pycom15g29710

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015333
Physical Location & Seq
Forward (+)
26663054 .. 26664382
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g29710.3

Sequence Viewer

Length: 732 bp
ATGGATGCTTCTACGTCATCATCCAAACAAGGCACGACAGAGATTGTGTTCTTTGACATAGAAACAAATGTACCCAATAGAGCCGGACAAAGGTTCTGGGTTTTAGAGTTTGGTGCAATTGTAGTTTGCCCTCAGAAGCTTGTTGAGCTGGAAAGTTATAGCACGCTCATTAGGCCGGGGGACTTGTCTGCTGTAGCATTGAGGTCTGGCCGGTCCGAGGGGATCACACAGGAAACTGTTGTAGATGCACCCTTGTTTGAGGAAGTTGCCGATAAGATATTCAGCATTTTGAATGGCAGGGTGTGGGCAGGCCACAACATTCGAAGATTCGATTGTGTTAGGATTAAGGAGGCCTTTGCAGAGATCGGTAGGCCTGCACCTCAGCCAGTTGGGATGATTGATTCTTTAGGTGTGTTAACTGGTAAGTTTGGAAGAAGAGCTGGAAATATGAAGGAAGCGAGCCTCCCCGGTGTATTAAATGGAAACTGGCCTGGCTCTTCAACGATCACAACGCGGAGTAGAAGTAGCGGGAAATTGCCATGCAGAGAAGAAACTAGCCGCAAGTCTCCAACAACTACTGCTTCTATTGGTTCTCATAGAGCTGTTCCATATGCCTCAAGGGGGAGCTTGGCAAAGCAGATGACAGAAAGGGTAAAGAATCTGTTGTGTAGAGCCCAAGGAAATCAGCCTCTTAACAACATCCTCAAGCATTCTCATTCCCTACTACGGTGA

Protein Analysis

244

Amino Acids

26.49

Weight (kDa)

9.85

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39810
fragaria_vesca FvH4_1g21490
malus_domestica MD15G1329100.v1.1
prunus_persica Prupe.6G179800_v2.0.a1
pyrus_communis pycom01g05230 pycom15g29710 pycom15g29720
rosa_chinensis RchiOBHm_Chr2g0115371
rosa_laevigata RLG00000018180
rosa_roxburghii Rroxscaffold_2G00130860
rosa_rugosa Rorug02G0195900
rosa_samantha Rh2AG254300 Rh2BG264500 Rh2CG259000 Rh2DG262400
rosa_wichuraiana Rw2G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 514
AciI CCGC 3 cut(s) 514, 528, 559
AclWI GGATC 1 cut(s) 230
AcoI YGGCCR 1 cut(s) 208
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 4 cut(s) 90, 217, 621, 726
AgsI TTSAA 2 cut(s) 292, 501
AjnI CCWGG 1 cut(s) 490
AluBI AGCT 5 cut(s) 139, 148, 440, 602, 627
AluI AGCT 5 cut(s) 139, 148, 440, 602, 627
Alw26I GTCTC 1 cut(s) 570
AlwI GGATC 1 cut(s) 230
AoxI GGCC 6 cut(s) 173, 208, 310, 351, 371, 488
AspS9I GGNCC 1 cut(s) 213
AsuC2I CCSGG 2 cut(s) 177, 468
AsuII TTCGAA 1 cut(s) 322
AvaII GGWCC 1 cut(s) 213
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BanII GRGCYC 1 cut(s) 676
BbvCI CCTCAGC 1 cut(s) 381
BciT130I CCWGG 1 cut(s) 492
BcnI CCSGG 2 cut(s) 177, 468
BcoDI GTCTC 1 cut(s) 570
BfaI CTAG 1 cut(s) 555
BfmI CTRYAG 1 cut(s) 192
BisI GCNGC 1 cut(s) 559
BlsI GCNGC 1 cut(s) 560
Bme1390I CCNGG 3 cut(s) 177, 468, 492
Bme18I GGWCC 1 cut(s) 213
BmgT120I GGNCC 1 cut(s) 213
BmrFI CCNGG 3 cut(s) 177, 468, 492
BmsI GCATC 1 cut(s) 235
Bpu10I CCTNAGC 1 cut(s) 381
Bpu14I TTCGAA 1 cut(s) 322
BpuEI CTTGAG 2 cut(s) 601, 689
BpuMI CCSGG 2 cut(s) 177, 468
BsaJI CCNNGG 4 cut(s) 176, 216, 466, 676
BsaXI ACNNNNNCTCC 2 cut(s) 508, 538
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 217, 621, 726
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 3 cut(s) 386, 424, 491
BseBI CCWGG 1 cut(s) 492
BseDI CCNNGG 4 cut(s) 176, 216, 466, 676
BseGI GGATG 4 cut(s) 10, 20, 399, 699
BseLI CCNNNNNNNGG 4 cut(s) 90, 217, 621, 726
BseMII CTCAG 2 cut(s) 146, 395
BseNI ACTGG 3 cut(s) 386, 424, 491
BsgI GTGCAG 1 cut(s) 360
Bsh1236I CGCG 1 cut(s) 514
BshFI GGCC 6 cut(s) 175, 210, 312, 353, 373, 490
BsiSI CCGG 4 cut(s) 84, 176, 211, 468
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 4 cut(s) 90, 217, 621, 726
BsmAI GTCTC 1 cut(s) 570
BsmFI GGGAC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 6 cut(s) 175, 210, 312, 353, 373, 490
Bsp119I TTCGAA 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 676
Bsp143I GATC 3 cut(s) 222, 363, 504
BspACI CCGC 3 cut(s) 514, 528, 559
BspANI GGCC 6 cut(s) 175, 210, 312, 353, 373, 490
BspCNI CTCAG 2 cut(s) 145, 394
BspFNI CGCG 1 cut(s) 514
BspPI GGATC 1 cut(s) 230
BspQI GCTCTTC 2 cut(s) 430, 502
BspT104I TTCGAA 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 210
BsrI ACTGG 3 cut(s) 386, 424, 491
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 4 cut(s) 176, 216, 466, 676
BssMI GATC 3 cut(s) 222, 363, 504
BssT1I CCWWGG 1 cut(s) 676
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 2 cut(s) 238, 729
Bst6I CTCTTC 2 cut(s) 430, 502
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 4 cut(s) 164, 310, 375, 460
BstDEI CTNAG 2 cut(s) 132, 381
BstF5I GGATG 4 cut(s) 10, 20, 399, 699
BstFNI CGCG 1 cut(s) 514
BstKTI GATC 3 cut(s) 225, 366, 507
BstMAI GTCTC 1 cut(s) 570
BstMBI GATC 3 cut(s) 222, 363, 504
BstMWI GCNNNNNNNGC 2 cut(s) 145, 172
BstNI CCWGG 1 cut(s) 492
BstSCI CCNGG 3 cut(s) 175, 466, 490
BstSFI CTRYAG 1 cut(s) 192
BstUI CGCG 1 cut(s) 514
BsuRI GGCC 6 cut(s) 175, 210, 312, 353, 373, 490
BtsCI GGATG 4 cut(s) 10, 20, 399, 699
Cac8I GCNNGC 4 cut(s) 164, 310, 375, 460
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 213
CpoI CGGWCCG 1 cut(s) 213
Csp6I GTAC 1 cut(s) 71
CspI CGGWCCG 1 cut(s) 213
CviAII CATG 1 cut(s) 540
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 2 cut(s) 132, 381
DpnI GATC 3 cut(s) 224, 365, 506
DpnII GATC 3 cut(s) 222, 363, 504
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 2 cut(s) 430, 502
EarI CTCTTC 2 cut(s) 430, 502
Eco130I CCWWGG 1 cut(s) 676
Eco147I AGGCCT 2 cut(s) 353, 373
Eco24I GRGCYC 1 cut(s) 676
Eco47I GGWCC 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 676
ErhI CCWWGG 1 cut(s) 676
FaeI CATG 1 cut(s) 543
FaiI YATR 7 cut(s) 59, 159, 449, 541, 597, 610, 612
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 1 cut(s) 194
FatI CATG 1 cut(s) 539
FauI CCCGC 1 cut(s) 521
FauNDI CATATG 1 cut(s) 610
Fnu4HI GCNGC 1 cut(s) 559
FokI GGATG 4 cut(s) 7, 17, 406, 686
FriOI GRGCYC 1 cut(s) 676
Fsp4HI GCNGC 1 cut(s) 559
FspBI CTAG 1 cut(s) 555
GluI GCNGC 1 cut(s) 559
HaeIII GGCC 6 cut(s) 175, 210, 312, 353, 373, 490
HapII CCGG 4 cut(s) 84, 176, 211, 468
Hin1II CATG 1 cut(s) 543
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 3 cut(s) 327, 401, 658
HpaI GTTAAC 1 cut(s) 417
HpaII CCGG 4 cut(s) 84, 176, 211, 468
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 2 cut(s) 135, 217
Hpy8I GTNNAC 1 cut(s) 417
HpyAV CCTTC 1 cut(s) 445
HpyCH4III ACNGT 2 cut(s) 238, 729
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 5 cut(s) 116, 248, 359, 377, 543
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 172
HpyF3I CTNAG 2 cut(s) 132, 381
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 1 cut(s) 543
KspAI GTTAAC 1 cut(s) 417
Kzo9I GATC 3 cut(s) 222, 363, 504
LguI GCTCTTC 2 cut(s) 430, 502
LmnI GCTCC 1 cut(s) 624
LweI GCATC 1 cut(s) 235
MaeI CTAG 1 cut(s) 555
MaeII ACGT 1 cut(s) 14
MalI GATC 3 cut(s) 224, 365, 506
MboI GATC 3 cut(s) 222, 363, 504
MboII GAAGA 5 cut(s) 336, 444, 447, 489, 560
MfeI CAATTG 1 cut(s) 117
MhlI GDGCHC 1 cut(s) 676
MluCI AATT 2 cut(s) 117, 533
MmeI TCCRAC 1 cut(s) 593
MseI TTAA 4 cut(s) 345, 416, 476, 693
MspI CCGG 4 cut(s) 84, 176, 211, 468
MspR9I CCNGG 3 cut(s) 177, 468, 492
MunI CAATTG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 1 cut(s) 492
MvnI CGCG 1 cut(s) 514
MwoI GCNNNNNNNGC 2 cut(s) 145, 172
NciI CCSGG 2 cut(s) 177, 468
NdeI CATATG 1 cut(s) 610
NdeII GATC 3 cut(s) 222, 363, 504
NlaIII CATG 1 cut(s) 543
NspV TTCGAA 1 cut(s) 322
PceI AGGCCT 2 cut(s) 353, 373
PciSI GCTCTTC 2 cut(s) 430, 502
PctI GAATGC 1 cut(s) 709
PfeI GAWTC 3 cut(s) 327, 401, 658
PkrI GCNGC 1 cut(s) 560
Psp6I CCWGG 1 cut(s) 490
PspGI CCWGG 1 cut(s) 490
PspPI GGNCC 1 cut(s) 213
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
Rsr2I CGGWCCG 1 cut(s) 213
RsrII CGGWCCG 1 cut(s) 213
SapI GCTCTTC 2 cut(s) 430, 502
SaqAI TTAA 4 cut(s) 345, 416, 476, 693
SatI GCNGC 1 cut(s) 559
Sau3AI GATC 3 cut(s) 222, 363, 504
Sau96I GGNCC 1 cut(s) 213
ScrFI CCNGG 3 cut(s) 177, 468, 492
SduI GDGCHC 1 cut(s) 676
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 1 cut(s) 192
SfuI TTCGAA 1 cut(s) 322
SinI GGWCC 1 cut(s) 213
SmlI CTYRAG 2 cut(s) 616, 704
SmoI CTYRAG 2 cut(s) 616, 704
Sse9I AATT 2 cut(s) 117, 533
SseBI AGGCCT 2 cut(s) 353, 373
SsiI CCGC 3 cut(s) 514, 528, 559
SspMI CTAG 1 cut(s) 555
StuI AGGCCT 2 cut(s) 353, 373
StyD4I CCNGG 3 cut(s) 175, 466, 490
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 2 cut(s) 238, 729
TaiI ACGT 1 cut(s) 17
TaqI TCGA 2 cut(s) 322, 330
TasI AATT 2 cut(s) 117, 533
TauI GCSGC 1 cut(s) 561
TfiI GAWTC 3 cut(s) 327, 401, 658
Tru1I TTAA 4 cut(s) 345, 416, 476, 693
Tru9I TTAA 4 cut(s) 345, 416, 476, 693
TspDTI ATGAA 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 213
XspI CTAG 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.