Rh3AG110600

Macrophage migration inhibitory factor homolog isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0007982
Physical Location & Seq
Forward (+)
9334181 .. 9336589
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG110600.1

Sequence Viewer

Length: 357 bp
ATGCCGTGTTTGAACATTTCAGCGAACGTCAGCCTGGAAGGAGTGGACACCTCCTCCATCCTCTCTGAAGCCACCTCCACCGTCGCCAAGATCATCGGCAAACCCGAGGCTTATGTGATGATTGTGCTGAAGGGATCAGTTCCCATATCTTTTGGAGGGACTGAGCAGCCAGCTGCCTATGGTGAGTTGGTCTCCATTGGTGGCCTGAGCCCTGATGTGAACAAGAAGTTGAGTGCTGCGATTTCCTCAATCCTTGAGACCAAGTTGTCTGTGCCCAAGTCGCGATTTTTTCTTAAGTTCTATGATACCAAGGCAAGTCATCTCCCGTTTCTGAGTTTCCAGAGAAAGGGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

118

Amino Acids

12.53

Weight (kDa)

9.1

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 109 4.6e-19 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 265
AccII CGCG 1 cut(s) 283
AclWI GGATC 1 cut(s) 142
AcuI CTGAAG 2 cut(s) 87, 149
AfiI CCNNNNNNNGG 1 cut(s) 346
AflII CTTAAG 1 cut(s) 293
AgsI TTSAA 1 cut(s) 13
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 1 cut(s) 173
AluI AGCT 1 cut(s) 173
Alw26I GTCTC 2 cut(s) 196, 251
AlwI GGATC 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 3 cut(s) 166, 173, 236
AsuHPI GGTGA 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 104
BaeGI GKGCMC 1 cut(s) 276
BanII GRGCYC 1 cut(s) 212
BbvI GCAGC 3 cut(s) 160, 178, 223
BccI CCATC 1 cut(s) 65
BciT130I CCWGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 196, 251
BfrI CTTAAG 1 cut(s) 293
BisI GCNGC 3 cut(s) 167, 174, 237
BlsI GCNGC 3 cut(s) 168, 175, 238
Bme1390I CCNGG 1 cut(s) 35
BmeT110I CYCGRG 1 cut(s) 104
BmrFI CCNGG 1 cut(s) 35
BplI GAGNNNNNCTC 2 cut(s) 176, 208
Bpu10I CCTNAGC 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 275
BsaI GGTCTC 2 cut(s) 196, 251
BsaJI CCNNGG 2 cut(s) 105, 309
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 105, 309
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMII CTCAG 3 cut(s) 153, 197, 323
BseRI GAGGAG 1 cut(s) 43
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 3 cut(s) 160, 178, 223
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 2 cut(s) 196, 251
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 2 cut(s) 196, 251
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 212, 276
Bsp143I GATC 2 cut(s) 90, 134
Bsp68I TCGCGA 1 cut(s) 283
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 3 cut(s) 154, 198, 324
BspFNI CGCG 1 cut(s) 283
BspPI GGATC 1 cut(s) 142
BspTI CTTAAG 1 cut(s) 293
BspTNI GGTCTC 2 cut(s) 196, 251
BssECI CCNNGG 2 cut(s) 105, 309
BssMI GATC 2 cut(s) 90, 134
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 82
BstAFI CTTAAG 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 3 cut(s) 162, 206, 332
BstF5I GGATG 1 cut(s) 57
BstFNI CGCG 1 cut(s) 283
BstKTI GATC 2 cut(s) 93, 137
BstMAI GTCTC 2 cut(s) 196, 251
BstMBI GATC 2 cut(s) 90, 134
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstNI CCWGG 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 3 cut(s) 160, 178, 223
BsuRI GGCC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 57
BtuMI TCGCGA 1 cut(s) 283
Cac8I GCNNGC 1 cut(s) 171
CviJI RGCY 7 cut(s) 33, 71, 110, 169, 173, 204, 210
CviKI_1 RGCY 7 cut(s) 33, 71, 110, 169, 173, 204, 210
DdeI CTNAG 3 cut(s) 162, 206, 332
DpnI GATC 2 cut(s) 92, 136
DpnII GATC 2 cut(s) 90, 134
DrdI GACNNNNNNGTC 1 cut(s) 265
DseDI GACNNNNNNGTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 212
Eco31I GGTCTC 2 cut(s) 196, 251
Eco57I CTGAAG 2 cut(s) 87, 149
Eco88I CYCGRG 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 212
ErhI CCWWGG 1 cut(s) 309
FaiI YATR 4 cut(s) 114, 146, 180, 303
FaqI GGGAC 1 cut(s) 172
Fnu4HI GCNGC 3 cut(s) 167, 174, 237
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 3 cut(s) 167, 174, 237
GluI GCNGC 3 cut(s) 167, 174, 237
HaeIII GGCC 1 cut(s) 204
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 2 cut(s) 46, 220
Hpy188I TCNGA 2 cut(s) 67, 333
Hpy188III TCNNGA 2 cut(s) 282, 340
Hpy8I GTNNAC 2 cut(s) 46, 220
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 32, 124
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpyF3I CTNAG 3 cut(s) 162, 206, 332
HpySE526I ACGT 1 cut(s) 27
Kzo9I GATC 2 cut(s) 90, 134
LpnPI CCDG 6 cut(s) 20, 47, 183, 218, 225, 353
Lsp1109I GCAGC 3 cut(s) 160, 178, 223
MaeII ACGT 1 cut(s) 27
MalI GATC 2 cut(s) 92, 136
MboI GATC 2 cut(s) 90, 134
MhlI GDGCHC 2 cut(s) 212, 276
MnlI CCTC 7 cut(s) 61, 64, 71, 85, 100, 149, 256
MseI TTAA 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 173
MspCI CTTAAG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 35
MvaI CCWGG 1 cut(s) 35
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 1 cut(s) 280
NdeII GATC 2 cut(s) 90, 134
NruI TCGCGA 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PkrI GCNGC 3 cut(s) 168, 175, 238
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 173
RruI TCGCGA 1 cut(s) 283
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 3 cut(s) 167, 174, 237
Sau3AI GATC 2 cut(s) 90, 134
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 2 cut(s) 212, 276
SetI ASST 4 cut(s) 30, 53, 77, 175
SmlI CTYRAG 2 cut(s) 254, 293
SmoI CTYRAG 2 cut(s) 254, 293
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 30
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseI GCWGC 3 cut(s) 166, 173, 236
Vha464I CTTAAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.