FvH4_1g21490

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015333
Physical Location & Seq
Reverse (-)
13488463 .. 13490352
1890 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21490.t1

Sequence Viewer

Length: 849 bp
ATGGACAACCCTTCTACATCTTCACCATGCAAAGAAAGCACATCAACAGAGATTGTGTTCTTTGACATAGAAACAAATGTACCCAATAGAGTTGGACAAAGGTTCTGGGTTTTAGAATTTGGGGCAATTGTTGTTTGCCCTCGGAAGCTTGTGGAGCTAGACAGTTATAGCACCCTCATTAGGCCAACAGACTTGTCTGCTGTACCCTCAAGGTCCGGTAGATGCGATGGGATCACCAAGGATGTTGTTGCAAATGCACCCTTGTTTGAGGAAGTTGCAGATAAGATATTCAACATTTTGAATGGTAGAATTTGGGCAGGTCATAACATCCAAAGGTTCGATTGTGCTCGCATTAAGGAGGCGTTTTCGGACATTGGTAGGCCTGCACCTACTCCTGTTGGGATGATAGACTCCTTAGGGGTATTGACTGAGAAGTTTGGCAGAAGAGCTGGCAATATGAAGATGGCATCATTGGCAACTTACTTCAAGCTCGGGCAGCAAAAGCACAGGAGCCTTGAAGATGTTAGGATGAACCTGGAGGTCCTCAAGAACTGTGCAACTGTGCTGTTTCTGGAAGCAAGCCTTCCTAGCGTGTTAAATGGCAACTGGCATGGCTCCCCAACGGTTACAACTCGGAGTAGAAGTAATGGGAAATTGCCATGCCGAGATGAAACAACAAGCCGAAAGTCTCCCCCAACTGCTTCTATTGGATATCAAAGAGCAGTTCCATATGTTCCAAGGGGGAGTTTGGAAAAGGTGACAGAAAGGGTAAAGAATCTACTGTGTAGAGCACAAGGAAACCAGCCTCTTAGTAACATATTGAAGCATTCTCATTCACTACTCCGGTGA

Protein Analysis

283

Amino Acids

31.24

Weight (kDa)

9.5

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 19 - 182 2e-27 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39810
fragaria_vesca FvH4_1g21490
malus_domestica MD15G1329100.v1.1
prunus_persica Prupe.6G179800_v2.0.a1
pyrus_communis pycom01g05230 pycom15g29710 pycom15g29720
rosa_chinensis RchiOBHm_Chr2g0115371
rosa_laevigata RLG00000018180
rosa_roxburghii Rroxscaffold_2G00130860
rosa_rugosa Rorug02G0195900
rosa_samantha Rh2AG254300 Rh2BG264500 Rh2CG259000 Rh2DG262400
rosa_wichuraiana Rw2G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 2 cut(s) 116, 309
AfaI GTAC 2 cut(s) 81, 204
AfiI CCNNNNNNNGG 1 cut(s) 180
AgsI TTSAA 5 cut(s) 292, 301, 487, 518, 823
AjnI CCWGG 1 cut(s) 534
AluBI AGCT 4 cut(s) 148, 157, 449, 490
AluI AGCT 4 cut(s) 148, 157, 449, 490
Alw21I GWGCWC 2 cut(s) 349, 793
Alw26I GTCTC 1 cut(s) 693
AlwI GGATC 1 cut(s) 239
Ama87I CYCGRG 1 cut(s) 491
AoxI GGCC 2 cut(s) 182, 380
ApeKI GCWGC 1 cut(s) 496
ApoI RAATTY 2 cut(s) 116, 309
AspS9I GGNCC 2 cut(s) 213, 541
AsuHPI GGTGA 3 cut(s) 15, 226, 769
AvaI CYCGRG 1 cut(s) 491
AvaII GGWCC 2 cut(s) 213, 541
AxyI CCTNAGG 1 cut(s) 415
BaeI ACNNNNGTAYC 2 cut(s) 63, 96
Bbv12I GWGCWC 2 cut(s) 349, 793
BbvI GCAGC 1 cut(s) 508
BccI CCATC 2 cut(s) 221, 457
BciT130I CCWGG 1 cut(s) 536
BcoDI GTCTC 1 cut(s) 693
BfaI CTAG 2 cut(s) 158, 588
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 1 cut(s) 497
BlsI GCNGC 1 cut(s) 498
Bme1390I CCNGG 1 cut(s) 536
Bme18I GGWCC 2 cut(s) 213, 541
BmeT110I CYCGRG 1 cut(s) 491
BmgT120I GGNCC 2 cut(s) 213, 541
BmiI GGNNCC 2 cut(s) 512, 616
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 2 cut(s) 212, 476
BpmI CTGGAG 1 cut(s) 557
BpuEI CTTGAG 2 cut(s) 193, 530
BsaJI CCNNGG 3 cut(s) 140, 237, 737
BsaWI WCCGGW 2 cut(s) 215, 843
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 1 cut(s) 180
Bse1I ACTGG 1 cut(s) 611
Bse21I CCTNAGG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 3 cut(s) 140, 237, 737
BseGI GGATG 4 cut(s) 247, 327, 408, 534
BseLI CCNNNNNNNGG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 1 cut(s) 611
BseXI GCAGC 1 cut(s) 508
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 2 cut(s) 184, 382
BsiHKAI GWGCWC 2 cut(s) 349, 793
BsiHKCI CYCGRG 1 cut(s) 491
BsiSI CCGG 2 cut(s) 216, 844
BslI CCNNNNNNNGG 1 cut(s) 180
BsmAI GTCTC 1 cut(s) 693
BsmI GAATGC 1 cut(s) 826
BsnI GGCC 2 cut(s) 184, 382
BsoBI CYCGRG 1 cut(s) 491
Bsp1286I GDGCHC 2 cut(s) 349, 793
Bsp143I GATC 1 cut(s) 231
BspANI GGCC 2 cut(s) 184, 382
BspCNI CTCAG 1 cut(s) 421
BspLI GGNNCC 2 cut(s) 512, 616
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 1 cut(s) 239
BspQI GCTCTTC 1 cut(s) 439
BsrI ACTGG 1 cut(s) 611
BssECI CCNNGG 3 cut(s) 140, 237, 737
BssMI GATC 1 cut(s) 231
BssT1I CCWWGG 2 cut(s) 237, 737
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 5 cut(s) 164, 554, 562, 625, 783
Bst6I CTCTTC 1 cut(s) 439
BstC8I GCNNGC 4 cut(s) 349, 384, 451, 580
BstDEI CTNAG 3 cut(s) 415, 429, 809
BstF5I GGATG 4 cut(s) 247, 327, 408, 534
BstKTI GATC 1 cut(s) 234
BstMAI GTCTC 1 cut(s) 693
BstMBI GATC 1 cut(s) 231
BstMWI GCNNNNNNNGC 6 cut(s) 36, 154, 473, 496, 502, 588
BstNI CCWGG 1 cut(s) 536
BstSCI CCNGG 1 cut(s) 534
BstV1I GCAGC 1 cut(s) 508
Bsu36I CCTNAGG 1 cut(s) 415
BsuRI GGCC 2 cut(s) 184, 382
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 4 cut(s) 247, 327, 408, 534
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 4 cut(s) 349, 384, 451, 580
Cfr13I GGNCC 2 cut(s) 213, 541
Csp6I GTAC 2 cut(s) 80, 203
CviAII CATG 3 cut(s) 27, 611, 660
CviQI GTAC 2 cut(s) 80, 203
DdeI CTNAG 3 cut(s) 415, 429, 809
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
Eam1104I CTCTTC 1 cut(s) 439
EarI CTCTTC 1 cut(s) 439
Eco130I CCWWGG 2 cut(s) 237, 737
Eco147I AGGCCT 1 cut(s) 382
Eco32I GATATC 1 cut(s) 713
Eco47I GGWCC 2 cut(s) 213, 541
Eco81I CCTNAGG 1 cut(s) 415
Eco88I CYCGRG 1 cut(s) 491
EcoO109I RGGNCCY 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 534
EcoRV GATATC 1 cut(s) 713
EcoT14I CCWWGG 2 cut(s) 237, 737
ErhI CCWWGG 2 cut(s) 237, 737
FaeI CATG 3 cut(s) 30, 614, 663
FalI AAGNNNNNCTT 2 cut(s) 567, 599
FatI CATG 3 cut(s) 26, 610, 659
FauNDI CATATG 1 cut(s) 730
Fnu4HI GCNGC 1 cut(s) 497
FokI GGATG 4 cut(s) 254, 314, 415, 541
Fsp4HI GCNGC 1 cut(s) 497
FspBI CTAG 2 cut(s) 158, 588
GluI GCNGC 1 cut(s) 497
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 2 cut(s) 184, 382
HapII CCGG 2 cut(s) 216, 844
Hin1II CATG 3 cut(s) 30, 614, 663
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 2 cut(s) 410, 775
HpaII CCGG 2 cut(s) 216, 844
HphI GGTGA 3 cut(s) 15, 226, 769
Hpy188I TCNGA 3 cut(s) 144, 370, 636
Hpy188III TCNNGA 2 cut(s) 547, 572
HpyAV CCTTC 2 cut(s) 21, 593
HpyCH4III ACNGT 5 cut(s) 164, 554, 562, 625, 783
HpyCH4V TGCA 6 cut(s) 30, 251, 257, 278, 386, 557
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 154, 473, 496, 502, 588
HpyF3I CTNAG 3 cut(s) 415, 429, 809
Hsp92II CATG 3 cut(s) 30, 614, 663
Kzo9I GATC 1 cut(s) 231
LguI GCTCTTC 1 cut(s) 439
LmnI GCTCC 3 cut(s) 154, 510, 620
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 2 cut(s) 212, 476
MaeI CTAG 2 cut(s) 158, 588
MaeIII GTNAC 3 cut(s) 625, 757, 812
MalI GATC 1 cut(s) 233
MboI GATC 1 cut(s) 231
MboII GAAGA 4 cut(s) 12, 456, 472, 530
MfeI CAATTG 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 349, 793
MluCI AATT 4 cut(s) 116, 126, 309, 653
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 73
MnlI CCTC 8 cut(s) 150, 185, 217, 262, 352, 532, 554, 816
MseI TTAA 2 cut(s) 354, 596
MspI CCGG 2 cut(s) 216, 844
MspR9I CCNGG 1 cut(s) 536
MunI CAATTG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 826
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 6 cut(s) 36, 154, 473, 496, 502, 588
NdeI CATATG 1 cut(s) 730
NdeII GATC 1 cut(s) 231
NlaIII CATG 3 cut(s) 30, 614, 663
NlaIV GGNNCC 2 cut(s) 512, 616
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 1 cut(s) 757
PceI AGGCCT 1 cut(s) 382
PciSI GCTCTTC 1 cut(s) 439
PctI GAATGC 1 cut(s) 826
PfeI GAWTC 1 cut(s) 775
PkrI GCNGC 1 cut(s) 498
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PpuMI RGGWCCY 1 cut(s) 541
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 2 cut(s) 512, 616
PspPI GGNCC 2 cut(s) 213, 541
PspPPI RGGWCCY 1 cut(s) 541
RsaI GTAC 2 cut(s) 81, 204
RsaNI GTAC 2 cut(s) 80, 203
SapI GCTCTTC 1 cut(s) 439
SaqAI TTAA 2 cut(s) 354, 596
SatI GCNGC 1 cut(s) 497
Sau3AI GATC 1 cut(s) 231
Sau96I GGNCC 2 cut(s) 213, 541
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 536
SduI GDGCHC 2 cut(s) 349, 793
SfaNI GCATC 2 cut(s) 212, 476
SinI GGWCC 2 cut(s) 213, 541
SmlI CTYRAG 2 cut(s) 208, 545
SmoI CTYRAG 2 cut(s) 208, 545
Sse9I AATT 4 cut(s) 116, 126, 309, 653
SseBI AGGCCT 1 cut(s) 382
SspMI CTAG 2 cut(s) 158, 588
StuI AGGCCT 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 534
StyI CCWWGG 2 cut(s) 237, 737
TaaI ACNGT 5 cut(s) 164, 554, 562, 625, 783
TaqI TCGA 1 cut(s) 339
TasI AATT 4 cut(s) 116, 126, 309, 653
TfiI GAWTC 1 cut(s) 775
Tru1I TTAA 2 cut(s) 354, 596
Tru9I TTAA 2 cut(s) 354, 596
TseFI GTSAC 1 cut(s) 757
TseI GCWGC 1 cut(s) 496
Tsp45I GTSAC 1 cut(s) 757
TspDTI ATGAA 3 cut(s) 473, 545, 684
VpaK11BI GGWCC 2 cut(s) 213, 541
XapI RAATTY 2 cut(s) 116, 309
XspI CTAG 2 cut(s) 158, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.