FvH4_6g10620

Macrophage migration inhibitory factor homolog isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007982
Physical Location & Seq
Forward (+)
6375951 .. 6377918
1968 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10620.t1

Sequence Viewer

Length: 357 bp
ATGCCGTGTTTGAACATTTCCGCCAACGTCAGCCTCGAAGGAGTTGACACCTCCTCCATCCTCTCTGAAGCCACCTCCACCGTCGCCAAGATCATCGGCAAACCCGAGGCTTATGTCATGATTGTGCTGAAGGGATCAGTTCCCATATCTTTTGGAGGGACTGAGCAACCAGCTGCTTATGGTGAGTTGGTCTCCATTGGTGGCCTGAGCCCTGATGTGAACAAGAAGTTGAGTGCTGCAATTTCCTCAATCCTTGAGGCCAAGTTGTCTGTGCCTAAGTCAAGATTCTTCCTGAAGTTCTATGATACCAAGGCAAGTCATCTCACCTTTCTGACTTTCCAGAGAAAGGGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

119

Amino Acids

12.52

Weight (kDa)

9.1

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 110 2.9e-19 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 21
AclWI GGATC 1 cut(s) 142
AcuI CTGAAG 3 cut(s) 87, 149, 314
AfiI CCNNNNNNNGG 1 cut(s) 346
AgsI TTSAA 1 cut(s) 13
AluBI AGCT 1 cut(s) 173
AluI AGCT 1 cut(s) 173
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 2 cut(s) 202, 258
ApeKI GCWGC 2 cut(s) 173, 236
AsuHPI GGTGA 2 cut(s) 194, 316
AvaI CYCGRG 1 cut(s) 104
BanII GRGCYC 1 cut(s) 212
BbvI GCAGC 2 cut(s) 160, 223
BccI CCATC 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 196
BisI GCNGC 2 cut(s) 174, 237
BlsI GCNGC 2 cut(s) 175, 238
BmeT110I CYCGRG 1 cut(s) 104
BplI GAGNNNNNCTC 2 cut(s) 176, 208
Bpu10I CCTNAGC 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 275
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 105, 309
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 1 cut(s) 346
BseDI CCNNGG 2 cut(s) 105, 309
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMII CTCAG 2 cut(s) 153, 197
BseRI GAGGAG 1 cut(s) 43
BseXI GCAGC 2 cut(s) 160, 223
BshFI GGCC 2 cut(s) 204, 260
BsiHKCI CYCGRG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 2 cut(s) 204, 260
Bso31I GGTCTC 1 cut(s) 196
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 212
Bsp143I GATC 2 cut(s) 90, 134
BspACI CCGC 1 cut(s) 21
BspANI GGCC 2 cut(s) 204, 260
BspCNI CTCAG 2 cut(s) 154, 198
BspHI TCATGA 1 cut(s) 117
BspPI GGATC 1 cut(s) 142
BspTNI GGTCTC 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 105, 309
BssMI GATC 2 cut(s) 90, 134
BssT1I CCWWGG 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 82
BstDEI CTNAG 3 cut(s) 162, 206, 276
BstF5I GGATG 1 cut(s) 57
BstKTI GATC 2 cut(s) 93, 137
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 2 cut(s) 90, 134
BstV1I GCAGC 2 cut(s) 160, 223
BsuRI GGCC 2 cut(s) 204, 260
BtsCI GGATG 1 cut(s) 57
CciI TCATGA 1 cut(s) 117
CviAII CATG 1 cut(s) 118
CviJI RGCY 7 cut(s) 33, 71, 110, 173, 204, 210, 260
CviKI_1 RGCY 7 cut(s) 33, 71, 110, 173, 204, 210, 260
DdeI CTNAG 3 cut(s) 162, 206, 276
DpnI GATC 2 cut(s) 92, 136
DpnII GATC 2 cut(s) 90, 134
EciI GGCGGA 1 cut(s) 10
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 212
Eco31I GGTCTC 1 cut(s) 196
Eco57I CTGAAG 3 cut(s) 87, 149, 314
Eco88I CYCGRG 1 cut(s) 104
EcoT14I CCWWGG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 212
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 1 cut(s) 121
FaiI YATR 5 cut(s) 114, 119, 146, 180, 303
FaqI GGGAC 1 cut(s) 172
FatI CATG 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 174, 237
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 2 cut(s) 174, 237
GluI GCNGC 2 cut(s) 174, 237
HaeIII GGCC 2 cut(s) 204, 260
Hin1II CATG 1 cut(s) 121
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 1 cut(s) 285
HphI GGTGA 2 cut(s) 194, 316
Hpy166II GTNNAC 2 cut(s) 46, 220
Hpy188I TCNGA 2 cut(s) 67, 333
Hpy188III TCNNGA 4 cut(s) 118, 282, 292, 340
Hpy8I GTNNAC 2 cut(s) 46, 220
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 32, 124
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 239
HpyF3I CTNAG 3 cut(s) 162, 206, 276
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 1 cut(s) 121
Kzo9I GATC 2 cut(s) 90, 134
LpnPI CCDG 5 cut(s) 183, 218, 225, 305, 353
Lsp1109I GCAGC 2 cut(s) 160, 223
MaeII ACGT 1 cut(s) 27
MalI GATC 2 cut(s) 92, 136
MboI GATC 2 cut(s) 90, 134
MboII GAAGA 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 1 cut(s) 240
MnlI CCTC 9 cut(s) 44, 61, 64, 71, 85, 100, 149, 250, 256
MslI CAYNNNNRTG 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 173
NdeII GATC 2 cut(s) 90, 134
NlaIII CATG 1 cut(s) 121
PagI TCATGA 1 cut(s) 117
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 102
PfeI GAWTC 1 cut(s) 285
PkrI GCNGC 2 cut(s) 175, 238
PvuII CAGCTG 1 cut(s) 173
RseI CAYNNNNRTG 1 cut(s) 122
SatI GCNGC 2 cut(s) 174, 237
Sau3AI GATC 2 cut(s) 90, 134
SduI GDGCHC 1 cut(s) 212
SetI ASST 5 cut(s) 30, 53, 77, 175, 329
SmiMI CAYNNNNRTG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 1 cut(s) 240
SsiI CCGC 1 cut(s) 21
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 36
TasI AATT 1 cut(s) 240
TfiI GAWTC 1 cut(s) 285
TseI GCWGC 2 cut(s) 173, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.