Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0009443

Physical Location & Seq

Genomic Coordinates Forward (+)

10112409 .. 10116114

Gene Structure 3706 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g16070.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGGCTTCCCAGCTATTGAGGCGCGTAATCGGAAGCCGCTCCACCACCCACTTTCTCTCCGGCGCCGCGAATCCTTTCCCGTCCTTACTCTCCTCCTCCTCGTCTCCCGCAGCTAGGGTTTTCTCTTCTACCTCCACTCCGATCACCGCCACCCTCTTCCCCGGCGACGGCATCGGCCCCGAGATCGCCGAGTCCGTCAAACAGATATTCAGAGAAGCTGAAGTCCCGATTCAATGGGAAGAACATTATGTTGGGGAGCAAATAGATCCCAGGACGCAGAGTTTTCTGACATGGGAAAGTTTGGAATCAGTACGGAGGAACAAGGTAGGCTTGAAAGGGCCAATGGCCACACCGATTGGGAAAGGTCATCGTTCTTTGAACCTTACACTAAGGAAAGAACTTAACTTGTATGCAAATGTCAGACCTTGTTACAGCCTTCCGGGCTACAAGACTCGTTATGATGATGTTGATCTTGTCACTATCCGTGAGAATACGGAGGGGGAATACAGTGGACTTGAACATCAAGTGGTGAGAGGTGTTGTTGAGAGCCTCAAGATCATTACCCGTCAAGCTAGTTTGAGGGTGGCCGAGTATGCTTTTCATTATGCCCAGGCCCATGGTAGAAAAAGAGTGTCTGCAATTCACAAAGCAAATATAATGCAGAAAACCGATGGCCTTTTTCTCAAGTGTTGCCGTGAGGTTGCAGAGAAGTACCCTGATATAGTATATGAGGAAGTTGTCATTGATAATTGCTGTATGATGCTTGTGAAAAATCCAGCACTTTTCGACGTGTTAGTGATGCCTAACCTATATGGTGATATTATTAGTGACCTTTGCGCTGGGTTGATAGGGGGTCTGGGCTTAACACCAAGCTGCAATATTGGTGAGGGAGGTATTGCCCTGGCTGAAGCTGTACATGGATCAGCACCTGATATCGCTGGAAAGAATTTGGCAAATCCGACTGCTCTGCTTTTGAGTGCTGTCACCATGCTTCGCCATTTGGAGCTCCATGATAAAGCTGATAGGATTCAGGATGCGATCCTGAAGACAATTGCAGAGGGCAAGTACCGTACAGCTGACCTTGGTGGCAAATCTACAACTACTGACTTCACACAAGCAATTATCGGTCATCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004448 GO:0004449 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006102 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009060 GO:0009507 GO:0009536 GO:0009987 GO:0015980 GO:0016491 GO:0016614 GO:0016616 GO:0016999 GO:0017076 GO:0017144 GO:0019752 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0046872 GO:0046914 GO:0055114 GO:0071704 GO:0072350 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00020 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

379

Amino Acids

41.28

Weight (kDa)

6.84

Isoelectric Point (pI)

39.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	51 - 315	5.1e-87	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09810 AT5G03290
fragaria_vesca	FvH4_6g16070 FvH4_6g16070 FvH4_6g16070 FvH4_6g16070
malus_domestica	MD04G1096100.v1.1 MD12G1116100.v1.1
prunus_persica	Prupe.6G235200_v2.0.a1
pyrus_communis	pycom04g09100
rosa_chinensis	RchiOBHm_Chr3g0469901
rosa_laevigata	RLG00000024314
rosa_multiflora	Rmu_sc0009708.1_g000001
rosa_roxburghii	Rroxscaffold_6G00411380
rosa_rugosa	Rorug03G0105200 Rorug03G0105300 Rorug03G0105400
rosa_samantha	Rh3AG154300 Rh3BG178400 Rh3CG169000 Rh3DG196800
rosa_wichuraiana	Rw3G014530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	62
AccBSI	CCGCTC	1 cut(s)	39
AccII	CGCG	2 cut(s)	24, 68
AciI	CCGC	4 cut(s)	37, 66, 108, 147
AclWI	GGATC	3 cut(s)	260, 928, 1033
AcoI	YGGCCR	2 cut(s)	345, 585
AcsI	RAATTY	1 cut(s)	946
AcuI	CTGAAG	3 cut(s)	240, 927, 1064
AcyI	GRCGYC	1 cut(s)	63
AfaI	GTAC	5 cut(s)	312, 713, 915, 1067, 1072
AfiI	CCNNNNNNNGG	2 cut(s)	114, 167
AflIII	ACRYGT	1 cut(s)	789
AgsI	TTSAA	4 cut(s)	233, 334, 379, 518
AjiI	CACGTC	1 cut(s)	790
AjnI	CCWGG	3 cut(s)	269, 609, 900
AjuI	GAANNNNNNNTTGG	2 cut(s)	234, 266
AluBI	AGCT	9 cut(s)	13, 113, 218, 572, 873, 911, 1006, 1019, 1076
AluI	AGCT	9 cut(s)	13, 113, 218, 572, 873, 911, 1006, 1019, 1076
Alw21I	GWGCWC	1 cut(s)	1008
Alw26I	GTCTC	1 cut(s)	108
AlwI	GGATC	3 cut(s)	260, 928, 1033
AlwNI	CAGNNNCTG	1 cut(s)	929
Ama87I	CYCGRG	1 cut(s)	179
AoxI	GGCC	6 cut(s)	175, 338, 345, 585, 612, 673
ApeKI	GCWGC	2 cut(s)	110, 873
ApoI	RAATTY	1 cut(s)	946
Asp700I	GAANNNNTTC	1 cut(s)	74
AspLEI	GCGC	3 cut(s)	24, 65, 839
AspS9I	GGNCC	3 cut(s)	176, 338, 613
AsuC2I	CCSGG	2 cut(s)	162, 441
AsuHPI	GGTGA	5 cut(s)	136, 541, 827, 896, 976
AvaI	CYCGRG	1 cut(s)	179
BalI	TGGCCA	1 cut(s)	347
BanI	GGYRCC	1 cut(s)	62
BanII	GRGCYC	1 cut(s)	1008
BbsI	GAAGAC	1 cut(s)	1052
Bbv12I	GWGCWC	1 cut(s)	1008
BbvI	GCAGC	2 cut(s)	122, 860
BccI	CCATC	1 cut(s)	665
BceAI	ACGGC	2 cut(s)	184, 678
BcgI	CGANNNNNNTGC	2 cut(s)	949, 983
BciT130I	CCWGG	3 cut(s)	271, 611, 902
BcnI	CCSGG	2 cut(s)	162, 441
BcoDI	GTCTC	1 cut(s)	108
BfaI	CTAG	2 cut(s)	114, 573
BfoI	RGCGCY	1 cut(s)	66
BglI	GCCNNNNNGGC	1 cut(s)	441
BisI	GCNGC	4 cut(s)	37, 66, 111, 874
BlsI	GCNGC	4 cut(s)	38, 67, 112, 875
Bme1390I	CCNGG	5 cut(s)	162, 271, 441, 611, 902
BmeT110I	CYCGRG	1 cut(s)	179
BmgBI	CACGTC	1 cut(s)	790
BmgT120I	GGNCC	3 cut(s)	176, 338, 613
BmiI	GGNNCC	2 cut(s)	64, 178
BmrFI	CCNGG	5 cut(s)	162, 271, 441, 611, 902
BmsI	GCATC	4 cut(s)	180, 750, 789, 1024
BpiI	GAAGAC	1 cut(s)	1052
BpuEI	CTTGAG	2 cut(s)	536, 668
BpuMI	CCSGG	2 cut(s)	162, 441
BsaBI	GATNNNNATC	1 cut(s)	468
BsaHI	GRCGYC	1 cut(s)	63
BsaJI	CCNNGG	6 cut(s)	160, 269, 609, 616, 900, 1081
BsaXI	ACNNNNNCTCC	4 cut(s)	41, 71, 121, 151
Bsc4I	CCNNNNNNNGG	2 cut(s)	114, 167
Bse8I	GATNNNNATC	1 cut(s)	468
BseBI	CCWGG	3 cut(s)	271, 611, 902
BseDI	CCNNGG	6 cut(s)	160, 269, 609, 616, 900, 1081
BseGI	GGATG	1 cut(s)	1039
BseJI	GATNNNNATC	1 cut(s)	468
BseLI	CCNNNNNNNGG	2 cut(s)	114, 167
BseRI	GAGGAG	3 cut(s)	82, 85, 88
BseXI	GCAGC	2 cut(s)	122, 860
BseYI	CCCAGC	2 cut(s)	9, 839
Bsh1236I	CGCG	2 cut(s)	24, 68
BshFI	GGCC	6 cut(s)	177, 340, 347, 587, 614, 675
BshNI	GGYRCC	1 cut(s)	62
BsiHKAI	GWGCWC	1 cut(s)	1008
BsiHKCI	CYCGRG	1 cut(s)	179
BsiSI	CCGG	3 cut(s)	60, 162, 440
BslFI	GGGAC	1 cut(s)	209
BslI	CCNNNNNNNGG	2 cut(s)	114, 167
BsmAI	GTCTC	1 cut(s)	108
BsmBI	CGTCTC	1 cut(s)	108
BsmFI	GGGAC	1 cut(s)	209
BsnI	GGCC	6 cut(s)	177, 340, 347, 587, 614, 675
BsoBI	CYCGRG	1 cut(s)	179
Bsp1286I	GDGCHC	1 cut(s)	1008
Bsp1407I	TGTACA	1 cut(s)	913
Bsp143I	GATC	7 cut(s)	141, 183, 265, 469, 555, 920, 1038
Bsp19I	CCATGG	1 cut(s)	616
BspACI	CCGC	4 cut(s)	37, 66, 108, 147
BspANI	GGCC	6 cut(s)	177, 340, 347, 587, 614, 675
BspFNI	CGCG	2 cut(s)	24, 68
BspLI	GGNNCC	2 cut(s)	64, 178
BspPI	GGATC	3 cut(s)	260, 928, 1033
BspT107I	GGYRCC	1 cut(s)	62
BsrBI	CCGCTC	1 cut(s)	39
BsrGI	TGTACA	1 cut(s)	913
BssECI	CCNNGG	6 cut(s)	160, 269, 609, 616, 900, 1081
BssMI	GATC	7 cut(s)	141, 183, 265, 469, 555, 920, 1038
BssNI	GRCGYC	1 cut(s)	63
BssT1I	CCWWGG	2 cut(s)	616, 1081
Bst2UI	CCWGG	3 cut(s)	271, 611, 902
Bst4CI	ACNGT	2 cut(s)	509, 1070
Bst6I	CTCTTC	2 cut(s)	130, 161
BstACI	GRCGYC	1 cut(s)	63
BstAUI	TGTACA	1 cut(s)	913
BstDEI	CTNAG	1 cut(s)	389
BstDSI	CCRYGG	1 cut(s)	616
BstF5I	GGATG	1 cut(s)	1039
BstFNI	CGCG	2 cut(s)	24, 68
BstH2I	RGCGCY	1 cut(s)	66
BstHHI	GCGC	3 cut(s)	24, 65, 839
BstKTI	GATC	7 cut(s)	144, 186, 268, 472, 558, 923, 1041
BstMAI	GTCTC	1 cut(s)	108
BstMBI	GATC	7 cut(s)	141, 183, 265, 469, 555, 920, 1038
BstMWI	GCNNNNNNNGC	3 cut(s)	19, 441, 593
BstNI	CCWGG	3 cut(s)	271, 611, 902
BstSCI	CCNGG	5 cut(s)	160, 269, 439, 609, 900
BstUI	CGCG	2 cut(s)	24, 68
BstV1I	GCAGC	2 cut(s)	122, 860
BstV2I	GAAGAC	1 cut(s)	1052
BstX2I	RGATCY	1 cut(s)	265
BstXI	CCANNNNNNTGG	1 cut(s)	617
BstYI	RGATCY	1 cut(s)	265
BsuRI	GGCC	6 cut(s)	177, 340, 347, 587, 614, 675
BtgI	CCRYGG	1 cut(s)	616
BtrI	CACGTC	1 cut(s)	790
BtsCI	GGATG	1 cut(s)	1039
BtsIMutI	CAGTG	1 cut(s)	514
CaiI	CAGNNNCTG	1 cut(s)	929
CfoI	GCGC	3 cut(s)	24, 65, 839
Cfr13I	GGNCC	3 cut(s)	176, 338, 613
CseI	GACGC	1 cut(s)	283
Csp6I	GTAC	5 cut(s)	311, 712, 914, 1066, 1071
CspCI	CAANNNNNGTGG	2 cut(s)	337, 372
CviAII	CATG	5 cut(s)	291, 617, 917, 988, 1010
CviQI	GTAC	5 cut(s)	311, 712, 914, 1066, 1071
DdeI	CTNAG	1 cut(s)	389
DinI	GGCGCC	1 cut(s)	64
DpnI	GATC	7 cut(s)	143, 185, 267, 471, 557, 922, 1040
DpnII	GATC	7 cut(s)	141, 183, 265, 469, 555, 920, 1038
EaeI	YGGCCR	2 cut(s)	345, 585
Eam1104I	CTCTTC	2 cut(s)	130, 161
EarI	CTCTTC	2 cut(s)	130, 161
Ecl136II	GAGCTC	1 cut(s)	1006
Eco130I	CCWWGG	2 cut(s)	616, 1081
Eco24I	GRGCYC	1 cut(s)	1008
Eco32I	GATATC	1 cut(s)	934
Eco53kI	GAGCTC	1 cut(s)	1006
Eco57I	CTGAAG	3 cut(s)	240, 927, 1064
Eco88I	CYCGRG	1 cut(s)	179
EcoICRI	GAGCTC	1 cut(s)	1006
EcoRII	CCWGG	3 cut(s)	269, 609, 900
EcoRV	GATATC	1 cut(s)	934
EcoT14I	CCWWGG	2 cut(s)	616, 1081
EcoT38I	GRGCYC	1 cut(s)	1008
EgeI	GGCGCC	1 cut(s)	64
EheI	GGCGCC	1 cut(s)	64
ErhI	CCWWGG	2 cut(s)	616, 1081
Esp3I	CGTCTC	1 cut(s)	108
FaeI	CATG	5 cut(s)	294, 620, 920, 991, 1013
FalI	AAGNNNNNCTT	2 cut(s)	314, 346
FaqI	GGGAC	1 cut(s)	209
FatI	CATG	5 cut(s)	290, 616, 916, 987, 1009
FauI	CCCGC	1 cut(s)	115
Fnu4HI	GCNGC	4 cut(s)	37, 66, 111, 874
FokI	GGATG	1 cut(s)	1046
FriOI	GRGCYC	1 cut(s)	1008
Fsp4HI	GCNGC	4 cut(s)	37, 66, 111, 874
FspBI	CTAG	2 cut(s)	114, 573
GlaI	GCGC	3 cut(s)	23, 64, 838
GluI	GCNGC	4 cut(s)	37, 66, 111, 874
GsaI	CCCAGC	2 cut(s)	13, 843
HaeII	RGCGCY	1 cut(s)	66
HaeIII	GGCC	6 cut(s)	177, 340, 347, 587, 614, 675
HapII	CCGG	3 cut(s)	60, 162, 440
HgaI	GACGC	1 cut(s)	283
HhaI	GCGC	3 cut(s)	24, 65, 839
Hin1I	GRCGYC	1 cut(s)	63
Hin1II	CATG	5 cut(s)	294, 620, 920, 991, 1013
Hin6I	GCGC	3 cut(s)	22, 63, 837
HinP1I	GCGC	3 cut(s)	22, 63, 837
HinfI	GANTC	6 cut(s)	70, 191, 229, 305, 451, 1027
HpaII	CCGG	3 cut(s)	60, 162, 440
HphI	GGTGA	5 cut(s)	136, 541, 827, 896, 976
Hpy166II	GTNNAC	1 cut(s)	512
Hpy188I	TCNGA	6 cut(s)	32, 141, 212, 288, 422, 960
Hpy188III	TCNNGA	4 cut(s)	226, 553, 1031, 1042
Hpy8I	GTNNAC	1 cut(s)	512
Hpy99I	CGWCG	2 cut(s)	170, 791
HpyAV	CCTTC	1 cut(s)	446
HpyCH4III	ACNGT	2 cut(s)	509, 1070
HpyCH4IV	ACGT	1 cut(s)	789
HpyCH4V	TGCA	6 cut(s)	413, 638, 661, 704, 876, 1055
HpyF10VI	GCNNNNNNNGC	3 cut(s)	19, 441, 593
HpyF3I	CTNAG	1 cut(s)	389
HpySE526I	ACGT	1 cut(s)	789
Hsp92I	GRCGYC	1 cut(s)	63
Hsp92II	CATG	5 cut(s)	294, 620, 920, 991, 1013
HspAI	GCGC	3 cut(s)	22, 63, 837
KasI	GGCGCC	1 cut(s)	62
Kzo9I	GATC	7 cut(s)	141, 183, 265, 469, 555, 920, 1038
LmnI	GCTCC	4 cut(s)	44, 256, 1003, 1011
Lsp1109I	GCAGC	2 cut(s)	122, 860
LweI	GCATC	4 cut(s)	180, 750, 789, 1024
MaeI	CTAG	2 cut(s)	114, 573
MaeII	ACGT	1 cut(s)	789
MaeIII	GTNAC	4 cut(s)	428, 475, 827, 982
MalI	GATC	7 cut(s)	143, 185, 267, 471, 557, 922, 1040
MbiI	CCGCTC	1 cut(s)	39
MboI	GATC	7 cut(s)	141, 183, 265, 469, 555, 920, 1038
MboII	GAAGA	4 cut(s)	117, 148, 251, 1057
MfeI	CAATTG	1 cut(s)	1050
MflI	RGATCY	1 cut(s)	265
MhlI	GDGCHC	1 cut(s)	1008
MlsI	TGGCCA	1 cut(s)	347
MluCI	AATT	5 cut(s)	639, 748, 946, 1050, 1119
MluNI	TGGCCA	1 cut(s)	347
Mly113I	GGCGCC	1 cut(s)	63
MlyI	GAGTC	2 cut(s)	200, 445
MmeI	TCCRAC	1 cut(s)	983
Mox20I	TGGCCA	1 cut(s)	347
MroXI	GAANNNNTTC	1 cut(s)	74
MscI	TGGCCA	1 cut(s)	347
MseI	TTAA	2 cut(s)	402, 863
Msp20I	TGGCCA	1 cut(s)	347
MspA1I	CMGCKG	1 cut(s)	1076
MspI	CCGG	3 cut(s)	60, 162, 440
MspR9I	CCNGG	5 cut(s)	162, 271, 441, 611, 902
MunI	CAATTG	1 cut(s)	1050
MvaI	CCWGG	3 cut(s)	271, 611, 902
MvnI	CGCG	2 cut(s)	24, 68
MwoI	GCNNNNNNNGC	3 cut(s)	19, 441, 593
NarI	GGCGCC	1 cut(s)	63
NciI	CCSGG	2 cut(s)	162, 441
NcoI	CCATGG	1 cut(s)	616
NdeII	GATC	7 cut(s)	141, 183, 265, 469, 555, 920, 1038
NlaIII	CATG	5 cut(s)	294, 620, 920, 991, 1013
NlaIV	GGNNCC	2 cut(s)	64, 178
NmeAIII	GCCGAG	2 cut(s)	214, 613
NmuCI	GTSAC	3 cut(s)	475, 827, 982
PcsI	WCGNNNNNNNCGW	1 cut(s)	192
PdmI	GAANNNNTTC	1 cut(s)	74
PfeI	GAWTC	4 cut(s)	70, 229, 305, 1027
PkrI	GCNGC	4 cut(s)	38, 67, 112, 875
PleI	GAGTC	2 cut(s)	199, 445
PluTI	GGCGCC	1 cut(s)	66
PpsI	GAGTC	2 cut(s)	199, 445
Psp124BI	GAGCTC	1 cut(s)	1008
Psp6I	CCWGG	3 cut(s)	269, 609, 900
PspFI	CCCAGC	2 cut(s)	9, 839
PspGI	CCWGG	3 cut(s)	269, 609, 900
PspN4I	GGNNCC	2 cut(s)	64, 178
PspPI	GGNCC	3 cut(s)	176, 338, 613
PstNI	CAGNNNCTG	1 cut(s)	929
PsuI	RGATCY	1 cut(s)	265
PvuII	CAGCTG	1 cut(s)	1076
RsaI	GTAC	5 cut(s)	312, 713, 915, 1067, 1072
RsaNI	GTAC	5 cut(s)	311, 712, 914, 1066, 1071
SacI	GAGCTC	1 cut(s)	1008
SaqAI	TTAA	2 cut(s)	402, 863
SatI	GCNGC	4 cut(s)	37, 66, 111, 874
Sau3AI	GATC	7 cut(s)	141, 183, 265, 469, 555, 920, 1038
Sau96I	GGNCC	3 cut(s)	176, 338, 613
SchI	GAGTC	2 cut(s)	200, 445
ScrFI	CCNGG	5 cut(s)	162, 271, 441, 611, 902
SduI	GDGCHC	1 cut(s)	1008
SfaNI	GCATC	4 cut(s)	180, 750, 789, 1024
SfoI	GGCGCC	1 cut(s)	64
SmlI	CTYRAG	2 cut(s)	551, 683
SmoI	CTYRAG	2 cut(s)	551, 683
Sse9I	AATT	5 cut(s)	639, 748, 946, 1050, 1119
SsiI	CCGC	4 cut(s)	37, 66, 108, 147
SspDI	GGCGCC	1 cut(s)	62
SspI	AATATT	1 cut(s)	880
SspMI	CTAG	2 cut(s)	114, 573
SstI	GAGCTC	1 cut(s)	1008
StyD4I	CCNGG	5 cut(s)	160, 269, 439, 609, 900
StyI	CCWWGG	2 cut(s)	616, 1081
TaaI	ACNGT	2 cut(s)	509, 1070
TaiI	ACGT	1 cut(s)	792
TaqI	TCGA	1 cut(s)	786
TaqII	GACCGA	1 cut(s)	1115
TasI	AATT	5 cut(s)	639, 748, 946, 1050, 1119
TatI	WGTACW	1 cut(s)	913
TauI	GCSGC	2 cut(s)	39, 68
TfiI	GAWTC	4 cut(s)	70, 229, 305, 1027
Tru1I	TTAA	2 cut(s)	402, 863
Tru9I	TTAA	2 cut(s)	402, 863
TscAI	CASTG	1 cut(s)	514
TseFI	GTSAC	3 cut(s)	475, 827, 982
TseI	GCWGC	2 cut(s)	110, 873
Tsp45I	GTSAC	3 cut(s)	475, 827, 982
TspDTI	ATGAA	1 cut(s)	590
TspGWI	ACGGA	4 cut(s)	184, 328, 473, 509
TspRI	CASTG	1 cut(s)	514
XapI	RAATTY	1 cut(s)	946
XmnI	GAANNNNTTC	1 cut(s)	74
XspI	CTAG	2 cut(s)	114, 573

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.