RLG00000018180

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015333
Physical Location & Seq
Reverse (-)
26499286 .. 26500616
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018180

Sequence Viewer

Length: 852 bp
ATGGACTACCCTTCTACATCTTCATCATGCAAAGAAAGCACATCAACAGAGATTGTGTTCTTTGACATAGAAACAAATGTACCCAATAGAGTTGGACAAAGGTTCTGGGTATTAGAATTTGGGGCAATTGTTGTTTGCCCTCGAAAGCTTGTCGAGCTAGACAGCTATAGCAGCCTCATTAGGCCAGCAGACTTGTCTGCAGTACCCTCAAGGTCCGGTCGATGTGACGGGATCACTAAGGATGTTGTTGCAAATGCACCTACGTTTGAGCAAGTTGCTGATAAGATATTCAGCATTTTGAATGGCAGAATTTGGGCAGGTCATAACATCCAGCGGTTTGATTGTGCTCGCATTAAGGAGGCGTTTTCGGACATTGGTAGGCCTGCGCCCACCCCTGTTGGGATGATAGACTCTCTAGGGGTACTAACTGAGAAATTTGGCAGAAGAGCTGGCAATATGAAGATGGCATCATTGGCAACTTACTTCAAGCTTGGGCAGCAAAAGCACCGGAGCCTTGAAGATGTTAGGATGAACCTGGAGGTCCTCAAGAACTGTGCAACTGTGCTGTTTCTGGAAGCAACCCTTCCTAGCGTGTTAGATGGCAACTGGCGTGGCTCTTCAACAGTTACGACTCGGAGTAGAAGTAATGGGAAATTGCCATGCCGAGATGAAACAACAAGCCGGAAGTCTCCCCCAACAACTGCTTTTATCGGATATCAAAGAGCAGTTCCATATACTCCAAGGGGGAGTTTGGGAAAGGTGACAGAAGGGGTAAAGAATCTATTGTGTAGAGCACAAGGAAACCAGCCTCTTAGTAACATATTGAAGCATTCTCATTCACTACTCCGGTGA

Protein Analysis

284

Amino Acids

31.21

Weight (kDa)

9.57

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 19 - 182 6.6e-27 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39810
fragaria_vesca FvH4_1g21490
malus_domestica MD15G1329100.v1.1
prunus_persica Prupe.6G179800_v2.0.a1
pyrus_communis pycom01g05230 pycom15g29710 pycom15g29720
rosa_chinensis RchiOBHm_Chr2g0115371
rosa_laevigata RLG00000018180
rosa_roxburghii Rroxscaffold_2G00130860
rosa_rugosa Rorug02G0195900
rosa_samantha Rh2AG254300 Rh2BG264500 Rh2CG259000 Rh2DG262400
rosa_wichuraiana Rw2G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
AciI CCGC 1 cut(s) 334
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 3 cut(s) 116, 309, 434
AfaI GTAC 3 cut(s) 81, 204, 423
AfiI CCNNNNNNNGG 1 cut(s) 399
AgsI TTSAA 5 cut(s) 301, 487, 518, 621, 826
AjnI CCWGG 1 cut(s) 534
AluBI AGCT 5 cut(s) 148, 157, 165, 449, 490
AluI AGCT 5 cut(s) 148, 157, 165, 449, 490
Alw21I GWGCWC 2 cut(s) 349, 796
Alw26I GTCTC 1 cut(s) 693
AlwI GGATC 1 cut(s) 239
AoxI GGCC 2 cut(s) 182, 380
ApeKI GCWGC 2 cut(s) 171, 496
ApoI RAATTY 3 cut(s) 116, 309, 434
AspLEI GCGC 1 cut(s) 388
AspS9I GGNCC 2 cut(s) 213, 541
AsuHPI GGTGA 1 cut(s) 772
AvaII GGWCC 2 cut(s) 213, 541
BaeI ACNNNNGTAYC 2 cut(s) 63, 96
Bbv12I GWGCWC 2 cut(s) 349, 796
BbvI GCAGC 2 cut(s) 183, 508
BccI CCATC 2 cut(s) 457, 593
BciT130I CCWGG 1 cut(s) 536
BcoDI GTCTC 1 cut(s) 693
BfaI CTAG 3 cut(s) 158, 416, 588
BfmI CTRYAG 2 cut(s) 166, 198
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 2 cut(s) 172, 497
BlsI GCNGC 2 cut(s) 173, 498
Bme1390I CCNGG 1 cut(s) 536
Bme18I GGWCC 2 cut(s) 213, 541
BmgT120I GGNCC 2 cut(s) 213, 541
BmiI GGNNCC 1 cut(s) 512
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 1 cut(s) 476
BpmI CTGGAG 1 cut(s) 557
BpuEI CTTGAG 2 cut(s) 193, 530
BsaJI CCNNGG 1 cut(s) 740
BsaWI WCCGGW 3 cut(s) 215, 507, 846
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 611
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 1 cut(s) 740
BseGI GGATG 4 cut(s) 247, 327, 408, 534
BseLI CCNNNNNNNGG 1 cut(s) 399
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 1 cut(s) 611
BseXI GCAGC 2 cut(s) 183, 508
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 2 cut(s) 184, 382
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 2 cut(s) 349, 796
BsiSI CCGG 4 cut(s) 216, 508, 682, 847
BslI CCNNNNNNNGG 1 cut(s) 399
BsmAI GTCTC 1 cut(s) 693
BsmI GAATGC 1 cut(s) 829
BsnI GGCC 2 cut(s) 184, 382
Bsp1286I GDGCHC 2 cut(s) 349, 796
Bsp143I GATC 1 cut(s) 231
BspACI CCGC 1 cut(s) 334
BspANI GGCC 2 cut(s) 184, 382
BspCNI CTCAG 1 cut(s) 421
BspLI GGNNCC 1 cut(s) 512
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 1 cut(s) 239
BspQI GCTCTTC 2 cut(s) 439, 622
BsrI ACTGG 1 cut(s) 611
BssECI CCNNGG 1 cut(s) 740
BssMI GATC 1 cut(s) 231
BssT1I CCWWGG 1 cut(s) 740
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 3 cut(s) 554, 562, 625
Bst6I CTCTTC 2 cut(s) 439, 622
BstC8I GCNNGC 4 cut(s) 186, 349, 384, 451
BstDEI CTNAG 3 cut(s) 237, 429, 812
BstF5I GGATG 4 cut(s) 247, 327, 408, 534
BstHHI GCGC 1 cut(s) 388
BstKTI GATC 1 cut(s) 234
BstMAI GTCTC 1 cut(s) 693
BstMBI GATC 1 cut(s) 231
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 6 cut(s) 36, 154, 171, 473, 496, 502
BstNI CCWGG 1 cut(s) 536
BstSCI CCNGG 1 cut(s) 534
BstSFI CTRYAG 2 cut(s) 166, 198
BstV1I GCAGC 2 cut(s) 183, 508
BsuRI GGCC 2 cut(s) 184, 382
BtsCI GGATG 4 cut(s) 247, 327, 408, 534
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 4 cut(s) 186, 349, 384, 451
CfoI GCGC 1 cut(s) 388
Cfr13I GGNCC 2 cut(s) 213, 541
Csp6I GTAC 3 cut(s) 80, 203, 422
CspCI CAANNNNNGTGG 4 cut(s) 379, 414, 592, 627
CviAII CATG 2 cut(s) 27, 660
CviQI GTAC 3 cut(s) 80, 203, 422
DdeI CTNAG 3 cut(s) 237, 429, 812
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 439, 622
EarI CTCTTC 2 cut(s) 439, 622
Eco130I CCWWGG 1 cut(s) 740
Eco147I AGGCCT 1 cut(s) 382
Eco32I GATATC 1 cut(s) 716
Eco47I GGWCC 2 cut(s) 213, 541
EcoO109I RGGNCCY 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 534
EcoRV GATATC 1 cut(s) 716
EcoT14I CCWWGG 1 cut(s) 740
ErhI CCWWGG 1 cut(s) 740
FaeI CATG 2 cut(s) 30, 663
FaiI YATR 9 cut(s) 28, 68, 168, 324, 458, 661, 733, 735, 821
FalI AAGNNNNNCTT 2 cut(s) 567, 599
FatI CATG 2 cut(s) 26, 659
Fnu4HI GCNGC 2 cut(s) 172, 497
FokI GGATG 4 cut(s) 254, 314, 415, 541
Fsp4HI GCNGC 2 cut(s) 172, 497
FspBI CTAG 3 cut(s) 158, 416, 588
GlaI GCGC 1 cut(s) 387
GluI GCNGC 2 cut(s) 172, 497
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 2 cut(s) 184, 382
HapII CCGG 4 cut(s) 216, 508, 682, 847
HhaI GCGC 1 cut(s) 388
Hin1II CATG 2 cut(s) 30, 663
Hin6I GCGC 1 cut(s) 386
HinP1I GCGC 1 cut(s) 386
HindIII AAGCTT 2 cut(s) 146, 488
HinfI GANTC 3 cut(s) 410, 631, 778
HpaII CCGG 4 cut(s) 216, 508, 682, 847
HphI GGTGA 1 cut(s) 772
Hpy188I TCNGA 3 cut(s) 370, 636, 713
Hpy188III TCNNGA 2 cut(s) 547, 572
HpyAV CCTTC 3 cut(s) 21, 593, 761
HpyCH4III ACNGT 3 cut(s) 554, 562, 625
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 5 cut(s) 30, 200, 251, 257, 557
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 154, 171, 473, 496, 502
HpyF3I CTNAG 3 cut(s) 237, 429, 812
HpySE526I ACGT 1 cut(s) 263
Hsp92II CATG 2 cut(s) 30, 663
HspAI GCGC 1 cut(s) 386
Kzo9I GATC 1 cut(s) 231
LguI GCTCTTC 2 cut(s) 439, 622
LmnI GCTCC 1 cut(s) 510
Lsp1109I GCAGC 2 cut(s) 183, 508
LweI GCATC 1 cut(s) 476
MaeI CTAG 3 cut(s) 158, 416, 588
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 4 cut(s) 224, 625, 760, 815
MalI GATC 1 cut(s) 233
MboI GATC 1 cut(s) 231
MboII GAAGA 5 cut(s) 12, 456, 472, 530, 609
MfeI CAATTG 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 349, 796
MluCI AATT 5 cut(s) 116, 126, 309, 434, 653
MlyI GAGTC 2 cut(s) 404, 625
MmeI TCCRAC 1 cut(s) 73
MnlI CCTC 7 cut(s) 150, 185, 217, 352, 532, 554, 819
MseI TTAA 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 334
MspI CCGG 4 cut(s) 216, 508, 682, 847
MspR9I CCNGG 1 cut(s) 536
MunI CAATTG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 6 cut(s) 36, 154, 171, 473, 496, 502
NdeII GATC 1 cut(s) 231
NlaIII CATG 2 cut(s) 30, 663
NlaIV GGNNCC 1 cut(s) 512
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 2 cut(s) 224, 760
PceI AGGCCT 1 cut(s) 382
PciSI GCTCTTC 2 cut(s) 439, 622
PctI GAATGC 1 cut(s) 829
PfeI GAWTC 1 cut(s) 778
PkrI GCNGC 2 cut(s) 173, 498
PleI GAGTC 2 cut(s) 404, 625
PpsI GAGTC 2 cut(s) 404, 625
PpuMI RGGWCCY 1 cut(s) 541
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 1 cut(s) 512
PspPI GGNCC 2 cut(s) 213, 541
PspPPI RGGWCCY 1 cut(s) 541
PstI CTGCAG 1 cut(s) 202
RsaI GTAC 3 cut(s) 81, 204, 423
RsaNI GTAC 3 cut(s) 80, 203, 422
SapI GCTCTTC 2 cut(s) 439, 622
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 2 cut(s) 172, 497
Sau3AI GATC 1 cut(s) 231
Sau96I GGNCC 2 cut(s) 213, 541
SchI GAGTC 2 cut(s) 404, 625
ScrFI CCNGG 1 cut(s) 536
SduI GDGCHC 2 cut(s) 349, 796
SfaNI GCATC 1 cut(s) 476
SfcI CTRYAG 2 cut(s) 166, 198
SinI GGWCC 2 cut(s) 213, 541
SmlI CTYRAG 2 cut(s) 208, 545
SmoI CTYRAG 2 cut(s) 208, 545
Sse9I AATT 5 cut(s) 116, 126, 309, 434, 653
SseBI AGGCCT 1 cut(s) 382
SsiI CCGC 1 cut(s) 334
SspMI CTAG 3 cut(s) 158, 416, 588
StuI AGGCCT 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 534
StyI CCWWGG 1 cut(s) 740
TaaI ACNGT 3 cut(s) 554, 562, 625
TaiI ACGT 1 cut(s) 266
TaqI TCGA 3 cut(s) 142, 153, 220
TasI AATT 5 cut(s) 116, 126, 309, 434, 653
TfiI GAWTC 1 cut(s) 778
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TseFI GTSAC 2 cut(s) 224, 760
TseI GCWGC 2 cut(s) 171, 496
Tsp45I GTSAC 2 cut(s) 224, 760
TspDTI ATGAA 4 cut(s) 12, 473, 545, 684
VpaK11BI GGWCC 2 cut(s) 213, 541
XapI RAATTY 3 cut(s) 116, 309, 434
XspI CTAG 3 cut(s) 158, 416, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.