AT4G39810

Exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015333
Physical Location & Seq
Reverse (-)
18474481 .. 18475804
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G39810.1

Sequence Viewer

Length: 768 bp
ATGGCGGTCCAAACATTTCCCAACGAAATCGTCTTCTTCGATCTAGAAACAAACGTACCAAACAAAGCAGGGCAACATTTTCACATACTAGAGTTTGGGGCGATCATCGTATGTCCAAAAAAACTCGAGGAGCTAGAGAGCTTCACAACGCTTATACAACCTAAAGACTTGTCGGTGGTCTCAATAAGGTCGTCTAGGTCAGATGGGATCACCAGGGCTAAGGTTACGAACGCACCAAGTTTTGAAGACGTGGCTGAGAAAATCCATGGTTTGTTGAACGGTAGGATTTGGGCAGGTCATAACATTAGAAGATTTGATTGCGTTAGGATCAAAGAAGCTTTCGCTGAGATCGGTAAAGCTGCGCCTGAACCCTCAGGGATCATTGATTCTTTGGGGTTATTAAGTGACAAGTTTGGCAAAAGAGCTGGTAACATGAAGATGGCAAGTTTGGCTGCCTATTTTGGTCTTGGAGTTCAAAAGCACAGGAGTCTCGATGATGTTCGAATGAATTTAGAGGTCCTCAAGCATTGTGCAACAGTGCTTTTCTTGGAATCTACACTTCCCAATCATTTGGAAGGAAAATGGCACACTTCTTCTAAGATTATGACAAGAAGTCGAAGGAATTACCAAATAGCCCAAAGGGCAATGCCTTACTCGAAGGGTAGTCTCGAGAAGATGACACAAAATGTGAAGAATCTGTTAAGCAAAGCTCAAGGCAATCAAACTCTCCAAAGCTTGATCAATCATTCTCATTCTTTGCTTAGATGA

Protein Analysis

255

Amino Acids

28.51

Weight (kDa)

9.62

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 11 - 174 2.8e-34 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39810
fragaria_vesca FvH4_1g21490
malus_domestica MD15G1329100.v1.1
prunus_persica Prupe.6G179800_v2.0.a1
pyrus_communis pycom01g05230 pycom15g29710 pycom15g29720
rosa_chinensis RchiOBHm_Chr2g0115371
rosa_laevigata RLG00000018180
rosa_roxburghii Rroxscaffold_2G00130860
rosa_rugosa Rorug02G0195900
rosa_samantha Rh2AG254300 Rh2BG264500 Rh2CG259000 Rh2DG262400
rosa_wichuraiana Rw2G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 284
AciI CCGC 1 cut(s) 5
AclWI GGATC 3 cut(s) 215, 335, 386
AcsI RAATTY 1 cut(s) 508
AfaI GTAC 1 cut(s) 57
AgsI TTSAA 3 cut(s) 245, 277, 476
AhdI GACNNNNNGTC 1 cut(s) 612
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 1 cut(s) 212
AluBI AGCT 7 cut(s) 133, 141, 338, 359, 425, 710, 735
AluI AGCT 7 cut(s) 133, 141, 338, 359, 425, 710, 735
Alw26I GTCTC 3 cut(s) 184, 494, 671
AlwI GGATC 3 cut(s) 215, 335, 386
Ama87I CYCGRG 2 cut(s) 125, 668
ApeKI GCWGC 2 cut(s) 359, 452
ApoI RAATTY 1 cut(s) 508
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 2 cut(s) 7, 517
AsuHPI GGTGA 1 cut(s) 202
AsuII TTCGAA 1 cut(s) 502
AvaI CYCGRG 2 cut(s) 125, 668
AvaII GGWCC 2 cut(s) 7, 517
AxyI CCTNAGG 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 39, 72
BbsI GAAGAC 2 cut(s) 25, 252
BbvI GCAGC 2 cut(s) 346, 439
BccI CCATC 2 cut(s) 197, 433
BciT130I CCWGG 1 cut(s) 214
BclI TGATCA 1 cut(s) 738
BcoDI GTCTC 3 cut(s) 184, 494, 671
BfaI CTAG 4 cut(s) 44, 89, 134, 195
BfuAI ACCTGC 1 cut(s) 284
BglI GCCNNNNNGGC 1 cut(s) 641
BisI GCNGC 2 cut(s) 360, 453
BlsI GCNGC 2 cut(s) 361, 454
Bme1390I CCNGG 1 cut(s) 214
Bme18I GGWCC 2 cut(s) 7, 517
BmeRI GACNNNNNGTC 1 cut(s) 612
BmeT110I CYCGRG 2 cut(s) 125, 668
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 7, 517
BmrFI CCNGG 1 cut(s) 214
BpiI GAAGAC 2 cut(s) 25, 252
Bpu10I CCTNAGC 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 502
BpuEI CTTGAG 2 cut(s) 506, 696
BsaI GGTCTC 1 cut(s) 184
BsaJI CCNNGG 2 cut(s) 213, 265
Bse21I CCTNAGG 1 cut(s) 373
Bse3DI GCAATG 1 cut(s) 651
BseBI CCWGG 1 cut(s) 214
BseDI CCNNGG 2 cut(s) 213, 265
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 3 cut(s) 246, 336, 387
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 2 cut(s) 346, 439
BsiHKCI CYCGRG 2 cut(s) 125, 668
BsmAI GTCTC 3 cut(s) 184, 494, 671
Bso31I GGTCTC 1 cut(s) 184
BsoBI CYCGRG 2 cut(s) 125, 668
Bsp119I TTCGAA 1 cut(s) 502
Bsp143I GATC 7 cut(s) 40, 102, 207, 327, 348, 378, 738
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 247, 337, 386
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 3 cut(s) 215, 335, 386
BspT104I TTCGAA 1 cut(s) 502
BspTNI GGTCTC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 651
BssECI CCNNGG 2 cut(s) 213, 265
BssMI GATC 7 cut(s) 40, 102, 207, 327, 348, 378, 738
BssT1I CCWWGG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 214
Bst4CI ACNGT 2 cut(s) 281, 538
BstBI TTCGAA 1 cut(s) 502
BstDEI CTNAG 6 cut(s) 219, 255, 345, 373, 597, 761
BstDSI CCRYGG 1 cut(s) 265
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 7 cut(s) 43, 105, 210, 330, 351, 381, 741
BstMAI GTCTC 3 cut(s) 184, 494, 671
BstMBI GATC 7 cut(s) 40, 102, 207, 327, 348, 378, 738
BstMWI GCNNNNNNNGC 2 cut(s) 449, 641
BstNI CCWGG 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 346, 439
BstV2I GAAGAC 2 cut(s) 25, 252
BstXI CCANNNNNNTGG 1 cut(s) 571
Bsu36I CCTNAGG 1 cut(s) 373
BtgI CCRYGG 1 cut(s) 265
BtrI CACGTC 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 543
BveI ACCTGC 1 cut(s) 284
CfoI GCGC 1 cut(s) 364
Cfr13I GGNCC 2 cut(s) 7, 517
Csp6I GTAC 1 cut(s) 56
CviAII CATG 2 cut(s) 266, 433
CviQI GTAC 1 cut(s) 56
DdeI CTNAG 6 cut(s) 219, 255, 345, 373, 597, 761
DpnI GATC 7 cut(s) 42, 104, 209, 329, 350, 380, 740
DpnII GATC 7 cut(s) 40, 102, 207, 327, 348, 378, 738
DriI GACNNNNNGTC 1 cut(s) 612
Eam1105I GACNNNNNGTC 1 cut(s) 612
Eco130I CCWWGG 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 7, 517
Eco81I CCTNAGG 1 cut(s) 373
Eco88I CYCGRG 2 cut(s) 125, 668
EcoO109I RGGNCCY 1 cut(s) 517
EcoRII CCWGG 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 2 cut(s) 269, 436
FaiI YATR 7 cut(s) 86, 112, 155, 267, 300, 434, 605
FatI CATG 2 cut(s) 265, 432
FbaI TGATCA 1 cut(s) 738
Fnu4HI GCNGC 2 cut(s) 360, 453
Fsp4HI GCNGC 2 cut(s) 360, 453
FspBI CTAG 4 cut(s) 44, 89, 134, 195
GlaI GCGC 1 cut(s) 363
GluI GCNGC 2 cut(s) 360, 453
HhaI GCGC 1 cut(s) 364
Hin1II CATG 2 cut(s) 269, 436
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HindIII AAGCTT 2 cut(s) 336, 733
HinfI GANTC 4 cut(s) 386, 487, 551, 694
HphI GGTGA 1 cut(s) 202
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 4 cut(s) 44, 491, 668, 670
HpyAV CCTTC 3 cut(s) 569, 612, 652
HpyCH4III ACNGT 2 cut(s) 281, 538
HpyCH4IV ACGT 2 cut(s) 54, 249
HpyCH4V TGCA 1 cut(s) 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 641
HpyF3I CTNAG 6 cut(s) 219, 255, 345, 373, 597, 761
HpySE526I ACGT 2 cut(s) 54, 249
Hsp92II CATG 2 cut(s) 269, 436
HspAI GCGC 1 cut(s) 362
Ksp22I TGATCA 1 cut(s) 738
Kzo9I GATC 7 cut(s) 40, 102, 207, 327, 348, 378, 738
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 8 cut(s) 54, 199, 226, 279, 360, 378, 411, 469
Lsp1109I GCAGC 2 cut(s) 346, 439
MaeI CTAG 4 cut(s) 44, 89, 134, 195
MaeII ACGT 2 cut(s) 54, 249
MaeIII GTNAC 3 cut(s) 223, 404, 428
MalI GATC 7 cut(s) 42, 104, 209, 329, 350, 380, 740
MboI GATC 7 cut(s) 40, 102, 207, 327, 348, 378, 738
MboII GAAGA 8 cut(s) 25, 28, 257, 321, 448, 585, 685, 703
MluCI AATT 2 cut(s) 508, 622
MlyI GAGTC 1 cut(s) 496
MnlI CCTC 4 cut(s) 121, 382, 508, 530
MseI TTAA 2 cut(s) 401, 701
MslI CAYNNNNRTG 1 cut(s) 437
MspR9I CCNGG 1 cut(s) 214
MvaI CCWGG 1 cut(s) 214
MwoI GCNNNNNNNGC 2 cut(s) 449, 641
NcoI CCATGG 1 cut(s) 265
NdeII GATC 7 cut(s) 40, 102, 207, 327, 348, 378, 738
NlaIII CATG 2 cut(s) 269, 436
NmuCI GTSAC 1 cut(s) 404
NspV TTCGAA 1 cut(s) 502
PaeR7I CTCGAG 2 cut(s) 125, 668
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PfeI GAWTC 3 cut(s) 386, 551, 694
PkrI GCNGC 2 cut(s) 361, 454
PleI GAGTC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 495
PpuMI RGGWCCY 1 cut(s) 517
Psp5II RGGWCCY 1 cut(s) 517
Psp6I CCWGG 1 cut(s) 212
PspGI CCWGG 1 cut(s) 212
PspPI GGNCC 2 cut(s) 7, 517
PspPPI RGGWCCY 1 cut(s) 517
PspXI VCTCGAGB 1 cut(s) 125
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
RseI CAYNNNNRTG 1 cut(s) 437
SaqAI TTAA 2 cut(s) 401, 701
SatI GCNGC 2 cut(s) 360, 453
Sau3AI GATC 7 cut(s) 40, 102, 207, 327, 348, 378, 738
Sau96I GGNCC 2 cut(s) 7, 517
SchI GAGTC 1 cut(s) 496
ScrFI CCNGG 1 cut(s) 214
Sfr274I CTCGAG 2 cut(s) 125, 668
SfuI TTCGAA 1 cut(s) 502
SinI GGWCC 2 cut(s) 7, 517
SlaI CTCGAG 2 cut(s) 125, 668
SmiMI CAYNNNNRTG 1 cut(s) 437
SmlI CTYRAG 4 cut(s) 125, 521, 668, 711
SmoI CTYRAG 4 cut(s) 125, 521, 668, 711
Sse9I AATT 2 cut(s) 508, 622
SsiI CCGC 1 cut(s) 5
SspMI CTAG 4 cut(s) 44, 89, 134, 195
StyD4I CCNGG 1 cut(s) 212
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 2 cut(s) 281, 538
TaiI ACGT 2 cut(s) 57, 252
TaqI TCGA 7 cut(s) 39, 126, 492, 502, 616, 656, 669
TasI AATT 2 cut(s) 508, 622
TfiI GAWTC 3 cut(s) 386, 551, 694
Tru1I TTAA 2 cut(s) 401, 701
Tru9I TTAA 2 cut(s) 401, 701
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 2 cut(s) 359, 452
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 2 cut(s) 449, 521
TspRI CASTG 1 cut(s) 543
VpaK11BI GGWCC 2 cut(s) 7, 517
XapI RAATTY 1 cut(s) 508
XbaI TCTAGA 1 cut(s) 43
XhoI CTCGAG 2 cut(s) 125, 668
XspI CTAG 4 cut(s) 44, 89, 134, 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.