Prupe.6G179800_v2.0.a1

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0015333
Physical Location & Seq
Forward (+)
18506359 .. 18507997
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G179800.1

Sequence Viewer

Length: 837 bp
ATGGATGCTTCTACTTCATCATCCAAAGAAGGCACACCAGAGATTGTGTTCTTTGACATAGAAACAAATGTACCCAATAGAGCTGGACAGAGGTTCTGGGTATTGGAATTTGGTGCAATTGTAGTTTGCCCTCAGAAGCTTGTGGAGCTGGAAAGCTATAGCACCCTCATCAGGCCAGGGGACTTGTCTGCTGTGGCATTGAGATCTGGCCGGTCCGACGGGATCACTAGGGAAACTGTTGCAAATGCACCCTTGTTCAAGGAAGTTTCTGATAAGATATTCAGCATTTTGAATGGTAGGGTGTGGGCAGGCCACAACATCCGAAGATTCGATTGCATTCGGATTAAGGAGGCCTTTGCGGAGATTGGCAGGCCTGCTCCTACTCCGGTTGGGATCATTGATTCTTTAGGGGTGTTAACTGACAAGTTTGGCAGAAGAGCTGGTAATATGAAGATGGCAACTTTGGCATCTTACTTCAAGCTTGGGCAGCAAAAGCACAGGAGCCTTGAAGACGTTAGGATGAATTTGGAGGTCCTCAAGAACTGCGCAACTGTGCTTTTTTTGGAAGCAAGCCTCCCTAGCCTGCTAAATGGCAACTGCCGCGGCTCTTCAACGATCACAACGCGAAGCAGAAGCAATGGGAAATCGTTATGCAGAGAAGAAACTAACCGGAAGTCTCCACCCACTGCTTTGATTGGATATCAGAGAGCGGTTCCATATGCGTCAAGGGGGAGTTTGGAAAAGATGACAGAGAAGGTAAAGAATCTGCTGTGTAGAGCTCAAGGAAATCAGCCTCTTAACACCATACTGAAGCATTCTCATTCCCTACTCCGGTGA

Protein Analysis

279

Amino Acids

30.71

Weight (kDa)

9.8

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39810
fragaria_vesca FvH4_1g21490
malus_domestica MD15G1329100.v1.1
prunus_persica Prupe.6G179800_v2.0.a1
pyrus_communis pycom01g05230 pycom15g29710 pycom15g29720
rosa_chinensis RchiOBHm_Chr2g0115371
rosa_laevigata RLG00000018180
rosa_roxburghii Rroxscaffold_2G00130860
rosa_rugosa Rorug02G0195900
rosa_samantha Rh2AG254300 Rh2BG264500 Rh2CG259000 Rh2DG262400
rosa_wichuraiana Rw2G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 547
AccBSI CCGCTC 1 cut(s) 710
AccII CGCG 2 cut(s) 603, 625
AciI CCGC 4 cut(s) 359, 601, 603, 710
AclWI GGATC 2 cut(s) 230, 401
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 2 cut(s) 107, 523
AcuI CTGAAG 1 cut(s) 830
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 2 cut(s) 171, 831
AgsI TTSAA 5 cut(s) 259, 292, 478, 509, 612
AjnI CCWGG 1 cut(s) 175
AluBI AGCT 7 cut(s) 83, 139, 148, 156, 440, 481, 779
AluI AGCT 7 cut(s) 83, 139, 148, 156, 440, 481, 779
Alw21I GWGCWC 1 cut(s) 781
Alw26I GTCTC 1 cut(s) 681
AlwI GGATC 2 cut(s) 230, 401
AoxI GGCC 5 cut(s) 173, 208, 310, 351, 371
ApeKI GCWGC 1 cut(s) 487
ApoI RAATTY 2 cut(s) 107, 523
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 2 cut(s) 213, 532
AvaII GGWCC 2 cut(s) 213, 532
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BanII GRGCYC 1 cut(s) 781
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 781
BbvI GCAGC 1 cut(s) 499
BccI CCATC 1 cut(s) 448
BciT130I CCWGG 1 cut(s) 177
BcoDI GTCTC 1 cut(s) 681
BfaI CTAG 2 cut(s) 228, 579
BfmI CTRYAG 1 cut(s) 157
BglII AGATCT 1 cut(s) 203
BisI GCNGC 3 cut(s) 488, 601, 604
BlsI GCNGC 3 cut(s) 489, 602, 605
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 2 cut(s) 213, 532
BmgT120I GGNCC 2 cut(s) 213, 532
BmiI GGNNCC 2 cut(s) 503, 714
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 476
BpiI GAAGAC 1 cut(s) 516
BpuEI CTTGAG 2 cut(s) 521, 765
BsaJI CCNNGG 2 cut(s) 176, 601
BsaWI WCCGGW 3 cut(s) 385, 669, 831
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 831
Bse118I RCCGGY 1 cut(s) 210
Bse3DI GCAATG 1 cut(s) 643
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 2 cut(s) 176, 601
BseGI GGATG 4 cut(s) 10, 20, 318, 525
BseLI CCNNNNNNNGG 2 cut(s) 171, 831
BseMI GCAATG 1 cut(s) 643
BseMII CTCAG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 499
Bsh1236I CGCG 2 cut(s) 603, 625
BshFI GGCC 5 cut(s) 175, 210, 312, 353, 373
BsiHKAI GWGCWC 1 cut(s) 781
BsiSI CCGG 4 cut(s) 211, 386, 670, 832
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 2 cut(s) 171, 831
BsmAI GTCTC 1 cut(s) 681
BsmFI GGGAC 1 cut(s) 194
BsmI GAATGC 2 cut(s) 336, 814
BsnI GGCC 5 cut(s) 175, 210, 312, 353, 373
Bsp1286I GDGCHC 1 cut(s) 781
Bsp143I GATC 4 cut(s) 203, 222, 393, 615
BspACI CCGC 4 cut(s) 359, 601, 603, 710
BspANI GGCC 5 cut(s) 175, 210, 312, 353, 373
BspCNI CTCAG 1 cut(s) 145
BspFNI CGCG 2 cut(s) 603, 625
BspLI GGNNCC 2 cut(s) 503, 714
BspPI GGATC 2 cut(s) 230, 401
BspQI GCTCTTC 2 cut(s) 430, 613
BsrBI CCGCTC 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 643
BsrFI RCCGGY 1 cut(s) 210
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 2 cut(s) 176, 601
BssMI GATC 4 cut(s) 203, 222, 393, 615
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 2 cut(s) 238, 553
Bst6I CTCTTC 2 cut(s) 430, 613
BstC8I GCNNGC 5 cut(s) 310, 371, 375, 571, 584
BstDEI CTNAG 1 cut(s) 132
BstDSI CCRYGG 1 cut(s) 601
BstF5I GGATG 4 cut(s) 10, 20, 318, 525
BstFNI CGCG 2 cut(s) 603, 625
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 4 cut(s) 206, 225, 396, 618
BstMAI GTCTC 1 cut(s) 681
BstMBI GATC 4 cut(s) 203, 222, 393, 615
BstMWI GCNNNNNNNGC 6 cut(s) 145, 464, 487, 493, 579, 600
BstNI CCWGG 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 175
BstSFI CTRYAG 1 cut(s) 157
BstUI CGCG 2 cut(s) 603, 625
BstV1I GCAGC 1 cut(s) 499
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 1 cut(s) 203
BstYI RGATCY 1 cut(s) 203
BsuRI GGCC 5 cut(s) 175, 210, 312, 353, 373
BtgI CCRYGG 1 cut(s) 601
BtsCI GGATG 4 cut(s) 10, 20, 318, 525
BtsI GCAGTG 1 cut(s) 684
BtsIMutI CAGTG 1 cut(s) 684
Cac8I GCNNGC 5 cut(s) 310, 371, 375, 571, 584
CfoI GCGC 1 cut(s) 548
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 213, 532
Cfr42I CCGCGG 1 cut(s) 604
CpoI CGGWCCG 1 cut(s) 213
CseI GACGC 1 cut(s) 711
Csp6I GTAC 1 cut(s) 71
CspI CGGWCCG 1 cut(s) 213
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 1 cut(s) 132
DpnI GATC 4 cut(s) 205, 224, 395, 617
DpnII GATC 4 cut(s) 203, 222, 393, 615
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 2 cut(s) 430, 613
EarI CTCTTC 2 cut(s) 430, 613
Ecl136II GAGCTC 1 cut(s) 779
Eco147I AGGCCT 2 cut(s) 353, 373
Eco24I GRGCYC 1 cut(s) 781
Eco32I GATATC 1 cut(s) 701
Eco47I GGWCC 2 cut(s) 213, 532
Eco53kI GAGCTC 1 cut(s) 779
Eco57I CTGAAG 1 cut(s) 830
EcoICRI GAGCTC 1 cut(s) 779
EcoO109I RGGNCCY 1 cut(s) 532
EcoRII CCWGG 1 cut(s) 175
EcoRV GATATC 1 cut(s) 701
EcoT38I GRGCYC 1 cut(s) 781
FaiI YATR 7 cut(s) 59, 159, 449, 652, 718, 720, 806
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 1 cut(s) 194
FauNDI CATATG 1 cut(s) 718
Fnu4HI GCNGC 3 cut(s) 488, 601, 604
FokI GGATG 4 cut(s) 7, 17, 305, 532
FriOI GRGCYC 1 cut(s) 781
Fsp4HI GCNGC 3 cut(s) 488, 601, 604
FspBI CTAG 2 cut(s) 228, 579
FspI TGCGCA 1 cut(s) 547
GlaI GCGC 1 cut(s) 547
GluI GCNGC 3 cut(s) 488, 601, 604
HaeIII GGCC 5 cut(s) 175, 210, 312, 353, 373
HapII CCGG 4 cut(s) 211, 386, 670, 832
HgaI GACGC 1 cut(s) 711
HhaI GCGC 1 cut(s) 548
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HindIII AAGCTT 2 cut(s) 137, 479
HinfI GANTC 3 cut(s) 327, 401, 763
HpaI GTTAAC 1 cut(s) 417
HpaII CCGG 4 cut(s) 211, 386, 670, 832
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 6 cut(s) 135, 217, 271, 323, 342, 705
Hpy188III TCNNGA 1 cut(s) 538
Hpy8I GTNNAC 1 cut(s) 417
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 2 cut(s) 23, 748
HpyCH4III ACNGT 2 cut(s) 238, 553
HpyCH4IV ACGT 1 cut(s) 513
HpyCH4V TGCA 5 cut(s) 116, 242, 248, 336, 654
HpyF10VI GCNNNNNNNGC 6 cut(s) 145, 464, 487, 493, 579, 600
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 1 cut(s) 513
HspAI GCGC 1 cut(s) 546
KspAI GTTAAC 1 cut(s) 417
KspI CCGCGG 1 cut(s) 604
Kzo9I GATC 4 cut(s) 203, 222, 393, 615
LguI GCTCTTC 2 cut(s) 430, 613
LmnI GCTCC 3 cut(s) 145, 382, 501
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 1 cut(s) 476
MaeI CTAG 2 cut(s) 228, 579
MaeII ACGT 1 cut(s) 513
MalI GATC 4 cut(s) 205, 224, 395, 617
MbiI CCGCTC 1 cut(s) 710
MboI GATC 4 cut(s) 203, 222, 393, 615
MboII GAAGA 6 cut(s) 336, 447, 463, 521, 600, 671
MfeI CAATTG 1 cut(s) 117
MflI RGATCY 1 cut(s) 203
MhlI GDGCHC 1 cut(s) 781
MluCI AATT 3 cut(s) 107, 117, 523
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 8 cut(s) 84, 141, 176, 343, 523, 545, 584, 804
MseI TTAA 3 cut(s) 345, 416, 798
MspA1I CMGCKG 1 cut(s) 603
MspI CCGG 4 cut(s) 211, 386, 670, 832
MspR9I CCNGG 1 cut(s) 177
MunI CAATTG 1 cut(s) 117
Mva1269I GAATGC 2 cut(s) 336, 814
MvaI CCWGG 1 cut(s) 177
MvnI CGCG 2 cut(s) 603, 625
MwoI GCNNNNNNNGC 6 cut(s) 145, 464, 487, 493, 579, 600
NdeI CATATG 1 cut(s) 718
NdeII GATC 4 cut(s) 203, 222, 393, 615
NlaIV GGNNCC 2 cut(s) 503, 714
NsbI TGCGCA 1 cut(s) 547
PceI AGGCCT 2 cut(s) 353, 373
PciSI GCTCTTC 2 cut(s) 430, 613
PctI GAATGC 2 cut(s) 336, 814
PfeI GAWTC 3 cut(s) 327, 401, 763
PkrI GCNGC 3 cut(s) 489, 602, 605
PpuMI RGGWCCY 1 cut(s) 532
Psp124BI GAGCTC 1 cut(s) 781
Psp5II RGGWCCY 1 cut(s) 532
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspN4I GGNNCC 2 cut(s) 503, 714
PspPI GGNCC 2 cut(s) 213, 532
PspPPI RGGWCCY 1 cut(s) 532
PsuI RGATCY 1 cut(s) 203
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
Rsr2I CGGWCCG 1 cut(s) 213
RsrII CGGWCCG 1 cut(s) 213
SacI GAGCTC 1 cut(s) 781
SacII CCGCGG 1 cut(s) 604
SapI GCTCTTC 2 cut(s) 430, 613
SaqAI TTAA 3 cut(s) 345, 416, 798
SatI GCNGC 3 cut(s) 488, 601, 604
Sau3AI GATC 4 cut(s) 203, 222, 393, 615
Sau96I GGNCC 2 cut(s) 213, 532
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 1 cut(s) 781
SfaNI GCATC 1 cut(s) 476
SfcI CTRYAG 1 cut(s) 157
Sfr303I CCGCGG 1 cut(s) 604
SgrBI CCGCGG 1 cut(s) 604
SinI GGWCC 2 cut(s) 213, 532
SmlI CTYRAG 2 cut(s) 536, 780
SmoI CTYRAG 2 cut(s) 536, 780
Sse9I AATT 3 cut(s) 107, 117, 523
SseBI AGGCCT 2 cut(s) 353, 373
SsiI CCGC 4 cut(s) 359, 601, 603, 710
SspMI CTAG 2 cut(s) 228, 579
SstI GAGCTC 1 cut(s) 781
StuI AGGCCT 2 cut(s) 353, 373
StyD4I CCNGG 1 cut(s) 175
TaaI ACNGT 2 cut(s) 238, 553
TaiI ACGT 1 cut(s) 516
TaqI TCGA 1 cut(s) 330
TasI AATT 3 cut(s) 107, 117, 523
TauI GCSGC 2 cut(s) 603, 606
TfiI GAWTC 3 cut(s) 327, 401, 763
Tru1I TTAA 3 cut(s) 345, 416, 798
Tru9I TTAA 3 cut(s) 345, 416, 798
TscAI CASTG 1 cut(s) 691
TseI GCWGC 1 cut(s) 487
TspDTI ATGAA 3 cut(s) 6, 464, 536
TspRI CASTG 1 cut(s) 691
VpaK11BI GGWCC 2 cut(s) 213, 532
XapI RAATTY 2 cut(s) 107, 523
XspI CTAG 2 cut(s) 228, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.