pycom01g05230

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0015333
Physical Location & Seq
Forward (+)
4868949 .. 4869422
474 bp
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UTR
Exon/CDS
Intron
pycom01g05230.1

Sequence Viewer

Length: 474 bp
ATGGATGCTTCAACTTCATCATCCAAACAAGGCACAACAGAGATTGTGTTCTTTGACATAGAAACAAATGTACCCAATAAAGCTGGACAAAGGTTCTGGGTTTTAGAATTTGGTGCAATTTTGGTTTGCCCTCAGAACCTTGTTGAGCTGGAAACCTATAGCACACTCATTAGGCCGGGGGACTTGTCTGTTGTGGCATTGAGGTCTGGCCGGTCTGACGGGATCACTCAGGAAACTGTTGCAAAAGCACCCTTGTTTGAGGAAGTTGCTGATAAGATATTCAGCATTTTGAATGGAAGGGTGTGGGCAGGCCACAACATTCGAAGATTCGATTGCGTTCGGATTAAGGAGGCTTTTGCAGAGATCGGTAGGCCTGCACCTACGCCGGTTGGGATGATGGACTCTTTAGGGGTGTTAACTGGTAAATTTGGAAGAAGAGCTGGCAATATGAAGGTAATTAGATATAAATGTTAA

Protein Analysis

158

Amino Acids

17.3

Weight (kDa)

8.88

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 16 - 148 2.7e-18 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39810
fragaria_vesca FvH4_1g21490
malus_domestica MD15G1329100.v1.1
prunus_persica Prupe.6G179800_v2.0.a1
pyrus_communis pycom01g05230 pycom15g29710 pycom15g29720
rosa_chinensis RchiOBHm_Chr2g0115371
rosa_laevigata RLG00000018180
rosa_roxburghii Rroxscaffold_2G00130860
rosa_rugosa Rorug02G0195900
rosa_samantha Rh2AG254300 Rh2BG264500 Rh2CG259000 Rh2DG262400
rosa_wichuraiana Rw2G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 230
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 2 cut(s) 107, 425
AfaI GTAC 1 cut(s) 72
AgsI TTSAA 2 cut(s) 12, 292
AluBI AGCT 3 cut(s) 83, 148, 440
AluI AGCT 3 cut(s) 83, 148, 440
AlwI GGATC 1 cut(s) 230
AoxI GGCC 4 cut(s) 173, 208, 310, 371
ApoI RAATTY 2 cut(s) 107, 425
AsuC2I CCSGG 1 cut(s) 177
AsuII TTCGAA 1 cut(s) 322
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BccI CCATC 1 cut(s) 391
BcnI CCSGG 1 cut(s) 177
BfmI CTRYAG 1 cut(s) 157
Bme1390I CCNGG 1 cut(s) 177
BmrFI CCNGG 1 cut(s) 177
Bpu14I TTCGAA 1 cut(s) 322
BpuMI CCSGG 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 176
Bse118I RCCGGY 2 cut(s) 210, 385
Bse1I ACTGG 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 176
BseGI GGATG 3 cut(s) 10, 20, 399
BseMII CTCAG 2 cut(s) 146, 242
BseNI ACTGG 1 cut(s) 424
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 4 cut(s) 175, 210, 312, 373
BsiSI CCGG 3 cut(s) 176, 211, 386
BslFI GGGAC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 4 cut(s) 175, 210, 312, 373
Bsp119I TTCGAA 1 cut(s) 322
Bsp143I GATC 2 cut(s) 222, 363
BspANI GGCC 4 cut(s) 175, 210, 312, 373
BspCNI CTCAG 2 cut(s) 145, 241
BspPI GGATC 1 cut(s) 230
BspQI GCTCTTC 1 cut(s) 430
BspT104I TTCGAA 1 cut(s) 322
BsrFI RCCGGY 2 cut(s) 210, 385
BsrI ACTGG 1 cut(s) 424
BssAI RCCGGY 2 cut(s) 210, 385
BssECI CCNNGG 1 cut(s) 176
BssMI GATC 2 cut(s) 222, 363
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 1 cut(s) 430
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 3 cut(s) 310, 375, 442
BstDEI CTNAG 2 cut(s) 132, 228
BstF5I GGATG 3 cut(s) 10, 20, 399
BstKTI GATC 2 cut(s) 225, 366
BstMBI GATC 2 cut(s) 222, 363
BstSCI CCNGG 1 cut(s) 175
BstSFI CTRYAG 1 cut(s) 157
BsuRI GGCC 4 cut(s) 175, 210, 312, 373
BtsCI GGATG 3 cut(s) 10, 20, 399
Cac8I GCNNGC 3 cut(s) 310, 375, 442
Cfr10I RCCGGY 2 cut(s) 210, 385
Csp6I GTAC 1 cut(s) 71
CviJI RGCY 8 cut(s) 83, 148, 175, 210, 312, 353, 373, 440
CviKI_1 RGCY 8 cut(s) 83, 148, 175, 210, 312, 353, 373, 440
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 2 cut(s) 132, 228
DpnI GATC 2 cut(s) 224, 365
DpnII GATC 2 cut(s) 222, 363
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 430
EarI CTCTTC 1 cut(s) 430
Eco147I AGGCCT 1 cut(s) 373
FaiI YATR 4 cut(s) 59, 159, 449, 465
FaqI GGGAC 1 cut(s) 194
FokI GGATG 3 cut(s) 7, 17, 406
HaeIII GGCC 4 cut(s) 175, 210, 312, 373
HapII CCGG 3 cut(s) 176, 211, 386
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HinfI GANTC 2 cut(s) 327, 401
HpaI GTTAAC 1 cut(s) 417
HpaII CCGG 3 cut(s) 176, 211, 386
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 3 cut(s) 135, 217, 342
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 1 cut(s) 417
HpyAV CCTTC 2 cut(s) 291, 445
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4V TGCA 4 cut(s) 116, 242, 359, 377
HpyF3I CTNAG 2 cut(s) 132, 228
KspAI GTTAAC 1 cut(s) 417
Kzo9I GATC 2 cut(s) 222, 363
LguI GCTCTTC 1 cut(s) 430
MalI GATC 2 cut(s) 224, 365
MboI GATC 2 cut(s) 222, 363
MboII GAAGA 3 cut(s) 336, 444, 447
MluCI AATT 4 cut(s) 107, 117, 425, 456
MlyI GAGTC 1 cut(s) 395
MnlI CCTC 4 cut(s) 141, 195, 253, 343
MseI TTAA 3 cut(s) 345, 416, 472
MspI CCGG 3 cut(s) 176, 211, 386
MspR9I CCNGG 1 cut(s) 177
NciI CCSGG 1 cut(s) 177
NdeII GATC 2 cut(s) 222, 363
NspV TTCGAA 1 cut(s) 322
PceI AGGCCT 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 430
PfeI GAWTC 1 cut(s) 327
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 430
SaqAI TTAA 3 cut(s) 345, 416, 472
Sau3AI GATC 2 cut(s) 222, 363
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 1 cut(s) 177
SetI ASST 9 cut(s) 85, 95, 141, 150, 158, 206, 382, 442, 456
SfcI CTRYAG 1 cut(s) 157
SfuI TTCGAA 1 cut(s) 322
Sse9I AATT 4 cut(s) 107, 117, 425, 456
SseBI AGGCCT 1 cut(s) 373
StuI AGGCCT 1 cut(s) 373
StyD4I CCNGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 238
TaqI TCGA 2 cut(s) 322, 330
TasI AATT 4 cut(s) 107, 117, 425, 456
TfiI GAWTC 1 cut(s) 327
Tru1I TTAA 3 cut(s) 345, 416, 472
Tru9I TTAA 3 cut(s) 345, 416, 472
TspDTI ATGAA 2 cut(s) 6, 464
XapI RAATTY 2 cut(s) 107, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.