru1I TTAA 2 cut(s) 408, 518
Tru9I TTAA 2 cut(s) 408, 518
TscAI CASTG 2 cut(s) 456, 811
TseI GCWGC 2 cut(s) 292, 488
TspDTI ATGAA 5 cut(s) 17, 324, 654, 912, 1254
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 2 cut(s) 456, 811
Vha464I CTTAAG 1 cut(s) 517
XagI CCTNNNNNAGG 1 cut(s) 198
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 1 cut(s) 181
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.