AT5G03290

isocitrate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009443
Physical Location & Seq
Forward (+)
793974 .. 796218
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03290.1

Sequence Viewer

Length: 1125 bp
ATGACCATGGCAGCAAATCTAGCGCGACGGCTGATCGGAAACCGATCGACTCAGATCCTTGGAGCCGTGAACTCTAGCTCAGGTGCGGCTAGTTCTGTCGCTAGGGCTTTCTGCTCCTCCACCACTCCGATCACCGCAACTCTCTTCCCTGGCGATGGGATCGGTCCCGAGATCGCCGAATCTGTCAAAAAGGTGTTTACAACAGCTGGTGTACCAATTGAGTGGGAAGAACACTATGTTGGGACTGAGATAGATCCGAGAACACAGAGTTTCTTGACATGGGAAAGTCTCGAATCTGTGCGTAGAAACAAAGTTGGTTTGAAAGGTCCAATGGCTACTCCAATTGGGAAAGGTCACCGTTCTTTGAACCTGACTCTTAGGAAAGAGCTTAATCTATATGCCAATGTTAGACCTTGTTACAGTCTTCCTGGTTACAAAACTCGTTACGATGATGTTGATCTCATTACCATCAGAGAAAACACGGAAGGAGAATACAGTGGCCTTGAACATCAGGTTGTTAGAGGTGTGGTGGAAAGTCTTAAAATCATTACCCGTCAGGCGAGTTTGAGAGTTGCAGAGTATGCTTTTCTCTATGCCAAGACTCATGGAAGAGAGAGAGTTTCTGCTATCCACAAAGCCAACATTATGCAGAAAACTGACGGTCTCTTTCTCAAGTGTTGTCGTGAGGTTGCTGAGAAGTATCCTGAGATCACTTACGAGGAGGTCGTTATTGACAACTGCTGTATGATGCTTGTGAAAAACCCAGCACTTTTTGATGTCTTGGTGATGCCAAACCTGTACGGTGACATTATTAGCGATCTGTGTGCCGGATTGGTTGGAGGACTAGGATTGACTCCAAGTTGTAATATTGGAGAAGATGGTGTAGCCCTTGCTGAAGCAGTTCACGGTTCTGCACCTGATATCGCTGGAAAGAACTTGGCGAACCCAACCGCGTTGCTTCTGAGTGGAGTGATGATGTTGCGTCACCTGAAGTTCAACGAACAAGCAGAACAAATCCACAGTGCAATCATCAACACCATAGCAGAGGGCAAATACAGAACCGCGGATCTTGGAGGTAGTTCGACCACCACGGAATTCACAAAGGCAATCTGTGATCATCTCTGA

Protein Analysis

374

Amino Acids

40.62

Weight (kDa)

6.33

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 47 - 370 1.6e-107 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 25, 953, 1064
AciI CCGC 5 cut(s) 86, 135, 951, 1062, 1064
AclWI GGATC 4 cut(s) 49, 167, 248, 1074
AcsI RAATTY 1 cut(s) 1094
AcuI CTGAAG 2 cut(s) 915, 1010
AfaI GTAC 2 cut(s) 213, 800
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 4 cut(s) 322, 367, 506, 997
AjnI CCWGG 2 cut(s) 148, 427
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AluBI AGCT 3 cut(s) 78, 206, 388
AluI AGCT 3 cut(s) 78, 206, 388
Alw26I GTCTC 2 cut(s) 293, 668
AlwI GGATC 4 cut(s) 49, 167, 248, 1074
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 1 cut(s) 499
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 1 cut(s) 1094
Asp700I GAANNNNTTC 1 cut(s) 900
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 2 cut(s) 164, 326
AsuHPI GGTGA 5 cut(s) 124, 347, 796, 815, 977
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 164, 326
BbsI GAAGAC 1 cut(s) 416
BbvI GCAGC 1 cut(s) 23
BccI CCATC 3 cut(s) 149, 476, 872
BceAI ACGGC 2 cut(s) 44, 50
BcgI CGANNNNNNTGC 2 cut(s) 806, 840
BciT130I CCWGG 2 cut(s) 150, 429
BciVI GTATCC 1 cut(s) 711
BclI TGATCA 1 cut(s) 1114
BcoDI GTCTC 2 cut(s) 293, 668
BfaI CTAG 5 cut(s) 20, 75, 90, 102, 845
BfuI GTATCC 1 cut(s) 711
BisI GCNGC 2 cut(s) 12, 87
BlsI GCNGC 2 cut(s) 13, 88
Bme1390I CCNGG 2 cut(s) 150, 429
Bme18I GGWCC 2 cut(s) 164, 326
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 164, 326
BmiI GGNNCC 2 cut(s) 64, 166
BmrFI CCNGG 2 cut(s) 150, 429
BmsI GCATC 2 cut(s) 738, 777
BpiI GAAGAC 1 cut(s) 416
Bpu10I CCTNAGC 1 cut(s) 79
BpuEI CTTGAG 1 cut(s) 656
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 668
BsaJI CCNNGG 5 cut(s) 6, 58, 148, 1062, 1089
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 2 cut(s) 150, 429
BseDI CCNNGG 5 cut(s) 6, 58, 148, 1062, 1089
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMII CTCAG 6 cut(s) 65, 93, 237, 684, 696, 953
BseRI GAGGAG 2 cut(s) 106, 734
BseXI GCAGC 1 cut(s) 23
BseYI CCCAGC 1 cut(s) 763
BsgI GTGCAG 1 cut(s) 897
Bsh1236I CGCG 3 cut(s) 25, 953, 1064
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 1 cut(s) 501
BsiEI CGRYCG 1 cut(s) 47
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 1 cut(s) 828
BslFI GGGAC 2 cut(s) 150, 256
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 293, 668
BsmFI GGGAC 2 cut(s) 150, 256
BsnI GGCC 1 cut(s) 501
Bso31I GGTCTC 1 cut(s) 668
BsoBI CYCGRG 1 cut(s) 167
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 5 cut(s) 86, 135, 951, 1062, 1064
BspANI GGCC 1 cut(s) 501
BspCNI CTCAG 6 cut(s) 64, 92, 238, 685, 697, 954
BspFNI CGCG 3 cut(s) 25, 953, 1064
BspLI GGNNCC 2 cut(s) 64, 166
BspPI GGATC 4 cut(s) 49, 167, 248, 1074
BspTNI GGTCTC 1 cut(s) 668
BssECI CCNNGG 5 cut(s) 6, 58, 148, 1062, 1089
BssT1I CCWWGG 2 cut(s) 6, 58
Bst2UI CCWGG 2 cut(s) 150, 429
Bst4CI ACNGT 7 cut(s) 359, 422, 497, 662, 803, 908, 1022
Bst6I CTCTTC 2 cut(s) 149, 604
BstAPI GCANNNNNTGC 1 cut(s) 581
BstDEI CTNAG 7 cut(s) 51, 79, 246, 377, 693, 705, 962
BstDSI CCRYGG 3 cut(s) 6, 1062, 1089
BstEII GGTNACC 1 cut(s) 353
BstFNI CGCG 3 cut(s) 25, 953, 1064
BstHHI GCGC 1 cut(s) 25
BstMAI GTCTC 2 cut(s) 293, 668
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 2 cut(s) 20, 581
BstNI CCWGG 2 cut(s) 150, 429
BstPI GGTNACC 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 148, 427
BstUI CGCG 3 cut(s) 25, 953, 1064
BstV1I GCAGC 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 3 cut(s) 54, 253, 1066
BstXI CCANNNNNNTGG 1 cut(s) 222
BstYI RGATCY 3 cut(s) 54, 253, 1066
BsuI GTATCC 1 cut(s) 711
BsuRI GGCC 1 cut(s) 501
BtgI CCRYGG 3 cut(s) 6, 1062, 1089
BtgZI GCGATG 1 cut(s) 168
BtsIMutI CAGTG 2 cut(s) 502, 1027
CfoI GCGC 1 cut(s) 25
Cfr13I GGNCC 2 cut(s) 164, 326
Cfr42I CCGCGG 1 cut(s) 1065
CseI GACGC 1 cut(s) 971
Csp6I GTAC 2 cut(s) 212, 799
CviAII CATG 3 cut(s) 7, 279, 605
CviQI GTAC 2 cut(s) 212, 799
DdeI CTNAG 7 cut(s) 51, 79, 246, 377, 693, 705, 962
Eam1104I CTCTTC 2 cut(s) 149, 604
EarI CTCTTC 2 cut(s) 149, 604
Eco130I CCWWGG 2 cut(s) 6, 58
Eco31I GGTCTC 1 cut(s) 668
Eco32I GATATC 1 cut(s) 922
Eco47I GGWCC 2 cut(s) 164, 326
Eco57I CTGAAG 2 cut(s) 915, 1010
Eco88I CYCGRG 1 cut(s) 167
Eco91I GGTNACC 1 cut(s) 353
EcoO65I GGTNACC 1 cut(s) 353
EcoRI GAATTC 1 cut(s) 1094
EcoRII CCWGG 2 cut(s) 148, 427
EcoRV GATATC 1 cut(s) 922
EcoT14I CCWWGG 2 cut(s) 6, 58
ErhI CCWWGG 2 cut(s) 6, 58
FaeI CATG 3 cut(s) 10, 282, 608
FaqI GGGAC 2 cut(s) 150, 256
FatI CATG 3 cut(s) 6, 278, 604
FbaI TGATCA 1 cut(s) 1114
Fnu4HI GCNGC 2 cut(s) 12, 87
Fsp4HI GCNGC 2 cut(s) 12, 87
FspBI CTAG 5 cut(s) 20, 75, 90, 102, 845
GlaI GCGC 1 cut(s) 24
GluI GCNGC 2 cut(s) 12, 87
GsaI CCCAGC 1 cut(s) 767
HaeIII GGCC 1 cut(s) 501
HapII CCGG 1 cut(s) 828
HgaI GACGC 1 cut(s) 971
HhaI GCGC 1 cut(s) 25
Hin1II CATG 3 cut(s) 10, 282, 608
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HinfI GANTC 6 cut(s) 49, 179, 293, 373, 601, 853
HpaII CCGG 1 cut(s) 828
HphI GGTGA 5 cut(s) 124, 347, 796, 815, 977
Hpy166II GTNNAC 4 cut(s) 70, 198, 212, 904
Hpy188I TCNGA 7 cut(s) 38, 54, 129, 258, 473, 963, 1124
Hpy188III TCNNGA 5 cut(s) 167, 274, 290, 683, 704
Hpy8I GTNNAC 4 cut(s) 70, 198, 212, 904
Hpy99I CGWCG 1 cut(s) 30
HpyAV CCTTC 1 cut(s) 479
HpyCH4III ACNGT 7 cut(s) 359, 422, 497, 662, 803, 908, 1022
HpyCH4V TGCA 4 cut(s) 575, 649, 914, 1025
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 581
HpyF3I CTNAG 7 cut(s) 51, 79, 246, 377, 693, 705, 962
Hsp92II CATG 3 cut(s) 10, 282, 608
HspAI GCGC 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 1114
KspI CCGCGG 1 cut(s) 1065
LmnI GCTCC 2 cut(s) 62, 119
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 2 cut(s) 738, 777
MaeI CTAG 5 cut(s) 20, 75, 90, 102, 845
MaeIII GTNAC 6 cut(s) 353, 416, 431, 443, 803, 983
MboII GAAGA 5 cut(s) 136, 239, 416, 621, 887
MfeI CAATTG 2 cut(s) 216, 342
MflI RGATCY 3 cut(s) 54, 253, 1066
MluCI AATT 3 cut(s) 216, 342, 1094
MlyI GAGTC 4 cut(s) 43, 367, 595, 847
MmeI TCCRAC 1 cut(s) 817
MnlI CCTC 8 cut(s) 127, 515, 679, 712, 715, 833, 1039, 1067
MroXI GAANNNNTTC 1 cut(s) 900
MseI TTAA 2 cut(s) 390, 540
MspA1I CMGCKG 2 cut(s) 206, 1064
MspI CCGG 1 cut(s) 828
MspR9I CCNGG 2 cut(s) 150, 429
MunI CAATTG 2 cut(s) 216, 342
MvaI CCWGG 2 cut(s) 150, 429
MvnI CGCG 3 cut(s) 25, 953, 1064
MwoI GCNNNNNNNGC 2 cut(s) 20, 581
NcoI CCATGG 1 cut(s) 6
NlaIII CATG 3 cut(s) 10, 282, 608
NlaIV GGNNCC 2 cut(s) 64, 166
NmuCI GTSAC 3 cut(s) 353, 803, 983
PcsI WCGNNNNNNNCGW 1 cut(s) 723
PdmI GAANNNNTTC 1 cut(s) 900
PfeI GAWTC 2 cut(s) 179, 293
PkrI GCNGC 2 cut(s) 13, 88
Ple19I CGATCG 1 cut(s) 47
PleI GAGTC 4 cut(s) 43, 367, 595, 847
PpsI GAGTC 4 cut(s) 43, 367, 595, 847
Psp6I CCWGG 2 cut(s) 148, 427
PspEI GGTNACC 1 cut(s) 353
PspFI CCCAGC 1 cut(s) 763
PspGI CCWGG 2 cut(s) 148, 427
PspN4I GGNNCC 2 cut(s) 64, 166
PspPI GGNCC 2 cut(s) 164, 326
PsuI RGATCY 3 cut(s) 54, 253, 1066
PvuI CGATCG 1 cut(s) 47
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 2 cut(s) 213, 800
RsaNI GTAC 2 cut(s) 212, 799
SacII CCGCGG 1 cut(s) 1065
SaqAI TTAA 2 cut(s) 390, 540
SatI GCNGC 2 cut(s) 12, 87
Sau96I GGNCC 2 cut(s) 164, 326
SchI GAGTC 4 cut(s) 43, 367, 595, 847
ScrFI CCNGG 2 cut(s) 150, 429
SfaNI GCATC 2 cut(s) 738, 777
Sfr303I CCGCGG 1 cut(s) 1065
SgrBI CCGCGG 1 cut(s) 1065
SinI GGWCC 2 cut(s) 164, 326
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
Sse9I AATT 3 cut(s) 216, 342, 1094
SsiI CCGC 5 cut(s) 86, 135, 951, 1062, 1064
SspI AATATT 1 cut(s) 868
SspMI CTAG 5 cut(s) 20, 75, 90, 102, 845
StyD4I CCNGG 2 cut(s) 148, 427
StyI CCWWGG 2 cut(s) 6, 58
TaaI ACNGT 7 cut(s) 359, 422, 497, 662, 803, 908, 1022
TaqI TCGA 3 cut(s) 47, 291, 1082
TaqII GACCGA 1 cut(s) 152
TasI AATT 3 cut(s) 216, 342, 1094
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 2 cut(s) 179, 293
Tru1I TTAA 2 cut(s) 390, 540
Tru9I TTAA 2 cut(s) 390, 540
TscAI CASTG 2 cut(s) 502, 1027
TseFI GTSAC 3 cut(s) 353, 803, 983
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 3 cut(s) 353, 803, 983
TspGWI ACGGA 2 cut(s) 497, 1106
TspRI CASTG 2 cut(s) 502, 1027
VpaK11BI GGWCC 2 cut(s) 164, 326
XapI RAATTY 1 cut(s) 1094
XmnI GAANNNNTTC 1 cut(s) 900
XspI CTAG 5 cut(s) 20, 75, 90, 102, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.