Rroxscaffold_6G00418460

Macrophage migration inhibitory factor homolog isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007982
Physical Location & Seq
Reverse (-)
39996410 .. 39998330
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418460.1

Sequence Viewer

Length: 366 bp
ATGCCGTGTTTGAACATTTCAGCGAACGTCAGCCTGGAAGGAGTGGACACCTCCTCCATCCTCTCTGAAGCCACCTCCACCGTCGCCAAGATCATCGGCAAACCCGAGGCTTATGTGATGATTGTGCTGAAGGGATCGGTTCCCATATCTTTTGGAGGGACTGAGCAGCCAGCTGCCTATGGTGAATTGGTCTCCATTGGTGGCCTGAGCCCTGATGTGAACAAGAAGTTGAGTGCTGCGATTTCCTCAATCCTTGAGACCAAGTTGTCTGTGCCCAAGTCGCGATTTTTTCTGAAGTTCTATGATACCAAGGCGCATCAGAGTCAAGAACATGCTCAGTGTTTGCATGCTTTACACCAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

121

Amino Acids

12.86

Weight (kDa)

6.89

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 107 1e-18 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 265
AccII CGCG 1 cut(s) 283
AclWI GGATC 1 cut(s) 142
AcuI CTGAAG 3 cut(s) 87, 149, 314
AgsI TTSAA 1 cut(s) 13
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 1 cut(s) 173
AluI AGCT 1 cut(s) 173
Alw26I GTCTC 2 cut(s) 196, 251
AlwI GGATC 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 3 cut(s) 166, 173, 236
AspLEI GCGC 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 104
BaeGI GKGCMC 1 cut(s) 276
BanII GRGCYC 1 cut(s) 212
BbvI GCAGC 3 cut(s) 160, 178, 223
BccI CCATC 1 cut(s) 65
BciT130I CCWGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 196, 251
BfaI CTAG 1 cut(s) 364
BisI GCNGC 3 cut(s) 167, 174, 237
BlsI GCNGC 3 cut(s) 168, 175, 238
Bme1390I CCNGG 1 cut(s) 35
BmeT110I CYCGRG 1 cut(s) 104
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 1 cut(s) 35
BmsI GCATC 1 cut(s) 325
Bpu10I CCTNAGC 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 275
BsaI GGTCTC 2 cut(s) 196, 251
BsaJI CCNNGG 2 cut(s) 105, 309
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 105, 309
BseGI GGATG 1 cut(s) 57
BseMII CTCAG 3 cut(s) 153, 197, 350
BseRI GAGGAG 1 cut(s) 43
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 3 cut(s) 160, 178, 223
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 172
BsmAI GTCTC 2 cut(s) 196, 251
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 2 cut(s) 196, 251
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 212, 276
Bsp143I GATC 2 cut(s) 90, 134
Bsp68I TCGCGA 1 cut(s) 283
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 3 cut(s) 154, 198, 349
BspFNI CGCG 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 141
BspPI GGATC 1 cut(s) 142
BspTNI GGTCTC 2 cut(s) 196, 251
BssECI CCNNGG 2 cut(s) 105, 309
BssMI GATC 2 cut(s) 90, 134
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 82
BstC8I GCNNGC 2 cut(s) 171, 348
BstDEI CTNAG 3 cut(s) 162, 206, 336
BstF5I GGATG 1 cut(s) 57
BstFNI CGCG 1 cut(s) 283
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 2 cut(s) 93, 137
BstMAI GTCTC 2 cut(s) 196, 251
BstMBI GATC 2 cut(s) 90, 134
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstNI CCWGG 1 cut(s) 35
BstNSI RCATGY 2 cut(s) 335, 350
BstSCI CCNGG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 3 cut(s) 160, 178, 223
BsuRI GGCC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 344
BtuMI TCGCGA 1 cut(s) 283
Cac8I GCNNGC 2 cut(s) 171, 348
CfoI GCGC 1 cut(s) 316
CviAII CATG 2 cut(s) 332, 347
CviJI RGCY 7 cut(s) 33, 71, 110, 169, 173, 204, 210
CviKI_1 RGCY 7 cut(s) 33, 71, 110, 169, 173, 204, 210
DdeI CTNAG 3 cut(s) 162, 206, 336
DpnI GATC 2 cut(s) 92, 136
DpnII GATC 2 cut(s) 90, 134
DrdI GACNNNNNNGTC 1 cut(s) 265
DseDI GACNNNNNNGTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 212
Eco31I GGTCTC 2 cut(s) 196, 251
Eco57I CTGAAG 3 cut(s) 87, 149, 314
Eco88I CYCGRG 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 212
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 2 cut(s) 335, 350
FaiI YATR 6 cut(s) 114, 146, 180, 303, 333, 348
FaqI GGGAC 1 cut(s) 172
FatI CATG 2 cut(s) 331, 346
Fnu4HI GCNGC 3 cut(s) 167, 174, 237
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 3 cut(s) 167, 174, 237
FspBI CTAG 1 cut(s) 364
GlaI GCGC 1 cut(s) 315
GluI GCNGC 3 cut(s) 167, 174, 237
HaeIII GGCC 1 cut(s) 204
HhaI GCGC 1 cut(s) 316
Hin1II CATG 2 cut(s) 335, 350
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HinfI GANTC 1 cut(s) 322
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 2 cut(s) 46, 220
Hpy188I TCNGA 3 cut(s) 67, 294, 321
Hpy188III TCNNGA 2 cut(s) 282, 326
Hpy8I GTNNAC 2 cut(s) 46, 220
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 32, 124
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpyF3I CTNAG 3 cut(s) 162, 206, 336
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 2 cut(s) 335, 350
HspAI GCGC 1 cut(s) 314
Kzo9I GATC 2 cut(s) 90, 134
LpnPI CCDG 5 cut(s) 20, 47, 183, 218, 225
Lsp1109I GCAGC 3 cut(s) 160, 178, 223
LweI GCATC 1 cut(s) 325
MaeI CTAG 1 cut(s) 364
MaeII ACGT 1 cut(s) 27
MalI GATC 2 cut(s) 92, 136
MboI GATC 2 cut(s) 90, 134
MhlI GDGCHC 2 cut(s) 212, 276
MluCI AATT 1 cut(s) 185
MlyI GAGTC 1 cut(s) 331
MnlI CCTC 7 cut(s) 61, 64, 71, 85, 100, 149, 256
MspA1I CMGCKG 1 cut(s) 173
MspR9I CCNGG 1 cut(s) 35
MvaI CCWGG 1 cut(s) 35
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 1 cut(s) 280
NdeII GATC 2 cut(s) 90, 134
NlaIII CATG 2 cut(s) 335, 350
NlaIV GGNNCC 1 cut(s) 141
NruI TCGCGA 1 cut(s) 283
NspI RCATGY 2 cut(s) 335, 350
PaeI GCATGC 1 cut(s) 350
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PkrI GCNGC 3 cut(s) 168, 175, 238
PleI GAGTC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 173
RruI TCGCGA 1 cut(s) 283
SatI GCNGC 3 cut(s) 167, 174, 237
Sau3AI GATC 2 cut(s) 90, 134
SchI GAGTC 1 cut(s) 331
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 2 cut(s) 212, 276
SetI ASST 4 cut(s) 30, 53, 77, 175
SfaNI GCATC 1 cut(s) 325
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SphI GCATGC 1 cut(s) 350
Sse9I AATT 1 cut(s) 185
SspMI CTAG 1 cut(s) 364
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 30
TasI AATT 1 cut(s) 185
TscAI CASTG 1 cut(s) 344
TseI GCWGC 3 cut(s) 166, 173, 236
TspRI CASTG 1 cut(s) 344
XceI RCATGY 2 cut(s) 335, 350
XspI CTAG 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.