AT5G01650

macrophage migration inhibitory factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007982
Physical Location & Seq
Reverse (-)
242450 .. 244111
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01650.2

Sequence Viewer

Length: 348 bp
ATGCCGTGCCTCAACCTCTCCACCAACGTTAACCTTGACGGCGTCGATACATCTTCCATTCTCTCGGAAGCTTCCTCCACCGTCGCGAAAATCATCGGCAAGCCTGAGAACTATGTGATGATTGTCTTGAAAGGCTCAGTGCCTATGTCATTTGGCGGGACCGAGGATCCTGCAGCTTATGGTGAATTAGTTTCTATCGGTGGCCTTAATGCGGATGTGAACAAGAAGCTAAGCGCTGCTGTTTCCGCCATTCTTGAGACTAAGCTATCGGTGCCCAAGTCTCGATTCTTCCTCAAGTTTTATGACACCAAGGGATCCTTCTTTGGTTGGAACGGGGCGACTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

115

Amino Acids

12.11

Weight (kDa)

6.17

Isoelectric Point (pI)

31.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 104 1.5e-19 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AccII CGCG 1 cut(s) 86
AciI CCGC 3 cut(s) 156, 212, 246
AclI AACGTT 1 cut(s) 27
AclWI GGATC 4 cut(s) 161, 174, 309, 322
AcyI GRCGYC 1 cut(s) 42
AfeI AGCGCT 1 cut(s) 235
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 1 cut(s) 130
AjuI GAANNNNNNNTTGG 4 cut(s) 269, 301, 302, 334
AluBI AGCT 4 cut(s) 71, 176, 229, 265
AluI AGCT 4 cut(s) 71, 176, 229, 265
Alw26I GTCTC 2 cut(s) 251, 285
AlwI GGATC 4 cut(s) 161, 174, 309, 322
Aor51HI AGCGCT 1 cut(s) 235
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 2 cut(s) 173, 236
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 159
AsuHPI GGTGA 1 cut(s) 194
AvaII GGWCC 1 cut(s) 159
BaeGI GKGCMC 1 cut(s) 276
BamHI GGATCC 2 cut(s) 166, 314
BanI GGYRCC 1 cut(s) 271
BbvI GCAGC 2 cut(s) 185, 223
BceAI ACGGC 1 cut(s) 55
BcgI CGANNNNNNTGC 2 cut(s) 152, 186
BcoDI GTCTC 2 cut(s) 251, 285
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 237
BisI GCNGC 2 cut(s) 174, 237
BlpI GCTNAGC 1 cut(s) 230
BlsI GCNGC 2 cut(s) 175, 238
Bme18I GGWCC 1 cut(s) 159
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 4 cut(s) 160, 168, 273, 316
Bpu1102I GCTNAGC 1 cut(s) 230
BpuEI CTTGAG 2 cut(s) 275, 278
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 2 cut(s) 162, 309
Bsc4I CCNNNNNNNGG 1 cut(s) 211
BseDI CCNNGG 2 cut(s) 162, 309
BseGI GGATG 1 cut(s) 220
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 96, 150
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 2 cut(s) 185, 223
Bsh1236I CGCG 1 cut(s) 86
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 271
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 2 cut(s) 251, 285
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 2 cut(s) 166, 314
Bsp1720I GCTNAGC 1 cut(s) 230
Bsp68I TCGCGA 1 cut(s) 86
BspACI CCGC 3 cut(s) 156, 212, 246
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 2 cut(s) 97, 149
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 4 cut(s) 160, 168, 273, 316
BspMAI CTGCAG 1 cut(s) 175
BspPI GGATC 4 cut(s) 161, 174, 309, 322
BspT107I GGYRCC 1 cut(s) 271
BssECI CCNNGG 2 cut(s) 162, 309
BssMI GATC 2 cut(s) 166, 314
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 4 cut(s) 105, 136, 230, 261
BstF5I GGATG 1 cut(s) 220
BstFNI CGCG 1 cut(s) 86
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 2 cut(s) 169, 317
BstMAI GTCTC 2 cut(s) 251, 285
BstMBI GATC 2 cut(s) 166, 314
BstMWI GCNNNNNNNGC 2 cut(s) 245, 271
BstSFI CTRYAG 1 cut(s) 171
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 2 cut(s) 185, 223
BstX2I RGATCY 2 cut(s) 166, 314
BstYI RGATCY 2 cut(s) 166, 314
BsuRI GGCC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 220
BtsIMutI CAGTG 1 cut(s) 144
BtuMI TCGCGA 1 cut(s) 86
Cac8I GCNNGC 1 cut(s) 101
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 159
CseI GACGC 1 cut(s) 31
CviJI RGCY 7 cut(s) 71, 103, 135, 176, 204, 229, 265
CviKI_1 RGCY 7 cut(s) 71, 103, 135, 176, 204, 229, 265
DdeI CTNAG 4 cut(s) 105, 136, 230, 261
DpnI GATC 2 cut(s) 168, 316
DpnII GATC 2 cut(s) 166, 314
EciI GGCGGA 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 159
Eco47III AGCGCT 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
FaiI YATR 4 cut(s) 114, 146, 180, 303
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FaqI GGGAC 1 cut(s) 172
FauI CCCGC 1 cut(s) 149
Fnu4HI GCNGC 2 cut(s) 174, 237
FokI GGATG 1 cut(s) 227
Fsp4HI GCNGC 2 cut(s) 174, 237
GlaI GCGC 1 cut(s) 235
GluI GCNGC 2 cut(s) 174, 237
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 1 cut(s) 204
HgaI GACGC 1 cut(s) 31
HhaI GCGC 1 cut(s) 236
Hin1I GRCGYC 1 cut(s) 42
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 69
HinfI GANTC 2 cut(s) 285, 340
HpaI GTTAAC 1 cut(s) 31
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 2 cut(s) 31, 220
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 4 cut(s) 85, 127, 254, 282
Hpy8I GTNNAC 2 cut(s) 31, 220
Hpy99I CGWCG 2 cut(s) 47, 86
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 173
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 271
HpyF3I CTNAG 4 cut(s) 105, 136, 230, 261
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 42
HspAI GCGC 1 cut(s) 234
KspAI GTTAAC 1 cut(s) 31
Kzo9I GATC 2 cut(s) 166, 314
LpnPI CCDG 2 cut(s) 117, 183
Lsp1109I GCAGC 2 cut(s) 185, 223
MaeII ACGT 1 cut(s) 27
MalI GATC 2 cut(s) 168, 316
MboI GATC 2 cut(s) 166, 314
MboII GAAGA 2 cut(s) 45, 280
MflI RGATCY 2 cut(s) 166, 314
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 1 cut(s) 185
MlyI GAGTC 1 cut(s) 334
MmeI TCCRAC 1 cut(s) 308
MnlI CCTC 5 cut(s) 20, 26, 85, 157, 302
MseI TTAA 3 cut(s) 30, 207, 346
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 2 cut(s) 245, 271
NdeII GATC 2 cut(s) 166, 314
NlaIV GGNNCC 4 cut(s) 160, 168, 273, 316
NruI TCGCGA 1 cut(s) 86
PfeI GAWTC 1 cut(s) 285
PflFI GACNNNGTC 1 cut(s) 41
PkrI GCNGC 2 cut(s) 175, 238
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
Psp1406I AACGTT 1 cut(s) 27
PspN4I GGNNCC 4 cut(s) 160, 168, 273, 316
PspPI GGNCC 1 cut(s) 159
PstI CTGCAG 1 cut(s) 175
PsuI RGATCY 2 cut(s) 166, 314
PsyI GACNNNGTC 1 cut(s) 41
RruI TCGCGA 1 cut(s) 86
SaqAI TTAA 3 cut(s) 30, 207, 346
SatI GCNGC 2 cut(s) 174, 237
Sau3AI GATC 2 cut(s) 166, 314
Sau96I GGNCC 1 cut(s) 159
SchI GAGTC 1 cut(s) 334
SduI GDGCHC 1 cut(s) 276
SetI ASST 7 cut(s) 18, 30, 36, 73, 178, 231, 267
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 1 cut(s) 159
SmlI CTYRAG 2 cut(s) 254, 293
SmoI CTYRAG 2 cut(s) 254, 293
Sse9I AATT 1 cut(s) 185
SsiI CCGC 3 cut(s) 156, 212, 246
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 45, 283
TaqII GACCGA 1 cut(s) 176
TasI AATT 1 cut(s) 185
TfiI GAWTC 1 cut(s) 285
Tru1I TTAA 3 cut(s) 30, 207, 346
Tru9I TTAA 3 cut(s) 30, 207, 346
TscAI CASTG 1 cut(s) 144
TseI GCWGC 2 cut(s) 173, 236
TspRI CASTG 1 cut(s) 144
Tth111I GACNNNGTC 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.