MD15G1329100.v1.1

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015333
Physical Location & Seq
Forward (+)
36205603 .. 36207058
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1329100.v1.1.491

Sequence Viewer

Length: 840 bp
ATGGATGCTTCTACGTCATCATCCAAACAAGGCACAACAGAGATTGTGTTCTTTGACATAGAAACAAATGTACCCAATAGAGCCGGACAAAGGTTCTGGGTTTTAGAGTTTGGTGCAATTGTAGTTTGCCCTCAGAAGCTTGTTGAGCTGGAAAGCTATAGCACGCTCATTAGGCCGGGGGACTTGTCTGCTGTGGCATTGAGGTCTGGCCGGTCCGAGGGGATCACACAGGAAACTGTTGTAGATGCACCCTTGTTTGAGGAAGTTGCCGATAAGATATTCAGCATTTTGAATGGCAGGGTGTGGGCAGGCCACAACATTCGAAGATTCGATTGTGTTAGGATTAAGGAGGCCTTTACAGAGATCGGTAGGCCTGCACCTCAGCCAGTTGGGATGATTGACTCTTTAAGTGTGTTAACTGGTAAGTTTGGAAGAAGAGCTGGAAATATGAAGATGGCAACATTGGCATCATACTTCAATCTTGGGCAGCAAAAGCACAGGAGCCTTGAAGACGTAAGGATGAACCTGGAGGTCCTCAAGAACTGTGCAACCGTGCTCTTTTTGGAAGCGAGCCTCCCCGGTGTATTAAATGGAAACTGGCCGGGCTCTTCAACGATCACAACGCGGAGTAGAAGTAGCGGGAAATTGCCATGCAGAGAAGAAACTAGCCGCAAGTCTCCAACAACTACTGCTTCTATTGGTTCTCATAGAGCTGTTCCATATGCCTCAAGGGGGAGCTTGGCAAAGATGACAGAAAGGGTAAAGAATCTGTTGTGTAGAGCCCAAGGAAATCAGCCTCTTAATAACATCCTCAAGCATTCTCATTCCCTACTACGGTGA

Protein Analysis

280

Amino Acids

30.66

Weight (kDa)

9.74

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 16 - 179 1.3e-26 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39810
fragaria_vesca FvH4_1g21490
malus_domestica MD15G1329100.v1.1
prunus_persica Prupe.6G179800_v2.0.a1
pyrus_communis pycom01g05230 pycom15g29710 pycom15g29720
rosa_chinensis RchiOBHm_Chr2g0115371
rosa_laevigata RLG00000018180
rosa_roxburghii Rroxscaffold_2G00130860
rosa_rugosa Rorug02G0195900
rosa_samantha Rh2AG254300 Rh2BG264500 Rh2CG259000 Rh2DG262400
rosa_wichuraiana Rw2G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 625
AciI CCGC 3 cut(s) 625, 639, 670
AclWI GGATC 1 cut(s) 230
AcoI YGGCCR 2 cut(s) 208, 599
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 4 cut(s) 90, 217, 732, 834
AgsI TTSAA 4 cut(s) 292, 478, 509, 612
AjnI CCWGG 1 cut(s) 525
AluBI AGCT 6 cut(s) 139, 148, 156, 440, 713, 738
AluI AGCT 6 cut(s) 139, 148, 156, 440, 713, 738
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 1 cut(s) 681
AlwI GGATC 1 cut(s) 230
AoxI GGCC 6 cut(s) 173, 208, 310, 351, 371, 599
ApeKI GCWGC 1 cut(s) 487
AspS9I GGNCC 2 cut(s) 213, 532
AsuC2I CCSGG 3 cut(s) 177, 579, 603
AsuII TTCGAA 1 cut(s) 322
AvaII GGWCC 2 cut(s) 213, 532
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BanII GRGCYC 2 cut(s) 608, 784
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 558
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 1 cut(s) 499
BccI CCATC 1 cut(s) 448
BciT130I CCWGG 1 cut(s) 527
BcnI CCSGG 3 cut(s) 177, 579, 603
BcoDI GTCTC 1 cut(s) 681
BfaI CTAG 1 cut(s) 666
BfmI CTRYAG 1 cut(s) 157
BisI GCNGC 2 cut(s) 488, 670
BlsI GCNGC 2 cut(s) 489, 671
Bme1390I CCNGG 4 cut(s) 177, 527, 579, 603
Bme18I GGWCC 2 cut(s) 213, 532
BmgT120I GGNCC 2 cut(s) 213, 532
BmiI GGNNCC 1 cut(s) 503
BmrFI CCNGG 4 cut(s) 177, 527, 579, 603
BmsI GCATC 2 cut(s) 235, 476
BpiI GAAGAC 1 cut(s) 516
BpmI CTGGAG 1 cut(s) 548
Bpu10I CCTNAGC 1 cut(s) 381
Bpu14I TTCGAA 1 cut(s) 322
BpuEI CTTGAG 3 cut(s) 521, 712, 797
BpuMI CCSGG 3 cut(s) 177, 579, 603
BsaJI CCNNGG 4 cut(s) 176, 216, 577, 784
BsaXI ACNNNNNCTCC 2 cut(s) 619, 649
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 217, 732, 834
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 3 cut(s) 386, 424, 602
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 4 cut(s) 176, 216, 577, 784
BseGI GGATG 5 cut(s) 10, 20, 399, 525, 807
BseLI CCNNNNNNNGG 4 cut(s) 90, 217, 732, 834
BseMII CTCAG 2 cut(s) 146, 395
BseNI ACTGG 3 cut(s) 386, 424, 602
BseXI GCAGC 1 cut(s) 499
BsgI GTGCAG 1 cut(s) 360
Bsh1236I CGCG 1 cut(s) 625
BshFI GGCC 6 cut(s) 175, 210, 312, 353, 373, 601
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 5 cut(s) 84, 176, 211, 579, 602
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 4 cut(s) 90, 217, 732, 834
BsmAI GTCTC 1 cut(s) 681
BsmFI GGGAC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 6 cut(s) 175, 210, 312, 353, 373, 601
Bsp119I TTCGAA 1 cut(s) 322
Bsp1286I GDGCHC 3 cut(s) 558, 608, 784
Bsp143I GATC 3 cut(s) 222, 363, 615
BspACI CCGC 3 cut(s) 625, 639, 670
BspANI GGCC 6 cut(s) 175, 210, 312, 353, 373, 601
BspCNI CTCAG 2 cut(s) 145, 394
BspFNI CGCG 1 cut(s) 625
BspLI GGNNCC 1 cut(s) 503
BspPI GGATC 1 cut(s) 230
BspQI GCTCTTC 2 cut(s) 430, 613
BspT104I TTCGAA 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 210
BsrI ACTGG 3 cut(s) 386, 424, 602
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 4 cut(s) 176, 216, 577, 784
BssMI GATC 3 cut(s) 222, 363, 615
BssT1I CCWWGG 1 cut(s) 784
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 4 cut(s) 238, 545, 553, 837
Bst6I CTCTTC 2 cut(s) 430, 613
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 4 cut(s) 164, 310, 375, 571
BstDEI CTNAG 2 cut(s) 132, 381
BstF5I GGATG 5 cut(s) 10, 20, 399, 525, 807
BstFNI CGCG 1 cut(s) 625
BstKTI GATC 3 cut(s) 225, 366, 618
BstMAI GTCTC 1 cut(s) 681
BstMBI GATC 3 cut(s) 222, 363, 615
BstMWI GCNNNNNNNGC 4 cut(s) 145, 172, 464, 493
BstNI CCWGG 1 cut(s) 527
BstSCI CCNGG 4 cut(s) 175, 525, 577, 601
BstSFI CTRYAG 1 cut(s) 157
BstUI CGCG 1 cut(s) 625
BstV1I GCAGC 1 cut(s) 499
BstV2I GAAGAC 1 cut(s) 516
BsuRI GGCC 6 cut(s) 175, 210, 312, 353, 373, 601
BtsCI GGATG 5 cut(s) 10, 20, 399, 525, 807
Cac8I GCNNGC 4 cut(s) 164, 310, 375, 571
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 213, 532
CpoI CGGWCCG 1 cut(s) 213
Csp6I GTAC 1 cut(s) 71
CspI CGGWCCG 1 cut(s) 213
CviAII CATG 1 cut(s) 651
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 2 cut(s) 132, 381
DpnI GATC 3 cut(s) 224, 365, 617
DpnII GATC 3 cut(s) 222, 363, 615
EaeI YGGCCR 2 cut(s) 208, 599
Eam1104I CTCTTC 2 cut(s) 430, 613
EarI CTCTTC 2 cut(s) 430, 613
Eco130I CCWWGG 1 cut(s) 784
Eco147I AGGCCT 2 cut(s) 353, 373
Eco24I GRGCYC 2 cut(s) 608, 784
Eco47I GGWCC 2 cut(s) 213, 532
EcoO109I RGGNCCY 1 cut(s) 532
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 784
EcoT38I GRGCYC 2 cut(s) 608, 784
ErhI CCWWGG 1 cut(s) 784
FaeI CATG 1 cut(s) 654
FaiI YATR 8 cut(s) 59, 159, 449, 472, 652, 708, 721, 723
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 1 cut(s) 194
FatI CATG 1 cut(s) 650
FauI CCCGC 1 cut(s) 632
FauNDI CATATG 1 cut(s) 721
Fnu4HI GCNGC 2 cut(s) 488, 670
FokI GGATG 5 cut(s) 7, 17, 406, 532, 794
FriOI GRGCYC 2 cut(s) 608, 784
Fsp4HI GCNGC 2 cut(s) 488, 670
FspBI CTAG 1 cut(s) 666
GluI GCNGC 2 cut(s) 488, 670
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 6 cut(s) 175, 210, 312, 353, 373, 601
HapII CCGG 5 cut(s) 84, 176, 211, 579, 602
Hin1II CATG 1 cut(s) 654
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 3 cut(s) 327, 401, 766
HpaI GTTAAC 1 cut(s) 417
HpaII CCGG 5 cut(s) 84, 176, 211, 579, 602
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 2 cut(s) 135, 217
Hpy188III TCNNGA 1 cut(s) 538
Hpy8I GTNNAC 1 cut(s) 417
HpyCH4III ACNGT 4 cut(s) 238, 545, 553, 837
HpyCH4IV ACGT 2 cut(s) 14, 513
HpyCH4V TGCA 5 cut(s) 116, 248, 377, 548, 654
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 172, 464, 493
HpyF3I CTNAG 2 cut(s) 132, 381
HpySE526I ACGT 2 cut(s) 14, 513
Hsp92II CATG 1 cut(s) 654
KspAI GTTAAC 1 cut(s) 417
Kzo9I GATC 3 cut(s) 222, 363, 615
LguI GCTCTTC 2 cut(s) 430, 613
LmnI GCTCC 2 cut(s) 501, 735
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 2 cut(s) 235, 476
MaeI CTAG 1 cut(s) 666
MaeII ACGT 2 cut(s) 14, 513
MalI GATC 3 cut(s) 224, 365, 617
MboI GATC 3 cut(s) 222, 363, 615
MboII GAAGA 7 cut(s) 336, 444, 447, 463, 521, 600, 671
MfeI CAATTG 1 cut(s) 117
MhlI GDGCHC 3 cut(s) 558, 608, 784
MluCI AATT 2 cut(s) 117, 644
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 1 cut(s) 704
MseI TTAA 5 cut(s) 345, 407, 416, 587, 801
MspI CCGG 5 cut(s) 84, 176, 211, 579, 602
MspR9I CCNGG 4 cut(s) 177, 527, 579, 603
MunI CAATTG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 817
MvaI CCWGG 1 cut(s) 527
MvnI CGCG 1 cut(s) 625
MwoI GCNNNNNNNGC 4 cut(s) 145, 172, 464, 493
NciI CCSGG 3 cut(s) 177, 579, 603
NdeI CATATG 1 cut(s) 721
NdeII GATC 3 cut(s) 222, 363, 615
NlaIII CATG 1 cut(s) 654
NlaIV GGNNCC 1 cut(s) 503
NspV TTCGAA 1 cut(s) 322
PceI AGGCCT 2 cut(s) 353, 373
PciSI GCTCTTC 2 cut(s) 430, 613
PctI GAATGC 1 cut(s) 817
PfeI GAWTC 2 cut(s) 327, 766
PkrI GCNGC 2 cut(s) 489, 671
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
PpuMI RGGWCCY 1 cut(s) 532
Psp5II RGGWCCY 1 cut(s) 532
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 1 cut(s) 503
PspPI GGNCC 2 cut(s) 213, 532
PspPPI RGGWCCY 1 cut(s) 532
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
Rsr2I CGGWCCG 1 cut(s) 213
RsrII CGGWCCG 1 cut(s) 213
SapI GCTCTTC 2 cut(s) 430, 613
SaqAI TTAA 5 cut(s) 345, 407, 416, 587, 801
SatI GCNGC 2 cut(s) 488, 670
Sau3AI GATC 3 cut(s) 222, 363, 615
Sau96I GGNCC 2 cut(s) 213, 532
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 4 cut(s) 177, 527, 579, 603
SduI GDGCHC 3 cut(s) 558, 608, 784
SfaNI GCATC 2 cut(s) 235, 476
SfcI CTRYAG 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 322
SinI GGWCC 2 cut(s) 213, 532
SmlI CTYRAG 3 cut(s) 536, 727, 812
SmoI CTYRAG 3 cut(s) 536, 727, 812
Sse9I AATT 2 cut(s) 117, 644
SseBI AGGCCT 2 cut(s) 353, 373
SsiI CCGC 3 cut(s) 625, 639, 670
SspMI CTAG 1 cut(s) 666
StuI AGGCCT 2 cut(s) 353, 373
StyD4I CCNGG 4 cut(s) 175, 525, 577, 601
StyI CCWWGG 1 cut(s) 784
TaaI ACNGT 4 cut(s) 238, 545, 553, 837
TaiI ACGT 2 cut(s) 17, 516
TaqI TCGA 2 cut(s) 322, 330
TasI AATT 2 cut(s) 117, 644
TauI GCSGC 1 cut(s) 672
TfiI GAWTC 2 cut(s) 327, 766
Tru1I TTAA 5 cut(s) 345, 407, 416, 587, 801
Tru9I TTAA 5 cut(s) 345, 407, 416, 587, 801
TseI GCWGC 1 cut(s) 487
TspDTI ATGAA 2 cut(s) 464, 536
VpaK11BI GGWCC 2 cut(s) 213, 532
XspI CTAG 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.