MD04G1166600.v1.1

Macrophage migration inhibitory factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007982
Physical Location & Seq
Reverse (-)
25685273 .. 25687342
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1166600.v1.1.491

Sequence Viewer

Length: 348 bp
ATGCCGTGCCTGAACATTTCAGCTAACGTGAACCTGGACGGCGTGGACACCTCCGCCATCCTCTCCGAAGCCACCTCCACCGTTGCCAAGATCATCGGCAAGCCCGAGGCTTATGTGATGATTGTGCTGAAGGGATCTGTACCCATATCTTTTGGTGGGACTGAGCAGCCAGCTGCATACGCTGAGTTGGTCTCCATTGGTGGCCTTAATCCTGATGTGAACAAGAAGCTAAGTGCTGCAATTGCCGCAATTCTTGAGACAAAGTTGTCTGTGCCCAAGTCACGATTTTTTCTCAAGTTCTATGACACCAAGGGTTCCAACTTTGGATGGAATGGGTCTACCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

116

Amino Acids

12.09

Weight (kDa)

7.81

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 115 1.1e-22 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 265
AccI GTMKAC 1 cut(s) 338
AciI CCGC 2 cut(s) 54, 246
AclWI GGATC 1 cut(s) 142
AcuI CTGAAG 1 cut(s) 149
AfaI GTAC 1 cut(s) 141
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 3 cut(s) 23, 173, 229
AluI AGCT 3 cut(s) 23, 173, 229
Alw26I GTCTC 2 cut(s) 196, 251
AlwI GGATC 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 3 cut(s) 166, 173, 236
AvaI CYCGRG 1 cut(s) 104
BaeGI GKGCMC 1 cut(s) 276
BbvI GCAGC 3 cut(s) 160, 178, 223
BccI CCATC 2 cut(s) 65, 321
BceAI ACGGC 1 cut(s) 55
BciT130I CCWGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 196, 251
BisI GCNGC 4 cut(s) 167, 174, 237, 246
BlsI GCNGC 4 cut(s) 168, 175, 238, 247
Bme1390I CCNGG 1 cut(s) 35
BmeT110I CYCGRG 1 cut(s) 104
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 1 cut(s) 35
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpuEI CTTGAG 2 cut(s) 275, 278
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 105, 309
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 105, 309
BseGI GGATG 2 cut(s) 57, 332
BseMII CTCAG 2 cut(s) 153, 174
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 3 cut(s) 160, 178, 223
BshFI GGCC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 172
BsmAI GTCTC 2 cut(s) 196, 251
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 196
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 2 cut(s) 90, 134
BspACI CCGC 2 cut(s) 54, 246
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 2 cut(s) 154, 175
BspLI GGNNCC 1 cut(s) 316
BspPI GGATC 1 cut(s) 142
BspTNI GGTCTC 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 105, 309
BssMI GATC 2 cut(s) 90, 134
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 82
BstC8I GCNNGC 2 cut(s) 101, 171
BstDEI CTNAG 3 cut(s) 162, 183, 230
BstF5I GGATG 2 cut(s) 57, 332
BstKTI GATC 2 cut(s) 93, 137
BstMAI GTCTC 2 cut(s) 196, 251
BstMBI GATC 2 cut(s) 90, 134
BstMWI GCNNNNNNNGC 3 cut(s) 179, 242, 245
BstNI CCWGG 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 3 cut(s) 160, 178, 223
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
BsuRI GGCC 1 cut(s) 204
BtsCI GGATG 2 cut(s) 57, 332
Cac8I GCNNGC 2 cut(s) 101, 171
Csp6I GTAC 1 cut(s) 140
CviJI RGCY 8 cut(s) 23, 71, 103, 110, 169, 173, 204, 229
CviKI_1 RGCY 8 cut(s) 23, 71, 103, 110, 169, 173, 204, 229
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 3 cut(s) 162, 183, 230
DpnI GATC 2 cut(s) 92, 136
DpnII GATC 2 cut(s) 90, 134
DrdI GACNNNNNNGTC 1 cut(s) 265
DseDI GACNNNNNNGTC 1 cut(s) 265
EciI GGCGGA 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 149
Eco88I CYCGRG 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
FaiI YATR 4 cut(s) 114, 146, 178, 303
FaqI GGGAC 1 cut(s) 172
FblI GTMKAC 1 cut(s) 338
Fnu4HI GCNGC 4 cut(s) 167, 174, 237, 246
FokI GGATG 2 cut(s) 44, 339
Fsp4HI GCNGC 4 cut(s) 167, 174, 237, 246
GluI GCNGC 4 cut(s) 167, 174, 237, 246
HaeIII GGCC 1 cut(s) 204
Hpy166II GTNNAC 4 cut(s) 31, 46, 220, 339
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 3 cut(s) 212, 254, 282
Hpy8I GTNNAC 4 cut(s) 31, 46, 220, 339
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 2 cut(s) 176, 239
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 242, 245
HpyF3I CTNAG 3 cut(s) 162, 183, 230
HpySE526I ACGT 1 cut(s) 27
Kzo9I GATC 2 cut(s) 90, 134
LpnPI CCDG 5 cut(s) 20, 23, 47, 183, 225
Lsp1109I GCAGC 3 cut(s) 160, 178, 223
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 279
MalI GATC 2 cut(s) 92, 136
MboI GATC 2 cut(s) 90, 134
MfeI CAATTG 1 cut(s) 240
MflI RGATCY 1 cut(s) 134
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 2 cut(s) 240, 249
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 4 cut(s) 61, 71, 85, 100
MseI TTAA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 173
MspR9I CCNGG 1 cut(s) 35
MunI CAATTG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 35
MwoI GCNNNNNNNGC 3 cut(s) 179, 242, 245
NdeII GATC 2 cut(s) 90, 134
NlaIV GGNNCC 1 cut(s) 316
NmuCI GTSAC 1 cut(s) 279
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PkrI GCNGC 4 cut(s) 168, 175, 238, 247
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 316
PsuI RGATCY 1 cut(s) 134
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 4 cut(s) 167, 174, 237, 246
Sau3AI GATC 2 cut(s) 90, 134
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 276
SetI ASST 8 cut(s) 25, 30, 36, 53, 77, 175, 231, 344
SmlI CTYRAG 2 cut(s) 254, 293
SmoI CTYRAG 2 cut(s) 254, 293
Sse9I AATT 2 cut(s) 240, 249
SsiI CCGC 2 cut(s) 54, 246
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 30
TasI AATT 2 cut(s) 240, 249
TauI GCSGC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TseFI GTSAC 1 cut(s) 279
TseI GCWGC 3 cut(s) 166, 173, 236
Tsp45I GTSAC 1 cut(s) 279
XmiI GTMKAC 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.